keyword
MENU ▼
Read by QxMD icon Read
search

Proximity ligation assay

keyword
https://www.readbyqxmd.com/read/29777742/a-functional-glycoproteomics-approach-identifies-cd13-as-a-novel-e-selectin-ligand-in-breast-cancer
#1
M A Carrascal, M Silva, J A Ferreira, R Azevedo, D Ferreira, A M N Silva, D Ligeiro, L L Santos, R Sackstein, P A Videira
BACKGROUND: The glycan moieties sialyl-Lewis-X and/or -A (sLeX/A ) are the primary ligands for E-selectin, regulating subsequent tumor cell extravasation into distant organs. However, the nature of the glycoprotein scaffolds displaying these glycans in breast cancer remains unclear and constitutes the focus of the present investigation. METHODS: We isolated glycoproteins that bind E-selectin from the CF1_T breast cancer cell line, derived from a patient with ductal carcinoma...
May 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29773649/ptap-motif-duplication-in-the-p6-gag-protein-confers-a-replication-advantage-on-hiv-1-subtype-c
#2
Shilpee Sharma, Prabhu S Arunachalam, Malini Menon, Viswanath Ragupathy, Ravi Vijaya Satya, Joshua Jebaraj, Shambhu Ganeshappa Aralaguppe, Chaitra Rao, Sreshtha Pal, Shanmugam Saravanan, Kailapuri G Murugavel, Pachamuthu Balakrishnan, Suniti Solomon, Indira Hewlett, Udaykumar Ranga
HIV-1 subtype C (HIV-1C) may duplicate longer amino acid stretches in the p6 Gag protein, leading to the creation of an additional Pro-Thr/Ser-Ala-Pro (PTAP) motif necessary for viral packaging. However, the biological significance of a duplication of the PTAP motif for HIV-1 replication and pathogenesis has not been experimentally validated. In a longitudinal study of two different clinical cohorts of select HIV-1 seropositive, drug-naive individuals from India, we found that 8 of 50 of these individuals harbored a mixed infection of viral strains discordant for the PTAP duplication...
May 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29765307/a-novel-integrative-mechanism-in-anxiolytic-behavior-induced-by-galanin-2-neuropeptide-y-y1-receptor-interactions-on-medial-paracapsular-intercalated-amygdala-in-rats
#3
Manuel Narváez, Dasiel O Borroto-Escuela, Luis Santín, Carmelo Millón, Belén Gago, Antonio Flores-Burgess, Miguel A Barbancho, Miguel Pérez de la Mora, José Narváez, Zaida Díaz-Cabiale, Kjell Fuxe
Anxiety is evoked by a threatening situation and display adaptive or defensive behaviors, found similarly in animals and humans. Neuropeptide Y (NPY) Y1 receptor (NPYY1R) and Galanin (GAL) receptor 2 (GALR2) interact in several regions of the limbic system, including the amygdala. In a previous study, GALR2 enhanced NPYY1R mediated anxiolytic actions on spatiotemporal parameters in the open field and elevated plus maze, involving the formation of GALR2/NPYY1R heteroreceptor complexes in the amygdala. Moreover, the inclusion of complementary ethological parameters provides a more comprehensive profile on the anxiolytic effects of a treatment...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29743600/trafficking-localization-and-degradation-of-the-na-hco-3-co-transporter-nbcn1-in-kidney-and-breast-epithelial-cells
#4
Christina Wilkens Olesen, Jens Vogensen, Ida Axholm, Marc Severin, Julie Schnipper, Isabella Skandorff Pedersen, Jakob Hjorth von Stemann, Jacob Morville Schrøder, Dan Ploug Christensen, Stine Falsig Pedersen
The Na+;HCO3- co-transporter NBCn1 (SLC4A7) is a major regulator of intracellular pH yet its trafficking and turnover are essentially unstudied. Here, we used MDCK-II and MCF-7 cells to investigate these processes in epithelial cells. GFP-NBCn1 membrane localization was abolished by truncation of the full NBCn1 C-terminal tail (C-tail) yet did not require the C-terminal PDZ-binding motif (ETSL). Glutathione-S-Transferase-pulldown of the C-tail followed by mass spectrometry analysis revealed putative interactions with multiple sorting-, degradation- and retention factors, including the scaffolding protein RACK1...
