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Proteomics IVF

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https://www.readbyqxmd.com/read/27924460/proteomic-profile-of-maternal-aged-blastocoel-fluid-suggests-a-novel-role-for-ubiquitin-system-in-blastocyst-quality
#1
Gabriella Tedeschi, Elena Albani, Elena Monica Borroni, Valentina Parini, Anna Maria Brucculeri, Elisa Maffioli, Armando Negri, Simona Nonnis, Mauro Maccarrone, Paolo Emanuele Levi-Setti
PURPOSE: The etiology of maternal aging, a common cause of female factor infertility and a rate-limiting step in vitro fertilization (IVF) success, remains still unclear. Proteomic changes responsible for the impaired successful pregnancy outcome after IVF with aged blastocysts have not been yet evaluated. The objective of this prospective study was to employ proteomic techniques and bioinformatic tools to enlight differences at the protein level in blastocoel fluid of aged and younger woman...
December 6, 2016: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/27670939/evaluation-of-spermatozoal-function-useful-tools-or-just-science
#2
W Niżański, A Partyka, S Prochowska
Conventional microscopic semen analysis does not provide precise information on the fertilizing potential of a male. The traditional basis for semen evaluation is that male fertility is dependent on production of a "proper" concentration/number of motile, morphologically normal spermatozoa in excess to achieve conception. Many independent studies, especially in human medicine, have demonstrated that the absolute number of spermatozoa does not accurately determine fertility, but their functional competence. Many functional tests of spermatozoa are developed over the last decades...
September 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27645816/omics-in-reproductive-medicine-application-of-novel-technologies-to-improve-the-ivf-success-rate
#3
R D Nerenz
Treatment for many infertile couples often consists of in vitro fertilization (IVF) but an estimated 70% of IVF cycles fail to produce a live birth. In an attempt to improve the live birth rate, the vast majority of IVF cycles performed in the United States involve the transfer of multiple embryos, a practice that increases the risk of multiple gestation pregnancy. This is a concern because multiple gestation pregnancies are associated with an increased incidence of maternal and fetal complications and significant cost associated with the care of preterm infants...
2016: Advances in Clinical Chemistry
https://www.readbyqxmd.com/read/27604460/improved-cryotolerance-and-developmental-potential-of-in-vitro-and-in-vivo-matured-mouse-oocytes-by-supplementing-with-a-glutathione-donor-prior-to-vitrification
#4
Tom Trapphoff, Martyna Heiligentag, Jenny Simon, Nora Staubach, Thorsten Seidel, Kathrin Otte, Thomas Fröhlich, Georg J Arnold, Ursula Eichenlaub-Ritter
STUDY QUESTION: Can supplementation of media with a glutathione (GSH) donor, glutathione ethyl ester (GEE), prior to vitrification protect the mouse oocyte from oxidative damage and critical changes in redox homeostasis, and thereby improve cryotolerance? SUMMARY ANSWER: GEE supplementation supported redox regulation, rapid recovery of spindle and chromosome alignment after vitrification/warming and improved preimplantation development of mouse metaphase II (MII) oocytes...
September 7, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/26895700/characteristics-of-follicular-fluid-in-ovaries-with-endometriomas
#5
Elisa Giacomini, Ana M Sanchez, Veronica Sarais, Soha Al Beitawi, Massimo Candiani, Paola Viganò
The study of follicular fluid (FF) content nearby endometriomas may assist in elucidating pathophysiology, possible biomarkers related to this disease and the effect of endometriomas on ovarian physiology. As the question "how endometrioma may intrude the physiology of ovarian tissue?" is still open, we aimed to summarize the molecular evidence supporting the idea that endometriomas can negatively influence the content of the surrounding ovarian follicles. An alteration of the iron metabolism and an increased ROS (reactive oxygen species) generation characterize the intrafollicular environment adjacent to endometriomas...
