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https://www.readbyqxmd.com/read/26554930/the-performance-of-the-four-anaerobic-blood-culture-bottles-bact-alert-fn-fn-plus-bactec-plus-and-lytic-in-detection-of-anaerobic-bacteria-and-identification-by-direct-maldi-tof-ms
#1
Mohammed Almuhayawi, Osman Altun, Adam Dilshad Abdulmajeed, Måns Ullberg, Volkan Özenci
Detection and identification of anaerobic bacteria in blood cultures (BC) is a well-recognized challenge in clinical microbiology. We studied 100 clinical anaerobic BC isolates to evaluate the performance of BacT/ALERT-FN, -FN Plus (BioMérieux), BACTEC-Plus and -Lytic (Becton Dickinson BioSciences) BC bottles in detection and time to detection (TTD) of anaerobic bacteria. BACTEC Lytic had higher detection rate (94/100, 94%) than BacT/ALERT FN Plus (80/100, 80%) (p<0.01) in the studied material. There was no significant difference in detection of anaerobic bacteria among the remaining bottle types...
2015: PloS One
https://www.readbyqxmd.com/read/26254850/performance-of-bact-alert-resin-based-fn-plus-bottles-compared-with-bact-alert-charcoal-based-fn-bottles-for-the-detection-of-anaerobes-in-experimentally-seeded-blood-cultures
#2
COMPARATIVE STUDY
Steven De Keukeleire, Ingrid Wybo, Kristof Emmerechts, Denis Piérard
Recently new resin-based BacT/Alert FAN Plus bottles containing antibiotic-binding polymeric beads, were introduced as an improvement of the charcoal-based FAN bottles for the recovery of bacteria and fungi. To assess the performance of the novel anaerobic FN plus bottles in the detection of anaerobic organisms, we compared the detection rate and the time to detection (TTD) in spiked resin-based FN Plus bottles and charcoal-based FN bottles. The bottles were experimentally seeded with reference strains or clinical strains collected from positive blood cultures...
October 2015: Anaerobe
https://www.readbyqxmd.com/read/25704776/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-maldi-tof-directly-from-positive-blood-culture-flasks-allows-rapid-identification-of-bloodstream-infections-in-immunosuppressed-hosts
#3
A Egli, M Osthoff, D Goldenberger, J Halter, S Schaub, J Steiger, M Weisser, R Frei
INTRODUCTION: In immunosuppressed hosts, rapid identification of microorganisms of bloodstream infections is crucial to ensuring effective antimicrobial therapy. Conventional culture requires up to 72 h from sample collection to pathogen identification. METHODS: We used the SepsiTyper Kit and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF; Microflex, Bruker) directly from positive blood culture (BacT/ALERT 3D, FN/FA vials; bioMérieux) in comparison to standard culture methodology (VITEK 2; bioMérieux) for species identification...
June 2015: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/25370703/evaluation-of-a-new-protocol-for-sterility-controls-of-corneal-culture-medium
#4
H Thomasen, F Mosel, K-P Steuhl, D Meller
Careful testing for microbial contamination is essential for corneal transplants. Sterility tests are performed on the antibiotics containing culture medium leaving the problem that antibiotics might compromise the test results. In this study a protocol for the application of the automated BacT/Alert system for sterility testing of corneal cell culture medium was examined. Corneal culture medium in combination with an antibiotics degrading enzyme were injected in resin containing test bottles of the BacT/Alert system named FA plus (intended for aerobic microorganisms) or FN plus (intended for anaerobic microorganisms) depending on their aerobic or anaerobic nature...
September 2015: Cell and Tissue Banking
https://www.readbyqxmd.com/read/25048395/the-first-case-of-human-protothecosis-caused-by-prototheca-zopfii-in-japan
#5
Mina Takano, Shuichiro Hoshi, Kumiko Nagai, Haruka Ishidaira, Masanobu Onozaki, Kazuo Satoh, Koichi Makimura
This report describes a fatal case Prototheca zopfii genotype 2 infection in an immunosuppressed patient. The patient was a 62-year-old housewife who presented general malaise in April 2011. Hairy cell leukemia was highly suspected. Chemotherapy was started because the patient developed severe pancytopenia in October 2011. Itraconazole capsules (100 mg/day) and trimethoprim (320 mg/day) plus sulfamethoxazole (1600 mg/day) combinations were orally administered for prophylaxis of fungal infections. Of BacT/ALERT 3D FA aerobic culture bottles and FN anaerobic culture bottles, only FA aerobic blood culture bottles produced positive reactions when the patient developed fever in January 2012...