May 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29743239/human-dhea-sulfation-requires-direct-interaction-between-paps-synthase-2-and-dhea-sulfotransferase-sult2a1
#5
Jonathan W Mueller, Jan Idkowiak, Tarsis F Gesteira, Cecilia Vallet, Rebecca Hardman, Johannes van den Boom, Vivek Dhir, Shirley K Knauer, Edina Rosta, Wiebke Arlt
The high-energy sulfate donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS), generated by human PAPS synthase isoforms PAPSS1 and PAPSS2, is required for all human sulfation pathways. Sulfotransferase SULT2A1 uses PAPS for sulfation of the androgen precursor dehydroepiandrosterone (DHEA), thereby reducing downstream activation of DHEA to active androgens. Human PAPSS2 mutations manifest with undetectable DHEA sulfate, androgen excess and metabolic disease, suggesting that ubiquitous PAPSS1 cannot compensate for deficient PAPSS2 in supporting DHEA sulfation...
May 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29736717/a-pla-irocs-pipeline-for-the-localization-of-protein-protein-interactions-in-situ
#6
Taras Pasternak, William Teale, Thorsten Falk, Benedetto Ruperti, Klaus Palme
In plants as well as other organisms, protein localization alone is insufficient to provide a mechanistic link between stimulus and process regulation. This is because protein-protein interactions are central to the regulation of biological processes. However, they remain very difficult to detect in situ, with the choice of tools for the detection of protein-protein interaction in situ still in need of expansion. Here, we provide a protocol for the detection and accurate localization of protein interactions based on the combination of a whole-mount proximity ligation assay and iRoCS, a coordinate system able to standardize subtle differences between the architecture of individual Arabidopsis roots...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29731755/expression-and-function-of-il12-23-related-cytokine-subunits-p35-p40-and-p19-in-giant-cell-arteritis-lesions-contribution-of-p40-to-th1-and-th17-mediated-inflammatory-pathways
#7
Georgina Espígol-Frigolé, Ester Planas-Rigol, Ester Lozano, Marc Corbera-Bellalta, Nekane Terrades-García, Sergio Prieto-González, Ana García-Martínez, Jose Hernández-Rodríguez, Josep M Grau, Maria C Cid
Background: Giant-cell arteritis (GCA) is considered a T helper (Th)1- and Th17-mediated disease. Interleukin (IL)-12 is a heterodimeric cytokine (p35/p40) involved in Th1 differentiation. When combining with p19 subunit, p40 compose IL-23, a powerful pro-inflammatory cytokine that maintains Th17 response. Objectives: The aims of this study were to investigate p40, p35, and p19 subunit expression in GCA lesions and their combinations to conform different cytokines, to assess the effect of glucocorticoid treatment on subunit expression, and to explore functional roles of p40 by culturing temporal artery sections with a neutralizing anti-human IL-12/IL-23p40 antibody...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29721083/targeting-cpt1a-mediated-fatty-acid-oxidation-sensitizes-nasopharyngeal-carcinoma-to-radiation-therapy
#8
Zheqiong Tan, Lanbo Xiao, Min Tang, Fang Bai, Jiangjiang Li, Liling Li, Feng Shi, Namei Li, Yueshuo Li, Qianqian Du, Jingchen Lu, Xinxian Weng, Wei Yi, Hanwen Zhang, Jia Fan, Jian Zhou, Qiang Gao, José N Onuchic, Ann M Bode, Xiangjian Luo, Ya Cao
Nasopharyngeal carcinoma (NPC) has a particularly high prevalence in southern China, southeastern Asia and northern Africa. Radiation resistance remains a serious obstacle to successful treatment in NPC. This study aimed to explore the metabolic feature of radiation-resistant NPC cells and identify new molecular-targeted agents to improve the therapeutic effects of radiotherapy in NPC. Methods : Radiation-responsive and radiation-resistant NPC cells were used as the model system in vitro and in vivo . Metabolomics approach was used to illustrate the global metabolic changes...
