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congshan jiang

Jing Xu, Wenhua Zhu, Yuanxu Guo, Congshan Jiang, Nazim Hussain, Liesu Meng, Shemin Lu
The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescence-activated cell sorting (FACS). In the present work, PCR, restriction endonuclease digestion and molecular cloning techniques were used to insert the gene decoding green fluorescent protein (GFP) without changing the structure of original plasmid to extend its application...
October 11, 2016: Applied Biochemistry and Biotechnology
Shanshan Wu, Rui Zhang, Fayi Nie, Xiaoli Wang, Congshan Jiang, Meng Liu, Robert K Valenzuela, Wanqing Liu, Yongyong Shi, Jie Ma
MicroRNAs (miRNAs) are a class of endogenous and non-coding single-stranded RNAs of approximately 22 nucleotides, many of which are evolutionarily conserved. Genome-wide association studies have identified a robust statistical association between the MIR137 gene and schizophrenia in Europeans, which was replicated in the Han Chinese population in a case-control study. In the previous study, we provided evidence for a significant association between the EFNB2 gene and schizophrenia in Han Chinese subjects. In the current study, we utilized computational analysis, vector construction of point mutations, luciferase reporter assays and gene expression assays including RT-qPCR and western blotting methods to investigate miR-137 directly targeting EFNB2 gene and explore the reversal effect of a genetic variant of SNP rs550067317 in the putative seed-pair region of EFNB2 3'-UTR...
September 15, 2016: EBioMedicine
Jidong Yan, Jing Xu, Yao Fei, Congshan Jiang, Wenhua Zhu, Yan Han, Shemin Lu
Thioredoxin reductase 2 (TrxR2) is a selenium (Se) containing protein. Se deficiency is associated with an endemic osteoarthropathy characterized by impaired cartilage formation. It is unclear whether TrxR2 have roles in cartilage function. We examined the effects of TrxR2 on chondrogenic ATDC5 cells through shRNA-mediated gene silencing of TrxR2. We demonstrated TrxR2 deficiencies could enhance chondrogenic differentiation and apoptosis of ATDC5 cells. TrxR2 deficiencies increased accumulation of cartilage glycosaminoglycans (GAGs) and mineralization...
May 15, 2016: Experimental Cell Research
Congshan Jiang, Hongchuan Yu, Qingzhu Sun, Wenhua Zhu, Jing Xu, Ning Gao, Rui Zhang, Li Liu, Xiaoying Wu, Xudong Yang, Liesu Meng, Shemin Lu
BACKGROUND: This study aims to find out whether extracellular miRNAs is implicated in recurrent childhood wheezing with asthmatic risk. METHODS: One hundred and forty children of Chinese Han population were recruited for this study. Plasma and intracellular miRNAs from children with recurrent wheezing and rats with antigen induced pulmonary inflammation (AIPI) were detected by using reverse transcription-quantitative PCR. Differential leukocytes in blood were automatically counted...
2016: BMC Pulmonary Medicine
Qingzhu Sun, Li Liu, Michael Roth, Jia Tian, Qirui He, Bo Zhong, Ruanjuan Bao, Xi Lan, Congshan Jiang, Jian Sun, Xudong Yang, Shemin Lu
Protein arginine methyltransferase (PRMT)1, methylating both histones and key cellular proteins, has emerged as a key regulator of various cellular processes. This study aimed to identify the mechanism that regulates PRMT1 in chronic Ag-induced pulmonary inflammation (AIPI) in the E3 rat asthma model. E3 rats were challenged with OVA for 1 or 8 wk to induce acute or chronic AIPI. Expression of mRNAs was detected by real-time quantitative PCR. PRMT1, TGF-β, COX2, and vascular endothelial growth factor protein expression in lung tissues was determined by immunohistochemistry staining and Western blotting...
July 1, 2015: Journal of Immunology: Official Journal of the American Association of Immunologists
Zixin Min, Rui Zhang, Jianfeng Yao, Congshan Jiang, Yuanxu Guo, Fei Cong, Wei Wang, Jia Tian, Nannan Zhong, Jian Sun, Jie Ma, Shemin Lu
Osteoarthritis (OA) is characterized by degeneration of articular cartilage, limited intraarticular inflammation with synovitis, and changes in peri-articular and subchondral bone. In recent years, more and more evidence demonstrated that microRNAs (miRNAs) play important roles in the molecular mechanisms in OA by suppressing gene expression at the post-transcriptional level. In current study, histological staining of toluidine blue and cartilage-specific gene express revealed that the bone matrix gelatin (BMG) rat model could demonstrate the different development of cartilage...
