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https://www.readbyqxmd.com/read/29166561/structural-characterization-of-native-and-modified-encapsulins-as-nanoplatforms-for-in-vitro-catalysis-and-cellular-uptake
#1
Rindia M Putri, Carolina Allende-Ballestero, Daniel Luque, Robin Klem, Katerina-Asteria Rousou, Aijie Liu, Christoph H-H Traulsen, W Frederik Rurup, Melissa S T Koay, Jose R Castón, Jeroen J L M Cornelissen
Recent years have witnessed the emergence of bacterial semi-organelle encapsulins as promising platforms for bio-nanotechnology. To advance the development of encapsulins as nanoplatforms, a functional and structural basis of these assemblies is required. Encapsulin from Brevibacterium linens is known to be a protein-based vessel for an enzyme cargo in its cavity, which could be replaced with a foreign cargo resulting in a modified encapsulin. Here, we characterize the native structure of B. linens encapsulins with both native and foreign cargo using cryo-electron microscopy (cryo-EM)...
November 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/29166558/understanding-the-cellular-uptake-of-ph-responsive-zwitterionic-gold-nanoparticles-a-computer-simulation-study
#2
Xuebo Quan, Daohui Zhao, Libo Li, Jian Zhou
Surface functionalization of nanoparticles (NPs) with stealth polymers (e.g., hydrophilic and zwitterionic polymers) has become a common strategy to resist non-specific protein adsorption recently. Understanding the role of surface decoration on NP-biomembrane interactions is of great significance to promote the application of NPs in biomedical fields. Herein, using coarse-grained molecular dynamics (CGMD) simulations, we investigate the interactions between stealth polymer-coated gold nanoparticles (AuNPs) and lipid membranes...
November 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29166557/impact-of-crosslinker-valency-on-gold-nanoparticle-aggregate-formation-and-cellular-uptake
#3
Alice Liu, Jacob M Berlin
Synthesis of spherical, biocompatible nanoparticle aggregates using a small molecular crosslinker is a simple and flexible approach for the controlled assembly of gold nanoparticles. This strategy can be extended to a variety of crosslinkers, making it possible to the test the effect of crosslinker properties on aggregate formation and physicochemical properties. Here we synthesized aggregates using a series of structurally homologous crosslinkers with differing valencies. These aggregates have the same size, morphology, surface charge, surface coating, and stability in salt, media, and low pH conditions, but they differ in their stability to cyanide etching and uptake by cells...
November 22, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29165384/solid-lipid-nanoparticles-of-albendazole-for-enhancing-cellular-uptake-and-cytotoxicity-against-u-87-mg-glioma-cell-lines
#4
Gregory Marslin, Karthik Siram, Xiang Liu, Vinoth Kumar Megraj Khandelwal, Shen Xiaolei, Wang Xiang, Gregory Franklin
Albendazole (ABZ) is an antihelminthic drug used for the treatment of several parasitic infestations. In addition to this, there are reports on the anticancer activity of ABZ against a wide range of cancer types. However, its effect on glioma has not yet been reported. In the present study, cytotoxicity of ABZ and ABZ loaded solid lipid nanoparticles (ASLNs) was tested in human glioma/astrocytoma cell line (U-87 MG). Using glyceryl trimyristate as lipid carrier and tween 80 as surfactant spherical ASLNs with an average size of 218...
November 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29163910/cholesterol-directed-nanoparticle-assemblies-based-on-single-amino-acid-peptide-mutations-activate-cellular-uptake-and-decrease-tumor-volume
#5
Shang Li, Rongfeng Zou, Yaoquan Tu, Junchen Wu, Markita P Landry
Peptide drugs have been difficult to translate into effective therapies due to their low in vivo stability. Here, we report a strategy to develop peptide-based therapeutic nanoparticles by screening a peptide library differing by single-site amino acid mutations of lysine-modified cholesterol. Certain cholesterol-modified peptides are found to promote and stabilize peptide α-helix formation, resulting in selectively cell-permeable peptides. One cholesterol-modified peptide self-assembles into stable nanoparticles with considerable α-helix propensity stabilized by intermolecular van der Waals interactions between inter-peptide cholesterol molecules, and shows 68...
