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https://www.readbyqxmd.com/read/27891466/an-in-vitro-evaluation-of-retention-colonization-and-penetration-of-commonly-used-denture-lining-materials-by-candida-albicans
#1
Sushma Krishnamurthy, Rajendra B Hallikerimath
INTRODUCTION: Colonization of the surface by Candida albicans and related Candida species is one of the major concerns of denture lining materials. AIM: We evaluated retention, colonization and penetration of the four denture lining materials namely Molloplast B, Permaflex, GC Soft Liner and Ufi Gel Hard C by Candida albicans. MATERIAL AND METHODS: a) Evaluation of retention: Five test discs of each material with smooth surface on one side and rough on the other were prepared and surface roughness (Ra) was measured with profilometer...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27889166/taggiasca-extra-virgin-olive-oil-colonization-by-yeasts-during-the-extraction-process
#2
G Ciafardini, G Cioccia, B A Zullo
The opalescent appearance of the newly produced olive oil is due to the presence of solid particles and microdrops of vegetation water in which the microorganisms from the olives' carposphere are trapped. Present research has demonstrated that the microbiota of the fresh extracted olive oil, produced in the mills, is mainly composed of yeasts and to a lesser extent of molds. The close link between the composition of the microbiota of the olives' carposphere undergoing to processing, and that of the microbiota of the newly produced olive oil, concerns only the yeasts and molds, given that the bacterial component is by and large destroyed mainly in the kneaded paste during the malaxation process...
April 2017: Food Microbiology
https://www.readbyqxmd.com/read/27882253/candida-glabrata-pneumonia-in-a-patient-with-chronic-obstructive-pulmonary-disease
#3
Onur Yazici, Mustafa Cortuk, Hasan Casim, Erdogan Cetinkaya, Ali Mert, Ali Ramazan Benli
Pneumonia remains an important cause of morbidity and mortality among infectious diseases. Streptococcus pneumoniae and viruses are the most common cause of pneumonia. Candidiasis in such patients has been associated with haemodialysis, fungal colonization, exposure to broad-spectrum antibiotics, intensive care unit (ICU) hospitalization, and immunocompromised patients. The most common cause of infection is C. albicans. The case presented here is of a 66-year-old male patient diagnosed with C. glabrata. The patient suffered from chronic obstructive pulmonary disease...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27879329/phenotypic-profiling-reveals-that-candida-albicans-opaque-cells-represent-a-metabolically-specialized-cell-state-compared-to-default-white-cells
#4
Iuliana V Ene, Matthew B Lohse, Adrian V Vladu, Joachim Morschhäuser, Alexander D Johnson, Richard J Bennett
: The white-opaque switch is a bistable, epigenetic transition affecting multiple traits in Candida albicans including mating, immunogenicity, and niche specificity. To compare how the two cell states respond to external cues, we examined the fitness, phenotypic switching, and filamentation properties of white cells and opaque cells under 1,440 different conditions at 25°C and 37°C. We demonstrate that white and opaque cells display striking differences in their integration of metabolic and thermal cues, so that the two states exhibit optimal fitness under distinct conditions...
