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https://www.readbyqxmd.com/read/28645481/subcellular-localization-of-glycolytic-enzymes-and-characterization-of-intermediary-metabolism-of-trypanosoma-rangeli
#1
Rocío Rondón-Mercado, Héctor Acosta, Ana J Cáceres, Wilfredo Quiñones, Juan Luis Concepción
Trypanosoma rangeli is a hemoflagellate protist that infects wild and domestic mammals as well as humans in Central and South America. Although this parasite is not pathogenic for human, it is being studied because it shares with Trypanosoma cruzi, the etiological agent of Chagas' disease, biological characteristics, geographic distribution, vectors and vertebrate hosts. Several metabolic studies have been performed with T. cruzi epimastigotes, however little is known about the metabolism of T. rangeli. In this work we present the subcellular distribution of the T...
June 20, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28644989/transcriptome-analysis-shows-activation-of-the-arginine-deiminase-pathway-in-lactococcus-lactis-as-a-response-to-ethanol-stress
#2
Lorena Díez, Ana Solopova, Rocío Fernández-Pérez, Miriam González, Carmen Tenorio, Oscar P Kuipers, Fernanda Ruiz-Larrea
This paper describes the molecular response of Lactococcus lactis NZ9700 to ethanol. This strain is a well-known nisin producer and a lactic acid bacteria (LAB) model strain. Global transcriptome profiling using DNA microarrays demonstrated a bacterial adaptive response to the presence of 2% ethanol in the culture broth and differential expression of 67 genes. The highest up-regulation was detected for those genes involved in arginine degradation through the arginine deiminase (ADI) pathway (20-40 fold up-regulation)...
May 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28639467/ethanol-induced-symptoms-in-patients-receiving-gemcitabine-diluted-from-a-concentrate-for-solution-for-infusion-containing-ethanol
#3
Raúl Diez-Fernández, Rocío Vázquez-Sánchez, Laura López-Esteban, Santos Enrech-Frances, Ana María Sánchez-Peña, Laura Díaz-Paniagua, Miguel Soria-Tristán, José Miguel Martín Martínez, Teresa Molina-García
Purpose Ethanol as an excipient is used to enhance the solubility of gemcitabine, but, sometimes, the dose of ethanol a patient may be given is much higher than the dose considered to be toxic. We aimed to assess ethanol-related symptoms and signs in patients receiving two formulations of gemcitabine, with and without ethanol. Methods A randomized double blind cross-over study was conducted. All patients being treated with gemcitabine received two consecutive doses of the drug, one diluted from a concentrate for solution for infusion (CSI) containing ethanol and the other from a lyophilized powder, without ethanol, which was used as control group...
January 1, 2017: Journal of Oncology Pharmacy Practice
https://www.readbyqxmd.com/read/28636672/metabolomic-profile-and-nucleoside-composition-of-cordyceps-nidus-sp-nov-cordycipitaceae-a-new-source-of-active-compounds
#4
Juan Chiriví, Giovanna Danies, Rocio Sierra, Nicolas Schauer, Sandra Trenkamp, Silvia Restrepo, Tatiana Sanjuan
Cordyceps sensu lato is a genus of arthropod-pathogenic fungi, which have been used traditionally as medicinal in Asia. Within the genus, Ophiocordyceps sinensis is the most coveted and expensive species in China. Nevertheless, harvesting wild specimens has become a challenge given that natural populations of the fungus are decreasing and because large-scale culture of it has not yet been achieved. The worldwide demand for products derived from cultivable fungal species with medicinal properties has increased recently...
2017: PloS One
https://www.readbyqxmd.com/read/28633946/microplastics-in-gut-contents-of-coastal-freshwater-fish-from-r%C3%A3-o-de-la-plata-estuary
#5
Rocío S Pazos, Tomás Maiztegui, Darío C Colautti, Ariel H Paracampo, Nora Gómez
The presence of microplastics (MPs) in gut contents of coastal freshwater fish of the Rio de la Plata estuary was studied. Samples were taken in six sites where 87 fish belonging to 11 species and four feeding habits were captured. Presence of MPs was verified in the 100% of fish. The fibres represented the 96% of MPs found. The number of MPs in gut contents was significantly higher close to sewage discharge. There was not found relationship between number of MPs and fish length, weight or feeding habit. The spatial differences in mean number of MPs in fish observed in this study, suggest that environmental availability of MPs could be of great importance to explain the differences found among sampling sites analysed...
