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https://www.readbyqxmd.com/read/29323143/optimizing-human-treg-immunotherapy-by-treg-subset-selection-and-e-selectin-ligand-expression
#1
Conor Donnelly, Brad Dykstra, Nandini Mondal, Junning Huang, Belinda J Kaskow, Russell Griffin, Robert Sackstein, Clare Baecher-Allan
While human Tregs hold immense promise for immunotherapy, their biologic variability poses challenges for clinical use. Here, we examined clinically-relevant activities of defined subsets of freshly-isolated and culture-expanded human PBMC-derived Tregs. Unlike highly suppressive but plastic memory Tregs (memTreg), naïve Tregs (nvTreg) exhibited the greatest proliferation, suppressive capacity after stimulation, and Treg lineage fidelity. Yet, unlike memTregs, nvTregs lack Fucosyltransferase VII and display low sLeX expression, with concomitant poor homing capacity...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29316067/deep-sequencing-shows-low-level-oncogenic-hepatitis-b-virus-variants-persisting-post-liver-transplant-despite-potent-anti-hbv-prophylaxis
#2
Keith Ck Lau, Carla Osiowy, Elizabeth Giles, Beth Lusina, Guido van Marle, Kelly W Burak, Carla S Coffin
Recent studies suggest that withdrawal of hepatitis B immune globulin (HBIG) and nucleos(t)ide analogues (NA) prophylaxis may be considered in HBV surface antigen (HBsAg) negative liver transplant (LT) recipients with a low risk of disease recurrence. However, the frequency of occult HBV infection (OBI) and HBV variants after LT in the current era of potent NA therapy is unknown. 12 LT recipients on prophylaxis were tested in matched plasma and peripheral blood mononuclear cells (PBMC) for HBV quasispecies by in-house nested PCR and next generation sequencing of amplicons...
January 6, 2018: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/29281498/continuous-and-quantitative-purification-of-t-cell-subsets-for-cell-therapy-manufacturing-using-magnetic-ratcheting-cytometry
#3
Coleman Murray, Edward Pao, Andrew Jann, Da Eun Park, Dino Di Carlo
T-cell-based immunotherapies represent a growing medical paradigm that has the potential to revolutionize contemporary cancer treatments. However, manufacturing bottlenecks related to the enrichment of therapeutically optimal T-cell subpopulations from leukopak samples impede scale-up and scale-out efforts. This is mainly attributed to the challenges that current cell purification platforms face in balancing the quantitative sorting capacity needed to isolate specific T-cell subsets with the scalability to meet manufacturing throughputs...
December 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/29278294/chemotherapy-immunophenoprofiles-in-non-small-cell-lung-cancer-by-personalized-membrane-proteomics
#4
Denise Utami Putri, Po-Hao Feng, Yuu-Hueih Hsu, Kang-Yun Lee, Feng-Wen Jiang, Lu-Wei Kuo, Yu-Ju Chen, Chia-Li Han
OBJECTIVES: No study has addressed how the immune status at the molecular level is affected by first-line pemetrexed and cisplatin (PEM-CIS) combination therapy in patients with non-small-cell lung cancer (NSCLC). Thus, we aimed to identify the immune status from membrane proteome alterations in patients with NSCLC upon PEM-CIS treatment. METHODS: The paired peripheral blood mononuclear cells (PBMCs) were collected from four patients with lung adenocarcinoma before and after the first regimen of PEM-CIS treatment and applied quantitative membrane proteomics analysis...
December 26, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29277764/distribution-of-curcumin-and-thc-in-peripheral-blood-mononuclear-cells-isolated-from-healthy-individuals-and-patients-with-chronic-lymphocytic-leukemia
#5
Gordon T Bolger, Albert Licollari, Aimin Tan, Richard Greil, Lisa Pleyer, Brigitta Vcelar, Muhammad Majeed, Peter Sordillo
Background/Aim: Curcumin is being widely investigated for its anticancer properties and studies in the literature suggest that curcumin distributes to a higher degree in tumor versus non-tumor cells. In the current study, we report on investigation of the distribution of curcumin and metabolism to THC in PBMC from healthy individuals and chronic lymphocytic leukemia (CLL) patients following exposure to Lipocurc™ (liposomal curcumin). Materials and Methods: The time and temperature-dependent distribution of liposomal curcumin and metabolism to tetrahydrocurcumin (THC) were measured in vitro in human peripheral blood mononuclear cells (PBMC) obtained from healthy individuals, PBMCHI(cryopreserved and freshly isolated PBMC) and CLL patients (cryopreserved PBMC) with lymphocyte counts ranging from 17-58×106 cells/ml (PBMCCLL,Grp 1) and >150×106 cells/ml (PBMCCLL,Grp 2)...
