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https://www.readbyqxmd.com/read/28449085/guanine-quadruplex-monoclonal-antibody-1h6-cross-reacts-with-restrained-thymidine-rich-single-stranded-dna
#1
Hinke G Kazemier, Katrin Paeschke, Peter M Lansdorp
Previously we reported the production and characterization of monoclonal antibody 1H6 raised against (T4G4)2 intermolecular guanine quadruplex (G4) DNA structures (Henderson A. et al. (2014) Nucleic Acids Res., 42, 860-869; Hoffmann R.F. et al. (2016) Nucleic Acids Res., 44, 152-163). It was shown that 1H6 strongly stains nuclei and has exquisite specificity for heterochromatin by immuno-electron microscopy. Here we extend our studies of 1H6 reactivity using enzyme-linked immunosorbent assay (ELISA) and microscale thermophoresis (MST)...
April 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28448516/natural-variation-of-pirna-expression-affects-immunity-to-transposable-elements
#2
Sergei Ryazansky, Elizaveta Radion, Anastasia Mironova, Natalia Akulenko, Yuri Abramov, Valeriya Morgunova, Maria Y Kordyukova, Ivan Olovnikov, Alla Kalmykova
In the Drosophila germline, transposable elements (TEs) are silenced by PIWI-interacting RNA (piRNA) that originate from distinct genomic regions termed piRNA clusters and are processed by PIWI-subfamily Argonaute proteins. Here, we explore the variation in the ability to restrain an alien TE in different Drosophila strains. The I-element is a retrotransposon involved in the phenomenon of I-R hybrid dysgenesis in Drosophila melanogaster. Genomes of R strains do not contain active I-elements, but harbour remnants of ancestral I-related elements...
April 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28448491/relevant-units-of-analysis-for-applied-and-basic-research-dealing-with-neglected-transmissible-diseases-the-predominant-clonal-evolution-model-of-pathogenic-microorganisms
#3
REVIEW
Michel Tibayrenc, Francisco J Ayala
The predominant clonal evolution (PCE) model seeks to formulate a common population genetics framework for all micropathogens (namely, parasitic protozoa, fungi and yeasts, bacteria, and viruses). It relies on a definition of clonality that is only based on population structure features (namely, strongly restrained genetic recombination). Its clear-cut properties make it of strong interest for applied and basic research, since it permits the definition of stable, clearly delimited units of analysis below the species level: clonal genotypes and discrete genetic subdivisions ("near-clades")...
April 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28447727/advanced-glycation-end-products%C3%A2-induced-mitochondrial-energy-metabolism-dysfunction-alters-proliferation-of-human-umbilical-vein-endothelial-cells
#4
Yuan Li, Ye Chang, Ning Ye, Yintao Chen, Naijin Zhang, Yingxian Sun
Advanced glycation end products (AGEs) restrain the proliferation of endothelial cells, which is an important determinant of diabetic vasculopathy. Mitochondrial biogenesis serves an essential role in cellular adaptation and repair. The current study aimed to investigate alterations in mitochondrial energy metabolism in human umbilical vein endothelial cells (HUVECs) and the latent mechanism regulated by AGEs. The proliferation of cultured HUVECs stimulated with AGEs was detected using an MTT assay and a real‑time cell analyzer (RTCA)...
March 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28447099/endogenous-developmental-endothelial-locus-1-limits-ischaemia-related-angiogenesis-by-blocking-inflammation
#5
Anne Klotzsche-von Ameln, Sebastian Cremer, Jedrzej Hoffmann, Peggy Schuster, Sherif Khedr, Irina Korovina, Maria Troullinaki, Ales Neuwirth, David Sprott, Antonios Chatzigeorgiou, Matina Economopoulou, Alessia Orlandi, Andreas Hain, Andreas M Zeiher, Andreas Deussen, George Hajishengallis, Stefanie Dimmeler, Triantafyllos Chavakis, Emmanouil Chavakis
We have recently identified endothelial cell-secreted developmental endothelial locus-1 (Del-1) as an endogenous inhibitor of β2-integrin-dependent leukocyte infiltration. Del-1 was previously also implicated in angiogenesis. Here, we addressed the role of endogenously produced Del-1 in ischaemia-related angiogenesis. Intriguingly, Del-1-deficient mice displayed increased neovascularisation in two independent ischaemic models (retinopathy of prematurity and hind-limb ischaemia), as compared to Del-1-proficient mice...
