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https://www.readbyqxmd.com/read/28629938/poly-glycidyl-methacrylate-nanoparticle-coated-capillary-with-oriented-antibody-immobilization-for-immunoaffinity-in-tube-solid-phase-microextraction-preparation-and-characterization
#1
Ling-Ling Ying, Yuan-Chun Ma, Bei Xu, Xian-Hua Wang, Lin-Yi Dong, Dong-Mei Wang, Kun Liu, Liang Xu
A combination between modification with nanoparticles (NP) and oriented antibody immobilization (OAI) on the inner face of capillary was for the first time developed for immunoaffinity in-tube solid-phase microextraction (SPME) to promise high antigen extraction capacity. β2-microglobin (β2MG) and cystatin C (Cys-C) were selected as model antigens. Poly(glycidyl methacrylate) (PGMA) NPs were chemically immobilized onto the capillary by a ring-opening reaction. Antibodies for β2MG and Cys-C were immobilized on the NPs through OAI...
June 12, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28629123/sensory-characteristics-and-volatile-components-of-dry-dog-foods-manufactured-with-sorghum-fractions
#2
Brizio Di Donfrancesco, Kadri Koppel
Descriptive sensory analysis and gas chromatography-mass spectrometry (GC-MS) with a modified headspace solid-phase microextraction (SPME) method was performed on three extruded dry dog food diets manufactured with different fractions of red sorghum and a control diet containing corn, brewer's rice, and wheat as a grain source in order to determine the effect of sorghum fractions on dry dog food sensory properties. The aroma compounds and flavor profiles of samples were similar with small differences, such as higher toasted aroma notes, and musty and dusty flavor in the mill-feed sample...
June 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28623787/simultaneous-determination-of-btex-and-their-metabolites-using-solid-phase-microextraction-followed-by-hplc-or-gc-ms-an-application-in-teeth-as-environmental-biomarkers
#3
Johannes L González, Albert Pell, Montserrat López-Mesas, Manuel Valiente
Applications of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes (BTEX) release them into the environment exposing living organism. These endocrine disruptors are toxic, highly volatile and easily absorbed by the lungs and can cause adverse consequences for the human health as neurological diseases and cancer. A method for the analysis of BTEX and its metabolites (phenols and aromatic acids) in teeth is presented. The method consists in a one-step simple extraction procedure from spiked tooth using NaOH solution followed by SPME-HPLC or HS-SPME-GC/MS determination...
June 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28618092/a-platinized-stainless-steel-fiber-with-in-situ-coated-polyaniline-polypyrrole-graphene-oxide-nanocomposite-sorbent-for-headspace-solid-phase-microextraction-of-aliphatic-aldehydes-in-rice-samples
#4
Alireza Ghiasvand, Afagh Nasirian, Samira Koonani, Kolsoum Nouriasl
The surface of a stainless steel fiber was made larger, porous and cohesive by platinizing for tightly attachment of its coating. Then, it was coated by a polyaniline/polypyrrole/graphene oxide (PANI/PP/GO) nanocomposite film using electrochemical polymerization (EP) process. The prepared PANI/PP/GO fiber was used for headspace solid-phase microextraction (HS-SPME) of linear aliphatic aldehydes in rice samples followed by gas-chromatography flame ionization detector (GC-FID) determination. To achieve the highest extraction efficiency, various experimental parameters including extraction time and temperature, matrix modifier, and desorption condition were studied...
June 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28617451/biological-and-chemical-strategies-for-exploring-inter-and-intra-kingdom-communication-mediated-via-bacterial-volatile-signals
#5
Mohamed A Farag, Geun Cheol Song, Yong-Soon Park, Bianca Audrain, Soohyun Lee, Jean-Marc Ghigo, Joseph W Kloepper, Choong-Min Ryu
Airborne chemical signals emitted by bacteria influence the behavior of other bacteria and plants. We present an overview of in vitro methods for evaluating bacterial and plant responses to bacterial volatile compounds (BVCs). Three types of equipment have been used to physically separate the bacterial test strains from either other bacterial strains or plants (in our laboratory we use either Arabidopsis or tobacco plant seedlings): a Petri dish containing two compartments (BI Petri dish); two Petri dishes connected with tubing; and a microtiter-based assay...
