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https://www.readbyqxmd.com/read/29887014/immobilized-cutinases-preparation-solvent-tolerance-and-thermal-stability
#1
An Su, Abhijit Shirke, Joshua Baik, Yi Zou, Richard Gross
Developing active immobilized enzymes and characterization of their use conditions is critically important prior to initiating studies of enzyme selectivity and substrate specificity in organic media. To this end, physical immobilization by hydrophobic interactions was performed with three well-characterized cutinases (Aspergillus oryzae Cutinase (AoC), Humicola insolens Cutinase (HiC), and Thielavia terrestris Cutinase (TtC)) using Lewatit VP OC 1600 as the macroporous support. We found that immobilization yields >98% were achieved for all three cutinases under the following immobilization conditions: 100 mg/g loading ratio, immobilization buffers of 100 mM phosphate pH 8 (AoC and HiC) and 100 mM acetate pH 5 (TtC), mixing at 150 rpm and 30 °C for 24 h...
September 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29881816/hepatic-iron-concentration-correlates-with-insulin-sensitivity-in-nonalcoholic-fatty-liver-disease
#2
Laurence Britton, Kim Bridle, Janske Reiling, Nishreen Santrampurwala, Leesa Wockner, Helena Ching, Katherine Stuart, V Nathan Subramaniam, Gary Jeffrey, Tim St Pierre, Michael House, Joel Gummer, Robert Trengove, John Olynyk, Darrell Crawford, Leon Adams
Rodent and cell-culture models support a role for iron-related adipokine dysregulation and insulin resistance in the pathogenesis of nonalcoholic fatty liver disease (NAFLD); however, substantial human data are lacking. We examined the relationship between measures of iron status, adipokines, and insulin resistance in patients with NAFLD in the presence and absence of venesection. This study forms part of the Impact of Iron on Insulin Resistance and Liver Histology in Nonalcoholic Steatohepatitis (IIRON2) study, a prospective randomized controlled trial of venesection for adults with NAFLD...
June 2018: Hepatology Communications
https://www.readbyqxmd.com/read/29879430/mef2-and-the-tumorigenic-process-hic-sunt-leones
#3
REVIEW
Eros Di Giorgio, Wayne W Hancock, Claudio Brancolini
While MEF2 transcription factors are well known to cooperate in orchestrating cell fate and adaptive responses during development and adult life, additional studies over the last decade have identified a wide spectrum of genetic alterations of MEF2 in different cancers. The consequences of these alterations, including triggering and maintaining the tumorigenic process, are not entirely clear. A deeper knowledge of the molecular pathways that regulate MEF2 expression and function, as well as the nature and consequences of MEF2 mutations are necessary to fully understand the many roles of MEF2 in malignant cells...
June 4, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29866075/hepatic-arterial-infusion-chemotherapy-followed-by-sorafenib-in-patients-with-advanced-hepatocellular-carcinoma-hics-55-an-open-label-non-comparative-phase-ii-trial
#4
Masahiro Hatooka, Tomokazu Kawaoka, Hiroshi Aikata, Yuki Inagaki, Kei Morio, Takashi Nakahara, Eisuke Murakami, Masataka Tsuge, Akira Hiramatsu, Michio Imamura, Yoshiiku Kawakami, Kazuo Awai, Keiichi Masaki, Koji Waki, Hirotaka Kohno, Hiroshi Kohno, Takashi Moriya, Yuko Nagaoki, Toru Tamura, Hajime Amano, Yoshio Katamura, Kazuaki Chayama
BACKGROUND: In patients with advanced hepatocellular carcinoma (HCC), evidence is unclear as to whether hepatic arterial infusion chemotherapy (HAIC) or sorafenib is superior. We performed a prospective, open-label, non-comparative phase II study to assess survival with HAIC or HAIC converted to sorafenib. METHODS: Fifty-five patients were prospectively enrolled. Patients received HAIC as a second course if they had complete response, partial response, or stable disease (SD) with an alpha fetoprotein (AFP) ratio < 1 or a des-γ-carboxy prothrombin (DCP) ratio < 1...
