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https://www.readbyqxmd.com/read/29793073/bubble-generating-polymersomes-loaded-with-both-indocyanine-green-and-doxorubicin-for-effective-chemotherapy-combined-with-photothermal-therapy
#1
Dunwan Zhu, Fan Fan, Chenlu Huang, Zhiming Zhang, Yu Qin, Li Lu, Hai Wang, Xu Jin, Hanxue Zhao, Hu Yang, Chao Zhang, Jun Yang, Zhipeng Liu, Hongfan Sun, Xigang Leng, Deling Kong, Linhua Zhang
The combination of chemotherapy and photothermaltherapy (PTT) via stimuli-responsive nanovesicles has great potential in tumor treatment. In the present study, bubble-generating polymersomes, which can generate bubbles in response to low pH or hyperthermia, were fabricated to simultaneously encapsulate chemotherapeutic drug and photosensitizing agent for the synergistic chemo-photothermal tumor therapy. Photosensitizer indocyanine green (ICG) was encapsulated into the bilayer of polymersomes formed by amphiphilic triblock copolymer PCL8000 -PEG8000 -PCL8000 through thin film re-hydration method, while chemotherapeutic doxorubicin (DOX) was loaded into the hydrophilic lumen using a transmembrane ammonium bicarbonate gradient loading procedure...
May 21, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29793055/fatty-acid-activation-in-thermogenic-adipose-tissue
#2
REVIEW
Sandra Steensels, Baran A Ersoy
Channeling carbohydrates and fatty acids to thermogenic tissues, including brown and beige adipocytes, have garnered interest as an approach for the management of obesity-related metabolic disorders. Mitochondrial fatty acid oxidation (β-oxidation) is crucial for the maintenance of thermogenesis. Upon cellular fatty acid uptake or following lipolysis from triglycerides (TG), fatty acids are esterified to coenzyme A (CoA) to form active acyl-CoA molecules. This enzymatic reaction is essential for their utilization in β-oxidation and thermogenesis...
May 21, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29792348/cell-division-cycle-7-kinase-is-a-negative-regulator-of-cell-mediated-collagen-degradation
#3
Michael Jeffrey Podolsky, Deepti Gupta, Arnold Ha, Ryan Ta, Amin Khalifeh-Soltani, William McKleroy, Ritwik Datta, Dean Sheppard, Kamran Atabai
Although extensive work has delineated many of the mechanisms of extracellular matrix (ECM) production, far less is known about pathways that regulate ECM degradation. This is particularly true of cellular internalization and degradation of matrix, which play an underappreciated role in ECM metabolism and lung fibrosis. For example, genetic perturbation of this pathway leads to exacerbated fibrosis in experimental animal models. In this work, we present the results of an unbiased screen of Drosophila phagocytes that yielded multiple genes that when silenced led to increased collagen uptake...
May 24, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29792261/structure-based-analysis-of-cysz-mediated-cellular-uptake-of-sulfate
#4
Zahra Assur Sanghai, Qun Liu, Oliver B Clarke, Meagan Belcher-Dufrisne, Pattama Wiriyasermkul, M Hunter Giese, Edgar Leal-Pinto, Brian Kloss, Shantelle Tabuso, James Love, Marco Punta, Surajit Banerjee, Kanagalaghatta R Rajashankar, Burkhard Rost, Diomedes Logothetis, Matthias Quick, Wayne A Hendrickson, Filippo Mancia
Sulfur, most abundantly found in the environment as sulfate (SO4 2- ), is an essential element in metabolites required by all living cells, including amino acids, co-factors and vitamins. However, current understanding of the cellular delivery of SO4 2- at the molecular level is limited. CysZ has been described as a SO4 2- permease, but its sequence family is without known structural precedent. Based on crystallographic structure information, SO4 2- binding and flux experiments, we provide insight into the molecular mechanism of CysZ-mediated translocation of SO4 2- across membranes...
May 24, 2018: ELife
https://www.readbyqxmd.com/read/29792071/phosphoramidates-and-phosphonamidates-protides-with-antiviral-activity
#5
Magdalena Slusarczyk, Michaela Serpi, Fabrizio Pertusati
Following the first report on the nucleoside phosphoramidate (ProTide) prodrug approach in 1990 by Chris McGuigan, the extensive investigation of ProTide technology has begun in many laboratories. Designed with aim to overcome limitations and the key resistance mechanisms associated with nucleoside analogues used in the clinic (poor cellular uptake, poor conversion to the 5'-monophosphate form), the ProTide approach has been successfully applied to a vast number of nucleoside analogues with antiviral and anticancer activity...
