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https://www.readbyqxmd.com/read/28287429/d-amino-acid-position-influences-the-anticancer-activity-of-galaxamide-analogs-an-apoptotic-mechanism-study
#1
Defa Bai, Siming Yu, Shenghui Zhong, Bingxin Zhao, Shaoling Qiu, Jianwei Chen, Jignesh Lunagariya, Xiaojian Liao, Shihai Xu
Galaxamide, an extract from Galaxaura filamentosa, is a cyclic pentapeptide containing five l-leucines. Due to the particular cyclic structure and the excellent anticancer activity, synthesis of Galaxamide and its analogs and their subsequent bio-applications have attracted great attention. In the present work, we synthesized six Galaxamide analogs by replacing one of the l-leucines with phenylalanine and varying the d-amino acid position. The anticancer effect of the synthesized Galaxamide analogs was tested against four in vitro human cancer cell lines, human hepatocellular cells (HepG₂), human breast cancer cell (MCF-7), human breast adenocarcinoma cells (MDA-MB-435) and a human cervical carcinoma cell line (Hela)...
March 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28276486/a-potential-regulatory-network-underlying-distinct-fate-commitment-of-myogenic-and-adipogenic-cells-in-skeletal-muscle
#2
Wenjuan Sun, Ting He, Chunfu Qin, Kai Qiu, Xin Zhang, Yanhong Luo, Defa Li, Jingdong Yin
Mechanism controlling myo-adipogenic balance in skeletal muscle is of great significance for human skeletal muscle dysfunction and myopathies as well as livestock meat quality. In the present study, two cell subpopulations with particular potency of adipogenic or myogenic differentiation were isolated from neonatal porcine longissimus dorsi using the preplate method to detect mechanisms underlying distinct fate commitment of myogenic and adipogenic cells in skeletal muscle. Both cells share a common surface expression profile of CD29(+)CD31(-)CD34(-)CD90(+)CD105(+), verifying their mesenchymal origin...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28260165/l-arginine-promotes-protein-synthesis-and-cell-growth-in-brown-adipocyte-precursor-cells-via-the-mtor-signal-pathway
#3
Xi Ma, Meng Han, Defa Li, Shengdi Hu, Kyler R Gilbreath, Fuller W Bazer, Guoyao Wu
L-Arginine has been reported to enhance brown adipose tissue developments in fetal lambs of obese ewes, but the underlying mechanism is unknown. The present study tested the hypothesis that L-arginine stimulates growth and development of brown adipocyte precursor cells (BAPCs) through activation of mammalian target of rapamycin cell signaling. BAPCs isolated from fetal lambs at day 90 of gestation were incubated   for 6 h in arginine-free DMEM, and then cultured in DMEM with concentrations of 50, 100, 200, 500 or 1000 μmol L-arginine/L for 24-96 h...
March 4, 2017: Amino Acids
https://www.readbyqxmd.com/read/28241738/response-of-micrornas-to-cold-treatment-in-the-young-spikes-of-common-wheat
#4
Guoqi Song, Rongzhi Zhang, Shujuan Zhang, Yulian Li, Jie Gao, Xiaodong Han, Mingli Chen, Jiao Wang, Wei Li, Genying Li
BACKGROUND: MicroRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in biotic and abiotic stresses by regulating their target genes. For common wheat, spring frost damage frequently occurs, especially when low temperature coincides with plants at early floral organ differentiation, which may result in significant yield loss. Up to date, the role of miRNAs in wheat response to frost stress is not well understood. RESULTS: We report here the sequencing of small RNA transcriptomes from the young spikes that were treated with cold stress and the comparative analysis with those of the control...
