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https://www.readbyqxmd.com/read/29164713/importance-of-oil-overlay-for-production-of-porcine-embryos-in-vitro
#1
REVIEW
C A Martinez, E A Martinez, M A Gil
Technologies to edit the zygote genome have revolutionized biomedical research not only for the creation of animal models for the study of human disease but also for the generation of functional human cells and tissues through interspecies blastocyst complementation technology. The pig is the ideal species for these purposes due to its great similarity in anatomy and physiology to humans. Emerging biotechnologies require the use of oocytes and/or embryos of good quality, which might be obtained using in vitro production (IVP) techniques...
November 21, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29138591/anatomical-etiology-of-pseudo-sciatica-from-superior-cluneal-nerve-entrapment-a-laboratory-investigation
#2
Tomoyuki Konno, Yoichi Aota, Hiroshi Kuniya, Tomoyuki Saito, Ning Qu, Shogo Hayashi, Shinichi Kawata, Masahiro Itoh
Objective: The superior cluneal nerve (SCN) may become entrapped where it pierces the thoracolumbar fascia over the iliac crest; this can cause low back pain (LBP) and referred pain radiating into the posterior thigh, calf, and occasionally the foot, producing the condition known as "pseudo-sciatica." Because the SCN was thought to be a cutaneous branch of the lumbar dorsal rami, originating from the dorsal roots of L1-L3, previous anatomical studies failed to explain why SCN causes "pseudo-sciatica"...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/29138215/physiology-undergraduate-degree-requirements-in-the-u-s
#3
Valerie S VanRyn, James M Poteracki, Erica A Wehrwein
Course-level learning objectives and core concepts for undergraduate physiology teaching exist. The next step is to consider how these resources fit into generalizable program-level guidelines for Bachelor of Science (BS) degrees in Physiology. In the absence of program-level guidelines for Physiology degree programs, we compiled a selective internal report to review degree requirements from 18 peer BS programs entitled "Physiology" in the United States (U.S.). There was a range of zero to three required semesters of math, physics, physics laboratory, general biology, biology laboratory, general chemistry, chemistry laboratory, organic chemistry, organic chemistry laboratory, biochemistry, biochemistry laboratory, anatomy, anatomy laboratory, core systems physiology, and physiology laboratory...
December 1, 2017: Advances in Physiology Education
https://www.readbyqxmd.com/read/29127734/population-representation-among-anatomical-donors-and-the-implication-for-medical-student-education
#4
Amanda J Collins, Whitney Smith, Eustathia Lela Giannaris, Elizabeth Orvek, Peter Lazar, Jan K Carney, Anne M Gilroy, Max P Rosen
INTRODUCTION: Dissection provides a unique opportunity to integrate anatomical and clinical education. Commonly, cadavers are randomly assigned to courses, which may result in skewed representation of patient populations. The primary aim of this study was to determine if the anatomical donors studied by students at Medical School (MS) accurately represent the disease burden of the local patient population. MATERIALS AND METHODS: This cross-sectional study compared Medical Center patient claims data and body donation data from the Anatomical Gift Program (AGP)...
November 11, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/29112797/exploring-anatomy-and-physiology-using-ipad-applications
#5
Tandra R Chakraborty, Deborah F Cooperstein
This study examined the use of iPads with anatomy applications (apps) in the laboratory sections of the largest undergraduate course at the university, Anatomy and Physiology, serving more than 300 students. The majority of these students were nursing, exercise science/physical education and biology majors. With a student survey (student opinion) and student practicum grades as metrics, this study determined whether the introduction of this novel mobile technology improved student grades and aided the students in learning the course material...