June 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28633944/gene-expression-analysis-identify-a-metabolic-and-cell-function-alterations-as-a-hallmark-of-obesity-without-metabolic-syndrome-in-peripheral-blood-a-pilot-study
#6
Daniel Antonio de Luis, Raquel Almansa, Rocío Aller, Olatz Izaola, E Romero
BACKGROUND: Understanding molecular basis involved in overweight is an important first step in developing therapeutic pathways against excess in body weight gain. OBJECTIVE: The purpose of our pilot study was to evaluate the gene expression profiles in the peripheral blood of obese patients without other metabolic complications. DESIGN: A sample of 17 obese patients without metabolic syndrome and 15 non obese control subjects was evaluated in a prospective way...
June 10, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28629658/fracture-of-the-femur-in-a-patient-with-polyostotic-paget-s-disease
#7
Sagrario María Santos Seoane, Victor Arenas García, María Gallego Villalobos, Rocío Martínez Gutierrez
No abstract text is available yet for this article.
June 16, 2017: Medicina Clínica
https://www.readbyqxmd.com/read/28628193/modulation-of-epithelial-cell-polarity-by-bacterial-pathogens
#8
REVIEW
Rocio Tapia, Sarah E Kralicek, Gail A Hecht
Epithelial cells constitute a physical barrier that aids in protecting the host from microbial pathogens. Polarized epithelial cells contain distinct apical and basolateral membrane domains separated by intercellular junctions, including tight junctions (TJs), which contribute to the maintenance of apical-basal polarity. Polarity complexes also contribute to the establishment of TJ formation. Several pathogens perturb epithelial TJ barrier function and structure in addition to causing a loss of apical-basal polarity...
June 19, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/28627212/efficacy-of-enteral-nutritional-support-after-hospital-discharge-in-major-gastrointestinal-surgery-patients-a-systematic-review
#9
Alfonso Vidal Casariego, Alicia Calleja Fernández, Rocío Villar Taibo, Ana Urioste Fondo, Begoña Pintor de la Maza, Ana Hernández Moreno, Isidoro Cano Rodríguez, María D Ballesteros Pomar
INTRODUCTION: Nutritional support for malnourished patients undergoing gastrointestinal surgery reduces the complication rate and shortens the length of stay. The efficacy of nutritional support after hospital discharge was analyzed in this systematic review. METHODS: The search strategy (nutrition OR "enteral nutrition" OR "nutritional supplements" OR "oral nutritional supplements" OR "sip feed" OR "sip feeding" OR "dietary counseling") AND ("patient discharge" OR discharge OR postdischarge) AND (surgery OR operation OR "surgical procedure") was followed in Medline, CENTRAL, and Trip databases...
June 5, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28627209/anorexia-y-bulimia-nerviosas-difusi%C3%A3-n-virtual-de-la-enfermedad-como-estilo-de-vida
#10
Gina Lladó, Rocío González-Soltero, María José Blanco Fernández de Valderrama
Introducción: la adolescencia es un periodo vulnerable para padecer trastornos de la conducta alimentaria (TCA) como la anorexia y la bulimia nerviosas. La insatisfacción corporal, uno de los factores precipitantes de los TCA, conduce a las adolescentes a la búsqueda de información sobre dietas en internet. En este contexto, las páginas pro-Ana (proanorexia) y pro-Mía (probulimia) difunden contenidos altamente perjudiciales para la salud relacionados con la pérdida de peso y los TCA.Objetivos: en el presente trabajo se analizan la cantidad, el posicionamiento, la calidad y la difusión de las páginas pro-Ana y pro-Mía...
