keyword
MENU ▼
Read by QxMD icon Read
search

Feature engineering

keyword
https://www.readbyqxmd.com/read/27909339/cancer-cell-motility-lessons-from-migration-in-confined-spaces
#1
Colin D Paul, Panagiotis Mistriotis, Konstantinos Konstantopoulos
Time-lapse, deep-tissue imaging made possible by advances in intravital microscopy has demonstrated the importance of tumour cell migration through confining tracks in vivo. These tracks may either be endogenous features of tissues or be created by tumour or tumour-associated cells. Importantly, migration mechanisms through confining microenvironments are not predicted by 2D migration assays. Engineered in vitro models have been used to delineate the mechanisms of cell motility through confining spaces encountered in vivo...
December 2, 2016: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/27909022/development-and-evaluation-of-the-tigriopus-course-based-undergraduate-research-experience-impacts-on-students-content-knowledge-attitudes-and-motivation-in-a-majors-introductory-biology-course
#2
Jeffrey T Olimpo, Ginger R Fisher, Sue Ellen DeChenne-Peters
Within the past decade, course-based undergraduate research experiences (CUREs) have emerged as a viable mechanism to enhance novices' development of scientific reasoning and process skills in the science, technology, engineering, and mathematics disciplines. Recent evidence within the bioeducation literature suggests that student engagement in such experiences not only increases their appreciation for and interest in scientific research but also enhances their ability to "think like a scientist." Despite these critical outcomes, few studies have objectively explored CURE versus non-CURE students' development of content knowledge, attitudes, and motivation in the discipline, particularly among nonvolunteer samples...
2016: CBE Life Sciences Education
https://www.readbyqxmd.com/read/27908716/self-healing-gelatin-ionogels
#3
REVIEW
Anshu Sharma, Kamla Rawat, Pratima R Solanki, H B Bohidar
We demonstrate room temperature (20°C) self-healing, and substantial recovery (68-96%) of gel rigidity of gelatin, a polypeptide, ionogels (made in 1-ethyl-3-methylimidazolium chloride ionic liquid (IL)solutions via thermal treatment, IL≤5% (w/v)) after they were cut using a surgical blade. The recovery process did not require any stimuli, and the complete healing under ambient condition required about 10hrs.The self-healing owed its origin to the reformation of network structures via imidazolium ion mediated charge quenching of deprotonated residues, and hydrophobic interaction between neighbouring alkyl tails of IL molecules...
November 28, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27907145/training-to-improve-precision-and-accuracy-in-the-measurement-of-fiber-morphology
#4
Nathan A Hotaling, Jun Jeon, Mary Beth Wade, Derek Luong, Xavier-Lewis Palmer, Kapil Bharti, Carl G Simon
An estimated $7.1 billion dollars a year is spent due to irreproducibility in pre-clinical data from errors in data analysis and reporting. Therefore, developing tools to improve measurement comparability is paramount. Recently, an open source tool, DiameterJ, has been deployed for the automated analysis of scanning electron micrographs of fibrous scaffolds designed for tissue engineering applications. DiameterJ performs hundreds to thousands of scaffold fiber diameter measurements from a single micrograph within a few seconds, along with a variety of other scaffold morphological features, which enables a more rigorous and thorough assessment of scaffold properties...
2016: PloS One
https://www.readbyqxmd.com/read/27905529/efficient-targeted-mutagenesis-of-rice-and-tobacco-genomes-using-cpf1-from-francisella-novicida
#5
Akira Endo, Mikami Masafumi, Hidetaka Kaya, Seiichi Toki
CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a newly characterized RNA-guided endonuclease that has two distinct features as compared to Cas9. First, Cpf1 utilizes a thymidine-rich protospacer adjacent motif (PAM) while Cas9 prefers a guanidine-rich PAM. Cpf1 could be used as a sequence-specific nuclease to target AT-rich regions of a genome that Cas9 had difficulty accessing. Second, Cpf1 generates DNA ends with a 5' overhang, whereas Cas9 creates blunt DNA ends after cleavage...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27899671/repeatsdb-2-0-improved-annotation-classification-search-and-visualization-of-repeat-protein-structures
#6
Lisanna Paladin, Layla Hirsh, Damiano Piovesan, Miguel A Andrade-Navarro, Andrey V Kajava, Silvio C E Tosatto
RepeatsDB 2.0 (URL: http://repeatsdb.bio.unipd.it/) is an update of the database of annotated tandem repeat protein structures. Repeat proteins are a widespread class of non-globular proteins carrying heterogeneous functions involved in several diseases. Here we provide a new version of RepeatsDB with an improved classification schema including high quality annotations for ∼5400 protein structures. RepeatsDB 2.0 features information on start and end positions for the repeat regions and units for all entries...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27899571/engineering-robust-and-tunable-spatial-structures-with-synthetic-gene-circuits
#7
Wentao Kong, Andrew E Blanchard, Chen Liao, Ting Lu
Controllable spatial patterning is a major goal for the engineering of biological systems. Recently, synthetic gene circuits have become promising tools to achieve the goal; however, they need to possess both functional robustness and tunability in order to facilitate future applications. Here we show that, by harnessing the dual signaling and antibiotic features of nisin, simple synthetic circuits can direct Lactococcus lactis populations to form programmed spatial band-pass structures that do not require fine-tuning and are robust against environmental and cellular context perturbations...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27896971/rabix-an-open-source-workflow-executor-supporting-recomputability-and-interoperability-of-workflow-descriptions
#8
Gaurav Kaushik, Sinisa Ivkovic, Janko Simonovic, Nebojsa Tijanic, Brandi Davis-Dusenbery, Deniz Kural
As biomedical data has become increasingly easy to generate in large quantities, the methods used to analyze it have proliferated rapidly. Reproducible and reusable methods are required to learn from large volumes of data reliably. To address this issue, numerous groups have developed workflow specifications or execution engines, which provide a framework with which to perform a sequence of analyses. One such specification is the Common Workflow Language, an emerging standard which provides a robust and flexible framework for describing data analysis tools and workflows...