April 27, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28443045/automatic-approach-tendencies-toward-high-and-low-caloric-food-in-restrained-eaters-influence-of-task-relevance-and-mood
#6
Renate A M Neimeijer, Anne Roefs, Brian D Ostafin, Peter J de Jong
Objective: Although restrained eaters are motivated to control their weight by dieting, they are often unsuccessful in these attempts. Dual process models emphasize the importance of differentiating between controlled and automatic tendencies to approach food. This study investigated the hypothesis that heightened automatic approach tendencies in restrained eaters would be especially prominent in contexts where food is irrelevant for their current tasks. Additionally, we examined the influence of mood on the automatic tendency to approach food as a function of dietary restraint...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28440504/adipor1-mediated-mir-3908-inhibits-glioblastoma-tumorigenicity-through-downregulation-of-stat2-associated-with-the-ampk-sirt1-pathway
#7
Xiangming Liu, Jinglong Chen, Jinqian Zhang
A prospective method of treatment for cancer is to inhibit oncogene signaling pathways with microRNA (miRNA or miR). In the present study, whether the expression of STAT2, AdipoR1/AMPK/SIRT1 pathway of glioma is regulated by miR-3908 was explored. To confirm whether the predicted miR-3908 is matched with STAT2 and AdipoR1, 3'UTR luciferase activity of STAT2 and AdipoR1 was assessed. In the presence of the mimics or inhibitors of miR-3908, cell function of glioma cells, such as proliferation, growth, migration, invasion and apoptosis were analyzed...
April 20, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28440026/mir-148b-inhibits-glycolysis-in-gastric-cancer-through-targeting-slc2a1
#8
Xiangfu Ding, Jingjing Liu, Tianzhou Liu, Zhiming Ma, Dacheng Wen, Jiaming Zhu
Although the molecular biology of GC has been well characterized, early diagnostic biomarkers and effective therapeutic options in gastric cancer are still under investigation. Here, we found that miR-148b expression decreased in human gastric cancer tissues compared with matched adjacent nontumor tissues by q-PCR analysis and in situ hybridization. Further investigation revealed that overexpression of miR-148b limited glycolysis including glucose consumption, lactate production in gastric cancer cell lines BGC-823 and MKN45...
April 24, 2017: Cancer Medicine
https://www.readbyqxmd.com/read/28439897/platelet-lysate-obtained-via-plateletpheresis-performed-in-standing-and-awake-equine-donors
#9
Scarlett M Sumner, Maria C Naskou, Merrilee Thoresen, Ian Copland, John F Peroni
BACKGROUND: Platelet preparations containing growth factors, attachment factors, and enzymes are appealing to enhance healing of injured tissues and as an alternative to xenogenic serum in cell culture media. Plateletpheresis is commonly used to collect platelets in human medicine but has not been validated in horses. STUDY DESIGN AND METHODS: Plateletpheresis to collect platelet concentrate was performed on six female, mixed breed, chemically restrained horses using commercially available apheresis equipment...