June 5, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28626616/discrimination-of-picea-chihuahuana-martinez-populations-on-the-basis-of-climatic-edaphic-dendrometric-genetic-and-population-traits
#11
Iliana Karina Dominguez-Guerrero, Samantha Del Rocío Mariscal-Lucero, José Ciro Hernández-Díaz, Berthold Heinze, José Ángel Prieto-Ruiz, Christian Wehenkel
BACKGROUND: Picea chihuahuana, which is endemic to Mexico, is currently listed as "Endangered" on the Red List. Chihuahua spruce is only found in the Sierra Madre Occidental (SMO), Mexico. About 42,600 individuals are distributed in forty populations. These populations are fragmented and can be classified into three geographically distinct clusters in the SMO. The total area covered by P. chihuahuana populations is less than 300 ha. A recent study suggested assisted migration as an alternative to the ex situ conservation of P...
2017: PeerJ
https://www.readbyqxmd.com/read/28626084/optimization-of-ras-braf-mutational-analysis-confirms-improvement-in-patient-selection-for-clinical-benefit-to-anti-egfr-treatment-in-metastatic-colorectal-cancer
#12
Cristina Santos, Daniel Azuara, Rocio Garcia-Carbonero, Pilar García Alfonso, Alfredo Carrato, Elena Elez, Auxiliadora Gómez, Ferran Losa, Clara Montagut, Bartomeu Massuti, Valenti Navarro, Mar Varela, Adriana López-Doriga, Victor Moreno, Manuel Valladares, Jose Luis Manzano, José Maria Viéitez, Enrique Aranda, Xavier Sanjuan, Josep Tabernero, Gabriel Capella, Ramon Salazar
In metastatic colorectal cancer (mCRC) recent studies have shown the importance to accurately quantify low-abundance mutations of RAS pathway because anti-EGFR therapy may depend on certain mutation thresholds. We aimed to evaluate the added predictive value of extended RAS panel testing using two commercial assays and a highly sensitive and quantitative digital PCR (dPCR). Tumor samples from 583 mCRC patients treated with anti-EGFR (n=255) or bevacizumab (n=328) based therapies from several clinical trials and retrospective series from the TTD/RTICC Spanish network were analyzed by cobas®, therascreen® and dPCR...
June 16, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28624651/efficacy-and-optimal-duration-of-metallic-stent-in-the-management-of-refractory-anastomotic-stricture-after-liver-transplantation
#13
Sunguk Jang, Mansour A Parsi, Rocio Lopez, Amit Bhatt, John J Vargo
BACKGROUND: Anastomotic bile duct stricture (ABS) remains as one of the most common complications in liver transplant patients. Current practice of endoscopic retrograde cholangiopancreatography (ERCP) with insertion of plastic stent (PS) often requires multiple procedures before achieving stricture resolution. To date, studies utilizing covered self-expandable metallic stent (cSEMS) in ABS management reported varying degree of efficacy. The aim of this study was to analyze long term efficacy of cSEMS in patients with ABS, and identifying factor(s) influencing the probability of stricture resolution...
June 14, 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28623742/effect-of-intensive-ldl-cholesterol-lowering-with-pcsk9-monoclonal-antibodies-on-tendon-xanthoma-regression-in-familial-hypercholesterolemia
#14
Ana M Bea, Sofia Perez-Calahorra, Victoria Marco-Benedi, Itziar Lamiquiz-Moneo, Estibaliz Jarauta, Rocio Mateo-Gallego, Fernando Civeira
BACKGROUND AND AIMS: The effect of LDLc lowering with PCSK9 antibodies on tendon xanthomas (TX) is unknown. METHODS: TX was measured in 24 heterozygous familial hypercholesterolemia (HeFH) cases and in 24 HeFH controls with or without PCSK9 inhibitors for at least one year. RESULTS: Exposure to PCSK9 inhibitors in cases was 2.96 ± 1.33 years. LDLc decreased 80.8 ± 7.66% in cases and 56.9 ± 11.1% in controls. There was a decrease in maximum (-5...