2016: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/27896935/sandcastle-worm-inspired-blood-resistant-bone-graft-binder-using-a-sticky-mussel-protein-for-augmented-in-vivo-bone-regeneration
#9
Hyo Jeong Kim, Bong-Hyuk Choi, Sang Ho Jun, Hyung Joon Cha
Xenogenic bone substitutes are commonly used during orthopedic reconstructive procedures to assist bone regeneration. However, huge amounts of blood accompanied with massive bone loss usually increase the difficulty of placing the xenograft into the bony defect. Additionally, the lack of an organic matrix leads to a decrease in the mechanical strength of the bone-grafted site. For effective bone grafting, this study aims at developing a mussel adhesion-employed bone graft binder with great blood-resistance and enhanced mechanical properties...
November 29, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#10
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27893794/single-amino-acid-repeats-in-the-proteome-world-structural-functional-and-evolutionary-insights
#11
Amitha Sampath Kumar, Divya Tej Sowpati, Rakesh K Mishra
Microsatellites or simple sequence repeats (SSR) are abundant, highly diverse stretches of short DNA repeats present in all genomes. Tandem mono/tri/hexanucleotide repeats in the coding regions contribute to single amino acids repeats (SAARs) in the proteome. While SSRs in the coding region always result in amino acid repeats, a majority of SAARs arise due to a combination of various codons representing the same amino acid and not as a consequence of SSR events. Certain amino acids are abundant in repeat regions indicating a positive selection pressure behind the accumulation of SAARs...
2016: PloS One
https://www.readbyqxmd.com/read/27888602/a-glycovariant-of-human-cd44-is-characteristically-expressed-on-human-mesenchymal-stem-cells
#12
Gisela Pachón-Peña, Conor Donnelly, Catalina Ruiz-Cañada, Adam Katz, Sonia Fernández-Veledo, Joan Vendrell, Robert Sackstein
The clinical effectiveness of systemically-administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase- VI (FTVI) to enforce sLe(X) decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin...
November 26, 2016: Stem Cells
https://www.readbyqxmd.com/read/27888100/harnessing-wharton-s-jelly-stem-cell-differentiation-into-bone-like-nodule-on-calcium-phosphate-substrate-without-osteoinductive-factors
#13
S Mechiche Alami, H Rammal, C Boulagnon-Rombi, F Velard, F Lazar, R Drevet, D Laurent Maquin, S C Gangloff, J Hemmerlé, J C Voegel, G Francius, P Schaaf, F Boulmedais, H Kerdjoudj
An important aim of bone regenerative medicine is to design biomaterials with controlled chemical and topographical features to guide stem cell fate towards osteoblasts without addition of specific osteogenic factors. Herein, we find that sprayed bioactive and biocompatible calcium phosphate substrates (CaP) with controlled topography induce, in a well-orchestrated manner, Wharton's jelly stem cells (WJ-SCs) differentiation into osteoblastic lineage without any osteogenic supplements. The resulting WJ-SCs commitment exhibits features of native bone, through the formation of three-dimensional bone-like nodule with osteocyte-like cells embedded into a mineralized type I collagen...
