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Usp39

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https://www.readbyqxmd.com/read/28447728/high-expression-of-ubiquitin-specific-peptidase-39-is-associated-with-the-development-of-vascular-remodeling
#1
Shuai He, Wei Zhong, Li Yin, Yifei Wang, Zhibing Qiu, Gang Song
Vascular remodeling is the primary cause underlying the failure of angioplasty surgeries, including vascular stenting, transplant vasculopathy and vein grafts. Multiple restenosis‑associated proteins and genes have been identified to account for this. In the present study, the functions of ubiquitin‑specific peptidase 39 (USP39) were investigated in the context of two vascular remodeling models (a mouse common carotid artery ligation and a pig bilateral saphenous vein‑carotid artery interposition graft)...
May 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28413501/usp39-regulates-the-growth-of-smmc-7721-cells-via-foxm1
#2
Xianwen Yuan, Xitai Sun, Xiaolei Shi, Chunping Jiang, Decai Yu, Weiwei Zhang, Yitao Ding
The present study investigated ubiquitin specific peptidase 39 (USP39) gene knockdown on SMMC-7721 cells in vitro and in vivo, and the role of USP39 in regulating the growth of hepatocellular carcinoma (HCC). Two small interfering RNAs (siRNA) were constructed, which targeted the USP39 gene and control sequences were synthesized and inserted into a pGCSIL-GFP lentiviral vector. The full length of USP39 cDNA was amplified by polymerase chain reaction (PCR) and cloned into pEGFP-N2, and the recombinant plasmids were transfected into cells...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28403900/knockdown-of-ubiquitin-specific-peptidase-39-inhibited-the-growth-of-osteosarcoma-cells-and-induced-apoptosis-in-vitro
#3
Zhihua Gan, Kun Han, Shuchen Lin, Haiyan Hu, Zan Shen, Daliu Min
BACKGROUND: Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. METHODS: The mRNA and DNA copies of USP39 were increased in osteosarcoma cancer tissues compared with the one in human normal tissues according to datasets from the publicly available Oncomine database...
April 12, 2017: Biological Research
https://www.readbyqxmd.com/read/28286270/usp39-a-direct-target-of-microrna-133a-promotes-progression-of-pancreatic-cancer-via-the-akt-pathway
#4
Jing Cai, Tiande Liu, Peng Huang, Wei Yan, Changkuo Guo, Le Xiong, Anwen Liu
Ubiquitin specific protease 39 (USP39) is one of the deubiquitinating enzymes without ubiquitin protease activity, which has been implicated in the progression of several cancers. However, the role of USP39 in pancreatic cancer (PC) is largely unknown. In present study, we found that USP39 expression was elevated in PC tissues than adjacent non-tumor tissues. Importantly, we demonstrated that overexpression of USP39 is closely correlated with tumor progression and poor survival in PC patients. Furthermore, high USP39 expression was observed in PC cell lines and ectopic expression of USP39 significantly enhanced in vitro cell proliferation and promoted in vivo tumor growth, whereas silencing USP39 suppressed growth of PC cells...
April 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28259917/usp39-promotes-colorectal-cancer-growth-and-metastasis-through-the-wnt-%C3%AE-catenin-pathway
#5
Xianwen Yuan, Xitai Sun, Xiaolei Shi, Hao Wang, Guoyi Wu, Chunping Jiang, Decai Yu, Weiwei Zhang, Bin Xue, Yitao Ding
In the present study, we first examined the expression of USP39 protein using tissue array containing 90 colorectal cancer (CRC) tissues and 9 clinical samples, and observed that it has significantly higher expression in cancer tissues as compared to the corresponding adjacent normal tissues. Also, we tested USP39 expression level in four CRC cancer cell lines and identified that it indeed had higher expression in all these CRC cell lines. In addition, its knockdown inhibited not only the cell growth of SW480 and HT29 cells, but also the cell migration and invasion...
April 2017: Oncology Reports
https://www.readbyqxmd.com/read/28154181/usp39-deubiquitinase-is-essential-for-kras-oncogene-driven-cancer
#6
Julia M Fraile, Eusebio Manchado, Amaia Lujambio, Víctor Quesada, Diana Campos-Iglesias, Thomas R Webb, Scott W Lowe, Carlos López-Otín, José M P Freije
KRAS is the most frequently mutated oncogene in human cancer, but its therapeutic targeting remains challenging. Here, we report a synthetic lethal screen with a library of deubiquitinases and identify USP39, which encodes an essential splicing factor, as a critical gene for the viability of KRAS-dependent cells. We show that splicing fidelity inhibitors decrease preferentially the proliferation rate of KRAS-active cells. Moreover, depletion of DHX38, encoding an USP39-interacting splicing factor, also reduces the viability of these cells...
