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https://www.readbyqxmd.com/read/29169171/mirnas-are-unlikely-to-be-involved-in-retinoid-receptor-gene-regulation-in-pancreatic-cancer-cells
#1
Shuai Yin, Tim Bleul, Yifan Zhu, Orkhan Isayev, Jens Werner, Alexandr V Bazhin
BACKGROUND/AIMS: Retinoid receptors and retinoic acid were reported to be down-regulated in pancreatic duct adenocarcinoma (PDAC) compared to normal pancreas. Yet the mechanism of the down-regulation of retinoid receptors is not well defined. The aim of this study was to find out whether selected dysregulated miRNAs in PDAC are responsible for the decreased level of retinoid receptors. METHODS: Bioinformatics, real-time PCR, western blot analysis as well as molecular manipulation with miRNA in cells of PDAC were carried out...
November 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29169048/protein-lipoylation-an-evolutionarily-conserved-metabolic-regulator-of-health-and-disease
#2
REVIEW
Elizabeth A Rowland, Caroline K Snowden, Ileana M Cristea
Lipoylation is a rare, but highly conserved lysine posttranslational modification. To date, it is known to occur on only four multimeric metabolic enzymes in mammals, yet these proteins are staples in the core metabolic landscape. The dysregulation of these mitochondrial proteins is linked to a range of human metabolic disorders. Perhaps most striking is that lipoylation itself, the proteins that add or remove the modification, as well as the proteins it decorates are all evolutionarily conserved from bacteria to humans, highlighting the importance of this essential cofactor...
November 20, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29169046/a-novel-ccm1-krit1-heterozygous-deletion-mutation-c-1919delt-in-a-chinese-family-with-familial-cerebral-cavernous-malformation
#3
Chenlong Yang, Bingquan Wu, Haohao Zhong, Yan Li, Xingzheng Zheng, Yulun Xu
BACKGROUND: Cerebral cavernous malformation (CCM) is a relatively rare congenital vascular anomaly in the central venous system. Its inherited form, familial cerebral cavernous malformation (FCCM), is an autosomal-dominant disease with incomplete penetrance. The pathogenic genes of FCCM have been mapped into three loci: CCM1/KRIT1, CCM2/MGC4607, and CCM3/PDCD10. Till now, the genetic basis of FCCM in the Chinese population has yet to be well understood. Herein, we investigated the genetic mutation in a Chinese family with FCCM...
November 20, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29169041/the-shewanella-woodyi-galactokinase-pool-phosphorylates-glucose-at-the-6-position
#4
Louis Patrick Conway, Fang Fang Liu, Qian Li, Josef Voglmeir
Galactokinases are a class of enzymes which belong to the GHMP (galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase) superfamily and catalyse the phosphorylation of galactose in the first step of the Leloir pathway. Here we report the discovery of three enzymes from Shewanella woodyi which have been classified as galactokinases based on sequence similarity. However, each of these enzymes show little to no significant activity towards galactose and instead exhibit a strong preference for glucose...
October 31, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/29168754/a-bioinformatic-pipeline-for-monitoring-of-the-mutational-stability-of-viral-drug-targets-with-deep-sequencing-technology
#5
Yuri Kravatsky, Vladimir Chechetkin, Daria Fedoseeva, Maria Gorbacheva, Galina Kravatskaya, Olga Kretova, Nickolai Tchurikov
The efficient development of antiviral drugs, including efficient antiviral small interfering RNAs (siRNAs), requires continuous monitoring of the strict correspondence between a drug and the related highly variable viral DNA/RNA target(s). Deep sequencing is able to provide an assessment of both the general target conservation and the frequency of particular mutations in the different target sites. The aim of this study was to develop a reliable bioinformatic pipeline for the analysis of millions of short, deep sequencing reads corresponding to selected highly variable viral sequences that are drug target(s)...