January 2018: Antiviral Chemistry & Chemotherapy
https://www.readbyqxmd.com/read/29791802/the-power-of-shielding-low-toxicity-and-high-transfection-performance-of-cationic-graft-copolymers-containing-poly-2-oxazoline-side-chains
#6
Anne-Kristin Trützschler, Tanja Bus, Martin Sahn, Anja Traeger, Christine Weber, Ulrich S Schubert
In this study we show the potential of oligo(2-ethyl-2-oxazoline) (OEtOx) shielded graft copolymers of (2 aminoethyl)-methacrylate (AEMA) and N methyl (2-aminoethyl) methacrylate (MAEMA) for pDNA delivery in HEK cells. Copolymers were synthesized via the macromonomer method using a combination of cationic ring opening polymerization (CROP) and reversible addition-fragmentation chain transfer (RAFT) polymerization to vary the degree of grafting (DG = 10% and 30%) as well as the side chain degree of polymerization (DP = 5 and 20)...
May 23, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29791241/neutrophil-mediated-delivery-of-pixantrone-loaded-liposomes-decorated-with-poly-sialic-acid-octadecylamine-conjugate-for-lung-cancer-treatment
#7
Xiang Luo, Ling Hu, Huangliang Zheng, Mingqi Liu, Xinrong Liu, Cong Li, Qiujun Qiu, Zitong Zhao, Xiaobo Cheng, Chaoyang Lai, Yuqing Su, Yihui Deng, Yanzhi Song
Poly(sialic acid) (PSA) is a natural hydrophilic biodegradable and non-immunogenic biopolymer, receptors for its monomer are expressed on peripheral blood neutrophils (PBNs), which plays important roles in the progression and invasion of tumors. A poly(sialic acid)-octadecylamine conjugate (PSA-ODA) was synthesized and then anchor it on the surface of liposomal pixantrone (Pix-PSL), to achieve an improved anticancer effect. The liposomes were prepared using a remote loading method via a pH gradient, and then assessed for particle size, zeta potential encapsulation efficiency, in vitro release, and in vitro cytotoxicity...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29791203/short-term-thermoneutral-housing-alters-glucose-metabolism-and-markers-of-adipose-tissue-browning-in-response-to-a-high-fat-diet-in-lean-mice
#8
Zachary Stephen Clayton, Carrie E McCurdy
Systemic insulin resistance and glucose intolerance occur with as little as 3 days of a high-fat diet (HFD) in mice and humans; the mechanisms that initiate acute insulin resistance are unknown. Most laboratories house mice at 22{degree sign}C, which is below their thermoneutral temperature (~30{degree sign}C). Cold stress has been shown to increase white adipose tissue (WAT) browning, alter lipid trafficking and impair immune function, while energy intake and expenditure decrease with increasing ambient temperature; importantly, dysregulation of these parameters have been strongly linked to obesity-induced insulin resistance...
May 23, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29790797/curcumin-loaded-redox-responsive-mesoporous-silica-nanoparticles-for-targeted-breast-cancer-therapy
#9
Nana Li, Zhi Wang, Yongtai Zhang, Kai Zhang, Jianxu Xie, Ying Liu, Wansi Li, Nianping Feng
HYPOTHESIS: The antitumor applications of curcumin (CUR) are limited because of its low water solubility, poor stability, and low bioavailability. We developed novel nanocarrier systems for tumour targeting and controlled CUR release and evaluated their therapeutic efficacy. EXPERIMENTS: The surface of mesoporous silica nanoparticles (MSN) was modified with hyaluronan (HA) or polyethyleneimine-folic acid (PEI-FA) via disulfide bonds. The capacity of the resultant nanocarriers (MSN-HA and MSN-PEI-FA, respectively) for CUR delivery was evaluated in a breast cancer cell line and a mouse xenograft model...
May 23, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29790795/octa-arginine-modified-poly-amidoamine-dendrimers-for-improved-delivery-and-cytotoxic-effect-of-paclitaxel-in-cancer
#10
Sri Vishnu Kiran Rompicharla, Preeti Kumari, Balaram Ghosh, Swati Biswas
Cell penetrating peptides (CPP) have the ability to penetrate the cell membrane and have been associated with various cargos for their facile intracellular translocation. The current study involves the synthesis of a CPP, octa-arginine (R8)-modified poly(amidoamine) dendrimer of generation 4 (G4), which has additionally been PEGylated and conjugated to the poorly soluble anticancer drug, paclitaxel (PTX). The synthesized dendrimer conjugates were characterized by proton nuclear magnetic resonance (1H-NMR) Spectroscopy and zeta potential measurements and evaluated in vitro in cell monolayers and 3D spheroids...
