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https://www.readbyqxmd.com/read/29656213/molecular-characterization-of-voltage-gated-potassium-channel-kv-and-its-importance-in-functional-dynamics-in-bull-spermatozoa
#1
Rishi Kumar Gupta, Dilip Kumar Swain, Vijay Singh, Mukul Anand, Soumen Choudhury, Sarvajeet Yadav, Atul Saxena, Satish Kumar Garg
Present study was undertaken to characterize the voltage gated potassium channel (Kv 1.1) in bull spermatozoa using sixty four ejaculates collected from four Hariana bulls. Functional characterization was undertaken using a selective blocker of Kv channel, 4-Aminopyridine (4-AP) while molecular presence of Kv on bull spermatozoa by immunoblotting and indirect immunofluorescence. Three sets of 100 μL diluted sperm samples namely-negative control (100 μL of sperm dilution medium (SDM) containing 10 × 106  cells), vehicle control (99 μL of SDM containing 10 × 106  cells, and DMSO- 1  μL) and 4-AP treatment group (99 μL of SDM containing 10 × 106  cells, and drug 1 μL 4-AP) were used in the study...
March 26, 2018: Theriogenology
https://www.readbyqxmd.com/read/29649580/differential-responses-of-autonomic-function-in-sea-level-residents-acclimatized-lowlanders-at-3500-m-and-himalayan-high-altitude-natives-at-3500-m-a-cross-sectional-study
#2
Priyanka Dhar, Vijay K Sharma, Saroj K Das, Kalpana Barhwal, Sunil K Hota, Shashi B Singh
We studied the differential responses of autonomic function in sea level residents (SLR), acclimatized lowlanders (ALH) in high altitude (HA) and HA natives (HAN) at >3500 m. Out of 771 male volunteers included in this cross-sectional study, SLR, ALH and HAN groups were comprised of 351, 307 and 113 volunteers, respectively. Our results showed persistent sympathetic dominance with significantly reduced (p < 0.05) parasympathetic response in ALH as compared to SLR and HAN populations. This may be attributed to significantly increased (p < 0...
April 9, 2018: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/29624023/effect-of-nitric-oxide-on-boar-sperm-motility-membrane-integrity-and-acrosomal-status-during-semen-storage
#3
M Jovicić, E Pintus, T Fenclova, O Simonik, E Chmelikova, J L Ros-Santaella, M Sedmikova
Nitric oxide (NO) is a major gasotransmitter involved in several physiological processes of male reproduction. There is, nevertheless, little information concerning the role of NO during semen storage. The aim of this study was to evaluate the effect of NO on boar semen stored at 17oC for 72 h. For this purporse, sperm samples were treated with 0.625, 1.25, 2.5, 5, and 10 mM aminoguanidine (AG) or Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), a selective and non-selective NO synthase (NOS) inhibitor, respectively...
March 2018: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29580782/physiological-differences-between-female-limited-alternative-life-history-strategies-the-alba-phenotype-in-the-butterfly-colias-croceus
#4
Alyssa Woronik, Constanti Stefanescu, Reijo Käkelä, Christopher W Wheat, Philipp Lehmann
Across a wide range of taxa, individuals within populations exhibit alternative life history strategies (ALHS) where their phenotypes dramatically differ due to divergent investments in growth, reproduction and survivorship, with the resulting trade-offs directly impacting Darwinian fitness. Though the maintenance of ALHS within populations is fairly well understood, little is known regarding the physiological mechanisms that underlie ALHS and how environmental conditions can affect the evolution and expression of these phenotypes...
March 23, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29580753/sephadex-filtration-as-successful-alternative-to-density-gradient-centrifugation-procedures-for-ram-sperm-selection-with-improved-kinetics
#5
D A Galarza, A López-Sebastián, H Woelders, E Blesbois, J Santiago-Moreno
Density-gradients centrifugation (DGC) and filtration columns (FC) are used to separate deformed or dead sperm, debris, and other cells that may negatively affect the fertilizing capacity of sperm in fresh, chilled and frozen/thawed semen. The present study was conducted to evaluate the suitability of DGC (BoviPure®, Percoll® and Accudenz®) and FC (Sephadex G-15®) sperm selection procedures for fresh-extended and cold-stored ram semen by assessment of post-treatment sperm quality variables. Twenty normospermic ejaculates from ten adult Merino rams were used...
