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resin blood culture

Jungho Kim, Hye-Young Wang, Seoyong Kim, Soon Deok Park, Kwangmin Yu, Hyo Youl Kim, Young Uh, Hyeyoung Lee
DNA extraction efficiency affects the success of PCR-based method applications. The Punch-it™ NA-Sample kit for extracting DNA by using paper chromatography is technically easy to use and requires just two reagents and only 10min to complete. The Punch-it™ NA-Sample kit could be offered as a rapid, accurate, and convenient method for extracting bacterial and fungal DNA from blood culture bottles. We compared the efficiencies of the commercial kit (Punch-it™ NA-Sample kit) and an in-house conventional boiling method with Chelex-100 resin for DNA extraction from blood culture bottles...
September 2016: Journal of Microbiological Methods
S Bailly, C Garnaud, M Cornet, P Pavese, R Hamidfar-Roy, L Foroni, S Boisset, J-F Timsit, D Maubon
The diagnosis and follow-up of candidemia still rely on blood cultures (BCs). In vitro studies show that antifungals can significantly modify the result of blood culture not containing adsorbing agents. We aimed to evaluate, under clinical conditions, the impact on BC yeast detection of systemic antifungal therapy (SAT). Patients (n = 125) experiencing candidemia at Grenoble University Hospital (France) were included in a 4-year retrospective study. The Plus Aerobic/F (Aerobic) and Plus Anaerobic/F (Anaerobic) bottles, which both contain adsorbing resins and the non-resin selective Mycosis IC/F (Mycosis) bottles, were compared using multivariate hierarchical models adjusted for clinical characteristics...
June 2016: European Journal of Clinical Microbiology & Infectious Diseases
Policarp Hortolà
Some archaeological or ethnographic specimens are unavailable for direct examination using a scanning electron microscope (SEM) due to methodological obstacles or legal issues. In order to assess the feasibility of using SEM synthetic replicas for the identification of bloodstains (BSs) via morphology of red blood cells (RBCs), three fragments of different natural raw material (inorganic, stone; plant, wood; animal, shell) were smeared with peripheral human blood. Afterwards, molds and casts of the bloodstained areas were made using vinyl polysiloxane (VPS) silicone impression and polyurethane (PU) resin casting material, respectively...
November 2, 2015: Microscopy and Microanalysis
Bing Han, Xiao-Lei Shi, Yue Zhang, Zhong-Ze Gu, Xian-Wen Yuan, Hao-Zhen Ren, Yong Qiu, Yi-Tao Ding
BACKGROUND: A novel hybrid bioartificial liver (HBAL) was constructed using an anionic resin adsorption column and a multi-layer flat-plate bioreactor containing porcine hepatocytes co-cultured with bone marrow mesenchymal stem cells (MSCs). This study aimed to evaluate the microbiological safety of the HBAL by detecting the transmission of porcine endogenous retroviruses (PERVs) into canines with acute liver failure (ALF) undergoing HBAL. METHODS: Eight dogs with ALF received a 6-hour HBAL treatment on the first day after the modeling by D-galactosamine administration...
October 2015: Hepatobiliary & Pancreatic Diseases International: HBPD INT
Steven De Keukeleire, Ingrid Wybo, Kristof Emmerechts, Denis Piérard
Recently new resin-based BacT/Alert FAN Plus bottles containing antibiotic-binding polymeric beads, were introduced as an improvement of the charcoal-based FAN bottles for the recovery of bacteria and fungi. To assess the performance of the novel anaerobic FN plus bottles in the detection of anaerobic organisms, we compared the detection rate and the time to detection (TTD) in spiked resin-based FN Plus bottles and charcoal-based FN bottles. The bottles were experimentally seeded with reference strains or clinical strains collected from positive blood cultures...
October 2015: Anaerobe
Dan Li, Rui Hui, Yongwu Hu, Yan Han, Shuzhong Guo
OBJECTIVE: To investigate the effects of Dragon' s blood extract on proliferation and secret extracellular matrix function of fibroblasts in vitro. METHODS: Dragon' s blood was extracted by chloroform, acetoacetic ester, alcohol. Human fibroblast were cultured in vitro in media containing gradient dilutions of Dragon' s blood extracts (0.002, 0.02, 0.2, 2, 20 mg/ml) , which was followed by cell proliferation assessed with MTT assay on 0, 12, 24, 36, 48, 60, 72 h...