January 2018: Anticancer Research
https://www.readbyqxmd.com/read/29248729/a-novel-3d-heterotypic-spheroid-model-for-studying-extracellular-vesicle-mediated-tumour-and-immune-cell-communication
#6
Lilite Sadovska, Elīna Zandberga, Krizia Sagini, Kaspars Jēkabsons, Una Riekstiņa, Zane Kalniņa, Alicia Llorente, Aija Linē
Cancer-derived extracellular vesicles (EVs) have emerged as important mediators of tumour-host interactions, and they have been shown to exert various functional effects in immune cells. In most of the studies on human immune cells, EVs have been isolated from cancer cell culture medium or patients' body fluids and added to the immune cell cultures. In such a setting, the physiological relevance of the chosen EV concentration is unknown and the EV isolation method and the timing of EV administration may bias the results...
December 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29237833/neutralizing-activity-of-broadly-neutralizing-anti-hiv-1-antibodies-against-clade-b-clinical-isolates-produced-in-peripheral-blood-mononuclear-cells
#7
Yehuda Z Cohen, Julio C C Lorenzi, Michael S Seaman, Lilian Nogueira, Till Schoofs, Lisa Krassnig, Allison Butler, Katrina Millard, Tomas Fitzsimons, Xiaoju Daniell, Juan P Dizon, Irina Shimeliovich, David C Montefiori, Marina Caskey, Michel C Nussenzweig
Recently discovered broadly neutralizing anti-HIV-1 antibodies (bNAbs) demonstrate extensive breadth and potency against diverse HIV-1 strains, and represent a promising approach for the treatment and prevention of HIV-1 infection. The breadth and potency of these antibodies have primarily been evaluated using panels of HIV-1 Env-pseudotyped viruses produced in 293T cells expressing molecularly cloned Envs. Here we report on the ability of 5 bNAbs currently in clinical development to neutralize circulating primary HIV-1 isolates derived from peripheral blood mononuclear cells (PBMC), and compare the results to the pseudovirus panels used to characterize the bNAbs...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29237049/the-comet-assay-in-human-biomonitoring-cryopreservation-of-whole-blood-and-comparison-with-isolated-mononuclear-cells
#8
Gudrun Koppen, Sofie De Prins, An Jacobs, Vera Nelen, Greet Schoeters, Sabine A S Langie
The comet assay is often applied in human biomonitoring. Most of the time the assay is performed with isolated peripheral blood mononuclear cells (PBMC). However, using whole blood instead of isolated cells reduces processing time, and only 20 µl is sufficient for analysis. In this study, a cryopreservation protocol for human whole blood for application in the comet assay was optimised by removing excess plasma before adding freezing medium. Cryopreservation of whole blood samples (n = 30) did not increase the detected level of strand breaks and formamidopyrimidine DNA glycosylase (FPG)-sensitive sites...
December 9, 2017: Mutagenesis
https://www.readbyqxmd.com/read/29236355/b-cell-specific-protein-fcrla-is-expressed-by-plasmacytoid-dendritic-cells-in-humans
#9
Evdokiya Reshetnikova, Sergey Guselnikov, Olga Volkova, Konstantin Baranov, Alexander Taranin, Ludmila Mechetina
BACKGROUND: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). METHODS: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers...
December 13, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29232525/a-modified-ficoll-paque-gradient-method-for-isolating-mononuclear-cells-from-the-peripheral-and-umbilical-cord-blood-of-humans-for-biobanks-and-clinical-laboratories
#10
Yanjuan Jia, Hui Xu, Yonghong Li, Chaojun Wei, Rui Guo, Fang Wang, Yu Wu, Jing Liu, Jing Jia, Junwen Yan, Xiaoming Qi, Yuanting Li, Xiaoling Gao
Although the Ficoll-Paque method is classically used to isolate peripheral blood mononuclear cells (PBMCs), modifications in this method are required for a more rapid and economic output for biobanks and clinical laboratories, particularly in developing countries. In this study, we addressed this issue by modifying the Ficoll-Paque method for the isolation of PBMCs or mononuclear cells from the peripheral and the umbilical cord blood of healthy and diseased (infected, anemic, and chronic obstructive pulmonary disease) adult individuals...
December 12, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/29227276/alteration-in-the-cytokine-secretion-pattern-in-t-cells-of-patients-with-cystic-fibrosis-caused-by-dna-methyltransferase-inhibitor-5-azacitidine
#11
E Kvaratskhelia, N Dabrundashvili, M Gagua, E Maisuradze, M Kamkamidze, E Abzianidze
Cystic fibrosis (CF) is the autosomal-recessive disorder caused by mutation in the cystic fibrosis transmembrane conductance regulator gene (CFTR). The Airway inflammation plays a central role in the progression of CF disease. Cystic fibrosis characterized by the overproduction of the pro-inflammatory cytokines and reduced expression of anti-inflammatory cytokines. Although the mechanisms of abnormal cytokine expression is still poorly understood, altered epigenetic regulations in T cells might contribute. In the present study we examined the expression of IFN-γ and IL-10 by CF T cells prior to and following 5-azaC treatment...