November 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29163827/fluorescence-contrast-enhanced-proliferative-lesion-imaging-by-enema-administration-of-indocyanine-green-in-a-rat-model-of-colon-carcinogenesis
#6
Nobuhiko Onda, Reiko Mizutani-Morita, Susumu Yamashita, Rei Nagahara, Shinya Matsumoto, Toshinori Yoshida, Makoto Shibutani
The fluorescent contrast agent indocyanine green (ICG) is approved by the Food and Drug Administration for clinical applications. We previously reported that cultured human colon tumor cells preferentially take up ICG by endocytic activity in association with disruption of their tight junctions. The present study explored ICG availability in fluorescence imaging of the colon to identify proliferative lesions during colonoscopy. The cellular uptake of ICG in cultured rat colon tumor cells was examined using live-cell imaging...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163792/dual-targeting-nanoparticles-with-excellent-gene-transfection-efficiency-for-gene-therapy-of-peritoneal-metastasis-of-colorectal-cancer
#7
Ling Li, Rui Deng, Yi Su, Cheng Yang
Colorectal cancer has been one of the most common cancers in the worldwide. Poor patient compliance and serious side effects often associated with conventional therapy (e.g. surgery, radiation, and chemotherapy). Gene therapy may be an alternative strategy. Herein, we developed a dual-targeting nanoparticle with excellent gene transfection efficiency for gene therapy of peritoneal metastasis of colorectal cancer. This nanoparticle can facilitate efficient cellular uptake and promote penetration into nucleus...
October 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/29163502/regulating-immunogenicity-and-tolerogenicity-of-bone-marrow-derived-dendritic-cells-through-modulation-of-cell-surface-glycosylation-by-dexamethasone-treatment
#8
Kevin Lynch, Oliver Treacy, Jared Q Gerlach, Heidi Annuk, Paul Lohan, Joana Cabral, Lokesh Joshi, Aideen E Ryan, Thomas Ritter
Dendritic cellular therapies and dendritic cell vaccines show promise for the treatment of autoimmune diseases, the prolongation of graft survival in transplantation, and in educating the immune system to fight cancers. Cell surface glycosylation plays a crucial role in the cell-cell interaction, uptake of antigens, migration, and homing of DCs. Glycosylation is known to change with environment and the functional state of DCs. Tolerogenic DCs (tDCs) are commonly generated using corticosteroids including dexamethasone, however, to date, little is known on how corticosteroid treatment alters glycosylation and what functional consequences this may have...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29163481/pro-resolving-mediators-in-regulating-and-conferring-macrophage-function
#9
REVIEW
Jesmond Dalli, Charles N Serhan
Macrophages are central in coordinating the host response to both sterile and infective insults. Clearance of apoptotic cells and cellular debris is a key biological action preformed by macrophages that paves the way to the resolution of local inflammation, repair and regeneration of damaged tissues, and re-establishment of function. The essential fatty acid-derived autacoids termed specialized pro-resolving mediators (SPM) play central roles in promoting these processes. In the present article, we will review the role of microvesicles in controlling macrophage efferocytosis and SPM production...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29162478/liposomes-assembled-from-dimeric-retinoic-acid-phospholipid-with-improved-pharmacokinetic-properties
#10
Lu Lu, Yawei Du, Muhammad Ismail, Longbing Ling, Chen Yao, Zhenglin Fu, Xinsong Li
All-trans-retinoic acid (ATRA) exhibits potent cytotoxicities against different cancer cells by binding to retinoic acid receptors (RARs), which is regarded as the first example of targeted therapy in acute promyelocytic leukemia (APL). However, its extensive clinical applications have been limited because of poor aqueous solubility, short half-life time and side effects. In this report, dimeric ATRA phosphorylcholine prodrug (Di-ATRA-PC) was designed and assembled into nanoliposomes to improve its pharmacokinetic properties...
