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proteus mirabilis in urine

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https://www.readbyqxmd.com/read/28150092/antimicrobial-activity-study-of-triclosan-loaded-wbpu-on-proteus-mirabilis-in-vitro
#1
Ye Tian, Zhongyu Jian, Jianzhong Wang, Wei He, Qinyu Liu, Kunjie Wang, Hong Li, Hong Tan
OBJECTIVES: To evaluate the antimicrobial activity study of triclosan-loaded waterborne polyurethanes (WBPU) on Proteus mirabilis in vitro. MATERIALS AND METHODS: Inhibition zone assays on petri plates with triclosan-loaded WBPU samples were used to test its antimicrobial activity on Proteus mirabilis. Models of the catheterized bladder supplied with artificial urine infected with Proteus mirabilis were employed to confirm the antimicrobial activity of triclosan-loaded WBPU...
February 1, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28031261/copper-is-a-host-effector-mobilized-to-urine-during-urinary-tract-infection-to-impair-bacterial-colonization
#2
Amanda N Hyre, Kylie Kavanagh, Nancy D Kock, George L Donati, Sargurunathan Subashchandrabose
Urinary tract infection (UTI) is a major global infectious disease affecting millions of people annually. Human urinary copper (Cu) content is elevated during UTI caused by uropathogenic E. coli (UPEC). UPEC upregulate the expression of Cu efflux genes during clinical UTI in patients as an adaptive response to host-derived Cu. Whether Cu is mobilized to urine as a host response to UTI and its role in protection against UTI remain unresolved. To address these questions, we tested the hypothesis that Cu is a host effector mobilized to urine during UTI to limit bacterial growth...
December 28, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27895127/the-pathogenic-potential-of-proteus-mirabilis-is-enhanced-by-other-uropathogens-during-polymicrobial-urinary-tract-infection
#3
Chelsie E Armbruster, Sara N Smith, Alexandra O Johnson, Valerie DeOrnellas, Kathryn A Eaton, Alejandra Yep, Lona Mody, Weisheng Wu, Harry L T Mobley
Urinary catheter use is prevalent in health care settings, and polymicrobial colonization by urease-positive organisms, such as Proteus mirabilis and Providencia stuartii, commonly occurs with long-term catheterization. We previously demonstrated that coinfection with P. mirabilis and P. stuartii increased overall urease activity in vitro and disease severity in a model of urinary tract infection (UTI). In this study, we expanded these findings to a murine model of catheter-associated UTI (CAUTI), delineated the contribution of enhanced urease activity to coinfection pathogenesis, and screened for enhanced urease activity with other common CAUTI pathogens...
February 2017: Infection and Immunity
https://www.readbyqxmd.com/read/27856593/genome-sequences-of-two-multidrug-resistant-proteus-mirabilis-strains-harboring-ctx-m-65-isolated-from-malaysia
#4
Choo Yee Yu, Geik Yong Ang, Yun Fong Ngeow, Kok Keng Tee, Wai-Fong Yin, Kok-Gan Chan
Proteus mirabilis is an opportunistic nosocomial pathogen that is commonly associated with urinary tract infections. Here, we present draft genome sequences of two multidrug-resistant P. mirabilis strains, isolated from urine samples in Malaysia, that harbored a CTX-M-type extended-spectrum β-lactamase-encoding gene, as well as a repertoire of other antimicrobial-resistant determinants.
November 17, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27847969/-migration-speed-of-uropathogens-determination-in-the-ureteral-splint-in-a-practice-like-in-vitro-model
#5
H Braunwarth, F H H Brill, J Steinmann, D Hegeholz, W Droste
BACKGROUND: A urostomy with an ileum conduit or a skin fistula leads to a high infection risk for the kidneys and the ureter of the patient. Therefore, the prevention of retrograde colonization of the artificial drain (splint) with e. g. contaminated urine is the most important objective of infection prevention measurements. We performed an in vitro experiment to determine the migration speed of clinically relevant bacteria in a commercially available splint catheter system. METHODS: The migration speed of bacteria in commonly used splint catheters was determined in a practice-like in vitro model...
