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https://www.readbyqxmd.com/read/27885466/molecular-epidemiology-and-multilocus-sequence-analysis-of-potentially-zoonotic-giardia-spp-from-humans-and-dogs-in-jamaica
#1
Mellesia F Lee, Paul Cadogan, Sarah Eytle, Sonia Copeland, Julia Walochnik, John F Lindo
Giardia spp. are the causative agents of intestinal infections in a wide variety of mammals including humans and companion animals. Dogs may be reservoirs of zoonotic Giardia spp.; however, the potential for transmission between dogs and humans in Jamaica has not been studied. Conventional PCR was used to screen 285 human and 225 dog stool samples for Giardia targeting the SSU rDNA gene followed by multilocus sequencing of the triosephosphate isomerase (tpi), glutamate dehydrogenase (gdh), and β-giardin (bg) genes...
November 25, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27885465/multilocus-sequence-analysis-of-giardia-spp-isolated-from-patients-with-diarrhea-in-austria
#2
Mellesia F Lee, Herbert Auer, John F Lindo, Julia Walochnik
Giardia duodenalis is a protozoan parasite causing intestinal infections in a wide range of mammals. Two distinct assemblages, A and B, infect humans predominantly; however, both are believed to be generally zoonotic. Giardia strains associated with infections in Austria have not been investigated at the molecular level. In this study, 65 human stool samples microscopically positive for Giardia spp. were subjected to DNA isolation and nested PCR targeting fragments of the glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), and beta-gardin (bg) genes...
November 25, 2016: Parasitology Research
https://www.readbyqxmd.com/read/27877179/genome-wide-identification-of-micrornas-and-their-targets-in-the-leaves-and-fruits-of-eucommia-ulmoides-using-high-throughput-sequencing
#3
Lin Wang, Hongyan Du, Ta-Na Wuyun
MicroRNAs (miRNAs), a group of endogenous small non-coding RNAs, play important roles in plant growth, development, and stress response processes. Eucommia ulmoides Oliver (hardy rubber tree) is one of the few woody plants capable of producing trans-1, 4-polyisoprene (TPI), also known as Eu-rubber, which has been utilized as an industrial raw material and is extensively cultivated in China. However, the mechanism of TPI biosynthesis has not been identified in E. ulmoides. To characterize small RNAs and their targets with potential biological roles involved in the TPI biosynthesis in E...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27876969/molecular-detection-of-giardiasis-among-children-at-cairo-university-pediatrics-hospitals
#4
Marwa A Ghieth, Magd A Kotb, Enas Y Abu-Sarea, Ayman A El-Badry
Giardiasis is a prevailing intestinal disease in children. This study aimed to determine molecular prevalence of Giardia intestinalis in children attending Cairo University Pediatrics Hospitals, using copro-PCR assays, conventional methods and to evaluate diagnostic effectiveness of used tests. 229 fecal samples were collected from children suffering from gastrointestinal symptoms and examined for Giardia by microscopy, Immuno-chromatographic test (ICT), copro-DNA using two PCR assays targeting tpi [nested-PCR (nPCR)] and 18S [conventional-PCR (cPCR)] genes...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27852150/frequency-diagnostic-performance-of-coproantigen-detection-and-genotyping-of-the-giardia-among-patients-referred-to-a-multi-level-teaching-hospital-in-northern-india
#5
Ujjala Ghoshal, Ratnakar Shukla, Priyannk Pant, Uday C Ghoshal
Giardiasis, a common gastrointestinal parasitic infection in tropics, is diagnosed on stool microscopy (gold standard); however, its sensitivity is low due to intermittent fecal shedding. Coproantigen detection (ELISA) is useful but requires further evaluation. We aimed to study: (a) detection of Giardia by stool microscopy and/or coproantigen, (b) diagnostic performance of fecal antigen detection and microscopy, and c) genotypic characterization of G. lamblia using PCR specific for triose phosphate isomerase (tpi) gene...
