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quorum sensing and resistence gene

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https://www.readbyqxmd.com/read/29229911/comparative-genome-analysis-of-the-vineyard-weed-endophyte-pseudomonas-viridiflava-cdrtc14-showing-selective-herbicidal-activity
#1
Abdul Samad, Livio Antonielli, Angela Sessitsch, Stéphane Compant, Friederike Trognitz
Microbes produce a variety of secondary metabolites to be explored for herbicidal activities. We investigated an endophyte Pseudomonas viridiflava CDRTc14, which impacted growth of its host Lepidium draba L., to better understand the possible genetic determinants for herbicidal and host-interaction traits. Inoculation tests with a variety of target plants revealed that CDRTc14 shows plant-specific effects ranging from beneficial to negative. Its herbicidal effect appeared to be dose-dependent and resembled phenotypically the germination arrest factor of Pseudomonas fluorescens WH6...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29225430/insights-from-the-molecular-dynamics-simulation-of-bcsd-subunit-from-k-xylinus
#2
Simranjit Kaur, Mahesh Kulharia
Biofilms are bacteria living in micro-colonies with a protective coating in sessile form. The biofilm protects bacteria from harsh surroundings as well as help in antibiotics resistance using a semi-fluid substance. Cellulose is the major component of biofilm, which provides the sticky appearance to bacteria for attaching to the substratum. The bacteria communicate in biofilm with the help of quorum sensing hormones Acylated Homoserine Lactones (AHL's). In Komagataeibacter xylinus the four genes Bcs A, Bcs B, Bcs C, Bcs D are associated with cellulose biosynthesis...
2017: Bioinformation
https://www.readbyqxmd.com/read/29215165/transcriptional-response-of-nautella-italica-r11-towards-its-macroalgal-host-uncovers-new-mechanisms-of-host-pathogen-interaction
#3
Jennifer Hudson, Melissa Gardiner, Nandan Deshpande, Suhelen Egan
Macroalgae (seaweeds) are essential for the functioning of temperate marine ecosystems, but there is increasing evidence to suggest that their survival is under threat from anthropogenic stressors and disease. Nautella italica R11 is recognised as an etiological agent of bleaching disease in the red alga, Delisea pulchra. Yet, there is a lack of knowledge surrounding the molecular mechanisms involved in this model host-pathogen interaction. Here we report that mutations in the gene encoding for a LuxR-type quorum sensing transcriptional regulator, RaiR, render N...
December 7, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/29203527/a-novel-chemical-inducer-of-streptococcus-quorum-sensing-acts-by-inhibiting-the-pheromone-degrading-endopeptidase-pepo
#4
Tiara G Pérez Morales, Kiira Ratia, Duo-Sheng Wang, Artemis Gogos, Tom G Driver, Michael J Federle
Bacteria produce chemical signals (pheromones) to coordinate behaviors across a population in a process termed quorum sensing (QS). QS systems comprising peptide pheromones and their corresponding Rgg receptors are widespread among Firmicutes and may be useful targets for manipulating microbial behaviors, like suppressing virulence. The Rgg2/3 QS circuit of the human pathogen Streptococcus pyogenes controls genes affecting resistance to host lysozyme in response to short hydrophobic pheromones (SHPs). Considering that artificial activation of a QS pathway may be as useful in the objective of manipulating bacteria as inhibiting it, we sought to identify small-molecule inducers of the Rgg2/3 QS system...
December 4, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29178590/meloxicam-inhibits-biofilm-formation-and-enhances-antimicrobial-agents-efficacy-by-pseudomonas-aeruginosa
#5
Pengfei She, Yangxia Wang, Zhen Luo, Lihua Chen, Ruichen Tan, Yanle Wang, Yong Wu
Microbial biofilms are communities of surface-adhered cells enclosed in a matrix of extracellular polymeric substances. Bacterial cells in biofilm are 10~1,000-fold more resistant to antimicrobials than the planktonic cells. Burgeoning antibiotic resistance in Pseudomonas aeruginosa biofilm has necessitated the development of antimicrobial agents. Here, we have investigated the antibiofilm effect of meloxicam against P. aeruginosaPAO1 and its potential mechanisms. Further, we have explored whether meloxicam could enhance the susceptibility of bacterial biofilms to treatment with conventional antimicrobials...
