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https://www.readbyqxmd.com/read/27902371/mapping-b-lymphocytes-as-major-reservoirs-of-naturally-occurring-latent-equine-herpesvirus-5-infection
#1
Zelalem Mekuria, Charles El Hage, Nino P Ficorilli, Elizabeth A Washington, James R Gilkerson, Carol Hartley
Equine herpesvirus 5 (EHV5) is a commonly detected gammaherpesvirus which, along with the closely related EHV2, constitute the only two known Percaviruses that infect horses. Apart from detection in horse populations worldwide and the recent publication of the whole genome, there is little known about the biology and pathogenesis of this virus, with many assumptions made by parallels with EHV2. The long-term survival of gammaherpesviruses within infected hosts involves the establishment and maintenance of latency in selected cell and tissues types, particularly lymphocytes...
November 30, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27888161/sensitization-of-immune-cells-following-hexylaminolevulinate-photodynamic-therapy-of-cervical-intraepithelial-neoplasia
#2
Ralf Hass, Roland Jacobs, Andreas M Kaufmann, Peter Hillemanns, Philipp Soergel
BACKGROUND: Effects of photodynamic therapy (PDT) were tested with respect to immune cell stimulation in cervical intraepithelial neoplasia (CIN). METHODS: A patient with CIN received hexaminolevulinate (HAL) and subsequent PDT. These data were compared to a placebo PDT patient and a healthy HPV16-vaccinated donor. Isolation of peripheral blood mononuclear cells (PBMC) was performed before PDT and at 4 different time points after PDT. The proliferation of these PBMC was tested by [(3)H]thymidine incorporation following stimulation with control or HPV16-L1 peptides...
November 22, 2016: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/27881604/cd11d-%C3%AE-2-integrin-expression-on-human-nk-b-and-%C3%AE-%C3%AE-t-cells
#3
Gabrielle M Siegers, Christy R Barreira, Lynne-Marie Postovit, Gregory A Dekaban
The CD11d integrin is expressed on the cell surface of leukocytes that belong to the myeloid lineage, but its expression on lymphocytes remains unexplored. To test the hypothesis that CD11d is expressed on lymphocyte subsets, we employed a multicolor flow cytometry panel to identify CD11d expression on B, NK, CD4(+) and CD8(+) αβ T cells (αβTc), and γδ T cells (γδTc) in human PBMC samples. CD11d was highly expressed on NK cells, B cells, and γδTc, but not αβTc. CD11d expression was higher on freshly isolated γδTc compared with αβTc from healthy donors, yet both inter- and intradonor variability was evident...
November 23, 2016: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/27873461/human-peripheral-blood-mononuclear-cell-in-vitro-system-to-test-the-efficacy-of-food-bioactive-compounds-effects-of-polyunsaturated-fatty-acids-and-their-relation-with-bmi
#4
Margalida Cifre, Rubén Díaz-Rúa, Rubén Varela-Calviño, Bàrbara Reynés, Jordi Pericás-Beltrán, Andreu Palou, Paula Oliver
SCOPE: To analyse usefulness of isolated human peripheral blood mononuclear cells (PBMC) to rapidly/easily reflect n-3 long-chain polyunsaturated fatty acid (LCPUFA) effects on lipid metabolism/inflammation gene profile, and evaluate if these effects are BMI-dependent. METHODS AND RESULTS: PBMC from normoweight (NW) and overweight/obese (OW/OB) subjects were incubated with physiological doses of docosahexaenoic (DHA), eicosapentaenoic acid (EPA), or their combination...
November 22, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27868445/acetone-and-methanol-fruit-extracts-of-terminalia-paniculata-inhibit-hiv-1-infection-in-vitro
#5
Ankita Durge, Pratiksha Jadaun, Ashish Wadhwani, Ashish A Chinchansure, Madhukar Said, H V Thulasiram, Swati P Joshi, Smita S Kulkarni
In this study, we report the in vitro anti-HIV1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC50 values of both the extracts were ≥260 μg/mL. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D) and HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen...
November 20, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27857867/dna-methylation-signature-of-post-injury-neointimal-cells-during-vascular-remodeling-in-the-rat-balloon-injury-model
#6
Jendai Richards, Henry Ato Ogoe, Wenzhi Li, Oguljahan Babayewa, Wei Xu, Tameka Bythwood, Minerva Garcia-Barrios, Li Ma, Qing Song
Vascular smooth muscle cell (VSMC) accumulation in the neointimal is a common feature in vascular diseases such as atherosclerosis, transplant arteriosclerosis and restenosis. In this study, we isolated the neointimal cells and uninjured residential vascular smooth muscle cells by laser micro dissection and carried out single-cell whole-genome methylation sequencing. We also sequenced the bisulfite converted genome of circulating bone-marrow-derived cells such as peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC)...
