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Soluble egfr serum cancer

Line Brøndum, Brita Singers Sørensen, Jesper Grau Eriksen, Lise Saksø Mortensen, Simon Lønbro, Jens Overgaard, Jan Alsner
OBJECTIVE: To assess the usability of archived plasma and serum by multiplex (Luminex) analysis of circulating proteins (analytes) by evaluating the day to day variation, the effect of several freeze-thaw cycles, and the influence of the media and choice of anticoagulant. METHODS: Nineteen analytes in plasma and serum from 86 head and neck cancer patients and 33 controls were evaluated: EGFR, leptin, OPN, VEGFR-1, VEGFR-2, IL-2, IL-13, PDGF-bb, TNF, PAI-1, SDF-1a, IL-4, IL-6, IL-8, eotaxin, G-CSF, VEGF, GRO-a, and HGF...
September 26, 2016: Scandinavian Journal of Clinical and Laboratory Investigation
Steffen Filskov Sorensen, Christina Demuth, Britta Weber, Boe Sandahl Sorensen, Peter Meldgaard
OBJECTIVES: The central immune co-inhibitory programmed cell death receptor/ligand 1 (PD-1/PD-L1) pathway plays a key role in tumor immune evasion in non-small cell lung cancer (NSCLC). Soluble PD-1 (sPD-1) can be detected in the blood, and preclinical evidence suggests that sPD-1 blocks PD-1/-L1 interaction and improves anti-tumor immunity. The present study compares the concentration of sPD-1 in the serum of advanced NSCLC patients with Epidermal Growth Factor Receptor (EGFR) mutation prior to erlotinib treatment and at the time of progression and correlates these results to patient outcome...
October 2016: Lung Cancer: Journal of the International Association for the Study of Lung Cancer
Praveenkumar Shetty, Vidya Shankar Patil, Rajshekar Mohan, Leonard Clinton D'souza, Anil Bapu Bargale, Basavaraj R Patil, Dinesh U S, Vikram Haridas, Shrirang P Kulkarni
BACKGROUND: AnnexinA2 (AnxA2) membrane deposition has a critical role in HB-EGF shedding as well as IL-6 secretion in breast cancer cells. This autocrine cycle has a major role in cancer cell proliferation, migration and metastasis. The objective of the study is to demonstrate AnxA2 mediated autocrine regulation via HB-EGF and IL-6 in Her-2 negative breast cancer progression. METHODS: Secretory AnxA2, HB-EGF and IL-6 were analysed in the peripheral blood sample of Her-2 negative (n=20) and positive breast cancer patients (n=16)...
August 5, 2016: Annals of Clinical Biochemistry
Adrian G Sacher, Lisa W Le, Humberto Lara-Guerra, Thomas K Waddell, Shingo Sakashita, Zhuo Chen, Lucia Kim, Tong Zhang, Suzanne Kamel-Reid, Alexandra Salvarrey, Gail Darling, Kazuhiro Yasufuku, Shaf Keshavjee, Marc de Perrot, Frances A Shepherd, Geoffrey Liu, Ming Sound Tsao, Natasha B Leighl
BACKGROUND: Erlotinib is highly active in EGFR mutant NSCLC, but may benefit some with wild-type tumors. We examined pre-operative erlotinib in early stage NSCLC to assess response and correlation with potential biomarkers. RESULTS: Twenty-five patients were enrolled; 22 received erlotinib treatment and were evaluable (median follow-up 4.4 years). Histology was predominantly adenocarcinoma although 31% had squamous carcinoma. PET response was observed in 2 patients (9%), both with squamous carcinoma...
May 3, 2016: Oncotarget
Xu Wan, Xiaoyao Zheng, Xiaoying Pang, Zheming Zhang, Qizhi Zhang
Lapatinib, a selective small-molecule dual-tyrosine kinase inhibitor of HER2 and EGFR, is effective in HER2-positive patients with advanced metastatic breast cancer. However, its low and variable oral absorption, large required daily dose and serious gastrointestinal side effects all limit its clinical use. Intravenous administration offers a good option to overcome these disadvantages. However, the poor solubility of lapatinib in water and organic solvents causes lapatinib to fail in a common injectable preparation...
