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Markus V Heppt, Cecilia Dietrich, Saskia A Graf, Thomas Ruzicka, Julia K Tietze, Carola Berking
Melanoma is a common type of skin cancer with a high propensity to metastasize. Tyrosine kinase inhibitors targeting the mitogen-activated protein kinase (MAPK) pathway and immune checkpoint blockade have recently revolutionized the management of unresectable and metastatic disease. However, acquired resistance and primary non-response to therapy require novel treatment strategies and combinations. The purpose of this review is to provide a brief and up-to-date overview on the clinical management and current trial landscape in melanoma...
2016: Oncology Research and Treatment
Nicolas Majunke, Norman Mangner, Axel Linke, Enno Boudriot, Sandra Erbs, Franziska Tietz, Sabrina Wolff, Stephan Schürer, Gerhard Schuler, Marcus Sandri
OBJECTIVE: Surgical femoral cutdown for decannulation after veno-arterial extracorporeal membrane oxygenation (VA-ECMO) is considered standard practice. However, access-site complications with this technique are not rare. The objective of this study is to evaluate feasibility, safety, and efficacy of a complete percutaneous decannulation procedure after VA-ECMO compared with the conventional surgical cutdown approach. METHODS: In 35 patients who were successfully weaned from VA-ECMO support, femoral artery and vein access sites were closed using a completely percutaneous approach in 15 patients, whereas 20 patients had conventional surgical cutdown for access-site closure...
October 2016: Journal of Invasive Cardiology
Zhengan Zhang, Graham A Hudson, Nilkamal Mahanta, Jonathan I Tietz, Wilfred A van der Donk, Douglas A Mitchell
The biosynthesis of the thiopeptide thiomuracin is a well-orchestrated process involving a multitude of posttranslational modifications. We show that six Cys residues of a precursor peptide are first cyclodehydrated and oxidized to thiazoles in an ordered, but non-linear fashion that is leader peptide-dependent. Then four alcohols are glutamylated and converted to alkenes in a C-to-N terminal directional process that is leader peptide-independent. Finally, two of these alkenes undergo a formal [4+2] cycloaddition to form a tri-thiazole-substituted pyridine macrocycle...
October 4, 2016: Journal of the American Chemical Society
Geoffry A Davis, Atsuko Kanazawa, Mark Aurel Schöttler, Kaori Kohzuma, John E Froehlich, A William Rutherford, Mio Satoh-Cruz, Deepika Minhas, Stefanie Tietz, Amit Dhingra, David M Kramer
The thylakoid proton motive force (pmf) generated during photosynthesis is the essential driving force for ATP production; it is also a central regulator of light capture and electron transfer. We investigated the effects of elevated pmf on photosynthesis in a library of Arabidopsis thaliana mutants with altered rates of thylakoid lumen proton efflux, leading to a range of steady-state pmf extents. We observed the expected pmf-dependent alterations in photosynthetic regulation, but also strong effects on the rate of photosystem II (PSII) photodamage...
October 4, 2016: ELife
Rosiléia Marinho de Quadros, Paulo Henrique Exterchoter Weiss, Sandra Marcia Tietz Marques, Luiz Claudio Miletti
Giardia duodenalis is an enteric parasite that has distinct genetic groups. Human infections are mainly caused by assemblages A and B, although sporadic infections by assemblages C and D have also been reported. Animals can be infected by a wide range of assemblages (A to H). The aim of this study is to identify the assemblages and sub-assemblages of G. duodenalis with zoonotic features in fecal samples of school-aged children, and in dogs that coexist in the same households in Lages, Santa Catarina, Brazil...
2016: Revista do Instituto de Medicina Tropical de São Paulo
Vianney Cortés-González, Juan Carlos Zenteno, Martín Guzmán-Sánchez, Verónica Giordano-Herrera, Dalia Guadarrama-Vallejo, Narlly Ruíz-Quintero, Cristina Villanueva-Mendoza
Tietz syndrome and Waardenburg syndrome type 2A are allelic conditions caused by MITF mutations. Tietz syndrome is inherited in an autosomal dominant pattern and is characterized by congenital deafness and generalized skin, hair, and eye hypopigmentation, while Waardenburg syndrome type 2A typically includes variable degrees of sensorineural hearing loss and patches of de-pigmented skin, hair, and irides. In this paper, we report two unrelated families with MITF mutations. The first family showed an autosomal dominant pattern and variable expressivity...
September 8, 2016: American Journal of Medical Genetics. Part A
T von Braunmühl, D Hartmann, J K Tietze, D Cekovic, C Kunte, T Ruzicka, C Berking, E C Sattler
BACKGROUND: Optical coherence tomography (OCT) has become a valuable non-invasive tool in the in vivo diagnosis of non-melanoma skin cancer, especially of basal cell carcinoma (BCC). Due to an updated software-supported algorithm, a new en-face mode - similar to the horizontal en-face mode in high-definition OCT and reflectance confocal microscopy - surface-parallel imaging is possible which, in combination with the established slice mode of frequency domain (FD-)OCT, may offer additional information in the diagnosis of BCC...