July 2016: Molecular Biology (Los Angeles, Calif.)
https://www.readbyqxmd.com/read/27813153/traf6-mediates-suppression-of-osteoclastogenesis-and-prevention-of-ovariectomy-induced-bone-loss-by-a-novel-prenylflavonoid
#7
E M Tan, L Li, I R Indran, N Chew, E L Yong
Given the limitations of current therapeutic options for postmenopausal osteoporosis, there is a need for alternatives with minimal adverse effects. In this study, we evaluated the effects of icaritin (ICT), a natural prenylflavonoid, on osteoclastogenesis both in vitro and in an ovariectomized (OVX)-rat model, and investigated its underlying molecular mechanism(s) of action. ICT inhibited osteoclast formation in two osteoclast precursor models, RAW 264.7 mouse monocyte cell line and human PBMC. ICT also inhibited sealing zone and resorption pit formation in a dose-dependent manner...
November 4, 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/27809916/immunological-profiling-of-patients-with-ulcerative-colitis-leads-to-identification-of-two-inflammatory-conditions-and-cd1a-as-a-disease-marker
#8
M Föhlinger, P Palamides, U Mansmann, F Beigel, M Siebeck, R Gropp
BACKGROUND: Conventional approaches to understand mechanisms underlying the development of pathological manifestations in ulcerative colitis (UC) mostly rely on identification of certain cell types and cytokines followed by verification of their roles in vitro and in vivo. In light of the highly dynamic processes in UC, requiring the cross talk of immune cells, epithelial-, endothelial-, muscle cells and fibrocytes, this approach might neglect temporal and spatial connectivity of individually differing inflammatory responses...
November 3, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27798337/similar-to-those-who-are-breastfed-infants-fed-a-formula-containing-2-fucosyllactose-have-lower-inflammatory-cytokines-in-a-randomized-controlled-trial
#9
Karen C Goehring, Barbara J Marriage, Jeffery S Oliver, Julie A Wilder, Edward G Barrett, Rachael H Buck
BACKGROUND: Evidence suggests that human milk oligosaccharides (HMOs) provide multiple benefits to infants, including prebiotic effects, gut maturation, antimicrobial activities, and immune modulation. Clinical intervention studies with HMOs are required to confirm these benefits in infants. OBJECTIVE: Our objective was to investigate the effects of feeding formulas supplemented with the HMO 2'-fucosyllactose (2'-FL) on biomarkers of immune function in healthy term infants...
October 26, 2016: Journal of Nutrition
https://www.readbyqxmd.com/read/27797447/pyranochromones-from-dictyoloma-vandellianum-a-juss-and-their-cytotoxic-evaluation
#10
Iura M Alves, Lucas S Abreu, Cinara O S Costa, Mireille Le Hyaric, Maria Lenise S Guedes, Milena B P Soares, Daniel P Bezerra, Eudes S Velozo
One new chromone 3,3-dimethylallylspatheliachromene methyl ether 1, as well as five known chromones, 6-(3-methylbut-2-enyl) allopteroxylin methyl ether 2, 6-(3-methylbut-2-enyl) allopteroxylin 3, 3,3-dimethylallylspatheliachromene 4, 5-O-methylcneorumchromone K 5 and spatheliabischromen 6, two alkaloids, 8-methoxy-N-methylflindersine 7 and 8-methoxyflindersine 8, and two limonoids, limonin diosphenol 9 and rutaevin 10, were isolated from Dictyoloma vandellianum A. Juss (Rutaceae). Cytotoxic activities towards tumor cell lines B16-F10, HepG2, K562 and HL60 and non-tumor cells PBMC were evaluated for compounds 1-6...
October 31, 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/27795993/virtual-global-transplant-laboratory-standard-operating-procedures-for-blood-collection-pbmc-isolation-and-storage
#11
Lauren E Higdon, Karim Lee, Qizhi Tang, Jonathan S Maltzman
Research on human immune responses frequently involves the use of peripheral blood mononuclear cells (PBMC) immediately, or at significantly delayed timepoints, after collection. This requires PBMC isolation from whole blood and cryopreservation for some applications. It is important to standardize protocols for blood collection, PBMC isolation, cryopreservation, and thawing that maximize survival and functionality of PBMC at the time of analysis. This resource includes detailed protocols describing blood collection tubes, isolation of PBMC using a density gradient, cryopreservation of PBMC, and thawing of cells as well as preparation for functional assays...