November 18, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29161615/more-than-a-tad-spatiotemporal-control-of-caulobacter-pili
#11
REVIEW
Johann Mignolet, Gaël Panis, Patrick H Viollier
The Type IV pilus (T4P) is a powerful and sophisticated bacterial nanomachine involved in numerous cellular processes, including adhesion, DNA uptake and motility. Aside from the well-described subtype T4aP of the Gram-negative genera, including Myxococcus, Pseudomonas and Neisseria, the Tad (tight adherence) pilus secretion system re-shuffles homologous parts from other secretion systems along with uncharacterized components into a new type of protein translocation apparatus. A representative of the Tad apparatus, the Caulobacter crescentus pilus assembly (Cpa) machine is built exclusively at the newborn cell pole once per cell cycle...
November 18, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29161013/intelligent-peptide-gathering-mechanical-arm-tames-wild-trojan-horse-peptides-for-the-controlled-delivery-of-cancer-nanotherapeutics
#12
Nian-Qiu Shi, Yan Li, Yong Zhang, Nan Shen, Ling Qi, Shu-Ran Wang, Xian-Rong Qi
Cell-penetrating peptides (CPPs), also called "Trojan-Horse" peptides, have been used for facilitating intracellular delivery of numerous diverse cargoes and even nanocarriers. However, the lack of targeting specificity ("wildness" or nonselectivity) of CPP-nanocarriers remains an intractable challenge for many in vivo applications. In this work, we used an intelligent "peptide-gathering mechanical arm" (Int PMA) to curb CPPs' wildness and enhance the selectivity of R9-liposome-based cargo delivery for tumor targeting...
November 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29160121/near-infrared-light-responsive-nanoparticles-for-improved-anticancer-efficacy-through-synergistic-chemo-photothermal-therapy
#13
Yanli Li, Bing Xin Xiao, Jie Dong, Yun Liu, Shan Gao, Jie Pang, Zengxian Sun
Combined treatment is more effective than single treatment against most forms of cancer. The synergistic chemo-thermotherapy mediated by nanoparticles has superior advantages of lesser adverse effects as a promising cancer therapy modality. In this study, we report a theranostic carrier system co-encapsulating Doxorubicin (DOX) and Indocyanine green (ICG) into the D-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS). Full physicochemical characterization studies of the DOX/ICG-loaded TPGS nanoparticles (TNPs) are performed...
November 21, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/29159877/crystalline-structure-of-pulverized-dental-calculus-induces-cell-death-in-oral-epithelial-cells
#14
S M Ziauddin, A Yoshimura, J L Montenegro Raudales, Y Ozaki, K Higuchi, T Ukai, T Kaneko, T Miyazaki, E Latz, Y Hara
BACKGROUND AND OBJECTIVE: Dental calculus is a mineralized deposit attached to the tooth surface. We have shown that cellular uptake of dental calculus triggers nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation, leading to the processing of the interleukin-1β precursor into its mature form in mouse and human phagocytes. The activation of the NLRP3 inflammasome also induced a lytic form of programmed cell death, pyroptosis, in these cells...
November 20, 2017: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29159872/the-dark-side-of-glucose-transporters-in-prostate-cancer-are-they-a-new-feature-to-characterize-carcinomas
#15
REVIEW
Pedro Gonzalez-Menendez, David Hevia, Juan C Mayo, Rosa M Sainz
One of the hallmarks of cancer cells is the increased ability to acquire nutrients, particularly glucose and glutamine. Proliferating cells need precursors for cell growth and NADPH reducing equivalents for survival. The principal responsible for glucose uptake is facilitative glucose transporters (GLUTs), which usually are overexpressed in cancer cells. Besides their role in glucose uptake, GLUT transporters are able to transport other compounds such as dehydroascorbic acid or uric acid. They play a major role in tumor progression and cellular processes such as regulated cell death...