October 2014: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/24371240/controlled-clinical-comparison-of-bact-alert-fa-plus-and-fn-plus-blood-culture-media-with-bact-alert-fa-and-fn-blood-culture-media
#6
MULTICENTER STUDY
T J Kirn, S Mirrett, L B Reller, M P Weinstein
New blood culture media containing antibiotic-binding polymeric beads have been developed for the BacT/Alert (bioMérieux, Inc., Durham, NC) blood culture system. To assess the performance of these new media, we compared the new BacT/Alert aerobic medium (FA Plus) with resins to BacT/Alert FA medium with activated charcoal and the new BacT/Alert anaerobic medium (FN Plus) to BacT/Alert FN medium at 3 tertiary care medical centers. Study bottle pairs were inoculated with a target volume of 6 to 10 ml of blood from adults and incubated in the BacT/Alert 3D blood culture instrument...
March 2014: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/24108609/clinical-evaluation-of-bact-alert-fa-plus-and-fn-plus-bottles-compared-with-standard-bottles
#7
COMPARATIVE STUDY
Dong-Hyun Lee, Seong Chun Kim, In-Gyu Bae, Eun-Ha Koh, Sunjoo Kim
The performance of the BacT/Alert FA Plus and FN Plus resin bottles was evaluated in comparison with that of standard aerobic (SA) and standard anaerobic (SN) bottles. Twenty milliliters of blood from adult patients was equally distributed into four types of bottles: FA Plus, FN Plus, SA, and SN. The detection of clinically significant organisms and the time to detection (TTD) were monitored for each bottle. Among the 3,103 blood culture sets that were requested, the blood volume of each bottle was over 4 ml in 1,481 sets (47...
December 2013: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/23486710/neutralization-of-antimicrobial-substances-in-new-bact-alert-fa-and-fn-plus-blood-culture-bottles
#8
Dieter Mitteregger, Wolfgang Barousch, Marion Nehr, Michael Kundi, Markus Zeitlinger, Athanasios Makristathis, Alexander M Hirschl
Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from blood culture media, media formulations and the blend of resins may influence performance. The BacT/Alert 3D system (bioMérieux) was investigated using the new resin-containing medium types FA Plus (aerobic) and FN Plus (anaerobic)...
May 2013: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/22669561/comparison-of-the-two-blood-culture-systems-bactec-9240-and-bact-alert-3d-in-the-detection-of-candida-spp-and-bacteria-with-polymicrobial-sepsis
#9
COMPARATIVE STUDY
L Klingspor, S A Muhammed, V Ozenci
The purpose of this investigation was to evaluate the performance of the Bactec 9240 and BacT/Alert 3D blood culture systems in the detection of Candida spp. and bacteria in simulated polymicrobial sepsis models. A total of 28 clinical isolates of Escherichia coli, Staphylococcus aureus, Candida albicans, and Candida glabrata were studied. Five polymicrobial models of C. albicans + S. aureus, C. albicans + E. coli, C. glabrata + S. aureus, C. glabrata + E. coli, and C. albicans + C. glabrata were prepared. Each combination was inoculated in five different blood culture vials...
November 2012: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/22541787/evaluation-of-bactec-plus-aerobic-and-anaerobic-blood-culture-bottles-and-bact-alert-fan-aerobic-and-anaerobic-blood-culture-bottles-for-the-detection-of-bacteremia-in-icu-patients
#10
COMPARATIVE STUDY
Kyoung Ho Roh, Ju Yeon Kim, Ha Nui Kim, Hye Jin Lee, Jang Wook Sohn, Min Ja Kim, Yunjung Cho, Young Kee Kim, Chang Kyu Lee
Blood culture is the most valuable laboratory test for the diagnosis of bacteremia and sepsis. The BACTEC FX and BacT/Alert 3D automated blood culture systems are commonly used in Korean health care facilities. A controlled clinical evaluation of the resin-containing BACTEC Plus aerobic (BA) and anaerobic (BN), and the charcoal-containing FAN aerobic (FA) and anaerobic (FN) bottles using blood from intensive care unit (ICU) patients was designed. The performances of these 2 systems with media containing particle absorbing antimicrobial agents were evaluated using the culture positivity rate and time to detection (TTD)...