November 7, 2017: Anatomical Sciences Education
https://www.readbyqxmd.com/read/29101369/trabecular-bone-anisotropy-imaging-with-a-compact-laser-undulator-synchrotron-x-ray-source
#6
Christoph Jud, Eva Braig, Martin Dierolf, Elena Eggl, Benedikt Günther, Klaus Achterhold, Bernhard Gleich, Ernst Rummeny, Peter Noël, Franz Pfeiffer, Daniela Muenzel
Conventional x-ray radiography is a well-established standard in diagnostic imaging of human bones. It reveals typical bony anatomy with a strong surrounding cortical bone and trabecular structure of the inner part. However, due to limited spatial resolution, x-ray radiography cannot provide information on the microstructure of the trabecular bone. Thus, microfractures without dislocation are often missed in initial radiographs, resulting in a lack or delay of adequate therapy. Here we show that x-ray vector radiography (XVR) can overcome this limitation and allows for a deeper insight into the microstructure with a radiation exposure comparable to standard radiography...
November 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29100631/drug-cues-conditioned-reinforcement-and-drug-seeking-the-sequelae-of-a-collaborative-venture-with-athina-markou
#7
REVIEW
Barry J Everitt
Athina Markou spent a research period in my laboratory, then in the Department of Anatomy in Cambridge University, in 1991 to help us establish a cocaine-seeking procedure. Thus we embarked on developing a second-order schedule of intravenous cocaine reinforcement to investigate the neural basis of the pronounced effects of cocaine-associated conditioned stimuli on cocaine seeking. This brief review summarizes the fundamental aspects of cocaine seeking measured using this approach and the importance of the methodology in enabling us to define the neural mechanisms and circuitry underlying conditioned reinforcement and cocaine, heroin, and alcohol seeking...
September 25, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/29096042/what-type-of-learner-are-your-students-preferred-learning-styles-of-undergraduate-gross-anatomy-students-according-to-the-index-of-learning-styles-questionnaire
#8
Melissa M Quinn, Theodore Smith, Eileen L Kalmar, Jennifer M Burgoon
Students learn and process information in many different ways. Learning styles are useful as they allow instructors to learn more about students, as well as aid in the development and application of useful teaching approaches and techniques. At the undergraduate level there is a noticeable lack of research on learning style preferences of students enrolled in gross anatomy courses. The Index of Learning Styles (ILS) questionnaire was administered to students enrolled in a large enrollment undergraduate gross anatomy course with laboratory to determine their preferred learning styles...
November 2, 2017: Anatomical Sciences Education
https://www.readbyqxmd.com/read/29095669/the-anatomy-of-a-nonfaradaic-electrochemical-biosensor
#9
Hunter Stevenson, Nandhinee Radha Shanmugam, Anjan Paneer Selvam, Shalini Prasad
Point-of-care (POC) testing has revolutionized diagnostic healthcare, bringing medical results directly and immediately to the patient. With faster diagnostics, more immediate clinical management decisions can be made. POC tests most often use a dipstick or swab format to detect the presence of a pathogen, disease, or other relevant biomarker. In these formats, the POC tests eliminate the need for complex lab equipment and trained personnel to collect, process, and analyze sample data for simple diagnostics...
November 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/29091339/undergraduate-dental-students-perceptions-of-head-and-neck-anatomy-teaching-in-australia
#10
N M Hassan, R Akhter, H Al-Aubaidy, A Flatau
INTRODUCTION: Most Australian universities have abbreviated the anatomy curriculum and modified its mode of delivery. This study examines dental student perceptions of different methods of head and neck anatomy teaching with respect to the adequacy of allocated time and the relative meaningfulness of the teaching methods. METHODS: All second-year students in the School of Dentistry and Health Sciences at Charles Sturt University (CSU) were invited to complete a matrix-grid questionnaire...
November 1, 2017: European Journal of Dental Education: Official Journal of the Association for Dental Education in Europe
https://www.readbyqxmd.com/read/29089283/occurrence-and-classification-of-proximal-type-i-endoleaks-after-endovascular-aneurysm-sealing-using-the-nellix%C3%A2-device
#11
Leo H van den Ham, Andrew Holden, Janis Savlovskis, Andrew Witterbottom, Kenneth Ouriel, Michel M P J Reijnen
OBJECTIVE/BACKGROUND: Proximal type I endoleaks are associated with abdominal aortic aneurysm (AAA) growth and rupture and necessitate repair. The Nellix™ EndoVascular Aneurysm Sealing (EVAS) system is a unique approach to AAA repair, where the appearance and treatment of endoleaks is also different. This study aimed to analyse and categorise proximal endoleaks in an EVAS treated cohort. METHODS: All patients, treated from February 2013 to December 2015, in 15 experienced EVAS centres, presenting with proximal endoleak were included...