November 22, 2016: MBio
https://www.readbyqxmd.com/read/27874093/enterohemorrhagic-escherichia-coli-promotes-the-invasion-and-tissue-damage-of-enterocytes-infected-with-candida-albicans-in-vitro
#5
Weiming Yang, Yanjun Zhou, Chunrong Wu, Jianguo Tang
The principal aim of this study was to investigate the in vitro co-infection of Caco-2 cells with Candida albicans and enterohemorrhage Escherichia coli (EHEC). The ability of both species to colonize or invade the Caco-2 cells was evaluated by indirect immunofluorescence and inverted microscopy. The damage to Caco-2 cells was evaluated by measuring lactate dehydrogenase (LDH) activity. C. albicans virulence gene expression (HWP1, ALS3, PLB1, SAP4, and EFG1) was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR)...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27855078/echinocandin-resistance-in-candida-spp-isolated-from-liver-transplant-recipients
#6
Gwénolé Prigent, Nawel Aït-Ammar, Eric Levesque, Arnaud Fekkar, Jean-Marc Costa, Sarra El Anbassi, Françoise Foulet, Christophe Duvoux, Jean-Claude Merle, Eric Dannaoui, Françoise Botterel
Liver transplant recipients are at risk of invasive fungal infections, especially candidiasis. Echinocandin is recommended as prophylactic treatment but is increasingly associated with resistance. Our aim was to assess echinocandin drug resistance in Candida spp. isolated from liver transplant recipients treated with this antifungal class. For this, all liver-transplanted patients in a University Hospital (Créteil, France) between January and June of 2013 and 2015 were included. Susceptibilities of Candida isolates to echinocandins were tested by Etest® and EUCAST reference method...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27853453/gradual-changes-of-gut-microbiota-in-weaned-miniature-piglets
#7
Jun Hu, Yangfan Nie, Jianwei Chen, Yong Zhang, Zhichang Wang, Qiwen Fan, Xianghua Yan
Colonization of gut microbiota in mammals during the early life is vital to host health. The miniature piglet has recently been considered as an optimal infant model. However, less is known about the development of gut microbiota in miniature piglets. Here, this study was conducted to explore how the gut microbiota develops in weaned Congjiang miniature piglets. In contrast to the relatively stabilized gut fungal community, gut bacterial community showed a marked drop in alpha diversity, accompanied by significant alterations in taxonomic compositions...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27850307/668-impact-of-candida-colonization-to-predict-candidemia-in-intensive-care-unit-patients
#8
Daniel Kett, Roland Schein, Cynthia Cely, Luis Rosario, Sameer Bajaj, Rajan Garg, Andrew Quartin
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27850225/585-role-of-short-chain-fatty-acids-in-modulating-candida-albicans-gastrointestinal-tract-colonization
#9
Pallav Bhattarai, Laura Coughlin, Andrew Koh
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27830437/temporal-profile-of-biofilm-formation-gene-expression-and-virulence-analysis-in-candida-albicans-strains
#10
Patrícia Pimentel de Barros, Rodnei Dennis Rossoni, Felipe De Camargo Ribeiro, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge
The characterization of Candida albicans strains with different degrees of virulence became very useful to understand the mechanisms of fungal virulence. Then, the objective of this study was to assess and compare the temporal profiles of biofilms formation, gene expression of ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 and virulence in Galleria mellonella of C. albicans ATCC18804 and a clinical sample isolated from an HIV-positive patient (CA60). Although the CFU/mL counting was higher in biofilms formed in vitro by ATCC strain, the temporal profile of the analysis of the transcripts of the C...
November 9, 2016: Mycopathologia
https://www.readbyqxmd.com/read/27824006/white-opaque-switching-in-different-mating-type-like-locus-gene-types-of-clinical-candida-albicans-isolates
#11
Hou-Min Li, Yumi Shimizu-Imanishi, Reiko Tanaka, Ruo-Yu Li, Takashi Yaguchi
BACKGROUND: Candida albicans (C. albicans) can become a pathogen causing superficial as well as life-threatening systemic infections, especially in immunocompromised patients. Many phenotypic attributes contribute to its capacity to colonize human organs. In our study, 93 C. albicans isolates from patients of various candidiasis in a hospital of China were surveyed. We aimed to investigate the white-opaque (WO) switching competence, drug sensitivity, and virulence of mating type-like (MTL) a/α isolates...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27820691/bacterial-colonization-in-the-marginal-region-of-ceramic-restorations-effects-of-different-cement-removal-methods-and-polishing
#12
Smb Pereira, L C Anami, C A Pereira, Roa Souza, K Z Kantorski, M A Bottino, Aoc Jorge, L F Valandro
This study evaluated the effects of excess cement removal techniques, with or without subsequent polishing, on biofilm formation and micromorphology in the marginal region of the tooth/restoration. From bovine teeth, 96 dentin blocks (4 × 8 × 2 mm) were produced, molded, and reproduced in type IV gypsum, on which 96 pressed ceramic blocks (Vita PM9, Vita Zahnfabrik; 4 × 8 × 2 mm) were produced via the lost wax technique. The dentin blocks and their respective ceramic blocks were cemented with a self-adhesive resin cement (RelyX U200, 3M ESPE), and cement excess was removed from the margin using four different techniques, followed or not by polishing with silicone rubber tips: MBr, removal with microbrush and photoactivation; MBr-Pol, MBr + polishing; Br, removal with brush and photoactivation; Br-Pol, Br + polishing; Photo-Expl, 5 seconds of initial photoactivation, removal with explorer, and final curing; Photo-Expl-Pol, Photo-Expl + polishing; Photo-SB, 5 seconds of initial photoactivation, removal with scalpel, and final curing; and Photo-SB-Pol, Photo-SB + polishing...
