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Rif xpert for tuberculosis

Guirong Wang, Weijie Dong, Tinglong Lan, Jun Fan, Kai Tang, Yuan Li, Guangxuan Yan, Guanglu Jiang, Yifeng Ma, Yuanyuan Shang, Shibing Qin, Hairong Huang
Early diagnosis of spinal tuberculosis (TB) is hampered by the flaws of conventional tests. The aim of this study was to assess the value of new and existing molecular tests in a prospective, head-to-head cohort study. Specimens were consecutively collected from spinal TB suspects in four hospitals in Beijing, China. Smear, culture, histopathology, Xpert MTB/RIF (Xpert), and MeltPro TB assays were performed in parallel using the same specimen from each patient. Drug-susceptibility testing (DST) was conducted on the isolates recovered...
June 20, 2018: Emerging Microbes & Infections
B W P Reeve, S M McFall, R Song, R Warren, K R Steingart, G Theron
SETTING: Eliminating tuberculosis in high-burden settings requires improved diagnostic capacity. Important tests such as Xpert® MTB/RIF and culture are often performed at centralised laboratories that are geographically distant from the point of specimen collection. Preserving specimen integrity during transportation, which could affect test performance, is challenging. OBJECTIVE: To conduct a systematic review of commercial products for specimen preservation for a World Health Organization technical consultation...
July 1, 2018: International Journal of Tuberculosis and Lung Disease
Claudia Elena Guajardo-Lara, Martha Idalia Saldaña-Ramírez, Nayelly Nohemí Hernández-Galván, Miguel Adrián Dimas-Adame, Juan Jacobo Ayala-Gaytán, Salvador Bruno Valdovinos-Chávez
Background: Tuberculosis (TB) is still a threat to public health; in 2014 caused 1.5 million deaths worldwide; in hospitals where the prevalence of TB is low, it is appropriate to evaluate the effectiveness of tests to diagnose it. Methods: We reviewed reports of airway clinical specimens sent for studies of tuberculosis to the microbiology laboratory from Tec Salud System private hospitals (400 beds) in the metropolitan area of ​​Monterrey, NL, from May 2012 to December 2015...
March 2018: Revista Médica del Instituto Mexicano del Seguro Social
Godfrey Bigogo, Kevin Cain, Diana Nyole, Geoffrey Masyongo, Joshua Auko Auko, Newton Wamola, Albert Okumu, Janet Agaya, Joel Montgomery, Martien Borgdorff, Deron Burton
BACKGROUND: Tuberculosis (TB) case finding is an important component of TB control because it can reduce transmission of Mycobacterium tuberculosis (MTB) through prompt detection and treatment of infectious patients. METHODS: Using population-based infectious disease surveillance (PBIDS) platforms with links to health facilities in Kenya we implemented intensified TB case finding in the community and at the health facilities, as an adjunct to routine passive case finding conducted by the national TB program...
June 7, 2018: BMC Infectious Diseases
Sarah Wood Pallas, Marissa Courey, Chhaily Hy, Wm Perry Killam, Dora Warren, Brittany Moore
BACKGROUND: The Xpert® MTB/RIF (Xpert) test has been shown to be effective and cost-effective for diagnosing tuberculosis (TB) under conditions with high HIV prevalence and HIV-TB co-infection but less is known about Xpert's cost in low HIV prevalence settings. Cambodia, a country with low HIV prevalence (0.7%), high TB burden, and low multidrug-resistant (MDR) TB burden (1.4% of new TB cases, 11% of retreatment cases) introduced Xpert into its TB diagnostic algorithms for people living with HIV (PLHIV) and people with presumptive MDR TB in 2012...
June 4, 2018: Applied Health Economics and Health Policy
Jin Shi, Wenzhu Dong, Yifeng Ma, Qian Liang, Yuanyuan Shang, Fen Wang, Hairong Huang, Yu Pang
GeneXpert MTB/RIF (Xpert) assay has been endorsed for the diagnosis of pulmonary TB due to its high sensitivity and specificity for culture positive TB. There is no doubt that Xpert could not be more sensitive than mycobacterial culture, while the positive rate of Xpert among sputum samples was higher than that of mycobacterial culture in our laboratory. We therefore carried out a prospective study to determine a potential explanation for this unexpected result regarding the clinical use of Xpert. Overall, a total of 558 patients meeting inclusion criteria were enrolled in final analysis between August 2017 and September 2017 in Beijing Chest Hospital...
2018: BioMed Research International
C Q Yang, X Y Liu, R H Du, T Z Cao, X Y Dai
Objective: To evaluate the accuracy of Xpert MTB/RIF assay in the diagnosis of cervical tuberculous lymphadenitis. Method: A total of 160 patients with cervical lymph node tuberculosis confirmed by pathology in Wuhan Pulmonary Hospital between January 2015 and June 2016 were enrolled. Cervical lymph node biopsy tissue specimens from these patients were collected and tested with acid-fast bacilli smear, TB-DNA assays, culture, and Xpert Mtb/RIF, respectively. The results were analyzed using SPSS 17.0 statistical software...
