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https://www.readbyqxmd.com/read/28945015/membrane-fusion-mediated-intracellular-delivery-of-lipid-bilayer-coated-mesoporous-silica-nanoparticles
#1
Jian Yang, Jing Tu, Gerda E M Lamers, René C L Olsthoorn, Alexander Kros
Protein delivery into the cytosol of cells is a challenging topic in the field of nanomedicine, because cellular uptake and endosomal escape are typically inefficient, hampering clinical applications. In this contribution cuboidal mesoporous silica nanoparticles (MSNs) containing disk-shaped cavities with a large pore diameter (10 nm) are studied as a protein delivery vehicle using cytochrome-c (cytC) as a model membrane-impermeable protein. To ensure colloidal stability, the MSNs are coated with a fusogenic lipid bilayer (LB) and cellular uptake is induced by a complementary pair of coiled-coil (CC) lipopeptides...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28944847/geniposide-promotes-autophagy-to-inhibit-insulin-resistance-in-hepg2-cells-via-p62-nf%C3%A2-%C3%AE%C2%BAb-glut%C3%A2-4
#2
Hongwei Jiang, Yujin Ma, Junqiang Yan, Jie Liu, Liping Li
Insulin resistance (IR) is known to be an important factor, which can lead to the onset of type 2 diabetes. Autophagy is a cellular process, which sequesters senescent or damaged proteins in autophagosomes for recycling of their products. Insulin and intracellular molecules, including mammalian target of rapamycin (mTOR), are well‑known inhibitors of autophagy. In patients with type 2 diabetes, the expression levels of glucose transporter 4 (GLUT‑4) in skeletal muscles are significantly decreased, indicating decreased glucose‑processing ability...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944765/folate-receptor-targeted-nir-sensitive-polydopamine-nanoparticles-for-chemo-photothermal-cancer-therapy
#3
Hao Li, Zhen Jin, Sunghoon Cho, Mi Jeong Jeon, Van Du Nguyen, Jong-Oh Park, Sukho Park
We propose the use of folate-receptor-targeted, near-infrared-sensitive polydopamine nanoparticles (NPs) for chemo-photothermal cancer therapy as an enhanced type of drug-delivery system which can be synthesized by in situ polymerization and conjugation with folic acid. The NPs consist of a Fe3O4/Au core, coated polydopamine, conjugated folic acid, and loaded anti-cancer drug (doxorubicin). The proposed multifunctional NPs show many advantages for therapeutic applications such as good biocompatibility and easy bioconjugation...
September 25, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28944661/tempo-conjugated-gold-nanoparticles-for-reactive-oxygen-species-scavenging-and-regulation-of-stem-cell-differentiation
#4
Jingchao Li, Jing Zhang, Ying Chen, Naoki Kawazoe, Guoping Chen
Controlling the differentiation of human mesenchymal stem cells (hMSCs) shows a great potential in regenerative medicine. Because overproduced reactive oxygen species (ROS) have obvious inhibitory effect on the differentiation and functions of hMSCs, it is highly desirable to develop effective strategy for ROS scavenging and stem cell differentiation controlling. In this study, gold nanoparticles (Au NPs) with an average size of 40 nm were conjugated with 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) to endow them with ROS scavenging capacity while holding the beneficial effect of Au NPs...
September 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28944006/regulation-of-gene-expression-is-associated-with-tolerance-of-the-arctic-copepod-calanus-glacialis-to-co2-acidified-sea-water
#5
Allison Bailey, Pierre De Wit, Peter Thor, Howard I Browman, Reidun Bjelland, Steven Shema, David M Fields, Jeffrey A Runge, Cameron Thompson, Haakon Hop
Ocean acidification is the increase in seawater pCO 2 due to the uptake of atmospheric anthropogenic CO 2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO 2, and associated decrease in pH, represents a climate change-related stressor. In this study, we investigated the gene expression patterns of nauplii of the Arctic copepod Calanus glacialis cultured at low pH levels. We have previously shown that organismal-level performance (development, growth, respiration) of C...
September 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28943206/kaolinite-in-pharmaceutics-and-biomedicine
#6
REVIEW
Mahmoud E Awad, Alberto López-Galindo, Massimo Setti, Mahmoud M El-Rahmany, César Viseras Iborra
Kaolinite Al2Si2O5(OH)4 is an abundant and inexpensive geomaterial regarded as one of the most common clay minerals in the earth's crust and the most widespread phase among the other kaolin polymorphs (halloysite, dickite and nacrite). Structurally, it is a hydrous aluminum phyllosilicate member belonging to the dioctahedral 1:1 kaolin mineral group. The particle size of the pseudohexagonal kaolinite platelets is normally <2μm (if compared to a human red blood cell of a typical diameter 6.2-8.2μm or to a virus particle of about 50nm diameter)...
