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Nuclear glutathione

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https://www.readbyqxmd.com/read/29679905/qing-brick-tea-qbt-aqueous-extract-protects-monosodium-glutamate-induced-obese-mice-against-metabolic-syndrome-and-involves-up-regulation-transcription-factor-nuclear-factor-erythroid-2-related-factor-2-nrf2-antioxidant-pathway
#1
Wenqi Gao, Changyi Xiao, Jun Hu, Biaoxin Chen, Chunyan Wang, Bangping Cui, Pengyi Deng, Jian Yang, Zhifang Deng
BACKGROUND: Qing brick tea (QBT), traditional and popular beverage for Chinese people, is an important post-fermentation dark tea. Our present study was performed to investigate the ameliorative effects of QBT aqueous extract on metabolic syndrome (Mets) in monosodium glutamate-induced obese mice and the potential mechanisms. METHOD: Monosodium glutamate-induced obese mice were used to evaluate the anti-Mets effects of QBT. Content levels of malonaldehyde (MDA), reactive oxygen species (ROS) and protein carbonylation, antioxidant enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR) in the skeletal muscle were assessed by commercial kits, respectively...
April 18, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29678743/the-orphan-nuclear-receptor-nr4a1-attenuates-oxidative-stress-induced-%C3%AE-cells-apoptosis-via-up-regulation-of-glutathione-peroxidase-1
#2
Yingfeng Yang, Fangyu Xie, Dandan Qin, Chen Zong, Feng Han, Zeqing Pu, Dong Liu, Xia Li, Yuchao Zhang, Yuantao Liu, Xiangdong Wang
AIMS: Our previous study showed that NR4A1 protects against oxidative stress-induced cell apoptosis. However, the targets downstream of NR4A1 are incompletely known. Glutathione peroxidase 1 (GPX1) is the most common antioxidant enzyme in the glutathione peroxidase class. In this study, we aimed to investigate whether GPX1 is a mediator of the protective effects of NR4A1 in pancreatic β cells. MAIN METHODS: A pancreatic β cell line, MIN6, was used to generate NR4A1 over-expression cell line...
April 17, 2018: Life Sciences
https://www.readbyqxmd.com/read/29676772/functional-roles-of-eriocalyxin-b-in-zebrafish-revealed-by-transcriptome-analysis
#3
Xunian Zhou, Cyanne Ye Cao, Angel Tsz-Yau Wan, Grace Gar-Lee Yue, Frankie Hin-Fai Kwok, Kwok-Pui Fung, Handong Sun, Clara Bik-San Lau, Pema-Tenzin Puno, Stephen Kwok-Wing Tsui
BACKGROUND: Eriocalyxin B (EriB) is a natural ent-kaurane diterpenoid obtained from Isodon eriocalyx var. laxiflora (family Lamiaceae), which has multiple biological activities (e.g. anti-tumor and anti-inflammatory) via the alteration of gene expression and signaling transduction. Recently, RNA sequencing (RNA-seq) has been developed as a dynamic transcriptome approach to analyze the transcriptional profile and in addition use such gene expression profiles to identify novel candidate genes in a zebrafish model...
April 20, 2018: Molecular omics
https://www.readbyqxmd.com/read/29674077/detection-and-differentiation-of-cys-hcy-and-gsh-mixtures-by-19-f-nmr-probe
#4
Shengjun Yang, Qingbin Zeng, Qianni Guo, Shizhen Chen, Hongbin Liu, Maili Liu, Michael T McMahon, Xin Zhou
Simultaneous detection and differentiation of biomolecules is of significance in biological research. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. However, existing methods for simultaneous detection and differentiation of Cys, Hcy, and GSH are still challenging because of their similarity in structure and chemical properties. Herein we report a probe that simultaneously detects and discriminates between mixtures of Cys, Hcy and GSH using 19 F nuclear magnetic resonance (NMR)...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29672960/thai-herbal-antipyretic-22-formula-apf22-inhibits-uva-mediated-melanogenesis-through-activation-of-nrf2-regulated-antioxidant-defense
#5
Tasanee Onkoksoong, Saowanee Jeayeng, Naravat Poungvarin, Saowalak Limsaengurai, Onusa Thamsermsang, Pinpat Tripatara, Pravit Akarasereenont, Uraiwan Panich
Thai herbal antipyretic 22 formula (APF22), a polyherbal formula, has been traditionally used to treat dermatologic problems including hyperpigmentation. Exposure of the skin to ultraviolet A (UVA) causes abnormal melanin production induced by photooxidative stress. This study thus aimed to investigate the protective effects of APF22 extracts and phenolic compounds, ferulic acid (FA), and gallic acid (GA; used as positive control and reference compounds), on melanogenesis through modulation of nuclear factor E2-related factor 2 (Nrf2) signaling and antioxidant defenses in mouse melanoma (B16F10) cells exposed to UVA...