2018: Theranostics
https://www.readbyqxmd.com/read/29719262/augmentation-of-myc-dependent-mitotic-gene-expression-by-the-pygopus2-chromatin-effector
#9
Phillip G P Andrews, Catherine Popadiuk, Thomas J Belbin, Kenneth R Kao
Mitotic segregation of chromosomes requires precise coordination of many factors, yet evidence is lacking as to how genes encoding these elements are transcriptionally controlled. Here, we found that the Pygopus (Pygo)2 chromatin effector is indispensable for expression of the MYC-dependent genes that regulate cancer cell division. Depletion of Pygo2 arrested SKOV-3 cells at metaphase, which resulted from the failure of chromosomes to capture spindle microtubules, a critical step for chromosomal biorientation and segregation...
May 1, 2018: Cell Reports
https://www.readbyqxmd.com/read/29686000/angiotensin-ii-promotes-k-v-7-4-channels-degradation-through-reduced-interaction-with-hsp90-heat-shock-protein-90
#10
Vincenzo Barrese, Jennifer B Stott, Hericka B Figueiredo, Aisah A Aubdool, Adrian J Hobbs, Thomas A Jepps, Alister J McNeish, Iain A Greenwood
Voltage-gated Kv 7.4 channels have been implicated in vascular smooth muscle cells' activity because they modulate basal arterial contractility, mediate responses to endogenous vasorelaxants, and are downregulated in several arterial beds in different models of hypertension. Angiotensin II (Ang II) is a key player in hypertension that affects the expression of several classes of ion channels. In this study, we evaluated the effects of Ang II on the expression and function of vascular Kv 7.4. Western blot and quantitative polymerase chain reaction revealed that in whole rat mesenteric artery, Ang II incubation for 1 to 7 hours decreased Kv 7...
April 23, 2018: Hypertension
https://www.readbyqxmd.com/read/29676021/detection-of-alpha-synuclein-conformational-variants-from-gastro-intestinal-biopsy-tissue-as-a-potential-biomarker-for-parkinson-s-disease
#11
Claudio Ruffmann, Nora Bengoa-Vergniory, Ilaria Poggiolini, Diane Ritchie, Michele T Hu, Javier Alegre-Abarrategui, Laura Parkkinen
AIMS: Gastrointestinal (GI) α-synuclein (aSyn) detection as a potential biomarker of Parkinson's disease (PD) is challenged by conflicting results of recent studies. To increase sensitivity and specificity, we applied three techniques to detect different conformations of aSyn in GI biopsies obtained from a longitudinal, clinically well-characterized cohort of PD patients and healthy controls (HC). METHODS: With immunohistochemistry (IHC), we used antibodies reactive for total, phosphorylated and oligomeric aSyn; with aSyn proximity ligation assay (AS-PLA), we targeted oligomeric aSyn species specifically; and with paraffin-embedded tissue blot (AS-PET-blot) we aimed to detect fibrillary, synaptic aSyn...
April 19, 2018: Neuropathology and Applied Neurobiology
https://www.readbyqxmd.com/read/29673351/fk506-binding-protein-10-fkbp10-regulates-lung-fibroblast-migration-via-collagen-vi-synthesis
#12
Larissa Knüppel, Katharina Heinzelmann, Michael Lindner, Rudolf Hatz, Jürgen Behr, Oliver Eickelberg, Claudia A Staab-Weijnitz
BACKGROUND: In idiopathic pulmonary fibrosis (IPF), fibroblasts gain a more migratory phenotype and excessively secrete extracellular matrix (ECM), ultimately leading to alveolar scarring and progressive dyspnea. Here, we analyzed the effects of deficiency of FK506-binding protein 10 (FKBP10), a potential IPF drug target, on primary human lung fibroblast (phLF) adhesion and migration. METHODS: Using siRNA, FKBP10 expression was inhibited in phLF in absence or presence of 2ng/ml transforming growth factor-β1 (TGF-β1) and 0...