November 27, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29150517/multi-omics-reveal-the-lifestyle-of-the-acidophilic-mineral-oxidizing-model-species-leptospirillum-ferriphilum-t
#6
Stephan Christel, Malte Herold, Sören Bellenberg, Mohamed El Hajjami, Antoine Buetti-Dinh, Igor V Pivkin, Wolfgang Sand, Paul Wilmes, Ansgar Poetsch, Mark Dopson
Leptospirillum ferriphilum plays a major role in acidic, metal rich environments where it represents one of the most prevalent iron oxidizers. These milieus include acid rock and mine drainage as well as biomining operations. Despite its perceived importance, no complete genome sequence of this model species' type strain is available, limiting the possibilities to investigate the strategies and adaptations Leptospirillum ferriphilum(T) applies to survive and compete in its niche. This study presents a complete, circular genome of Leptospirillum ferriphilum(T) DSM 14647 obtained by PacBio SMRT long read sequencing for use as a high quality reference...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29130185/identification-of-ai-2-quorum-sensing-inhibitors-in-vibrio-harveyi-through-structure-based-virtual-screening
#7
Tianyu Jiang, Peng Zhu, Lupei Du, Minyong Li
Quorum sensing (QS) is a cell-to-cell communication system that regulates gene expression as a result of the production and perception of signal molecules called autoinducers (AIs). AI-2 is a QS autoinducer produced by both Gram-negative and Gram-positive bacteria, in which it regulates intraspecies and interspecies communication. The identification of QS inhibitors is considered a promising strategy for the development of anti-virulence drugs with reduced selective pressure for resistance. Here we describe a high-throughput virtual screening approach to identify AI-2 quorum sensing inhibitors on the basis of Vibrio harveyi LuxPQ crystal structure...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130176/assessing-pseudomonas-aeruginosa-autoinducer-effects-on-mammalian-epithelial-cells
#8
Jake Everett, Rebecca Gabrilska, Kendra P Rumbaugh, Elena Vikström
The human mucosal environment in the gut is rich with interactions between microbiota and mammalian epithelia. Microbes such as the Gram-negative bacterium Pseudomonas aeruginosa may use quorum sensing to communicate with other microorganisms and mammalian cells to alter gene expression. Here, we present methodologies to evaluate the effects of P. aeruginosa N-(3-oxo-dodecanoyl)-L-homoserine lactone (3O-C12-HSL) on Caco-2 cell monolayers. First, we describe a method for assessing barrier function and permeability of epithelial cells when exposed to 3O-C12-HSL by measuring transepithelial electrical resistance (TER) and paracellular flow using fluorescently labeled dextran...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29099398/electric-field-based-dressing-disrupts-mixed-species-bacterial-biofilm-infection-and-restores-functional-wound-healing
#9
Kasturi Ganesh Barki, Amitava Das, Sriteja Dixith, Piya Das Ghatak, Shomita Mathew-Steiner, Elizabeth Schwab, Savita Khanna, Daniel J Wozniak, Sashwati Roy, Chandan K Sen
OBJECTIVE: This study was designed to employ electroceutical principles, as an alternative to pharmacological intervention, to manage wound biofilm infection. Mechanism of action of a United States Food and Drug Administration-cleared wireless electroceutical dressing (WED) was tested in an established porcine chronic wound polymicrobial biofilm infection model involving inoculation with Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii 19606. BACKGROUND: Bacterial biofilms represent a major wound complication...
November 2, 2017: Annals of Surgery
https://www.readbyqxmd.com/read/29095142/high-mortality-of-bloodstream-infection-outbreak-caused-by-carbapenem-resistant-p-aeruginosa-producing-spm-1-in-a-bone-marrow-transplant-unit
#10
Lucas Chaves, Lísia Moura Tomich, Matias Salomão, Gleice Cristina Leite, Jessica Ramos, Roberta Ruedas Martins, Camila Rizek, Patricia Neves, Marjorie Vieira Batista, Ulysses Amigo, Thais Guimaraes, Anna Sara Levin, Silvia Figueiredo Costa
PURPOSE: Carbapenem resistance in P. aeruginosa is increasing worldwide. In Brazil, SPM-1 is the main P. aeruginosa carbapenemase identified. Little is known about the virulence factor in SPM-1 clones.Methodolgy. We describe a carbapenem-resistant P. aeruginosa bloodstream infection (CRPa-BSI) outbreak in a bone marrow transplant Unit (BMT). Twenty-nine CRPa-BSI cases were compared to 58 controls. Microbiological characteristics of isolates, such as sensitivity, carbapenemase gene PCR for P...
