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https://www.readbyqxmd.com/read/28706153/inhibitor-of-growth-protein-4-interacts-with-beclin-1-and-represses-autophagy
#1
Valentina Sica, José Manuel Bravo-San Pedro, Guo Chen, Guillermo Mariño, Sylvie Lachkar, Valentina Izzo, Maria Chiara Maiuri, Mireia Niso-Santano, Guido Kroemer
Beclin 1 (BECN1) is a multifunctional protein that activates the pro-autophagic class III phosphatidylinositol 3-kinase (PIK3C3, best known as VPS34), yet also interacts with multiple negative regulators. Here we report that BECN1 interacts with inhibitor of growth family member 4 (ING4), a tumor suppressor protein that is best known for its capacity to interact with the tumor suppressor protein p53 (TP53) and the acetyltransferase E1A binding protein p300 (EP300). Removal of TP53 or EP300 did not affect the BECN1/ING4 interaction, which however was lost upon culture of cells in autophagy-inducing, nutrient free conditions...
July 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28677541/generation-of-induced-pluripotent-stem-cell-ipsc-line-from-a-36-year-old-charcot-marie-tooth-disease-patient-with-gjb1-mutation-cmtx
#2
Daryeon Son, Phil Jun Kang, Wonjin Yun, Seungkwon You
Charcot-Marie-Tooth disease (CMTX) is inherited neurological disorder caused by gap junction beta 1 gene (GJB1) mutation. We generated induced pluripotent stem cell (iPSC) line from 36-year-old CMTX disease patient by electroporation of skin fibroblasts with episomal vectors encoding OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA-p53. Established iPSCs expressed various pluripotency markers, had differentiation potential of three germ layers in vitro, had normal karyotype and retained GJB1 mutation. This CMT patient-derived iPSC line could be useful in vitro tool for CMTX research as disease modeling and drug development...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28677373/commitment-of-protein-p53-and-amyloid-beta-peptide-a%C3%AE-in-aging-of-human-cerebellum
#3
Danuta Maślińska, Milena Laure-Kamionowska, Dariusz Szukiewicz, Sławomir Maśliński, Krystyna Księżopolska-Orłowska
Protein p53 is known to induce the cell cycle arrest and apoptosis in response to a variety of cellular distress signals and DNA damage. A recent study has demonstrated that in blood cells of aging subjects, p53 may induce early pathological changes that precede the amyloidogenic cascade. However, it is not clear whether p53 participates in the local deposition of amyloid-beta peptide (Aβ) in the nerve tissue of normal aging subjects. Therefore, in the present study, we analyse the distribution of both (Aβ and p53) proteins in the cerebellum of individuals without any history of dementia or other neurological illness who died suddenly in traffic accidents...
2017: Folia Neuropathologica
https://www.readbyqxmd.com/read/28673316/somatic-loss-of-estrogen-receptor-beta-and-p53-synergize-to-induce-breast-tumorigenesis
#4
Igor Bado, Fotis Nikolos, Gayani Rajapaksa, Wanfu Wu, Jessica Castaneda, Savitri Krishnamurthy, Paul Webb, Jan-Åke Gustafsson, Christoforos Thomas
BACKGROUND: Upregulation of estrogen receptor beta (ERβ) in breast cancer cells is associated with epithelial maintenance, decreased proliferation and invasion, and a reduction in the expression of the receptor has been observed in invasive breast tumors. However, proof of an association between loss of ERβ and breast carcinogenesis is still missing. METHODS: To study the role of ERβ in breast oncogenesis, we generated mouse conditional mutants with specific inactivation of ERβ and p53 in the mammary gland epithelium...
July 3, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/28656629/p53-induced-mir-30e-5p-inhibits-colorectal-cancer-invasion-and-metastasis-by-targeting-itga6-and-itgb1
#5
Sara Laudato, Nitin Patil, Mohammed L Abba, Joerg H Leupold, Axel Benner, Timo Gaiser, Alexander Marx, Heike Allgayer
The tumor suppressor P53 is a critical regulator of normal cellular homeostasis whose function is either distorted or lost in several cancer types including colorectal cancer. A small group of microRNAs have come to be recognized as essential mediators of P53 function. In a genome-wide systematic approach, we explored miRNAs that are substantially altered by P53 loss and found miR-30e to be the most significantly deregulated miRNA in P53-knockout human colorectal cancer cells. We identified miR-30e-5p to be a novel direct transcriptional target of P53 with gain and loss of function experiments revealing miR-30e-5p to be a significant regulator of tumor cell migration, invasion and in vivo metastasis mediated in part by integrins alpha-6 and beta-1 as novel targets...
