keyword
https://read.qxmd.com/read/37910177/functional-insights-of-three-ring-finger-peroxins-in-the-life-cycle-of-the-insect-pathogenic-fungus-beauveria-bassiana
#1
JOURNAL ARTICLE
Jia-Hui Lei, Ting-Fei Sun, Ming-Guang Feng, Sheng-Hua Ying
Peroxisomes play important roles in fungal physiological processes. The RING-finger complex consists of peroxins Pex2, Pex10, and Pex12 and is essential for recycling of receptors responsible for peroxisomal targeting of matrix proteins. In this study, these three peroxins were functionally characterized in the entomopathogenic fungus Beauveria bassiana (Bb). These three peroxins are associated with peroxisomes, in which BbPex2 interacted with BbPex10 and BbPex12. Ablation of these peroxins did not completely block the peroxisome biogenesis, but abolish peroxisomal targeting of matrix proteins via both PTS1 and PTS2 pathways...
November 1, 2023: Current Genetics
https://read.qxmd.com/read/36616260/differential-antinociceptive-efficacy-of-peel-extracts-and-lyophilized-juices-of-three-varieties-of-mexican-pomegranate-punica-granatum-l-in-the-formalin-test
#2
JOURNAL ARTICLE
José Antonio Guerrero-Solano, Mirandeli Bautista, Josué Vidal Espinosa-Juárez, Luis Alfonso Moreno-Rocha, Gabriel Betanzos-Cabrera, Liana Claudia Salanță, Minarda De la O Arciniega, Elena G Olvera-Hernández, Osmar Antonio Jaramillo-Morales
Pharmacological treatment of pain often causes undesirable effects, so it is necessary to look for natural, safe, and effective alternatives to alleviate painful behavior. In this context, it is known that different parts of pomegranate have been widely consumed and used as preventive and therapeutic agents since ancient times. For example, it has been shown to have an antinociceptive effect, however, there are many varieties. Each part has been found to display unique and attractive pharmacological activities...
December 27, 2022: Plants (Basel, Switzerland)
https://read.qxmd.com/read/36421406/structure-and-function-of-the-peroxisomal-ubiquitin-ligase-complex
#3
JOURNAL ARTICLE
Peiqiang Feng, Michael L Skowyra, Tom A Rapoport
Peroxisomes are membrane-bounded organelles that exist in most eukaryotic cells and are involved in the oxidation of fatty acids and the destruction of reactive oxygen species. Depending on the organism, they house additional metabolic reactions that range from glycolysis in parasitic protozoa to the production of ether lipids in animals and antibiotics in fungi. The importance of peroxisomes for human health is revealed by various disorders - notably the Zellweger spectrum - that are caused by defects in peroxisome biogenesis and are often fatal...
November 24, 2022: Biochemical Society Transactions
https://read.qxmd.com/read/35768507/a-peroxisomal-ubiquitin-ligase-complex-forms-a-retrotranslocation-channel
#4
JOURNAL ARTICLE
Peiqiang Feng, Xudong Wu, Satchal K Erramilli, Joao A Paulo, Pawel Knejski, Steven P Gygi, Anthony A Kossiakoff, Tom A Rapoport
Peroxisomes are ubiquitous organelles that house various metabolic reactions and are essential for human health1-4 . Luminal peroxisomal proteins are imported from the cytosol by mobile receptors, which then recycle back to the cytosol by a poorly understood process1-4 . Recycling requires receptor modification by a membrane-embedded ubiquitin ligase complex comprising three RING finger domain-containing proteins (Pex2, Pex10 and Pex12)5,6 . Here we report a cryo-electron microscopy structure of the ligase complex, which together with biochemical and in vivo experiments reveals its function as a retrotranslocation channel for peroxisomal import receptors...
July 2022: Nature
https://read.qxmd.com/read/34903883/peroxisomal-%C3%AE-oxidation-acts-as-a-sensor-for-intracellular-fatty-acids-and-regulates-lipolysis
#5
JOURNAL ARTICLE
Lianggong Ding, Wenfei Sun, Miroslav Balaz, Anyuan He, Manuel Klug, Stefan Wieland, Robert Caiazzo, Violeta Raverdy, Francois Pattou, Philippe Lefebvre, Irfan J Lodhi, Bart Staels, Markus Heim, Christian Wolfrum
To liberate fatty acids (FAs) from intracellular stores, lipolysis is regulated by the activity of the lipases adipose triglyceride lipase (ATGL), hormone-sensitive lipase and monoacylglycerol lipase. Excessive FA release as a result of uncontrolled lipolysis results in lipotoxicity, which can in turn promote the progression of metabolic disorders. However, whether cells can directly sense FAs to maintain cellular lipid homeostasis is unknown. Here we report a sensing mechanism for cellular FAs based on peroxisomal degradation of FAs and coupled with reactive oxygen species (ROS) production, which in turn regulates FA release by modulating lipolysis...
