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quorum sensing and resistence gene

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https://www.readbyqxmd.com/read/28818490/inhibition-of-quorum-sensing-controlled-biofilm-formation-in-pseudomonas-aeruginosa-by-quorum-sensing-inhibitors
#1
Kalaiarasan Ellappan, Kottha Thirumalaswamy, Belgode Narasimha Harish, Gnanasambandam Vasuki, Veeresh Kumar Sali, James John
Antimicrobial therapy against extensively drug-resistant (XDR) P. aeruginosa biofilms is less efficient compared to the treatment of equal bacterial counts of free-floating planktonic cells, which has become a serious threat in hospital environment. P. aeruginosa regulate their cooperative activities and physiological process through a cell to cell chemical communication process called Quorum sensing (QS). This attracted our interest to synthesize, and to chemically characterize two anti-QS compounds, N-(4-{4-fluoroanilno} butanoyl) -L-homoserine lactone (FABHL) and N-(4-{4-chlororoanilno} butanoyl) -L-homoserine lactone (CABHL) to inhibit biofilm formation via disabling the QS circuits...
August 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28810631/hyperoside-inhibits-biofilm-formation-of-pseudomonas-aeruginosa
#2
Yixuan Sun, Fengjun Sun, Wei Feng, Xuewen Qiu, Yao Liu, Bo Yang, Yongchuan Chen, Peiyuan Xia
Pseudomonas aeruginosa (P. aeruginosa) is a common pathogen in hospital-acquired infection and is readily able to form biofilms. Due to its high antibiotic resistance, traditional antibacterial treatments exert a limited effect on P. aeruginosa biofilm infections. It has been indicated that hyperoside inhibits P. aeruginosa PAO1 (PAO1) biofilm formation without affecting growth. Therefore, the current study examined the biofilm formation and quorum sensing (QS) system of PAO1 in the presence of hyperoside. Confocal laser scanning microscopy analysis demonstrated that hyperoside significantly inhibited biofilm formation...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28808129/gene-duplication-in-pseudomonas-aeruginosa-improves-growth-on-adenosine
#3
Jean-Paul Toussaint, Anna Farrell-Sherman, Tamar Perla Feldman, Nicole E Smalley, Amy L Schaefer, E Peter Greenberg, Ajai A Dandekar
The laboratory strain of Pseudomonas aeruginosa, PAO1, activates genes for catabolism of adenosine using quorum sensing (QS). However, this strain is not well-adapted for growth on adenosine, with doubling times greater than 40 h. We previously showed that when PAO1 is grown on adenosine and casein, variants emerge that grow rapidly on adenosine. To understand the mechanism by which this adaptation occurs, we performed whole-genome sequencing of five isolates evolved for rapid growth on adenosine. All five genomes had a gene duplication-amplification (GDA) event covering several genes, including the quorum-regulated nucleoside hydrolase, nuh, and an adenine deaminase encoded by PA0148...
August 14, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28802267/development-of-a-transient-muts-based-hypermutation-system-for-adaptive-evolution-of-lactobacillus-casei-to-low-ph
#4
Tom J Overbeck, Dennis L Welker, Joanne E Hughes, James L Steele, Jeff R Broadbent
This study explored transient inactivation of the gene encoding the DNA mismatch repair enzyme MutS as a tool for adaptive evolution of Lactobacillus casei MutS deletion derivatives of L. casei 12A and ATCC 334 were constructed and subject to a 100-day adaptive evolution process to increase lactic acid resistance at low pH. Wild-type parental strains were also subject to this treatment. At the end of the process, the ΔmutS lesion was repaired in representative L. casei 12A and ATCC 334 ΔmutS isolates. Growth studies in broth at pH 4...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28798903/anti-quorum-sensing-and-anti-biofilm-activity-of-delftia-tsuruhatensis-extract-by-attenuating-the-quorum-sensing-controlled-virulence-factor-production-in-pseudomonas-aeruginosa
#5
Vijay K Singh, Avinash Mishra, Bhavanath Jha
Multidrug-resistance bacteria commonly use cell-to-cell communication that leads to biofilm formation as one of the mechanisms for developing resistance. Quorum sensing inhibition (QSI) is an effective approach for the prevention of biofilm formation. A Gram-negative bacterium, Delftia tsuruhatensis SJ01, was isolated from the rhizosphere of a species of sedge (Cyperus laevigatus) grown along the coastal-saline area. The isolate SJ01 culture and bacterial crude extract showed QSI activity in the biosensor plate containing the reference strain Chromobacterium violaceum CV026...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28792229/eugenol-exhibits-anti-virulence-properties-by-competitively-binding-to-quorum-sensing-receptors
#6
Prasanth Rathinam, H S Vijay Kumar, Pragasam Viswanathan
The primary objective of this study was to ascertain the anti-biofilm and anti-virulence properties of sub-minimum inhibitory concentration (MIC) levels of eugenol against the standard strain PAO1 and two multi-drug resistant P. aeruginosa clinical isolates utilizing quorum sensing inhibition (QSI). Eugenol at 400 μM significantly reduced biofilm formation on urinary catheters and the virulence factors (VF) including extracellular polysaccharides, rhamnolipid, elastase, protease, pyocyanin, and pyoverdine (p < 0...