November 23, 2017: Viruses
https://www.readbyqxmd.com/read/29168730/comparative-proteomics-analyses-of-pollination-response-in-endangered-orchid-species-dendrobium-chrysanthum
#6
Wei Wang, Hongyang Yu, Tinghai Li, Lexing Li, Guoqiang Zhang, Zhongjian Liu, Tengbo Huang, Yongxia Zhang
Pollination is a crucial stage in plant reproductive process. The self-compatibility (SC) and self-incompatibility (SI) mechanisms determined the plant genetic diversity and species survival. D. chrysanthum is a highly valued ornamental and traditional herbal orchid in Asia but has been declared endangered. The sexual reproduction in D. chrysanthum relies on the compatibility of pollination. To provide a better understanding of the mechanism of pollination, the differentially expressed proteins (DEP) between the self-pollination (SP) and cross-pollination (CP) pistil of D...
November 23, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29168723/agro-ecological-compensation-of-watershed-based-on-emergy
#7
Yicheng Fu, Xia Du, Benqing Ruan, Laisheng Liu, Jian Zhang
The paper aims at providing a framework to calculate the agro-eco-compensation standard based on the systems agro-ecological concept of embodied energy as emergy. We have proposed a calculation system of eco-compensation standard for sustainable agricultural development based on the convertibility between emergy and price. According to our calculation, the total energy of agricultural production in Yongding River Basin was 3.45 E + 16 Sej/ha (the unit emergy value, expressed in solar emergy joules per unit) in terms of the renewable resources, nonrenewable resources, material inputs, and service costs...
November 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29168674/breast-conserving-surgery-versus-mastectomy-overall-and-relative-survival-a-population-based-study-by-the-danish-breast-cancer-cooperative-group-dbcg
#8
Peer Christiansen, Stina Lyck Carstensen, Bent Ejlertsen, Niels Kroman, Birgitte Offersen, Anne Bodilsen, Maj-Britt Jensen
BACKGROUND: Observational studies have pointed at a better survival after breast conserving surgery (BCS) compared with mastectomy. The aim of the present study was to evaluate whether this remains true when more extensive tumor characteristics and treatment data were included. METHODS: The cohort included patients registered after primary surgery for early invasive breast cancer in the database of the Danish Breast Cancer Cooperative Group, in the period 1995-2012...
November 23, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/29168638/homology-modeling-and-molecular-dynamics-simulation-combined-with-x-ray-solution-scattering-defining-protein-structures-of-thromboxane-and-prostacyclin-synthases
#9
Hsiao-Ching Yang, Cheng-Han Yang, Ming Yi Huang, Jyh-Feng Lu, Jinn-Shyan Wang, Yi-Qi Yeh, U-Ser Jeng
A combination of molecular dynamics (MD) simulations and X-ray scattering (SAXS) has emerged as the approach of choice for studying protein structures and dynamics in solution. This approach has potential applications for membrane proteins that neither are soluble nor form crystals easily. We explore the water-coupled dynamic structures of thromboxane synthase (TXAS) and prostacyclin synthase (PGIS) from scanning HPLC-SAXS measurements combined with MD ensemble analyses. Both proteins are heme-containing enzymes in the cytochrome P450 family, known as prostaglandin H2 (PGH2) isomerase, with counter functions in regulation of platelet aggregation...
November 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29168508/structural-basis-for-the-initiation-of-eukaryotic-transcription-coupled-dna-repair
#10
Jun Xu, Indrajit Lahiri, Wei Wang, Adam Wier, Michael A Cianfrocco, Jenny Chong, Alissa A Hare, Peter B Dervan, Frank DiMaio, Andres E Leschziner, Dong Wang
Eukaryotic transcription-coupled repair (TCR) is an important and well-conserved sub-pathway of nucleotide excision repair that preferentially removes DNA lesions from the template strand that block translocation of RNA polymerase II (Pol II). Cockayne syndrome group B (CSB, also known as ERCC6) protein in humans (or its yeast orthologues, Rad26 in Saccharomyces cerevisiae and Rhp26 in Schizosaccharomyces pombe) is among the first proteins to be recruited to the lesion-arrested Pol II during the initiation of eukaryotic TCR...