October 28, 2017: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29082319/anatomy-of-a-joint-comparing-self-reported-and-actual-dose-of-cannabis-and-tobacco-in-a-joint-and-how-these-are-influenced-by-controlled-acute-administration
#12
Chandni Hindocha, Tom P Freeman, H Valerie Curran
Introduction: Major gaps exist in the measurement of cannabis exposure. The accuracy of self-reported cannabis and tobacco dose per joint is poorly characterized and has never been investigated following acute cannabis/tobacco exposure. Using an innovative "Roll a Joint" paradigm, this study aims to (1) compare estimated and actual dose of cannabis and tobacco per joint at baseline and (2) examine the acute effects of cannabis and/or tobacco on estimated and actual dose. Materials and Methods: We investigated this by using a randomized, double-blind, placebo-controlled crossover 2 (active cannabis, placebo cannabis)×2 (active tobacco, placebo tobacco) design in a laboratory setting...
2017: Cannabis and Cannabinoid Research
https://www.readbyqxmd.com/read/29077751/use-of-ferrets-for-electrophysiologic-monitoring-of-ion-transport
#13
Niroop Kaza, S Vamsee Raju, Joan M Cadillac, John A Trombley, Lawrence Rasmussen, Liping Tang, Erik Dohm, Kevin S Harrod, Steven M Rowe
Limited success achieved in translating basic science discoveries into clinical applications for chronic airway diseases is attributed to differences in respiratory anatomy and physiology, poor approximation of pathologic processes, and lack of correlative clinical endpoints between humans and laboratory animal models. Here, we discuss advantages of using ferrets (Mustela putorus furo) as a model for improved understanding of human airway physiology and demonstrate assays for quantifying airway epithelial ion transport in vivo and ex vivo, and establish air-liquid interface cultures of ferret airway epithelial cells as a complementary in vitro model for mechanistic studies...
2017: PloS One
https://www.readbyqxmd.com/read/29054286/functional-roles-of-orexin-hypocretin-receptors-in-reward-circuit
#14
Abbas Haghparast, Zahra Fatahi, Reza Arezoomandan, Sara Karimi, Zahra Taslimi, Shahram Zarrabian
Since its first discovery in 1998, it has become clear that the orexinergic system plays an important role in regulating a number of functions including food, sex, social connections, and most prominently reward-related behaviors. Orexinergic neurons in the lateral hypothalamus project extensively to other brain areas, two most important of which are the ventral tegmental area and the nucleus accumbens that are involved in reward processing. In this review, we have presented the work in our laboratory along with the work of others and have discussed the possible functions we can infer from the research...
2017: Progress in Brain Research
https://www.readbyqxmd.com/read/29045057/proper-training-in-laparoscopic-hernia-repair-is-necessary-to-minimize-the-rising-recurrence-rate-in-japan
#15
Sumio Matsumoto, Tetsushi Hayakawa, Yoh Kawarada, Kazunori Uchida, Tohru Eguchi, Hidetoshi Wada, Nozomi Ueno, Hitoshi Idani, Kanyu Nakano, Yoshiyuki Oomomo
INTRODUCTION: The Japan Society for Endoscopic Surgery reported that the recurrence rate for inguinal hernia repair in Japan was less than 1% in 2010. However, its 2012 survey found that the recurrence rate had increased to 4% for the transabdominal preperitoneal procedure and 5% for the totally extraperitoneal procedure. We held 14 hernia repair training courses from 2011 to 2016 with help from Covidien. This study aimed to determine the effect of this training on the recurrence rate...