September 2016: Transplantation Direct
https://www.readbyqxmd.com/read/27792414/toward-optimal-cryopreservation-and-storage-for-achievement-of-high-cell-recovery-and-maintenance-of-cell-viability-and-t-cell-functionality
#12
Stephanie Angel, Hagen von Briesen, Young-Joo Oh, Marko K Baller, Heiko Zimmermann, Anja Germann
Cryopreservation of biological materials such as cells, tissues, and organs is a prevailing topic of high importance. It is employed not only in many research fields but also in the clinical area. Cryopreservation is of great importance for reproductive medicine and clinical studies, as well as for the development of vaccines. Peripheral blood mononuclear cells (PBMCs) are commonly used in vaccine research where comparable and reliable results between different research institutions and laboratories are of high importance...
October 28, 2016: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/27731508/peripheral-blood-mononuclear-cells-infiltration-downregulates-decidual-faah-activity-in-an-lps-induced-embryo-resorption-model
#13
Manuel Luis Wolfson, Julieta Aisemberg, Fernando Correa, Ana María Franchi
Maternal infections with gram-negative bacteria are associated with miscarriage and are one of the most common complications during pregnancy. Previous studies from our group have shown that lipopolysaccharide (LPS)-activated infiltrating peripheral blood mononuclear cells (PBMC) into decidual tissue plays an important role in the establishment of a local inflammatory process that results in embryo cytotoxicity and early embryo resorption. Moreover, we have also shown that an increased endocannabinoid tone mediates LPS-induced deleterious effects during early pregnancy loss...
October 12, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27723787/clinically-relevant-reactivation-of-polyomavirus-bk-bkpyv-in-hla-a02-positive-renal-transplant-recipients-is-associated-with-impaired-effector-memory-differentiation-of-bkpyv-specific-cd8-t-cells
#14
Michiel C van Aalderen, Ester B M Remmerswaal, Kirstin M Heutinck, Anja Ten Brinke, Mariet C W Feltkamp, Neelke C van der Weerd, Karlijn A M I van der Pant, Frederike J Bemelman, René A W van Lier, Ineke J M Ten Berge
Polyomavirus BK (BKPyV) frequently reactivates in immunosuppressed renal transplant recipients (RTRs) and may lead to graft loss due to BKPyV-induced interstitial nephritis (BKVN). Little is known on the differentiation of CD8+ T cells targeting BKPyV in RTRs. Here we investigated whether BKPyV-specific CD8+ T cell differentiation differs in RTRs with varying degrees of BKPyV reactivation and/or BKVN. Using combinatorial encoding with tetramers carrying BKPyV major capsid protein (VP1) and large T antigen protein (LTAG) epitopes, we investigated CD8+ T cell responses to BKPyV in longitudinally obtained PBMC samples from 46 HLA-A02-positive RTRs and 20 healthy adults...
October 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27694998/glatiramer-acetate-treatment-normalized-the-monocyte-activation-profile-in-ms-patients-to-that-of-healthy-controls
#15
Delgertsetseg Chuluundorj, Scott A Harding, David Abernethy, Anne Camille La Flamme
Multiple sclerosis (MS) is an immune-mediated disease of the CNS, and monocytes contribute to MS-associated neuroinflammation. While classically-activated monocytes promote inflammation, type II-activated monocytes improve the course of MS. This study investigated type II activation of monocytes and their two main subsets, namely CD14(+) (CD14(++)CD16(-)subset) and CD16(+) monocytes (CD14(+)CD16(+)subset), by glatiramer acetate (GA) or intravenous immunoglobulin (IVIG)-associated immune complexes (IC), both of which are known MS treatments...
October 3, 2016: Immunology and Cell Biology
https://www.readbyqxmd.com/read/27688795/fungal-cell-wall-agents-and-bacterial-lipopolysaccharide-in-organic-dust-as-possible-risk-factors-for-pulmonary-sarcoidosis
#16
Sanja Stopinšek, Alojz Ihan, Barbara Salobir, Marjeta Terčelj, Saša Simčič
BACKGROUND: Composition of organic dust is very complex, involving particles of microbial, animal and plant origin. Several environmental exposure studies associate microbial cell wall agents in organic dust with various respiratory symptoms and diseases. The aim of the present study was to investigate the in vitro effects of the co-exposure of fungal cell wall agents (FCWAs) and bacterial lipopolysaccharide (LPS) on inflammatory immune responses of peripheral blood mononuclear cells (PBMCs) from patients with pulmonary sarcoidosis...