March 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27629785/ubiquitin-specific-protease-39-is-overexpressed-in-human-lung-cancer-and-promotes-tumor-cell-proliferation-in-vitro
#7
Zhifeng Lin, Liwen Xiong, Qiang Lin
Lung cancer is the most frequent cancer in the world. Previous studies have shown that ubiquitin-specific protease 39 (USP39) is upregulated in several cancers and associated with tumor malignant characters. However, the effects of USP39 in lung cancer have not been well understood. In the present study, we found USP39 was generally expressed higher in human lung cancer tissues than in normal tissues by Oncomine database mining, qRT-PCR, and western blot assay. Knockdown of USP39 expression markedly reduced the proliferative and colony-forming ability of lung cancer cell lines 95D and A549...
November 2016: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27456357/knockdown-of-usp39-induces-cell-cycle-arrest-and-apoptosis-in-melanoma
#8
Yuan Zhao, Bo Zhang, Yu Lei, Jingying Sun, Yaohua Zhang, Sen Yang, Xuejun Zhang
The spliceosome machinery composed of multimeric protein complexes guides precursor messenger RNAs (mRNAs) (pre-mRNAs) splicing in eukaryotic cells. Spliceosome components have been shown to be downregulated in cancer and could be a promising molecular target for anticancer therapy. The ubiquitin-specific protease 39 (USP39) is essential for pre-mRNA splicing, and upregulated USP39 expression is noted in a variety of cancers. However, the role of USP39 in the development and progression of melanoma remains unclear...
October 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27175747/lentivirus-mediated-inhibition-of-usp39-suppresses-the-growth-of-gastric-cancer-cells-via-parp-activation
#9
Xinbao Wang, Qiming Yu, Ling Huang, Pengfei Yu
Gastric cancer (GC) is the second most common cause of cancer-associated mortality worldwide. Ubiquitin-specific peptidase 39 (USP39) has important roles in mRNA processing and has been reported to be involved in the growth of breast cancer cells. However, the roles of USP39 in GC have remained to be investigated, which was the aim of the present study. A lentivirus expressing short hairpin RNA targeting USP39 was constructed and transfected into MGC80‑3 cells. Suppression of USP39 expression significantly decreased the proliferation and colony forming ability of MGC80‑3 cells as indicated by an MTT and a clonogenic assay, respectively...
July 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/26959883/overexpression-of-usp39-predicts-poor-prognosis-and-promotes-tumorigenesis-of-prostate-cancer-via-promoting-egfr-mrna-maturation-and-transcription-elongation
#10
Yi Huang, Xiu-Wu Pan, Lin Li, Lu Chen, Xi Liu, Jian-Lei Lu, Xiao-Mei Zhu, Hai Huang, Qi-Wei Yang, Jian-Qing Ye, Si-Shun Gan, Lin-Hui Wang, Yi Hong, Dan-Feng Xu, Xin-Gang Cui
Castration resistance is a serious problem facing clinical treatment of prostate cancer (PCa). The underlying molecular mechanisms of acquired proliferation ability of tumor cells upon androgen deprivation are largely undetermined. In the present study, we identified that ubiquitin specific peptidase 39 (USP39) was significantly upregulated in PCa samples and cell lines. Elevated USP39 expression was positively correlated with Gleason score, predicted a poor outcome, and functioned as an independent risk factor for biochemical recurrence (BCR) especially in patients with a Gleason score ≤7...
April 19, 2016: Oncotarget
https://www.readbyqxmd.com/read/26835714/knockdown-of-usp39-by-lentivirus-mediated-rna-interference-suppresses-the-growth-of-oral-squamous-cell-carcinoma
#11
Ke-Yi Li, Jie Zhang, Li-Cheng Jiang, Bin Zhang, Chun-Peng Xia, Kai Xu, Hai-Ying Chen, Qiao-Zhi Yang, Shu-Wei Liu, Hong Zhu
BACKGROUND: Oral squamous cell carcinoma (OSCC) is a frequently diagnosed life-threatening oral cancer worldwide and has become one of the leading causes of cancer-related mortality. However, the pathogenesis of this disease is very limited. OBJECTIVE: In this study, we aimed to investigate the functional relationship between OSCC and a potential tumor related gene ubiquitin-specific proteases 39 (USP39). METHODS: The lentivirus-based RNA interference was utilized to knock down USP39 expression in human OSCC CAL27 cells...