September 21, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28943195/diels-alder-mediated-release-of-gemcitabine-from-hybrid-nanoparticles-for-enhanced-pancreatic-cancer-therapy
#7
Adeolu Oluwasanmi, Wejdan Al-Shakarchi, Ayesha Manzur, Mohammed H Aldebasi, Rayan S Elsini, Malek K Albusair, Katherine J Haxton, Anthony D M Curtis, Clare Hoskins
Hybrid nanoparticles (HNPs) have shown huge potential as drug delivery vehicles for pancreatic cancer. Currently, the first line treatment, gemcitabine, is only effective in 23.8% of patients. To improve this, a thermally activated system was developed by introducing a linker between HNPs and gemcitabine. Whereby, heat generation resulting from laser irradiation of the HNPs promoted linker breakdown resulting in prodrug liberation. In vitro evaluation in pancreatic adenocarcinoma cells, showed the prodrug was 4...
September 21, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28942644/an-endocannabinoid-uptake-inhibitor-from-black-pepper-exerts-pronounced-anti-inflammatory-effects-in-mice
#8
Inés Reynoso Moreno, Israel Najar-Guerrero, Noe Escareno, Mario Eduardo Flores, Jürg Gertsch, Juan Manuel Viveros-Paredes
Guineensine is a dietary N-isobutylamide widely present in black and long pepper (Piper nigrum and P. longum) previously shown to inhibit cellular endocannabinoid uptake. Given the role of endocannabinoids in inflammation and pain reduction, here we evaluated guineensine in mouse models of acute and inflammatory pain and endotoxemia. Significant dose-dependent anti-inflammatory effects (95.6 ± 3.1 % inhibition of inflammatory pain at 2.5 mg/kg i.p. and 50.0 ± 15.9 % inhibition of edema formation at 5 mg/kg i...
September 24, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28941799/regulatory-effects-of-simvastatin-and-apoj-on-app-processing-and-amyloid-%C3%AE-clearance-in-blood-brain-barrier-endothelial-cells
#9
Martina Zandl-Lang, Elham Fanaee-Danesh, Yidan Sun, Igor Čančar, Chaitanya Chakravarthi Gali, Alexandra Kober, Nicole M Albrecher, Carmen Tam-Amersdorfer, Anika Stracke, Steffen Storck, Ahmed Saeed, Jasminka Stefulj, Claus Pietrzik, Mark R Wilson, Ingemar Björkhem, Ute Panzenboeck
Amyloid-β peptides (Aβ) accumulate in cerebral capillaries indicating a central role of the blood-brain barrier (BBB) in the pathogenesis of Alzheimer's disease (AD). Although a relationship between apolipoprotein-, cholesterol- and Aβ metabolism is evident, the interconnecting mechanisms operating in brain capillary endothelial cells (BCEC) are poorly understood. ApoJ (clusterin) is present in HDL that regulates cholesterol metabolism which is disturbed in AD. ApoJ levels are increased in AD brains and in plasma of cerebral amyloid angiopathy (CAA) patients...
September 20, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28941283/intracellular-response-to-process-optimization-and-impact-on-productivity-and-product-aggregates-for-a-high-titer-cho-cell-process
#10
Michael W Handlogten, Allison Lee-O'Brien, Gargi Roy, Sophia V Levitskaya, Raghavan Venkat, Shailendra Singh, Sanjeev Ahuja
A key goal in process development for antibodies is to increase productivity while maintaining or improving product quality. During process development of an antibody, titers were increased from 4 to 10 g/L while simultaneously decreasing aggregates. Process development involved optimization of media and feed formulations, feed strategy, and process parameters including pH and temperature. To better understand how CHO cells respond to process changes, the changes were implemented in a stepwise manner. The first change was an optimization of the feed formulation, the second was an optimization of the medium, and the third was an optimization of process parameters...
September 23, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28941063/the-effects-of-physicochemical-properties-of-nanomaterials-on-their-cellular-uptake-in-vitro-and-in-vivo
#11
REVIEW
Ye Liu, Bereket Workalemahu, Xingyu Jiang
Understanding the cellular uptake of nanomaterials (NMs) is a fundamental and critical issue for uncovering biomedical effects of NMs and facilitating their practical applications in the clinic. In this Review, by highlighting the differences of NMs cellular uptake between in vitro and in vivo, a focus on reviewing recent works about the effects of four physicochemical parameters of NMs (size, rigidity, shape, surface modification) on regulating NMs cellular uptake in vitro and in vivo is undertaken. This review can provide readers some useful clues and new thinking for understanding NMs cellular uptake in depth...