March 20, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29572753/how-do-we-approach-benign-proliferative-lesions
#6
REVIEW
Faina Nakhlis
PURPOSE OF REVIEW: The aim of this review is to summarize recently published literature addressing atypical ductal hyperplasia (ADH), lobular neoplasia (atypical lobular hyperplasia [ALH] and classic lobular carcinoma in situ [C-LCIS]), non-classic lobular carcinoma in situ (NC-LCIS), papillary lesions, and flat epithelial atypia (FEA). RECENT FINDINGS: While ADH, ALN, and C-LCIS are well-established markers of an increased risk of future breast cancers, the risk implications are less clear for papillary lesions and FEA...
March 23, 2018: Current Oncology Reports
https://www.readbyqxmd.com/read/29565068/on-the-higher-order-t-2-%C3%A2-e-t-2-jahn-teller-coupling-effects-in-the-photodetachment-spectrum-of-the-alanate-anion-alh-4
#7
T Mondal
The static and dynamic aspects of the T2 ⊗ (e + t2) Jahn-Teller coupling effects in the X[combining tilde]2T2 electronic state of the AlH4 radical are investigated theoretically by probing the first photodetachment spectrum of AlH4-. Associated potential energy surfaces up to two-dimensional space of nuclear coordinates are established from extensive electronic structure calculations and nuclear dynamics calculations are done on them via wave packet propagation including the nonadiabatic coupling of the three electronic sheets...
March 22, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29527734/fosb-immunoreactivity-in-endothelia-of-epithelioid-hemangioma-angiolymphoid-hyperplasia-with-eosinophilia
#8
Ana Ortins-Pina, Mar Llamas-Velasco, Sara Turpin, Luís Soares-de-Almeida, Paulo Filipe, Heinz Kutzner
BACKGROUND: Accurate distinction of epithelioid hemangioma (EH) from its malignant mimics is paramount but it remains challenging due to its wide morphological spectrum and lack of objective molecular markers. FOSB oncogenic activation was recently identified as a key event in endothelial proliferation. We sought to investigate the FOSB staining pattern in EH with angiolymphoid hyperplasia with eosinophilia morphology (EH-AHLE) and to evaluate its value in differential diagnosis of epithelioid vascular tumors...
March 12, 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29521921/treatment-of-dehisced-thoracic-neonatal-wounds-with-single-use-negative-pressure-wound-therapy-device-and-medical-grade-honey-a-retrospective-case-series
#9
Vita Boyar
PURPOSE: The purpose of this study was to report on our experience with a portable, single-use negative pressure wound therapy device used in combination with activated active Leptospermum honey (ALH) in the treatment of colonized or infected, dehisced, thoracic wounds in neonates with complex congenital heart disease. DESIGN: Retrospective, descriptive study. SUBJECTS AND SETTING: We reviewed medical records of 18 neonates and reported on findings from 11; the remaining 7 were not included secondary to incomplete records, transfer to a different institution prior to wound healing, or death...
March 2018: Journal of Wound, Ostomy, and Continence Nursing
https://www.readbyqxmd.com/read/29513298/nod2-activation-during-bacterial-and-viral-infections-polymorphisms-and-potential-as-therapeutic-target
#10
Diana Alhelí Domínguez-Martínez, Daniel Núñez-Avellaneda, Carlos Alberto Castañón-Sánchez, Ma Isabel Salazar
Nucleotide-binding domain (NBD) leucine-rich repeat (LRR)-containing receptors or NLRs are a family of receptors that detect both, molecules associated to pathogens and alarmins, and are located mainly in the cytoplasm. NOD2 belongs to the NLR family and is a dynamic receptor capable of interacting with multiple proteins and modulate immune responses in a stimuli-dependent manner. The experimental evidence shows that interaction between NOD2 structural domains and the effector proteins shape the overall response against bacterial or viral infections...