June 28, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28656088/effects-of-berberine-on-the-secretion-of-cytokines-and-expression-of-genes-involved-in-cell-cycle-regulation-in-thp-1-monocytic-cell-line
#6
Saeed Mohammadi, Fakhri Sadat Seyedhoseini, Jahanbakhsh Asadi, Yaghoub Yazdani
OBJECTIVES: Current acute myeloid leukemia (AML) therapeutic strategies have irreversible side-effects. Berberine (BBR) is an isoquinoline alkaloid, which has been known as an aryl hydrocarbon receptor (AhR) ligand. AhR is a cytoplasmic receptor, which is involved in the regulation of cellular and immune responses. Here, we investigated the expression profile of genes involved in the cell cycle and different cytokines upon BBR-mediated AhR activation on AML THP-1 cell line. MATERIALS AND METHODS: THP-1 cells and normal monocytes were treated with different concentrations of BBR (10 μM, 25 μM, 50 μM, and 100 μM) for 24 and 48 hr...
May 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28652014/anti-inflammatory-effect-of-man-pen-fang-a-chinese-herbal-compound-on-chronic-pelvic-inflammation-in-rats
#7
Li-Jun Zhang, Jian-Yong Zhu, Meng-Yao Sun, Ya-Nan Song, Khalid Rahman, Cheng Peng, Miao Zhang, Yu-Mei Ye, Hong Zhang
ETHNOPHARMACOLOGICAL RELEVANCE: Traditional Chinese Medicine (TCM) has become the focus of research for the treatment of chronic pelvic inflammatory disease (CPID) based on unique medical theory system. Man-Pen-Fang (MPF), a Chinese herbal compound, which is composed of Thlaspi arvense L. (Cruciferae), Gleditsia sinensis Lam. (Leguminosae), Smilax china L. (Liliaceae), Euonymus alatus (Thunb.) Sieb. (Celastraceae) and Vaccaria segetalis (Neck.) (Caryophyllaceae) MPF has been used for the treatment of CPID and exerted significant clinical curative effects...
June 23, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28631711/-expansion-of-secretory-cells-in-the-fallopian-tubal-epithelium-in-the-early-stages-of-the-pathogenesis-of-ovarian-serous-carcinomas
#8
A V Asaturova, L S Ezhova, N M Faizullina, L V Adamyan, G N Khabas, M V Sannikova
AIM: to investigate the frequency of the types of fallopian tubal secretory cell expansion (SCE) in diseases of the reproductive organs and to determine the immunophenotype and biological role of the cells in the early stages of the pathogenesis of high-grade ovarian serous carcinomas (HGOSC). SUBJECTS AND METHODS: The investigation enrolled 287 patients with extraovarian diseases and ovarian serous tumors varying in grade, whose fallopian tubes were morphologically and immunohistochemically examined using p53, Ki-67, PAX2, Bcl-2, beta-catenin, and ALDH1 markers...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/28584793/clinical-significance-of-p53-protein-expression-beta-catenin-expression-and-her2-expression-for-epstein-barr-virus-associated-gastric-cancer
#9
Dong Won Baek, Byung Woog Kang, Soyoon Hwang, Jong Gwang Kim, An Na Seo, Han Ik Bae, Oh Kyoung Kwon, Seung Soo Lee, Ho Young Chung, Wansik Yu
This study assessed the expression of the p53 protein, beta-catenin, and HER2 and their prognostic implications in patients with EBV-associated gastric cancer (EBVaGC). After reviewing 1318 consecutive cases of surgically resected or endoscopic submucosal dissected gastric cancers, 117 patients were identified as EBV-positive using EBV-encoded RNA in-situ hybridization. The immunohistochemistry results were interpreted as follows: strong p53 nuclear expression in at least 50% of tumor nuclei was interpreted as a positive result, strong beta-catenin expression in at least 10% of cytoplasmic nuclei was interpreted as a positive result, and moderate or strong complete or basolateral membrane staining in 10% of tumor cells was interpreted as a positive result for HER2...
May 2017: Chonnam Medical Journal
https://www.readbyqxmd.com/read/28582348/phenotypic-intratumoral-heterogeneity-of-endometrial-carcinomas
#10
Cátia Silva, Ana S Pires-Luís, Eduardo Rocha, Carla Bartosch, José M Lopes
Intratumoral heterogeneity has been shown to play an important role in diagnostic accuracy, development of treatment resistance, and prognosis of cancer patients. Recent studies have proposed quantitative measurement of phenotypic intratumoral heterogeneity, but no study is yet available in endometrial carcinomas. In our study we evaluated the phenotypic intratumoral heterogeneity of a consecutive series of 10 endometrial carcinomas using measures of dispersion and diversity. Morphometric architectural (%tumor cells, %solid tumor, %differentiated tumor, and %lumens) and nuclear [volume-weighted mean nuclear volume (ν¯v)] parameters, as well as estrogen receptor, progesterone receptor, p53, vimentin, and beta-catenin immunoexpression (H-score) were digitally analyzed in 20 microscopic fields per carcinoma...