April 19, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29669072/vitamin-a-pretreatment-protects-no-induced-bovine-mammary-epithelial-cells-from-oxidative-stress-by-modulating-nrf2-and-nf-%C3%AE%C2%BAb-signaling-pathways
#6
H Y Shi, S M Yan, Y M Guo, B Q Zhang, X Y Guo, B L Shi
It is known that physiological overproduction of nitric oxide (NO) contributes to oxidative stress and inflammation. Our published studies indicated that vitamin A (VA) reduces NO-induced oxidative stress in bovine mammary epithelial cells (BMECs) by increasing antioxidant enzyme activities. However, the precise mechanism is unclear. The present study was conducted to examine the protective effects of VA on NO-induced damage to BMECs in vitro using diethylenetriamine nitric oxide (DETA-NO) as the NO donor and to explore the intracellular signaling mechanisms of VA that involve nuclear factor erythroid 2-related factor (Nrf2) and nuclear factor kappa-B (NF-κB)...
April 14, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29667773/benzyl-isothiocyanate-attenuates-the-hydrogen-peroxide-induced-interleukin-13-expression-through-glutathione-s-transferase-p-induction-in-t-lymphocytic-leukemia-cells
#7
Yue Tang, Sho Naito, Naomi Abe-Kanoh, Seiji Ogawa, Shu Yamaguchi, Beiwei Zhu, Yoshiyuki Murata, Yoshimasa Nakamura
We investigated the effect of benzyl isothiocyanate (BITC) on the hydrogen peroxide-induced gene expression of a T-helper-2 cytokine, interleukin (IL)-13, in T lymphocytic leukemia Jurkat cells. The 24-h pretreatment of BITC significantly inhibited the IL-13 expression enhanced by hydrogen peroxide. Although the BITC pretreatment did not change the enhanced level of the phosphorylated c-Jun N-terminal kinase (JNK), it significantly inhibited the nuclear translocation of c-Jun induced by hydrogen peroxide. BITC also increased the protein expression of glutathione S-transferase (GST) isozymes, GSTP1/2, as well as the total GST activity...
April 18, 2018: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/29663102/docosahexaenoic-acid-dha-provides-neuroprotection-in-traumatic-brain-injury-models-via-activating-nrf2-are-signaling
#8
Wei Zhu, Yuexia Ding, Wei Kong, Tuo Li, Hongguang Chen
In this study, we explored the neuroprotective effects of docosahexaenoic acid (DHA) in traumatic brain injury (TBI) models. In this study, we first confirmed that DHA was neuroprotective against TBI via the NSS test and Morris water maze experiment. Western blot was conducted to identify the expression of Bax, caspase-3, and Bcl-2. And the cell apoptosis of the TBI models was validated by TUNEL staining. Relationships between nuclear factor erythroid 2-related factor 2-antioxidant response element (Nrf2-ARE) pathway-related genes and DHA were explored by RT-PCR and Western blot...
April 16, 2018: Inflammation
https://www.readbyqxmd.com/read/29660436/in-vitro-study-of-doxorubicin-induced-oxidative-stress-in-spermatogonia-and-immature-sertoli-cells
#9
Amélie R Tremblay, Geraldine Delbes
Pediatric chemotherapy treatments can impair long-term male fertility. Unfortunately, no fertility preservation solution is available for pre-pubertal boys. Studies suggest that doxorubicin, used against pediatric cancers, induces oxidative stress in the testis. However, the targeted testicular cell types remain unknown. The goal of this study was to determine whether doxorubicin can induce oxidative stress in rat spermatogonia (GC-6Spg) and immature Sertoli (Ser-W3) cell lines, and to assess their protection by antioxidants...
April 13, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29660332/dusp14-knockout-accelerates-cardiac-ischemia-reperfusion-ir-injury-through-activating-nf-%C3%AE%C2%BAb-and-mapks-signaling-pathways-modulated-by-ros-generation
#10
Bin Lin, Jing Xu, De-Guang Feng, Feng Wang, Jia-Xiang Wang, Hui Zhao
Inflammation and oxidative stress are significantly involved in the progression of a variety of diseases, including myocardial ischemia/reperfusion (IR). In the present study, we hypothesized a protective role of dual-specificity phosphatase 14 (DUSP14) in myocardial IR, as well as the underlying molecular mechanism. The results indicated that DUSP14 was down-regulated following cardiac IR injury. Subsequently, the wild type (WT) and DUSP14-knockout (KO) mice were included to further reveal the potential role of DUSP14 in cardiac IR injury progression...