November 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29225295/effect-of-smoking-status-on-monocyte-tissue-factor-activity-carotid-atherosclerosis-and-long-term-prognosis-in-metabolic-syndrome
#12
Keiichi Kohashi, Akihiro Nakagomi, Taichirou Morisawa, Ikuko Endoh, Naomi Kawaguchi, Yoshiki Kusama, Wataru Shimizu
BACKGROUND: Smoking increases the risk of atherothrombotic events. Tissue factor (TF) mainly expressed on monocytes plays an important role in thrombosis and atherosclerosis. Metabolic syndrome (MetS) is being increasingly recognized as a major atherothrombotic risk factor, but the effects of smoking on monocyte TF activity (MTFA), carotid atherosclerosis estimated on carotid intima-media thickness (CIMT), and long-term prognosis in MetS remain unclear.Methods and Results:A total of 301 MetS patients lacking any known cardiovascular disease were prospectively investigated and classified into 4 groups according to smoking status at entry and at 12 months as follows: never smokers, past smokers, quitters, and persistent smokers...
December 9, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/29212911/a-role-of-nkr-p1a-cd161-and-lectin-like-transcript-1-in-natural-cytotoxicity-against-human-articular-chondrocytes
#13
Agata Białoszewska, Joanna Olkowska-Truchanowicz, Katarzyna Bocian, Anna Osiecka-Iwan, Andrzej Czop, Claudine Kieda, Jacek Malejczyk
Normal cartilage cells are susceptible to lysis by NK cells. This phenomenon may play a role in immune cartilage destruction; however, the mechanisms of chondrocyte recognition by NK cells remain poorly understood. Therefore, the aim of this study was to reveal a possible role of NKR-P1A/lectin-like transcript 1 (LLT1) interaction in NK cell-mediated cytotoxicity against normal human articular chondrocytes. Chondrocytes were isolated from articular cartilage obtained during talonavicular joint surgery. PBMC or polyclonal NK cells isolated from normal donors served as effector cells...
December 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29194121/hiv-infected-cannabis-users-have-lower-circulating-cd16-monocytes-and-ip-10-levels-compared-to-non-using-hiv-patients
#14
Michael D Rizzo, Robert B Crawford, Joseph E Henriquez, Yasser Aldhamen, Peter Gulick, Andrea Amalfitano, Norbert E Kaminski
OBJECTIVE: Chronic immune activation and elevated numbers of circulating activated monocytes (CD16) are implicated in HIV-associated neuroinflammation. The objective was to compare the level of circulating CD16 monocytes and interferon-γ-inducible protein 10 (IP-10) between HIV-infected cannabis users (HIV+MJ+) and non-cannabis users (HIV+MJ-), and determine whether in vitro Δ-Tetrahydrocannabinol (THC), a constituent of cannabis, affected CD16 expression as well as IP-10 production by monocytes...
November 30, 2017: AIDS
https://www.readbyqxmd.com/read/29182335/the-proteomic-landscape-of-patient-derived-cd4-t-cells-in-recent-onset-type-1-diabetes
#15
Marlen F Lepper, Uli Ohmayer, Christine von Toerne, Nicole Maison, Anette-Gabriele Ziegler, Stefanie M Hauck
The pathophysiology underlying the autoimmune disease type 1 diabetes (T1D) is poorly understood. Obtaining an accurate proteomic profile of the T helper cell population is essential for understanding the pathogenesis of T1D. Here, we performed in-depth proteomic profiling of peripheral CD4+ T cells in a pediatric cohort in order to identify cellular signatures associated with the onset of T1D. Using only 250,000 CD4+ T cells per patient, isolated from biobanked PBMC samples, we identified nearly 6,000 proteins using deep-proteome profiling with LC-MS/MS data-independent acquisition...
November 28, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29178041/cellular-immune-response-in-mdr-tb-patients-to-different-protein-expression-of-mdr-and-susceptible-mycobacterium-tuberculosis-rv0147-a-novel-mdr-tb-biomarker
#16
Alireza Hadizadeh Tasbiti, Shamsi Yari, Seyed Davar Siadat, Payam Tabarsi, Kayvan Saeedfar, Fatemeh Yari
Tuberculosis (TB) is a crucial public health problem with prevalence of multidrug resistant (MDR) rising. An accurate TB biomarker is urgently needed to monitor the response to treatment in patients with MDR tuberculosis. To analyze interaction between selected MDR-TB purified protein and immune cells, dendritic cells from MDR-TB patients and healthy subjects were stimulated by 55KDa protein fractions (Rv0147). The purified proteins identified by proteomic techniques (two-dimensional gel electrophoresis, mass spectrometry) and peptide sequences are known to bind a MHC class I alleles which are extracted from the Immune Epitope Database and Analysis Resource database ( www...