August 9, 2017: Biofouling
https://www.readbyqxmd.com/read/28755299/interkingdom-signaling-in-plant-microbe-interactions
#7
REVIEW
Jinhong Kan, Rongxiang Fang, Yantao Jia
The widespread communications between prokaryotes and eukaryotes via signaling molecules are believed to affect gene expression in both partners. During the communication process, the contacted organisms produce and release small molecules that establish communication channels between two kingdoms-this procedure is known as interkingdom signaling. Interkingdom communications are widespread between pathogenic or beneficial bacteria and their host plants, with diversified outcomes depending on the specific chemical-triggered signaling pathways...
July 27, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28731269/biofilm-formation-and-antibiotic-resistance-in-klebsiella-pneumoniae-urinary-strains
#8
Claudia Vuotto, Francesca Longo, Chiara Pascolini, Gianfranco Donelli, Maria Pia Balice, Maria Francesca Libori, Valentina Tiracchia, Antonino Salvia, Pietro Emanuele Varaldo
AIMS: Multi-drug resistant Klebsiella pneumoniae has become a relevant healthcare-associated pathogen. Capsule, type 1 and 3 fimbriae (mrkA gene), type 2 quorum-sensing system (luxS), synthesis of D-galactan I (wbbM), LPS transport (wzm) and poly-beta-1,6-N-acetyl-D-glucosamine (pgaA) seem involved in K. pneumoniae biofilm. Non-enzymatic antibiotic resistance is related to non-expression or mutation of porins (OmpK35 and OmpK36), and efflux pump (acrB) over-expression. The aim of this study was to analyse some virulence factors of K...
July 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28729210/deficiency-of-quorum-sensing-system-inhibits-the-resistance-selection-of-pseudomonas-aeruginosa-to-ciprofloxacin-and-levofloxacin-in-vitro
#9
Yating Gao, Jinju Duan, Xin Geng, Zhiqi Zhang, Ruiqin Zhang, Xiaoxia Li, Shuyun Wang, Jianbang Kang, Donghong Yin, Yan Song
OBJECTIVES: The objective was to investigate how deficient of quorum sensing system (QS) affects minimal inhibitory concentrations (MICs) of Pseudomonas aeruginosa strains to ciprofloxacin (CIP) and levofloxacin (LEV) in vitro. METHODS: Collected the clinical Pseudomonas aeruginosa isolates. Screened the strains deficient of QS genes by the PCR agarose electrophoresis, and then randomly picked out normal strains with the same number of deficient ones. All strains incubated on the different CIP-concentration Mueller-Hinton agars for five days, the different concentrations of CIP respectively refered to 0...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28720734/lack-of-acrb-efflux-function-confers-loss-of-virulence-on-salmonella-enterica-serovar-typhimurium
#10
Xuan Wang-Kan, Jessica M A Blair, Barbara Chirullo, Jonathan Betts, Roberto M La Ragione, Alasdair Ivens, Vito Ricci, Timothy J Opperman, Laura J V Piddock
AcrAB-TolC is the paradigm resistance-nodulation-division (RND) multidrug resistance efflux system in Gram-negative bacteria, with AcrB being the pump protein in this complex. We constructed a nonfunctional AcrB mutant by replacing D408, a highly conserved residue essential for proton translocation. Western blotting confirmed that the AcrB D408A mutant had the same native level of expression of AcrB as the parental strain. The mutant had no growth deficiencies in rich or minimal medium. However, compared with wild-type SL1344, the mutant had increased accumulation of Hoechst 33342 dye and decreased efflux of ethidium bromide and was multidrug hypersusceptible...