May 23, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29790399/mechanistic-insight-into-reactivity-and-geno-toxicity-of-well-characterized-nanoparticles-of-cobalt-metal-and-oxides
#11
Francesca Cappellini, Yolanda Hedberg, Sarah McCarrick, Jonas Hedberg, Remco Derr, Giel Hendriks, Inger Odnevall Wallinder, Hanna L Karlsson
An increasing use of cobalt (Co)-based nanoparticles (NPs) in different applications and exposures at occupational settings triggers the need for toxicity assessment. Improved understanding regarding the physiochemical characteristics of Co metal NPs and different oxides in combination with assessment of toxicity and mechanisms may facilitate decisions for grouping during risk assessment. The aim of this study was to gain mechanistic insights in the correlation between NP reactivity and toxicity of three different Co-based NPs (Co, CoO, and Co3 O4 ) by using various tools for characterization, traditional toxicity assays, as well as six reporter cell lines (ToxTracker) for rapid detection of signaling pathways of relevance for carcinogenicity...
May 23, 2018: Nanotoxicology
https://www.readbyqxmd.com/read/29790395/hyaluronic-acid-conjugated-ph-sensitive-liposomes-for-targeted-delivery-of-prednisolone-on-rheumatoid-arthritis-therapy
#12
Virgínia M Gouveia, José Lopes-de-Araújo, Sofia A Costa Lima, Cláudia Nunes, Salette Reis
AIM: The treatment of rheumatoid arthritis remains a challenge as available therapies still entail the risk of deleterious off-target effects. The present study describes hyaluronic acid-conjugated pH-sensitive liposomes as an effective drug delivery-targeting strategy to synovial cells. MATERIALS & METHODS: Therapeutic, cytotoxic and targeting potential of developed liposomes were studied in vitro using macrophages and fibroblasts cell lines. RESULTS & CONCLUSION: Results suggest an enhanced cellular uptake of conjugated liposomes, mainly mediated by caveolae- and clathrin-dependent endocytosis...
May 2018: Nanomedicine
https://www.readbyqxmd.com/read/29789819/exploring-the-cellular-uptake-and-localisation-of-phosphorescent-rhenium-fac-tricarbonyl-metallosurfactants-as-a-function-of-lipophilicity
#13
Andrew J Hallett, Emeline Placet, Roxane Prieux, Danielle McCafferty, James A Platts, David Lloyd, Marc Isaacs, Anthony J Hayes, Simon J Coles, Mateusz B Pitak, Sarah Marchant, Stephen N Marriott, Rudolf K Allemann, Athanasia Dervisi, Ian A Fallis
A systematic study of the cellular uptake of emissive complexes as a function of their lipophilicity is presented. Here a series of amphiphilic rhenium fac-tricarbonyl bisimine complexes bearing axial substituted imidazole or thiazole ligands, [Re(bpy)(CO)3(ImCnHm)]+ {n = 1 m = 3 (1+), n = 4 m = 9 (2+), n = 8 m = 17 (3+), n = 12 m = 25 (4+), n = 16 m = 33 (5+), n = 2 m = 3 (6+); bpy = 2,2'-bipyridine, Im = imidazole} and [Re(bpy)(CO)3(L)]+ {L = 1-mesitylimidazole, ImMes (7+), 4,5-dimethylthiazole, dmt (8+) and 4-methyl-5-thiazole-ethanol, mte (9+)} is reported...
May 23, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29789480/alterations-in-cellular-iron-metabolism-provide-more-therapeutic-opportunities-for-cancer
#14
REVIEW
Liangfu Zhou, Bin Zhao, Lixiu Zhang, Shenghang Wang, Dandan Dong, Huanhuan Lv, Peng Shang
Iron is an essential element for the growth and proliferation of cells. Cellular iron uptake, storage, utilization and export are tightly regulated to maintain iron homeostasis. However, cellular iron metabolism pathways are disturbed in most cancer cells. To maintain rapid growth and proliferation, cancer cells acquire large amounts of iron by altering expression of iron metabolism- related proteins. In this paper, normal cellular iron metabolism and the alterations of iron metabolic pathways in cancer cells were summarized...
May 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29788891/targeting-sugar-uptake-and-metabolism-for-cancer-identification-and-therapy-an-overview
#15
Marina Tanasova, Vagarshak V Begoyan, Lukasz J Weselinski
Metabolic deregulations have emerged as a cancer characteristic, opening a broad avenue for strategies and tools to target cancer through sugar uptake and metabolism. High expression levels of sugar transporters in cancer cells offered glycoconjugation as an approach to achieve enhanced cellular accumulation of drugs and imaging agents, with the sugar moiety anchoring the bioactive cargo to cancer cells. On the other hand, high demand for sugar nutrients in cancers provided a new avenue to target cancer cells with metabolic or sugar uptake inhibitors to induce cancer cells starvation or death...
