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Han Liu, Mingqian Li, Shunfeng Cai, Xinyi He, Yongqi Shao, Xingmeng Lu
Nosema bombycis is an obligate intracellular parasitic fungus that utilizes a distinctive mechanism to infect Bombyx mori Spore germination can be used for host cell invasion; however, the detailed mechanism remains to be elucidated. The ricin-B-lectin (RBL) gene is significantly differentially regulated after N. bombycis spore germination, and NbRBL might play roles in spore germination and infection. In this study, the biological function of NbRBL was examined. Protein sequence analysis showed that NbRBL is a secreted protein that attaches to carbohydrates...
September 20, 2016: Acta Biochimica et Biophysica Sinica
Y Zhang, Y Shi, H Yu, J Li, Y Quan, T Shu, Z Nie, Y Zhang, W Yu
Baculoviridae is a family of invertebrate viruses with large double-stranded DNA genomes. Proteins encoded by some late expression factor (lef ) genes are involved in the regulation of viral gene expression. Lef-9 is one of four transcription-specific Lefs, which are components of the virus-encoded RNA polymerase, and can initiate and transcribe late and very late genes. As a multifunctional protein encoded by the Bombyx mori nucleopolyhedrovirus (BmNPV), Lef-9 may be involved in the regulation of viral propagation...
2016: Acta Virologica
S Vidyalakshmi, R Madhavan Nirmal, V Veeravarmal, A Santhadevy, R Aravindhan, Sumathy
INTRODUCTION: The malignant transformation rate of Oral Lichen Planus (OLP) is between 0% and 5.8%. Oral lesions of lichen planus clinically presents itself multifocally, simulating the process of field cancerization in high risk malignancies. The Buccal MicroNucleus Cytome Assay (BMN Assay) provides a platform to identify the high risk individuals by evaluating the markers of nuclear damage at an earliest micro invasive phase. AIM: To evaluate DNA damage in exfoliated buccal mucosal cells in individuals with oral lichen planus lesions and thereby to delineate the high risk group...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
Rui-Ting Chen, Peng Jiao, Yan Lu, Hu-Hu Xin, Deng-Pan Zhang, Mei-Xian Wang, Shuang Liang, Yun-Gen Miao
The apoptosis mechanisms in mammals were investigated relatively clearly. However, little is known about how apoptosis is achieved at a molecular level in silkworm cells. We cloned a caspase homologous gene named BmDredd (where Bm is Bombyx mori and Dredd is death-related ced-3/Nedd2-like caspase) in BmN cells from the ovary of Bm and analyzed its biological information. We constructed the N-terminal, C-terminal, and overexpression vector of BmDredd, respectively. Our results showed that the transcriptional expression level of BmDredd was increased in the apoptotic BmN cells...
August 25, 2016: Archives of Insect Biochemistry and Physiology
Guohua Zhong, Gaofeng Cui, Xin Yi, Ranran Sun, Jingjing Zhang
The insecticide cytotoxicology, as a new branch of toxicology, has rapidly developed in China. During the past twenty years, thousands of investigations have sprung up to evaluate the damages and clarify the mechanisms of insecticidal chemical substances to insect cells in vivo or in vitro. The mechanisms of necrosis, apoptosis or autophagy induced by synthetic or biogenic pesticides and virus infections have been systematically illuminated in many important models, including S2, BmN, SL-1, Sf21 and Sf9 cell lines...
September 2016: Pesticide Biochemistry and Physiology
Rui Guo, Guangli Cao, Yuexiong Zhu, Dhiraj Kumar, Renyu Xue, Yahong Lu, Xiaolong Hu, Chengliang Gong
Bombyx mori bidensovirus (BmBDV) was previously termed as Bombyx mori densovirus type 2 and later it was reclassified in the new genus bidensovirus of the new family Bidnaviridae. The genome of BmBDV Zhenjiang isolate (BmBDV-Z) consists of two non-homologous single-stranded linear DNA molecules VD1 and VD2 which are encapsidated into separate virion. To investigate the infectivity of BmBDV DNA, recombinant plasmids pGEM-VD1 inserted with VD1 genome were transfected into the BmN cells of silkworm. Structural proteins of BmBDV were detected with Western blot and immunofluorescence assay, which indicates pGEM-VD1 replicated in the transfected BmN cells and viral proteins were also expressed...
October 2016: Current Microbiology
Isaac Chambers, Phu Truong, K James Kallail, William Palko
Acute myeloid leukemia (AML) is the most common leukemia in adults. In rare cases, bone marrow necrosis (BMN) and osteolytic lesions are presenting features of AML. The following case describes a patient with known polycythemia vera (PV) that presented with signs of multiple myeloma, including hypercalcemia, anemia, and lytic lesions of the thoracic spine and skull. Laboratory workup was not indicative of myeloma. A bone marrow biopsy was performed, which revealed extensive BMN and initial pathology was consistent with metastatic carcinoma...
