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https://www.readbyqxmd.com/read/28910385/improving-the-characterization-of-endothelial-progenitor-cell-subsets-by-an-optimized-facs-protocol
#1
Karin Huizer, Dana A M Mustafa, J Clarissa Spelt, Johan M Kros, Andrea Sacchetti
The characterization of circulating endothelial progenitor cells (EPCs) is fundamental to any study related to angiogenesis. Unfortunately, current literature lacks consistency in the definition of EPC subsets due to variations in isolation strategies and inconsistencies in the use of lineage markers. Here we address critical points in the identification of hematopoietic progenitor cells (HPCs), circulating endothelial cells (CECs), and culture-generated outgrowth endothelial cells (OECs) from blood samples of healthy adults (AB) and umbilical cord (UCB)...
2017: PloS One
https://www.readbyqxmd.com/read/28892515/strong-interferon-gamma-mediated-cellular-immunity-to-scrub-typhus-demonstrated-using-a-novel-whole-cell-antigen-elispot-assay-in-rhesus-macaques-and-humans
#2
Manutsanun Sumonwiriya, Daniel H Paris, Piyanate Sunyakumthorn, Tippawan Anantatat, Kemajittra Jenjaroen, Suchintana Chumseng, Rawiwan Im-Erbsin, Ampai Tanganuchitcharnchai, Suthatip Jintaworn, Stuart D Blacksell, Fazle R Chowdhury, Barbara Kronsteiner-Dobramysl, Prapit Teparrukkul, Robin L Burke, Eric D Lombardini, Allen L Richards, Carl J Mason, James W Jones, Nicholas P J Day, Susanna J Dunachie
Scrub typhus is a febrile infection caused by the obligate intracellular bacterium Orientia tsutsugamushi, which causes significant morbidity and mortality across the Asia-Pacific region. The control of this vector-borne disease is challenging due to humans being dead-end hosts, vertical maintenance of the pathogen in the vector itself, and a potentially large rodent reservoir of unclear significance, coupled with a lack of accurate diagnostic tests. Development of an effective vaccine is highly desirable. This however requires better characterization of the natural immune response of this neglected but important disease...
September 11, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28870461/enhanced-plasmacytoid-dendritic-cell-antiviral-responses-after-omalizumab
#3
Michelle A Gill, Andrew H Liu, Agustin Calatroni, Rebecca Z Krouse, Baomei Shao, Allison Schiltz, James E Gern, Alkis Togias, William W Busse
BACKGROUND: Atopy and viral respiratory infections synergistically promote asthma exacerbations. IgE cross-linking inhibits critical viral-induced IFNα responses of plasmacytoid dendritic cells (pDCs), which can be deficient in allergic asthma. OBJECTIVE: To determine whether reducing IgE in vivo with omalizumab treatment increases pDC antiviral IFNα responses in inner city children with asthma. METHODS: PBMCs and pDCs isolated from children with exacerbation-prone asthma before and during omalizumab treatment were stimulated ex vivo with rhinovirus and influenza in the presence or absence of IgE cross-linking...
September 1, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28870237/the-n-and-c-terminal-carbohydrate-recognition-domains-of-haemonchus-contortus-galectin-bind-to-distinct-receptors-of-goat-pbmc-and-contribute-differently-to-its-immunomodulatory-functions-in-host-parasite-interactions
#4
MingMin Lu, XiaoWei Tian, XinChao Yang, Cheng Yuan, Muhammad Ehsan, XinChao Liu, RuoFeng Yan, LiXin Xu, XiaoKai Song, XiangRui Li
BACKGROUND: Hco-gal-m is a tandem-repeat galectin isolated from the adult worm of Haemonchus contortus. A growing body of studies have demonstrated that Hco-gal-m could exert its immunomodulatory effects on host peripheral blood mononuclear cells (PBMC) to facilitate the immune evasion. Our previous work revealed that C-terminal and N-terminal carbohydrate recognition domains (CRD) of Hco-gal-m had different sugar binding abilities. However, whether different domains of Hco-gal-m account differently for its multiple immunomodulatory functions in the host-parasite interaction remains to be elucidated...
September 5, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28860929/cd300lg-improves-the-cytotoxic-activity-of-cik
#5
Qiuyue Wang, Yuqing Liu, Ying Chen, Ke Wang, Wensheng Xie, Dapeng Wei, Lijuan Hu
To investigate the effect of CD300LG-γ induction on the cytotoxic activity of CIK. Eukaryotic expression plasmid hCD300LG-γ/pEGFP-C3, which can express human CD300LG-γ, was constructed and transfected into CHO cells by lipofectamine. The expression of CD300LG-γ was confirmed by immunofluorescence, RT-PCR, and Western Blot. To produce CIK cells, human peripheral blood mononuclear cells (PBMC) were isolated and induced, respectively, by cell lysates extracted from hCD300LG-γ/CHO cells, pEGFP-C3/CHO cells, and CHO cells, concurrently with the standard CIK inductive agent...