December 13, 2021: Nature metabolism
https://read.qxmd.com/read/34016526/zebrafish-model-of-human-zellweger-syndrome-reveals-organ-specific-accumulation-of-distinct-fatty-acid-species-and-widespread-gene-expression-changes
#6
JOURNAL ARTICLE
Shigeo Takashima, Shoko Takemoto, Kayoko Toyoshi, Akiko Ohba, Nobuyuki Shimozawa
In Zellweger syndrome (ZS), lack of peroxisome function causes physiological and developmental abnormalities in many organs such as the brain, liver, muscles, and kidneys, but little is known about the exact pathogenic mechanism. By disrupting the zebrafish pex2 gene, we established a disease model for ZS and found that it exhibits pathological features and metabolic changes similar to those observed in human patients. By comprehensive analysis of the fatty acid profile, we found organ-specific accumulation and reduction of distinct fatty acid species, such as an accumulation of ultra-very-long-chain polyunsaturated fatty acids (ultra-VLC-PUFAs) in the brains of pex2 mutant fish...
July 2021: Molecular Genetics and Metabolism
https://read.qxmd.com/read/33565068/-clinical-characterization-and-genetic-analysis-of-a-newborn-with-chromosome-8q21-11-deletion-syndrome
#7
JOURNAL ARTICLE
Suli Li, Weiqing Wu, Jiansheng Xie, Haifei Li
OBJECTIVE: To explore the genetic etiology for a newborn with corneal opacity. METHODS: The neonate and her parents were subjected to routine G-banding chromosomal karyotyping analysis. Copy number variation (CNV) was analyzed with low-coverage whole-genome sequencing (WGS) and single nucleotide polymorphism microarray (SNP array). RESULTS: No karyotypic abnormality was found in the newborn and her parents. Low-coverage WGS has identified a de novo 5...
February 10, 2021: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://read.qxmd.com/read/33509482/studies-on-the-gushing-potential-of-penicillium-expansum
#8
JOURNAL ARTICLE
Lisa M Frisch, Magdalena A Mann, David N Marek, Melanie Baudrexl, Rudi F Vogel, Ludwig Niessen
Gushing describes the spontaneous excessive over-foaming of carbonated beverages leading to considerable economic losses and reputational damages to the beverage industry. Surface-active proteins produced by filamentous fungi are involved in the induction of gushing. In the current study, the role of Penicillium expansum in sparkling wine gushing was investigated. Almost 40 P. expansum strains were analyzed regarding their ability to secrete surface-active proteins and to induce gushing in carbonated water as a model system and in sparkling wine...
January 2021: Food Research International
https://read.qxmd.com/read/33240873/functional-peroxisomes-are-essential-for-efficient-cholesterol-sensing-and-synthesis
#9
JOURNAL ARTICLE
Khanichi N Charles, Janis E Shackelford, Phyllis L Faust, Steven J Fliesler, Herbert Stangl, Werner J Kovacs
Cholesterol biosynthesis is a multi-step process involving several subcellular compartments, including peroxisomes. Cells adjust their sterol content by both transcriptional and post-transcriptional feedback regulation, for which sterol regulatory element-binding proteins (SREBPs) are essential; such homeostasis is dysregulated in peroxisome-deficient Pex2 knockout mice. Here, we compared the regulation of cholesterol biosynthesis in Chinese hamster ovary (CHO-K1) cells and in three isogenic peroxisome-deficient CHO cell lines harboring Pex2 gene mutations...
2020: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/33163488/peroxisome-deficiency-impairs-bdnf-signaling-and-memory
#10
JOURNAL ARTICLE
Yuichi Abe, Yoshiki Nishimura, Kaori Nakamura, Shigehiko Tamura, Masanori Honsho, Hiroshi Udo, Toshihide Yamashita, Yukio Fujiki
Peroxisome is an intracellular organelle that functions in essential metabolic pathways including β-oxidation of very-long-chain fatty acids and biosynthesis of plasmalogens. Peroxisome biogenesis disorders (PBDs) manifest severe dysfunction in multiple organs including central nervous system (CNS), whilst the pathogenic mechanisms are largely unknown. We recently reported that peroxisome-deficient neural cells secrete an increased level of brain-derived neurotrophic factor (BDNF), resulting in the cerebellar malformation...
