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Hadeesha Piyadasa, Anthony Altieri, Sujata Basu, Jacquie Schwartz, Andrew J Halayko, Neeloffer Mookherjee
House dust mite (HDM) challenge is commonly used in murine models of allergic asthma for preclinical pathophysiological studies. However, few studies define objective readouts or biomarkers in this model. In this study we characterized immune responses and defined molecular markers that are specifically altered after HDM challenge. In this murine model, we used repeated HDM challenge for two weeks which induced hallmarks of allergic asthma seen in humans, including airway hyper-responsiveness (AHR) and elevated levels of circulating total and HDM-specific IgE and IgG1...
2016: Biology Open
(no author information available yet)
No abstract text is available yet for this article.
July 2015: Inflammatory Bowel Diseases
Robert V Lumsden, Julie C Worrell, Denise Boylan, Sinead M Walsh, Jennifer Cramton, Ian Counihan, Sarah O'Beirne, Maria Fe Medina, Jack Gauldie, Aurelie Fabre, Seamas C Donnelly, Rosemary Kane, Michael P Keane
Pulmonary fibrosis is a progressive and fatal disease that involves the remodeling of the distal airspace and the lung parenchyma, which results in compromised gas exchange. The median survival time once diagnosed is less than three years. Interleukin (IL)-13 has been shown to play a role in a number of inflammatory and fibrotic diseases. IL-13 modulates its effector functions via a complex receptor system that includes the IL-4 receptor (R) α, IL-13Rα1, and the IL-13Rα2. IL-13Rα1 binds IL-13 with low affinity, yet, when it forms a complex with IL-4α, it binds with much higher affinity, inducing the effector functions of IL-13...
April 1, 2015: American Journal of Physiology. Lung Cellular and Molecular Physiology
Ling-Yuan Kong, Jun Wei, Ali S Haider, Brandon D Liebelt, Xiaoyang Ling, Charles A Conrad, Gregory N Fuller, Nicholas B Levine, Waldemar Priebe, Raymond Sawaya, Amy B Heimberger
Subependymomas are usually treated with surgical resection; however, no standard, defined alternative medical therapy is recommended for patients who are not surgical candidates, owing to a paucity of molecular, immunological, and genetic characterization. To address this, an ex vivo functional analysis of the immune microenvironment in subependymoma was conducted, a subependymoma cytokine/chemokine microarray was constructed for the evaluation of operational immune and molecular pathways, and a subependymoma cell line was derived and used to test a variety of cytotoxic agents that target operational pathways identified in subependymoma...
December 15, 2014: Journal of Neuroimmunology
Yasuto Akiyama, Masaru Komiyama, Haruo Miyata, Mika Yagoto, Tadashi Ashizawa, Akira Iizuka, Chie Oshita, Akiko Kume, Masahiro Nogami, Ichiro Ito, Reiko Watanabe, Takashi Sugino, Koichi Mitsuya, Nakamasa Hayashi, Yoko Nakasu, Ken Yamaguchi
Glioblastoma multiforme (GBM) is one of the most malignant and aggressive tumors, and has a very poor prognosis with a mean survival time of <2 years, despite intensive treatment using chemo-radiation. Therefore, novel therapeutic approaches including immunotherapy have been developed against GBM. For the purpose of identifying novel target antigens contributing to GBM treatment, we developed 17 primary glioma cell lines derived from high-grade glioma patients, and analyzed the expression of various tumor antigens and glioma-associated markers using a quantitative PCR and immunohistochemistry (IHC)...
April 2014: Oncology Reports
Waldemar Debinski, Peter Dickinson, John H Rossmeisl, John Robertson, Denise M Gibo
Interleukin 13 receptor alpha 2 (IL-13RA2) is over-expressed in a vast majority of human patients with high-grade astrocytomas like glioblastoma. Spontaneous astrocytomas in dogs resemble human disease and have been proposed as translational model system for investigation of novel therapeutic strategies for brain tumors. We have generated reagents for both detection and therapeutic targeting of IL-13RA2 in human and canine brain tumors. Peptides from three different regions of IL-13RA2 with 100% sequence identity between human and canine receptors were used as immunogens for generation of monoclonal antibodies...
2013: PloS One
Joseph C Mazzei, Hui Zhou, Bradley P Brayfield, Raquel Hontecillas, Josep Bassaganya-Riera, Eva M Schmelz
Inflammation of the gastrointestinal tract increases the risk of developing colon cancer especially in younger adults. Dietary compounds are not only associated with the etiology of inflammation and colon cancer but also in their prevention. Sphingolipid metabolites have been shown to play a role in the initiation and perpetuation of inflammatory responses. In the present study, we investigated the suppression of dextran sodium sulfate-induced colitis and azoxymethane-induced colon cancer by dietary sphingomyelin (SM) in mice that lack functional peroxisome proliferator-activated receptor γ (PPAR-γ) in intestinal epithelial and immune cells...