June 4, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29864957/tug1-alleviates-hypoxia-injury-by-targeting-mir-124-in-h9c2-cells
#5
Nianxin Jiang, Jingwen Xia, Bing Jiang, Yinghui Xu, Yansong Li
TUG1 has been reported to play an important role in various cancer types. However, the study about the function of lncRNA TUG1 in myocardial infarction is limited. This study was aimed to investigate the role of TUG1 in H9c2 cell injury induced by hypoxia and explore its possible molecular mechanism. The proliferation assay, migration assay, invasion assay, and apoptosis assay were performed. RT-PCR was used to determine the relative RNA expression of TUG1, miR-124 and Hic-5. Western blotting was used to detect the expression levels of apoptotic proteins, Hic-5, Sp1, and Survivin...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29860213/broad-application-and-optimization-of-a-single-wash-step-for-integrated-endotoxin-depletion-during-protein-purification
#6
David Koziel, Uwe Michaelis, Tobias Kruse
Endotoxins contaminate proteins that are produced in E. coli. High levels of endotoxins can influence cellular assays and cause severe adverse effects when administered to humans. Thus, endotoxin removal is important in protein purification for academic research and in GMP manufacturing of biopharmaceuticals. Several methods exist to remove endotoxin, but often require additional downstream-processing steps, decrease protein yield and are costly. These disadvantages can be avoided by using an integrated endotoxin depletion (iED) wash-step that utilizes Triton X-114 (TX114)...
May 21, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29846060/online-hydrophobic-interaction-chromatography-mass-spectrometry-for-the-analysis-of-intact-monoclonal-antibodies
#7
Bifan Chen, Ziqing Lin, Andrew J Alpert, Cexiong Fu, Qunying Zhang, Wayne A Pritts, Ying Ge
Therapeutic monoclonal antibodies (mAbs) are an important class of drugs for a wide spectrum of human diseases. Liquid chromatography (LC) coupled to mass spectrometry (MS) is one of techniques in the forefront for comprehensive characterization of analytical attributes of mAbs. Among various protein chromatography modes, hydrophobic interaction chromatography (HIC) is a popular offline non-denaturing separation technique utilized to purify and analyze mAbs, typically with the use of non-MS-compatible mobile phases...
May 30, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29791512/cpr-ir-is-an-insulin-resistance-index-that-is-minimally-affected-by-hepatic-insulin-clearance-a-preliminary-research
#8
Tsuyoshi Okura, Risa Nakamura, Yohei Fujioka, Sonoko Kawamoto-Kitao, Yuichi Ito, Kazuhisa Matsumoto, Kyoko Shoji, Keisuke Sumi, Kazuhiko Matsuzawa, Shoichiro Izawa, Etsuko Ueta, Masahiko Kato, Takeshi Imamura, Shin-Ichi Taniguchi, Kazuhiro Yamamoto
BACKGROUND: Increased hepatic insulin clearance (HIC) is important in the pathophysiology of type 2 diabetes mellitus (T2DM). The aim of this study is to analyze an effective insulin resistance (IR) index that is minimally affected by HIC. METHODS: Our study involved 20 participants with T2DM and 21 healthy participants without diabetes (Non-DM). Participants underwent a meal tolerance test from which plasma glucose, insulin and serum C-peptide immunoreactivity (CPR) were measured, and HOMA-IR and HIC were calculated...
2018: PloS One
https://www.readbyqxmd.com/read/29790950/the-research-data-management-platform-rdmp-a-novel-process-driven-open-source-tool-for-the-management-of-longitudinal-cohorts-of-clinical-data
#9
Thomas Nind, James Galloway, Gordon McAllister, Donald Scobbie, Wilfred Bonney, Christopher Hall, Leandro Tramma, Parminder Reel, Martin Groves, Philip Appleby, Alex Doney, Bruce Guthrie, Emily Jefferson
Background: The Health Informatics Centre (HIC) at the University of Dundee provides a service to securely host clinical datasets and extract relevant data for anonymised cohorts to researchers to enable them to answer key research questions. As is common in research using routine healthcare data, the service was historically delivered using ad-hoc processes resulting in the slow provision of data whose provenance was often hidden to the researchers using it. This paper describes the development and evaluation of the Research Data Management Platform (RDMP): an open source tool to load, manage, clean, and curate longitudinal healthcare data for research and provide reproducible and updateable datasets for defined cohorts to researchers...