April 25, 2017: Transfusion
https://www.readbyqxmd.com/read/28439064/immunotherapy-of-dual-function-vector-with-both-immunostimulatory-and-b-cell-lymphoma-2-bcl-2-silencing-effects-on-gastric-carcinoma
#10
Lanying Ma, Mei Han, Zumureti Keyoumu, Hua Wang, Saifuding Keyoumu
BACKGROUND Tumorigenesis is a kind of pathology marked by infinite proliferation and restrained apoptosis compared with normal cells. The abnormal expression of some proto-oncogenes and apoptosis inhibition are essential for tumor growth, which has been confirmed by molecular biologic and immunologic studies. The hypofunction of the host immune system also drives the development and metastasis of malignant tumors. Bcl-2, which has a critical role in regulating apoptosis, is overexpressed in several cancers...
April 25, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28437863/sirt3-acts-as-a-negative-regulator-of-autophagy-dictating-hepatocyte-susceptibility-to-lipotoxicity
#11
Songtao Li, Xiaobing Dou, Hua Ning, Qing Song, Wei Wei, Ximei Zhang, Chen Shen, Jiaxin Li, Changhao Sun, Zhenyuan Song
Lipotoxicity induced by saturated fatty acids (SFAs) plays a central role in the pathogenesis of non-alcoholic fatty liver disease (NAFLD); however, the exact mechanism(s) remain to be fully elucidated. SIRT3 is an NAD(+) -dependent deacetylase primarily located inside mitochondria. In this study, we demonstrated that a SFAs-rich high-fat diet (HFD) was more detrimental to the liver than an isocaloric unsaturated FAs-rich HFD. Unexpectedly, SIRT3 expression/activity were significantly elevated in the livers of mice exposed to the SFAs-rich HFD...
April 24, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28437772/shifts-in-soil-microbial-metabolic-activities-and-community-structures-along-a-salinity-gradient-of-irrigation-water-in-a-typical-arid-region-of-china
#12
Lijuan Chen, Changsheng Li, Qi Feng, Yongping Wei, Hang Zheng, Yan Zhao, Yongjiu Feng, Huiya Li
Saline water irrigation can change soil environment, which thereby influence soil microbial process. Based on a field experiment, the shifts in soil microbial metabolic activities and community structures under five irrigation salinities were studied using Biolog and metagenomic methods in this study. The results demonstrated that microbial metabolic activities were greatly restrained in saline water irrigated soils, as average well color development (AWCD) reduced under all saline water irrigation treatments...
April 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28436000/foxo3a-suppression-and-vps34-activity-are-essential-to-anti-atrophic-effects-of-leucine-in-skeletal-muscle
#13
Igor L Baptista, João G Silvestre, William J Silva, Siegfried Labeit, Anselmo S Moriscot
Our aim is to gain insight into the mechanisms underlying the anti-atrophic effects of leucine, namely, the way that this amino acid can restrain the up-regulation of MuRF1 and Mafbx/Atrogin-1 in muscle atrophy. Male rats received dietary leucine supplementation for 1-3 days, during which time their hind limbs were immobilized. Our results showed that leucine inhibited Forkhead Box O3 (FoxO3a) translocation to cell nuclei. In addition, leucine was able to reverse the expected reduction of FoXO3a ubiquitination caused by immobilization...
April 24, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28429962/predictors-of-restraint-use-among-child-occupants
#14
Marco Benedetti, Kathleen D Klinich, Miriam A Manary, Carol A Flannagan
OBJECTIVES: Identify factors that predict restraint use and optimal restraint use among children aged 0 to 13 years. METHODS: The dataset is a national sample of police-reported crashes for years 2010-2014 in which type of child restraint is recorded. The dataset was supplemented with demographic census data linked by driver zip code, as well as a score for the state child restraint law during the year of the crash relative to best practice recommendations for protecting child occupants...