November 2, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29050666/the-contribution-of-bacteriophages-to-the-biology-and-virulence-of-pathogenic-clostridia
#11
Louis-Charles Fortier
Bacteriophages are key players in the evolution of most bacteria. Temperate phages have been associated with virulence of some of the deadliest pathogenic bacteria. Among the most notorious cases, the genes encoding the botulinum neurotoxin produced by Clostridium botulinum types C and D and the α-toxin (TcnA) produced by Clostridium novyi are both encoded within prophage genomes. Clostridium difficile is another important human pathogen and the recent identification of a complete binary toxin locus (CdtLoc) carried on a C...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/29042302/attenuation-of-pseudomonas-aeruginosa-quorum-sensing-virulence-and-biofilm-formation-by-extracts-of-andrographis-paniculata
#12
Malabika Banerjee, Soumitra Moulick, Kunal Kumar Bhattacharya, Debaprasad Parai, Subrata Chattopadhyay, Samir Kumar Mukherjee
Quorum-sensing (QS) is known to play an essential role in regulation of virulence factors and toxins during Pseudomonas aeruginosa infection which may frequently cause antibiotic resistance and hostile outcomes of inflammatory injury. Therefore, it is an urgent need to search for a novel agent with low risk of resistance development that can target QS and inflammatory damage prevention as well. Andrographis paniculata, a herbaceous plant under the family Acanthaceae, native to Asian countries and also cultivated in Scandinavia and some parts of Europe, has a strong traditional usage with its known antibacterial, anti-inflammatory, antipyretic, antiviral and antioxidant properties...
October 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29038255/studies-of-pseudomonas-aeruginosa-mutants-indicate-pyoverdine-as-the-central-factor-in-inhibition-of-aspergillus-fumigatus-biofilm
#13
Gabriele Sass, Hasan Nazik, John Penner, Hemi Shah, Shajia Rahman Ansari, Karl V Clemons, Marie-Christine Groleau, Anna-Maria Dietl, Paolo Visca, Hubertus Haas, Eric Déziel, David A Stevens
Pseudomonas aeruginosa (Pa) and Aspergillus fumigatus (Af) are common opportunistic bacterial and fungal pathogens, respectively. They often co-exist in airways of immunocompromised patients and individuals with cystic fibrosis, where they form biofilms and cause acute and chronic illnesses. Hence the interactions between them have long been of interest, and it is known that Pa can inhibit Af in vitro We have approached the definition of the inhibitory Pa molecules by studying 24 Pa mutants, with various virulence genes deleted, for their ability to inhibit Af biofilms...
October 16, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29022382/opposing-effect-of-lactobacillus-on-in-vitro-klebsiella-pneumoniae-in-biofilm-and-in-an-in-vivo-intestinal-colonisation-model
#14
R Lagrafeuille, S Miquel, D Balestrino, M Vareille-Delarbre, F Chain, P Langella, C Forestier
Beneficial bacteria represent potential sources of therapy, particularly in the battle against antibiotic-resistant pathogens. The Gram-negative bacillus Klebsiella pneumoniae is not only a paradigm of multi-resistant opportunistic pathogen, but it is also able to colonise the human intestine and displays a high capacity to form biofilm. In this study, the anti-biofilm activity of 140 neutralised Lactobacillus supernatants was assessed against K. pneumoniae. Among the 13 strains whose supernatant significantly impaired biofilm formation, Lactobacillus plantarum CIRM653 was selected because it was also able to impair K...
October 12, 2017: Beneficial Microbes
https://www.readbyqxmd.com/read/28993333/antibiotics-promote-escherichia-coli-pseudomonas-aeruginosa-conjugation-through-inhibiting-quorum-sensing
#15
Yang Lu, Jianming Zeng, Linjing Wang, Kai Lan, E Shunmei, Lina Wang, Qian Xiao, Qiang Luo, Xianzhang Huang, Bin Huang, Cha Chen
The effect of antibiotics on horizontal gene transfer is controversial and the underlying mechanism remains poorly understood. Here, using E. coli SM10λπ which carries a chromosomally integrated RP4 plasmid as donor strain, we investigated the effect of antibiotics on conjugational transfer of a mobilizable gentamicin (Gm) resistance plasmid. The results showed that exposure to gentamicin that may restrict the survival of recipient cells significantly enhanced SM10λπ-P. aeruginosa (PAO1) conjugation, which was attenuated by deficiency of lasI/rhlI, genes associated with generation of the quorum sensing signals N-acyl homoserine lactones (AHLs) in PAO1, or deletion of the AHLs receptor SdiA in SM10λπ...