August 31, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
Kerstin Lenk, Barbara Priwitzer, Laura Ylä-Outinen, Lukas H B Tietz, Susanna Narkilahti, Jari A K Hyttinen
BACKGROUND: Microelectrode array (MEA) is a widely used technique to study for example the functional properties of neuronal networks derived from human embryonic stem cells (hESC-NN). With hESC-NN, we can investigate the earliest developmental stages of neuronal network formation in the human brain. METHODS: In this paper, we propose an in silico model of maturating hESC-NNs based on a phenomenological model called INEX. We focus on simulations of the development of bursts in hESC-NNs, which are the main feature of neuronal activation patterns...
2016: Biomedical Engineering Online
Tobias Meinert, Olaf Tietz, Klaus J Palme, Alexander Rohrbach
Image quality in light-sheet fluorescence microscopy is strongly affected by the shape of the illuminating laser beam inside embryos, plants or tissue. While the phase of Gaussian or Bessel beams propagating through thousands of cells can be partly controlled holographically, the propagation of fluorescence light to the detector is difficult to control. With each scatter process a fluorescence photon loses information necessary for the image generation. Using Arabidopsis root tips we demonstrate that ballistic and diffusive fluorescence photons can be separated by analyzing the image spectra in each plane without a priori knowledge...
2016: Scientific Reports
Jana-Kristin Niemann, Thomas Alter, Greta Gölz, Erhard Tietze, Angelika Fruth, Wolfgang Rabsch, Christiane von Münchhausen, Roswitha Merle, Lothar Kreienbrock
The objectives of this study were to gather data on the occurrence of Salmonella (S.) enterica, Campylobacter spp. and Yersinia (Y.) enterocolitica along the pork production chain and to further analyze detected Salmonella isolates by additionally applying MLVA (multiple-locus variable-number tandem repeat analysis). In total, 336 samples were collected at primary production, slaughter and meat processing from five conventional fattening pig farms and one common slaughterhouse. At farm level, S. enterica, Campylobacter spp...
July 2016: Berliner und Münchener Tierärztliche Wochenschrift
Silvia M Tietz, Marcel Zwahlen, Neda Haghayegh Jahromi, Pascale Baden, Ivana Lazarevic, Gaby Enzmann, Britta Engelhardt
Considering the 3R rules of animal experimentation, we asked if refined scoring of experimental autoimmune encephalomyelitis (EAE) in mice improves documentation of clinical EAE allowing to perform a more powerful statistical analysis. Surprisingly, refined EAE scoring failed to improve statistical outcome comparing the overall disease courses between two groups of mice.
October 2016: European Journal of Immunology
Monika Dadrich, Nils H Nicolay, Paul Flechsig, Sebastian Bickelhaupt, Line Hoeltgen, Falk Roeder, Kai Hauser, Alexandra Tietz, Jürgen Jenne, Ramon Lopez, Manuel Roehrich, Ute Wirkner, Michael Lahn, Peter E Huber
Background : Radiotherapy (RT) is a mainstay for the treatment of lung cancer, but the effective dose is often limited by the development of radiation-induced pneumonitis and pulmonary fibrosis. Transforming growth factor β (TGFβ) and platelet-derived growth factor (PDGF) play crucial roles in the development of these diseases, but the effects of dual growth factor inhibition on pulmonary fibrosis development remain unclear. Methods : C57BL/6 mice were treated with 20 Gy to the thorax to induce pulmonary fibrosis...
May 2016: Oncoimmunology
Joanna Napp, Luis A Pardo, Franziska Hartung, Lutz F Tietze, Walter Stühmer, Frauke Alves
The Kv10.1 (Eag1) voltage-gated potassium channel represents a promising molecular target for novel cancer therapies or diagnostic purposes. Physiologically, it is only expressed in the brain, but it was found overexpressed in more than 70 % of tumours of diverse origin. Furthermore, as a plasma membrane protein, it is easily accessible to extracellular interventions. In this study we analysed the feasibility of the anti-Kv10.1 monoclonal antibody mAb62 to target tumour cells in vitro and in vivo and to deliver therapeutics to the tumour...
October 2016: European Biophysics Journal: EBJ
Ole Tietz, Jatinder Kaur, Atul Bhardwaj, Frank R Wuest
The cyclooxygenase-2 (COX-2) enzyme is overexpressed in a variety of cancers and mediates inflammatory processes that aid the growth and progression of malignancies. Three novel and selective fluorescent COX-2 inhibitors have been designed and synthesized on the basis of previously reported pyrimidine-based COX-2 inhibitors and the 7-nitrobenzofurazan fluorophore. In vitro evaluation of COX-1/COX-2 isozyme inhibition identified N-(2-((7-nitro-benzo[c][1,2,5]oxadiazol-4-yl)amino)propyl)-4-[4-(methylsulfonyl)phenyl]-6-(trifluoro-methyl)-pyrimidin-2-amine (6) as a novel potent and selective COX-2 inhibitor (IC50 = 1...