September 20, 2017: Small
https://www.readbyqxmd.com/read/28940736/pressure-responsive-nanogel-base-on-alginate-cyclodextrin-with-enhanced-apoptosis-mechanism-for-colon-cancer-delivery
#12
Tolou Hosseinifar, Simin Sheybani, Majid Abdouss, Seyad Alireza Hassani Najafabadi, Mehdi Shafie Ardestani
5-folourouracil (5-Fu) commonly use in the treatment of different kinds of cancer, but limited cellular uptake and death is still a problem. Herein, we report a simple process for the synthesis of pressure-sensitive nanogels that indicate to be appropriate in the delivery of 5-Fu. The hydrogels (Al-CD) prepare by crosslinking of alginate (Al) with modified beta Cyclodextrin (β-CD) as Crosslinker. Next, nanoparticles obtaine by an emulsification method. 5-Fu as model drug loades into the Al-CD nanogels easily by mixing it in aqueous solution with the nanoparticles...
September 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28939611/concomitant-loss-of-the-glyoxalase-system-and-glycolysis-makes-the-uncultured-pathogen-candidatus-liberibacter-asiaticus-an-energy-scavenger
#13
Mukesh Jain, Alejandra Munoz-Bodnar, Dean W Gabriel
Methylglyoxal (MG) is a cytotoxic, non-enzymatic byproduct of glycolysis that readily glycates proteins and DNA, resulting in carbonyl stress. Glyoxalase I and II (GloA and GloB) sequentially convert MG into D-lactic acid using glutathione (GSH) as a cofactor. The glyoxalase system is essential for the mitigation of MG-induced carbonyl stress, preventing subsequent cell death, and recycling GSH for maintenance of cellular redox poise. All pathogenic Liberibacters identified to date are uncultured, including "Candidatus Liberibacter asiaticus", a psyllid endosymbiont and causal agent of the severely damaging citrus disease 'huanglongbing'...
September 22, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28939520/detailed-toxicity-evaluation-of-%C3%AE-cyclodextrin-coated-iron-oxide-nanoparticles-for-biomedical-applications
#14
Ruchita Shelat, Sudeshna Chandra, Aparna Khanna
The application of iron oxide nanoparticles [IONPs] in biomedical research is progressively increasing, leading to the rapid development of biocompatible and surface modified IONPs. However, there is still a need of information pertaining to its cellular and acute toxicity profile. This work reports the synthesis of β-cyclodextrin coated iron oxide nanoparticles (βCD-IONPs) and their characterization using spectroscopic (FT-IR), thermal (TGA) and surface analysis (TEM, SEM, BET and Zeta potential). All the characterization techniques displayed the synthesis of well dispersed, rod shaped βCD-IONPs of 45nm...
September 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28939002/phosphorogenic-sensors-for-biothiols-derived-from-cyclometalated-iridium-iii-polypyridine-complexes-containing-a-dinitrophenyl-ether-moiety
#15
Karson Ka-Shun Tso, Hua-Wei Liu, Kenneth Kam-Wing Lo
We report the synthesis and characterization of three cyclometalated iridium(III) polypyridine complexes containing a 2,4-dinitrophenyl ether moiety [Ir(pq)2(N^N)](PF6) (Hpq=2-phenylquinoline; N^N=4-(N-(4-(2,4-dinitrophenoxy)benzyloxy)carbonyl)aminomethyl-4'-methyl-2,2'-bipyridine (bpy-dinitro-1) (1a), 4-(2,4-dinitrophenoxy)methyl-4'-methyl-2,2'-bipyridine (bpy-dinitro-2) (2a), 4-(4-(2,4-dinitrophenoxy)phenyl)-2,2'-bipyridine (bpy-dinitro-3) (3a)) as intracellular sensors for biothiols. Due to the quenching effect of the dinitroaromatic moiety, these complexes were extremely weakly emissive...
September 12, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28938451/antibody-mediated-targeting-of-the-fgfr1c-isoform-increases-glucose-uptake-in-white-and-brown-adipose-tissue-in-male-mice
#16
Jo E Lewis, Ricardo J Samms, Scott Cooper, Jeni C Luckett, Alan C Perkins, James D Dunbar, Dennis P Smith, Paul J Emmerson, Andrew C Adams, Francis J P Ebling, Kostas Tsintzas
The increased prevalence of obesity and its cardiometabolic implications demonstrates the imperative to identify novel therapeutic targets able to effect meaningful metabolic changes in this population. Antibody-mediated targeting of fibroblast growth factor receptor 1c isoform (FGFR1c) has been shown to ameliorate hyperglycaemia and protect from diet- and genetically-induced obesity in rodents and non-human primates. However, it is currently unknown which tissue(s) contribute to this glucose lowering effect...
