Jenny Kaur Singh, Rebecca Smith, Magdalena B Rother, Anton J L de Groot, Wouter W Wiegant, Kees Vreeken, Ostiane D'Augustin, Robbert Q Kim, Haibin Qian, Przemek M Krawczyk, Román González-Prieto, Alfred C O Vertegaal, Meindert Lamers, Sébastien Huet, Haico van Attikum
DNA double-strand breaks (DSBs) are among the most deleterious types of DNA damage as they can lead to mutations and chromosomal rearrangements, which underlie cancer development. Classical non-homologous end-joining (cNHEJ) is the dominant pathway for DSB repair in human cells, involving the DNA-binding proteins XRCC6 (Ku70) and XRCC5 (Ku80). Other DNA-binding proteins such as Zinc Finger (ZnF) domain-containing proteins have also been implicated in DNA repair, but their role in cNHEJ remained elusive. Here we show that ZNF384, a member of the C2H2 family of ZnF proteins, binds DNA ends in vitro and is recruited to DSBs in vivo...
November 12, 2021: Nature Communications