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https://www.readbyqxmd.com/read/28333932/impairment-of-wnt-%C3%AE-catenin-signaling-in-blood-cells-of-patients-with-severe-cavitary-pulmonary-tuberculosis
#1
Lin Fan, Hongbo Shen, Huichang Huang, Rui Yang, Lan Yao
Tuberculosis (TB) remains as a leading infectious disease worldwide. Our previous study showed interferon (IFN)-γ and CD3 T cell impairment in patients with severe cavitary pulmonary TB (PTB). However, the cause of the change in immune responses during the progression of TB is still poorly understood. In this study, eight newly diagnosed patients with severe cavitary and mild lesion non-cavity PTB were recruited, and three healthy volunteers were recruited as the control. RNA extracted from blood was tested by whole genome oligo microarrays...
2017: PloS One
https://www.readbyqxmd.com/read/28331616/ex-vivo-akt-inhibition-promotes-the-generation-of-potent-cd19car-t-cells-for-adoptive-immunotherapy
#2
Ryan Urak, Miriam Walter, Laura Lim, ChingLam W Wong, Lihua E Budde, Sandra Thomas, Stephen J Forman, Xiuli Wang
BACKGROUND: Insufficient persistence and effector function of chimeric antigen receptor (CAR)-redirected T cells have been challenging issues for adoptive T cell therapy. Generating potent CAR T cells is of increasing importance in the field. Studies have demonstrated the importance of the Akt pathway in the regulation of T cell differentiation and memory formation. We now investigate whether inhibition of Akt signaling during ex vivo expansion of CAR T cells can promote the generation of CAR T cells with enhanced antitumor activity following adoptive therapy in a murine leukemia xenograft model...
2017: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/28327177/intestinal-t-lymphocyte-homing-is-associated-with-gastric-emptying-and-epithelial-barrier-function-in-critically-ill-a-prospective-observational-study
#3
Christian Greis, Zohal Rasuly, Rolf A Janosi, Lambros Kordelas, Dietrich W Beelen, Tobias Liebregts
BACKGROUND: Impaired gastric emptying is common in critically ill patients. Intestinal dysmotility, a major cause of feed intolerance, may foster infectious complications due to mucosal barrier disruption. However, little is known about gut-directed immune activation, intestinal barrier function and its association with impaired gastric emptying in critically ill patients at ICU admission. METHODS: We conducted a prospective observational study at two tertiary care medical ICUs...
March 22, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/28326171/large-scale-isolation-and-cytotoxicity-of-extracellular-vesicles-derived-from-activated-human-natural-killer-cells
#4
Ambrose Y Jong, Chun-Hua Wu, Jingbo Li, Jianping Sun, Muller Fabbri, Alan S Wayne, Robert C Seeger
Extracellular vesicles (EVs) have been the focus of great interest, as they appear to be involved in numerous important cellular processes. They deliver bioactive macromolecules such as proteins, lipids, and nucleic acids, allowing intercellular communication in multicellular organisms. EVs are secreted by all cell types, including immune cells such as natural killer cells (NK), and they may play important roles in the immune system. Currently, a large-scale procedure to obtain functional NK EVs is lacking, limiting their use clinically...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28298232/elutriated-lymphocytes-for-manufacturing-chimeric-antigen-receptor-t-cells
#5
David F Stroncek, Daniel W Lee, Jiaqiang Ren, Marianna Sabatino, Steven Highfill, Hanh Khuu, Nirali N Shah, Rosandra N Kaplan, Terry J Fry, Crystal L Mackall
BACKGROUND: Clinical trials of Chimeric Antigen Receptor (CAR) T cells manufactured from autologous peripheral blood mononuclear cell (PBMC) concentrates for the treatment of hematologic malignancies have been promising, but CAR T cell yields have been variable. This variability is due in part to the contamination of the PBMC concentrates with monocytes and granulocytes. METHODS: Counter-flow elutriation allows for the closed system separation of lymphocytes from monocytes and granulocytes...
March 16, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28273783/multiple-sclerosis-serum-derived-exosomes-express-myelin-proteins
#6
Grazyna Galazka, Marcin P Mycko, Igor Selmaj, Cedric S Raine, Krzysztof W Selmaj
BACKGROUND: Exosomes are small extracellular vesicles that provide cell-to-cell communication and are involved in immunoregulation. OBJECTIVE: To investigate serum exosomes for the presence of myelin proteins outside the central nervous system (CNS) and their role in multiple sclerosis (MS). METHODS: Serum, cerebrospinal fluid (CSF), and peripheral blood mononuclear cell (PBMC) samples were collected from 45 patients with relapsing-remitting MS (RRMS), 30 patients with secondary progressive MS (SPMS), and 45 healthy controls...