April 19, 2018: Respiratory Research
https://www.readbyqxmd.com/read/29651904/adaptor-protein-1-b-mu-subunit-does-not-contribute-to-the-recycling-of-kae1-protein-in-polarized-renal-epithelial-cells
#13
Ensaf Y Almomani, Nicolas Touret, Emmanuelle Cordat
Mutations in the gene encoding the kidney anion exchanger 1 (kAE1) can lead to distal renal tubular acidosis (dRTA). dRTA mutations reported within the carboxyl (C)-terminal tail of kAE1 result in apical mis-targeting of the exchanger in polarized renal epithelial cells. As kAE1 physically interacts with the μ subunit of epithelial adaptor protein 1 B (AP-1B), we investigated the role of heterologously expressed μ1B subunit of the AP-1B complex for kAE1 retention to the basolateral membrane in polarized porcine LLC-PK1 renal epithelial cells that are devoid of endogenous AP-1B...
April 13, 2018: Molecular Membrane Biology
https://www.readbyqxmd.com/read/29651006/cyclin-dependent-kinase-5-mediates-pleiotrophin-induced-endothelial-cell-migration
#14
Evgenia Lampropoulou, Ioanna Logoviti, Marina Koutsioumpa, Maria Hatziapostolou, Christos Polytarchou, Spyros S Skandalis, Ulf Hellman, Manolis Fousteris, Sotirios Nikolaropoulos, Efrosini Choleva, Margarita Lamprou, Angeliki Skoura, Vasileios Megalooikonomou, Evangelia Papadimitriou
Pleiotrophin (PTN) stimulates endothelial cell migration through binding to receptor protein tyrosine phosphatase beta/zeta (RPTPβ/ζ) and αν β3 integrin. Screening for proteins that interact with RPTPβ/ζ and potentially regulate PTN signaling, through mass spectrometry analysis, identified cyclin-dependent kinase 5 (CDK5) activator p35 among the proteins displaying high sequence coverage. Interaction of p35 with the serine/threonine kinase CDK5 leads to CDK5 activation, known to be implicated in cell migration...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649418/roles-of-progesterone-receptor-membrane-component-1-and-membrane-progestin-receptor-alpha-in-regulation-of-zebrafish-oocyte-maturation
#15
Joseph Aizen, Yefei Pang, Caleb Harris, Aubrey Converse, Yong Zhu, Meagan A Aguirre, Peter Thomas
Although previous studies suggest membrane progesterone receptor alpha (mPRα/Paqr7) mediates 17, 20β-dihydroxy-4-pregnen-3-one (DHP) induction of oocyte maturation (OM) in zebrafish, critical information needed to establish mPRα as the receptor mediating OM, is lacking. The relative potencies of progestins and specific mPRα agonists in inducing OM matched their relative binding affinities for zebrafish mPRα, supporting its role in OM. Microinjection of pertussis toxin blocked DHP induction of OM and the progestin-induced decrease in cyclic AMP levels, suggesting mPRα activates an inhibitory G protein (Gi)...
April 9, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29628759/the-development-of-azd7624-for-prevention-of-exacerbations-in-copd-a-randomized-controlled-trial
#16
Naimish R Patel, Danen M Cunoosamy, Malin Fagerås, Ziad Taib, Sara Asimus, Tove Hegelund-Myrbäck, Sofia Lundin, Katerina Pardali, Nisha Kurian, Eva Ersdal, Cecilia Kristensson, Katarina Korsback, Robert Palmér, Mary N Brown, Steven Greenaway, Leonard Siew, Graham W Clarke, Stephen I Rennard, Barry J Make, Robert A Wise, Paul Jansson
Background: p38 mitogen-activated protein kinase (MAPK) plays a central role in the regulation and activation of pro-inflammatory mediators. COPD patients have increased levels of activated p38 MAPK, which correlate with increased lung function impairment, alveolar wall inflammation, and COPD exacerbations. Objectives: These studies aimed to assess the effect of p38 inhibition with AZD7624 in healthy volunteers and patients with COPD. The principal hypothesis was that decreasing lung inflammation via inhibition of p38α would reduce exacerbations and improve quality of life for COPD patients at high risk for acute exacerbations...