2016: Curēus
Chen Zhang, Yongdi Wang, Qiang Fang, Minlin Xu, Mengyuan Lv, Jinxu Liao, Si Li, Zuoming Nie, Wenping Zhang
Thymosins have been highly conserved during evolution. These hormones exist in many animal species and play an essential role in many biological events. However, little is known regarding the physiological function of silkworm Bombyx mori thymosin (BmTHY). In this study, we investigated the expression pattern of BmTHY in a Bombyx mori larval ovarian cell line (BmN) challenged with Bombyx mori nuclear polyhydrosis virus (BmNPV) and the antiviral effect of recombinant BmTHY (rBmTHY) for Bombyx mori against BmNPV...
2016: Journal of Insect Science
Cui Zhao, Chen Zhang, Bin Chen, Yanghui Shi, Yanping Quan, Zuoming Nie, Yaozhou Zhang, Wei Yu
A DNA-binding protein (DBP) [GenBank accession number: M63416] of Bombyx mori nuclear polyhedrosis virus (BmNPV) has been reported to be a regulatory factor in BmNPV, but its detailed functions remain unknown. In order to study the regulatory mechanism of DBP on viral proliferation, genome replication, and gene transcription, a BmNPV dbp gene knockout virus dbp-ko-Bacmid was generated by the means of Red recombination system. In addition, dbp-repaired virus dbp-re-Bacmid was constructed by the means of the Bac to Bac system...
2016: PloS One
Bin-Bin Wang, Yi Xie, Fan-Chi Li, Min Ni, Kai-Zun Xu, Jiang-Hai Tian, Jing-Sheng Hu, Bin Xue, Wei-De Shen, Bing Li
The main mechanism of toxicity of organophosphate (OP) and carbamate (CB) insecticides is their irreversible binding and inhibition of acetylcholinestrase (AChE), encoded by ace1 (acetylcholinestrase gene 1), leading to eventual death of insects. Mutations in AChE may significantly reduce insects susceptibility to these pesticides. Bombyx mori is an important beneficial insect, and no OP- or CB-resistant strains have been generated. In this study, wild-type ace1 (wace1) and mutant ace1 (mace1) were introduced into BmN cells, confirmed by screening and identification...
October 2016: Archives of Insect Biochemistry and Physiology
Panpan Zhang, Di Miao, Yahui Zhang, Meizi Wang, Zhaoyang Hu, Peng Lü, Qin Yao
BACKGROUND: Bombyx mori bidensovirus (BmBDV), which belongs to the Bidnaviridae family established by the International Committee on Taxonomy of Viruses in 2011, was the first bidensovirus identified in insects. The structure of BmBDV is similar to that of parvoviruses, while its replication is similar to that of adenoviruses. Although BmBDV has the potential to be used as a tool in biological pest control and as an expression vector, virus rescue has been a bottleneck in the application of this virus...
2016: Virology Journal
Yong Zhou, Chengcheng Wu, Qing Sheng, Caiying Jiang, Qin Chen, Zhengbing Lv, Juming Yao, Zuoming Nie
Lysine acetylation (Kac) is a vital post-translational modification that plays an important role in many cellular processes in organisms. In the present study, the nutrient storage proteins in hemolymph were first found to be highly acetylated-particularly SP2 protein, which contains 20 potential Kac sites. Further results confirmed that lysine acetylation could stabilize and up-regulate the protein level of anti-apoptosis protein SP2, thereby improving the survival of H2O2-treated BmN cells and suppressing the apoptosis induced by H2O2...
August 2016: Journal of Insect Physiology
Tai-Hang Liu, Xiao-Long Dong, Cai-Xia Pan, Guo-Yu Du, Yun-Fei Wu, Ji-Gui Yang, Peng Chen, Cheng Lu, Min-Hui Pan
Atlastin is a member of the dynamin protein superfamily and it can mediate homotypic fusion of endoplasmic reticulum (ER) membranes, which is required for many biological processes. In this study, a new Atlastin homologous protein, BmAtlastin-n, was characterized in silkworms and was found to contain an N-terminal conserved GTPase domain and a coiled-coil middle domain. BmAtlastin-n is localized in the cytoplasm and enriched in silkworm midgut. Results also showed that overexpression of BmAtlastin-n in BmN-SWU1 cells could enhance resistance to BmNPV...