July 18, 2017: MBio
https://www.readbyqxmd.com/read/28714256/a1s_2811-a-chea-y-like-hybrid-two-component-regulator-from-acinetobacter%C3%A2-baumannii-atcc17978-is-involved-in-surface-motility-and-biofilm-formation-in-this-bacterium
#11
Rong Chen, Ruichen Lv, Lisheng Xiao, Min Wang, Zongmin Du, Yafang Tan, Yujun Cui, Yanfeng Yan, Yanping Luo, Ruifu Yang, Yajun Song
Two-component systems in Acinetobacter baumannii are associated with its virulence, drug resistance, motility, biofilm formation, and other characteristics. In this study, we used RecAb , a genetic engineering method, to investigate the function of A1S_2811 in A. baumannii strain ATCC17978. A1S_2811, a hypothetical hybrid sensor histidine kinase/response regulator, has four histidine-containing phosphotransfer domains, a CheA-like regulatory domain, and a CheY-like receiver domain at its C terminus. Compared with the ATCC17978 strain, both surface motility and biofilm formation at the gas-liquid interface decreased significantly in the A1S_2811 knock-out strain...
July 17, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28650325/evaluation-of-three-plant-extracts-against-biofilm-formation-and-expression-of-quorum-sensing-regulated-virulence-factors-in-pseudomonas-aeruginosa
#12
Vajihe Karbasizade, Parichehr Dehghan, Maryam Mohammadi Sichani, Kahin Shahanipoor, Reyhaneh Jafari, Rozita Yousefian
Following the increasing antibiotic resistance of pathogenic bacteria, the use of medicinal herbs as antibacterial agents has attracted growing attention. Pseudomonas aeruginosa is a human opportunistic pathogen that uses quorum sensing for regulating virulence gene expression (pyocyanin, protease, and elastase production and biofilm formation). This study examined the anti-quorum sensing activity of Quercus infectoria, Zataria multiflora and Trachyspermum copticum extracts on standard P. aeruginosa strain...
March 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28605194/discovery-of-two-bacterial-nitric-oxide-responsive-proteins-and-their-roles-in-bacterial-biofilm-regulation
#13
Sajjad Hossain, Lisa-Marie Nisbett, Elizabeth M Boon
Bacterial biofilms form when bacteria adhere to a surface and produce an exopolysaccharide matrix ( Costerton Science 1999 , 284 , 1318 ; Davies Science 1998 , 280 , 295 ; Flemming Nat. Rev. Microbiol. 2010 , 8 , 623 ). Because biofilms are resistant to antibiotics, they are problematic in many aspects of human health and welfare, causing, for instance, persistent fouling of medical implants such as catheters and artificial joints ( Brunetto Chimia 2008 , 62 , 249 ). They are responsible for chronic infections in the lungs of cystic fibrosis patients and in open wounds, such as those associated with burns and diabetes...
June 12, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28588273/myricetin-protects-galleria-mellonella-against-staphylococcus-aureus-infection-and-inhibits-multiple-virulence-factors
#14
L N Silva, G C A Da Hora, T A Soares, M S Bojer, H Ingmer, A J Macedo, D S Trentin
Staphylococcus aureus is an opportunistic pathogen related to a variety of life-threatening infections but for which antimicrobial resistance is liming the treatment options. We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. Myricetin affects both surface proteins and secreted proteins which indicate that its action is unrelated to inhibition of the agr quorum sensing system...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28487993/acinetobacter%C3%A2-baumannii-quorum-sensing-signalling-molecule-induces-the-expression-of-drug-resistance-genes
#15
Yi Dou, Fei Song, Feng Guo, Zengding Zhou, Cailian Zhu, Jun Xiang, Jingning Huan
Quorum-sensing signalling molecules such as N‑acyl homoserine lactones (AHLs) enable certain Gram‑negative bacteria to respond to environmental changes through behaviours, such as biofilm formation and flagellar movement. The present study aimed to identify Acinetobacter baumannii AHLs and assess their influence on antibiotic resistance. A clinical isolate of A. baumannii strain S (AbS) was collected from the wound of a burn patient and high‑performance liquid chromatography and tandem quadrupole or quadrupole time‑of‑flight high‑resolution mass spectrometry was used to identify AbS AHLs...