November 21, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29159749/fullerenol-based-intracellular-delivery-of-methotrexate-a-water-soluble-nanoconjugate-for-enhanced-cytotoxicity-and-improved-pharmacokinetics
#16
Shradha Bahuguna, Manish Kumar, Gajanand Sharma, Rajendra Kumar, Bhupinder Singh, Kaisar Raza
Derivatization of fullerenes to polyhydroxylated fullerenes, i.e., fullerenols (FLU), dramatically decreases their toxicity and has been reported to enhance the solubility as well as cellular permeability. In this paper, we report synthesis of FLU as nanocarrier and subsequent chemical conjugation of Methotrexate (MTX) to FLU with a serum-stable and intracellularly hydrolysable ester bond between FLU and MTX. The conjugate was characterized for physiochemical attributes, micromeritics, drug-loading, and drug-release and evaluated for cancer cell-toxicity, cellular-uptake, hemocompatibility, protein binding, and pharmacokinetics...
November 20, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/29159202/a-humoral-immune-response-alters-the-distribution-of-enzyme-replacement-therapy-in-murine-mucopolysaccharidosis-type-i
#17
Steven Q Le, Shih-Hsin Kan, Don Clarke, Valentina Sanghez, Martin Egeland, Kristen N Vondrak, Terence M Doherty, Moin U Vera, Michelina Iacovino, Jonathan D Cooper, Mark S Sands, Patricia I Dickson
Antibodies against recombinant proteins can significantly reduce their effectiveness in unanticipated ways. We evaluated the humoral response of mice with the lysosomal storage disease mucopolysaccharidosis type I treated with weekly intravenous recombinant human alpha-l-iduronidase (rhIDU). Unlike patients, the majority of whom develop antibodies to recombinant human alpha-l-iduronidase, only approximately half of the treated mice developed antibodies against recombinant human alpha-l-iduronidase and levels were low...
March 16, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29158843/ph-responsive-aerobic-nanoparticles-for-effective-photodynamic-therapy
#18
Lingyue Shen, Yu Huang, Dong Chen, Feng Qiu, Chuan Ma, Xin Jin, Xinyuan Zhu, Guoyu Zhou, Zhiyuan Zhang
RATIONALE: Photodynamic therapy (PDT), an O2-dependent treatment for inhibition of cancer proliferation, suffers from the low therapeutic effect in clinical application due to the hypoxic microenvironment in tumor cells. METHODS: To overcome this obstacle, a stimuli-responsive drug delivery system with O2 self-sufficiency for effective PDT was developed. In this study, pH-responsive aerobic nanoparticles were prepared by the electrostatic interaction between the O2-evolving protein Catalase and Chitosan...
2017: Theranostics
https://www.readbyqxmd.com/read/29158837/temperature-sensitive-gold-nanoparticle-coated-pluronic-pll-nanoparticles-for-drug-delivery-and-chemo-photothermal-therapy
#19
Ying Sun, Qi Wang, Jianhua Chen, Lei Liu, Li Ding, Ming Shen, Jin Li, Baoshan Han, Yourong Duan
Gold nanoparticle-coated Pluronic-b-poly(L-lysine) nanoparticles (Pluronic-PLL@Au NPs) were synthesized via an easy one-step method and employed as carriers for the delivery of paclitaxel (PTX) in chemo-photothermal therapy, in which Pluronic-PLL acts as the reductant for the formation of AuNPs without the need for an additional reducing agent. METHODS: The deposition of AuNPs on the surface of Pluronic-PLL micelles and the thermal response of the system were followed via ultraviolet-visible spectroscopy and dynamic light scattering...
2017: Theranostics
https://www.readbyqxmd.com/read/29158820/ph-sensitive-nano-complexes-overcome-drug-resistance-and-inhibit-metastasis-of-breast-cancer-by-silencing-akt-expression
#20
Jieying Yin, Tianqun Lang, Dongmei Cun, Zhong Zheng, Yan Huang, Qi Yin, Haijun Yu, Yaping Li
The therapy of breast cancer is encumbered by drug resistance and metastasis, which can be due to a defective PI3K/AKT/mTOR signaling pathway. This study was aimed at improving the anti-cancer effect of the chemotherapeutic agent paclitaxel (PTX) on the drug resistant and metastatic breast cancer by co-delivering PTX and a siRNA, siAkt, directed at silencing the Akt expression. Methods: The pH-sensitive amphiphilic polymer, poly [(1,4-butanediol)-diacrylate-β-N, N-diisopropylethylenediamine]-polyethyleneimine (BDP) was synthesized...
2017: Theranostics
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