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https://www.readbyqxmd.com/read/28214920/detection-of-brucella-sp-infection-through-serological-microbiological-and-molecular-methods-applied-to-buffaloes-in-maranh%C3%A3-o-state-brazil
#1
Larissa Sarmento Dos Santos, Joicy Cortez Sá, Diego Luiz Dos Santos Ribeiro, Nancyleni Pinto Chaves, Juliana Pinto da Silva Mol, Renato Lima Santos, Tatiane Alves da Paixão, Alcina Vieira de Carvalho Neta
The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT)...
February 18, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28214536/t-cell-immunoregulation-in-active-ocular-toxoplasmosis
#2
Cynthia A Cordeiro, Erica L M Vieira, Vinicius M Castro, Walderez O Dutra, Rogerio A Costa, Juliana L Orefice, Wesley R Campos, Fernando Orefice, Lucy H Young, Antonio Lucio Teixeira
Toxoplasma gondii infection is an important cause of infectious ocular disease. The physiopathology of retinochoroidal lesions associated with this infection is not completely understood. The present study was undertaken to investigate cytokine production by T cells from individuals with active toxoplasmic retinochoroiditis (TR) comparing with controls. Eighteen patients with active TR and 15 healthy controls (6 controls IgG(+) to Toxoplasma and 9 negative controls) were included in the study. Peripheral blood mononuclear cells were incubated in the presence or absence of T...
February 15, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28214208/reduced-immunohistochemical-expression-of-adhesion-molecules-in-vitiligo-skin-biopsies
#3
Adriane Reichert Faria, Juliana Elizabeth Jung, Caio César Silva de Castro, Lucia de Noronha
Because defects in adhesion impairment seem to be involved in the etiopathogenesis of vitiligo, this study aimed to compare the immunohistochemical expression of several adhesion molecules in the epidermis of vitiligo and non lesional vitiligo skin. Sixty-six specimens of lesional and non lesional skin from 33 volunteers with vitiligo were evaluated by immunohistochemistry using anti-beta-catenin, anti-E-cadherin, anti-laminin, anti-beta1 integrin, anti-collagen IV, anti-ICAM-1 and anti-VCAM-1 antibodies. Biopsies of vitiligo skin demonstrated a significant reduction in the expression of laminin and integrin...
December 30, 2016: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28213765/association-between-signs-and-symptoms-of-bruxism-and-presence-of-tori-a-systematic-review
#4
Eduardo Bertazzo-Silveira, Juliana Stuginski-Barbosa, André Luís Porporatti, Bruce Dick, Carlos Flores-Mir, Daniele Manfredini, Graziela De Luca Canto
OBJECTIVE: This systematic review aims to answer the question: "Is there an association between any specific signs and symptoms of bruxism and the presence of tori?" MATERIAL AND METHODS: Observational studies, which evaluated the association between signs and symptoms of bruxism and tori, were selected. Signs and symptoms of bruxism (such as teeth grinding, jaw clenching, abnormal tooth wear, facial muscle hypertrophy, pain, or fatigue) had to be determined by questionnaire or anamnesis and tori within clinical assessment...
February 17, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28213584/using-the-biology-card-sorting-task-to-measure-changes-in-conceptual-expertise-during-postsecondary-biology-education
#5
Sarah A Bissonnette, Elijah D Combs, Paul H Nagami, Victor Byers, Juliana Fernandez, Dinh Le, Jared Realin, Selina Woodham, Julia I Smith, Kimberly D Tanner
While there have been concerted efforts to reform undergraduate biology toward teaching students to organize their conceptual knowledge like experts, there are few tools that attempt to measure this. We previously developed the Biology Card Sorting Task (BCST), designed to probe how individuals organize their conceptual biological knowledge. Previous results showed the BCST could differentiate between different populations, namely non-biology majors (NBM) and biology faculty (BF). In this study, we administered the BCST to three additional populations, using a cross-sectional design: entering biology majors (EBM), advanced biology majors (ABM), and biology graduate students (BGS)...