June 2, 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28569794/p7c3-inhibits-gsk3%C3%AE-activation-to-protect-dopaminergic-neurons-against-neurotoxin-induced-cell-death-in-vitro-and-in-vivo
#11
Chao Gu, Yan Zhang, Qingsong Hu, Jiayuan Wu, Haigang Ren, Chun-Feng Liu, Guanghui Wang
Parkinson's disease (PD) is the second most prevalent neurodegenerative disease. Although its pathogenesis remains unclear, mitochondrial dysfunction plays a vital role in the pathology of PD. P7C3, an aminopropyl carbazole, possesses a significant neuroprotective ability in several neurodegenerative disorders, including PD. Here, we showed that P7C3 stabilized mitochondrial membrane potential, reduced reactive oxygen species production, and inhibited cytochrome c release in MES23.5 cells (a dopaminergic (DA) cell line) exposed to 1-methyl-4-phenylpyridinium (MPP(+))...
June 1, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28554861/galantamine-alleviates-senescence-of-u87-cells-induced-by-beta-amyloid-through-decreasing-ros-production
#12
Yan Zhang, Lingling Zhao, Zhaoxuan Wu, Xinxin Chen, Tong Ma
Galantamine, which is currently used in the treatment of Alzheimer's disease (AD), has been shown to exert a neuroprotective effect against beta-amyloid (Aβ) peptide-induced toxicity, a critical component involved in the pathogenesis of AD. The aim of this study was to examine the effects of galantamine on proliferation, senescence and ROS production in a U87 cell line treated with Aβ. With the use of a Cell Counting Kit-8 and β galactosidase staining assay, we observed that galantamine (0.3μM) pretreatment significantly prevented Aβ1-40-induced cell degradation and senescence...
July 13, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28550734/pterostilbene-alleviates-polymicrobial-sepsis-induced-liver-injury-possible-role-of-sirt1-signaling
#13
Xiaojing Liu, Xueliang Yang, Lingna Han, Feng Ye, Min Liu, Wanhu Fan, Kai Zhang, Ying Kong, Jian Zhang, Lei Shi, Yunru Chen, Xi Zhang, Shumei Lin
Liver injury occurs frequently during sepsis. Pterostilbene (Pte), a natural dimethylated analog of resveratrol from blueberries, exerts anti-inflammatory and anti-apoptotic effects in various diseases. However, the role of Pte in sepsis-induced liver injury and its underlying mechanisms remain unknown. The current study aimed to evaluate the protective effects of Pte on sepsis-induced liver injury and its potential mechanisms. Sepsis was induced using cecal ligation and puncture (CLP) in C57BL/6 mice. Mice were administered Pte (5, 10, 15mg/kg, i...
May 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28525372/overexpression-of-a-novel-candidate-oncogene-kif14-correlates-with-tumor-progression-and-poor-prognosis-in-prostate-cancer
#14
Yixiang Zhang, Yeqing Yuan, Pei Liang, Zhaoxia Zhang, Xiaojing Guo, Ligang Xia, Yingying Zhao, Xing-Sheng Shu, Shengkun Sun, Ying Ying, Yingduan Cheng
Prostate cancer (PCa) is the second leading cause of death from cancer in men. The mechanism underlying tumorigenesis and development of PCa is largely unknown. Here, we identified Kinesin family member 14 (KIF14) as a novel candidate oncogene in PCa. We found that KIF14 was overexpressed in multiple PCa cell lines and primary PCa tissues. Knockdown of KIF14 in DU145 and PC3 prostate cancer cells suppressed cell proliferation, induced cell cycle arrest and apoptosis. Transcriptome analysis by RNA-sequencing demonstrated that KIF4 suppression led to transcriptional changes of genes involved in p53 and TGF-beta signaling pathway...
July 11, 2017: Oncotarget
https://www.readbyqxmd.com/read/28486965/site-specific-gene-expression-patterns-in-oral-cancer
#15
Gesche Frohwitter, Horst Buerger, Eberhard Korsching, Paul J van Diest, Johannes Kleinheinz, Thomas Fillies
BACKGROUND: Squamous cell carcinomas (SCCs) are the most prevalent malignant tumours within the head and neck. Evidence exists that distinct genes are differentially regulated in SCCs of the oral cavity compared to other head and neck regions. Given this background, the aim of this study was to investigate whether such tumour site-specific gene expression can also be observed in different localizations within the oral cavity. METHODS: Using tissue microarrays (TMAs), we investigated 76 SCCs of the floor of the mouth, 49 SCCs of the tongue and 68 SCCs of other anatomic regions within the oral cavity...