February 28, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28219317/dietary-fiber-gap-and-host-gut-microbiota
#5
Meng Han, Ping Liu, Defa Li, Yuan Li, Xi Ma
Accumulating evidence are dramatically increasing access to the facts that the gut microbiota plays a pivotal role in host metabolism and health, which revealed the possibility of a plethora of associations between gut bacteria and human diseases. Several functional roles carried out by a major class of host's diet, such as fiber. Fiber is the main source of microbiota-accessible carbohydrate in the diet of humans. In modern diet, it is difficult to intake dietary fiber as enough as recommended standard. The low-fiber diet in the modern life, known as fiber gap, can trigger to a substantial depletion of the human gut microbiota diversity and beneficial metabolites...
February 20, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28111434/effects-of-proteinate-complex-zinc-on-growth-performance-hepatic-and-splenic-trace-elements-concentrations-antioxidative-function-and-immune-functions-in-weaned-piglets
#6
Yue She, Qiang Huang, Defa Li, Xiang-Shu Piao
Objective: To assess the effects of proteinate complex zinc (PC-Zn) on growth performance, antioxidative function, trace element concentrations and immune function in weaned piglets. Methods: Three hundred newly weaned barrows (Duroc × Landrace × Yorkshire), 28 days of age, were randomly allotted to 3 dietary groups of 5 replicate pens per group for 4 weeks of feeding. Experimental diets were: (1) zinc deficient diet (ZnD, 24 mg/kg Zn supplementation from ZnSO4), (2) inorganic Zn diet supplemented with 120 mg/kg of Zn from Zn sulfate (ZnSO4), and (3) organic Zn diet supplemented with 120 mg/kg of Zn from PC-Zn...
January 13, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28059666/mtorc1-mediated-nrbf2-phosphorylation-functions-as-a-switch-for-the-class-iii-ptdins3k-and-autophagy
#7
Xi Ma, Shen Zhang, Long He, Yueguang Rong, Livia Wilz Brier, Qiming Sun, Rong Liu, Weiliang Fan, She Chen, Zhenyu Yue, Joungmok Kim, Kun-Liang Guan, Defa Li, Qing Zhong
NRBF2/Atg38 has been identified as the fifth subunit of the macroautophagic/autophagic class III phosphatidylinositol 3-kinase (PtdIns3K) complex, along with ATG14/Barkor, BECN1/Vps30, PIK3R4/p150/Vps15 and PIK3C3/Vps34. However, its functional mechanism and regulation are not fully understood. Here, we report that NRBF2 is a fine tuning regulator of PtdIns3K controlled by phosphorylation. Human NRBF2 is phosphorylated by MTORC1 at S113 and S120. Upon nutrient starvation or MTORC1 inhibition, NRBF2 phosphorylation is diminished...
March 4, 2017: Autophagy
https://www.readbyqxmd.com/read/27930314/direct-evidence-of-interaction-induced-dirac-cones-in-a-monolayer-silicene-ag-111-system
#8
Ya Feng, Defa Liu, Baojie Feng, Xu Liu, Lin Zhao, Zhuojin Xie, Yan Liu, Aiji Liang, Cheng Hu, Yong Hu, Shaolong He, Guodong Liu, Jun Zhang, Chuangtian Chen, Zuyan Xu, Lan Chen, Kehui Wu, Yu-Tzu Liu, Hsin Lin, Zhi-Quan Huang, Chia-Hsiu Hsu, Feng-Chuan Chuang, Arun Bansil, X J Zhou
Silicene, analogous to graphene, is a one-atom-thick 2D crystal of silicon, which is expected to share many of the remarkable properties of graphene. The buckled honeycomb structure of silicene, along with enhanced spin-orbit coupling, endows silicene with considerable advantages over graphene in that the spin-split states in silicene are tunable with external fields. Although the low-energy Dirac cone states lie at the heart of all novel quantum phenomena in a pristine sheet of silicene, a hotly debated question is whether these key states can survive when silicene is grown or supported on a substrate...