February 2017: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/27807154/detection-of-an-escherichia-coli-sequence-type-167-strain-with-two-tandem-copies-of-blandm-1-in-the-chromosome
#6
Ping Shen, Maoli Yi, Ying Fu, Zhi Ruan, Xiaoxing Du, Yunsong Yu, Xinyou Xie
New Delhi metallo-β-lactamase-1 (NDM-1)-producing Enterobacteriaceae has disseminated rapidly throughout the world and poses an urgent threat to public health. Previous studies confirmed that the blaNDM-1 gene is typically carried in plasmids but rarely in chromosome. We discovered a multidrug-resistant Escherichia coli strain Y5, originating from a urine sample and containing the blaNDM-1 gene, which did not transfer by either conjugation or electrotransformation. We confirmed the possibility of its chromosome location by S1-pulsed-field gel electrophoresis (PFGE) and XbaI-PFGE, followed by Southern blotting...
January 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27749743/method-for-bacterial-growth-and-ammonia-production-and-effect-of-inhibitory-substances-in-disposable-absorbent-hygiene-products
#7
Ulla Forsgren-Brusk, Birgitta Yhlen, Marie Blomqvist, Peter Larsson
PURPOSE: The purpose of this study was to evaluate a pragmatic laboratory method to provide a technique for developing incontinence products better able to reduce malodor when used in the clinical setting. METHODS: Bacterial growth and bacterially formed ammonia in disposable absorbent incontinence products was measured by adding synthetic urine inoculated with bacteria to test samples cut from the crotch area of the product. The inhibitory effect's of low pH (4...
January 2017: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/27642801/role-of-interspecies-interactions-in-dual-species-biofilms-developed-in-vitro-by-uropathogens-isolated-from-polymicrobial-urinary-catheter-associated-bacteriuria
#8
E M Galván, C Mateyca, L Ielpi
Most catheter-associated urinary tract infections are polymicrobial. Here, uropathogen interactions in dual-species biofilms were studied. The dual-species associations selected based on their prevalence in clinical settings were Klebsiella pneumoniae-Escherichia coli, E. coli-Enterococcus faecalis, K. pneumoniae-E. faecalis, and K. pneumoniae-Proteus mirabilis. All species developed single-species biofilms in artificial urine. The ability of K. pneumoniae to form biofilms was not affected by E. coli or E. faecalis co-inoculation, but was impaired by P...
October 2016: Biofouling
https://www.readbyqxmd.com/read/27569886/prevalence-and-antimicrobial-resistance-pattern-of-bacterial-strains-isolated-from-patients-with-urinary-tract-infection-in-messalata-central-hospital-libya
#9
Mahmoud A Mohammed, Tarig M S Alnour, Osama M Shakurfo, Mariam M Aburass
OBJECTIVES: To investigate the prevalence of urinary tract infection among patients at Messalata Central Hospital, Libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. METHODS: A total number of 1153 urine samples were collected from patients, who attended daily to Messalata Central Hospital, Libya, in a study extended for one year. Antimicrobial susceptibility testing and isolates typing were done using Phoenix BD (BD diagnostic)...
August 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27550502/whole-cell-proteus-mirabilis-urease-inhibition-by-aminophosphinates-for-the-control-of-struvite-formation
#10
Ewa Grela, Anna Dziełak, Katarzyna Szydłowska, Artur Mucha, Paweł Kafarski, Agnieszka Monika Grabowiecka
The study evaluated the in vitro impact of a series of aminophosphinic urease inhibitors on Proteusmirabilis. The group of compounds comprised structurally diverse analogues of diamidophosphate built on an N-C-P scaffold. The influence of urease inhibition on urea-splitting activity was assessed by whole-cell pH-static kinetic measurements. The potential to prevent struvite formation was determined by monitoring changes in pH and ionic composition of artificial urine medium during P. mirabilis growth. The most active compounds exhibited stronger positive effect on urine stability than the acknowledged inhibitor acetohydroxamic acid...