May 10, 2017: Head & Face Medicine
https://www.readbyqxmd.com/read/28471483/human-sle-bone-marrow-mesenchymal-stem-cells-bmscs-have-a-senescence-associated-secretory-phenotype-sasp-mediated-by-a-mavs-ifn%C3%AE-feedback-loop
#16
Lin Gao, Anna K Bird, Nida Meednu, Kristin Dauenhauer, Jane Liesveld, Jennifer Anolik, R John Looney
Objective - BMSCs create a special microenvironment for hematopoiesis and immunity and also display robust immunomodulatory properties which are impaired in SLE. This study was undertaken to define the mechanisms of defects in human SLE BMSCs. Methods - Patients fulfilling SLE classification criteria and healthy controls were recruited under an Institutional Review Board approved protocol (n=6 each). BMSCs were isolated with low density Ficoll/Hypaque. BMSCs were verified by flow cytometry and studied using immunocytochemistry, real-time PCR, western blotting, comet assay, beta-galactosidase assay, and RNA interference...
May 4, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28431977/transcriptome-comparison-reveals-insights-into-muscle-response-to-hypoxia-in-blunt-snout-bream-megalobrama-amblycephala
#17
Bo-Xiang Chen, Shao-Kui Yi, Wei-Feng Wang, Yan He, Yan Huang, Ze-Xia Gao, Hong Liu, Wei-Min Wang, Huan-Ling Wang
The economic and biological significance of blunt snout bream (Megalobrama amblycephala) makes this species important to explore the underlying molecular mechanism of hypoxia response. In the present study, we compared the transcriptional responses to serious hypoxia in skeletal muscle among hypoxia tolerant (MT), sensitive (MS) and control (without hypoxia treatment, MC) M. amblycephala obtained according to the time difference of losing balance after hypoxia treatment. A total of 88,200,889 clean reads were generated and assembled into 44,493 unigenes...
August 15, 2017: Gene
https://www.readbyqxmd.com/read/28423568/sepia-ink-oligopeptide-induces-apoptosis-and-growth-inhibition-in-human-lung-cancer-cells
#18
Zhi Zhang, Lei Sun, Guoren Zhou, Peng Xie, Jinjun Ye
Sepia ink oligopeptide (SIO), as a tripeptide extracted from Sepia ink, could be used as an inducer of apoptosis in human prostate cancer cells. We designed a cyclo-mimetic peptide of SIO by introducing a disulfide bond to stabilize the native peptide into beta turn structure, and produced a peptide with higher cell permeability and stability. Through labeling an FITC to the N-terminus of the peptide, the cell permeability was examined. Stabilized peptide showed enhanced cellular uptake than linear tripeptide as indicated by flow cytometry and cell fluorescent imaging...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28420489/mast-cells-emerge-as-mediators-of-atherosclerosis-special-emphasis-on-il-37-inhibition
#19
REVIEW
Pio Conti, Gianfranco Lessiani, Spyridon K Kritas, Gianpaolo Ronconi, Aessandro Caraffa, Theoharis C Theoharides
In atherosclerosis lipoproteins stimulate the innate immune response, leading to the release of inflammatory cytokines and chemokines. Hypercholesterolemia may activate the synthesis and release of inflammatory cytokines such as IL-1, which induces TNF release in mast cells (MCs). IL-1 and IL-1 family members orchestrate a broadening list of inflammatory diseases, including atherosclerosis. MCs are implicated in the pathophysiology of several diseases including allergy and inflammation. Activated MCs, located perivascularly, contribute to inflammation in atherosclerosis by producing inflammatory cytokines...
June 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28388558/expression-of-estrogen-receptors-and-androgen-receptor-and-their-clinical-significance-in-gastric-cancer
#20
Wenbo Tang, Rujiao Liu, Yan Yan, Xiaoli Pan, Minjun Wang, Xiaotian Han, Hui Ren, Zhe Zhang
Despite the mounting studies exploring the role of estrogen receptor alpha (ERα), estrogen receptor beta (ERβ) and androgen receptor (AR) in gastric cancer (GC), there remain controversies in those findings. The present study investigated the expression of ERα, ERβ and AR in Chinese gastric cancer by immunohistochemistry, analyzed their clinical relevance in gastric cancer, and examined the potential mechanisms by which ERα and AR modulated GC progression. The positive rate of ERα, ERβ and AR in GC tissues was 6% (9/150), 93...
June 20, 2017: Oncotarget
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