December 1, 2015: Colloids and Surfaces. B, Biointerfaces
Pete Heinzelman, John Krais, Eliza Ruben, Robert Pantazes
BACKGROUND: Engineered antibodies with pH responsive cell surface target antigen-binding affinities that decrease at the acidic pH (5.5-5.8) within the endosomes have been found to have reduced susceptibility to degradation within the lysosomes and increased serum half-life. Such pH responsive recombinant antibodies have been developed for the treatment of cancer and cardiovascular disease. Engineered tenth type III human fibronectin (Fn3) domains are emerging as a class of target antigen-binding biopharmaceuticals that could complement or be superior to recombinant antibodies in a number of biomedical contexts...
2015: Journal of Biological Engineering
Heng Hu, Lingling Gao, Cun Wang, Yan Li, Huiying Ma, Long Chen, Jie Qin, Binbin Liu, Yinkun Liu, Chunmin Liang
Hepatocellular carcinoma (HCC) is one of the most deadly cancers in the world due to its high metastatic potential. By using the isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative N-glycoproteomic analysis, 26 differentially expressed serum glycoproteins derived from defined stages in orthotopic xenograft tumor model were identified. Among them, expression level of soluble EGFR (sEGFR) was verified in HCC cell lines. We found that non-metastasis HCC cell lines express significantly more sEGFR than HCC cell lines with metastasis potential both in cell lysates and culture media...
May 2015: Discovery Medicine
Elena Yaiza Romero-Ventosa, Sonia Blanco-Prieto, Ana Lourdes González-Piñeiro, Francisco Javier Rodríguez-Berrocal, Guadalupe Piñeiro-Corrales, María Páez de la Cadena
The aim of this study has been to investigate the potential of serum biomarkers used in clinical practice (CEA, CYFRA 21-1, SCC) together with the serum epidermal growth factor receptor (EGFR) and its associated ligands (EGF, TGF-α, HB-EGF) as outcome predictors of non-small cell lung cancer (NSCLC) patients treated with the TKI erlotinib. The pretreatment levels of these markers were evaluated through immunoassays carried out in 58 patients. The progression-free survival (PFS) and overall survival (OS) were assessed by the Kaplan-Meier method and differences between groups were compared by means of the Log-Rank test...
2015: SpringerPlus
Xu Wan, Xiaoyao Zheng, Xiaoying Pang, Zheming Zhang, Tao Jing, Wei Xu, Qizhi Zhang
Triple-negative breast cancer (TNBC) is an aggressive cancer with limited treatment options. However, the shared feature of epidermal growth factor receptor (EGFR) expression in TNBC offers the opportunity for targeted molecular therapy for this breast cancer subtype. Previous studies have indicated that lapatinib, a selective small-molecular dual-tyrosine kinase inhibitor of HER2 and EGFR, is effective in reducing cancer progression and metastasis, indicating that it might be a candidate for TNBC treatment...
April 30, 2015: International Journal of Pharmaceutics
Young Pil Kim, Dongsun Park, Jae Jin Kim, Won-Jae Chi, Won-Jae Choi, Sun Hee Lee, Seo Yun Lee, Soyeon Kim, Jee Min Chung, Jinseon Jeon, Byoung Dae Lee, Joo-Ho Shin, Yun-Il Lee, Hyeseong Cho, Jeong-Min Lee, Ho Chul Kang
Cetuximab, a chimeric monoclonal antibody developed for targeting the Epidermal Growth Factor Receptor (EGFR), has been intensively used to treat cancer patients with metastatic colorectal cancer and head and neck cancer. Intact immunoglobulin G (IgG) antibody like cetuximab, however, has some limitations such as high production cost and low penetration rate from vasculature into solid tumor mass due to its large size. In attempt to overcome these limitations, we engineered cetuximab to create single chain variable fragments (scFv-CH3; Minibody) that were expressed in bacterial system...