2015: International Journal of Clinical and Experimental Medicine
Wenhua Zhu, Congshan Jiang, Jing Xu, Manman Geng, Xiaoying Wu, Jian Sun, Jie Ma, Rikard Holmdahl, Liesu Meng, Shemin Lu
Based on pristane-induced arthritis (PIA), we found that T cells mediate TLR3 overexpression in fibroblast-like synoviocytes (FLS). The aim of this study is to determine key factors by which T cells induce TLR3 expression. Rat FLS were co-cultured with pristane primed T cell conditioned medium (PPT medium), and TLR3 expression of FLS was significantly induced. TNF-α, IFN-γ and IL-17 were dominantly expressed in PIA T cells. The overexpression of TLR3 and its related genes in FLS co-cultured with PPT medium could be reduced through blocking TNF-α pathway...
February 2015: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Yan Zhou, Weikun Hou, Ke Xu, Dan Han, Congshan Jiang, Kuanhou Mou, Yue Li, Liesu Meng, Shemin Lu
OBJECTIVE: The objective was to survey the expression and localization of Th17-related cytokines and their correlation with skin lesion severity in early systemic sclerosis (SSc). METHODS: The mRNA expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR) from 21 SSc patients and 12 healthy controls (HC). The protein expression was examined by immunohistochemistry (IHC) and Western blotting. RESULTS: The RT-qPCR analysis showed a significantly higher expression of IL-17A, IL-21, IL-22, IL-26, IL-17RA, IL-21R, and IL-22R1 mRNA; consistently, the IHC analysis showed an over-expression of IL-17RA, IL-21R and IL-22R1 and the Western blotting analysis showed an over-expression of IL-17A, IL-21, IL-21R and IL-22R1 in early SSc skin lesions...
January 2015: Human Immunology
Yong Peng, Congshan Guo, Yanhong Yang, Fenglin Li, Yanxia Zhang, Bin Jiang, Qingwang Li
A number of studies have shown that baicalein shows high antitumor activity in vitro and in vivo. In this study, the inhibitory effect of baicalein on human cervical cancer HeLa cells was studied in vitro. HeLa cells were treated with high (100 µg/ml) and low (50 µg/ml) doses of baicalein, and cell growth inhibition rates were examined by the MTT assay. The morphological changes of apoptotic cells were observed under the light and electron microscope, while the rate of cell apoptosis was examined by flow cytometry...
March 2015: Molecular Medicine Reports
Bo Zhong, Xudong Yang, Qingzhu Sun, Li Liu, Xi Lan, Jia Tian, Qirui He, Wei Hou, Haiyan Liu, Congshan Jiang, Ning Gao, Shemin Lu
Pdcd4 has been known as a tumor-suppressor gene initially and is up-regulated during apoptosis. Surprisingly, we found that Pdcd4 was differentially expressed in the lung from E3 rats with AIPI, an animal model for asthma, but the precise role of Pdcd4 in AIPI still remained to be defined. In the present study, we first evaluated the expression of Pdcd4 in lung from control and AIPI rats with RT-qPCR, Western blot, and immunohistochemistry. Then, we investigated the effects of intervention of Pdcd4 on markers of macrophage alternative activation and airway remodeling...
December 2014: Journal of Leukocyte Biology
Congshan Jiang, Wenhua Zhu, Jing Xu, Bo Wang, Weikun Hou, Rui Zhang, Nannan Zhong, Qilan Ning, Yan Han, Hongchuan Yu, Jian Sun, Liesu Meng, Shemin Lu
INTRODUCTION: Abnormal toll-like receptor (TLR)3 signaling plays an indispensable role in pathogenesis of both experimental and human rheumatoid arthritis, and microRNAs (miRNAs) might orchestrate this signaling pathway. This study was performed to determine the relationship between miR-26a and TLR3 in rat macrophages and to observe effects of miR-26a mimic on pristane induced arthritis (PIA) in rats. METHODS: Dual luciferase reporter assay was used to validate the direct interaction between miR-26a (a candidate miRNA to target tlr3 mRNA) and tlr3 3'UTR...
2014: Arthritis Research & Therapy
Wenhua Zhu, Liesu Meng, Congshan Jiang, Weikun Hou, Jing Xu, Bo Wang, Shemin Lu
Toll-like receptors (TLRs) have been found to contribute to the pathogenesis of rheumatoid arthritis (RA). The aim of this study is to investigate the regulation and potential role of TLR2 in spleen of pristane-induced arthritis (PIA) rat, which can be used to further understand the mechanisms of RA. Arthritis in DA rats was induced by pristane. TLR2 expression in spleen was detected by real-time quantitative PCR and western blotting, and TLR2 expression at both mRNA and protein levels was upregulated in PIA rats...
April 2012: Molecular Biology Reports
Wenhua Zhu, Liesu Meng, Congshan Jiang, Xiaojing He, Weikun Hou, Peng Xu, Heng Du, Rikard Holmdahl, Shemin Lu
INTRODUCTION: Toll-like receptors (TLRs) are likely to play crucial roles in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to determine the key TLRs in synovium and explore their roles in the activation of fibroblast-like synoviocytes (FLSs) mediated by T cells in arthritis. METHODS: Pristane-induced arthritis (PIA) was established by subcutaneous injection with pristane at the base of the rat's tail. TLR expression in synovium from PIA rats was detected at different time points by performing real-time PCR...