August 16, 2017: Endocrinology
https://www.readbyqxmd.com/read/28938430/role-of-the-bile-acid-transporter-slc10a1-in-liver-targeting-of-the-lipid-lowering-thyroid-hormone-analog-eprotirome
#17
Simone Kersseboom, Anja L M van Gucht, Alies van Mullem, Giulia Brigante, Stefania Farina, Bo Carlsson, Joanne M Donkers, Stan F J van de Graaf, Robin P Peeters, Theo J Visser
The thyroid hormone (TH) analog eprotirome (KB2115; 3-[[3,5-dibromo-4-[4-hydroxy-3-(1-methylethyl)-phenoxy]-phenyl]-amino]-3-oxopropanoic acid) was developed to lower cholesterol through selective activation of the T3 receptor isoform TRβ1 in the liver. Interestingly, eprotirome shows low uptake in non-hepatic tissues, explaining its lipid-lowering action without adverse extra-hepatic thyromimetic effects. Clinical trials have shown marked decreases in serum cholesterol levels.We explored the transport of eprotirome across the plasma membrane by members of three TH transporter families: monocarboxylate transporters MCT8 and 10, Na-independent organic anion transporters OATP1A2, 1B1, 1B3, 1C1, 2A1 and 2B1, and Na-dependent organic anion transporters SLC10A1-7...
August 17, 2017: Endocrinology
https://www.readbyqxmd.com/read/28937306/biocompatibility-assessment-of-functionalized-magnetic-mesoporous-silica-nanoparticles-in-human-heparg-cells
#18
Cédric Pisani, Estelle Rascol, Christophe Dorandeu, Clarence Charnay, Yannick Guari, Joël Chopineau, Jean-Marie Devoisselle, Odette Prat
Magnetic mesoporous silica nanoparticles (M-MSNs) are a promising class of nanoparticles for drug delivery. However, a deep understanding of the toxicological mechanisms of action of these nanocarriers is essential, especially in the liver. The potential toxicity on HepaRG cells of pristine, pegylated (PEG), and lipid (DMPC) M-MSNs were compared. Based on MTT assay and real-time cell impedance, none of these NPs presented an extensive toxicity on hepatic cells. However, we observed by transmission electron microscopy (TEM) that the DMPC and pristine M-MSNs were greatly internalized...
September 22, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/28937214/injectable-thermoresponsive-hydrogel-formed-by-alginate-g-poly-n-isopropylacrylamide-releasing-doxorubicin-encapsulated-micelles-as-smart-drug-delivery-system
#19
Min Liu, Xia Song, Yuting Wen, Jing-Ling Zhu, Jun Li
In this work, we have synthesized a thermoresponsive copolymer, alginate-g-poly(N-isopropylacrylamide) (alginate-g-PNIPAAm), by conjugating PNIPAAm to alginate, where PNIPAAm with different molecular weight and narrow molecular weight distribution was synthesized by atomic transfer radical polymerization (ATRP). The copolymer dissolved in water or PBS buffer solution at room temperature, and formed self-assembled micelles with low critical micellization concentrations when temperature increased to above their critical micellization temperatures...
September 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28935901/a-brain-targeting-lipidated-peptide-for-neutralizing-rna-mediated-toxicity-in-polyglutamine-diseases
#20
Qian Zhang, Mengbi Yang, Kasper K Sørensen, Charlotte S Madsen, Josephine T Boesen, Ying An, Shao Hong Peng, Yuming Wei, Qianwen Wang, Knud J Jensen, Zhong Zuo, Ho Yin Edwin Chan, Jacky Chi Ki Ngo
Polyglutamine (PolyQ) diseases are progressive neurodegenerative disorders caused by both protein- and RNA-mediated toxicities. We previously showed that a peptidyl inhibitor, P3, which binds directly to expanded CAG RNA can inhibit RNA-induced nucleolar stress and suppress RNA-induced neurotoxicity. Here we report a N-acetylated and C-amidated derivative of P3, P3V8, that showed a more than 20-fold increase in its affinity for expanded CAG RNA. The P3V8 peptide also more potently alleviated expanded RNA-induced cytotoxicity in vitro, and suppressed polyQ neurodegeneration in Drosophila with no observed toxic effects...
September 21, 2017: Scientific Reports
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