December 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27802033/an-azobenzenyl-anion-radical-complex-of-magnesium-synthesis-structure-and-reactivity-studies
#9
Wenshan Ren, Defa Gu
Azobenzenyl anion radical complex of magnesium [HC(C(Me)N-2,6-(i)Pr2C6H3)2]Mg(PhNNPh) (THF) (2) was obtained for the first time through the reaction of [HC(C(Me)N-2,6-(i)Pr2C6H3)2]MgBr (1) with potassium graphite in the presence of azobenzene. Complex 2 is a useful electron-transfer reagent as shown by the reactivity with diphenyl disulfide, diphenyl diselenide, oxygen, sulfur, and Me3SiN3 yielding the magnesium thiolate [HC(C(Me)N-2,6-(i)Pr2C6H3)2]Mg(SPh)(THF) (3), magnesium selenolate [HC(C(Me)N-2,6-(i)Pr2C6H3)2]Mg(SePh)(THF) (4), peroxido complex {[HC(C(Me)N-2,6-(i)Pr2C6H3)2]Mg(THF)}2(μ-η(2)-η(2)-O2) (5), persulfido complex {[HC(C(Me)N-2,6-(i)Pr2C6H3)2]Mg(THF)}2(μ-η(2)-η(2)-S2) (6), and azido complex [HC(C(Me)N-2,6-(i)Pr2C6H3)2]MgN3 (7), respectively...
November 21, 2016: Inorganic Chemistry
https://www.readbyqxmd.com/read/27752660/co-tolerance-of-pt-feox-catalyst-in-both-thermal-catalytic-h2-oxidation-and-electrochemical-co-oxidation-the-effect-of-pt-deficit-electron-state
#10
Lequan Liu, Feng Zhou, Rajesh Kodiyath, Shigenori Ueda, Hideki Abe, Defa Wang, Youquan Deng, Jinhua Ye
CO poisoning of Pt catalysts is one of the major challenges to the commercialization of proton exchange membrane fuel cells. One promising solution is to develop CO-tolerant Pt-based catalysts. A facilely synthesized Pt/FeOx catalyst exhibited outstanding CO tolerance in the oxidation of H2 and electrochemical CO stripping. Light-off temperature of H2O formation over Pt/FeOx was achieved even below 30 °C in the presence of 3000 ppm CO at a space velocity of 18 000 mL g(-1)cat h(-1). For the electrochemical oxidation of CO, the onset and peak potentials decreased by 0...
October 18, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27660021/evaluation-of-available-energy-and-total-tract-digestibility-of-acid-hydrolyzed-ether-extract-in-cottonseed-oil-for-growing-pigs
#11
Jinbiao Zhao, Zhongchao Li, Mingbin Lyu, Ling Liu, Xiangshu Piao, Defa Li
The objective of this study was to determine the effect of inclusion level on the digestible energy (DE), metabolizable energy (ME) and total tract digestibility of acid-hydrolyzed ether extract (AEE) for cottonseed oil when fed to growing pigs. Forty-two barrows (initial BW = 35.51 ± 2.01 kg) were randomly allotted to a completely randomized design with a corn-soybean meal basal diet, five levels of cottonseed oil (2%, 4%, 6%, 8% and 10%) and a 10% soybean oil diet. Each diet was replicated six times with one pig per replicate...
September 19, 2016: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/27611633/effects-of-variety-and-storage-duration-on-the-nutrient-digestibility-and-the-digestible-and-metabolisable-energy-content-of-maize-fed-to-growing-pigs
#12
Lei Zhang, Ling Liu, Defa Li, Quanfeng Li, Xiangshu Piao, Philip A Thacker, Michael A Brown, Changhua Lai
The objective of this research was to determine the effects of variety and storage duration on the nutrient digestibility and the digestible (DE) and metabolisable (ME) energy content in maize when fed to growing pigs. Four maize varieties (LS1, LS2, LS3 and LS4) were hand-harvested from the same growing area in China in early October of 2012. The samples were sun dried to about 14% moisture content and then stored in the warehouse of the Fengning Pig Experiment Base at China Agricultural University for 0, 3 or 10 months...
