Adebowale O Bamidele, Shravan K Mishra, Guilherme Piovezani Ramos, Petra Hirsova, Emily E Klatt, Leena M Abdelrahman, Mary R Sagstetter, Heidi M Davidson, Patrick J Fehrenbach, Lucia Valenzuela-Pérez, Hyun Se Kim Lee, Song Zhang, Abner Aguirre Lopez, Ahmed T Kurdi, Maria S Westphal, Michelle M Gonzalez, Joseph M Gaballa, Robyn Laura Kosinsky, Hee Eun Lee, Thomas C Smyrk, Glenn Bantug, Naomi M Gades, William A Faubion
BACKGROUND & AIMS: Incapacitated regulatory T cells (Tregs) contribute to immune-mediated diseases. Inflammatory Tregs are evident during human inflammatory bowel disease; however, mechanisms driving the development of these cells and their function are not well understood. Therefore, we investigated the role of cellular metabolism in Tregs relevant to gut homeostasis. METHODS: Using human Tregs, we performed mitochondrial ultrastructural studies via electron microscopy and confocal imaging, biochemical and protein analyses using proximity ligation assay, immunoblotting, mass cytometry and fluorescence-activated cell sorting, metabolomics, gene expression analysis, and real-time metabolic profiling utilizing the Seahorse XF analyzer...
January 23, 2024: Gastroenterology