November 2016: Operative Dentistry
https://www.readbyqxmd.com/read/27815348/the-cghaa1-regulon-mediates-response-and-tolerance-to-acetic-acid-stress-in-the-human-pathogen-candida-glabrata
#13
Ruben T Bernardo, Diana V Cunha, Can Wang, Leonel Pereira, Sónia Silva, Sara B Salazar, Markus S Schröder, Michiyo Okamoto, Azusa Takahashi-Nakaguchi, Hiroji Chibana, Toshihiro Aoyama, Isabel Sá-Correia, Joana Azeredo, Geraldine Butler, Nuno Pereira Mira
To thrive in the acidic vaginal tract C. glabrata has to cope with high concentrations of acetic acid. The mechanisms underlying C. glabrata tolerance to acetic acid at low pH remain largely uncharacterized. In this work it is demonstrated the essential role of the CgHaa1 transcription factor (encoded by ORF CAGL0L09339g) in the response and tolerance of C. glabrata to acetic acid. Transcriptomic analysis showed that CgHaa1 regulates, directly or indirectly, the expression of about 75% of the genes activated under acetic acid stress...
November 4, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27793494/probiotics-prevent-candida-colonization-and-invasive-fungal-sepsis-in-preterm-neonates-a-systematic-review-and-meta-analysis-of-randomized-controlled-trials
#14
REVIEW
Hua-Jian Hu, Guo-Qiang Zhang, Qiao Zhang, Shristi Shakya, Zhong-Yue Li
To investigate whether probiotic supplementation could reduce the risk of fungal infection in preterm neonates in neonatal intensive care units (NICUs), we systematically searched PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials databases for randomized controlled trials (RCTs) focusing on the effect of probiotics on fungal infection in preterm neonates. The outcomes of interest were Candida colonization and invasive fungal sepsis. Seven trials involving 1371 preterm neonates were included...
September 14, 2016: Pediatrics and Neonatology
https://www.readbyqxmd.com/read/27773854/fabrication-of-antimicrobial-silver-doped-carbon-structures-by-combinatorial-pulsed-laser-deposition
#15
Ion N Mihailescu, Dorota Bociaga, Gabriel Socol, George E Stan, Mariana-Carmen Chifiriuc, Coralia Bleotu, Marius A Husanu, Gianina Popescu-Pelin, Liviu Duta, Catalin R Luculescu, Irina Negut, Claudiu Hapenciuc, Cristina Besleaga, Irina Zgura, Florin Miculescu
We report on the selection by combinatorial pulsed laser deposition of Silver-doped Carbon structures with reliable physical-chemical characteristics and high efficiency against microbial biofilms. The investigation of the films was performed by scanning electron microscopy, high resolution atomic force microscopy, energy dispersive X-Ray Spectroscopy, X-ray diffraction, Raman spectroscopy, bonding strength "pull-out" tests, and surface energy measurements. In vitro biological assays were carried out using a large spectrum of bacterial and fungal strains, i...
October 20, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27770528/bacteriological-examination-of-the-abdominal-effusion-in-bacterial-peritonitis
#16
T Didbaridze, L Saginashvili, L Akhmeteli, B Iremashvili, N Gogokhia
This study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. Our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (API20E, API Staph, API Strep, API Ana, BioMerieux). Rapid tests for detection of oxidase and catalase activity were also used. Susceptibility of microorganisms to antibiotics was defined by the disc-diffusion method using standard discs (EUCAST guidelines 2015) according to Clinical Laboratory Standard Institute (CLSI) protocols (ATB strips: ATB G, ATB Staph, ATBANA, ATBPse, ATBStrep...
