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Salivary diagnostics

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https://www.readbyqxmd.com/read/27925697/prevalence-of-sj%C3%A3-gren-s-syndrome-in-brazilian-patients-infected-with-human-t-cell-lymphotropic-virus
#1
Daniela Assis do Vale, Jorge Casseb, Augusto César Penalva de Oliveira, Ivo Bussoloti Filho, Suzana Cantanhede Orsini Machado de Sousa, Karem López Ortega
BACKGROUND: Human T-lymphotropic virus type I (HTLV-I) is known to be associated with neoplastic and neurodegenerative changes, and it is believed to be associated with various systemic inflammatory diseases, including Sjögren's syndrome (SS). Although HTLV-I infection is endemic in Brazil, there is no information regarding the association between HTLV-I infection and SS in the Brazilian population. The objective of this study was to determine the prevalence of SS in HTLV-I-infected individuals and the prevalence of HTLV-I-infection in individuals diagnosed with SS...
December 7, 2016: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/27924586/rna-sequencing-analysis-of-salivary-extracellular-rna
#2
Blanca Majem, Feng Li, Jie Sun, David T W Wong
Salivary biomarkers for disease detection, diagnostic and prognostic assessments have become increasingly well established in recent years. In this chapter we explain the current leading technology that has been used to characterize salivary non-coding RNAs (ncRNAs) from the extracellular RNA (exRNA) fraction: HiSeq from Illumina® platform for RNA sequencing. Therefore, the chapter is divided into two main sections regarding the type of the library constructed (small and long ncRNA libraries), from saliva collection, RNA extraction and quantification to cDNA library generation and corresponding QCs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27924585/salivary-diagnostics-using-purified-nucleic-acids
#3
Paul D Slowey
Saliva is an easily accessible fluid that has led to increasing interest in the development of salivary diagnostics. This chapter describes some of the newer tools and procedures for collection, stabilization, and storage of oral fluid matrices that aid in the successful use of saliva as a test specimen. This chapter focuses particularly on nucleic acid components for downstream molecular diagnostic (MDx) testing, since this is probably the area where saliva is likely to have the greatest impact in improving healthcare for the general population...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27917674/-giant-sialolithiasis-diagnosis-and-management-case-reports
#4
Bálint Tordai, Nóra Stáczer, László Vajta, József Szalma
Sialolithiasis is one of the most frequent form of calcifications in the maxillofacial area. 0.45% of the population is affected by symptoms caused by salivary calculi, though the estimated frequency including asymptomatic form may exceed 1% in adult population. Radiographs presenting a large portion of the maxillofacial region (panoramic radiography, computed tomography) could detect salivary calculi with high accuracy. The size of the sialoliths is usually less than 10 mm in diameter. Salivary calculi larger than 15 mm (considering the largest diameter) are classified as giant sialoliths and most of them are located in the submandibular gland or in its duct...
December 2016: Orvosi Hetilap
https://www.readbyqxmd.com/read/27903834/saliva-diagnostics-current-views-and-directions
#5
Karolina Elżbieta Kaczor-Urbanowicz, Carmen Martin Carreras-Presas, Katri Aro, Michael Tu, Franklin Garcia-Godoy, David Tw Wong
In this review, we provide an update on the current and future applications of saliva for diagnostic purposes. There are many advantages of using saliva as a biofluid. Its collection is fast, easy, inexpensive, and non-invasive. In addition, saliva, as a "mirror of the body," can reflect the physiological and pathological state of the body. Therefore, it serves as a diagnostic and monitoring tool in many fields of science such as medicine, dentistry, and pharmacotherapy. Introduced in 2008, the term "Salivaomics" aimed to highlight the rapid development of knowledge about various "omics" constituents of saliva, including: proteome, transcriptome, micro-RNA, metabolome, and microbiome...