January 2015: Zhonghua Zheng Xing Wai Ke za Zhi, Zhonghua Zhengxing Waike Zazhi, Chinese Journal of Plastic Surgery
Lin-Chen Ho, Wei-Cheng Wu, Chang-Yu Chang, Hao-Hsuan Hsieh, Ching-Hsiao Lee, Huan-Tsung Chang
We have developed a simple, sensitive, and rapid fluorescence assay for the detection of cancer cells, based on "turn-on" retro-self-quenched fluorescence inside the cells. 1,3-Phenylenediamine resin (DAR) nanoparticles (NPs) containing rhodamine 6G (R6G) are conjugated with aptamer (apt) sgc8c to prepare sgc8c-R6GDAR NPs, while that containing rhodamine 101 (R101) are conjugated with TD05 for the preparation of TD05-R101DAR NPs. The sgc8c-R6GDAR and TD05-R101DAR NPs separately recognize CCRF-CEM and Ramos cells...
2015: Analytical Chemistry
Diana Couto, Rosa Sousa, Lara Andrade, Magdalena Leander, M Arturo Lopez-Quintela, José Rivas, Paulo Freitas, Margarida Lima, Graça Porto, Beatriz Porto, Félix Carvalho, Eduarda Fernandes
The use of iron oxide nanoparticles (ION) for diagnostic and therapeutic purposes requires a clear favorable risk-benefit ratio. This work was performed with the aim of studying the ability of polyacrylic acid (PAA)-coated and non-coated ION to induce genotoxicity in human T lymphocytes. For that purpose, their influence on cell cycle progression and on the induction of chromosome aberrations was evaluated. Blood samples collected from healthy human donors were exposed to PAA-coated and non-coated ION, at different concentrations, for 48h...
April 16, 2015: Toxicology Letters
Seyed Hamid Raji, Hasan Shojaei, Parinaz Saeidi Ghorani, Elahe Rafiei
BACKGROUND: The advantages of coated orthodontic wires such as esthetic and their effects on reduced friction, corrosion and allergic reaction and the significant consequences of plaque accumulation on oral health encouraged us to assess bacterial colonization on these wires. MATERIALS AND METHODS: A total of 18 (9 upper and 9 lower) epoxy resin coated 16 × 22 nickel-titanium wires (Spectra, GAC, USA) and 18 (9 upper and 9 lower) non-coated 16 × 22 nickel-titanium wires (Sentalloy, GAC, USA) with isolated packages were selected and sterilized before application...
November 2014: Dental Research Journal
Gökçe Meriç, Meryem Güvenir, Kaya Süer
OBJECTIVE: To evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials. BACKGROUND: Old denture wearer needs effective, easy-use and safe denture-cleaning material. MATERIAL AND METHODS: Square-shaped, heat-polymerised acrylic resin specimens (n = 550) were prepared and divided into five groups (n = 110 for each) corresponding to the microbial contamination (Candida albicans, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa)...
September 2016: Gerodontology
Wenchen Li, Qingsheng Liu, Lingyun Liu
We report two new amino acid based antifouling zwitterionic polymers, poly(N(4)-(2-methacrylamidoethyl)asparagine) (pAspAA) and poly(N(5)-(2-methacrylamidoethyl)glutamine) (pGluAA). The vinyl monomers were developed from aspartic acid and glutamic acid. Surface-initiated photoiniferter-mediated polymerization was employed to graft polymer brushes from gold surfaces. Different thickness of polymer brushes was controlled by varying UV irradiation time. The nonspecific adsorption from undiluted human blood serum and plasma was studied by surface plasmon resonance (SPR)...
October 28, 2014: Langmuir: the ACS Journal of Surfaces and Colloids
Andrew D Rape, Sanjay Kumar
Glioblastoma multiforme (GBM), the most prevalent primary brain cancer, is characterized by diffuse infiltration of tumor cells into brain tissue, which severely complicates surgical resection and contributes to tumor recurrence. The most rapid mode of tissue infiltration occurs along blood vessels or white matter tracts, which represent topological interfaces thought to serve as "tracks" that speed cell migration. Despite this observation, the field lacks experimental paradigms that capture key features of these tissue interfaces and allow reductionist dissection of mechanisms of this interfacial motility...
October 2014: Biomaterials
Barbara Fiori, Tiziana D'Inzeo, Viviana Di Florio, Flavio De Maio, Giulia De Angelis, Alessia Giaquinto, Lara Campana, Eloisa Tanzarella, Mario Tumbarello, Massimo Antonelli, Maurizio Sanguinetti, Teresa Spanu
We compared the clinical performances of the BacT/Alert Plus (bioMérieux) and Bactec Plus (Becton Dickinson) aerobic and anaerobic blood culture (BC) media with adsorbent polymeric beads. Patients ≥ 16 years old with suspected bloodstream infections (BSIs) were enrolled in intensive care units and infectious disease wards. A single 40-ml blood sample was collected from each and used to inoculate (10 ml/bottle) one set of BacT/Alert Plus cultures and one set of Bactec Plus cultures, each set consisting of one aerobic and one anaerobic bottle...