2020: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/32939436/novel-mutations-and-genes-that-impact-on-growth-in-short-stature-of-undefined-aetiology-the-epigrow-study
#11
JOURNAL ARTICLE
Reena Perchard, Philip George Murray, Antony Payton, Georgina Lee Highton, Andrew Whatmore, Peter Ellis Clayton
Background: Children with short stature of undefined aetiology (SS-UA) may have undiagnosed genetic conditions. Purpose: To identify mutations causing short stature (SS) and genes related to SS, using candidate gene sequence data from the European EPIGROW study. Methods: First, we selected exonic single nucleotide polymorphisms (SNPs), in cases and not controls, with minor allele frequency (MAF) < 2%, whose carriage fitted the mode of inheritance...
October 1, 2020: Journal of the Endocrine Society
https://read.qxmd.com/read/32733884/peroxisome-deficiency-and-hif-2%C3%AE-signaling-are-negative-regulators-of-ketohexokinase-expression
#12
JOURNAL ARTICLE
Tanja Eberhart, Miriam J Schönenberger, Katharina M Walter, Khanichi N Charles, Phyllis L Faust, Werner J Kovacs
Ketohexokinase (KHK) is the first and rate-limiting enzyme of fructose metabolism. Expression of the two alternatively spliced KHK isoforms, KHK-A and KHK-C, is tissue-specific and KHK-C is predominantly expressed in liver, kidney and intestine and responsible for the fructose-catabolizing function. While KHK isoform choice has been linked to the development of disorders such as obesity, diabetes, cardiovascular disease and cancer, little is known about the regulation of total KHK expression. In the present study, we investigated how hypoxic signaling influences fructose metabolism in the liver...
2020: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/32656702/multiple-transcriptomic-analyses-and-characterization-of-pathogen-related-core-effectors-and-lysm-family-members-reveal-their-differential-roles-in-fungal-growth-and-pathogenicity-in-penicillium-expansum
#13
JOURNAL ARTICLE
Danyang Chen, Guangwei Li, Jia Liu, Michael Wisniewski, Samir Droby, Elena Levin, Shengxiong Huang, Yongsheng Liu
Penicillium expansum is a destructive phytopathogen causing postharvest decay on many stored fruits. To develop effective and safe management strategies, it is important to investigate its pathogenicity-related mechanisms. In this study, a bioinformatic pipeline was constructed and 50 core effector genes were identified in P. expansum using multiple RNA-seq data sets and their putative functions were implicated by comparatively homologous analyses using pathogen-host interaction database. To functionally characterize P...
July 12, 2020: Molecular Genetics and Genomics: MGG
https://read.qxmd.com/read/32457250/decreased-deformed-defective-how-hiv-1-vpu-targets-peroxisomes
#14
COMMENT
Kristina Hopfensperger, Daniel Sauter
Peroxisomes are found in essentially all eukaryotic cells and have been described as important hubs in innate sensing and the induction of type III interferons upon viral infection. Nevertheless, it remains poorly investigated how viral pathogens modulate biogenesis or function of peroxisomes to evade innate sensing and restriction. In a recent study, Hobman and colleagues found that the accessory viral protein u (Vpu) of HIV-1 inhibits peroxisome activity by depleting cellular peroxisome pools. This depletion could be ascribed to a Vpu-dependent induction of four microRNAs (miRNAs) that suppress the expression of peroxisomal biogenesis factors PEX2, PEX7, PEX11B, and PEX13...
May 26, 2020: MBio
https://read.qxmd.com/read/31704369/the-roles-of-fgpex2-and-fgpex12-in-virulence-and-lipid-metabolism-in-fusarium-graminearum
#15
JOURNAL ARTICLE
Lina Wang, Li Zhang, Chunjie Liu, Shaohua Sun, Aixin Liu, Yuancun Liang, Jinfeng Yu
Fusarium head blight (FHB) is a wheat disease with a worldwide prevalence, caused by Fusarium graminearum. Peroxisomes are ubiquitous in eukaryotic cells and are involved in various biochemical phenomena. FgPEX2 and FgPEX12 encode RING-finger peroxins PEX2 and PEX12 in F. graminearum. This study aimed to functionally characterize FgPEX2 and FgPEX12 in F. graminearum. We constructed deletion mutants of FgPEX2 and FgPEX12 via homologous recombination. The ΔPEX2 and ΔPEX12 mutants displayed defects in sexual and asexual development, virulence, cell wall integrity (CWI), and lipid metabolism...