November 29, 2016: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27900251/salivary-biomarkers-in-oral-squamous-cell-carcinoma-an-insight
#6
REVIEW
T Radhika, Nadeem Jeddy, S Nithya, R M Muthumeenakshi
Oral cancer refers to the malignancies that occur in the oral cavity, lip and pharynx with 90% of oral cancers being squamous cell carcinomas (OSCC). OSCC has the highest mortality ratio compared to other carcinomas. Although oral cavity is easily accessible, most oral cancers are detected at a later stage leading to lower survival rates. Early detection of OSCC is a key factor in improving the prognosis and survival rate of the patient. Rapid advancement in the field of diagnosis has enabled early diagnosis of many potentially malignant conditions even before its clinical manifestations...
November 2016: Journal of Oral Biology and Craniofacial Research
https://www.readbyqxmd.com/read/27900241/changes-in-the-salivary-oxidative-status-in-individuals-with-temporomandibular-disorders-and-pain
#7
Claudyane de Almeida, José Miguel Amenábar
Temporomandibular joint disorders are quite common among the general public. Free radicals may play a role in the pathogenesis of joint diseases, and the oxidative stress is an important aspect in the mechanism of TMDs. The use of new biotechnologies has enabled the use of saliva as a diagnostic method. This is the first paper that aims to investigate changes in the oxidative status, through saliva analysis, in individuals who suffer from temporomandibular disorder and pain. Sixty individuals, 54 women and 06 men, aged between 10 and 60, participated in this research...
November 2016: Journal of Oral Biology and Craniofacial Research
https://www.readbyqxmd.com/read/27893660/salivary-lactate-dehydrogenase-and-aminotransferases-in-diabetic-patients
#8
Barbara Malicka, Katarzyna Skoskiewicz-Malinowska, Urszula Kaczmarek
Diabetes mellitus (DM) is a group of metabolic diseases resulting from impaired insulin secretion and/or action. DM is characterized by hyperglycemia that can lead to the dysfunction or damage of organs, including the salivary glands.The aim of this study was to compare the levels of salivary lactate dehydrogenase (LDH), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in diabetic patients.The study was approved by the Bioethics Committee of Wroclaw Medical University (Poland). The study comprised 90 adults of both sexes, aged 21 to 57 years...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27885225/the-physical-signs-of-impending-dehydration-among-elderly-people-in-nursing-homes-the-association-with-axillary-skin-temperature-humidity-intraoral-moisture-content-and-salivary-components
#9
Mayumi Okuyama, Masumi Nishida
PURPOSE: The aim of the present study was to examine the association between impending dehydration among elderly people in nursing homes and physical signs, including the axillary skin temperature, humidity, intraoral moisture content, and salivary components. METHODS: The study included 78 elderly individuals who required long-term care in a nursing home (11 men and 67 women; average age, 86.6±7.3 years). The elderly subjects were classified in two groups according to their serum osmolality levels: those with levels between the upper limit reference value (292 mOsm/kg H2O) and the diagnostic reference value of dehydration (300 mOsm/kg H2O) were classified into the boundary zone group and those with levels of <292 mOsm/kg H2O were classified into the normal range group...
2016: Nihon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics
https://www.readbyqxmd.com/read/27882318/sialendoscopy-and-combined-minimally-invasive-treatment-for-large-parotid-stones
#10
Jan Rotnágl, Šárka Zavázalová, Olexii Vorobiov, Jaromír Astl
Sialendoscopy (SE) represents nowadays one of the standard diagnostic and therapeutic procedures in the treatment of major salivary glands lithiasis. We know from experience that it is successful only in small percentage of patients, when used in monotherapy. However, it represents an indispensable part of all of the combined minimally invasive gland-preserving treatment techniques, the success rate of which is around 90%. In this work, we focused on the role of sialendoscopy in the treatment of patients with larger inflamed fixed stones in glandula parotis...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27880945/immunohistochemical-staining-of-histological-fragments-derived-from-salivary-gland-tumour-fine-needle-biopsy-aspirates
#11
Marie Lundberg, Berent Munsterhjelm, Antti Mäkitie, Ilmo Leivo
OBJECTIVES: The aim of this study was to describe a method for analysing histological fragments derived from fine- needle aspirate biopsy (FNAB) of salivary gland tumours (SGTs), and to evaluate the use of immunohistochemistry (IHC) on them. STUDY DESIGN: We reviewed all 509 FNAB pathology reports taken from SGTs at Helsinki University Hospital, Finland, between 1999 and 2009. In 51% of the cases (n = 209) "histo-fragments" had been obtained and 31 had been further analysed by IHC...