December 2011: Journal of Nutritional Biochemistry
Mara J Broadhurst, Jacqueline M Leung, Vikram Kashyap, Joseph M McCune, Uma Mahadevan, James H McKerrow, P'ng Loke
Ulcerative colitis, a type of inflammatory bowel disease, is less common in countries endemic for helminth infections, suggesting that helminth colonization may have the potential to regulate intestinal inflammation in inflammatory bowel diseases. Indeed, therapeutic effects of experimental helminth infection have been reported in both animal models and clinical trials. Here, we provide a comprehensive cellular and molecular portrait of dynamic changes in the intestinal mucosa of an individual who infected himself with Trichuris trichiura to treat his symptoms of ulcerative colitis...
December 1, 2010: Science Translational Medicine
Anhua Wu, Katya Ericson, Wang Chao, Walter C Low
BACKGROUND: IL-13Ra2 is overexpressed by gliomas but not by normal tissue. However, the molecular basis for IL-13Ra2 overexpression in gliomas is unknown. METHODS: In the present study we have investigated the regulatory mechanisms that are responsible for the expression of IL-13Ra2 with mutation analysis, quantitative RT-PCR, Flow cytometry analysis, transcription factor binding assay and Elisa. RESULTS: Our results reveal a complex mechanism for regulating IL-13Ra2 expression that involves at least 2 promoters and 4 transcripts of human IL-13Ra2...
2010: Cellular Oncology: the Official Journal of the International Society for Cellular Oncology
Xinjiang Yan, Zhipeng Su, Jing Zhang, Zhebao Wu, Sheng Ye, Xianghe Lu, Jinsen Wu, Yanjun Zeng, Weiming Zheng
Recent studies show that IL-13Ralpha2, a brain tumor-associated antigen for IL-13, may play a role in immunotherapy for glioblastoma. Thus, we stimulated the lymphocyte by monocyte-derived dendritic cells (DCs). The DCs were pulsed with IL-13Ralpha2 in vitro and then co-cultured with lymphocytes. After inducing cytotoxic T cells (CTLs) and co-culturing with U251 cells for 24 h in 96 wells, Cell Count Kit-8 (CCK-8) was added to every well equally. The optical density (OD) value was detected and recorded after 2 h...
2010: Cellular Immunology
Wenqiao Yu, Zhipeng Su, Zhebao Wu, Xiaochun Mao, Weiming Zheng, Yanjun Zeng
Despite advances in surgical technology and radiation therapy, the prognosis in the patients with malignant glioma remains poor. Recent studies show that interleukin-13 receptor [alpha]2 chain (IL-13Ra2), a brain tumor-associated receptor for IL-13, may play a role in immunotherapy for glioblastoma. We thus amplified human IL-13Ra2 gene from the human glioblastoma cell line using RT-PCR and cloned the target gene into the pET-28a, a prokaryotic expressing plasmid. After transformation, the recombinant plasmid expressed a soluble protein induced by IPTG...
March 2009: Cancer Immunology, Immunotherapy: CII
Takeshi Shimamura, Syed R Husain, Raj K Puri
Targeting cell surface receptors with cytotoxins or immunotoxins provides a unique opportunity for brain tumor therapy. The authors have discovered that receptors for two cytokines, interleukin (IL)-4 and IL-13, are overexpressed on tumor biopsy samples and on cell lines derived from a variety of human tumors, including brain tumors. These investigators have demonstrated that the structure of these cytokine receptors on tumor cells is different from that found on normal immune cells. In human solid tumor cells, IL-4 binds to two chains (IL-4Ra and IL-13Ra1), whereas IL- 13 binds to three chains in many solid tumor cells, including glioma cells (to IL-4Ra, IL-13Ra1, and IL-13Ra2)...
2006: Neurosurgical Focus
Bharat H Joshi, Pamela Leland, Raj K Puri
AIM: To identify and characterize the subunit structure of interleukin-13 receptor (IL-13R) in human medulloblastoma cell lines and target them with a chimeric fusion protein consisting of interleukin-13 and Pseudomonas exotoxin (termed as IL-13 cytotoxin). METHODS: Five human medulloblastoma cell lines were examined for the expression of IL-13R subunits at the mRNA and protein levels by reverse transcriptase-polymerase chain reaction (RT-PCR) and indirect immunofluorescence studies, respectively...
August 2003: Croatian Medical Journal
An-Hua Wu, Walter C Low
The interleukin 13 alpha 2 receptor (IL-13Ra2) has been shown to be expressed in most malignant glioblastoma cells. Recent studies suggest that IL-13Ra2 serves as a dominant negative inhibitor or a decoy receptor for IL-13. To investigate the transcriptional regulation of this receptor, we cloned and characterized the promoter for the human IL-13Ra2 gene. Our results demonstrate that this promoter contains three TATA boxes and one CCAAT site. Several putative transcriptional factor binding sites for nuclear factor of activated T cells 1, AP1 (c-JUN and c-FOS), AP2, GABP, OCT1, GATA3, PRE, and C-ETS1 were predicted in the promoter region...
July 2003: Neuro-oncology
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