May 22, 2018: GigaScience
https://www.readbyqxmd.com/read/29787622/mother-to-child-transmission-of-hiv-in-australia-and-other-high-income-countries-trends-in-perinatal-exposure-demography-and-uptake-of-prevention-strategies
#10
REVIEW
Kelly O'Donovan, Theophilus I Emeto
Virtual elimination of mother-to-child transmission (MTCT) of human immunodeficiency virus (HIV) is a global target. A review of the literature was conducted using medical databases and health department websites to examine the current trends related to perinatal HIV exposure and MTCT in Australia in comparison with other high-income countries (HICs). The review discusses the uptake of prevention strategies and barriers that impede MTCT prevention. The literature suggests an increase in the numbers of HIV-exposed deliveries, but a marked decline in the rates of MTCT within HICs...
May 22, 2018: Australian & New Zealand Journal of Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/29786021/developing-and-implementing-a-global-emergency-medicine-course-lessons-learned-from-rwanda
#11
Sojung Yi, Olivier Félix Umuhire, Doris Uwamahoro, Mindi Guptill, Giles N Cattermole
Background: There is a growing demand by medical trainees for meaningful, short-term global emergency medicine (EM) experiences. EM programs in high-income countries (HICs) have forged opportunities for their trainees to access this experience in low-and middle-income countries (LMICs). However, few programs in LMICs have created and managed such courses. As more LMICs establish EM programs, these settings are ideal for developing courses beneficial for all participants. We describe our experience of creating and implementing a short-term global EM course in Rwanda...
September 2017: Education for Health: Change in Training & Practice
https://www.readbyqxmd.com/read/29776618/sleep-health-epidemiology-in-low-and-middle-income-countries-a-systematic-review-and-meta-analysis-of-the-prevalence-of-poor-sleep-quality-and-sleep-duration
#12
REVIEW
Guido Simonelli, Nathaniel S Marshall, Antigone Grillakis, Christopher B Miller, Camilla M Hoyos, Nick Glozier
Sleep research has been dominated by high income countries (HIC). Sleep may be different in low and middle income countries (LMIC) due to cultural, demographic, geographical and health factors. We systematically reviewed the epidemiological literature reporting sleep parameters in the adult population in LMIC and meta-analyzed the prevalence of subjective poor sleep quality and sleep duration. We identified 45 publications; over 50% of which came from China and Brazil. Of the 45 identified studies, 32 contained data on sleep quality and 17 on self-reported sleep duration...
June 2018: Sleep Health
https://www.readbyqxmd.com/read/29776356/policy-development-and-challenges-of-global-mental-health-a-systematic-review-of-published-studies-of-national-level-mental-health-policies
#13
Wei Zhou, Yu Yu, Mei Yang, Lizhang Chen, Shuiyuan Xiao
BACKGROUND: Mental health policy can be an essential and powerful tool to improve a population's mental health. However, around one third of countries do not possess a mental health policy, and there are large disparities in population coverage rates between high- and low-income countries. The goal of this study is to identify the transition and implementation challenges of mental health policies in both high-income countries (HICs) as well as middle- and low-income countries (MLICs)...
May 18, 2018: BMC Psychiatry
https://www.readbyqxmd.com/read/29771639/hic-5-expression-is-a-major-indicator-of-cancer-cell-morphology-migration-and-plasticity-in-three-dimensional-matrices
#14
Anushree C Gulvady, Fatemeh Dubois, Nicholas O Deakin, Gregory J Goreczny, Christopher E Turner
The focal adhesion proteins, Hic-5 and Paxillin have been previously identified as key regulators of MDA-MB-231 breast cancer cell migration and morphologic mesenchymal-amoeboid plasticity in three-dimensional extracellular matrices. However, their respective roles in other cancer cell types has not been evaluated. Herein, utilizing 3D cell-derived matrices and fibronectin-coated 1D substrates, we show that across a variety of cancer cell lines, the level of Hic-5 expression serves as the major indicator of the cells primary morphology, plasticity and in vitro invasiveness...
May 17, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29769263/how-i-treat-burkitt-lymphoma-in-children-adolescents-and-young-adults-in-sub-saharan-africa
#15
Satish Gopal, Thomas G Gross
Burkitt lymphoma (BL) is the most common pediatric cancer in sub-Saharan Africa (SSA), and also occurs frequently among adolescents and young adults (AYA), often associated with human immunodeficiency virus (HIV). Treating BL in SSA poses particular challenges. Although highly effective, high-intensity cytotoxic treatments used in resource-rich settings are usually not feasible, and lower-intensity continuous infusion approaches are impractical. In this paper, based on evidence from the region, we review management strategies for SSA focused on diagnosis, use of pre-phase, and definitive treatment...