June 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28455333/a-novel-quorum-quenching-n-acylhomoserine-lactone-acylase-from-acidovorax-sp-strain-mr-s7-mediates-antibiotic-resistance
#16
Hiroyuki Kusada, Hideyuki Tamaki, Yoichi Kamagata, Satoshi Hanada, Nobutada Kimura
N-Acylhomoserine lactone acylase (AHL acylase) is a well-known enzyme responsible for disrupting cell-cell communication (quorum sensing) in bacteria. Here, we isolated and characterized a novel and unique AHL acylase (designated MacQ) from a multidrug-resistant bacterium, Acidovorax sp. strain MR-S7. The purified MacQ protein heterologously expressed in Escherichia coli degraded a wide variety of AHLs, ranging from C6 to C14 side chains with or without 3-oxo substitutions. We also observed that AHL-mediated virulence factor production in a plant pathogen, Pectobacterium carotovorum, was dramatically attenuated by coculture with MacQ-overexpressing Escherichia coli, whereas E...
July 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28453568/baicalin-inhibits-biofilm-formation-attenuates-the-quorum-sensing-controlled-virulence-and-enhances-pseudomonas-aeruginosa-clearance-in-a-mouse-peritoneal-implant-infection-model
#17
Jing Luo, Biying Dong, Ke Wang, Shuangqi Cai, Tangjuan Liu, Xiaojing Cheng, Danqing Lei, Yanling Chen, Yanan Li, Jinliang Kong, Yiqiang Chen
The quorum sensing (QS) circuit plays a role in the precise regulation of genes controlling virulence factors and biofilm formation in Pseudomonas aeruginosa. QS-controlled biofilm formation by Pseudomonas aeruginosa in clinical settings has remained controversial due to emerging drug resistance; therefore, screening diverse compounds for anti-biofilm or anti-QS activities is important. This study demonstrates the ability of sub-minimum inhibitory concentrations (sub-MICs) of baicalin, an active natural compound extracted from the traditional Chinese medicinal Scutellaria baicalensis, to inhibit the formation of Pseudomonas aeruginosa biofilms and enhance the bactericidal effects of various conventional antibiotics in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28446917/shoot-the-message-not-the-messenger-combating-pathogenic-virulence-in-plants-by-inhibiting-quorum-sensing-mediated-signaling-molecules
#18
REVIEW
Ganesh Alagarasan, Kumar S Aswathy
Immunity, virulence, biofilm formation, and survival in the host environment are regulated by the versatile nature of density dependent microbial cell signaling, also called quorum sensing (QS). The QS molecules can associate with host plant tissues and, at times, cause a change in its gene expression at the downstream level through inter-kingdom cross talking. Progress in controlling QS through fungicide/bactericide in pathogenic microscopic organisms has lead to a rise of antibiotic resistance pathogens. Here, we review the application of selective quorum quenching (QQ) endophytes to control phytopathogens that are shared by most, if not all, terrestrial plant species as well as aquatic plants...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28442545/peroxisome-proliferator-activated-receptor-%C3%AE-agonists-attenuate-biofilm-formation-by-pseudomonas-aeruginosa
#19
Brahmchetna Bedi, Nicholas M Maurice, Vincent T Ciavatta, K Sabrina Lynn, Zhihong Yuan, Samuel A Molina, Myungsoo Joo, William R Tyor, Joanna B Goldberg, Michael Koval, C Michael Hart, Ruxana T Sadikot
Pseudomonas aeruginosa is a significant contributor to recalcitrant multidrug-resistant infections, especially in immunocompromised and hospitalized patients. The pathogenic profile of P. aeruginosa is related to its ability to secrete a variety of virulence factors and to promote biofilm formation. Quorum sensing (QS) is a mechanism wherein P. aeruginosa secretes small diffusible molecules, specifically acyl homo serine lactones, such as N-(3-oxo-dodecanoyl)-l-homoserine lactone (3O-C12-HSL), that promote biofilm formation and virulence via interbacterial communication...
August 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28438469/regulation-of-conjugative-transfer-of-plasmids-and-integrative-conjugative-elements
#20
REVIEW
Luis Alfredo Bañuelos-Vazquez, Gonzalo Torres Tejerizo, Susana Brom
Horizontal gene transfer has been recognized as one of the principal contributors to bacterial evolution and diversification. One of the mechanisms involved in this process is conjugative transfer of plasmids and Integrative Conjugative Elements (ICEs). Plasmids and ICEs often encode traits beneficial for bacterial survival in specific environments, or for the establishment of symbiosis or pathogenesis, in addition to genes allowing conjugative transfer. In this review, we analyze the mechanisms that regulate the expression of conjugative transfer genes...
April 22, 2017: Plasmid
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