2017: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/28836673/evaluation-of-gene-expression-levels-for-cytokines-in-ocular-toxoplasmosis
#6
Marta Marques Maia, Cristina Silva Meira-Strejevitch, Vera Lucia Pereira-Chioccola, Daise Damaris Carnietto de Hippólito, Valéria Oliveira Silva, Cinara Cássia Brandão de Mattos, Fábio Batista Frederico, Rubens Camargo Siqueira, Luiz Carlos de Mattos
This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) of ocular toxoplasmosis patients (OT Group, n= 23) and chronic toxoplasmosis individuals (CHR Group, n= 9) were isolated and stimulated in vitro with T. gondii antigen. Negative controls (NC) were constituted of 7 PBMC samples from individuals seronegative for toxoplasmosis. mRNA expression for cytokines was determined by qPCR. Results showed a significant increase of mRNA levels from antigen stimulated PBMCs derived from OT Group for expressing IL-6 (at p<0...
August 24, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28835578/altered-immune-responses-in-broiler-chicken-husbandry-workers-and-their-association-with-endotoxin-exposure
#7
Ravi Gautam, Yong Heo, GyeongDong Lim, EunSeob Song, Katharine Roque, JaeHee Lee, YeonGyeong Kim, AhRang Cho, SoJung Shin, ChangYul Kim, GiHwan Bang, JiYun Bahng, HyoungAh Kim
Exposure to bioaerosols in indoor animal farms associates with respiratory illnesses, but little is known about the immune modulation to chicken farmers. This study aimed to compare the general immunity of chicken farmers with those of control subjects with non-agricultural jobs. Blood taken from the farmers and controls was subjected to plasma IgE and IgG subclass measurements. Isolated peripheral blood mononuclear cells (PBMC) were stimulated and cytokine production was measured. Indoor total and respirable dust levels and their endotoxin (LPS) and aflatoxin (AF) levels in the farms were measured...
August 22, 2017: Industrial Health
https://www.readbyqxmd.com/read/28822915/immunomodulatory-effects-of-m2000-%C3%AE-d-mannuronic-acid-on-tnf-%C3%AE-il-17-and-foxp3-gene-expression-in-patients-with-inflammatory-bowel-disease
#8
Hussaini Alhassan Mohammed, Ali Akbar Saboor-Yaraghi, Homayoun Vahedi, Mir Saeed Yekaninejad, Ghodratollah Panahi, Gholamreza Hemmasi, Mostafa Lakzaei, Abbas Mirshafiey
INTRODUCTION: Inflammatory bowel diseases (IBD) are immune-mediated disorders that result from an aberrant immunological response to the gut luminal antigen in genetically susceptible patients. IBD is categorized into two serotype, Crohn's diseases (CD) and ulcerative colitis (UC), both subtype are important cause of gastrointestinal diseases. The increasing rate of hospitalization, with the high economic burden experienced by the IBD patients, calls for more concerted research efforts to design a potent and affordable treatment option for the treatment of IBD...
August 17, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28822407/-clinical-observation-on-the-effect-of-glucocorticoid-insensitivity-on-sudden-sensorineural-hearing-loss-patients
#9
J Hou, H Qi, Y H Dai, X M Wei, L Lu, J G Wang, W D She
Objective: To investigate the correlation between the proliferation inhibition effect of glucocorticoid (GC) on peripheral blood mononuclear cell (PBMC) and the pure tone average (PTA) improvement in SSNHL patients. Methods: Sixty inpatients with SSNHL were included from July 2013 to October 2015 in Nanjing Drum Tower Hospital, Medical School of Nanjing University. Peripheral venous blood was collected before receiving treatment, then the PBMC was isolated for GC proliferation inhibition. PBMCs of each patient were cultivated into 4 groups: Group A: PBMCs+ Medium; Group B: PBMCs+ Medium+ lipopolysaccharide (LPS, 1 μmol/L); Group C: PBMCs+ Medium+ LPS+ Dexamethasone; Group D: Medium...
August 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
https://www.readbyqxmd.com/read/28818060/rna-transfection-of-%C3%AE-%C3%AE-t-cells-with-a-chimeric-antigen-receptor-or-an-%C3%AE-%C3%AE-t-cell-receptor-a-safer-alternative-to-genetically-engineered-%C3%AE-%C3%AE-t-cells-for-the-immunotherapy-of-melanoma
#10
Dennis C Harrer, Bianca Simon, Shin-Ichiro Fujii, Kanako Shimizu, Ugur Uslu, Gerold Schuler, Kerstin F Gerer, Stefanie Hoyer, Jan Dörrie, Niels Schaft
BACKGROUND: Adoptive T-cell therapy relying on conventional T cells transduced with T-cell receptors (TCRs) or chimeric antigen receptors (CARs) has caused substantial tumor regression in several clinical trials. However, genetically engineered T cells have been associated with serious side-effects due to off-target toxicities and massive cytokine release. To obviate these concerns, we established a protocol adaptable to GMP to expand and transiently transfect γ/δ T cells with mRNA...