2018: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/29623065/-in-situ-proximity-ligation-assay-reveals-co-localization-of-alpha-synuclein-and-snare-proteins-in-murine-primary-neurons
#17
Leire Almandoz-Gil, Emma Persson, Veronica Lindström, Martin Ingelsson, Anna Erlandsson, Joakim Bergström
The aggregation of alpha-synuclein (αSyn) is the pathological hallmark of Parkinson's disease, dementia with Lewy bodies and related neurological disorders. However, the physiological function of the protein and how this function relates to its pathological effects remain poorly understood. One of the proposed roles of αSyn is to promote the soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex assembly by binding to VAMP-2. The objective of this study was to visualize the co-localization between αSyn and the SNARE proteins (VAMP-2, SNAP-25, and syntaxin-1) for the first time using in situ proximity ligation assay (PLA)...
2018: Frontiers in Neurology
https://www.readbyqxmd.com/read/29622677/heparan-sulfate-antagonism-alters-bone-morphogenetic-protein-signaling-and-receptor-dynamics-suggesting-a-mechanism-in-hereditary-multiple-exostoses
#18
Christina Mundy, Evan Yang, Hajime Takano, Paul C Billings, Maurizio Pacifici
Hereditary Multiple Exostoses (HME) is a pediatric disorder caused by heparan sulfate (HS) deficiency and is characterized by growth plate-associated osteochondromas. Previously, we found that osteochondroma formation in mouse models is preceded by ectopic bone morphogenetic protein (BMP) signaling in the perichondrium, but the mechanistic relationships between BMP signaling and HS deficiency remain unclear. Therefore, we used an HS antagonist (Surfen) to investigate the effects of this HS interference on BMP signaling, ligand availability, cell surface BMP receptor (BMPR) dynamics and BMPR interactions in Ad-293 and C3H/10T1/2 cells...
April 5, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29614718/interaction-of-the-mouse-polyomavirus-capsid-proteins-with-importins-is-required-for-efficient-import-of-viral-dna-into-the-cell-nucleus
#19
Irina Soldatova, Terezie Prilepskaja, Levon Abrahamyan, Jitka Forstová, Sandra Huérfano
The mechanism used by mouse polyomavirus (MPyV) overcomes the crowded cytosol to reach the nucleus has not been fully elucidated. Here, we investigated the involvement of importin α/β1 mediated transport in the delivery of MPyV genomes into the nucleus. Interactions of the virus with importin β1 were studied by co-immunoprecipitation and proximity ligation assay. For infectivity and nucleus delivery assays, the virus and its capsid proteins mutated in the nuclear localization signals (NLSs) were prepared and produced...
March 31, 2018: Viruses
https://www.readbyqxmd.com/read/29610457/cntnap2-stabilizes-interneuron-dendritic-arbors-through-cask
#20
Ruoqi Gao, Nicolas H Piguel, Alexandria E Melendez-Zaidi, Maria Dolores Martin-de-Saavedra, Sehyoun Yoon, Marc P Forrest, Kristoffer Myczek, Gefei Zhang, Theron A Russell, John G Csernansky, D James Surmeier, Peter Penzes
Contactin associated protein-like 2 (CNTNAP2) has emerged as a prominent susceptibility gene implicated in multiple complex neurodevelopmental disorders, including autism spectrum disorders (ASD), intellectual disability (ID), and schizophrenia (SCZ). The presence of seizure comorbidity in many of these cases, as well as inhibitory neuron dysfunction in Cntnap2 knockout (KO) mice, suggests CNTNAP2 may be crucial for proper inhibitory network function. However, underlying cellular mechanisms are unclear. Here we show that cultured Cntnap2 KO mouse neurons exhibit an inhibitory neuron-specific simplification of the dendritic tree...
April 2, 2018: Molecular Psychiatry
keyword
keyword
104742
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"