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https://www.readbyqxmd.com/read/29781670/genetic-characterisation-of-virulence-genes-associated-with-adherence-invasion-and-cytotoxicity-in-campylobacter-spp-isolated-from-commercial-chickens-and-human-clinical-cases
#1
Samantha Reddy, Oliver T Zishiri
Virulence-associated genes have been recognised and detected in Campylobacter species. The majority of them have been proven to be associated with pathogenicity. This study aimed to detect the presence of virulence genes associated with pathogenicity and responsible for invasion, expression of adherence, colonisation and production of the cytolethal distending toxin (cdt) in Campylobacter jejuni and Campylobacter coli. Commercial chicken faecal samples were randomly sampled from chicken farms within the Durban metropolitan area in South Africa...
February 15, 2018: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/29681602/-dose-distribution-optimization-in-combined-intracavitary-and-interstitial-brachytherapy-for-cervical-cancer
#2
Daisuke Ozaki, Makoto Miyazawa, Youji Habano, Motohiro Kawashima, Yoshihiko Hoshino, Takayuki Sutou
PURPOSE: Hybrid inverse treatment planning optimization (HIPO) is a new optimization tool for brachytherapy. We verified its utility using treatment plans for combined intracavitary and interstitial brachytherapy in cervical cancer. MATERIALS & METHODS: We compared the manually optimized plan and the plan optimized using HIPO. The plan using HIPO was optimized with three different methods: needle only, tandem and needle, and all applicators. The dose volume histogram (DVH) parameters such as D90 of high risk clinical target volume (HR-CTV) and D2cc of OAR (rectum, sigmoid colon and bladder) were used to evaluate each treatment plan...
2018: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/29377719/prevalence-and-antibiotic-resistance-profiles-of-campylobacter-jejuni-isolated-from-poultry-meat-and-related-samples-at-retail-shops-in-northern-india
#3
Javed Ahamad Khan, Ram Swaroop Rathore, Hussein Hasan Abulreesh, Faizan Abul Qais, Iqbal Ahmad
Campylobacteriosis is the common gastrointestinal disease worldwide. However, in many parts of the world, including India, the impact of campylobacteriosis is less commonly investigated. This study aimed to determine the prevalence and antibiotic susceptibility profiles of Campylobacter jejuni in raw poultry meat and poultry-related samples at retail shops in a region of Northern India. A total of 400 samples of chicken meat (150), chicken intestine (150), feathers (50), and chopping boards and knives (50) samples were screened for the presence of C...
April 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/29356597/rapid-and-specific-methods-to-differentiate-foodborne-pathogens-campylobacter-jejuni-campylobacter-coli-and-the-new-species-causing-spotty-liver-disease-in-chickens-campylobacter-hepaticus
#4
Thi Thu Hao Van, Arif Anwar, Peter C Scott, Robert J Moore
Campylobacter jejuni and Campylobacter coli play a major role in bacteria-related foodborne illness in humans. Recently, a newly identified species, Campylobacter hepaticus, was shown to be the causative agent of spotty liver disease in chickens. The pathogenic potential of C. hepaticus in humans is unknown. This new species contains genes usually used to detect C. jejuni and C. coli in DNA-based detection methods, such as the hippuricase (hipO) gene and the glyA (serine hydroxymethyltransferase) gene, with a high degree of similarity...
January 22, 2018: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28921538/a-fast-inverse-treatment-planning-strategy-facilitating-optimized-catheter-selection-in-image-guided-high-dose-rate-interstitial-gynecologic-brachytherapy
#5
Christian V Guthier, Antonio L Damato, Juergen W Hesser, Akila N Viswanathan, Robert A Cormack
PURPOSE: Interstitial high-dose rate (HDR) brachytherapy is an important therapeutic strategy for the treatment of locally advanced gynecologic (GYN) cancers. The outcome of this therapy is determined by the quality of dose distribution achieved. This paper focuses on a novel yet simple heuristic for catheter selection for GYN HDR brachytherapy and their comparison against state of the art optimization strategies. The proposed technique is intended to act as a decision-supporting tool to select a favorable needle configuration...
December 2017: Medical Physics
https://www.readbyqxmd.com/read/28581253/severe-hypoglycaemia-among-patients-with-type-2-diabetes-requiring-emergency-hospital-admission-the-hypoglycaemia-in-portugal-observational-study-emergency-room-hipos-er
#6
João Conceição, Jorge Dores, Francisco Araújo, Pedro A Laires, Richard D Carr, Kimberly Brodovicz, Larry Radican, Ana M Nogueira
AIMS: To analyse the prevalence of severe hypoglycaemia in patients with type 2 diabetes (T2DM) treated with antihyperglycaemic agents (AHA) and requiring emergency room (ER) assistance, and to analyse the prevalence according to type of AHA therapy. METHODS: The present study, the Hypoglycaemia In Portugal Observational Study-Emergency Room (HIPOS-ER), was a cross-sectional, observational, multicentre, nationwide study, with specific hypoglycaemia source data collection...