2014: PloS One
Jason A Wilken, Marianela Perez-Torres, Rene Nieves-Alicea, Elsa M Cora, Trace A Christensen, Andre T Baron, Nita J Maihle
Soluble epidermal growth factor receptor (sEGFR) is a circulating serum biomarker in cancer patients. Recent studies suggest that baseline serum sEGFR concentrations may predict responsiveness to EGFR-targeted therapy. Here, we demonstrate that sEGFR is generated through proteolytic cleavage of a cell surface precursor of an alternately spliced EGF receptor isoform and that sEGFR binds to EGF with high affinity. Proteolytic cleavage is stimulated by an anti-α5/β1 integrin antibody and 4-aminophenylmercuric acetate, and inhibited by fibronectin...
July 2, 2013: Biochemistry
Carol Box, Marta Mendiola, Sharon Gowan, Gary M Box, Melanie Valenti, Alexis De Haven Brandon, Bissan Al-Lazikani, Susanne J Rogers, Anna Wilkins, Kevin J Harrington, Suzanne A Eccles
BACKGROUND: Acquired resistance to tyrosine kinase inhibitors (TKIs) is becoming a major challenge in the treatment of many cancers. Epidermal growth factor receptor (EGFR) is overexpressed in squamous carcinomas, notably those of the head and neck (HNSCC), and can be targeted with several TKIs. We aimed to identify soluble proteins suitable for development as markers of EGFR TKI resistance in cancer patients to aid in early and minimally invasive assessment of therapeutic responses. METHODS: Resistant HNSCC cell lines were generated by exposure to an EGFR TKI, gefitinib, in vitro...
July 2013: European Journal of Cancer
Kimberly K Leslie, Michael W Sill, Edgar Fischer, Kathleen M Darcy, Robert S Mannel, Krishnansu S Tewari, Parviz Hanjani, Jason A Wilken, Andre T Baron, Andrew K Godwin, Russell J Schilder, Meenakshi Singh, Nita J Maihle
BACKGROUND: A phase II trial was performed to evaluate the efficacy and safety of gefitinib in patients with persistent/recurrent endometrial cancer. METHODS: Women with histologically confirmed persistent/recurrent endometrial cancer were treated with 500mg oral gefitinib daily until progression or severe toxicity, with progression-free survival (PFS) at six months as the primary endpoint. Tumor expression of total epidermal growth factor receptor (EGFR), estrogen receptor (ER), progesterone receptor A (PRA) and B (PRB), Ki67, pEGFR and activated extracellular signal-regulated kinase (pERK) were examined pre- and post-treatment...
June 2013: Gynecologic Oncology
Minkyung Kang, Suyong Choi, Soo-Jin Jeong, Sin-Ae Lee, Tae Kyoung Kwak, Hyeonjung Kim, Oisun Jung, Mi-Sook Lee, Youra Ko, Jihye Ryu, Yoon-Ju Choi, Doyoung Jeong, Hyo Jeong Lee, Sang-Kyu Ye, Sung-Hoon Kim, Jung Weon Lee
The EMT (epithelial-mesenchymal transition) is involved in fibrosis and cancer, and is regulated by different signalling pathways mediated through soluble factors, actin reorganization and transcription factor actions. Because the tetraspan (also called tetraspanin) TM4SF5 (transmembrane 4 L6 family member 5) is highly expressed in hepatocellular carcinoma and induces EMT, understanding how TM4SF5 expression in hepatocytes is regulated is important. We explored the mechanisms that induce TM4SF5 expression and whether impaired signalling pathways for TM4SF5 expression inhibit the acquisition of mesenchymal cell features, using human and mouse normal hepatocytes...
May 1, 2012: Biochemical Journal
I Kappers, M A Vollebergh, H van Tinteren, C M Korse, L L Nieuwenhuis, J M G Bonfrer, H M Klomp, N van Zandwijk, M M van den Heuvel
BACKGROUND: In patients with non-small cell lung cancer (NSCLC), a higher response rate can be achieved with epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) when selection for therapy is guided by mutation analysis or gene amplification. However, both tests are complex and require tumour tissue. Simple methods to identify responders prior to EGFR-TKI treatment are urgently needed. This study aimed to define the relation between serum sEGFR levels, carcinoembryonic antigen (CEA) and survival in NSCLC patients treated with EGFR-TKIs...