May 16, 2018: Blood
https://www.readbyqxmd.com/read/29765777/patterns-of-authorship-on-community-health-workers-in-low-and-middle-income-countries-an-analysis-of-publications-2012-2016
#16
Helen Schneider, Nelisiwe Maleka
Introduction: Studies of authorship provide a barometer of local research capacity and ownership of research, considered key to defining appropriate research priorities, developing contextualised responses to health problems and ensuring that research informs policy and practice. This paper reports on an analysis of patterns of research authorship of the now substantial literature on community health workers (CHWs) in low-and-middle-income countries (LMICs) for the 5-year period: 2012-2016...
2018: BMJ Global Health
https://www.readbyqxmd.com/read/29738565/different-chromatin-and-dna-sequence-characteristics-define-glucocorticoid-receptor-binding-sites-that-are-blocked-or-not-blocked-by-coregulator-hic-5
#17
Brian H Lee, Michael R Stallcup
The glucocorticoid receptor (GR) regulates genes in many physiological pathways by binding to enhancer and silencer elements of target genes, where it recruits coregulator proteins that remodel chromatin and regulate the assembly of transcription complexes. The coregulator Hydrogen peroxide-inducible clone 5 (Hic-5) is necessary for glucocorticoid (GC) regulation of one group of GR target genes, is irrelevant for a second group, and inhibits GR binding to a third gene set, thereby blocking their regulation by GC...
2018: PloS One
https://www.readbyqxmd.com/read/29715307/recognition-and-management-of-acute-kidney-injury-in-children-the-isn-0by25-global-snapshot-study
#18
Etienne Macedo, Jorge Cerdá, Sangeeta Hingorani, Jiayi Hou, Arvind Bagga, Emmanuel Almeida Burdmann, Michael Rocco V, Ravindra Mehta L
BACKGROUND: In low and middle-income countries, reliable data on the epidemiology of childhood acute kidney injury (AKI) is lacking. The Global Snapshot, conducted by the ISN "0by25" AKI initiative, was a world-wide cross-sectional, observational study to evaluate AKI in hospitalized patients. Here we report the pediatric results of this study. PATIENTS AND METHODS: We prospectively collected data on children who met the Kidney Disease Improving Global Outcomes AKI criteria during a 10-week window in late 2014...
2018: PloS One
https://www.readbyqxmd.com/read/29715303/health-insurance-coverage-with-or-without-a-nurse-led-task-shifting-strategy-for-hypertension-control-a-pragmatic-cluster-randomized-trial-in-ghana
#19
Gbenga Ogedegbe, Jacob Plange-Rhule, Joyce Gyamfi, William Chaplin, Michael Ntim, Kingsley Apusiga, Juliet Iwelunmor, Kwasi Yeboah Awudzi, Kofi Nana Quakyi, Jazmin Mogaverro, Kiran Khurshid, Bamidele Tayo, Richard Cooper
BACKGROUND: Poor access to care and physician shortage are major barriers to hypertension control in sub-Saharan Africa. Implementation of evidence-based systems-level strategies targeted at these barriers are lacking. We conducted a study to evaluate the comparative effectiveness of provision of health insurance coverage (HIC) alone versus a nurse-led task shifting strategy for hypertension control (TASSH) plus HIC on systolic blood pressure (SBP) reduction among patients with uncontrolled hypertension in Ghana...
May 2018: PLoS Medicine
https://www.readbyqxmd.com/read/29701556/estimating-the-global-incidence-of-traumatic-brain-injury
#20
Michael C Dewan, Abbas Rattani, Saksham Gupta, Ronnie E Baticulon, Ya-Ching Hung, Maria Punchak, Amit Agrawal, Amos O Adeleye, Mark G Shrime, Andrés M Rubiano, Jeffrey V Rosenfeld, Kee B Park
OBJECTIVE Traumatic brain injury (TBI)-the "silent epidemic"-contributes to worldwide death and disability more than any other traumatic insult. Yet, TBI incidence and distribution across regions and socioeconomic divides remain unknown. In an effort to promote advocacy, understanding, and targeted intervention, the authors sought to quantify the case burden of TBI across World Health Organization (WHO) regions and World Bank (WB) income groups. METHODS Open-source epidemiological data on road traffic injuries (RTIs) were used to model the incidence of TBI using literature-derived ratios...
April 27, 2018: Journal of Neurosurgery
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