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https://www.readbyqxmd.com/read/27847427/prevalence-of-campylobacter-jejuni-and-campylobacter-coli-in-raw-milk-and-some-dairy-products
#1
Mona A El-Zamkan, Karima G Abdel Hameed
AIM: This study was accomplished to test raw milk and certain dairy products sold in local markets of Qena, Egypt, for the presence of Campylobacter coli and Campylobacter jejuni. MATERIALS AND METHODS: A total of 150 samples of raw milk, kareish cheese, and yoghurt (50 samples each) were subjected first to enrichment in Bolton broth at 42°C for 2 days under a microaerobic condition, subsequently campylobacter blood free selective agar plates were cultured and incubated in the same condition of the broth...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27552785/multiplex-pcr-assay-for-identifi-cation-and-differentiation-of-campylobacter-jejuni-and-campylobacter-coli-isolates
#2
Maria R Pavlova, Elina G Dobreva, Katucha I Ivanova, Galina D Asseva, Ivan N Ivanov, Peter K Petrov, Valeri R Velev, Ivelina I Tomova, Maida M Tiholova, Todor V Kantardjiev
INTRODUCTION: Campylobacter spp. are important causative agents of gastrointestinal infections in humans. The most frequently isolated strains of this bacterial genus are Campylobacter jejuni and Campylobacter coli. To date, genetic methods for bacterial identification have not been used in Bulgaria. We optimized the multiplex PSR assay to identify Campylobacter spp. and differentiate C. jejuni from C. coli in clinical isolates. We also compared this method with the routinely used biochemical methods...
April 2016: Folia Medica
https://www.readbyqxmd.com/read/27200686/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-costs-and-health-care-resource-consumption-data
#3
P Laires, J Conceição, F Araújo, J Dores, C Silva, L Radican, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27200579/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-clinical-outcomes-in-admitted-patients
#4
J Conceição, J Dores, F Araújo, P Laires, R Carr, K Brodovicz, L Radican, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27200577/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-clinical-outcomes-in-the-emergency-room
#5
J Conceição, J Dores, F Araújo, P Laires, R Carr, K Brodovicz, L Radican, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27200576/hipos-er-hypoglycemia-in-portugal-observational-study-emergency-room-outcomes-with-different-anti-hyperglycemic-agents
#6
J Conceição, P Laires, J Dores, F Araújo, R Carr, K Brodovicz, L Radican, V Vicente, A M Nogueira
No abstract text is available yet for this article.
November 2014: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/26783018/comparison-of-antimicrobial-susceptibility-of-campylobacter-strains-isolated-from-food-samples-and-patients-with-diarrhea
#7
COMPARATIVE STUDY
Bita Bakhshi, Amin Naseri, Masoud Alebouyeh
BACKGROUND: Campylobacter infections may lead to serious conditions, including septicemia or other invasive forms of the disease, which require rapid and accurate laboratory diagnosis and subsequently appropriate antimicrobial therapy. The aim of this study was to compare the species distribution and antimicrobial susceptibility pattern of Campylobacter spp. strains isolated from patients and food samples. METHODS: Biochemical identification was performed on 15 clinical and 30 food isolates of Campylobacter recovered onto Brucella agar containing 5% sheep blood...
2016: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/26602171/comparison-of-antimicrobial-susceptibility-of-campylobacter-strains-isolated-from-food-samples-and-patients-with-diarrhea
#8
Bita Bakhshi, Amin Naseri, Masoud Alebouyeh
BACKGROUND: Campylobacter infections may lead to serious conditions, including septicemia or other invasive forms of the disease, which require rapid and accurate laboratory diagnosis and subsequently appropriate antimicrobial therapy. The aim of this study was to compare the species distribution and antimicrobial susceptibility pattern of Campylobacter spp. strains isolated from patients and food samples. METHODS: Biochemical identification was performed on 15 clinical and 30 food isolates of Campylobacter recovered onto Brucella agar containing 5% sheep blood...
November 25, 2015: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/26561276/comparison-of-dose-and-catheter-optimization-algorithms-in-prostate-high-dose-rate-brachytherapy
#9
COMPARATIVE STUDY
Eric Poulin, Nicolas Varfalvy, Sylviane Aubin, Luc Beaulieu
PURPOSE: The purpose of this work was to compare the hybrid inverse treatment planning optimization (HIPO), inverse dose-volume histogram-based optimization (DVHO), and fast simulated annealing stochastic algorithm (IPSA). The catheter optimization algorithm HIPO was also compared with the Centroidal Voronoi Tessellation (CVT) algorithm. METHODS AND MATERIALS: In this study, eight high-dose-rate prostate cases were randomly selected from an anonymized bank of patients...