2017: CBE Life Sciences Education
https://www.readbyqxmd.com/read/28213050/reduced-frequency-of-two-activating-kir-genes-in-patients-with-sepsis
#6
Luciana M Oliveira, Pamela Portela, Joice Merzoni, Juliana D Lindenau, Fernando S Dias, Jaqueline Beppler, Pietra Graebin, Clarice S Alho, Gilberto Schwartsmann, Felipe Dal-Pizzol, Luiz Fernando Jobim, Mariana Jobim, Rafael Roesler
Natural killer (NK) cell activity is regulated by activating and inhibitory signals transduced by killer cell immunoglobulin-like receptors (KIR). Diversity in KIR gene repertoire among individuals may affect disease outcome. Sepsis development and severity may be influenced by genetic factors affecting the immune response. Here, we examined sixteen KIR genes and their human leucocyte antigen (HLA) class I ligands in critical patients, aiming to identify patterns that could be associated with sepsis. Male and female patients (ages ranging between 14 and 94 years-old) were included...
February 14, 2017: Human Immunology
https://www.readbyqxmd.com/read/28211323/evaluation-the-urban-atmospheric-conditions-in-different-cities-using-comet-and-micronuclei-assay-in-tradescantia-pallida
#7
Juliana Caroline Vivian Sposito, Bruno do Amaral Crispim, Amanda Izadora Romãn, Rosilda Mara Mussury, Joelson Gonçalves Pereira, Leonardo Oliveira Seno, Alexeia Barufatti Grisolia
In the present study, genotoxicity and mutagenicity were investigated in Tradescantia pallida exposed to vehicular traffic at different sites in a high-altitude tropical climate. During March, May, July, September, and November 2014, a comet assay and micronucleus bioassays were conducted on young inflorescences and leaves of T. pallida collected from twelve towns in the southern region of Mato Grosso do Sul with different amounts of vehicular traffic. Weather parameters (temperature, relative humidity and rainfall) were measured and vehicles were counted to determine traffic levels in each town...
February 1, 2017: Chemosphere
https://www.readbyqxmd.com/read/28210223/consumer-willingness-to-pay-for-dengue-vaccine-cyd-tdv-dengvaxia-%C3%A2-in-brazil-implications-for-future-pricing-considerations
#8
Isabella P Godói, André S Santos, Edna A Reis, Livia L P Lemos, Cristina M R Brandão, Juliana Alvares, Francisco A Acurcio, Brian Godman, Augusto A Guerra Júnior
Introduction and Objective: Dengue virus is a serious global health problem with an estimated 3.97 billion people at risk for infection worldwide. In December 2015, the first vaccine (CYD-TDV) for dengue prevention was approved in Brazil, developed by Sanofi Pasteur. However, given that the vaccine will potentially be paid via the public health system, information is need regarding consumers' willingness to pay for the dengue vaccine in the country as well as discussions related to the possible inclusion of this vaccine into the public health system...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28209722/long-term-outcome-of-hematopoietic-stem-cell-transplantation-for-il2rg-jak3-scid-a-cohort-report
#9
Intan Juliana Abd Hamid, Mary A Slatter, Fiona McKendrick, Mark S Pearce, Andrew R Gennery
Hematopoietic stem cell transplantation (HSCT) cures the T-lymphocyte, B-lymphocyte and Natural Killer (NK) cell differentiation defect in IL2RG/JAK3 SCID. We evaluated long-term clinical features, longitudinal immunoreconstitution, donor chimerism and quality of life (QoL) of IL2RG/JAK3 SCID patients >2 years post-HSCT at our center. Clinical data were collated and patients/families answered PedsQL Generic Core Scale v4.0 questionnaires. We performed longitudinal analyses of CD3+, CD4+ naïve T-lymphocyte, CD19+ and NK cell numbers from pre-transplant until 15 years post-transplant...
February 16, 2017: Blood
https://www.readbyqxmd.com/read/28209543/hpa-axis-and-inflammatory-reactivity-to-acute-stress-is-related-with-basal-hpa-axis-activity
#10
Xuejie Chen, Danielle Gianferante, Luke Hanlin, Alexander Fiksdal, Juliana G Breines, Myriam V Thoma, Nicolas Rohleder
INTRODUCTION: Inflammation is drawing attention as pathway between psychosocial stress and health, and basal HPA axis activity has been suggested to exert a consistent regulatory influence on peripheral inflammation. Here we studied the relationship between basal HPA axis activity and inflammatory and HPA axis acute stress reactivity. METHODS: We recruited 48 healthy individuals and collected saliva for diurnal cortisol sampling at 6 points. Participants were previously exposed to the Trier Social Stress Test (TSST) on two consecutive days...