June 8, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28623579/yeast-one-and-two-hybrid-high-throughput-screenings-using-arrayed-libraries
#15
Rocío Sánchez-Montesino, Luis Oñate-Sánchez
Since their original description more than 25 years ago, the yeast one- and two-hybrid systems (Y1H/Y2H) have been used by many laboratories to detect DNA-protein (Y1H) and protein-protein interactions (Y2H). These systems use yeast cells (Saccharomyces cerevisiae) as a eukaryotic "test tube" and are amenable for most labs in the world. The development of highly efficient cloning methods has fostered the generation of large collections of open reading frames (ORFs) for several organisms that have been used for yeast screenings...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28622282/towards-a-neurobiological-understanding-of-alexithymia
#16
REVIEW
Nicolás Meza-Concha, Marcelo Arancibia, Felicia Salas, Rosa Behar, Germán Salas, Hernán Silva, Rocío Escobar
Although the specialized literature on the etiology of alexithymia is controversial, neurobiological research has shown relevant advances. The aim of this review is to analyze the available evidence regarding the neurophysiological bases of alexithymia. A comprehensive review of available articles from Medline/PubMed, EBSCO and SciELO was conducted. Previously, alexithymia was linked to a reduced interhemispheric brain connection. From a childhood traumatic perspective, the right prefrontal cortex and the default mode network would experience alterations, first hypermetabolic (dopaminergic and glutamatergic dysregulation) and then hypometabolic-dissociative (serotonergic and opioid dysregulation), resulting in a distorted interoceptive and emotional awareness...
May 29, 2017: Medwave
https://www.readbyqxmd.com/read/28618099/epec-effector-espf-promotes-crumbs3-endocytosis-and-disrupts-epithelial-cell-polarity
#17
Rocio Tapia, Sarah E Kralicek, Gail A Hecht
Enteropathogenic Escherichia coli (EPEC) uses a type three secretion system to inject effector proteins into host intestinal epithelial cells causing diarrhea. EPEC infection redistributes basolateral proteins β1-integrin and Na(+) /K(+) ATPase to the apical membrane of host cells. The Crumbs (Crb) polarity complex (Crb3/Pals1/Patj) is essential for epithelial cell polarization and tight junction (TJ) assembly. Here we demonstrate that EPEC displaces Crb3 and Pals1 from the apical membrane to the cytoplasm of cultured intestinal epithelial cells and colonocytes of infected mice...
June 15, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28617960/low-prevalence-of-igg-antibodies-against-antigens-of-hev-genotypes-1-and-3-in-women-with-a-high-risk-pregnancy
#18
Baptista-González Héctor, Trueba-Gómez Rocío, Rosenfeld-Mann Fanny, Roque-Álvarez Elsa, Méndez-Sánchez Nahúm
INTRODUCTION: The aim of the study was to assess whether high-risk pregnant women have a higher prevalence of HEV during the perinatal period. MATERIAL AND METHODS: This was a cross-sectional study of 428 patients: Group 1, 127 women with a high-risk pregnancy; Group 2, 97 asymptomatic people with reactivity to HCV or HBV; Group 3, 94 patients with clinical symptoms suggestive of HEV infection; and Group 4, 110 healthy blood donors from an urban area of Mexico City...
June 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28617812/inferring-repeat-protein-energetics-from-evolutionary-information
#19
Rocío Espada, R Gonzalo Parra, Thierry Mora, Aleksandra M Walczak, Diego U Ferreiro
Natural protein sequences contain a record of their history. A common constraint in a given protein family is the ability to fold to specific structures, and it has been shown possible to infer the main native ensemble by analyzing covariations in extant sequences. Still, many natural proteins that fold into the same structural topology show different stabilization energies, and these are often related to their physiological behavior. We propose a description for the energetic variation given by sequence modifications in repeat proteins, systems for which the overall problem is simplified by their inherent symmetry...
June 15, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28617720/development-of-a-trigger-tool-to-identify-adverse-drug-events-in-elderly-patients-with-multimorbidity
#20
María Dolores Toscano Guzmán, Mercedes Galván Banqueri, María José Otero, Eva Rocío Alfaro Lara, Pilar Casajus Lagranja, Bernardo Santos Ramos
PURPOSE: Elderly patients with multimorbidity are especially vulnerable to adverse drug events (ADEs) and had high prevalence rates. Identifying ADEs is essential for enabling timely interventions that can mitigate the adverse events detected and for developing targeted strategies to prevent their occurrence as well as to monitor implementation. The aim of this study was to develop a set with appropriate triggers for detecting potential ADEs in elderly patients with multimorbidity. METHODS: A modified Delphi methodology was used to reach consensus...
June 14, 2017: Journal of Patient Safety
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