November 22, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27886208/flexible-controls-of-scattering-clouds-using-coding-metasurfaces
#14
Shuo Liu, Tie Jun Cui
Metamaterials or metasurfaces have been designed to precisely manipulate the scattering at every angle. Here, we propose to control the probability of random scattering appearing in the desired range of angles, which is defined in this letter as scattering cloud. We present a controllable random metasurface by simply adding a random coding sequence to gradient coding sequence. It is shown that the direction and size of the scattering cloud can be arbitrarily engineered. We demonstrate the exotic behavior of the scattering cloud by making an analogy to the electron cloud in quantum mechanics...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27885768/gram-scale-synthesis-of-chiral-cyclopropane-containing-drugs-and-drug-precursors-with-engineered-myoglobin-catalysts-featuring-complementary-stereoselectivity
#15
Priyanka Bajaj, Gopeekrishnan Sreenilayam, Vikas Tyagi, Rudi Fasan
Engineered hemoproteins have recently emerged as promising systems for promoting asymmetric cyclopropanations, but variants featuring predictable, complementary stereoselectivity in these reactions have remained elusive. In this study, a rationally driven strategy was implemented and applied to engineer myoglobin variants capable of providing access to 1-carboxy-2-aryl-cyclopropanes with high trans-(1R,2R) selectivity and catalytic activity. The stereoselectivity of these cyclopropanation biocatalysts complements that of trans-(1S,2S)-selective variants developed here and previously...
November 25, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27884775/tuning-acoustic-and-mechanical-properties-of-materials-for-ultrasound-phantoms-and-smart-substrates-for-cell-cultures
#16
A Cafarelli, A Verbeni, A Poliziani, P Dario, A Menciassi, L Ricotti
: Materials with tailored acoustic properties are of great interest for both the development of tissue-mimicking phantoms for ultrasound tests and smart scaffolds for ultrasound mediated tissue engineering and regenerative medicine. In this study, we assessed the acoustic properties (speed of sound, acoustic impedance and attenuation coefficient) of three different materials (agarose, polyacrylamide and polydimethylsiloxane) at different concentrations or cross-linking levels and doped with different concentrations of barium titanate ceramic nanoparticles...
November 21, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27884105/structural-features-of-dna-that-determine-rna-polymerase-ii-core-promoter
#17
Irina A Il'icheva, Mingian V Khodikov, Maria S Poptsova, Dmitry Yu Nechipurenko, Yury D Nechipurenko, Sergei L Grokhovsky
BACKGROUND: The general structure and action of all eukaryotic and archaeal RNA polymerases machinery have an astonishing similarity despite the diversity of core promoter sequences in different species. The goal of our work is to find common characteristics of DNA region that define it as a promoter for the RNA polymerase II (Pol II). RESULTS: The profiles of a large number of physical and structural characteristics, averaged over representative sets of the Pol II minimal core promoters of the evolutionary divergent species from animals, plants and unicellular fungi were analysed...
November 25, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27883932/biological-response-of-human-suture-mesenchymal-cells-to-titania-nanotube-based-implants-for-advanced-craniosynostosis-therapy
#18
Manpreet Bariana, Prem Dwivedi, Sarbin Ranjitkar, John A Kaidonis, Dusan Losic, Peter J Anderson
Titania nanotubes (TNTs) engineered on titanium (Ti) surfaces (i.e. TNT/Ti) and loaded with specific drugs have been recognised as a promising solution for localised therapeutic delivery to address several medical problems not feasible with conventional drug administration. We propose the use of TNT/Ti protein-releasing implants to treat paediatric craniofacial abnormality in craniosynostosis caused by premature fusion of cranial sutures. In this study, we have analysed the biological response of human suture mesenchymal cells (SMCs), extracted from two different patients undergoing craniofacial reconstruction surgery, at the TNT/Ti implant surface...
November 17, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27882503/proving-that-china-has-a-profession-of-engineering-a-case-study-in-operationalizing-a-concept-across-a-cultural-divide
#19
Michael Davis, Hengli Zhang
This article assumes that a profession is a number of individuals in the same occupation voluntarily organized to earn a living by openly serving a moral ideal in a morally-permissible way (a discipline) beyond what law, market, morality, and public opinion would otherwise require. Our question is whether the concept of profession (so defined) may have a far wider range than the term, so that, for example, pointing out that a certain language lacks a word for "profession" in our sense, is not enough to show that those who speak the language also lack the concept...
November 23, 2016: Science and Engineering Ethics
https://www.readbyqxmd.com/read/27881648/modeling-severe-fever-with-thrombocytopenia-syndrome-virus-infection-in-golden-syrian-hamsters-importance-of-stat2-in-preventing-disease-and-effective-treatment-with-favipiravir
#20
Brian B Gowen, Jonna B Westover, Jinxin Miao, Arnaud J Van Wettere, Johanna D Rigas, Brady T Hickerson, Kie-Hoon Jung, Rong Li, Bettina L Conrad, Skot Nielson, Yousuke Furuta, Zhongde Wang
: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick-borne disease endemic in parts of Asia. The etiologic agent, SFTS virus (SFTSV; Bunyaviridae family, Phlebovirus genus) has caused significant morbidity and mortality in China, Korea, and Japan, with key features of disease being intense fever, thrombocytopenia, and leukopenia. Case fatality rates are estimated in the 30% range and no antivirals or vaccines are approved for use for treatment and prevention of SFTS...
November 23, 2016: Journal of Virology
keyword
keyword
104971
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"