October 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27469376/classification-of-proteus-penneri-lipopolysaccharides-into-core-region-serotypes
#11
Agata Palusiak
The frequency of P. penneri isolation from hospital patients, mostly from urine and wounds, keeps on growing, and numerous isolates are multi-drug resistant. P. penneri rods produce lipopolysaccharide (LPS), which may lead to the septic shock. Until now, O-specific polysaccharide has been the best structurally and serologically characterized region of P. penneri LPS. It is worth having an insight into the serological specificity of both poly- and oligosaccharide parts of P. penneri LPS. The P. penneri core region is less structurally diverse than OPS, but still, among other enterobacterial LPS core regions, it is characterized by structural variability...
December 2016: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/27383862/pathology-image-of-the-month-recurrent-urinary-tract-infections-in-a-48-year-old-female-hyperintensities-in-the-globus-pallidi
#12
Ellen E Connor, Anthony Vinson, Ritu Bhalla, Robin R McGoey
A 48-year-old female presented to her physician complaining of intermittent lower abdominal pain radiating to the right lower back. At the time of presentation, she was afebrile and denied any urinary symptoms such as dysuria, frequency or urgency. Physical exam was unremarkable, other than obesity, and her abdominal exam was noncontributory. Medical history, however, was notable for recurrent, Proteus mirabilis culture-proven urinary tract infections requiring antibiotic treatment for the prior three years along with hypertension, uterine fibroids post hysterectomy, diabetes and asthma...
March 2016: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
https://www.readbyqxmd.com/read/27341161/dissemination-of-a-multidrug-resistant-vim-1-and-cmy-99-producing-proteus-mirabilis-clone-in-bulgaria
#13
Rumyana Markovska, Ines Schneider, Emma Keuleyan, Dobrinka Ivanova, Magdalena Lesseva, Temenuga Stoeva, Mariya Sredkova, Adolf Bauernfeind, Ivan Mitov
The aim of this study was to analyze the beta-lactamases and the molecular epidemiology of 19 clinically significant isolates of Proteus mirabilis with decreased susceptibility to imipenem, which have been collected from seven hospitals, located in different Bulgarian towns (Sofia, Varna, and Pleven). The isolates were obtained from blood, urine, tracheal and wound specimens. One additional isolate from hospital environment was included. Susceptibility testing, conjugation experiments, and plasmid replicon typing were carried out...
June 24, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27312379/evaluation-of-the-in-vitro-growth-of-urinary-tract-infection-causing-gram-negative-and-gram-positive-bacteria-in-a-proposed-synthetic-human-urine-shu-medium
#14
Deepak S Ipe, Glen C Ulett
Bacteriuria is a hallmark of urinary tract infection (UTI) and asymptomatic bacteriuria (ABU), which are among the most frequent infections in humans. A variety of gram-negative and gram-positive bacteria are associated with these infections but Escherichia coli contributes up to 80% of cases. Multiple bacterial species including E. coli can grow in human urine as a means to maintain colonization during infections. In vitro bacteriuria studies aimed at modeling microbial growth in urine have utilized various compositions of synthetic human urine (SHU) and a Composite SHU formulation was recently proposed...
August 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27091004/fimbriae-have-distinguishable-roles-in-proteus-mirabilis-biofilm-formation
#15
Paola Scavone, Victoria Iribarnegaray, Ana Laura Caetano, Geraldine Schlapp, Steffen Härtel, Pablo Zunino
Proteus mirabilis is one of the most common etiological agents of complicated urinary tract infections, especially those associated with catheterization. This is related to the ability of P. mirabilis to form biofilms on different surfaces. This pathogen encodes 17 putative fimbrial operons, the highest number found in any sequenced bacterial species so far. The present study analyzed the role of four P. mirabilis fimbriae (MR/P, UCA, ATF and PMF) in biofilm formation using isogenic mutants. Experimental approaches included migration over catheter, swimming and swarming motility, the semiquantitative assay based on adhesion and crystal violet staining, and biofilm development by immunofluorescence and confocal microscopy...
