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Jingjing Huang, Adnan Khan Niazi, David Young, Leonardo Astolfi Rosado, Didier Vertommen, Nandita Bodra, Mohamed Ragab Abdelgawwad, Florence Vignols, Bo Wei, Khadija Wahni, Talaat Bashandy, Laetitia Bariat, Frank Van Breusegem, Joris Messens, Jean-Philippe Reichheld
Plant malate dehydrogenase (MDH) isoforms are found in different cell compartments and function in key metabolic pathways. It is well known that the chloroplastic NADP-dependent MDH activities are strictly redox regulated and controlled by light. However, redox dependence of other NAD-dependent MDH isoforms have been less studied. Here, we show by in vitro biochemical characterization that the major cytosolic MDH isoform (cytMDH1) is sensitive to H2O2 through sulfur oxidation of cysteines and methionines. CytMDH1 oxidation affects the kinetics, secondary structure, and thermodynamic stability of cytMDH1...
November 29, 2017: Journal of Experimental Botany
F Eggenberger, M Rowell-Rahier
No abstract text is available yet for this article.
July 1991: Die Naturwissenschaften
Danilo M Daloso, Karolin Müller, Toshihiro Obata, Alexandra Florian, Takayuki Tohge, Alexandra Bottcher, Christophe Riondet, Laetitia Bariat, Fernando Carrari, Adriano Nunes-Nesi, Bob B Buchanan, Jean-Philippe Reichheld, Wagner L Araújo, Alisdair R Fernie
Plant mitochondria have a fully operational tricarboxylic acid (TCA) cycle that plays a central role in generating ATP and providing carbon skeletons for a range of biosynthetic processes in both heterotrophic and photosynthetic tissues. The cycle enzyme-encoding genes have been well characterized in terms of transcriptional and effector-mediated regulation and have also been subjected to reverse genetic analysis. However, despite this wealth of attention, a central question remains unanswered: "What regulates flux through this pathway in vivo?" Previous proteomic experiments with Arabidopsis discussed below have revealed that a number of mitochondrial enzymes, including members of the TCA cycle and affiliated pathways, harbor thioredoxin (TRX)-binding sites and are potentially redox-regulated...
March 17, 2015: Proceedings of the National Academy of Sciences of the United States of America
Luca Paolino, Laurent Genser, Sylvie Fritsch, Nicola De' Angelis, Daniel Azoulay, Andrea Lazzati
BACKGROUND: Health-related information on the Internet is constantly increasing, but its quality and accountability are difficult to assess. Patients browse the Net to get more information, but the impact of the Internet on their decisions about surgical techniques, referral centers, or surgeon choice are still not clear. This study aimed to describe the role of the Internet in the decision-making process of obese patients seeking bariatric surgery. METHODS: Two hundred and twelve candidates for bariatric surgery were asked to answer a questionnaire evaluating their access to the Internet, the usefulness and trustworthiness of Internet-retrieved information, the verification of the information, and the role of the information in the decision-making process...
April 2015: Obesity Surgery
V M Sedov, M B Fishman
Nowadays, according to data of WHO, the diabetes mellitus was diagnosed in more than 280 million people. The diabetes mellitus type II had 90% patients. The applied methods of conservative therapy seldom lead to euglycemia condition of patients. Last years the treatment of diabetes mellitus was carried out by the method of different bariatic interventions. Good results was obtained, they should be analyzed and investigate. The results of treatment of 142 patients from 628 patients (with type II) were estimated...
2013: Vestnik Khirurgii Imeni I. I. Grekova
Corinne Marchal, Valérie Delorme-Hinoux, Laetitia Bariat, Wafi Siala, Christophe Belin, Julio Saez-Vasquez, Christophe Riondet, Jean-Philippe Reichheld
Thioredoxins (TRX) are key components of cellular redox balance, regulating many target proteins through thiol/disulfide exchange reactions. In higher plants, TRX constitute a complex multigenic family whose members have been found in almost all cellular compartments. Although chloroplastic and cytosolic TRX systems have been largely studied, the presence of a nuclear TRX system has been elusive for a long time. Nucleoredoxins (NRX) are potential nuclear TRX found in most eukaryotic organisms. In contrast to mammals, which harbor a unique NRX, angiosperms generally possess multiple NRX organized in three subfamilies...
January 2014: Molecular Plant
J A Zirrolli, E Davoli, L Bettazzoli, M Gross, R C Murphy
The mass spectra of products found by collisional activation of selected prostaglandins and thromboxanes were studied by tandem mass spectrometry as barium carboxylate salts and as carboxylate anions. Collision-induced dissociation (CID) of these closed shell ions generated by fast atom bombardment mass spectrometry reveals a wealth of structural information for these hydroxy acids. Decomposition reactions were found to be dependent upon the eicosanoid ring structure and the type of ion being studied, either positive or negative ion...