October 17, 2017: Asian Journal of Endoscopic Surgery
https://www.readbyqxmd.com/read/29037824/resource-usage-implementing-the-surgical-resident-prep-curriculum-at-a-single-institution
#16
Lily Owei, Chris Neylan, Rachel Kelz, Kristoffel R Dumon, Steve R Allen, Noel Williams, Daniel T Dempsey, Carla Fisher
OBJECTIVE: The Resident Prep Curriculum (RPC), published in 2014 and developed as a collaboration of the American College of Surgeons, Association of Program Directors in Surgery, and the Association for Surgical Education, was designed to improve the quality and consistency of medical student preparation for surgical residency. We aim to assess the feasibility of and resource usage for implementation of this curriculum at our institution. DESIGN: Our institution expanded upon a pre-existing 2-week surgical preparatory course, adding modules designed to meet the goals and objectives of the RPC...
October 13, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/29024453/the-histopathologic-reliability-of-tissue-taken-from-cadavers-within-the-gross-anatomy-laboratory
#17
Guenevere Rae, William P Newman, Robin McGoey, Supriya Donthamsetty, Aryn C Karpinski, Jeffrey Green
The purpose of this study was to examine the histopathologic reliability of embalmed cadaveric tissue taken from the gross anatomy laboratory. Tissue samples from hearts, livers, lungs, and kidneys were collected after the medical students' dissection course was completed. All of the cadavers were embalmed in a formalin-based fixative solution. The tissue was processed, embedded in paraffin, sectioned at six micrometers, and stained with H&E. The microscope slides were evaluated by a board certified pathologist to determine whether the cellular components of the tissues were preserved at a high enough quality to allow for histopathologic diagnosis...
October 10, 2017: Anatomical Sciences Education
https://www.readbyqxmd.com/read/29016187/quantitative-and-qualitative-analysis-of-the-medial-patellar-ligaments-an-anatomic-and-radiographic-study
#18
Bradley M Kruckeberg, Jorge Chahla, Gilbert Moatshe, Mark E Cinque, Kyle J Muckenhirn, Jonathan A Godin, Taylor J Ridley, Alex W Brady, Elizabeth A Arendt, Robert F LaPrade
BACKGROUND: The qualitative and quantitative anatomy of the medial patellar stabilizers has been reported; however, a quantitative analysis of the anatomic and radiographic attachments of all 4 ligaments relative to anatomic and osseous landmarks, as well as to one another, has yet to be performed. PURPOSE: To perform a qualitative and quantitative anatomic and radiographic evaluation of the medial patellofemoral ligament (MPFL), medial patellotibial ligament (MPTL), medial patellomeniscal ligament (MPML), and medial quadriceps tendon femoral ligament (MQTFL) attachment sites, with attention to their relationship to pertinent osseous and soft tissue landmarks...
October 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28986008/characterisation-of-the-porcine-eyeball-as-an-in-vitro-model-for-dry-eye
#19
Francesco Menduni, Leon N Davies, D Madrid-Costa, Antonio Fratini, James S Wolffsohn
PURPOSE: To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye. METHODS: Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs. RESULTS: Corneal steepest meridian was 7.85±0.32mm, corneal flattest meridian was 8.28±0.32mm, shortest corneal diameter was 12...
October 3, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28985669/establishment-of-next-generation-neurosurgery-research-and-training-laboratory-with-integrated-human-performance-monitoring
#20
Antonio Bernardo
Quality of neurosurgical care and patient outcomes are inextricably linked to surgical and technical proficiency and a thorough working knowledge of microsurgical anatomy. Neurosurgical laboratory-based cadaveric training is essential for the development and refinement of technical skills before their use on a living patient. Recent biotechnological advances including 3-dimensional (3D) microscopy and endoscopy, 3D printing, virtual reality, surgical simulation, surgical robotics, and advanced neuroimaging have proved to reduce the learning curve, improve conceptual understanding of complex anatomy, and enhance visuospatial skills in neurosurgical training...
October 2017: World Neurosurgery
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