February 2017: Archives of Animal Nutrition
https://www.readbyqxmd.com/read/27598177/design-and-synthesis-of-analogues-of-marine-natural-product-galaxamide-an-n-methylated-cyclic-pentapeptide-as-potential-anti-tumor-agent-in-vitro
#13
Jignesh Lunagariya, Shenghui Zhong, Jianwei Chen, Defa Bai, Poonam Bhadja, Weili Long, Xiaojian Liao, Xiaoli Tang, Shihai Xu
Herein, we report design and synthesis of novel 26 galaxamide analogues with N-methylated cyclo-pentapeptide, and their in vitro anti-tumor activity towards the panel of human tumor cell line, such as, A549, A549/DPP, HepG2 and SMMC-7721 using MTT assay. We have also investigated the effect of galaxamide and its representative analogues on growth, cell-cycle phases, and induction of apoptosis in SMMC-7721 cells in vitro. Reckon with the significance of conformational space and N-Me aminoacid (aa) comprising this compound template, we designed the analogues with modification in N-Me-aa position, change in aa configuration from l to d aa and substitute one Leu-aa to d/l Phe-aa residue with respective to the parent structure...
September 3, 2016: Marine Drugs
https://www.readbyqxmd.com/read/27563435/erratum-to-use-of-near-infrared-reflectance-spectroscopy-for-the-rapid-determination-of-the-digestible-energy-and-metabolizable-energy-content-of-corn-fed-to-growing-pigs
#14
Juntao Li, Quanfeng Li, Defa Li, Yiqiang Chen, Xiaoxiao Wang, Wenjun Yang, Liying Zhang
[This corrects the article DOI: 10.1186/s40104-016-0105-9.].
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27563166/elevated-plasma-%C3%AE-defensins-hnp1-3-levels-correlated-with-iga1-glycosylation-and-susceptibility-to-iga-nephropathy
#15
COMPARATIVE STUDY
Yuan-Yuan Qi, Xu-Jie Zhou, Fa-Juan Cheng, Hong Zhang
Aim. IgA nephropathy (IgAN) is the most common form of glomerulonephritis. Recent genome-wide association study (GWAS) suggested that DEFA locus (which encodes α-defensins) may play a key role in IgAN. Methods. The levels of α-defensins in 169 IgAN patients and 83 healthy controls were tested by ELISA. Results. We observed that α-defensins human neutrophil peptides 1-3 (HNP1-3) in IgAN patients were elevated compared with healthy controls. The mean levels of α-defensins of 83 healthy controls and 169 IgAN patients were 50 ng/mL and 78...
2016: Disease Markers
https://www.readbyqxmd.com/read/27514477/acetylcholine-producing-t-cells-in-the-intestine-affect-antimicrobial-peptide-expression-and-microbial-diversity
#16
Shobhit Dhawan, Giada De Palma, Rose A Willemze, Francisca Hilbers, Caroline Verseijden, Misha Luyer, Sabine Nuding, Jan Wehkamp, Yuri Souwer, Esther C de Jong, Jurgen Seppen, Rene Mjgj van den Wijngaard, Sven Wehner, Elena F Verdu, Premysl Bercik, Wouter J de Jonge
The cholinergic anti-inflammatory pathway reduces systemic TNF via acetylcholine producing memory T-cells in the spleen. These choline acetyltransferase (ChAT) expressing T-cells are also found in the intestine, where their function is unclear. We aimed to characterize these cells in mouse and human intestine and delineate their function. We made use of the ChAT-eGFP reporter mice. CD4Cre mice were crossed to ChATfl/fl mice to achieve specific deletion of ChAT in CD4+ T-cells. We observed that the majority of ChAT expressing T-cells in the human and mouse intestine have characteristics of Th17 cells, and co-express IL17A, IL22 and RORC...