November 17, 2016: Pathogens and Global Health
https://www.readbyqxmd.com/read/27846985/array-of-biosensors-for-discrimination-of-grapes-according-to-grape-variety-vintage-and-ripeness
#6
C Medina-Plaza, J A de Saja, J A Fernández-Escudero, E Barajas, G Medrano, M L Rodriguez-Mendez
A bioelectronic tongue based on nanostructured biosensors specific for the simultaneous detection of sugars and phenols has been developed. The array combined oxidases and dehydrogenases immobilized on a lipidic layer prepared using the Langmuir-Blodgett technique where Glucose oxidase, d-Fructose dehydrogenase, Tyrosinase or Laccase were imbibed. A phthalocyanine was co-immobilized in the sensing layer and used as electron mediator. The array thus formed has been used to analyze grapes and provides global information about the samples while providing specific information about their phenolic and their sugar content...
December 1, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27843774/long-term-clinical-outcome-of-peripheral-nerve-stimulation-for-chronic-headache-and-complication-prevention
#7
Linqiu Zhou, Avi Ashkenazi, Joseph W Smith, Na Jen, Timothy R Deer, Chen Zhou
BACKGROUND: Subcutaneous peripheral nerve stimulation (PNS) has emerged as a useful tool in the treatment of intractable headaches. However, complications such as skin erosion, infection and lead migration have adversely affected clinical outcome, and occasionally led to treatment cessation. OBJECTIVES: Here we report the results of peripheral nerve stimulator implantation performed on 24 patients with various chronic headaches at our center over a period of 9 years...
August 2016: Anesthesiology and Pain Medicine
https://www.readbyqxmd.com/read/27832466/cost-effectiveness-analysis-of-the-temporary-percutaneous-ileostomy-for-faecal-diversion-after-colorectal-resection-in-elderly
#8
W Bugiantella, F Rondelli, L Mariani, A Polistena, A Sanguinetti, N Avenia, E Mariani
BACKGROUND: Conventional loop ileostomy (CLI) is a suitable procedure for transitory faecal diversion after colorectal anastomosis, but it causes relevant morbidities (dehydration, discomfort, peristomal infections) and requires a second operation to be closed. We already described an alternative technique of temporary percutaneous ileostomy (TPI), which can be removed without surgery. AIMS: We analyse the outcomes and the costs of the TPI in protecting low colorectal anastomosis in elderly, compared to the CLI...
November 10, 2016: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/27828417/two-stage-optical-recording-photoinduced-birefringence-and-surface-mediated-bits-storage-in-bisazo-containing-copolymers-towards-ultrahigh-data-memory
#9
Yanlei Hu, Dong Wu, Jiawen Li, Wenhao Huang, Jiaru Chu
Ultrahigh density data storage is in high demand in the current age of big data and thus motivates many innovative storage technologies. Femtosecond laser induced multi-dimensional optical data storage is an appealing method to fulfill the demand of ultrahigh storage capacity. Here we report a femtosecond laser induced two-stage optical storage in bisazobenzene copolymer films by manipulating the recording energies. Different mechanisms can be selected for specified memory use: two-photon isomerization (TPI) and laser induced surface deformation...
October 3, 2016: Optics Express
https://www.readbyqxmd.com/read/27760188/genotyping-and-descriptive-proteomics-of-a-potential-zoonotic-canine-strain-of-giardia-duodenalis-infective-to-mice
#10
Camila Henriques Coelho, Adriana Oliveira Costa, Ana Carolina Carvalho Silva, Maíra Mazzoni Pucci, Angela Vieira Serufo, Haendel Goncalves Nogueira Oliveira Busatti, Maurício Durigan, Jonas Perales, Alex Chapeaurouge, Daniel Almeida da Silva E Silva, Maria Aparecida Gomes, Juliano Simões Toledo, Steven M Singer, Rosiane A Silva-Pereira, Ana Paula Fernandes
The zoonotic potential of giardiasis, as proposed by WHO since the late 70's, has been largely confirmed in this century. The genetic assemblages A and B of Giardia duodenalis are frequently isolated from human and canine hosts. Most of the assemblage A strains are not infective to adult mice, which can limit the range of studies regarding to biology of G. duodenalis, including virulence factors and the interaction with host immune system. This study aimed to determine the infectivity in mice of an assemblage A Giardia duodenalis strain (BHFC1) isolated from a dog and to classify the strain in sub-assemblages (AI, AII, AIII) through the phylogenetic analysis of beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh) genes...