February 6, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28209481/botulinum-toxin-type-a-reduces-inflammatory-hypernociception-induced-by-arthritis-in-the-temporomadibular-joint-of-rats
#11
Victor Ricardo Manuel Muñoz Lora, Juliana Trindade Clemente-Napimoga, Henrique Ballassini Abdalla, Cristina Gomes Macedo, Giancarlo de la Torre Canales, Celia Marissa Rizzatti Barbosa
OBJECTIVE: This study aimed to investigate the antinociceptive effects of Botulinum toxin type A (BoNT-A) on persistent inflammatory hypernociception induced by arthritis in the temporomandibular joint (TMJ) of rats. MATERIAL AND METHODS: Wistar rats were induced to persistent inflammatory hypernociception in the left TMJ. Then, animals were treated with intra-TMJ injections of BoNT-A, using doses of 3.5, 7 and 14 U/kg. Saline was used as control group. Behavioral tests were applied to evaluated the effect of BoNT-A in the inflammatory hypernociception...
February 13, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28209096/increased-oxidative-stress-alters-nucleosides-metabolite-levels-in-sickle-cell-anemia
#12
Lívia Gelain Castilhos, Juliana Sorraila de Oliveira, Stephen Adeniyi Adefegha, Luana Pereira Magni, Pedro Henrique Doleski, Fatima Husein Abdalla, Cínthia Melazzo de Andrade, Daniela Bitencourt Rosa Leal
OBJECTIVES: This study was conducted to assess the markers of oxidative stress, myeloperoxidase (MPO), acetylcholinesterase (AChE) and xanthine oxidase (XO) activities as well as the levels of nucleotide metabolites in sickle cell anemia (SCA) patients. METHODS: Fifteen SCA treated patients and 30 health subjects (control group) were selected. The markers of oxidative stress (levels of reactive oxygen species (ROS), plasma proteins, carbonyl content, lipid peroxidation (TBARS), total thiols (T-SH), glutathione and catalase activity), MPO, AChE and XO activities as well as the levels of nucleotide metabolites were measured in SCA patients...
February 16, 2017: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/28207897/correction-inhibitory-effects-of-hydroethanolic-leaf-extracts-of-kalanchoe-brasiliensis-and-kalanchoe-pinnata-crassulaceae-against-local-effects-induced-by-bothrops-jararaca-snake-venom
#13
Júlia Morais Fernandes, Juliana Félix-Silva, Lorena Medeiros da Cunha, Jacyra Antunes Dos Santos Gomes, Emerson Michell da Silva Siqueira, Luisa Possamai Gimenes, Norberto Peporine Lopes, Luiz Alberto Lira Soares, Matheus de Freitas Fernandes-Pedrosa, Silvana Maria Zucolotto
[This corrects the article DOI: 10.1371/journal.pone.0168658.].
2017: PloS One
https://www.readbyqxmd.com/read/28206857/phenotypic-plasticity-in-response-to-seasonal-variation-in-populations-of-culex-melanoconion-bastagarius
#14
Jeronimo Alencar, Cecilia Ferreira de Mello, Hélcio R Gil-Santana, Christina S da Silva, Juliana S Sarmento, Ana Laura Carbajal de La Fuente
The present study aimed to determine the effects of seasonal variation on phenotypic variation in Culex bastagarius, using geometric morphometric analysis based on wing shape and size. Samples were collected in the Guapiaçu Ecological Reserve, in Rio de Janeiro, Brazil. Mosquitoes were captured once every 2 months (December 2012-January 2014) using light traps. The results of geometric morphometric analysis revealed variations in wing size; however, variability was not evident in wing shape. The present study provides novel data regarding phenotypic plasticity of Cx...
December 2016: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28206769/extensive-chemical-modifications-in-the-primary-protein-structure-of-igg1-subvisible-particles-are-necessary-for-breaking-immune-tolerance
#15
Bjoern Boll, Juliana Bessa, Emilien Folzer, Anacelia Ríos Quiroz, Roland Schmidt, Patrick Bulau, Christof Finkler, Hanns-Christian Mahler, Jörg Huwyler, Antonio Iglesias, Atanas V Koulov
A current concern with the use of therapeutic proteins is the likely presence of aggregates and submicron, subvisible and visible particles. It has been proposed that aggregates and particles may lead to unwanted increases in immune response with a possible impact on safety or efficacy. The aim of this study was thus to evaluate the ability of subvisible particles of a therapeutic antibody to break immune tolerance in an IgG1 transgenic mouse model and to understand the particle attributes that may play a role in this process...
