Long Liao, Yan He, Shu-Jun Li, Xiao-Mei Yu, Zhi-Chao Liu, Yi-Yao Liang, Han Yang, Jing Yang, Guo-Geng Zhang, Chun-Miao Deng, Xian Wei, Yi-Dong Zhu, Tao-Yang Xu, Can-Can Zheng, Chao Cheng, Ang Li, Zhi-Gang Li, Jin-Bao Liu, Bin Li
Posttranslational modifications add tremendous complexity to proteomes; however, gaps remain in knowledge regarding the function and regulatory mechanism of newly discovered lysine acylation modifications. Here, we compared a panel of non-histone lysine acylation patterns in metastasis models and clinical samples, and focused on 2-hydroxyisobutyrylation (Khib) due to its significant upregulation in cancer metastases. By the integration of systemic Khib proteome profiling in 20 paired primary esophageal tumor and metastatic tumor tissues with CRISPR/Cas9 functional screening, we identified N-acetyltransferase 10 (NAT10) as a substrate for Khib modification...
March 8, 2023: Cell Research