2011: Arthritis Research & Therapy
Liesu Meng, Xiaojing He, Wenhua Zhu, Xudong Yang, Congshan Jiang, Qingzhu Sun, M B, Simeng Zhang, Qian Xue, Xinfang Xie, Shemin Lu
BACKGROUND: Toll-like receptors (TLRs) as pattern recognition receptors, participate in both innate and adaptive immune responses, and seem to play an important role in the pathogenesis of asthma. This study aimed to identify key TLRs involved in antigen induced pulmonary inflammation (AIPI), a rat model for asthma, and to explore the role of TLRs in the disease development. METHODS AND FINDINGS: E3 rats were sensitized with ovalbumin (OVA)/alum intraperitoneally and intranasally challenged with OVA to induce AIPI model...
2011: PloS One
Liesu Meng, Wenhua Zhu, Congshan Jiang, Xiaojing He, Weikun Hou, Fang Zheng, Rikard Holmdahl, Shemin Lu
INTRODUCTION: Toll-like receptors (TLRs) are involved in both innate and adaptive immune responses and are likely to play a complex role in the pathogenesis of human rheumatoid arthritis (RA) and experimental arthritis. The objective of this study was to identify the key TLR in pristane-induced arthritis (PIA), a rat model for RA, and to clarify its roles in the initiation and maintenance of arthritis. METHODS: Arthritis in DA rats was induced by pristane and the severity was evaluated by macroscopic and microscopic score systems...
2010: Arthritis Research & Therapy
Luyun Yang, Yongjun Dong, Danping Chen, Chen Wang, Xiao Hu, Ning Da, Guangjun Zhao, Jun Xu, Xiongwei Jiang, Congshan Zhu, Jianrong Qiu
Infrared to ultraviolet and visible upconversion luminescence was demonstrated in trivalent cerium doped YAlO(3) crystal (Ce(3+): YAP) under focused infrared femtosecond laser irradiation. The fluorescence spectra show that the upconverted luminescence comes from the 5d-4f transitions of trivalent cerium ions. The dependence of luminescence intensity of trivalent cerium on infrared pumping power reveals that the conversion of infrared radiation is dominated by three-photon excitation process. It is suggested that the simultaneous absorption of three infrared photons pumps the Ce(3+) ion into upper 5d level, which quickly nonradiatively relax to lowest 5d level...
January 9, 2006: Optics Express
Congshan Jiang, Liesu Meng, Wenhua Zhu, Muhammad Shahzad, Xudong Yang, Shemin Lu
OBJECTIVE AND DESIGN: Aim of this study is to explore the expression stability of 12 commonly used housekeeping genes in inflammation condition. MATERIALS AND METHODS: Two rat models, pristane-induced arthritis (PIA) and antigen-induced pulmonary inflammation (AIPI) were established and reverse transcription-PCR was performed from rat spleens and inguinal lymph nodes. RESULTS: The reference gene expression fluctuated in the immune-associated tissues and 18S rRNA expression from the various inflammatory conditions was the most stably in all examined gene expression...
September 2009: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
Mingying Peng, Jianrong Qiu, Danping Chen, Xiangeng Meng, Ivyun Yang, Xiongwei Jiang, Congshan Zhu
Broadband infrared luminescence from bismuth-doped germanium oxide glasses prepared by a conventional melting-quenching technique was discovered. The absorption spectrum of the glasses covered a wide range from the visible to the near-infrared wavelength regions and consisted of five broad peaks below 370, 500, 700, 800, and 1000 nm. The fluorescence spectrum exhibited broadband characteristics (FWHM) greater than 300 nm with a maximum at 1300 nm pumped by an 808-nm laser. The fluorescence lifetime at room temperature decreased with increasing Bi2O3 concentration in the glass...
September 1, 2004: Optics Letters
Jianrong Qiu, Xiongwei Jiang, Congshan Zhu, Mitsuru Shirai, Jinhai Si, Nan Jiang, Kazuyuki Hirao
No abstract text is available yet for this article.
April 19, 2004: Angewandte Chemie
Jianrong Qiu, Xiongwei Jiang, Congshan Zhu, Hideyuki Inouye, Jinhai Si, Kazuyuki Hirao
We report on the optical properties of a structurally modified silicate glass doped with Au ions. The area in the vicinity of the focal point of an 800-nm femtosecond laser in a glass sample became gray as a result of the formation of color centers after laser irradiation and turned red because of precipitation of Au nanoparticles after further annealing at 550 degrees C for 30 min. When the glass was excited by UV light at 365 nm, yellowish-white and orange-yellow emissions were observed in the laser-irradiated and the Au-nanoparticle-precipitated area, respectively...
February 15, 2004: Optics Letters
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