2018: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
https://www.readbyqxmd.com/read/29445571/immunoreactivity-of-wilms-tumor-1-wt1-as-an-additional-evidence-supporting-hemangiomatous-rather-than-inflammatory-origin-in-the-etiopathogenesis-of-angiolymphoid-hyperplasia-with-eosinophilia
#11
Fatma Tokat, Julia S Lehman, Engin Sezer, Emel Dikicioglu Cetin, Umit Ince, Emel Ozturk Durmaz
Background: Angiolymphoid hyperplasia with eosinophilia (ALHE) is a rare vascular proliferative disorder mainly located in the periauricular region. The etiopathogenesis of ALHE is unknown, and it is still controversial as to whether the entity represents a benign vascular neoplasm or an inflammatory process. Aim: Recently, the intracytoplasmic staining pattern of Wilms tumor 1 (WT1) on immunohistochemistry has highlighted true vascular neoplasms, such as microvenular hemangioma, tufted angioma, and spindle cell hemangioma, which has made it helpful to distinguish ALHE from vascular malformations, as there is a negative staining pattern in the other entities...
January 2018: Dermatology Practical & Conceptual
https://www.readbyqxmd.com/read/29383768/effects-of-the-supplementation-with-an-high-polyphenols-extra-virgin-olive-oil-on-kinetic-sperm-features-and-seminal-plasma-oxidative-status-in-healthy-dogs
#12
V Tufarelli, A Rizzo, G M Lacalandra, A C Guaricci, V Laudadio, L Valentini
The aim of this work was to evaluate the effects of the supplementation of two extra-virgin olive oils (EVOO) having different polyphenols content, on canine spermatozoa kinetic parameters and seminal plasma oxidative status. The study was conducted on 12 clinically healthy dogs of different breeds (2-7 years, 5-48 kg of body weight) divided into two groups: an experimental group supplemented with EVOO (Coratina cultivar) high in polyphenols (H-P) and a control group fed EVOO (Cima di Bitonto cultivar) low in polyphenols (L-P)...
January 30, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29369048/reaction-paths-of-alane-dissociation-on-the-si-001-surface
#13
Richard Smith, David R Bowler
Building on our earlier study, we examine the kinetic barriers to decomposition of alane, AlH3, on the Si(001) surface, using the nudged elastic band (NEB) approach within DFT. We find that the initial decomposition to AlH with two H atoms on the surface proceeds without a significant barrier. There are several pathways available to lose the final hydrogen, though these present barriers of up to 1 eV. Incorporation is more challenging, with the initial structures less stable in several cases than the starting structures, just as was found for phosphorus...
January 25, 2018: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29325246/-diagnostic-significance-of-lymph-node-core-needle-biopsy-for-lymphoproliferative-disease-a-clinicopathologic-study-of-1-013-cases
#14
R F Huang, W Y Zhang, W P Liu, S Zhao, Y X Ye, H Sun, L M Gao, J C Wang, Q P Yang
Objective: To study the clinicopathologic features of lymphoproliferative disease by lymph node core needle biopsy(CNB)and to evaluate the diagnostic significance of CNB for lymphoproliferative disease. Methods: The annual distribution, entity constitute, clinical finding, gross feature, morphologic change, affiliate study and repeat biopsy diagnosis of 1 013 cases of lymph node CNB diagnosed at West China Hospital of Sichuan University from January 2009 to December 2015 were investigated. Results: (1) Proportion of lymph node CNB in total amount of biopsy specimens increased from 0...
January 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/29301674/influence-of-cooling-temperature-in-sperm-subpopulations-of-domestic-cats
#15
REVIEW
Anne Kemmer Souza, Luiz Guilherme Corsi Trautwein, Cristiane Sella Paranzini, Felipe Montanheiro Perencin, Guilherme Schiess Cardoso, Maria Isabel Mello Martins
The objective of this study was to identify and compare domestic feline sperm subpopulations, chilled at -1 °C for 24 and 48 h, as well as to analyze the sperm frequency in different subpopulations. Ten adult cats were used. Sperm collection was performed using electroejaculation (EEJ). Spermatic kinetics were evaluated using a computerized system at three moments: fresh, 24 and 48 h after refrigeration. The ejaculates were divided into a group refrigerated at -1 °C (n = 5,) and a group refrigerated at 4 °C (n = 5),...