July 2017: Nature Protocols
https://www.readbyqxmd.com/read/28616692/a-minimally-invasive-method-for-profiling-volatile-organic-compounds-within-postmortem-internal-gas-reservoirs
#6
Katelynn A Perrault, Pierre-Hugues Stefanuto, Lena M Dubois, Vincent Varlet, Silke Grabherr, Jean-François Focant
In forensic casework, non-invasive and minimally-invasive methods for postmortem examinations are extremely valuable. Whole body postmortem computed tomography (PMCT) is often used to provide visualization of the internal characteristics of a body prior to more invasive procedures and has also been used to locate gas reservoirs inside the body to assist in determining cause of death. Preliminary studies have demonstrated that exploiting the volatile organic compounds (VOCs) located in these gas reservoirs by comprehensive two-dimensional gas chromatography-high-resolution time-of-flight mass spectrometry (GC×GC-HRTOF-MS) may assist in providing information regarding the postmortem interval...
June 15, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28602302/fabrication-of-porous-ionic-liquid-polymer-as-solid-phase-microextraction-coating-for-analysis-of-organic-acids-by-gas-chromatography-mass-spectrometry
#7
Zhentao Tang, Yixiang Duan
A kind of porous ionic liquid polymer was designed as coating material for solid-phase microextraction (SPME) fibers to extract polar organic acids. The synthesized ionic liquids (1-vinyl-3-(4-vinyl-benzyl)imidazolium chloride) were polymerized in the presence of azobisisobutyronitrile (AIBN), and subsequently coated on stainless steel wires with physical adhesion. To guarantee the adhesion between coating and the surface of stainless steel wires, micro-wave induced plasma was applied to modify the stainless steel wires...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28602289/fabrication-of-polyaniline-silver-composite-coating-as-a-dual-functional-platform-for-microextraction-and-matrix-free-laser-desorption-ionization
#8
Siming Huang, Jianqiao Xu, Xuan Tao, Xiao Chen, Fang Zhu, Ying Wang, Ruifen Jiang, Gangfeng Ouyang
Polyaniline/silver (PANI/Ag) composites were prepared with a one-step synthesis method, and fabricated into solid-phase microextraction fiber coatings through three different means, i.e. in situ growth, gluing and chemical bonding. This work explored the utility of the as-fabricated PANI/Ag SPME fibers as dual-functional platforms for microextraction and matrix-free laser desorption/ionization (LDI) of the target analyte. The results showed that after incorporating Ag into the PANI polymer, the LDI efficiency was improved by over two times, which was 92...
September 1, 2017: Talanta
https://www.readbyqxmd.com/read/28597921/feasibility-of-canine-detection-of-mass-storage-devices-a-study-of-volatile-organic-compounds-emanating-from-electronic-devices-using-solid-phase-microextraction
#9
Lauryn E DeGreeff, Michelle Cerreta, Mark Rispoli
Detection of canines are well-known to be valuable in the location of contraband, such as explosives or narcotics. More recently, canines have been trained and utilized in the detection of concealed mass storage devices that might contain evidence of illegal activity such as child pornography. To lay the analytical foundation for this detection work, research was carried out to determine the volatile organic compounds associated with mass storage devices (MSD) that could be used by trained canines for detection...
June 9, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28597472/aroma-volatile-profile-of-black-morel-morchella-importuna-grown-in-israel
#10
Zipora Tietel, Segula Masaphy
BACKGROUND: Headspace solid-phase microextraction (HS-SPME) method with gas-chromatography mass spectrometry (GC-MS) was used to profile the aroma volatiles of mature fruiting bodies of Morchella importuna grown in Israel. RESULTS: We tentatively identified 40 aroma compounds and seven unknown volatiles. The M. importuna aroma profile consisted of 14 aldehydes, 6 alcohols, 10 methyl esters, 4 heterocyclic/sulfur compounds, 10 carbohydrates and 3 other compounds-one acid, one ketone and one butyl ester...