July 26, 2016: Organic & Biomolecular Chemistry
Lisa Ann Gerdes, Kathrin Held, Eduardo Beltrán, Carola Berking, Jörg C Prinz, Andreas Junker, Julia K Tietze, Birgit Ertl-Wagner, Andreas Straube, Tania Kümpfel, Klaus Dornmair, Reinhard Hohlfeld
We investigated a patient who developed multiple sclerosis (MS) during treatment with the CTLA4-blocking antibody ipilimumab for metastatic melanoma. Initially he showed subclinical magnetic resonance imaging (MRI) changes (radiologically isolated syndrome). Two courses of ipilimumab were each followed by a clinical episode of MS, 1 of which was accompanied by a massive increase of MRI activity. Brain biopsy confirmed active, T-cell type MS. Quantitative next generation sequencing of T-cell receptor genes revealed distinct oligoclonal CD4(+) and CD8(+) T-cell repertoires in the primary melanoma and cerebrospinal fluid...
August 2016: Annals of Neurology
Kay Wilhelm, Viola Korczak, Tad Tietze, Prasuna Reddy
Rates of self-harm in Australia are increasing and constitute a concerning public health issue. Although there are standard treatment pathways for physical complaints, such as headache, abdominal pain and chest pain, in Emergency Medicine, there is no national pathway for self-harm or other psychiatric conditions that present to the emergency department. Herein we outline the difference between clinical practice guidelines and clinical pathways, discuss pathways we have identified on self-harm in Australia and overseas and discuss their applicability to the Australian context and the next steps forward in addressing this public health issue...
June 23, 2016: Australian Health Review: a Publication of the Australian Hospital Association
Erhard Næss-Schmidt, Anna Tietze, Jakob Udby Blicher, Mikkel Petersen, Irene K Mikkelsen, Pierrick Coupé, José V Manjón, Simon Fristed Eskildsen
PURPOSE: In both structural and functional MRI, there is a need for accurate and reliable automatic segmentation of brain regions. Inconsistent segmentation reduces sensitivity and may bias results in clinical studies. The current study compares the performance of publicly available segmentation tools and their impact on diffusion quantification, emphasizing the importance of using recently developed segmentation algorithms and imaging techniques. METHODS: Four publicly available, automatic segmentation methods (volBrain, FSL, FreeSurfer and SPM) are compared to manual segmentation of the thalamus and hippocampus imaged with a recently proposed T1-weighted MRI sequence (MP2RAGE)...
June 20, 2016: International Journal of Computer Assisted Radiology and Surgery
Daniel Tietze, Enrico Leipold, Pascal Heimer, Miriam Böhm, Wadim Winschel, Diana Imhof, Stefan H Heinemann, Alesia A Tietze
No abstract text is available yet for this article.
September 2016: Biochimica et Biophysica Acta
Markus V Heppt, Thomas K Eigentler, Katharina C Kähler, Rudolf A Herbst, Daniela Göppner, Thilo Gambichler, Jens Ulrich, Edgar Dippel, Carmen Loquai, Beatrice Schell, Bastian Schilling, Susanne G Schäd, Erwin S Schultz, Fanny Matheis, Julia K Tietze, Carola Berking
Growing evidence suggests that concurrent loco-regional and systemic treatment modalities may lead to synergistic anti-tumor effects in advanced melanoma. In this retrospective multicenter study, we evaluate the use of electrochemotherapy (ECT) combined with ipilimumab or PD-1 inhibition. We investigated patients with unresectable or metastatic melanoma who received the combination of ECT and immune checkpoint blockade for distant or cutaneous metastases within 4 weeks. Clinical and laboratory data were collected and analyzed with respect to safety and efficacy...
August 2016: Cancer Immunology, Immunotherapy: CII
Jonathan K Williams, Daniel Tietze, Myungwoon Lee, Jun Wang, Mei Hong
Together with the influenza A virus, influenza B virus causes seasonal flu epidemics. The M2 protein of influenza B (BM2) forms a tetrameric proton-conducting channel that is important for the virus lifecycle. BM2 shares little sequence homology with AM2, except for a conserved HxxxW motif in the transmembrane (TM) domain. Unlike AM2, no antiviral drugs have been developed to block the BM2 channel. To elucidate the proton-conduction mechanism of BM2 and to facilitate the development of BM2 inhibitors, we have employed solid-state NMR spectroscopy to investigate the conformation, dynamics, and hydration of the BM2 TM domain in lipid bilayers...
July 6, 2016: Journal of the American Chemical Society
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