August 17, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28792466/distinctive-responses-in-an-in-vitro-human-dendritic-cell-based-system-upon-stimulation-with-different-influenza-vaccine-formulations
#11
Gabriela Tapia-Calle, Maaike Stoel, Jacqueline de Vries-Idema, Anke Huckriede
Vaccine development relies on testing vaccine candidates in animal models. However, results from animals cannot always be translated to humans. Alternative ways to screen vaccine candidates before clinical trials are therefore desirable. Dendritic cells (DCs) are the main orchestrators of the immune system and the link between innate and adaptive responses. Their activation by vaccines is an essential step in vaccine-induced immune responses. We have systematically evaluated the suitability of two different human DC-based systems, namely the DC-cell line MUTZ-3 and primary monocyte-derived DCs (Mo-DCs) to screen immunopotentiating properties of vaccine candidates...
August 9, 2017: Vaccines
https://www.readbyqxmd.com/read/28770666/the-influence-of-vitamin-d-on-m1-and-m2-macrophages-in-patients-with-crohn-s-disease
#12
Serge Dionne, Carl-Frederic Duchatelier, Ernest G Seidman
Defective bacterial clearance by macrophages plays an important role in Crohn's disease (CD). Phenotypes and functions of inflammatory M1 and anti-inflammatory M2 have not been studied in CD. Vitamin D supplementation reduces the severity of CD by unclear mechanisms. We studied macrophage characteristics in CD and controls and the effects of 1,25 vitamin D (1,25D). PBMC were isolated from CD patients and controls. M1 and M2 were generated by culturing of monocytes with GM-CSF and M-CSF, respectively. CD M1 and M2 showed normal phagocytosis and chemotaxis to CCL2 and fMLP...
August 2017: Innate Immunity
https://www.readbyqxmd.com/read/28756292/anticancer-potential-of-new-steroidal-thiazolidin-4-one-derivatives-mechanisms-of-cytotoxic-action-and-effects-on-angiogenesis-in-vitro
#13
Marijana B Živković, Ivana Z Matić, Marko V Rodić, Irena T Novaković, Ana M Krivokuća, Dušan M Sladić, Natalija M Krstić
The synthesis and cytotoxic activities determination of new steroidal mono- and bis(thiazolidin-4-ones) 4a-f and 5a-f have been performed. Their anticancer action was also evaluated in comparison to previously synthesized and reported corresponding steroidal thiosemicarbazones. All compounds were obtained as stereoisomeric mixtures with different configuration (E or Z) in the hydrazone moiety at the C-3 position. After several consecutive crystallizations diastereomerically pure major (E)-isomers of mono-thiazolidin-4-ones were isolated...
July 26, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28756234/anti-tumor-necrosis-factor-with-a-glyco-engineered-fc-region-has-increased-efficacy-in-mice-with-colitis
#14
Felicia M Bloemendaal, Alon D Levin, Manon E Wildenberg, Pim J Koelink, Bradford L McRae, Jochen Salfeld, Jenifer Lum, Marijn van der Neut Kolfschoten, Jill W Claassens, Remco Visser, Arthur Bentlage, Geert R A M D'Haens, J Sjef Verbeek, Gestur Vidarsson, Gijs R van den Brink
BACKGROUND & AIMS: Although tumor necrosis factor (TNF) antagonists reduce many clinical features of inflammatory bowel disease (IBD), complete mucosal healing occurs in less than 50% of patients. The Fc region of monoclonal antibodies against TNF has immunosuppressive properties via effects on macrophage polarization. We examined the interaction between the anti-TNF Fc region and Fcγ receptors (FcγR), and whether the absence of the Fc core fucose (which increases binding to FcγRIIIa) increases the efficacy of anti-TNF in mice with colitis...