2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/26303214/reduced-usp39-expression-inhibits-malignant-proliferation-of-medullary-thyroid-carcinoma-in-vitro
#12
Yong An, Shuwen Yang, Kai Guo, Ben Ma, Yu Wang
BACKGROUND: Medullary thyroid carcinoma (MTC) constitutes approximately 5 % of all thyroid cancers and carries a worse prognosis than other differentiated thyroid cancers. Targeted therapies are being investigated for systemic treatment of MTC. Ubiquitin-specific peptidase 39 (USP39) functions in pre-mRNA splicing as a component of the U4/U6-U5 tri-snRNP and also participates in spindle checkpoint and cytokinesis. In this study, we aimed to evaluate the potential role in MTC. METHODS: We used lentivirus-delivered short hairpin RNA (shRNA) to silence USP39 expression in one MTC cell line TT...
2015: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/26081192/usp39-promotes-the-growth-of-human-hepatocellular-carcinoma-in-vitro-and-in-vivo
#13
Xianwen Yuan, Xitai Sun, Xiaolei Shi, Chunping Jiang, Decai Yu, Weiwei Zhang, Wenxian Guan, Jianxin Zhou, Yafu Wu, Yudong Qiu, Yitao Ding
Ubiquitin specific protease 39 (USP39) plays an important role in mRNA splicing. In the present study, we investigated the role of USP39 in regulating the growth of hepatocellular carcinoma (HCC). We detected USP39 expression in more than 100 HCC clinical samples. The USP39 expression was significantly higher in the tumor tissues compared to the adjacent normal tissues, and was strongly associated with the pathological grade of HCC. USP39 knockdown inhibited cell proliferation and colony formation in vitro in the HepG2 cells, while upregulation of USP39 promoted tumor cell growth...
August 2015: Oncology Reports
https://www.readbyqxmd.com/read/25889525/lentivirus-mediated-silencing-of-ubiquitin-specific-peptidase-39-inhibits-cell-proliferation-of-human-hepatocellular-carcinoma-cells-in-vitro
#14
Zeya Pan, Hao Pan, Jin Zhang, Yun Yang, Hui Liu, Yuan Yang, Gang Huang, Junsheng Ni, Jian Huang, Weiping Zhou
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcinoma (HCC) cell line SMMC-7721. We knocked down the expression of USP39 through lentivirus mediated RNA interference. The results of qRT-PCR and western blotting assay showed that both the mRNA and protein levels were suppressed efficiently after USP39 specific shRNA was delivered into SMMC-7721 cells...
March 19, 2015: Biological Research
https://www.readbyqxmd.com/read/25812575/lentiviral-vector-mediated-doxycycline-inducible-usp39-shrna-or-cdna-expression-in-triple-negative-breast-cancer-cells
#15
Shihai Liu, Xiangping Liu, Haibo Wang, Quan Zhou, Ye Liang, Aihua Sui, Ruyong Yao, Bin Zhao, Ming Sun
Triple-negative breast cancer (TNBC), characterized by distinct biological and clinicopathological features, has a poor prognosis due to lack of effective therapeutic targets. Our previous data revealed that high levels of USP39 were selectively present in TNBC samples compared with their normal breast tissue samples and USP39 was also expressed at different levels in cultured TNBC cells and normal breast cells. Yet, the underlying cellular and molecular mechanisms of USP39 remain unclear. In the present study, we describe a doxycycline (DOX)-regulated lentiviral vector system expressing shRNA or cDNA of the USP39 gene in the TNBC cell line MDA-MB-231...