January 2018: Diabetes, Obesity & Metabolism
https://www.readbyqxmd.com/read/28364369/predicting-productivity-losses-from-health-related-quality-of-life-using-patient-data
#7
Clara Mukuria, Donna Rowen, Mónica Hernández-Alava, Simon Dixon, Roberta Ara
OBJECTIVE: This paper estimates productivity loss using the health of the patient in order to allow indirect estimation of these costs for inclusion in economic evaluation. METHODS: Data from two surveys of inpatients [Health outcomes data repository (HODaR) sample (n = 42,442) and health improvement and patient outcomes (HIPO) sample (n = 6046)] were used. The number of days off paid employment or normal activities (excluding paid employment) was modelled using the health of the patients measured by the EQ-5D, international classification of diseases (ICD) chapters, and other health and sociodemographic data...
October 2017: Applied Health Economics and Health Policy
https://www.readbyqxmd.com/read/28160162/isolation-identification-and-differentiation-of-campylobacter-spp-using-multiplex-pcr-assay-from-goats-in-khartoum-state-sudan
#8
Atif Elbrissi, Y A Sabeil, Khalda A Khalifa, Khalid Enan, Osama M Khair, A M El Hussein
The aim of this study was to identify and characterize thermophilic Campylobacter species in faecal samples from goats in Khartoum State, Sudan, by application of multiplex polymerase chain reaction. Campylobacteriosis is a zoonotic disease of global concern, and the organisms can be transmitted to human via food, water and through contact with farm animals and pets. There are five clinically related Campylobacter species: Campylobacter jejuni (C. jejuni). Campylobacter coli, Campylobacter lari, Campylobacter upsaliensis and Campylobacter fetus...
March 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/27847427/prevalence-of-campylobacter-jejuni-and-campylobacter-coli-in-raw-milk-and-some-dairy-products
#9
Mona A El-Zamkan, Karima G Abdel Hameed
AIM: This study was accomplished to test raw milk and certain dairy products sold in local markets of Qena, Egypt, for the presence of Campylobacter coli and Campylobacter jejuni. MATERIALS AND METHODS: A total of 150 samples of raw milk, kareish cheese, and yoghurt (50 samples each) were subjected first to enrichment in Bolton broth at 42°C for 2 days under a microaerobic condition, subsequently campylobacter blood free selective agar plates were cultured and incubated in the same condition of the broth...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27552785/multiplex-pcr-assay-for-identifi-cation-and-differentiation-of-campylobacter-jejuni-and-campylobacter-coli-isolates
#10
Maria R Pavlova, Elina G Dobreva, Katucha I Ivanova, Galina D Asseva, Ivan N Ivanov, Peter K Petrov, Valeri R Velev, Ivelina I Tomova, Maida M Tiholova, Todor V Kantardjiev
INTRODUCTION: Campylobacter spp. are important causative agents of gastrointestinal infections in humans. The most frequently isolated strains of this bacterial genus are Campylobacter jejuni and Campylobacter coli. To date, genetic methods for bacterial identification have not been used in Bulgaria. We optimized the multiplex PSR assay to identify Campylobacter spp. and differentiate C. jejuni from C. coli in clinical isolates. We also compared this method with the routinely used biochemical methods...
April 2016: Folia Medica
https://www.readbyqxmd.com/read/27200686/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-costs-and-health-care-resource-consumption-data
#11
P Laires, J Conceição, F Araújo, J Dores, C Silva, L Radican, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27200579/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-clinical-outcomes-in-admitted-patients
#12
J Conceição, J Dores, F Araújo, P Laires, R Carr, K Brodovicz, L Radican, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27200577/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-clinical-outcomes-in-the-emergency-room
#13
J Conceição, J Dores, F Araújo, P Laires, R Carr, K Brodovicz, L Radican, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27200576/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-outcomes-with-different-anti-hyperglycemic-agents
#14
J Conceição, P Laires, J Dores, F Araújo, R Carr, K Brodovicz, L Radican, V Vicente, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/26783018/comparison-of-antimicrobial-susceptibility-of-campylobacter-strains-isolated-from-food-samples-and-patients-with-diarrhea
#15
COMPARATIVE STUDY
Bita Bakhshi, Amin Naseri, Masoud Alebouyeh
BACKGROUND: Campylobacter infections may lead to serious conditions, including septicemia or other invasive forms of the disease, which require rapid and accurate laboratory diagnosis and subsequently appropriate antimicrobial therapy. The aim of this study was to compare the species distribution and antimicrobial susceptibility pattern of Campylobacter spp. strains isolated from patients and food samples. METHODS: Biochemical identification was performed on 15 clinical and 30 food isolates of Campylobacter recovered onto Brucella agar containing 5% sheep blood...