July 2012: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/22494558/clinical-comparison-of-the-bactec-mycosis-ic-f-bact-alert-fa-and-bact-alert-fn-blood-culture-vials-for-the-detection-of-candidemia
#11
COMPARATIVE STUDY
Eva-Lena Ericson, Lena Klingspor, Måns Ullberg, Volkan Ozenci
The present study analyzed the performance of Bactec Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN vials in detection and time to detection (TTD) of Candida spp. in 179 simultaneous blood cultures. The Mycosis IC/F, BacT/Alert FA, and BacT/Alert FN vials could detect Candida spp. in 144 (80.45%) of 179, 149 (83.24%) of 179, and 8 (4.47%) of 179 samples, respectively. With the presence of antifungal therapy, the numbers of positive vials were higher in BacT/Alert FA compared to Mycosis IC/F, 87/99 versus 73/99, respectively (P < 0...
June 2012: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/19778301/performance-of-vitek-2-compact-and-overnight-microscan-panels-for-direct-identification-and-susceptibility-testing-of-gram-negative-bacilli-from-positive-fan-bact-alert-blood-culture-bottles
#12
M D Quesada, M Giménez, S Molinos, G Fernández, M D Sánchez, R Rivelo, A Ramírez, G Banqué, V Ausina
We describe the reliability of the VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing from the BacT/ALERT blood culture system when using FAN (FA and FN) bottles. A simple procedure, in two centrifugation steps, was designed to remove the charcoal particles present in FA and FN bottles. A total of 113 positive blood cultures showing Gram-negative rods were investigated. Enterobacteriaceae were isolated in 104 cases, and Pseudomonas aeruginosa in nine. The MicroScan system correctly identified 106 (93...
February 2010: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/16597846/effects-of-delayed-entry-conditions-on-the-recovery-and-detection-of-microorganisms-from-bact-alert-and-bactec-blood-culture-bottles
#13
R L Sautter, A R Bills, D L Lang, G Ruschell, B J Heiter, P P Bourbeau
Manufacturers generally recommend that blood culture bottles be loaded into instruments within a short time of collection. However, in our experience, delays often occur prior to loading the bottles. We examined the effect of holding bottles under various temperatures (T)-room temperature (RT), 4 degrees C, 37 degrees C, and RT for 2 h following incubation at 37 degrees C (to simulate transit [TR])-and for various holding times of 4, 12, and 24 h. We utilized the BacT/ALERT system with FA and FN bottles and the BACTEC system with Plus (PL) and Lytic 10 (LY) bottles...
April 2006: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/16306015/roadmap-to-approval-use-of-an-automated-sterility-test-method-as-a-lot-release-test-for-carticel-autologous-cultured-chondrocytes
#14
G Kielpinski, S Prinzi, J Duguid, G du Moulin
BACKGROUND: In February 2004, FDA approved a supplement to our biologics license for Carticel, autologous cultured chondrocytes, to use the BacT/ALERT microbial detection system as an alternative to the compendial sterility test for lot release. This article provides a roadmap to our approval process. The approval represents more than 4 years of development and validation studies comparing the Steritest compact system to the BacT/ALERT microbial detection system. METHODS: For this study, freshly cultured chondrocytes were prepared from a characterized cell bank...