February 8, 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/26676989/heat-shock-protein-member-a2-forms-a-stable-complex-with-angiotensin-converting-enzyme-and-protein-disulfide-isomerase-a6-in-human-spermatozoa
#6
Elizabeth G Bromfield, Eileen A McLaughlin, Robert John Aitken, Brett Nixon
STUDY HYPOTHESIS: Given the importance of the chaperone Heat Shock Protein A2 (HSPA2) in the regulation of male fertility, this study aimed to identify and characterize additional proteins that may rely on the activity of this chaperone in human spermatozoa. STUDY FINDING: In view of the findings in this study we propose that angiotensin converting enzyme (ACE) and protein disulfide isomerase A6 (PDIA6) are novel interacting proteins of HSPA2 and that this multimeric complex may participate in key elements of the fertilization cascade...
February 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/26471863/characterization-and-quantification-of-proteins-secreted-by-single-human-embryos-prior-to-implantation
#7
Maurizio Poli, Alessandro Ori, Tim Child, Souraya Jaroudi, Katharina Spath, Martin Beck, Dagan Wells
The use of in vitro fertilization (IVF) has revolutionized the treatment of infertility and is now responsible for 1-5% of all births in industrialized countries. During IVF, it is typical for patients to generate multiple embryos. However, only a small proportion of them possess the genetic and metabolic requirements needed in order to produce a healthy pregnancy. The identification of the embryo with the greatest developmental capacity represents a major challenge for fertility clinics. Current methods for the assessment of embryo competence are proven inefficient, and the inadvertent transfer of non-viable embryos is the principal reason why most IVF treatments (approximately two-thirds) end in failure...
November 2015: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/26380864/non-invasive-assessment-of-in-vitro-embryo-quality-to-improve-transfer-success
#8
REVIEW
Tina Rødgaard, Peter M H Heegaard, Henrik Callesen
Although IVF has been performed routinely for many years to help couples with fertility problems and in relation to modern breeding of farm animals, pregnancy rates after transfer to a recipient have not improved during the last decade. Early prediction of the viability of in-vitro developed embryos before the transfer to a recipient still remains challenging. Presently, the predominant non-invasive technique for selecting viable embryos is based on morphology, where parameters such as rates of cleavage and blastocyst formation as well as developmental kinetics are evaluated mostly subjectively...
November 2015: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/26155198/discovery-of-porcine-maternal-factors-related-to-nuclear-reprogramming-and-early-embryo-development-by-proteomic-analysis
#9
Qi Zhao, Zheng Guo, Shanhua Piao, Chunsheng Wang, Tiezhu An
BACKGROUND: Differentiated cell nuclei can be reprogrammed to a pluripotent state in several ways, including incubation with oocyte extracts, transfer into enucleated oocytes, and induced pluripotent stem cell technology. Nuclear transfer-mediated reprogramming has been proven to be the most efficient method. Maternal factors stored in oocytes have critical roles on nuclear reprogramming and early embryo development, but remain elusive. RESULTS: In this study, we showed most of porcine oocytes became nuclear matured at 33 h of IVM and the rate had no significant difference with oocytes at 42 h of IVM (p > 0...
2015: Proteome Science
https://www.readbyqxmd.com/read/26114977/follicular-fluid-alterations-in-endometriosis-label-free-proteomics-by-ms-e-as-a-functional-tool-for-endometriosis
#10
Thais Regiani, Fernanda Bertuccez Cordeiro, Lívia do Vale Teixeira da Costa, Jéssica Salgueiro, Karina Cardozo, Valdemir Melechco Carvalho, Kayla Jane Perkel, Daniel Suslik Zylbersztejn, Agnaldo Pereira Cedenho, Edson Guimarães Lo Turco
Endometriosis is a chronic gynecological condition that affects 10-32% of women of reproductive age and may lead to infertility. The study of protein profiles in follicular fluid may assist in elucidating possible biomarkers related to this disease. For this, follicular fluid samples were obtained from women with tubal factor or minimal male factor infertility who had pregnancy outcomes after in vitro fertilization (IVF) treatment (control group, n = 10), women with endometriosis (endometriosis group, n = 10), along with the endometrioma from these same patients were included (endometrioma group, n = 10)...