2016: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/26602171/comparison-of-antimicrobial-susceptibility-of-campylobacter-strains-isolated-from-food-samples-and-patients-with-diarrhea
#16
Bita Bakhshi, Amin Naseri, Masoud Alebouyeh
BACKGROUND: Campylobacter infections may lead to serious conditions, including septicemia or other invasive forms of the disease, which require rapid and accurate laboratory diagnosis and subsequently appropriate antimicrobial therapy. The aim of this study was to compare the species distribution and antimicrobial susceptibility pattern of Campylobacter spp. strains isolated from patients and food samples. METHODS: Biochemical identification was performed on 15 clinical and 30 food isolates of Campylobacter recovered onto Brucella agar containing 5% sheep blood...
November 25, 2015: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/26561276/comparison-of-dose-and-catheter-optimization-algorithms-in-prostate-high-dose-rate-brachytherapy
#17
COMPARATIVE STUDY
Eric Poulin, Nicolas Varfalvy, Sylviane Aubin, Luc Beaulieu
PURPOSE: The purpose of this work was to compare the hybrid inverse treatment planning optimization (HIPO), inverse dose-volume histogram-based optimization (DVHO), and fast simulated annealing stochastic algorithm (IPSA). The catheter optimization algorithm HIPO was also compared with the Centroidal Voronoi Tessellation (CVT) algorithm. METHODS AND MATERIALS: In this study, eight high-dose-rate prostate cases were randomly selected from an anonymized bank of patients...
January 2016: Brachytherapy
https://www.readbyqxmd.com/read/26504034/dosimetric-and-radiobiological-evaluation-of-hybrid-inverse-planning-and-optimization-for-cervical-cancer-brachytherapy
#18
Lucílio Dos S Matias, Tomas Palmqvist, Jeanette Wolke, Josef Nilsson, Catharina Beskow, Alexandre M Maphossa, Iuliana Toma-Dasu
AIM: To compare manual graphical optimization (GrO) with hybrid inverse planning optimization (HIPO) of cervical cancer brachytherapy treatment plans using physical and radiobiological tools. PATIENTS AND METHODS: Ten patients suffering from cervical cancer, treated with pulsed brachytherapy using GrO plans, were included in the study. For each patient, four different HIPO class solutions with different dose objectives to the target and constraints to the organs at risk (OAR) produced four optimized plans, that were each compared to the corresponding GrO plan...
November 2015: Anticancer Research
https://www.readbyqxmd.com/read/26394042/differentiation-of-campylobacter-jejuni-and-campylobacter-coli-using-multiplex-pcr-and-high-resolution-melt-curve-analysis
#19
Banya Banowary, Van Tuan Dang, Subir Sarker, Joanne H Connolly, Jeremy Chenu, Peter Groves, Michelle Ayton, Shane Raidal, Aruna Devi, Thiru Vanniasinkam, Seyed A Ghorashi
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolates. A segment of the hippuricase gene (hipO) of C. jejuni and putative aspartokinase (asp) gene of C. coli were amplified from 26 Campylobacter isolates and amplicons were subjected to HRM curve analysis...
2015: PloS One
https://www.readbyqxmd.com/read/26187833/real-time-pcr-detection-of-campylobacter-spp-a-comparison-to%C3%A2-classic-culturing-and-enrichment
#20
COMPARATIVE STUDY
P de Boer, H Rahaoui, R J Leer, R C Montijn, J M B M van der Vossen
The major disadvantage of the current gold standard for detection of the food pathogen Campylobacter, i.e. culturing, is the lengthy procedure. In this study we assessed the use of real-time PCR for detection of Campylobacter. To this end, 926 poultry samples, taken from transport containers and broiler caeca in The Netherlands in 2007, were subjected to three different real-time PCR detection methods: one targeting the Campylobacter jejuni hipO gene, one targeting the Campylobacter coli glyA gene, and one generically targeting Campylobacter spp...
October 2015: Food Microbiology
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