November 24, 2016: Acta Cytologica
https://www.readbyqxmd.com/read/27873102/fine-needle-cytology-in-the-follow-up-of-breast-carcinoma
#12
Anna Maria Cascone, Maria Siano, Basilio Angrisani, Rosa Maria Di Crescenzo, Alessandro Vatrella, Carmine Selleri, Francesco Salzano, Pio Zeppa
The postoperative follow-up strategies for breast carcinoma (BC) utilize different procedures; the aim of this study was to investigate the role of fine-needle cytology (FNC) in the follow-up of BC patients. Two hundred sixty-six FNC samples from 190 BC patients have been reviewed. The target anatomical sites were 190 breast including 155 ipsilateral and 145 contralateral breast lesions and 76 extra-mammary nodules. Extra-mammary lesions included lymph nodes, thyroidal nodules, soft tissue lesions, (subcutaneous and sub-scars), salivary glands and deep located masses...
November 21, 2016: Aging Clinical and Experimental Research
https://www.readbyqxmd.com/read/27872417/virtual-touch-quantification-of-the-salivary-glands-for-diagnosis-of-primary-sj%C3%A3-gren-syndrome
#13
Shaoqi Chen, Yukai Wang, Shaoxing Chen, Qiulin Wu, Shigao Chen
OBJECTIVES: To investigate the value of salivary gland stiffness measured by Virtual Touch quantification (VTQ; Siemens Medical Solutions, Erlangen, Germany) for assessment of primary Sjögren syndrome. METHODS: Fifty-four patients with primary Sjögren syndrome, 35 patients without primary Sjögren syndrome (patients with dry mouth and dry eye symptoms), and 52 healthy control volunteers were included in this study. Patients with primary Sjögren syndrome were classified as early or advanced stage by labial gland biopsies...
December 2016: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/27872416/initial-experience-with-ultrasound-elastography-for-diagnosis-of-major-salivary-gland-lesions
#14
Hang Zhou, Xian-Li Zhou, Hui-Xiong Xu, Ya-Ping He, Xiao-Wan Bo, Xiao-Long Li, Bo-Ji Liu, Dan-Dan Li, Dan Wang
OBJECTIVES: The aim of this study was to evaluate the usefulness of ultrasound elastography, including conventional strain elastography, acoustic radiation force impulse (ARFI)-induced strain elastography, and point shear wave elastography (SWE) for diagnosis of major salivary gland lesions. METHODS: Forty major salivary gland lesions underwent conventional sonography, conventional strain elastography, ARFI strain elastography, and point SWE before surgery or biopsy...
December 2016: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
https://www.readbyqxmd.com/read/27862926/emerging-technologies-for-salivaomics-in-cancer-detection
#15
REVIEW
Karolina Elżbieta Kaczor-Urbanowicz, Carmen Martín Carreras-Presas, Tadeusz Kaczor, Michael Tu, Fang Wei, Franklin Garcia-Godoy, David T W Wong
Salivary diagnostics has great potential to be used in the early detection and prevention of many cancerous diseases. If implemented with rigour and efficiency, it can result in improving patient survival times and achieving earlier diagnosis of disease. Recently, extraordinary efforts have been taken to develop non-invasive technologies that can be applied without complicated and expensive procedures. Saliva is a biofluid that has demonstrated excellent properties and can be used as a diagnostic fluid, since many of the biomarkers suggested for cancers can also be found in whole saliva, apart from blood or other body fluids...