July 26, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28744288/naltrexone-inhibits-il-6-and-tnf%C3%AE-production-in-human-immune-cell-subsets-following-stimulation-with-ligands-for-intracellular-toll-like-receptors
#15
Rachel Cant, Angus G Dalgleish, Rachel L Allen
The opioid antagonist naltrexone hydrochloride has been suggested to be a potential therapy at low dosage for multiple inflammatory conditions and cancers. Little is known about the immune-modulating effects of naltrexone, but an effect on the activity of toll-like receptor 4 (TLR4) has been reported. We analyzed the effects of naltrexone hydrochloride on IL-6 secretion by peripheral blood mononuclear cells (PBMC) in vitro following stimulation with ligands for TLR4 and for the intracellular receptors TLR7, TLR8, and TLR9...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28734742/efficacy-of-a-nonadjuvanted-recombinant-felv-vaccine-and-two-inactivated-felv-vaccines-when-subject-to-consistent-virulent-felv-challenge-conditions
#16
Deborah A Grosenbaugh, Valérie Frances-Duvert, Shabnam Abedi, Bradley Feilmeier, Hongyu Ru, Hervé Poulet
The purpose of this study was to compare the efficacy of three FeLV vaccines, under identical conditions in a laboratory challenge model that closely mimics natural infection. Four groups of cats (n = 20 per group) were administered two doses of vaccine, 21 days apart, starting at 9-10 weeks of age (Purevax(®) FeLV, Versifel(®) FeLV, Nobivac(®) feline 2-FeLV, and a placebo). Cats were challenged 3 weeks later with a virulent, heterologous FeLV isolate. FeLV antigenemia was determined at weekly intervals from 3 to 15 weeks postchallenge...
September 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28719653/isolation-of-a-monoclonal-antibody-from-a-phage-display-library-binding-the-rhesus-macaque-mhc-class-i-allomorph-mamu-a1-001
#17
Nathan Holman, Jason T Weinfurter, Trevor R Harsla, Roger W Wiseman, Aaron J Belli, Anthony J Michaels, Keith A Reimann, Robert I DeMars, Matthew R Reynolds
Monoclonal antibodies that bind to human leukocyte antigen (HLA) are useful tools for HLA-typing, tracking donor-recipient chimerisms after bone marrow transplants, and characterizing specific major histocompatibility complexes (MHC) on cell surfaces. Unfortunately, equivalent reagents are not available for rhesus macaques, which are commonly used animal as models in organ transplant and infectious disease research. To address this deficiency, we isolated an antibody that recognizes the common Indian rhesus macaque MHC class I molecule, Mamu-A1*001...
2017: PloS One
https://www.readbyqxmd.com/read/28714870/antiviral-lipopeptide-cell-membrane-interaction-is-influenced-by-peg-linker-length
#18
Marcelo T Augusto, Axel Hollmann, Matteo Porotto, Anne Moscona, Nuno C Santos
A set of lipopeptides was recently reported for their broad-spectrum antiviral activity against viruses belonging to the Paramyxoviridae family, including human parainfluenza virus type 3 and Nipah virus. Among them, the peptide with a 24-unit PEG linker connecting it to a cholesterol moiety (VG-PEG24-Chol) was found to be the best membrane fusion inhibitory peptide. Here, we evaluated the interaction of the same set of peptides with biomembrane model systems and isolated human peripheral blood mononuclear cells (PBMC)...
July 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28713353/functional-characterization-of-novel-faecalibacterium-prausnitzii-strains-isolated-from-healthy-volunteers-a-step-forward-in-the-use-of-f-prausnitzii-as-a-next-generation-probiotic
#19
Rebeca Martín, Sylvie Miquel, Leandro Benevides, Chantal Bridonneau, Véronique Robert, Sylvie Hudault, Florian Chain, Olivier Berteau, Vasco Azevedo, Jean M Chatel, Harry Sokol, Luis G Bermúdez-Humarán, Muriel Thomas, Philippe Langella
Faecalibacterium prausnitzii is a major member of the Firmicutes phylum and one of the most abundant bacteria in the healthy human microbiota. F. prausnitzii depletion has been reported in several intestinal disorders, and more consistently in Crohn's disease (CD) patients. Despite its importance in human health, only few microbiological studies have been performed to isolate novel F. prausnitzii strains in order to better understand the biodiversity and physiological diversity of this beneficial commensal species...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28707376/immunosenescence-induced-by-plasma-from-individuals-with-obesity-caused-cell-signaling-dysfunction-and-inflammation
#20
Mariana Migliorini Parisi, Lucas Kich Grun, Patrícia Lavandoski, Letícia Biscaino Alves, Ivi Juliana Bristot, Rita Mattiello, Cláudio Corá Mottin, Fábio Klamt, Marcus Herbert Jones, Alexandre Vontobel Padoin, Fátima Costa Rodrigues Guma, Florencia María Barbé-Tuana
OBJECTIVE: To evaluate the consequences of plasma from individuals with obesity on parameters associated with immunosenescence in unrelated healthy peripheral blood mononuclear cells (PBMC). METHODS: Freshly isolated PBMC were incubated in media supplemented with 10% of plasma from individuals with obesity or control subjects for the first 4 hours of 24 to 120 hours of culture. RESULTS: Plasma from individuals with obesity modulated the phenotype of healthy PBMC, leading to a higher rate of apoptosis, lower amounts of phospho-γH2AX and -p53, and mitochondrial dysfunction...
July 14, 2017: Obesity
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