January 2016: Brachytherapy
https://www.readbyqxmd.com/read/26504034/dosimetric-and-radiobiological-evaluation-of-hybrid-inverse-planning-and-optimization-for-cervical-cancer-brachytherapy
#10
Lucílio Dos S Matias, Tomas Palmqvist, Jeanette Wolke, Josef Nilsson, Catharina Beskow, Alexandre M Maphossa, Iuliana Toma-Dasu
AIM: To compare manual graphical optimization (GrO) with hybrid inverse planning optimization (HIPO) of cervical cancer brachytherapy treatment plans using physical and radiobiological tools. PATIENTS AND METHODS: Ten patients suffering from cervical cancer, treated with pulsed brachytherapy using GrO plans, were included in the study. For each patient, four different HIPO class solutions with different dose objectives to the target and constraints to the organs at risk (OAR) produced four optimized plans, that were each compared to the corresponding GrO plan...
November 2015: Anticancer Research
https://www.readbyqxmd.com/read/26394042/differentiation-of-campylobacter-jejuni-and-campylobacter-coli-using-multiplex-pcr-and-high-resolution-melt-curve-analysis
#11
Banya Banowary, Van Tuan Dang, Subir Sarker, Joanne H Connolly, Jeremy Chenu, Peter Groves, Michelle Ayton, Shane Raidal, Aruna Devi, Thiru Vanniasinkam, Seyed A Ghorashi
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolates. A segment of the hippuricase gene (hipO) of C. jejuni and putative aspartokinase (asp) gene of C. coli were amplified from 26 Campylobacter isolates and amplicons were subjected to HRM curve analysis...
2015: PloS One
https://www.readbyqxmd.com/read/26187833/real-time-pcr-detection-of-campylobacter-spp-a-comparison-to%C3%A2-classic-culturing-and-enrichment
#12
COMPARATIVE STUDY
P de Boer, H Rahaoui, R J Leer, R C Montijn, J M B M van der Vossen
The major disadvantage of the current gold standard for detection of the food pathogen Campylobacter, i.e. culturing, is the lengthy procedure. In this study we assessed the use of real-time PCR for detection of Campylobacter. To this end, 926 poultry samples, taken from transport containers and broiler caeca in The Netherlands in 2007, were subjected to three different real-time PCR detection methods: one targeting the Campylobacter jejuni hipO gene, one targeting the Campylobacter coli glyA gene, and one generically targeting Campylobacter spp...
October 2015: Food Microbiology
https://www.readbyqxmd.com/read/25866115/loop-mediated-isothermal-amplification-lamp-for-detection-of-campylobacter-jejuni-and-c-coli-in-thai-children-with-diarrhea
#13
Ngan Thi Kim Pham, Quang Duy Trinh, Pattara Khamrin, Nuthapong Ukarapol, Tipachan Kongsricharoern, Wataru Yamazaki, Shihoko Komine-Aizawa, Shoko Okitsu, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima
Campylobacter species are common causes of bacterial diarrhea, and Campylobacter jejuni and C. coli are known as the predominant causative agents in humans. Recent studies suggested that loop-mediated isothermal amplification (LAMP) is an efficient and practical tool for rapid detection of C. jejuni and C. coli in clinical samples. We used LAMP to screen 151 stool samples for Campylobacter; these samples were collected in 2012 from Thai children with diarrhea. The PCR method discriminated C. jejuni and C. coli among the detected Campylobacter strains; these species were subjected to sequencing of the hipO gene (in C...
2015: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/25731001/identification-of-campylobacter-species-isolates-with-phenotypic-methods-and-polymerase-chain-reaction
#14
Biljana Miljković-Selimović, Tatjana Babić, Branislava Kocić, Aleksandra Matkić, Ljiljana Ristić
INTRODUCTION: Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) are the most common bacterial causes of enterocolitis in humans. However, identification of the species level is not always possible using standard biochemical tests. OBJECTIVE: Therefore, the goal of this study was to identify these microorganisms by both phenotyping and polymerase chain reaction (PCR) technique. METHODS: A total of 153 species of thermophilic campylobacters were examined with standard biochemical tests and PCR technique to prove hipO genes of C...
November 2014: Srpski Arhiv za Celokupno Lekarstvo
https://www.readbyqxmd.com/read/25672405/isolation-and-characterization-of-campylobacter-strains-from-diarrheal-patients-in-central-and-suburban-bangkok-thailand
#15
Worada Samosornsuk, Masahiro Asakura, Emi Yoshida, Takashi Taguchi, Bunchuay Eampokalap, Wanpen Chaicumpa, Shinji Yamasaki
Campylobacter-induced diarrhea is increasingly recognized worldwide. However, little information is available regarding the Campylobacter strains associated with diarrheal patients in Thailand. In this study, we attempted to isolate Campylobacter strains from diarrheal patients in Thailand and to characterize the species using a cytolethal distending toxin (cdt) gene-based C. jejuni, C. coli, and C. fetus-specific multiplex PCR assay. Campylobacter species were also confirmed using 16S rRNA gene sequencing and hipO gene detection...