November 13, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27843342/the-danish-head-and-neck-cancer-database
#16
REVIEW
Jens Overgaard, Aleksandar Jovanovic, Christian Godballe, Jesper Grau Eriksen
AIM OF THE DATABASE: The Danish Head and Neck Cancer database is a nationwide clinical quality database that contains prospective data collected since the early 1960s. The overall aim of this study was to describe the outcome of the national strategy for multidisciplinary treatment of head and neck cancer in Denmark and to create a basis for clinical trials. STUDY POPULATION: The study population consisted of all Danish patients referred for treatment of squamous cell carcinoma of the larynx, pharynx, oral cavity, or neck nodes from unknown primary or any histopathological type (except lymphoma) of cancer in the nasal sinuses, salivary glands, or thyroid gland (corresponding to the International Classification of Diseases, tenth revision, classifications C...
2016: Clinical Epidemiology
https://www.readbyqxmd.com/read/27832115/the-potential-use-of-salivary-mirnas-as-promising-biomarkers-for-detection-of-cancer-a-meta-analysis
#17
Yuanjie Ding, Qing Ma, Fen Liu, Lei Zhao, Wenqiang Wei
BACKGROUND: Accumulating evidence has demonstrated that microRNAs (miRNAs) could serve as promising molecular biomarkers for cancer detection. This study aims to systematically assess the diagnostic performance of salivary miRNAs in detection of cancer through a comprehensive meta-analysis. METHODS: Eligible studies were identified using PubMed and other computerized databases up to October 31, 2015, supplemented by a manual search of references from retrieved articles...
2016: PloS One
https://www.readbyqxmd.com/read/27829772/mucocele-a-diagnostic-dilemma
#18
Maruthamuthu Karthikeyan, Alex K Varghese, Govindarajan Vasupradha, Jayapal Dinakaran
Oral mucocele is the most common benign lesion of minor salivary gland caused due to any form of mechanical trauma to the excretory duct of the gland. It is of two types - mucous extravasation phenomenon and mucous retention type. Extravasation type is more common. Here, we report a case of a 65-year-old male patient with a complaint of painful swelling in the lower lip for 1 month with a history of trauma in the lower lip. It was clinically diagnosed as traumatic fibroma, and an excisional biopsy was done...
October 2016: Journal of Pharmacy & Bioallied Sciences
https://www.readbyqxmd.com/read/27812776/critical-review-of-igg4-related-hypophysitis
#19
Junpei Shikuma, Kenshi Kan, Rokuro Ito, Kazuo Hara, Hiroyuki Sakai, Takashi Miwa, Akira Kanazawa, Masato Odawara
PURPOSE: IgG4-related hypophysitis is a rare disease, with only 34 cases published in English (2015). Available short reviews may not present complete details of IgG4-related hypophysitis. We aimed to survey case reports of IgG4-related hypophysitis, including abstracts of scientific meetings, in English and Japanese. METHODS: We searched for information about IgG4-related hypophysitis in PubMed and Igakuchuozasshi (Japan Medical Abstracts Society). Among 104 case reports found, we reviewed 84 fulfilling Leporati's diagnostic criteria...
November 3, 2016: Pituitary
https://www.readbyqxmd.com/read/27806890/the-added-value-of-pretreatment-dw-mri-in-characterization-of-salivary-glands-pathologies
#20
Peter Milad, Marwa Elbegiermy, Tamer Shokry, Heba Mahmoud, Ihab Kamal, Mohamed Shehata Taha, Nermeen Keriakos
OBJECTIVE: To evaluate the added value of diffusion weighted magnetic resonance imaging (DW-MRI) in characterization of salivary gland lesions. STUDY DESIGN: A prospective study was carried out between January 2013, and March 2015. METHODS: The study included 46 patients. The consultant radiologist, who reviewed the scans to comment on the apparent diffusion coefficient (ADC) value and ADC histogram was blind to the suspected pathology. Radiological findings were then compared to clinical and histological findings...
September 7, 2016: American Journal of Otolaryngology
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