November 22, 2017: Nature
https://www.readbyqxmd.com/read/29168500/a-lysosomal-switch-triggers-proteostasis-renewal-in-the-immortal-c-elegans-germ-lineage
#11
K Adam Bohnert, Cynthia Kenyon
Although individuals age and die with time, an animal species can continue indefinitely, because of its immortal germ-cell lineage. How the germline avoids transmitting damage from one generation to the next remains a fundamental question in biology. Here we identify a lysosomal switch that enhances germline proteostasis before fertilization. We find that Caenorhabditis elegans oocytes whose maturation is arrested by the absence of sperm exhibit hallmarks of proteostasis collapse, including protein aggregation...
November 22, 2017: Nature
https://www.readbyqxmd.com/read/29168495/a-unifying-paradigm-for-naphthoquinone-based-meroterpenoid-bio-synthesis
#12
Zachary D Miles, Stefan Diethelm, Henry P Pepper, David M Huang, Jonathan H George, Bradley S Moore
Bacterial meroterpenoids constitute an important class of natural products with diverse biological properties and therapeutic potential. The biosynthetic logic for their production is unknown and defies explanation via classical biochemical paradigms. A large subgroup of naphthoquinone-based meroterpenoids exhibits a substitution pattern of the polyketide-derived aromatic core that seemingly contradicts the established reactivity pattern of polyketide phenol nucleophiles and terpene diphosphate electrophiles...
December 2017: Nature Chemistry
https://www.readbyqxmd.com/read/29168427/neoadjuvant-letrozole-for-postmenopausal-estrogen-receptor-positive-her2-negative-breast-cancer-patients-a-study-from-the-danish-breast-cancer-cooperative-group-dbcg
#13
Signe Korsgaard Skriver, Anne-Vibeke Laenkholm, Birgitte Bruun Rasmussen, Jürgen Handler, Bo Grundtmann, Tove Filtenborg Tvedskov, Peer Christiansen, Ann S Knoop, Maj-Britt Jensen, Bent Ejlertsen
INTRODUCTION: Neoadjuvant endocrine treatment (NET) is a low-toxicity approach to achieve operability in locally advanced breast cancer, and to facilitate breast conservation in early breast cancer, particular in patients with highly estrogen receptor (ER) positive and HER2-negative disease. Here, we report the results obtained by neoadjuvant letrozole in patients with early breast cancer in a phase-II design. MATERIAL AND METHODS: A total of 119 postmenopausal women with ER-positive, HER2-negative operable breast cancer were assigned to four months of neoadjuvant letrozole before definitive surgery...
November 23, 2017: Acta Oncologica
https://www.readbyqxmd.com/read/29168417/a-dna-segment-encoding-the-anticodon-stem-loop-of-trna-determines-the-specific-recombination-of-integrative-conjugative-elements-in-acidithiobacillus-species
#14
Andrés Castillo, Mario Tello, Kenneth Ringwald, Lillian G Acuña, Raquel Quatrini, Omar Orellana
Horizontal gene transfer is crucial for the adaptation of microorganisms to environmental cues. The acidophilic, bioleaching bacterium Acidithiobacillus ferrooxidans encodes an integrative-conjugative genetic element (ICEAfe1) inserted in the gene encoding a tRNA(Ala). This genetic element is actively excised from the chromosome upon induction of DNA damage. A similar genetic element (ICEAcaTY.2) is also found in an equivalent position in the genome of Acidithiobacillus caldus. The local genomic context of both mobile genetic elements is highly syntenous and the cognate integrases are well conserved...
November 23, 2017: RNA Biology
https://www.readbyqxmd.com/read/29168350/breakpoint-mapping-and-haplotype-analysis-of-translocation-t-1-12-q43-q21-1-in-two-apparently-independent-families-with-vascular-phenotypes
#15
Tiia Maria Luukkonen, Mana M Mehrjouy, Minna Pöyhönen, Anna-Kaisa Anttonen, Päivi Lahermo, Pekka Ellonen, Lars Paulin, Niels Tommerup, Aarno Palotie, Teppo Varilo
BACKGROUND: The risk of serious congenital anomaly for de novo balanced translocations is estimated to be at least 6%. We identified two apparently independent families with a balanced t(1;12)(q43;q21.1) as an outcome of a "Systematic Survey of Balanced Chromosomal Rearrangements in Finns." In the first family, carriers (n = 6) manifest with learning problems in childhood, and later with unexplained neurological symptoms (chronic headache, balance problems, tremor, fatigue) and cerebral infarctions in their 50s...