2015: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/25493531/comparison-of-ipsa-and-hipo-inverse-planning-optimization-algorithms-for-prostate-hdr-brachytherapy
#16
Vanessa Panettieri, Ryan L Smith, Natasha J Mason, Jeremy L Millar
Publications have reported the benefits of using high-dose-rate brachytherapy (HDRB) for the treatment of prostate cancer, since it provides similar biochemical control as other treatments while showing lowest long-term complications to the organs at risk (OAR). With the inclusion of anatomy-based inverse planning opti- mizers, HDRB has the advantage of potentially allowing dose escalation. Among the algorithms used, the Inverse Planning Simulated Annealing (IPSA) optimizer is widely employed since it provides adequate dose coverage, minimizing dose to the OAR, but it is known to generate large dwell times in particular positions of the catheter...
2014: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/25447341/a-comparison-of-inverse-optimization-algorithms-for-hdr-pdr-prostate-brachytherapy-treatment-planning
#17
COMPARATIVE STUDY
Anna M Dinkla, Rob van der Laarse, Emmie Kaljouw, Bradley R Pieters, Kees Koedooder, Niek van Wieringen, Arjan Bel
PURPOSE: Graphical optimization (GrO) is a common method for high-dose-rate/pulsed-dose-rate (PDR) prostate brachytherapy treatment planning. New methods performing inverse optimization of the dose distribution have been developed over the past years. The purpose is to compare GrO and two established inverse methods, inverse planning simulated annealing (IPSA) and hybrid inverse treatment planning and optimization (HIPO), and one new method, enhanced geometric optimization-interactive inverse planning (EGO-IIP), in terms of speed and dose-volume histogram (DVH) parameters...
March 2015: Brachytherapy
https://www.readbyqxmd.com/read/25198853/development-of-a-loop-mediated-isothermal-amplification-assay-for-rapid-sensitive-detection-of-campylobacter-jejuni-in-cattle-farm-samples
#18
Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho
Campylobacter jejuni is a leading cause of bacterial foodborne disease worldwide. The detection of this organism in cattle and their environment is important for the control of C. jejuni transmission and the prevention of campylobacteriosis. Here, we describe the development of a rapid and sensitive method for the detection of C. jejuni in naturally contaminated cattle farm samples, based on real-time loop-mediated isothermal amplification (LAMP) of the hipO gene. The LAMP assay was specific (100% inclusivity and exclusivity for 84 C...
September 2014: Journal of Food Protection
https://www.readbyqxmd.com/read/25178595/multiplex-polymerase-chain-reaction-for-detection-of-campylobacter-from-stool-specimen
#19
S R Sarkar, N C Ray, M A Hossain, S K Paul, S Sarkar, N Kobayashi
This was a study to prospectively evaluate the sensitivity and specificity of multiplex polymerase chain reaction (PCR) to identify Campylobacter. Multiplex polymerase chain reaction (PCR) based on cadF, hipO & asp gene for Campylobacter genus, C. jejuni & C. coli were tested for detection of Campylobacter jejuni & C. coli in naturally infected faecal samples of human. All the samples were subjected to the cultural isolation of organism and biochemical characterization. The samples resulted in the amplification of a DNA fragment of size 400 bp, 500 bp &735 bp in PCR assay...
July 2014: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/25030318/a-series-of-3d-metal-organic-frameworks-based-on-24-mc-6-metallacrown-clusters-structure-magnetic-and-luminescence-properties
#20
Kai Wang, Hua-Hong Zou, Zi-Lu Chen, Zhong Zhang, Wei-Yin Sun, Fu-Pei Liang
Four isostructural metal organic frameworks (MOFs), namely [Co6(HipO)6]·6H2O (1), [Mn6(HipO)6]·6H2O (2), [Cd6(HipO)6]·6H2O (3) and [Zn6(HipO)6]·7H2O (4) (H3ipO = 2-hydroxyisophthalic acid), were synthesized and structurally characterized. They have a 3D (4,6)-connected framework based on [24-MC-6] metallacrown clusters ([24-MC-6]-based MOFs). The arrangements of the 24-MC-6 metallacrown SBUs show a regular change indicated by the orientation of their symmetry axes, resulting in a special dense packing mode different from other [24-MC-6]-based MOFs...
September 14, 2014: Dalton Transactions: An International Journal of Inorganic Chemistry
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