June 9, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28590915/cancer-sniffer-dogs-how-can-we-translate-this-peculiarity-in-laboratory-medicine-results-of-a-pilot-study-on-gastrointestinal-cancers
#11
Concetta Panebianco, Edgar Kelman, Kristel Vene, Domenica Gioffreda, Francesca Tavano, Raivo Vilu, Fulvia Terracciano, Illar Pata, Kaarel Adamberg, Angelo Andriulli, Valerio Pazienza
BACKGROUND: Identification of cancer biomarkers to allow early diagnosis is an urgent need for many types of tumors, whose prognosis strongly depends on the stage of the disease. Canine olfactory testing for detecting cancer is an emerging field of investigation. As an alternative, here we propose to use GC-Olfactometry (GC/O), which enables the speeding up of targeted biomarker identification and analysis. A pilot study was conducted in order to determine odor-active compounds in urine that discriminate patients with gastrointestinal cancers from control samples (healthy people)...
June 7, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28587778/advanced-method-optimization-for-volatile-aroma-profiling-of-beer-using-two-dimensional-gas-chromatography-time-of-flight-mass-spectrometry
#12
Pierre-Hugues Stefanuto, Katelynn A Perrault, Lena M Dubois, Benjamin L'Homme, Catherine Allen, Caitriona Loughnane, Nobuo Ochiai, Jean-François Focant
The complex mixture of volatile organic compounds (VOCs) present in the headspace of Trappist and craft beers was studied to illustrate the efficiency of thermal desorption (TD) comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS) for highlighting subtle differences between highly complex mixtures of VOCs. Headspace solid-phase microextraction (HS-SPME), multiple (and classical) stir bar sorptive extraction (mSBSE), static headspace (SHS), and dynamic headspace (DHS) were compared for the extraction of a set of 21 representative flavor compounds of beer aroma...
June 1, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28587077/floral-scent-chemistry-of-luculia-yunnanensis-rubiaceae-a-species-endemic-to-china-with-sweetly-fragrant-flowers
#13
Yuying Li, Youming Wan, Zhenghai Sun, Taiqiang Li, Xiongfang Liu, Hong Ma, Xiuxian Liu, Rui He, Yan Ma, Zhenghong Li
Luculia plants are famed ornamentals with sweetly fragrant flowers. Luculia yunnanensis Hu is an endemic plant from Yunnan Province, China. Headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to identify the volatile organic compounds (VOCs) of the different flower development stages of L. yunnanensis for the evaluation of floral volatile polymorphism. The results showed that a total of 40 compounds were identified at four different stages. The main aroma-active compounds were 3-carene, α-cubebene, α-copaene, δ-cadinene, and isoledene...
May 25, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28578069/model-studies-on-the-formation-of-volatile-compounds-generated-by-a-thermal-treatment-of-steryl-esters-with-different-fatty-acid-moieties
#14
Marianna Raczyk, Dominik Kmiecik, Peter Schieberle, Roman Przybylski, Henryk Jeleń, Magdalena Rudzińska
The consumption of plant sterols is reported to have a beneficial effects on human health, i.e. phytosterols are known for their cholesterol-lowering properties. Whereas, they are prone to oxidation and currently there is ongoing worldwide research aimed at the biological effect of phytosterol oxides. In this study volatile compounds formed during thermal degradation of stigmasteryl esters were identified. The research was conducted using standards of stigmasterol, fatty acids and stigmasteryl esters as well as fat enriched with stigmasteryl esters which were thermally treated at 60°C and 180°C for 12h...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28578066/microbial-metabolites-profile-during-in-vitro-human-colonic-fermentation-of-breakfast-menus-consumed-by-mexican-school-children
#15
Victor Manuel Zamora-Gasga, Efigenia Montalvo-González, Guadalupe Loarca-Piña, Pedro Alberto Vázquez-Landaverde, Juscelino Tovar, Sonia G Sáyago-Ayerdi
The nutrition transition promotes the development of childhood obesity. Currently, Mexico is affected by this serious public health problem. The nutritional and functional characterization of a whole menu has a number of advantages over the study of single nutrients. Since breakfast is considered the most important meal of the day, this study aimed to evaluate the metabolite profile produced by in vitro human colonic fermentation of the isolated indigestible fraction (IF) from three different Mexican breakfast (M-B) menus (Modified "MM-B", traditional "TM-B", and alternative "AM-B"), previously identified as commonly consumed by Mexican schoolchildren in Nayarit State, Mexico...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28578064/effect-of-liquid-smoking-on-lipid-hydrolysis-and-oxidation-reactions-during-in-vitro-gastrointestinal-digestion-of-european-sea-bass