November 5, 2019: Fungal Genetics and Biology: FG&B
https://read.qxmd.com/read/31131315/usp30-protector-of-peroxisomes-and-mitochondria
#16
JOURNAL ARTICLE
Victoria Riccio, G Angus McQuibban, Peter Kijun Kim
In our recent publication, we describe a mechanism by which peroxisomes are protected from degradation by autophagy under basal conditions. Taking a page from mitophagy, peroxisomes also recruit the mitochondria deubiquitinating enzyme USP30 to counter the action of PEX2, the peroxisomal E3 ubiquitin ligase to regulate pexophagy.
2019: Molecular & Cellular Oncology
https://read.qxmd.com/read/31046663/genomic-analyses-of-aminoacyl-trna-synthetases-from-human-infecting-helminths
#17
JOURNAL ARTICLE
Preeti Goel, Suhel Parvez, Amit Sharma
BACKGROUND: Helminth infections affect ~ 60% of the human population that lives in tropical and subtropical regions worldwide. These infections result in diseases like schistosomiasis, lymphatic filariasis, river blindness and echinococcosis. Here we provide a comprehensive computational analysis of the aminoacyl tRNA synthetase (aaRS) enzyme family from 27 human-infecting helminths. Our analyses support the idea that several helminth aaRSs can be targeted for drug repurposing or for development of new drugs...
May 2, 2019: BMC Genomics
https://read.qxmd.com/read/30700497/deubiquitinating-enzyme-usp30-maintains-basal-peroxisome-abundance-by-regulating-pexophagy
#18
JOURNAL ARTICLE
Victoria Riccio, Nicholas Demers, Rong Hua, Miluska Vissa, Derrick T Cheng, Amy Wong Strilchuk, Yuqing Wang, G Angus McQuibban, Peter Kijun Kim
The regulation of organelle abundance is critical for cell function and survival; however, the mechanisms responsible are not fully understood. In this study, we characterize a role of the deubiquitinating enzyme USP30 in peroxisome maintenance. Peroxisomes are highly dynamic, changing in abundance in response to metabolic stress. In our recent study identifying the role of USP30 in mitophagy, we observed USP30 to be localized to punctate structures resembling peroxisomes. We report here that USP30, best known as a mitophagy regulator, is also necessary for regulating pexophagy, the selective autophagic degradation of peroxisomes...
March 4, 2019: Journal of Cell Biology
https://read.qxmd.com/read/30366024/isoform-specific-domain-organization-determines-conformation-and-function-of-the-peroxisomal-biogenesis-factor-pex26
#19
JOURNAL ARTICLE
Philipp Guder, Amelie S Lotz-Havla, Mathias Woidy, Dunja D Reiß, Marta K Danecka, Ulrich A Schatz, Marc Becker, Regina Ensenauer, Philipp Pagel, Lars Büttner, Ania C Muntau, Søren W Gersting
Peroxisomal biogenesis factor PEX26 is a membrane anchor for the multi-subunit PEX1-PEX6 protein complex that controls ubiquitination and dislocation of PEX5 cargo receptors for peroxisomal matrix protein import. PEX26 associates with the peroxisomal translocation pore via PEX14 and a splice variant (PEX26Δex5) of unknown function has been reported. Here, we demonstrate PEX26 homooligomerization mediated by two heptad repeat domains adjacent to the transmembrane domain. We show that isoform-specific domain organization determines PEX26 oligomerization and impacts peroxisomal β-oxidation and proliferation...
October 23, 2018: Biochimica et Biophysica Acta. Molecular Cell Research
https://read.qxmd.com/read/29924881/hansenula-polymorpha-aat2p-is-targeted-to-peroxisomes-via-a-novel-pex20p-dependent-pathway
#20
JOURNAL ARTICLE
Ann S Thomas, Arjen M Krikken, Rinse de Boer, Chris Williams
Saccharomyces cerevisiae Aat2p contains a peroxisomal targeting signal type-1 and localizes to peroxisomes in oleate-grown cells, but not in glucose-grown cells. Here, we have investigated Aat2p from the yeast Hansenula polymorpha, which lacks a recognizable peroxisomal targeting signal. Aat2p tagged with GFP at its C terminus displays a dual cytosol-peroxisome localization in ethanol-grown cells. The partial peroxisomal localization of Aat2p persisted in the absence of the classical cycling receptors Pex5p and Pex7p but Aat2p targeting to peroxisomes was reduced in cells deleted for the matrix protein import factors PEX1, PEX2 and PEX13...
July 2018: FEBS Letters
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