November 24, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29175653/igm-antibodies-against-phosphorylcholine-promote-polarization-of-t-regulatory-cells-from-patients-with-atherosclerotic-plaques-systemic-lupus-erythematosus-and-healthy-donors
#17
Jitong Sun, Susanna L Lundström, Bo Zhang, Roman A Zubarev, Johnny Steuer, Peter Gillgren, Mizanur Rahman, Sofia Ajeganova, Anquan Liu, Johan Frostegård
BACKGROUND AND AIMS: IgM antibodies against phosphorylcholine (anti-PC) are negatively associated with atherosclerosis, cardiovascular disease (CVD) and systemic lupus erythematosus (SLE), where the risk of CVD and atherosclerosis is high. We here study the effects of IgM anti-PC immune regulation. METHODS: Mononuclear leukocytes were isolated from peripheral blood (PBMC) obtained from healthy blood donors, six SLE patients with age- and sex-matched controls, and symptom-giving human atherosclerotic plaques...
November 16, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/29174150/short-communication-decreasing-the-dietary-ratio-of-omega-6-to-omega-3-fatty-acids-increases-the-omega-3-concentration-of-peripheral-blood-mononuclear-cells-in-weaned-holstein-heifer-calves
#18
L C Worden, M G Erickson, S Gramer, C Tap, C Ylioja, N Trottier, C L Preseault, M J VandeHaar, A L Lock, E L Karcher
Utilization of nutrients to improve overall heifer health is of interest because of the importance of replacement heifers to the dairy industry. The objective of our study was to compare the effect of supplementation of dietary n-3 and n-6 fatty acids (FA) on FA concentrations in peripheral blood mononuclear cells (PBMC) of Holstein calves. Twenty-seven Holstein heifer calves (107 ± 2.6 d of age; 142.6 ± 6.5 kg of body weight) from the university research and teaching herd were randomly assigned to a common TMR supplemented with 1 of 3 treatments: Ca salts of flaxseed FA (Virtus Nutrition, Corcoran, CA) containing 35% 18:3 n-3 (N3), Ca salts of soybean FA (Virtus Nutrition) containing 50% 18:2 n-6 (N6), or a 50:50 mix of N3 and N6...
November 22, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29165554/the-effect-of-1-5%C3%A2-t-cardiac-magnetic-resonance-on-human-circulating-leucocytes
#19
William R Critchley, Anna Reid, Julie Morris, Josephine H Naish, John P Stone, Alexandra L Ball, Triin Major, David Clark, Nick Waldron, Christien Fortune, Jakub Lagan, Gavin A Lewis, Mark Ainslie, Erik B Schelbert, Daniel M Davis, Matthias Schmitt, James E Fildes, Christopher A Miller
Aims: Investigators have proposed that cardiovascular magnetic resonance (CMR) should have restrictions similar to those of ionizing imaging techniques. We aimed to investigate the acute effect of 1.5 T CMR on leucocyte DNA integrity, cell counts, and function in vitro, and in a large cohort of patients in vivo. Methods and results: In vitro study: peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers, and histone H2AX phosphorylation (γ-H2AX) expression, leucocyte counts, and functional parameters were quantified using flow cytometry under the following conditions: (i) immediately following PBMC isolation, (ii) after standing on the benchside as a temperature and time control, (iii) after a standard CMR scan...
November 18, 2017: European Heart Journal
https://www.readbyqxmd.com/read/29159986/l-asparaginase-isolated-from-streptomyces-ansochromogenes-promotes-th1-profile-and-activates-cd8-t-cells-in-human-pbmc-an-in-vitro-investigation
#20
Glêzia Renata da Silva Lacerda, Cristiane Moutinho Lagos de Melo, Ana Karine de Araújo Soares, Leyllane Rafael Moreira, Marília Cavalcanti Coriolano, Gláucia Manoella de Souza Lima, Thiago Henrique Napoleão, Virgínia Maria Barros de Lorena, Leonor Alves de Oliveira da Silva, Silene Carneiro do Nascimento
AIMS: A new L-asparaginase produced by Streptomyces ansochromogenes UFPEDA 3420 actinobacteria was used in this study against human lymphocyte cultures to evaluate the immunological profile induced by this enzyme. METHODS AND RESULTS: Cultures of lymphocytes were stimulated with S. ansochromogenes L-asparaginase and cytotoxicity, cell viability, cell stimulation and cytokine production were analyzed. This new S. ansochromogenes L-asparaginase induced activation and proliferation of the TCD8(+) lymphocyte subset and produced higher TNF-α, IFN-γ, IL-2 and IL-10 levels in a 24 hour assay...
November 21, 2017: Journal of Applied Microbiology
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