October 2014: Journal of Clinical Microbiology
Jintao Yang, Mingzhen Zhang, Hong Chen, Yung Chang, Zhan Chen, Jie Zheng
Numerous biocompatible antifouling polymers have been developed for a wide variety of fundamental and practical applications in drug delivery, biosensors, marine coatings, and many other areas. Several antifouling mechanisms have been proposed, but the exact relationship among molecular structure, surface hydration property, and antifouling performance of antifouling polymers still remains elusive. Here this work strives to provide a better understanding of the structure-property relationship of poly(N-hydroxyalkyl acrylamide)-based materials...
August 11, 2014: Biomacromolecules
Farzaneh Sharifiaghdas, Farzam Tajalli, Hasan Otukesh, Rozita Hosseini Shamsabadi
OBJECTIVE: Our aim was to evaluate the efficacy and safety of single endoscopic injection of Vantris in young girls affected by primary vesicoureteral reflux (VUR) at more than 2 years of prospective follow-up. MATERIAL AND METHODS: Over the last 4 years, 73 girls with primary VUR of grades (G) I-IV underwent a single endoscopic injection of Vantris. The mean age was 8.48 (SD=4.8) years. VUR was unilateral in 73 and bilateral in 13 patients, comprising 86 renal refluxing units (RRUs)...
December 2014: Journal of Pediatric Urology
Danijela Marovic, Antonija Tadin, Marin Mladinic, Danijela Juric-Kacunic, Nada Galic
PURPOSE: To simultaneously evaluate the genotoxicity of dental composites and adhesive systems in vitro using a cytogenetic assay, with respect to the influence of composite shade. METHODS: Genotoxicity assessment was carried out in human peripheral blood leukocytes using the comet assay. Three resin composite materials, two microhybrids and one nano-hybrid, in shade A1 and A3.5 were used with manufacturer-recommended four adhesive systems. Cultures were treated for 48 hours with samples after elusion for 1 hour, 1 day, 7 days or 30 days, in two different concentrations (4...
February 2014: American Journal of Dentistry
Timothy J Gray, Lee Thomas, Tom Olma, David H Mitchell, Jon R Iredell, Sharon C A Chen
An important role of the clinical microbiology laboratory is to provide rapid identification of bacteria causing bloodstream infection. Traditional identification requires the sub-culture of signaled blood culture broth with identification available only after colonies on solid agar have matured. MALDI-TOF MS is a reliable, rapid method for identification of the majority of clinically relevant bacteria when applied to colonies on solid media. The application of MALDI-TOF MS directly to blood culture broth is an attractive approach as it has potential to accelerate species identification of bacteria and improve clinical management...
2014: Journal of Visualized Experiments: JoVE
Lena S Mayer, Peter Uciechowski, Sören Meyer, Tanja Schwerdtle, Lothar Rink, Hajo Haase
Zinc deficiency has a fundamental influence on the immune defense, with multiple effects on different immune cells, resulting in a major impairment of human health. Monocytes and macrophages are among the immune cells that are most fundamentally affected by zinc, but the impact of zinc on these cells is still far from being completely understood. Therefore, this study investigates the influence of zinc deficiency on monocytes of healthy human donors. Peripheral blood mononuclear cells, which include monocytes, were cultured under zinc deficient conditions for 3 days...
July 2014: Metallomics: Integrated Biometal Science
M Pilar Calatayud, Beatriz Sanz, Vittoria Raffa, Cristina Riggio, M Ricardo Ibarra, Gerardo F Goya
Nanoparticles engineered for biomedical applications are meant to be in contact with protein-rich physiological fluids. These proteins are usually adsorbed onto the nanoparticle's surface, forming a swaddling layer that has been described as a 'protein corona', the nature of which is expected to influence not only the physicochemical properties of the particles but also the internalization into a given cell type. We have investigated the process of protein adsorption onto different magnetic nanoparticles (MNPs) when immersed in cell culture medium, and how these changes affect the cellular uptake...
August 2014: Biomaterials
Shahin Kasraei, Lida Sami, Sareh Hendi, Mohammad-Yousef Alikhani, Loghman Rezaei-Soufi, Zahra Khamverdi
OBJECTIVES: Recurrent caries was partly ascribed to lack of antibacterial properties in composite resin. Silver and zinc nanoparticles are considered to be broad-spectrum antibacterial agents. The aim of the present study was to evaluate the antibacterial properties of composite resins containing 1% silver and zinc-oxide nanoparticles on Streptococcus mutans and Lactobacillus. MATERIALS AND METHODS: Ninety discoid tablets containing 0%, 1% nano-silver and 1% nano zinc-oxide particles were prepared from flowable composite resin (n = 30)...
May 2014: Restorative Dentistry & Endodontics
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