April 21, 2017: Traffic Injury Prevention
https://www.readbyqxmd.com/read/28429351/tgf%C3%AE-1-protects-myocardium-from-apoptosis-and-oxidative-damage-after-ischemia-reperfusion
#15
Y-F Liu, Y-Y Chu, X-Z Zhang, M Zhang, F-G Xie, M Zhou, H-H Wen, A-H Shu
OBJECTIVE: Myocardial apoptosis is an important pathologic basis of ischemia-reperfusion injury (I/R). Transforming growth factor β1 (TGFβ1) participates in the regulation of oxidative damage and apoptosis. TGFβ1 is upregulated in the repair process of I/R injury. It is speculated that TGFβ1 over-expression is involved in the endogenous protective mechanism of I/R injury. This study explores the significance of TGFβ1 in myocardial cell apoptosis after I/R. MATERIALS AND METHODS: Rat myocardial I/R injury model was established...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28428618/pulmonary-delivery-of-triptolide-loaded-liposomes-decorated-with-anti-carbonic-anhydrase-ix-antibody-for-lung-cancer-therapy
#16
Congcong Lin, Blenda Chi Kwan Wong, Hubiao Chen, Zhaoxiang Bian, Ge Zhang, Xue Zhang, Muhammad Kashif Riaz, Deependra Tyagi, Ge Lin, Yanbo Zhang, Jinjin Wang, Aiping Lu, Zhijun Yang
Antibody-decorated liposomes can facilitate the precise delivery of chemotherapeutic drugs to the lung by targeting a recognition factor present on the surface of lung tumor cells. Carbonic anhydrase IX (CA IX) is an enzyme expressed on the surface of lung cancer cells with a restricted expression in normal lungs. Here, we explored the utility of anti-carbonic anhydrase IX (CA IX) antibody, conjugated to the surface of triptolide (TPL)-loaded liposomes (CA IX-TPL-Lips), to promote the therapeutic effects for lung cancer via pulmonary administration...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28428575/emotional-relationships-between-child-patients-and-their-mothers-during-dental-treatments
#17
(no author information available yet)
But did the 'uncooperative child' demonstrate higher sympathetic nervous activity because they were bound in the restraining device?
April 21, 2017: British Dental Journal
https://www.readbyqxmd.com/read/28427953/booster-seat-effectiveness-among-older-children-evidence-from-washington-state
#18
D Mark Anderson, Lindsay L Carlson, Daniel I Rees
INTRODUCTION: The American Academy of Pediatrics has recommended that children as old as 12 years use a booster seat when riding in motor vehicles, yet little is known about booster seat effectiveness when used by older children. This study estimated the association between booster use and injuries among children aged 8-12 years who were involved in motor vehicle crashes. METHODS: Researchers analyzed data on all motor vehicle crashes involving children aged 8-12 years reported to the Washington State Department of Transportation from 2002 to 2015...
April 17, 2017: American Journal of Preventive Medicine
https://www.readbyqxmd.com/read/28427951/uncovering-the-immune-responses-of-apis-mellifera-ligustica-larval-gut-to-ascosphaera-apis-infection-utilizing-transcriptome-sequencing
#19
Dafu Chen, Rui Guo, Xijian Xu, Cuiling Xiong, Qin Liang, Yanzhen Zheng, Qun Luo, Zhaonan Zhang, Zhijian Huang, Dhiraj Kumar, Weijun Xi, Xuan Zou, Min Liu
Honeybees are susceptible to a variety of diseases, including chalkbrood, which is capable of causing huge losses of both the number of bees and colony productivity. This research is designed to characterize the transcriptome profiles of Ascosphaera apis-treated and un-treated larval guts of Apis mellifera ligustica in an attempt to unravel the molecular mechanism underlying the immune responses of western honeybee larval guts to mycosis. In this study, 24, 296 and 2157 genes were observed to be differentially expressed in A...
April 18, 2017: Gene
https://www.readbyqxmd.com/read/28427342/intermittent-parathyroid-hormone-pth-promotes-cementogenesis-and-alleviates-the-catabolic-effects-of-mechanical-strain-in-cementoblasts
#20
Yuyu Li, Zhiai Hu, Chenchen Zhou, Yang Xu, Li Huang, Xin Wang, Shujuan Zou
BACKGROUND: External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment...
April 20, 2017: BMC Cell Biology
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