2015: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/26098221/from-global-proteome-profiling-to-single-targeted-molecules-of-follicular-fluid-and-oocyte-contribution-to-embryo-development-and-ivf-outcome
#11
REVIEW
Moncef Benkhalifa, Aicha Madkour, Noureddine Louanjli, Nouzha Bouamoud, Brahim Saadani, Ismail Kaarouch, Hikmat Chahine, Omar Sefrioui, Philippe Merviel, Henri Copin
The development of in vitro fertilization (IVF) techniques for infertility management has led to the investigation of the proteome of follicular fluid and oocyte. In addition, different markers contributing to oocyte maturation and embryo development potential have been reported in the literature. Different techniques were utilized to analyze whole proteome or single protein markers in follicular fluid and oocytes, particularly in animal models. Data from several studies have generated large amounts of information, however, an ideal profile to predict the best oocytes and embryos suitable for implantation are still to be uncovered...
August 2015: Expert Review of Proteomics
https://www.readbyqxmd.com/read/26094490/preconception-folic-acid-use-influences-the-follicle-fluid-proteome
#12
John M Twigt, Karel Bezstarosti, Jeroen Demmers, Jan Lindemans, Joop S E Laven, Régine P Steegers-Theunissen
BACKGROUND: The investigation of the human follicle fluid proteome has gained much interest in the search of new markers as predictors for in vitro fertilization and intracytoplasmic sperm injection (IVF/ICSI) treatment outcome. Follicular fluid folate, as substrate of one carbon (1-C) metabolism, affects follicular metabolism and oocyte and embryo quality. From this background, we aim to identify a folate-related follicle fluid proteome that associates with IVF/ICSI treatment outcome...
August 2015: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/25935493/day-3-embryo-metabolomics-in-the-spent-culture-media-is-altered-in-obese-women-undergoing-in-vitro-fertilization
#13
José Bellver, María J De Los Santos, Pilar Alamá, Damià Castelló, Laura Privitera, Daniela Galliano, Elena Labarta, Carmen Vidal, Antonio Pellicer, Francisco Domínguez
OBJECTIVE: To determine whether the global metabolomic profile of the spent culture media (SCM) of day-3 embryos is different in obese and normoweight women undergoing in vitro fertilization (IVF). DESIGN: Prospective cohort analysis. SETTING: IVF clinic. PATIENT(S): Twenty-eight young, nonsmoking women with normoweight, nonsmoking male partners with mild/normal sperm factors undergoing a first IVF attempt for idiopathic infertility, tubal factor infertility, or failed ovulation induction: obese ovulatory women (n = 12); obese women with polycystic ovary syndrome (PCOS; n = 4); normoweight ovulatory women (n = 12)...
June 2015: Fertility and Sterility
https://www.readbyqxmd.com/read/25865850/the-role-of-the-molecular-chaperone-heat-shock-protein-a2-hspa2-in-regulating-human-sperm-egg-recognition
#14
REVIEW
Brett Nixon, Elizabeth G Bromfield, Matthew D Dun, Kate A Redgrove, Eileen A McLaughlin, R John Aitken
One of the most common lesions present in the spermatozoa of human infertility patients is an idiopathic failure of sperm-egg recognition. Although this unique cellular interaction can now be readily by-passed by assisted reproductive strategies such as intracytoplasmic sperm injection (ICSI), recent large-scale epidemiological studies have encouraged the cautious use of this technology and highlighted the need for further research into the mechanisms responsible for defective sperm-egg recognition. Previous work in this field has established that the sperm domains responsible for oocyte interaction are formed during spermatogenesis prior to being dynamically modified during epididymal maturation and capacitation in female reproductive tract...
July 2015: Asian Journal of Andrology
https://www.readbyqxmd.com/read/25595538/preliminary-proteomic-analysis-on-the-alterations-in-follicular-fluid-proteins-from-women-undergoing-natural-cycles-or-controlled-ovarian-hyperstimulation
#15
Yan-Ting Wu, Yan Wu, Jun-Yu Zhang, Ning-Ning Hou, Ai-Xia Liu, Jie-Xue Pan, Jie-Yang Lu, Jian-Zhong Sheng, He-Feng Huang
PURPOSE: To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. METHODS: Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes...
March 2015: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/25567750/diagnosis-of-human-preimplantation-embryo-viability
#16
REVIEW
David K Gardner, Marcos Meseguer, Carmen Rubio, Nathan R Treff
BACKGROUND: Transfer of more than a single embryo in an IVF cycle comes with the finite possibility of a multiple gestation. Even a twin pregnancy confers significant risk to both mother and babies. The move to single-embryo transfer for all patients will be greatly facilitated by the ability to quantify embryo viability. Developments in time-lapse incubation systems have provided new insights into the developmental kinetics of the human preimplantation embryo. Advances in molecular methods of chromosomal analysis have created platforms for highly effective screening of biopsied embryos, while noninvasive analysis of embryo physiology reveals more about the embryo than can be determined by morphology alone...