2016: PloS One
https://www.readbyqxmd.com/read/27737706/prevalence-and-multilocus-genotyping-of-giardia-duodenalis-in-dairy-calves-in-xinjiang-northwestern-china
#11
Meng Qi, Haiyan Wang, Bo Jing, Rongjun Wang, Fuchun Jian, Changshen Ning, Longxian Zhang
BACKGROUND: Giardia duodenalis is an important protozoan parasite. It is an established zoonotic pathogen and dairy calves have been implicated as one of the most important sources of human infection. This study was conducted to assess the prevalence and multilocus genotyping of G. duodenalis in dairy calves in the Xinjiang Uyghur Autonomous Region, northwestern China. FINDINGS: A total of 514 fresh fecal samples were randomly collected from dairy calves in 15 farms in Xinjiang, 13...
October 13, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27721038/saw-marks-in-bones-a-study-of-170-experimental-false-start-lesions
#12
Luísa Nogueira, Gérald Quatrehomme, Christophe Rallon, Pascal Adalian, Véronique Alunni
This experimental study examined false starts because these are seldom discussed in the forensic literature and usually concern animal bones rather than human bones. We created 170 experimental false starts (50 on pig femurs and 120 on human femurs) using five different saws (4 with an alternating set of teeth, and one with a wavy set). Teeth per inch (TPI) ranged from 7 to 24. Saws were classified as either rip saws or universal saws. The bone lesions were studied using stereomicroscopy. This study focused on three features that are easily observed with a stereomicroscope: the minimum width of the kerf, the profile of the lesions (concave or convex), and the shape of the edges (narrow-wide pattern, necking in the middle, or straight pattern)...
September 28, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27706146/characterisation-of-reproduction-associated-genes-and-peptides-in-the-pest-land-snail-theba-pisana
#13
Michael J Stewart, Tianfang Wang, Bradley I Harding, U Bose, Russell C Wyeth, Kenneth B Storey, Scott F Cummins
Increased understanding of the molecular components involved in reproduction may assist in understanding the evolutionary adaptations used by animals, including hermaphrodites, to produce offspring and retain a continuation of their lineage. In this study, we focus on the Mediterranean snail, Theba pisana, a hermaphroditic land snail that has become a highly invasive pest species within agricultural areas throughout the world. Our analysis of T. pisana CNS tissue has revealed gene transcripts encoding molluscan reproduction-associated proteins including APGWamide, gonadotropin-releasing hormone (GnRH) and an egg-laying hormone (ELH)...
2016: PloS One
https://www.readbyqxmd.com/read/27667393/a-real-time-study-of-the-interaction-of-tbp-with-a-tata-box-containing-duplex-identical-to-an-ancestral-or-minor-allele-of-human-gene-lep-or-tpi
#14
Olga Arkova, Nikita Kuznetsov, Olga Fedorova, Ludmila Savinkova
It is known that only a single-nucleotide substitution (SNP: a single nucleotide polymorphism) in the sequence of a TATA box can influence the affinity of the interaction of TBP with the TATA box and contribute to the pathogenesis of complex hereditary human diseases and sometimes may be a cause of monogenic diseases (for instance, β-thalassemia).. In the present work, we studied the interaction of human TBP with a double-stranded oligodeoxyribonucleotide (ODN) 15 or 26 bp long identical to a TATA box of promoters of a real-life human gene, TPI or LEP, and labeled with fluorophores TAMRA and FAM...
September 25, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27664622/protein-markers-for-discrimination-of-meat-species-in-raw-beef-pork-and-poultry-and-their-mixtures
#15
Gap-Don Kim, Jin-Kyu Seo, Hyeon-Woong Yum, Jin-Yeon Jeong, Han-Sul Yang
The purpose of this study was to find discrimination markers for four major meat species such as beef, pork, chicken and duck. Myofibrillar and sarcoplasmic proteins isolated from each meat type were analyzed by one-dimensional gel electrophoresis and some proteins were identified through LC-MS/MS analysis. We confirmed that troponin I (TnI), enolase 3, l-lactate dehydrogenase (LDH) and triose-phosphate isomerase (TPI) could be useful markers for discrimination of mammals from poultry due to their different electrophoretic mobility...