October 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28983655/mechanistic-understanding-of-phenyllactic-acid-mediated-inhibition-of-quorum-sensing-and-biofilm-development-in-pseudomonas-aeruginosa
#16
Maitrayee Chatterjee, Sharon D'Morris, Vinod Paul, Sruthi Warrier, Anil Kumar Vasudevan, Muralidharan Vanuopadath, Sudarslal Sadasivan Nair, Bindhu Paul-Prasanth, C Gopi Mohan, Raja Biswas
Pseudomonas aeruginosa depends on its quorum sensing (QS) system for its virulence factors' production and biofilm formation. Biofilms of P. aeruginosa on the surface of indwelling catheters are often resistant to antibiotic therapy. Alternative approaches that employ QS inhibitors alone or in combination with antibiotics are being developed to tackle P. aeruginosa infections. Here, we have studied the mechanism of action of 3-Phenyllactic acid (PLA), a QS inhibitory compound produced by Lactobacillus species, against P...
October 5, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28970736/comment-on-effect-of-biofilm-formation-by-clinical-isolates-of-helicobacter-pylori-on-the-efflux-mediated-resistance-to-commonly-used-antibiotics
#17
COMMENT
Evangelos I Kazakos, Nick Dorrell, Stergios A Polyzos, Georgia Deretzi, Jannis Kountouras
Attaran et al([1]) have recently shown that decreased susceptibility of established Helicobacter pylori (H. pylori) biofilms to specific antibiotics, was associated with the overtly enhanced transcription of two efflux pump genes, hp1165 and hefA, involved in specific resistance to tetracycline and multiple antibiotics, respectively. Apart from antibiotic exposure, secretion of multiple antimicrobial peptides, such as human β-defensins (hβDs), by the gastric epithelium upon Hp challenge, may act as early triggering events that positively impact biofilm formation and thus, antibiotic resistance...
September 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28934400/genomic-characterisation-of-environmental-pseudomonas-aeruginosa-isolated-from-dental-unit-waterlines-revealed-the-insertion-sequence-ispa11-as-a-chaotropic-element
#18
Antony T Vincent, Luca Freschi, Julie Jeukens, Irena Kukavica-Ibrulj, Jean-Guillaume Emond-Rheault, Annie Leduc, Brian Boyle, Fabrice Jean-Pierre, Marie-Christine Groleau, Eric Déziel, Jean Barbeau, Steve J Charette, Roger C Levesque
The bacterium Pseudomonas aeruginosa is well known to have a remarkable adaptive capacity allowing it to colonise many environments. A recent study on environmental isolates from dental unit waterlines (DUWLs) hinted at a genetic clustering into two groups. Isolates from one of these groups, named cluster III, were shown to have unusual phenotypes for environmental isolates, such as an increased biofilm production. To have a better ecological view, more specifically on isolates from cluster III, the complete genomes of 39 isolates including 16 from DUWLs were sequenced...
September 1, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28930136/methanol-extract-from-anogeissus-leiocarpus-dc-guill-et-perr-combretaceae-stem-bark-quenches-the-quorum-sensing-of-pseudomonas-aeruginosa-pao1
#19
Vincent Ouedraogo, Martin Kiendrebeogo
Background: Due to its extensive arsenal of virulence factors and inherent resistance to antibiotics, Pseudomonas aeruginosa is a threat particularly in immunocompromised patients. Considering the central role of quorum sensing in the production of virulence factors, inhibition of bacterial communication mechanism constitute an opportunity to attenuate pathogenicity of bacteria resistant to available antibiotics. Our study aimed to assess the anti-quorum sensing activity of Anogeissus leiocarpus, traditionally used in Burkina Faso, for the treatment of infected burn wounds...
October 6, 2016: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28927382/a-rapid-seamless-method-for-gene-knockout-in-pseudomonas-aeruginosa
#20
Weiliang Huang, Angela Wilks
BACKGROUND: Pseudomonas aeruginosa is a model organism for the study of quorum sensing, biofilm formation, and also leading cause of nosocomial infections in immune compromised patients. As such P. aeruginosa is one of the most well studied organisms in terms of its genetics. However, the construction of gene deletions and replacements in Pseudomonas aeruginosa is relatively time-consuming, requiring multiple steps including suicide vector construction, conjugation, inactivation with insertion of antibiotic resistance cassettes and allelic exchange...
September 19, 2017: BMC Microbiology
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