February 1, 2017: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/28265771/expression-dynamics-of-hsp90-and-nitric-oxide-synthase-nos-isoforms-during-heat-stress-acclimation-in-tharparkar-cattle
#7
Jaya Bharati, S S Dangi, S Bag, V P Maurya, G Singh, P Kumar, M Sarkar
Six male Tharparkar cattle of 2-3 years old were selected for the study. After 15-day acclimation at thermoneutral zone (TNZ) in psychrometric chamber, animals were exposed at 42 °C for 6 h up to 23 days followed by 12 days of recovery period. Blood samples were collected during control period at TNZ (days 1, 5, and 12), after heat stress exposure (day 1, immediate heat stress acclimation (IHSA); days 2 to 10, short-term heat stress acclimation (STHSA); days 15 to 23, long-term heat stress acclimation (LTHSA); days 7 and 12, recovery period), and peripheral blood mononuclear cells (PBMCs) were isolated for RNA and protein extraction...
March 7, 2017: International Journal of Biometeorology
https://www.readbyqxmd.com/read/28253713/secalonic-acid-f-inhibited-cell-growth-more-effectively-than-5-fluorouracil-on-hepatocellular-carcinoma-in-vitro-and-in-vivo
#8
X Gao, H L Sun, D S Liu, J R Zhang, J Zhang, M M Yan, X H Pan
Hepatocellular carcinoma (HCC), one of the most common types of liver cancer, could be treated with 5-fluorouracil (5-FU). Due to its side effects, 5-FU is more often used as the co-administration drug in clinical practice. Secalonic acid-F (SAF), isolated from a fungal strain identified in our lab as Aspergillus aculeatus, showed potent biological activities. The goal of this study was to evaluate the inhibitory effects of SAF on hepatocellular carcinoma and to compare it with that of 5-FU. Results showed that SAF effectively inhibited cell growth with a dose-dependent manner in vitro and in vivo...
March 3, 2017: Neoplasma
https://www.readbyqxmd.com/read/28250797/changes-in-peripheral-immune-cell-numbers-and-functions-in-octogenarian-walkers-an-acute-exercise-study
#9
Kornelis S M van der Geest, Qi Wang, Thijs M H Eijsvogels, Hans J P Koenen, Irma Joosten, Elisabeth Brouwer, Maria T E Hopman, Joannes F M Jacobs, Annemieke M H Boots
BACKGROUND: Age-related changes of the immune system, termed immunosenescence, may underlie the increased risk of infections and morbidity in the elderly. Little is known about the effects of acute exercise on peripheral immune parameters in octogenarians. Therefore, we investigated acute exercise-induced changes in phenotype and function of the immune system in octogenarians participating in the 2013 edition of the Nijmegen Four Days Marches. Blood sampling was performed at baseline and immediately after 4 days of the walking exercise (30 km/day)...
2017: Immunity & Ageing: I & A
https://www.readbyqxmd.com/read/28246044/dna-methylation-and-expression-of-stress-related-genes-in-pbmc-of-mdd-patients-with-and-without-serious-suicidal-ideation
#10
Bhaskar Roy, Richard C Shelton, Yogesh Dwivedi
Stress plays an important role in major depressive disorder (MDD) and is one of the state dependent factors in suicidal behavior. A dysfunctional hypothalamic-pituitary-adrenal axis is a common feature in this disorder. The involvement of environmental factors has added additional complexity to understanding depression or suicidal behavior. In this regard, epigenetic regulation has been considered a mechanistic interface between environmental stress stimuli and altered functioning of underlying gene network that may increase susceptibility to depression or suicidal behavior...