2016: Journal of Occupational Medicine and Toxicology
https://www.readbyqxmd.com/read/27620196/the-novelty-in-fabrication-of-poly-vinyl-alcohol-%C3%AE%C2%BA-carrageenan-hydrogel-with-lactobacillus-bulgaricus-extract-as-anti-inflammatory-wound-dressing-agent
#17
Gomaa F El-Fawal, Abdelrahman M Yassin, Nehal M El-Deeb
Material barrier properties to microbes are an important issue in many pharmaceutical applications like wound dressings. A wide range of biomaterials has been used to manage the chronic inflamed wounds. Eight hydrogel membranes of poly vinyl alcohol (PVA) with κ-carrageenan (KC) and Lactobacillus bulgaricus extract (LAB) have been prepared by using freeze-thawing technique. To evaluate the membranes efficiency as wound dressing agents, various tests have been done like gel fraction, swelling behavior, mechanical properties, etc...
September 12, 2016: AAPS PharmSciTech
https://www.readbyqxmd.com/read/27610159/potent-anti-inflammatory-activity-of-tetramethylpyrazine-is-mediated-through-suppression-of-nf-k
#18
Wei Chen, Weixiong Chen, Jinshui Zhu, Niwei Chen, Yunmin Lu
The purpose of the current study was to evaluate the anti-inflammatory activity of tetramethlpyrazine on oxazolone-induced colitis mice. Spleen mononuclear cells (SMC), lamina propria mononuclear cells (LPMC) and peripheral blood mononuclear cells (PBMC) were isolated from oxazolone-induced colitis and normal mice. The colitis cells treated by oxazolone were randomly divided into model, low dose, middle dose and high dose groups treated with 0, 0.5, 1.0 and 2.0 g/L tetramethlpyrazine, respectively. The apoptotic rate of SMC and LPMC in the oxazolone-induced group was lower than that in the normal group...
2016: Iranian Journal of Pharmaceutical Research: IJPR
https://www.readbyqxmd.com/read/27609280/dopaminergic-receptors-and-adrenoceptors-in-circulating-lymphocytes-as-putative-biomarkers-for-the-early-onset-and-progression-of-multiple-sclerosis
#19
Marco Cosentino, Mauro Zaffaroni, Massimiliano Legnaro, Raffaella Bombelli, Laura Schembri, Damiano Baroncini, Anna Bianchi, Raffaella Clerici, Mario Guidotti, Paola Banfi, Giorgio Bono, Franca Marino
Clinically isolated syndrome (CIS) is a first, usually recovering, episode of neurological disturbance(s) suggestive of multiple sclerosis (MS). CIS subjects might benefit from early disease-modifying drugs, provided that those at high risk of developing MS can be identified. Gene expression for dopaminergic receptors (DR) and adrenoceptors (AR) is dysregulated in lymphocytes of MS patients and is affected by treatment with interferon (IFN)-β. In particular, lymphocyte DR D5 mRNA might be a marker of IFN-β response in MS patients...
September 15, 2016: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/27583440/il-28b-genetic-variants-determine-the-extent-of-monocyte-induced-activation-of-nk-cells-in-hepatitis-c
#20
Benjamin Krämer, Claudia Finnemann, Beatriz Sastre, Philipp Lutz, Andreas Glässner, Franziska Wolter, Felix Goeser, Pavlos Kokordelis, Dominik Kaczmarek, Hans-Dieter Nischalke, Christian P Strassburg, Ulrich Spengler, Jacob Nattermann
BACKGROUND: Immuno-genetic studies suggest a functional link between NK cells and λ-IFNs. We recently showed that NK cells are negative for the IFN-λ receptor IFN-λR1 and do not respond to IFN-λ, suggesting a rather indirect association between IL-28B genotype and NK cell activity. METHODS: A total of 75 HCV(+) patients and 67 healthy controls were enrolled into this study. IL-28B (rs12979860) and IFNL-4 (rs368234815) genotypes were determined by rtPCR. Total PBMC, monocytes, and NK cells were stimulated with IL-29, the TLR-7/8 agonist R848, or a combination of both...
2016: PloS One
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