#16
Bárbara Nieva-Echevarría, Encarnación Goicoechea, María D Guillén
The effect of smoking using liquid smoke flavourings on the hydrolysis and oxidation of European sea bass lipids during in vitro digestion was investigated. The techniques used were (1)H NMR and SPME-GC/MS. The former proved that liquid smoking does not influence the extent of lipolysis, but prevents those lipid oxidation reactions that occur during in vitro digestion of unsmoked samples, giving rise to cis,trans-conjugated dienes associated with hydroperoxy/hydroxy groups. SPME-GC/MS corroborated the results obtained by (1)H NMR in relation to the antioxidant effect of smoking under gastrointestinal conditions...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28578030/behaviour-of-non-oxidized-and-oxidized-flaxseed-oils-as-models-of-omega-3-rich-lipids-during-in-vitro-digestion-occurrence-of-epoxidation-reactions
#17
Bárbara Nieva-Echevarría, Encarnación Goicoechea, María D Guillén
Fresh and partially oxidized flaxseed oil, as models of omega-3 rich lipids, were submitted to in vitro gastrointestinal digestion. Hydrolysis level, lipid composition and oxidative status of the samples before and after digestion were studied by Proton Nuclear Magnetic Resonance ((1)H NMR) and Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry (SPME-GC/MS). Although a great degree of lipolysis was reached in both kinds of samples after digestion, it was somewhat lower in the digests of oxidized flaxseed oil...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28571710/identification-of-odorant-binding-proteins-in-carpomya-vesuviana-and-their-binding-affinity-to-the-male-borne-semiochemicals-and-host-plant-volatiles
#18
Yawei Li, Ping Zhou, Junhua Zhang, Ding Yang, Zhihong Li, Xianglin Zhang, Shuifang Zhu, Yanxue Yu, Naizhong Chen
Carpomya vesuviana (Costa; Diptera: Tephritidae) is an agricultural pest that causes serious damage to jujube fruits. However, the mechanism of olfaction, which is critical for host identification, is not well understood in this pest. In this study, we have identified for the first time five protein types involved in the olfactory signal transduction of C. vesuviana by using transcriptome sequencing. These include 6 odorant-binding proteins (OBPs), 15 odorant receptors (ORs), 22 gustatory receptors (GRs), 2 chemosensory proteins (CSPs), and 2 sensory neuron membrane proteins (SNMPs)...
May 29, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28560053/volatiles-profiling-in-ceratonia-siliqua-carob-bean-from-egypt-and-in-response-to-roasting-as-analyzed-via-solid-phase-microextraction-coupled-to-chemometrics
#19
Mohamed A Farag, Dina M El-Kersh
Ceratonia siliqua is a legume tree of considerable commercial importance for the flavor and sweets industry cultivated mostly for its pods nutritive value and or several health benefits. Despite extensive studies on C. siliqua pod non-volatile metabolites, much less is known regarding volatiles composition which contributes to the flavor of its many food products. To gain insight into C. siliqua aroma, 31 volatile constituents from unroasted and roasted pods were profiled using headspace solid-phase micro extraction (HD-SPME) analyzed via quadruple mass spectrometer followed by multivariate data analyses...
July 2017: Journal of Advanced Research
https://www.readbyqxmd.com/read/28559738/differentiation-of-commercial-pdo-wines-produced-in-istria-croatia-according-to-variety-and-harvest-year-based-on-hs-spme-gc-ms-volatile-aroma-compound-profiling
#20
Igor Lukić, Ivana Horvat
To differentiate monovarietal wines made from native and introduced varieties in Istria (Croatia), samples of Malvazija istarska, Chardonnay and Muscat yellow from two harvest years (2013 and 2014) were subjected to headspace solid-phase microextraction and gas chromatographic/mass spectrometric analysis (HS-SPME-GC/MS) of volatile aroma compounds. Significant effects of variety and harvest year were determined, but their interaction complicated the differentiation. Particular compounds were consistent as markers of variety in both years: nerol for Malvazija, ethyl cinnamate and a tentatively identified isomer of dimethylbenzaldehyde for Chardonnay, and terpenes for Muscat yellow...
March 2017: Food Technology and Biotechnology
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