February 16, 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28245380/-decitabine-enhances-the-sensitivity-of-leukemia-stem-cell-to-allo-nk-cell-mediated-killing
#11
Qian Li, Shan-Shan Wei, Jin-Gao Li, Shao-Xian Chen, Jing Chen, Hui-Ting Huang, Qi Peng, Ping-Fang Xia, Miao-Rong She
OBJECTIVE: To investigate the allo-NK cell-mediated killing effect enhanced by decitabine on leukemia stem cells(LSC) and the underlying mechanisms. METHODS: LSC were separated from KG1a cells by using immunomagnetic beads. Allo-NK cells were isolated and purified from PBMC of healthy donors. Cytotoxicity of allo-NK cells against LSC were measured by LDH releasing assay. The apoptosis induced by allo-NK cells in LSC and the expressions of NKG2D ligands including MICA/B and ULBP1-3 on LSC were detected by flow cytometry...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28243867/peripheral-blood-lymphocyte-hiv-dna-levels-correlate-with-hiv-associated-neurocognitive-disorders-in-nigeria
#12
Jibreel Jumare, Sara Sunshine, Hayat Ahmed, Samer S El-Kamary, Laurence Magder, Laura Hungerford, Tricia Burdo, Lindsay M Eyzaguirre, Anya Umlauf, Mariana Cherner, Alash'le Abimiku, Man Charurat, Jonathan Z Li, William A Blattner, Walter Royal
Mononuclear cells play key roles in the pathogenic mechanisms leading to HIV-associated neurocognitive disorders (HANDs). We examined the association between HIV DNA within peripheral blood mononuclear cell (PBMC) subsets and HAND in Nigeria. PBMCs were collected at baseline from 36 antiretroviral naive participants. CD14+ cells and T&B lymphocyte fractions were isolated by, respectively, positive and negative magnetic bead separation. Total HIV DNA within CD14+ and T&B cells were separately quantified using real-time PCR assay targeting HIV LTR-gag and cell input numbers determined by CCR5 copies/sample...
February 27, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/28239378/enterococcus-durans-ep1-a-promising-anti-inflammatory-probiotic-able-to-stimulate-siga-and-to-increase-faecalibacterium-prausnitzii-abundance
#13
Paula Carasi, Silvia María Racedo, Claudine Jacquot, Anne Marie Elie, María de Los Ángeles Serradell, María C Urdaci
Enterococcus species, principally Enterococcus faecium are used as probiotics since a long time with preference in animal applications but safety considerations were updated and also new uses as probiotics can be envisaged. Fifteen Enterococcus strains isolated from different foods were identified and analyzed for virulence factors and antibiotic resistance. Three Enterococcus durans strains were selected to study their immunomodulatory properties on PBMC and Caco2 cells. Two strains presented a profile toward a mild inflammatory Th1 response considering TNF-α/IL-10 and IL-1β/IL-10 cytokines ratios...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28236541/immune-response-of-toddlers-with-history-of-prematurity
#14
S P Muraro, P M Pitrez, A P D de Souza, B N Porto, J E Vargas, I P Ewald, J P Heinzmann-Filho, G Dos Santos, T S Baptista, T D Gandolfi, F D Machado, M H Jones, C Bonorino, R T Stein
BACKGROUND: It is not quite well established how immune responses differ in term and preterm infants beyond the first year of life. This study aimed to evaluate aspects of the innate and adaptive immune responses in a group of preterm infants in comparison with their term peers. METHODS: In this cross-sectional study peripheral blood mononuclear cells (PBMC) were isolated from preterm and term children at age three years. Innate immune response was evaluated by the analysis of TLR receptors expression on CD11c(+)HLADR(high) cells and inflammatory cytokine production after PBMC stimulation with Toll like receptors (TLR) ligands...
February 21, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28236450/evaluation-of-4-thiazolidinone-derivatives-as-potential-reverse-transcriptase-inhibitors-against-hiv-1-drug-resistant-strains
#15
Rahul Suryawanshi, Sushama Jadhav, Nandini Makwana, Dipen Desai, Devidas Chaturbhuj, Archana Sonawani, Susan Idicula-Thomas, Vanangamudi Murugesan, Seturam B Katti, Srikanth Tripathy, Ramesh Paranjape, Smita Kulkarni
Rapid emergence of drug resistance is crucial in management of HIV infection limiting implementation of efficacious drugs in the ART regimen. Designing new molecules against HIV drug resistant strains is utmost essential. Based on the anti-HIV-1 activity, we selected four 4-thiazolidinone derivatives (S009-1908, S009-1909, S009-1911, S009-1912) and studied their interaction with reverse transcriptase (RT) from a panel of 10 clinical isolates (8 nevirapine resistant and two susceptible) using in silico methods, and inhibition pattern using in vitro cell based assays...