September 5, 2017: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Marilda Casela, Silvânia Maria Andrade Cerqueira, Thais de Oliveira Casela, Mariana Araújo Pereira, Samanta Queiroz Dos Santos, Franco Andres Del Pozo, Songeli Menezes Freire, Eliana Dias Matos
OBJECTIVE: To evaluate the impact of the use of the molecular test for Mycobacterium tuberculosis and its resistance to rifampin (Xpert MTB/RIF), under routine conditions, at a referral hospital in the Brazilian state of Bahia. METHODS: This was a descriptive study using the database of the Mycobacteriology Laboratory of the Octávio Mangabeira Specialized Hospital, in the city of Salvador, and georeferencing software. We evaluated 3,877 sputum samples collected from symptomatic respiratory patients, under routine conditions, between June of 2014 and March of 2015...
April 2018: Jornal Brasileiro de Pneumologia: Publicaça̋o Oficial da Sociedade Brasileira de Pneumologia e Tisilogia
Ambarish Dutta, Sarthak Pattanaik, Rajendra Choudhury, Pritish Nanda, Suvanand Sahu, Rajendra Panigrahi, Bijaya K Padhi, Krushna Chandra Sahoo, P R Mishra, Pinaki Panigrahi, Daisy Lekharu, Robert H Stevens
BACKGROUND: Migrant labourers living in the slums of urban and industrial patches across India make up a key sub-population so far controlling Tuberculosis (TB) in the country is concerned. This is because many TB patients from these communities- remain under reached by the Revised National Tuberculosis Control Programme (RNTCP) of India. This marginalized community usually seeks early-stage healthcare from "friendly neighbourhood" non-formal health providers (NFHPs). Because, RNTCP has limited capacity to involve the NFHPs, an implementation research project was conceived, whereby an external partner would engage with the NFHPs to enable them to identify early TB symptomatics from this key sub-population who would be then tested using Xpert MTB/RIF technology...
2018: PloS One
Joshua Kostera, Gregor Leckie, Klara Abravaya, Hong Wang
Introduction: The Abbott RealTime MTB RIF/INH Resistance Assay (RT MTB RIF/INH) is an assay for the detection of rifampicin (RIF)- and/or isoniazid (INH)-resistant Mycobacterium tuberculosis (MTB). The assay can be used to test sputum, bronchial alveolar lavage, and N-Acetyl-L-Cysteine (NALC)/NaOH pellets prepared from these samples. The assay can be used in direct testing mode, or in reflex mode following a MTB positive result produced by its companion assay, Abbott RT MTB. Methods: In this study, the direct testing mode was used to test paired sputum and NALC/NaOH pellets prepared from sputum collected from Bangladesh TB patients...
2018: Infection and Drug Resistance
Cinara Silva Feliciano, Evangelina Inacio Namburete, Jéssica Rodrigues Plaça, Kamila Peronni, Anzaan Dippenaar, Robin Mark Warren, Wilson Araújo Silva, Valdes Roberto Bollela
BACKGROUND: The fast and accurate diagnosis of drug-resistant tuberculosis (DR-TB) is critical to reducing the spread of disease. Although commercial genotypic drug-susceptibility tests (DST) are close to the goal, they are still not able to detect all relevant DR-TB related mutations. Whole genome sequencing (WGS) allows better comprehension of DR-TB with a great discriminatory power. We aimed to evaluate WGS in M. tuberculosis isolates compared with phenotypic and genotypic DST. METHODS: This cross-sectional study evaluated 30 isolates from patients with detected DR-TB in Brazil and Mozambique...
May 2018: Tuberculosis
Andrea Rachow, Elmar Saathoff, Bariki Mtafya, Daniel Mapamba, Chacha Mangu, Gabriel Rojas-Ponce, Nyanda E Ntinginya, Martin Boeree, Norbert Heinrich, Stephen H Gillespie, Michael Hoelscher
The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin ). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs...
May 2018: Tuberculosis
I Khan, N Ndubuka, K Stewart, V McKinney, I Mendez
Background: Saskatchewan is a province of over one million people and over 13% are Indigenous peoples, many of whom live on reserve lands. Despite continued efforts, access to health care remains a significant challenge for these Indigenous people, especially those in the North. Objective: To address this challenge, Saskatchewan's health care providers have been incorporating the use of technology for various health services. This paper describes various ways technology has been used in First Nations communities in Saskatchewan...