2010: Ecancermedicalscience
Kamila A Adamczyk, Susanne Klein-Scory, Mahnaz Moradian Tehrani, Uwe Warnken, Wolff Schmiegel, Martina Schnölzer, Irmgard Schwarte-Waldhoff
AIMS: Members of the epidermal growth factor receptor (EGFR) family represent validated targets for anti-cancer therapy and EGFR inhibitors have also shown efficacy in pancreatic carcinoma. We here described in detail molecular forms of the EGF receptor released by pancreatic cancer cells. We found peptides specific for the EGFR in the secretomes of five pancreatic cancer cell lines. Secretomes from cultured cancer cells are widely used as sources for serum biomarker discovery. MAIN METHODS: The detailed analysis of EGFR forms in secretomes of human pancreatic cancer cells is a compilation of results from mass spectrometry (MS) and Western blotting with intracellular and extracellular domain specific antibodies...
August 29, 2011: Life Sciences
Landon J Inge, Sonali P Barwe, Julia D'Ambrosio, Jegan Gopal, Kan Lu, Sergey Ryazantsev, Sigrid A Rajasekaran, Ayyappan K Rajasekaran
High levels of the soluble form of E-cadherin can be found in the serum of cancer patients and are associated with poor prognosis. Despite the possible predictive value of soluble E-cadherin, little is understood concerning its patho-physiological consequences in tumor progression. In this study, we show that soluble E-cadherin facilitates cell survival via functional interaction with cellular E-cadherin. Exposure of cells to a recombinant form of soluble E-cadherin, at a concentration found in cancer patient's serum, prevents apoptosis due to serum/growth factor withdrawal, and inhibits epithelial lumen formation, a process that requires apoptosis...
April 1, 2011: Experimental Cell Research
Tess V Clendenen, Alan A Arslan, Anna E Lokshin, Annika Idahl, Göran Hallmans, Karen L Koenig, Adele M Marrangoni, Brian M Nolen, Nina Ohlson, Anne Zeleniuch-Jacquotte, Eva Lundin
BACKGROUND: Cytokines are involved in the development of chronic diseases, including cancer. It is important to evaluate the temporal reproducibility of cytokines in plasma prior to conducting epidemiologic studies utilizing these markers. FINDINGS: We assessed the temporal reliability of CRP, 22 cytokines and their soluble receptors (IL-1α, IL-1β, IL-1RA, IL-2, sIL-2R, IL-4, IL-5, IL-6, sIL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17, TNFα, sTNF-R1, sTNF-R2, IFNα, IFNγ) and eight growth factors (GM-CSF, EGF, bFGF, G-CSF, HGF, VEGF, EGFR, ErbB2) in repeated EDTA plasma samples collected an average of two years apart from 18 healthy women (age range: 42-62) enrolled in a prospective cohort study...
2010: BMC Research Notes
Andrea L Russo, Deborah Citrin, Kevin Camphausen
The discovery of biomarkers in patients receiving radiation therapy for cancer is occurring at an exceptional pace. There are a number of ways to conduct biomarker investigations, although the majority of clinically relevant biomarker studies have used immunohistochemistry (IHC) on tissue specimens. Using IHC, expression of several pre-radiation biomarkers, such as VEGF, EGFR, YKL-40, murine double minute 2 and Rad51, has been associated with a poor outcome. Because tumor tissue may be difficult to obtain and IHC studies can be difficult to reproduce and standardize, investigators have attempted to find similar results when studying biomarkers of nontumor biospecimens, such as serum and urine...
April 2008: Biomarkers in Medicine
Andre T Baron, Jason A Wilken, Daniel E Haggstrom, Scott T Goodrich, Nita J Maihle
Signal transduction pathways regulated by the EGFR/ERBB/HER proto-oncogene family and receptor tyrosine kinases encoded by these genes are known to become dysregulated during cellular transformation and carcinogenesis. Consequently, biologically targeted antibodies and tyrosine kinase inhibitors directed toward EGFR/ErbB1/HER1 (eg, cetuximab, erlotinib and gefitinib) and ErbB2/HER2 (eg, trastuzumab), and more recently toward ErbB3/HER3 and ErbB4/HER4, are being investigated as therapeutic agents for treating patients with EGFR/ERBB/HER proto-oncogene-driven malignancies...
May 2009: IDrugs: the Investigational Drugs Journal
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