November 2015: Human Reproduction Update
https://www.readbyqxmd.com/read/25355644/investigation-of-male-infertility-using-quantitative-comparative-proteomics
#17
COMPARATIVE STUDY
Christine Légaré, Arnaud Droit, Frédéric Fournier, Sylvie Bourassa, André Force, Francine Cloutier, Roland Tremblay, Robert Sullivan
Male factors account for 40% of infertility cases. The identification of differentially expressed proteins on spermatozoa from fertile and infertile men can help in the elucidation of the molecular basis of male infertility. The aim of this study was to compare sperm proteomes from 3 different groups: fertile men, normozoospermic men consulting for infertility, and normozoospermic men with an impaired capacity for fertilization (IVF-failure). We used differential proteomics with isobaric tags for relative and absolute quantitation (iTRAQ) labeling, and LC-MS analysis to identify proteins that are differentially expressed...
December 5, 2014: Journal of Proteome Research
https://www.readbyqxmd.com/read/25164020/unconditioned-commercial-embryo-culture-media-contain-a-large-variety-of-non-declared-proteins-a-comprehensive-proteomics-analysis
#18
Thomas F Dyrlund, Kirstine Kirkegaard, Ebbe Toftgaard Poulsen, Kristian W Sanggaard, Johnny J Hindkjær, Jørgen Kjems, Jan J Enghild, Hans Jakob Ingerslev
STUDY QUESTION: Which non-declared proteins (proteins not listed on the composition list of the product data sheet) are present in unconditioned commercial embryo culture media? SUMMARY ANSWER: A total of 110 non-declared proteins were identified in unconditioned media and between 6 and 8 of these were quantifiable and therefore represent the majority of the total protein in the media samples. WHAT IS KNOWN ALREADY: There are no data in the literature on what non-declared proteins are present in unconditioned (fresh media in which no embryos have been cultured) commercial embryo media...
November 2014: Human Reproduction
https://www.readbyqxmd.com/read/25106620/deciphering-the-proteomic-signature-of-human-endometrial-receptivity
#19
Tamara Garrido-Gómez, Alicia Quiñonero, Oreto Antúnez, Patricia Díaz-Gimeno, Jose Bellver, Carlos Simón, Francisco Domínguez
STUDY QUESTION: Are there any proteomic differences between receptive (R) and non-receptive (NR) endometrial receptivity array (ERA)-diagnosed endometria obtained on the same day of a hormonal replacement therapy (HRT) treatment cycle? SUMMARY ANSWER: There is a different proteomic signature between R and NR ERA-diagnosed endometrium obtained on the same day of HRT cycles. WHAT IS KNOWN ALREADY: The human endometrial transcriptome has been extensively investigated in the last decade resulting in the development of a new diagnostic test based on the transcriptomic signature of the window of implantation (WOI)...
September 2014: Human Reproduction
https://www.readbyqxmd.com/read/25100710/altered-protein-expression-profiles-in-umbilical-veins-insights-into-vascular-dysfunctions-of-the-children-born-after-in-vitro-fertilization
#20
Qian Gao, Hai-Tao Pan, Xian-Hua Lin, Jun-Yu Zhang, Ying Jiang, Shen Tian, Lu-Ting Chen, Miao-E Liu, Yi-Meng Xiong, He-Feng Huang, Jian-Zhong Sheng
Cardiovascular dysfunction and remodeling have been found in some children conceived by in vitro fertilization (IVF). However, the underlying mechanisms remain unclear. In this study, the retrospective investigation showed that the blood pressure of IVF-conceived Chinese children was higher than that of naturally conceived (NC) children at ages 3-13 yr. We analyzed the expression profile of proteins in the umbilical veins of IVF and NC newborns by proteomic techniques. Using iTRAQ (isobaric tags for relative and absolute quantitation), 47 differentially expressed proteins (DEPs) were identified by feature selection in IVF umbilical veins compared with NC...
September 2014: Biology of Reproduction
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