February 15, 2017: Bioorganic Chemistry
https://www.readbyqxmd.com/read/28226398/dysregulation-of-interleukin-5-expression-in-familial-eosinophilia
#16
Senbagavalli Prakash Babu, Yun-Yun K Chen, Sandra Bonne-Annee, Jun Yang, Irina Maric, Timothy G Myers, Thomas B Nutman, Amy D Klion
BACKGROUND: Familial eosinophilia (FE) is a rare autosomal dominant inherited disorder characterized by the presence of lifelong peripheral eosinophilia (>1500/μL). Mapped to chromosome 5q31-q33, the genetic cause of FE is unknown, and prior studies have failed to demonstrate a primary abnormality in the eosinophil lineage. OBJECTIVE: The aim of the present study was to identify the cells driving the eosinophilia in FE. METHODS: Microarray analysis and real-time PCR were used to examine transcriptional differences in peripheral blood mononuclear cells (PBMC), and in purified cell subsets from affected and unaffected family members belonging to a single large kindred...
February 22, 2017: Allergy
https://www.readbyqxmd.com/read/28209523/tb-iris-proteomic-analysis-of-in-vitro-pbmc-responses-to-mycobacterium-tuberculosis-and-response-modulation-by-dexamethasone
#17
Liam Bell, Janique M Peyper, Shaun Garnett, Rabecca Tadokera, Robert Wilkinson, Graeme Meintjes, Jonathan M Blackburn
Paradoxical tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) occurs in 8-54% of South African patients undergoing treatment for tuberculosis/human immunodeficiency virus co-infection. Improved TB-IRIS molecular pathogenesis understanding would enhance risk stratification, diagnosis, prognostication, and treatment. We assessed how TB-IRIS status and dexamethasone influence leukocyte proteomic responses to Mycobacterium tuberculosis (Mtb). Patient blood was obtained three weeks post-anti-retroviral therapy initiation...
February 14, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28208127/prevalence-of-occult-hepatitis-c-virus-infection-among-blood-donors-in-jiangsu-china
#18
Hong Lin, Xian Chen, Shaowen Zhu, Ping Mao, Shanshan Zhu, Yanchun Liu, Chengyin Huang, Jun Sun, Jin Zhu
OBJECTIVE: In 2010, only 1 donor blood sample was found to be anti-hepatitis C virus (HCV) negative and HCV RNA positive, as detected by nucleic acid testing. In occult HCV infection (OCI), HCV RNA is found in peripheral blood mononuclear cells (PBMCs). We investigated the prevalence of OCI among blood donors. METHODS: We collected 513 samples from 334 eligible and 179 deferred donors, including 55 anti-HCV-positive, 113 alanine aminotransferase (ALT)-elevated, and 11 hepatitis B virus surface antigen (HBsAg)-positive blood donors...
February 17, 2017: Intervirology
https://www.readbyqxmd.com/read/28202529/aberrant-syk-kinase-signaling-is-essential-for-tumorigenesis-induced-by-tsc2-inactivation
#19
Ye Cui, Wendy K Steagall, Anthony M Lamattina, Gustavo Pacheco-Rodriguez, Mario Stylianou, Pranav Kidambi, Benjamin Stump, Fernanda Golzarri, Ivan O Rosas, Carmen Priolo, Elizabeth P Henske, Joel Moss, Souheil El-Chemaly
Somatic or germline mutations in the tuberous sclerosis complex (TSC) tumor suppressor genes are associated closely with the pathogenesis of lymphangioleiomyomatosis, a rare and progressive neoplastic disease that predominantly affects women in their childbearing years. Serum levels of the lymphangiogenic growth factor VEGF-D are elevated significantly in lymphangioleiomyomatosis. However, there are gaps in knowledge regarding VEGF-D dysregulation and its cellular origin in lymphangioleiomyomatosis. Here, we show that increased expression and activation of the tyrosine kinase Syk in TSC2-deficient cells and pulmonary nodules from lymphangioleiomyomatosis patients contributes to tumor growth...
March 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28199728/altered-mir-155-expression-in-allergic-asthmatic-airways
#20
Carina Malmhäll, Kristina Johansson, Carla Winkler, Sahar Alawieh, Linda Ekerljung, Madeleine Rådinger
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at posttranscriptional level. We and others have previously identified miRNAs with pathological roles in animal models of asthma. To date, few studies have investigated miRNA expression in human asthmatics and miRNA expression in specific cellular subtypes in asthmatics is largely unexplored. Therefore, the aim of this study was to assess miR-146a and miR-155 expression in airways- and blood-derived samples, two miRNA candidates which have previously been described to play important roles in inflammatory processes...
February 15, 2017: Scandinavian Journal of Immunology
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