November 23, 2017: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29168288/robotic-strong-s-procedure-for-the-treatment-of-superior-mesenteric-artery-syndrome-description-of-surgical-technique-on-occasion-of-the-first-reported-case-in-the-literature
#16
Harilaos Konstantinidis, Christos Charisis, Panagiotis Kottos
BACKROUND: Superior Mesenteric Artery Syndrome (SMAS) is a rare disorder caused by compression of the third portion of the duodenum by the SMA. Once a conservative approach fails, usual surgical strategies include Duodenojejunostomy and Strong's procedure. The latter avoids potential anastomotic risks and complications. Robotic Strong's procedure (RSP) combines both the benefits of a minimal invasive approach and also enchased robotic accuracy and efficacy. METHODS: For a young girl who was unsuccessfully treated conservatively, the paper describes the RSP surgical technique...
November 23, 2017: International Journal of Medical Robotics + Computer Assisted Surgery: MRCAS
https://www.readbyqxmd.com/read/29168227/alternatives-to-biodiversity-offsets-for-mitigating-the-effects-of-urbanization-on-stream-ecosystems
#17
Myles E Coker, Nick R Bond, Yung En Chee, Christopher J Walsh
Globally, offset schemes have emerged in many statutory frameworks relating to development activities, with the aim of balancing biodiversity conservation and development. While the theory and use of biodiversity offsets in terrestrial environments is broadly documented, little attention has been paid to offsets in stream ecosystems. Here we examine the application of offset schemes to stream ecosystems and explore whether they suffer similar shortcomings to those of offset schemes focused on terrestrial biodiversity...
November 23, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/29168224/dynamics-in-the-global-protected-area-estate-since-2004
#18
Edward Lewis, Brian MacSharry, Diego Juffe-Bignoli, Nyeema Harris, Georgina Burrows, Naomi Kingston, Neil D Burgess
Nations of the world have committed to a number of goals and targets to address the global environmental challenges humanity faces. Protected areas have for centuries been a key strategy in conservation and play a major role in addressing current challenges. The most important tool used to track progress on protected area commitments is the World Database on Protected Areas (WDPA). Periodic assessments of the world's protected area estate show steady growth over the last two decades. However, the current method, which uses the latest version of the WDPA, does not show the true dynamic nature of protected areas over time, nor does it provide information on sites removed from the WDPA...
November 23, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/29167987/the-geographical-vector-in-distribution-of-genetic-diversity-for-clonorchis-sinensis
#19
Daria A Solodovnik, Yulia V Tatonova, Polina V Burkovskaya
Clonorchis sinensis, the causative agent of clonorchiasis, is one of the most important parasites that inhabit countries of East and Southeast Asia. In this study, we validated the existence of a geographical vector for C. sinensis using the partial cox1 mtDNA gene, which includes a conserved region. The samples of parasite were divided into groups corresponding to three river basins, and the size of the conserved region had a strong tendency to increase from the northernmost to the southernmost samples. This indicates the availability of the geographical vector in distribution of genetic diversity...
November 22, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29167954/no-implant-migration-and-good-subjective-outcome-of-a-novel-customized-femoral-resurfacing-metal-implant-for-focal-chondral-lesions
#20
Anders Stålman, Olof Sköldenberg, Nicolas Martinez-Carranza, David Roberts, Magnus Högström, Leif Ryd
PURPOSE: Managing focal cartilage injuries in the middle-aged patient poses a challenge. Focal prosthetic inlay resurfacing has been proposed to be a bridge between biologics and conventional joint arthroplasty. Patient selection and accurate implant positioning is crucial to avoid increased contact pressure to the opposite cartilage surface. A customized femoral condyle implant for focal cartilage injuries was designed to precisely fit each patient's individual size and location of damage...
November 22, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
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