February 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27647935/the-drug-ornidazole-inhibits-photosynthesis-in-a-different-mechanism-described-for-protozoa-and-anaerobic-bacteria
#16
Yehouda Marcus, Noam Tal, Mordechai Ronen, Raanan Carmieli, Michael Gurevitz
Ornidazole of the 5-nitroimidazole drug family is used to treat protozoan and anaerobic bacterial infections via a mechanism that involves pre-activation by reduction of the nitro group, and production of toxic derivatives and radicals. Metronidazole, another drug family member, has been suggested to affect photosynthesis by draining electrons from the electron carrier, ferredoxin, thus inhibiting NADP(+) reduction and stimulating radical and peroxide production. Here we show, however, that ornidazole inhibits photosynthesis via a different mechanism...
September 19, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27588266/correlation-between-visual-function-and-refractive-topographic-pachymetric-and-aberrometric-data-in-eyes-with-keratoconus
#17
Neslihan Bayraktar Bilen, Ibrahim F Hepsen, Carlos G Arce
AIM: To analyze the relationship between two visual functions and refractive, topographic, pachymetric and aberrometric indicators in eyes with keratoconus. METHODS: Corrected distance visual acuity (CDVA), and letter contrast sensitivity (CS) were correlated with refraction, corneal topography, pachymetry, and total corneal wavefront data prospectively in 71 eyes with keratoconus. The topographic indices assessed were simulated keratometry for the flattest and steepest meridians (SimK1 and SimK2), posterior steeper K (Ks), elevation value in best-fit sphere (BFS) maps, squared eccentricity (Є(2)), aspheric asymmetric index (AAI), pachymetry, thickness progression index (TPI), the amount of pachymetric decentralization (APD), and GalileiTM-keratoconus indices...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27576343/occurrence-and-multilocus-genotyping-of-giardia-intestinalis-assemblage-c-and-d-in-farmed-raccoon-dogs-nyctereutes-procyonoides-in-china
#18
Xiao-Xuan Zhang, Wen-Bin Zheng, Jian-Gang Ma, Qiu-Xia Yao, Yang Zou, Cai-Jia Bubu, Quan Zhao, Xing-Quan Zhu
BACKGROUND: Giardia intestinalis, the only causative agent of human giardiasis, can infect a wide range of animals. As no information concerning the prevalence and genotyping of G. intestinalis in raccoon dogs in China is available, examination of 305 faecal samples from raccoon dogs in Jilin Province (n = 110), Heilongjiang Province (n = 40), Liaoning Province (n = 72), Hebei Province (n = 54) and Shandong Province (n = 29) was conducted to estimate the prevalence of G...
2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27559985/smart-adsorbents-with-photoregulated-molecular-gates-for-both-selective-adsorption-and-efficient-regeneration
#19
Lei Cheng, Yao Jiang, Ni Yan, Shu-Feng Shan, Xiao-Qin Liu, Lin-Bing Sun
Selective adsorption and efficient regeneration are two crucial issues for adsorption processes; unfortunately, only one of them instead of both is favored by traditional adsorbents with fixed pore orifices. Herein, we fabricated a new generation of smart adsorbents through grafting photoresponsive molecules, namely, 4-(3-triethoxysilylpropyl-ureido)azobenzene (AB-TPI), onto pore orifices of the support mesoporous silica. The azobenzene (AB) derivatives serve as the molecular gates of mesopores and are reversibly opened and closed upon light irradiation...
September 7, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27551148/combining-tpi-and-co1-genetic-markers-to-discriminate-invasive-helicoverpa-armigera-from-local-helicoverpa-zea-lepidoptera-noctuidae-populations-in-the-southeastern-united-states
#20
Rodney N Nagoshi, Todd M Gilligan, Julieta Brambila
The recent establishment of the Old World pest Helicoverpa armigera (Hübner) into South America has had significant economic consequences and places the rest of the hemisphere at risk, emphasizing the need for improved methods of monitoring. A major complication is that a sibling species endemic to the New World, Helicoverpa zea (Boddie), is morphologically very similar, with the two species capable of producing fertile hybrids in the laboratory. The consequences of such hybridization in the field are uncertain, but could result in significant and unpredictable changes in the timing, range, and pesticide susceptibilities of Helicoverpa infestations...
October 2016: Journal of Economic Entomology
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