2016: Scientific Reports
Jingwen Yang, Zhangfei Shen, Xue Jiang, Huipeng Yang, Haishan Huang, Lili Jin, Yajie Chen, Liangen Shi, Naiming Zhou
Agonist-induced internalization plays a key role in the tight regulation of the extent and duration of G protein-coupled receptor signaling. Previously, we have shown that the Bombyx corazonin receptor (BmCrzR) activates both Gαq- and Gαs-dependent signaling cascades. However, the molecular mechanisms involved in the regulation of the internalization and desensitization of BmCrzR remain to be elucidated. Here, vectors for expressing BmCrzR fused with enhanced green fluorescent protein (EGFP) at the C-terminal end were used to further characterize BmCrzR internalization...
July 19, 2016: Biochemistry
M-M Ji, A-Q Liu, Y-H Sima, S-Q Xu
The pathway of communication between endocrine hormones and ribosome biogenesis critical for physiological adaptation is largely unknown. Nucleolar essential protein 1 (Nep1) is an essential gene for ribosome biogenesis and is functionally conserved in many in vertebrate and invertebrate species. In this study, we cloned Bombyx mori Nep1 (BmNep1) due to its high expression in silk glands of silkworms on day 3 of the fifth instar. We found that BmNep1 mRNA and protein levels were upregulated in silk glands during fourth-instar ecdysis and larval-pupal metamorphosis...
October 2016: Insect Molecular Biology
Xin Gao, Huadong Xu, Jing Shang, Lan Yuan, Yongming Zhang, Lele Wang, Wenxiao Zhang, Xianguo Luan, Guiping Hu, Hongqian Chu, Tong Zhu, Guang Jia
Black carbon and tropospheric ozone (O3 ), which are major air pollutants in China, are hazardous to humans following inhalation. Black carbon can be oxidized by O3 forming secondary particles of which the health effects are unknown. The present study utilized carbon black as a representative of black carbon to characterize the cytotoxicity induced by secondary particles in bronchial epithelial cells (16HBE) and C57BL/6J mice, and to investigate the implicated molecular pathways. Two types of carbon black including untreated carbon black (UCB) and ozonized carbon black (OCB) were presented...
June 14, 2016: Environmental Toxicology
La Wang, Juan Song, Xi-Yan Bao, Peng Chen, Hua-Shan Yi, Min-Hui Pan, Cheng Lu
The identification and analysis of the caspases is essential to research into apoptosis in lepidoptera insects. The domesticated silkworm, Bombyx mori, is the model system for lepidopterans. In this study, we cloned and characterized a B. mori Dredd gene, BmDredd, the proposed insect homologue of human caspase-8, which encoded a polypeptide of 543 amino acids. BmDredd possesses a long N-terminal prodomain, a p20 domain, and a p10 domain. When transiently expressed in Escherichia coli cells, BmDredd underwent spontaneous cleavage and exhibited high proteolytic activity for caspase-8 substrate but relatively low for caspase-3 or -9 substrate...
October 15, 2016: Gene
Gajula Gopinath, Kallare P Arunkumar, Kazuei Mita, Javaregowda Nagaraju
Deciphering the regulatory factors involved in Bombyx mori sex determination has been a puzzle, challenging researchers for nearly a century now. The pre-mRNA of B. mori doublesex (Bmdsx), a master regulator gene of sexual differentiation, is differentially spliced, producing Bmdsxm and Bmdsxf transcripts in males and females respectively. The putative proteins encoded by these differential transcripts orchestrate antagonistic functions, which lead to sexual differentiation. A recent study in B. mori illustrated the role of a W-derived fem piRNA in conferring femaleness...
August 2016: Insect Biochemistry and Molecular Biology
Chiaki Nagai-Okatani, Hiromichi Nagasawa, Shinji Nagata
Recently, we identified an orphan Bombyx mori neuropeptide G protein-coupled receptor (BNGR)-A24 as an ion transport peptide-like (ITPL) receptor. BNGR-A24 belongs to the same clade as BNGR-A32 and -A33, which were recently identified as natalisin receptors. Since these three BNGRs share high similarities with known receptors for tachykinin-related peptides (TRPs), we examined whether these BNGRs can function as physiological receptors for five endogenous B. mori TRPs (TK-1-5). In a heterologous expression system, BNGR-A24 acted as a receptor for all five TRPs...
2016: PloS One
Rui Guo, Guangli Cao, Yahong Lu, Renyu Xue, Dhiraj Kumar, Xiaolong Hu, Chengliang Gong
Nosema bombycis, a microsporidium, is a pathogen of pebrine disease of silkworms, and its genomic DNA sequences had been determined. Thus far, the research of gene functions of microsporidium including N. bombycis cannot be performed with gain/loss of function. In the present study, we targeted to construct transgenic N. bombycis. Therefore, hemocytes of the infected silkworm were transfected with a non-transposon vector pIZT/V5-His vector in vivo, and the blood, in which the hemocyte with green fluorescence could be observed, was added to the cultured BmN cells...
August 2016: Parasitology Research
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