May 2015: Oncology Reports
https://www.readbyqxmd.com/read/25027693/important-role-of-sumoylation-of-spliceosome-factors-in-prostate-cancer-cells
#16
Donghua Wen, Zhijian Xu, Li Xia, Xinyi Liu, Yaoyao Tu, Hu Lei, Weiwei Wang, Tongdan Wang, Lili Song, Chunmin Ma, Hanzhang Xu, Weiliang Zhu, Guoqiang Chen, Yingli Wu
Sentrin/SUMO (small ubiquitin-like modifier)-specific proteases (SENPs) have been implicated in the development of prostate cancer. However, due to the low abundance of SUMO-modified proteins and high activity of SENPs, the SUMO substrates affected by SENPs in prostate cancer cells are largely unknown. Here, we identified SI2, a novel cell-permeable SENP-specific inhibitor, by high-throughput screening. Using SI2 as a way of inhibiting the activity of SENPs and the SUMO stably transfected PC3 cells as a prostate cancer model, in combination with the stable isotope labeling with amino acids (SILAC) quantitative proteomic technique, we identified more than 900 putative target proteins of SUMO, in which 231 proteins were further subjected to bioinformatic analysis...
August 1, 2014: Journal of Proteome Research
https://www.readbyqxmd.com/read/24993970/expression-analysis-of-genes-of-ubiquitin-proteasome-protein-degradation-system-in-mptp-induced-mice-models-of-early-stages-of-parkinson-s-disease
#17
E V Filatova, M I Shadrina, A Kh Alieva, A A Kolacheva, P A Slominsky, M V Ugrumov
Parkinson's disease (PD) is the second most common severe neurodegenerative disorder that is characterized by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta (SNpc) region of the brain. However, causes and mechanisms of the development of this disorder are still not fully understood. At the same time, it is well known that dysfunction of the ubiquitin-proteasome protein degradation system (UPPDS) is one of the major mechanisms of the pathogenesis of PD. In this study we have investigated alterations in expression of Uchl3, Ubr7, Ube3c, Usp19, Usp39, Ube2k, Ube2d3, Ube2m, Ube2g1 genes, which are directly involved in the functioning of the UPPDS, using the real-time PCR in mice with the MPTP-induced pre-symptomatic and early symptomatic stages of PD...
May 2014: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/24681967/the-crystal-structure-of-s-cerevisiae-sad1-a-catalytically-inactive-deubiquitinase-that-is-broadly-required-for-pre-mrna-splicing
#18
Haralambos Hadjivassiliou, Oren S Rosenberg, Christine Guthrie
Sad1 is an essential splicing factor initially identified in a genetic screen in Saccharomyces cerevisiae for snRNP assembly defects. Based on sequence homology, Sad1, or USP39 in humans, is predicted to comprise two domains: a zinc finger ubiquitin binding domain (ZnF-UBP) and an inactive ubiquitin-specific protease (iUSP) domain, both of which are well conserved. The role of these domains in splicing and their interaction with ubiquitin are unknown. We first used splicing microarrays to analyze Sad1 function in vivo and found that Sad1 is critical for the splicing of nearly all yeast intron-containing genes...
May 2014: RNA
https://www.readbyqxmd.com/read/24451210/lateral-transfer-of-eukaryotic-ribosomal-rna-genes-an-emerging-concern-for-molecular-ecology-of-microbial-eukaryotes
#19
Akinori Yabuki, Takashi Toyofuku, Kiyotaka Takishita
Ribosomal RNA (rRNA) genes are widely utilized in depicting organismal diversity and distribution in a wide range of environments. Although a few cases of lateral transfer of rRNA genes between closely related prokaryotes have been reported, it remains to be reported from eukaryotes. Here, we report the first case of lateral transfer of eukaryotic rRNA genes. Two distinct sequences of the 18S rRNA gene were detected from a clonal culture of the stramenopile, Ciliophrys infusionum. One was clearly derived from Ciliophrys, but the other gene originated from a perkinsid alveolate...
July 2014: ISME Journal
https://www.readbyqxmd.com/read/24126978/lentivirus-mediated-inhibition-of-usp39-suppresses-the-growth-of-breast-cancer-cells-in-vitro
#20
Haibo Wang, Xiaojun Ji, Xiangping Liu, Ruyong Yao, Jingwei Chi, Shihai Liu, Yu Wang, Weihong Cao, Quan Zhou
Worldwide, breast cancer is the most frequently diagnosed life-threatening cancer in women and is the leading cause of cancer-related mortality among women. It is extremely rare but highly lethal in men. The deubiquitinating enzyme USP39 plays important roles in mRNA processing, and our previous data showed that high levels of USP39 are selectively present in different types of human breast tumor cells. The potential of USP39 as a therapeutic target for breast cancer was investigated. The expression levels of USP39 protein in 23 breast cancer specimens were quantified using an immunohistochemical assay and were found to have high levels in human breast cancer tissues when compared to these levels in normal breast tissues...
December 2013: Oncology Reports
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