February 16, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28203303/refractory-carcinoid-syndrome-a-review-of-treatment-options
#16
REVIEW
Rachel P Riechelmann, Allan A Pereira, Juliana F M Rego, Frederico P Costa
Carcinoid syndrome (CSy) is a constellation of symptoms that may commonly present in patients with well differentiated neuroendocrine tumors (NETs), with somatostatin analogs (SSAs) being the first-line option for symptom management. However, symptomatic progression eventually occurs and in this scenario of a refractory CSy; several treatment options have been studied such as dose escalation of SSA, interferon and liver-directed therapies. Nevertheless, recent phase III trials have contributed to the understanding and management of this condition...
February 2017: Therapeutic Advances in Medical Oncology
https://www.readbyqxmd.com/read/28202455/in-the-interface-of-caesalpinioids-and-mimosoids-comparative-floral-development-elucidates-shared-characters-in-dimorphandra-mollis-and-pentaclethra-macroloba-leguminosae
#17
Thais C De Barros, Giseli D Pedersoli, Juliana V Paulino, Simone P Teixeira
PREMISE OF THE STUDY: Pentaclethra and Dimorphandra (Leguminosae) have long been considered a possible enigmatic link between caesalpinioids and mimosoids because they both have an imbricate calyx and heteromorphic androecium, floral features that are rare among mimosoids but common among caesalpinioids. This study compared the developing flowers of Dimorphandra mollis and Pentaclethra macroloba to determine whether the shared floral conditions also have the same ontogenetic origin. METHODS: Buds of different sizes and flowers were processed for surface (scanning electron microscopy) and histological (light microscopy) examination...
February 15, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28202316/an-epigenetic-modifier-induces-production-of-10-s-verruculide-b-an-inhibitor-of-protein-tyrosine-phosphatases-by-phoma-sp-nov-lg0217-a-fungal-endophyte-of-parkinsonia-microphylla
#18
Juliana R Gubiani, E M Kithsiri Wijeratne, Taoda Shi, Angela R Araujo, A Elizabeth Arnold, Eli Chapman, A A Leslie Gunatilaka
Incorporation of the histone deacetylase (HDAC) inhibitor, suberoylanilide hydroxamic acid (SAHA), to a culture broth of the endophytic fungus Phoma sp. nov. LG0217 isolated from Parkinsonia microphylla changed its metabolite profile and resulted in the production of (10'S)-verruculide B (1), vermistatin (2) and dihydrovermistatin (3). When cultured in the absence of the epigenetic modifier, it produced a new metabolite, (S,Z)-5-(3',4'-dihydroxybutyldiene)-3-propylfuran-2(5H)-one (4) together with nafuredin (5)...
February 3, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28202059/cardiosphere-derived-cells-do-not-improve-cardiac-function-in-rats-with-cardiac-failure
#19
Taís Hanae Kasai-Brunswick, Andréa Rodrigues da Costa, Raiana Andrade Quintanilha Barbosa, Bruna Farjun, Fernanda Cristina Paccola Mesquita, Danúbia Silva Dos Santos, Isalira Peroba Ramos, Grazielle Suhett, Guilherme Visconde Brasil, Sandro Torrentes da Cunha, José Oscar R Brito, Juliana do Amaral Passipieri, Adriana Bastos Carvalho, Antonio Carlos Campos de Carvalho
BACKGROUND: Heart failure represents an important public health issue due to its high costs and growing incidence worldwide. Evidence showing the regenerative potential of postmitotic heart tissue has suggested the existence of endogenous cardiac stem cells in adult hearts. Cardiosphere-derived cells (CDC) constitute a candidate pool of such cardiac stem cells. Previous studies using acute myocardial infarction (MI) models in rodents demonstrated an improvement in cardiac function after cell therapy with CDC...
February 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28199900/diagnosis-of-resistance-alleles-in-codon-167-of-the-beta-tubulin-cya-tbb-1-gene-from-third-stage-larvae-of-horse-cyathostomins
#20
Juliana Bana Ishii, Amilcar Arenal, Aline Felix, Ursula Yoshitani, Robin Beech, Marcelo Beltrão Molento
Anthelmintic resistance is a serious problem for the control of equine gastrointestinal nematodes. In the present survey, 173 third stage larvae of cyathostomins were investigated from three different locations for the presence of the resistant genotype at codon 167 of the beta-tubulin gene, as this is the most prevalent mutation. The larvae from the state of Parana (n=67), Sao Paulo (n=54) and Santa Catarina (n=52), showed 61.2; 31.5 and 38.5% of the heterozygous resistant genotype - TTC/TAC, respectively...
January 25, 2017: Research in Veterinary Science
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