February 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29299726/observation-versus-excision-of-lobular-neoplasia-on-core-needle-biopsy-of-the-breast
#16
Hank Schmidt, Brittany Arditi, Margaux Wooster, Christina Weltz, Laurie Margolies, Ira Bleiweiss, Elisa Port, Shabnam Jaffer
PURPOSE: Controversy surrounds management of lobular neoplasia (LN), [atypical lobular hyperplasia (ALH) or lobular carcinoma in situ (LCIS)], diagnosed on core needle biopsy (CNB). Retrospective series of pure ALH and LCIS reported "upgrade" rate to DCIS or invasive cancer in 0-40%. Few reports document radiologic/pathologic correlation to exclude cases of discordance that are the likely source of most upgrades, and there is minimal data on outcomes with follow-up imaging and clinical surveillance...
April 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29290453/influence-of-feeding-supplements-of-almond-hulls-and-ensiled-citrus-pulp-on-the-milk-production-milk-composition-and-methane-emissions-of-dairy-cows
#17
S R O Williams, A V Chaves, M H Deighton, J L Jacobs, M C Hannah, B E Ribaux, G L Morris, W J Wales, P J Moate
Almond hulls and citrus pulp have been fed to dairy cows with variable responses for milk production, but no information exists on their effect on enteric methane emissions. This experiment examined the effects of dietary supplementation with either almond hulls or ensiled citrus pulp on the milk yield, milk composition, and enteric methane emissions of dairy cows. Thirty-two Holstein dairy cows in mid lactation were offered 1 of 3 diets over a 28-d experiment. Twelve cows received a control (CON) diet, 10 cows a diet containing almond hulls (ALH), and 10 cows a diet containing ensiled citrus pulp (CIT)...
March 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29258587/association-between-mammographic-breast-density-and-histologic-features-of-benign-breast-disease
#18
Karthik Ghosh, Robert A Vierkant, Ryan D Frank, Stacey Winham, Daniel W Visscher, Vernon S Pankratz, Christopher G Scott, Kathleen Brandt, Mark E Sherman, Derek C Radisky, Marlene H Frost, Lynn C Hartmann, Amy C Degnim, Celine M Vachon
BACKGROUND: Over 40% of women undergoing breast screening have mammographically dense breasts. Elevated mammographic breast density (MBD) is an established breast cancer risk factor and is known to mask tumors within the dense tissue. However, the association of MBD with high risk benign breast disease (BBD) is unknown. METHOD: We analyzed data for 3400 women diagnosed with pathologically confirmed BBD in the Mayo Clinic BBD cohort from 1985-2001, with a clinical MBD measure (either parenchymal pattern (PP) or Breast Imaging Reporting and Data Systems (BI-RADS) density) and expert pathology review...
December 19, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29181182/solitary-nodule-of-angiolymphoid-hyperplasia-with-eosinophilia-of-the-back-masquerading-as-pyogenic-granuloma
#19
Shu-Lin Li, Jian-De Han
Angiolymphoid hyperplasia with eosinophilia (ALHE) is a rare chronic inflammatory disorder of unknown etiology that most commonly presents as painless lymphadenopathy or subcutaneous masses in the head and neck region. The pathogenesis of the disease is not yet clear. The differential diagnosis is determined using characteristic histological features, such as significant vascular proliferation, lymphocytes and eosinophil inflammation in the dermis. Here is presented the case of a patient with a solitary nodule of ALHE masquerading as pyogenic granuloma (PG)...
November 2017: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29179901/ct-imaging-of-complications-associated-with-continuous-flow-left-ventricular-assist-devices-lvads
#20
Girish S Shroff, Daniel Ocazionez, Bindu Akkanti, Daniel Vargas, Alheli Garza, Pushpender Gupta, Jayeshkumar A Patel, Manish K Patel, Igor D Gregoric
Heart failure is becoming increasingly prevalent, and more patients are being treated with left ventricular assist devices (LVADs), either as a bridge to transplant or as destination therapy. The use of continuous-flow LVADs is on the rise. LVAD therapy is associated with several classes of complications, including bleeding, thrombosis, and infection. CT imaging can be used effectively to diagnose LVAD complications, including mediastinal hematomas and pericardial, abdominal wall, and retroperitoneal hemorrhage, inflow and outflow graft and aortic thrombi, and driveline and pump pocket infections...
December 2017: Seminars in Ultrasound, CT, and MR
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