June 1, 2017: Canada Communicable Disease Report, Relevé des Maladies Transmissibles Au Canada
Thania Luiza de Andrade Sieiro, Rafaela Baroni Aurílio, Elizabeth Cristina C Soares, Silvia S Chiang, Clemax Couto Sant Anna
INTRODUCTION: The teste rápido molecular para tuberculose (TRM-TB) was introduced in 2014 in Brazil for tuberculosis screening. However, its role in adolescents in Brazil has not been studied. METHODS: A descriptive study of adolescents with suspected tuberculosis using National Laboratory software. RESULTS: Of 852 (15.4%) suspected cases, 131 were positive by TRM-TB and 2% were resistant to rifampicin. Among TRM-TB-positive cases, 105 (91...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
Patrick Orikiriza, Margaret Nansumba, Dan Nyehangane, Mathieu Bastard, Ivan Taremwa Mugisha, Denis Nansera, Juliet Mwanga-Amumpaire, Yap Boum, Elias Kumbakumba, Maryline Bonnet
The Xpert MTB/RIF assay is a major advance for diagnosis of tuberculosis (TB) in high-burden countries but is limited in children by their difficulty to produce sputum. We investigated TB in sputum and stool from children with the aim of improving paediatric TB diagnosis. A prospective cohort of children with presumptive TB, provided two sputum or induced sputum at enrolment in a regional referral hospital in Uganda. Stool was collected from those started on TB treatment. All specimen were tested for Xpert MTB/RIF, mycobacteria growth indicator tube (MGIT), Lowenstein Jensen cultures and microscopy (except stool)...
May 8, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Guirong Wang, Shuqi Wang, Guanglu Jiang, Yuhong Fu, Yuanyuan Shang, Hairong Huang
Background: Due to the non-homogeneity of specimens collected from tuberculosis (TB) suspects, repeated Xpert MTB/RIF (Xpert) may have potential clinical benefits. Incremental cost-effectiveness was analyzed for the second Xpert assay to detect Mycobacterium tuberculosis (Mtb) and rifampicin (RIF) resistance. Methods: Specimens were collected from 1,063 pulmonary TB (PTB) and 398 extrapulmonary TB (EPTB) suspects, who had two Xpert tests sequentially within one week...
March 2018: Journal of Thoracic Disease
Andrew R DiNardo, Alexander W Kay, Gugu Maphalala, Nadine M Harris, Celia Fung, Godwin Mtetwa, Pilar Ustero, Sindisiwe Dlamini, Ngan Ha, Edward A Graviss, Rojelio Mejia, Anna M Mandalakas
A quantifiable, stool-based, Mycobacterium tuberculosis ( Mtb ) test has potential complementary value to respiratory specimens. Limit of detection (LOD) was determined by spiking control stool. Clinical test performance was evaluated in a cohort with pulmonary tuberculosis (TB) ( N = 166) and asymptomatic household TB child contacts ( N = 105). Stool-quantifiable polymerase chain reaction (qPCR) results were compared with sputum acid-fast bacilli (AFB) microscopy, GeneXpert MTB/RIF (Xpert MTB/RIF), and cultures...
April 23, 2018: American Journal of Tropical Medicine and Hygiene
Y Merid, Y Woldeamanuel, M Abebe, D G Datiko, T Hailu, G Habtamu, G Assefa, R R Kempker, H M Blumberg, A Aseffa
SETTING: Hawassa Prison, Southern Region of Ethiopia. OBJECTIVE: To determine the burden of pulmonary tuberculosis (TB) using active case finding among prisoners. DESIGN: In this cross-sectional study, prisoners were screened for TB using a symptom screen. Those with cough of 2 weeks had spot and morning sputum samples collected for acid-fast bacilli (AFB) smear microscopy and molecular diagnostic testing (Xpert® MTB/RIF). RESULTS: Among 2068 prisoners, 372 (18%) had a positive cough screen...
May 1, 2018: International Journal of Tuberculosis and Lung Disease
Xiaofu Pan, Shoufeng Yang, Margaret A Deighton, Yue Qu, Liang Hong, Feifei Su
Introduction: The Xpert MTB/RIF is recommended by the World Health Organization as a first line rapid test for the diagnosis of pulmonary tuberculosis (TB); however, China does not routinely use this test, partially due to the lack of a sufficient number of systematic evaluations of this assay in local patients. The aims of this study were to comprehensively assess the diagnostic performance of Xpert MTB/RIF, either alone or in combination with conventional assays for the diagnosis of pulmonary TB in adult Chinese patients...
2018: Frontiers in Microbiology
W Ng'ambi, S Gugsa, H Tweya, B Girma, H Kanyerere, I Dambe, Y Babaye, J Mpunga, S Phiri
Setting: Public health facilities providing tuberculosis (TB) and human immunodeficiency virus (HIV) services in Malawi. Objectives: Using routinely collected health service delivery data to describe trends in HIV ascertainment and use of the Xpert® MTB/RIF assay to diagnose TB among HIV-positive presumptive TB cases. Design: This was an implementation study of presumptive TB cases who sought care from 21 facilities between April 2014 and June 2016. Descriptive statistics were used to summarise patient, facility and service level characteristics...
December 21, 2017: Public Health Action
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