July 1990: Journal of the American Society for Mass Spectrometry
J S Crockett, M L Gross, W W Christie, R T Holman
High-energy collisional activation (CA) of long-chain fatty acid ions induces decompositions that occur remote from the charge site. These charge-remote fragmentations (CRFs) have been shown to provide much structural information. In this report, the CRF of a continuous series of 12 homoconjugated octadecadienoic acids was studied with fast atom bombardment and tandem mass spectrometry. Each fatty acid was desorbed as the carboxylate anion, [M - H](-), the dilithiated species, [M - H+2Li](+), or the bariated species, [M - H+Ba](+), giving three ways of localizing the charge...
April 1990: Journal of the American Society for Mass Spectrometry
Michael Wilson, Ali Alhamdani, Kamal Mahawar, Maureen Boyle, Peter Small
No abstract text is available yet for this article.
2011: International Journal of Surgery
Ana Ramos-Levi, Andres Sanchez-Pernaute, Pilar Matia, Lucio Cabrerizo, Ana Barabash, Carmen Hernandez, Alfonso Calle-Pascual, Antonio Torres, Miguel Rubio
BACKGROUND: Controversy exists regarding type 2 diabetes (T2D) remission rates after bariatric surgery (BS) due to heterogeneity in its definition and patients' baseline features. We evaluate T2D remission using recent criteria, according to preoperative characteristics and insulin therapy (IT). METHODS: We performed a retrospective study from a cohort of 657 BS from a single center (2006-2011), of which 141 (57.4 % women) had T2D. We evaluated anthropometric and glucose metabolism parameters before surgery and at 1-year follow-up...
October 2013: Obesity Surgery
Cornelis Adrianus Sebastianus Berende, Jean-Paul de Zoete, Johannes Franciscus Smulders, Simon Willem Nienhuijs
Bariatric revision surgery is associated with several complications that can be attributed to decreased quality of tissue and complexity of the surgery. A laparoscopic sleeve gastrectomy is a simple technique with potential advantages. Therefore, the results of this procedure were evaluated as a revisional option. Fifty-one patients underwent laparoscopic sleeve gastrectomy (LSG). Indications for the LSG were insufficient weight loss (34 patients, group 1) or vomiting (17 patients, group 2) following a laparoscopic adjustable gastric banding (LAGB) or vertical banded gastroplasty (VBG)...
February 2012: Obesity Surgery
R Paruch, L Rzeznik, B Czerwinski, B J Garrison, N Winograd, Z Postawa
Coarse-grained molecular dynamics computer simulations are applied to investigate fundamental processes induced by an impact of keV C(60) projectile at an organic overlayer composed of long, well-organized linear molecules. The energy transfer pathways, sputtering yields, and the damage induced in the irradiated system, represented by a Langmuir-Blodgett (LB) multilayers composed from molecules of bariated arachidic acid, are investigated as a function of the kinetic energy and impact angle of the projectile and the thickness of the organic system...
April 9, 2009: Journal of Physical Chemistry. C, Nanomaterials and Interfaces
Athar Yasin, Ameet G Patel
Iatrogenic nerve injuries have been rarely reported following laparoscopic abdominal surgeries. These can be serious complications with the potential for long-term disability. We present a rare case of bilateral sciatic nerve palsy following laparoscopic sleeve gastrectomy on a super-super-obese male with BMI 78.04 kg/m2. The procedure was completed laparoscopically in 7 hours duration, and in spite of adequate precautions, he developed bilateral sciatic nerve palsy. Nerve conduction studies showed denervation of L5/S1 confirming the diagnosis...
July 2007: Obesity Surgery
Philipp Schuetz, Ralph Peterli, Christian Ludwig, Thomas Peters
BACKGROUND: Malabsorptive bariatic surgery for morbid obesity has been very effective in producing weight loss. However, patients may experience some degree of malnutrition, which may lead to various clinical symptoms, such as fatigue and weakness. Morbid obesity is often associated with impaired reproductive function, and weight loss generally improves sexual function in both sexes. However, women with extreme weight loss may experience secondary amenorrhea. In men, zinc deficiency may lead to impaired testosterone synthesis resulting in hypogonadism and impotency...
August 2004: Obesity Surgery
D Zhai, B Din, R Liu, X Hua, H Chen
The experimental results displayed that the cellular immune functions of tumor-bearing mice in control group were significantly lower than that the normal control group. The cellular immune functions of those groups accepting moxibustion treatment were maintained at a relatively higher level. Among them the Guanyuan group seemed to be best in immune regulation. The data further demonstrated that an instant elevation of serum ACTH and beta-EP could be caused by moxibustion. But the bariations of serum ACTH and beta-EP are not paralleled...
1996: Zhen Ci Yan Jiu, Acupuncture Research
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