2016: Pathogens and Disease
https://www.readbyqxmd.com/read/27090825/cranberry-derivatives-enhance-biofilm-formation-and-transiently-impair-swarming-motility-of-the-uropathogen-proteus-mirabilis-hi4320
#16
Che O'May, Olivier Amzallag, Karim Bechir, Nathalie Tufenkji
Proteus mirabilis is a major cause of catheter-associated urinary tract infection (CAUTI), emphasizing that novel strategies for targeting this bacterium are needed. Potential targets are P. mirabilis surface-associated swarming motility and the propensity of these bacteria to form biofilms that may lead to catheter blockage. We previously showed that the addition of cranberry powder (CP) to lysogeny broth (LB) medium resulted in impaired P. mirabilis swarming motility over short time periods (up to 16 h). Herein, we significantly expanded on those findings by exploring (i) the effects of cranberry derivatives on biofilm formation of P...
June 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27014523/comparison-of-hemagglutination-and-hemolytic-activity-of-various-bacterial-clinical-isolates-against-different-human-blood-groups
#17
Rajkumar Hrv, Ramakrishna Devaki, Venkataramana Kandi
Among the various pathogenic determinants shown by microorganisms hemagglutination and hemolysin production assume greater significance in terms of laboratory identification. This study evaluated the hemagglutination and hemolytic activity of various bacterial isolates against different blood groups. One hundred and fifty bacterial strains, isolated from clinical specimens like urine, pus, blood, and other body fluids were tested for their hemagglutinating and hemolytic activity against human A, B, AB, and O group red blood cells...
2016: Curēus
https://www.readbyqxmd.com/read/26976871/urinary-concentrations-and-antibacterial-activity-of-bal30072-a-novel-siderophore-monosulfactam-against-uropathogens-after-intravenous-administration-in-healthy-subjects
#18
Marion Straubinger, Holger Blenk, Kurt G Naber, Florian M E Wagenlehner
This annex study to a phase 1 study aimed to correlate urinary concentrations and bactericidal titers (UBTs) of BAL30072, a novel siderophore monosulfactam, in healthy subjects in order to evaluate which dosage of BAL30072 should be investigated in a clinical study on complicated urinary tract infection (UTI). Three cohorts of a total of 19 healthy male subjects were included in the add-on study and received the following BAL30072 dosages. The 1st cohort received 1 g once a day (q.d.) intravenously (i.v.) (1 h) on day 1 and 1 g thrice daily (t...
June 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/26954694/molecular-diagnosis-of-urinary-tract-infections-by-semi-quantitative-detection-of-uropathogens-in-a-routine-clinical-hospital-setting
#19
Anneke van der Zee, Lieuwe Roorda, Gerda Bosman, Jacobus M Ossewaarde
BACKGROUND: The objective of our study was the development of a semi-quantitative real-time PCR to detect uropathogens. Two multiplex PCR reactions were designed to detect Escherichia coli, Klebsiella spp., Enterobacter spp., Citrobacter spp., Proteus mirabilis, Enterococcus faecalis, and Pseudomonas aeruginosa. 16S based PCR was performed in parallel to detect Gram-positive and Gram-negative bacteria. Firstly to identify non-targeted agents of infection in the same urine specimen, and secondly to quantify background flora...
2016: PloS One
https://www.readbyqxmd.com/read/26953206/ureolytic-biomineralization-reduces-proteus-mirabilis-biofilm-susceptibility-to-ciprofloxacin
#20
Xiaobao Li, Nanxi Lu, Hannah R Brady, Aaron I Packman
Ureolytic biomineralization induced by urease-producing bacteria, particularly Proteus mirabilis, is responsible for the formation of urinary tract calculi and the encrustation of indwelling urinary catheters. Such microbial biofilms are challenging to eradicate and contribute to the persistence of catheter-associated urinary tract infections, but the mechanisms responsible for this recalcitrance remain obscure. In this study, we characterized the susceptibility of wild-type (ure+) and urease-negative (ure-) P...
May 2016: Antimicrobial Agents and Chemotherapy
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