April 13, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29659168/metformin-alleviates-human-cellular-aging-by-upregulating-the-endoplasmic-reticulum-glutathione-peroxidase-7
#11
Jingqi Fang, Jiping Yang, Xun Wu, Gangming Zhang, Tao Li, Xi'e Wang, Hong Zhang, Chih-Chen Wang, Guang-Hui Liu, Lei Wang
Metformin, an FDA-approved antidiabetic drug, has been shown to elongate lifespan in animal models. Nevertheless, the effects of metformin on human cells remain unclear. Here, we show that low-dose metformin treatment extends the lifespan of human diploid fibroblasts and mesenchymal stem cells. We report that a low dose of metformin upregulates the endoplasmic reticulum-localized glutathione peroxidase 7 (GPx7). GP×7 expression levels are decreased in senescent human cells, and GPx7 depletion results in premature cellular senescence...
April 16, 2018: Aging Cell
https://www.readbyqxmd.com/read/29658582/sirt1-nrf2-signalling-pathway-prevents-cognitive-impairment-in-diabetic-rats-through-anti%C3%A2-oxidative-stress-induced-by-mirna%C3%A2-23b%C3%A2-3p-expression
#12
Jinhua Han, Xiaoning Liu, Yingzhi Li, Jun Zhang, Haichi Yu
In the present study the exact roles and mechanisms underlying the effect of miRNA‑23b‑3p on the cognitive impairment of diabetic rats were investigated. The in vivo model of diabetes was established in Wistar rats via a single injection of streptozotocin (STZ). Cognitive function was evaluated using a Morris water maze test. Oxidative stress was measured using ELISA kits, and the protein expression levels of B‑cell lymphoma 2‑associated X protein, silent information regulator 1 (SIRT1), nuclear factor erythroid 2‑related factor 2 (Nrf2) and GAPDH were measured by western blot analysis...
April 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29658369/hepatoprotective-effect-of-chiisanoside-against-acetaminophen-induced-acute-liver-injury-in-mice
#13
Xingbo Bian, Shijie Wang, Jinping Liu, Yan Zhao, Haijun Li, Lianxue Zhang, Pingya Li
This study was designed to investigate the hepatoprotective effect of chiisanoside (CSS) and its possible mechanisms on acetaminophen (APAP)-induced acute liver damage in mice. The serum activities of alanine transaminase (ALT), aspartate transaminase (AST), tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and the hepatic levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and malondialdehyde (MDA) were determined using the commercially available assay kits...
April 15, 2018: Natural Product Research
https://www.readbyqxmd.com/read/29656318/a-novel-tetrasubstituted-imidazole-as-a-prototype-for-the-development-of-anti-inflammatory-drugs
#14
Marcus Vinicius P S Nascimento, Antonio C M Munhoz, Lais C Theindl, Eduarda Talita B Mohr, Najla Saleh, Eduardo B Parisotto, Thaís A Rossa, Ariane Zamoner, Tania B Creczynski-Pasa, Fabíola B Filippin-Monteiro, Marcus M Sá, Eduardo Monguilhott Dalmarco
Although inflammation is a biological phenomenon that exists to protect the host against infections and/or related problems, its unceasing activation results in the aggravation of several medical conditions. Imidazoles, whether natural or synthetic, are molecules related to a broad spectrum of biological effects, including anti-inflammatory properties. In this study, we screened eight novel small molecules of the imidazole class synthesized by our research group for their in vitro anti-inflammatory activity...
April 14, 2018: Inflammation
https://www.readbyqxmd.com/read/29656184/ropivacaine-regulates-the-expression-and-function-of-heme-oxygenase-1
#15
Xiangbiao Yan, Yu Li, Xuechang Han, Xu Dong, Yajie Zhang, Qunzhi Xing
As a new generation of amide-type local anesthetics (LAs), ropivacaine has been widely used for pain management in clinical settings. Increasing evidence has shown that administration of ropivacaine causes cytotoxic effects and apoptosis. However, the underlying molecular mechanisms still need to be elucidated. In the current study, our results indicated that ropivacaine treatment caused a significant induction of heme oxygenase-1 (HO-1) at both the mRNA and protein levels in human SHSY5Y cells. Levels of HO-1 mRNA and protein peaked at 1 h and 18 h, respectively, in response to ropivacaine treatment...