May 22, 2018: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/29788824/activation-of-ampk-in-human-placental-explants-impairs-mitochondrial-function-and-cellular-metabolism
#16
Daphne Landau, Maricela Haghiac, Judi Minium, Yelenna Skomorovska-Prokvolit, Virtu Calabuig-Navarro, Perrie O'Tierney-Ginn
OBJECTIVE: Adenosine monophosphate-activated protein kinase (AMPK) is a cellular energy sensor whose phosphorylation increases energy production. We sought to evaluate the placenta-specific effect of AMPK activation on the handling of nutrients required for fetal development. METHODS: Explants were isolated from term placenta of 29 women (pregravid body mass index: 29.1 ± 9.9 kg/m2 ) and incubated for 24 hours with 0 to 100 µmol/L resveratrol or 0 to 1 mmol/L of 5-aminoimidazole-4-carboxyamide ribonucleoside (AICAR)...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29788158/cell-wall-bound-silicon-optimizes-ammonium-uptake-and-metabolism-in-rice-cells
#17
Huachun Sheng, Jie Ma, Junbao Pu, Lijun Wang
Background and Aims: Turgor-driven plant cell growth depends on cell wall structure and mechanics. Strengthening of cell walls on the basis of an association and interaction with silicon (Si) could lead to improved nutrient uptake and optimized growth and metabolism in rice (Oryza sativa). However, the structural basis and physiological mechanisms of nutrient uptake and metabolism optimization under Si assistance remain obscure. Methods: Single-cell level biophysical measurements, including in situ non-invasive micro-testing (NMT) of NH4+ ion fluxes, atomic force microscopy (AFM) of cell walls, and electrolyte leakage and membrane potential, as well as whole-cell proteomics using isobaric tags for relative and absolute quantification (iTRAQ), were performed...
May 16, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29787282/regulating-the-uptake-of-viral-nanoparticles-in-macrophage-and-cancer-cells-by-via-a-ph-switch
#18
Hamilton Lee, Candace E Benjamin, Chance M Nowak, Lana Tuong, Raymond P Welch, Zhuo Chen, Madushani Dharmarwardana, Kyle Murray, Leonidas Bleris, Sheena D'Arcy, Jeremiah J Gassensmith
Controlling the uptake of nanomaterials into phagocytes is a challenging problem. We describe an approach to inhibit the cellular uptake by macrophages and HeLa cells of nano-particles derived from bacteriophage Qβ by conjugating negatively charged terminal hexanoic acid moieties onto its surface. Additionally, we show hydrazone linkers can be installed between the surface of Qβ and the terminal hexa-noic acid moieties, resulting in a pH-responsive conjugate that, in acidic conditions, can release the terminal hexanoic acid moiety and allow for the uptake of the Qβ nanoparti-cle...
May 22, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29787265/synthesis-of-a-pillar-5-arene-based-polyrotaxane-for-enhancing-the-drug-loading-capacity-of-pcl-based-supramolecular-amphiphile-as-an-excellent-drug-delivery-platform
#19
Jianping He, Jianzhuang Chen, Shaoliang Lin, Dechao Niu, Jina Hao, Xiaobo Jia, Nan Li, Jinlou Gu, Yongsheng Li, Jianlin Shi
A pillar[5]arene-based nonionic polyrotaxane (PR) with star-poly(ε-caprolactone) (S-PCL) as the axle, pillar[5]arene (DEP5) as the wheel and adamantane as the end-capped group is designed and synthesized. The resulting PR is subsequently assembled with β-cyclodextrin end-capped pH-stimulated poly (acrylic acid) (CD-PAA) via a host-guest interaction to form the supramolecular pseudo-block polymer PR-PAA. This supramolecular pseudo-block polymer could self-assemble in aqueous solution to produce PR-PAA based supramolecular vesicular nanoparticles (PR-SVNPs), which presents significantly enhanced drug loading capacity (DLC, 45...
May 22, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29786715/cancer-cell-membrane-coated-magnetic-nanoparticles-for-mr-nir-fluorescence-dual-modal-imaging-and-photodynamic-therapy
#20
Jiong Li, Xuandong Wang, Dongye Zheng, Xinyi Lin, Zuwu Wei, Da Zhang, Zhuanfang Li, Yun Zhang, Ming Wu, Xiaolong Liu
Theranostic nanoprobes integrated with dual-modal imaging and therapeutic functions, such as photodynamic therapy (PDT), have exhibited significant potency in cancer treatments due to their high imaging accuracy and non-invasive advantages for cancer elimination. However, biocompatibility and highly efficient accumulation of these nanoprobes in tumor are still unsatisfactory for clinical application. In this study, a photosensitizer -loaded magnetic nanobead with surface further coated with a layer of cancer cell membrane (SSAP-Ce6@CCM) was designed to improve the biocompatibility and cellular uptake and ultimately achieve enhanced MR/NIR fluorescence imaging and PDT efficacy...
May 22, 2018: Biomaterials Science
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