2005: Cytotherapy
https://www.readbyqxmd.com/read/16025428/-comparison-of-the-detection-of-anaerobic-bacteria-in-haemoculture-systems-bactec-lytic-10-anaerobic-f-bact-alert-fan-anaerobic-and-bact-alert-fn-anaerobic
#15
COMPARATIVE STUDY
Pavel Cermák, Miroslav Förstl
The purpose of this study is to deal with the problem of anaerobic cultivation of clinical specimens and consider the possibility of using semi-automated blood culture instruments. The Bactec Lytic, BacT/Alert FAN Anaerobic and BacT/Alert FN Anaerobic bottles were inoculated with Bacteriodes fragilis, Clostridium perfringens, Peptostreptococcus anaerobius and Feingoldia magna strains. The times to detection (TTD) for positive bottles were evaluated with reference to the number of inoculated bacteria. Inoculation with the same suspension of equal bacterial strain resulted in shorter TTD values for all the Bactec Lytic bottles as compared with the BacT/Alert FAN Anaerobic or BacT/Alert FN Anaerobic bottles, respectively...
June 2005: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/16000442/comparative-recovery-of-microorganisms-from-bact-alert-plastic-and-glass-fa-and-fn-blood-culture-bottles
#16
COMPARATIVE STUDY
J A Riley, B J Heiter, P P Bourbeau
bioMerieux, Inc., has recently introduced plastic bottles to replace glass bottles for use in the BacT/ALERT blood culture system. We compared the performance of the plastic to the glass bottles in a large clinical evaluation. Two blood cultures were collected from each patient, one using glass FA (aerobic) and FN (anaerobic) bottles and one using plastic FA and FN bottles. Of the 4,040 sets of four bottles collected, 3,110 contained the recommended 8 to 12 ml of blood, yielding 524 microorganisms with 359 judged to be clinically significant...
July 2005: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/15995643/evaluation-of-negative-results-of-bact-alert-3d-automated-blood-culture-system
#17
M Esra Kocoglu, Aysen Bayram, Iclal Balci
Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (FA) and anaerobic (FN) [corrected] media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in 5% sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar...
June 2005: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/15872297/routine-incubation-of-bact-alert-fa-and-fn-blood-culture-bottles-for-more-than-3-days-may-not-be-necessary
#18
Paul P Bourbeau, Michael Foltzer
We reviewed time to detection for 35,500 blood cultures collected in BacT/ALERT FA and FN bottles. In the first 3 days of incubation, 97.5% of the 2,609 clinically significant isolates were detected, suggesting that routine incubation for more than 3 days may not be necessary for FA and FN bottles.
May 2005: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/15628528/-effect-of-delay-of-blood-cultures-on-positive-detection-by-automated-blood-culture-system
#19
Intetsu Kobayashi, Mariko Yamamoto, Miyuki Hasegawa, Yumie Sato, Utsuki Uchino, Akihiro Kaneko
The effect of entry delayed blood culture bottles until the start of incubation for mechanical detection of organism were compared using 2 major blood culture systems; BACTEC 9240 system and BacT/ALERT 3D system. Total of 13 bacterial strains; 5 gram-positive cocci, 7 gram-negative bacilli and Candida parapsilosis which were isolated mainly from blood cultures were used as the test strains. BACTEC 92F, 93F and BacT/ALERT FA, FN bottles were used as the blood culture bottles. All the bottles inoculated with the test strains were incubated and evaluated immediately after standing at room temperature for 24, 42, 48, 54 or 72 hours, using the respective automated blood culture systems...
November 2004: Kansenshōgaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
https://www.readbyqxmd.com/read/15614459/can-bact-alert-fa-and-fn-blood-culture-bottles-increase-the-recovery-of-microorganisms-in-the-clinical-laboratory
#20
Takashi Saito, Yoshitsugu Iinuma, Shunji Takakura, Naoko Fujihara, Toyoichiro Kudo, Satoshi Ichiyama
To evaluate whether the use of BacT/Alert FA and FN blood culture bottles increases the yield of microorganisms, we performed a before-after study. BacT/Alert standard aerobic (AE) and anaerobic (AN) bottles were used from January 1999 to May 2001 (before period). FA and FN bottles were used from May 2001 to March 2003 (after period). A total of 7796 AE, 7807 AN, 4798 FA, and 4787 FN bottles were processed. There were 742 (9.5%) AE-, 598 (7.7%) AN-, 521 (10.7%) FA-, and 396 (8.3%) FN-positive bottles. From these positive bottles 776, 631, 585, and 487 microorganisms were isolated, respectively...
December 2004: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
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