August 11, 2016: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://www.readbyqxmd.com/read/27505316/influence-of-pachymetry-and-intraocular-pressure-on-dynamic-corneal-response-parameters-in-healthy-patients
#17
Riccardo Vinciguerra, Ahmed Elsheikh, Cynthia J Roberts, Renato Ambrósio, David Sung Yong Kang, Bernardo T Lopes, Emanuela Morenghi, Claudio Azzolini, Paolo Vinciguerra
PURPOSE: To evaluate the influence of pachymetry, age, and intraocular pressure in normal patients and to provide normative values for all dynamic corneal response parameters (DCRs) provided by dynamic Scheimpflug analysis. METHODS: Seven hundred five healthy patients were included in this multicenter retrospective study. The biomechanical response data were analyzed to obtain normative values with their dependence on corrected and clinically validated intraocular pressure estimates developed using the finite element method (bIOP), central corneal thickness (CCT), and age, and to evaluate the influence of bIOP, CCT, and age...
August 1, 2016: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/27493726/use-of-near-infrared-reflectance-spectroscopy-for-the-rapid-determination-of-the-digestible-energy-and-metabolizable-energy-content-of-corn-fed-to-growing-pigs
#18
Juntao Li, Quanfeng Li, Defa Li, Yiqiang Chen, Xiaoxiao Wang, Wunjun Yang, Liying Zhang
BACKGROUND: The ability of near-infrared reflectance spectroscopy (NIRS) to determine the digestible energy (DE) and metabolizable energy (ME) content of corn fed to growing pigs was tested. One hundred and seventeen corn samples, comprising different planting regions and varieties were collected from all over China in a three-year period. The samples were randomly split into a calibration set (n = 88) and a validation set (n = 29). The actual and calculated DE and ME content of the corn samples was determined by digestion-metabolism experiments and the prediction equations of Noblet and Perez (J Anim Sci...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27469890/aspergillus-fumigatus-spore-proteomics-and-genetics-reveal-that-vea-represses-defa-mediated-dna-damage-response
#19
Kwang-Soo Shin, Hee-Soo Park, Young Kim, In-Beom Heo, Young Hwan Kim, Jae-Hyuk Yu
Aspergillus fumigatus reproduces and infects host by forming a high number of small asexual spores (conidia). The velvet proteins are global transcriptional regulators governing the complex process of conidiogenesis in this fungus. Here, to further understand the velvet-mediated regulation, we carried out comparative proteomic analyses of conidia of wild type (WT) and three velvet mutants (ΔveA, ΔvelB and ΔvosA). Cluster analysis of 184 protein spots showing at least 1.5-fold differential accumulation between WT and mutants reveal the clustering of WT- ΔveA and ΔvelB-ΔvosA...
October 4, 2016: Journal of Proteomics
https://www.readbyqxmd.com/read/27464458/effects-of-isoleucine-on-glucose-uptake-through-the-enhancement-of-muscular-membrane-concentrations-of-glut1-and-glut4-and-intestinal-membrane-concentrations-of-na-glucose-co-transporter-1-sglt-1-and-glut2
#20
Shihai Zhang, Qing Yang, Man Ren, Shiyan Qiao, Pingli He, Defa Li, Xiangfang Zeng
Knowledge of regulation of glucose transport contributes to our understanding of whole-body glucose homoeostasis and human metabolic diseases. Isoleucine has been reported to participate in regulation of glucose levels in many studies; therefore, this study was designed to examine the effect of isoleucine on intestinal and muscular GLUT expressions. In an animal experiment, muscular GLUT and intestinal GLUT were determined in weaning pigs fed control or isoleucine-supplemented diets. Supplementation of isoleucine in the diet significantly increased piglet average daily gain, enhanced GLUT1 expression in red muscle and GLUT4 expression in red muscle, white muscle and intermediate muscle (P<0·05)...
August 2016: British Journal of Nutrition
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