April 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29652884/neochloris-oleoabundans-is-worth-its-salt-transcriptomic-analysis-under-salt-and-nitrogen-stress
#16
Lenny de Jaeger, Benoit M Carreres, Jan Springer, Peter J Schaap, Gerrit Eggink, Vitor A P Martins Dos Santos, Rene H Wijffels, Dirk E Martens
Neochloris oleoabundans is an oleaginous microalgal species that can be cultivated in fresh water as well as salt water. Using salt water gives the opportunity to reduce production costs and the fresh water footprint for large scale cultivation. Production of triacylglycerols (TAG) usually includes a biomass growth phase in nitrogen-replete conditions followed by a TAG accumulation phase under nitrogen-deplete conditions. This is the first report that provides insight in the saline resistance mechanism of a fresh water oleaginous microalgae...
2018: PloS One
https://www.readbyqxmd.com/read/29649787/defective-protein-repair-under-methionine-sulfoxide-a-deletion-drives-autophagy-and-are-dependent-gene-transcription
#17
Steven M Pennington, Paula R Klutho, Litao Xie, Kim Broadhurst, Olha M Koval, Michael L McCormick, Douglas R Spitz, Isabella M Grumbach
OBJECTIVE: Reduction of oxidized methionines is emerging as a major protein repair pathway. The lack of methionine sulfoxide reductase A (MsrA) exacerbates cardiovascular disease phenotypes driven by increased oxidative stress. However, the role of MsrA on maintaining cellular homeostasis in the absence of excessive oxidative stress is less well understood. METHODS AND RESULTS: Constitutive genetic deletion of MsrA increased formation of p62-containing protein aggregates, activated autophagy, and decreased a marker of apoptosis in vascular smooth muscle cells (VSMC)...
April 3, 2018: Redox Biology
https://www.readbyqxmd.com/read/29649627/overexpression-of-hipk2-attenuates-spinal-cord-injury-in-rats-by-modulating-apoptosis-oxidative-stress-and-inflammation
#18
Renbo Li, Jingbo Shang, Wei Zhou, Li Jiang, Donghui Xie, Guanjun Tu
HIPK2 is considered to be a tumor suppressor. It also has been implicated in several functions such as apoptosis and inflammation that are linked to spinal cord injury (SCI). However, whether HIPK2 ameliorates the neurological pain of SCI remains unclear. Here, we investigated the effects of HIPK2 on neurological function, oxidative stress, levels of inflammatory cytokines and expression of Bcl-2/Bax in an SCI model. Firstly, we evaluated the therapeutic effects of HIPK2 on neurological pain in the SCI rat using the Basso, Beattie and Bresnahan scores and H & E staining...
April 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29629712/l-theanine-protects-h9c2-cells-from-hydrogen-peroxide-induced-apoptosis-by-enhancing-antioxidant-capability
#19
Chengjian Li, Qiongxian Yan, Shaoxun Tang, Wenjun Xiao, Zhiliang Tan
BACKGROUND L-theanine is a non-protein amino acid in green tea, and its hepatoprotection and neuroprotection have been verified. However, whether L-theanine can prevent cardiomyocytes from apoptosis is unclear yet. This study evaluated the protective effects of L-theanine on H2O2-induced heart injury in vitro. MATERIAL AND METHODS The certified H9C2 cells were pretreated with L-theanine (0 mM, 4 mM, 8 mM, and 16 mM) for 24 h, followed by 160 µM H2O2 solution for 4 h. The cell viability and antioxidant indices were assayed...
April 9, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29626576/2-4-dihydroxy-6-methoxy-3-5-dimethylchalcone-a-potent-nrf2-are-pathway-inhibitor-reverses-drug-resistance-by-decreasing-glutathione-synthesis-and-drug-efflux-in-bel-7402-5-fu-cells
#20
Xing Wei, Xuejun Mo, Faliang An, Xiang Ji, Yanhua Lu
2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC) is a major active constituent of the buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry (Myrtaceae), a main ingredient of herbal tea in tropical zones. DMC has been reported to significantly reverse drug resistance in BEL-7402/5-FU cells. Glutathione (GSH) and glutathione S-transferase (GST) play important roles in an efflux system that protects the cells from anticancer drugs. In this study, DMC remarkably decreased the intracellular GSH content and GST activity...
April 4, 2018: Food and Chemical Toxicology
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