September 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27769740/epico-3-0-management-of-non-neutropenic-patients-in-medical-wards
#17
Ricardo Ferrer, Rafael Zaragoza, Alejandro H Rodríguez, Emilio Maseda, Pedro Llinares, Santiago Grau, Francisco Álvarez-Lerma, Guillermo Quindós, Miguel Salavert, Rafael Huarte
BACKGROUND: Although the management of invasive fungal infection (IFI) has improved, a number of controversies persist regarding the approach to invasive fungal infection in non-neutropenic medical ward patients. AIMS: To identify the essential clinical knowledge to elaborate a set of recommendations with a high level of consensus necessary for the management of IFI in non-neutropenic medical ward patients. METHODS: A prospective, Spanish questionnaire, which measures consensus through the Delphi technique, was anonymously answered and e-mailed by 30 multidisciplinary national experts, all specialists (intensivists, anesthesiologists, microbiologists, pharmacologists and specialists in infectious diseases) in IFI and belonging to six scientific national societies...
October 18, 2016: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/27756539/prevalence-of-candida-spp-in-cervical-vaginal-samples-and-the-in-vitro-susceptibility-of-isolates
#18
Tchana Martinez Brandolt, Gabriel Baracy Klafke, Carla Vitola Gonçalves, Laura Riffel Bitencourt, Ana Maria Barral de Martinez, Josiara Furtado Mendes, Mário Carlos Araújo Meireles, Melissa Orzechowski Xavier
Vulvovaginal candidiasis (VVC) is an infection of the genital mucosa caused by different species of the genus Candida. Considering the lack of data on this topic in the south of Brazil, this study aimed to assess the prevalence of Candida spp. in the cervical-vaginal mucosa of patients treated at a university hospital in southern Rio Grande do Sul, as well as the etiology and the susceptibility of the isolates against fluconazole, itraconazole, miconazole and nystatin. Samples were collected at the gynecology clinic of the Federal Hospital of the University of Rio Grande, and the isolates were identified using phenotypic and biochemical tests...
October 4, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27747631/real-time-pcr-analysis-of-fungal-organisms-and-bacterial-species-at-peri-implantitis-sites
#19
Frank Schwarz, Kathrin Becker, Sebastian Rahn, Andrea Hegewald, Klaus Pfeffer, Birgit Henrich
BACKGROUND: The potential role of fungal organisms and their co-aggregation with either periodontopathogens or opportunistic pathogens at peri-implantitis sites is unknown. The aim of the present study was to qualitatively/quantitatively analyze and correlate fungal organisms and bacterial species at peri-implantitis sites. METHODS: In a total of 29 patients, submucosal/subgingival plaque samples were collected at peri-implantitis and healthy implant sites as well as teeth with a history of periodontitis (controls)...
December 2015: Int J Implant Dent
https://www.readbyqxmd.com/read/27736307/fungi-in-the-healthy-human-gastrointestinal-tract
#20
Heather E Hallen-Adams, Mallory J Suhr
Many species of fungi have been detected in the healthy human gut; however, nearly half of all taxa reported have only been found in one sample or one study. Fungi capable of growing in and colonizing the gut are limited to a small number of species, mostly Candida yeasts and yeasts in the family Dipodascaceae (Galactomyces, Geotrichum, Saprochaete). Malassezia and the filamentous fungus Cladosporium are potential colonizers; more work is needed to clarify their role. Other commonly-detected fungi come from the diet or environment but either cannot or do not colonize (Penicillium and Debaryomyces species, which are common on fermented foods but cannot grow at human body temperature), while still others have dietary or environmental sources (Saccharomyces cerevisiae, a fermentation agent and sometime probiotic; Aspergillus species, ubiquitous molds) yet are likely to impact gut ecology...
October 13, 2016: Virulence
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