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https://www.readbyqxmd.com/read/28732136/an-approach-to-estimate-the-activation-energies-of-fragmentation-occurring-in-quadrupole-collision-cell-of-the-mass-spectrometer
#1
Ákos Kuki, Lajos Nagy, Miklós Zsuga, Sándor Kéki
The classical semi-quantitative Rice-Ramsperger-Kassel (RRK) theory was used for the calculation of the internal energy dependent reaction rate coefficient of the collision-induced dissociation (CID) reaction in tandem mass spectrometry (MS/MS). The survival yield (SY) was determined by the reaction rate equation for the unimolecular dissociation of the precursor ion. The parameters of the rate equation and the RRK model were approximated based on the instrumental conditions. We used the RRK equation for the description of the basic behavior of the fragmentation reactions and for the estimation of the internal energy of the precursor ion...
July 21, 2017: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/28731526/drug-discovery-and-development-for-rare-genetic-disorders
#2
REVIEW
Wei Sun, Wei Zheng, Anton Simeonov
Approximately 7,000 rare diseases affect millions of individuals in the United States. Although rare diseases taken together have an enormous impact, there is a significant gap between basic research and clinical interventions. Opportunities now exist to accelerate drug development for the treatment of rare diseases. Disease foundations and research centers worldwide focus on better understanding rare disorders. Here, the state-of-the-art drug discovery strategies for small molecules and biological approaches for orphan diseases are reviewed...
July 21, 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28730481/label-free-phosphoproteomic-approach-for-kinase-signaling-analysis
#3
Edmund Wilkes, Pedro R Cutillas
Phosphoproteomics is a powerful platform for the unbiased profiling of kinase-driven signaling pathways. Quantitation of phosphorylation can be performed by means of either labeling or label-free mass spectrometry (MS) methods. Because of their simplicity and universality, label-free methodology is gaining acceptance and popularity in molecular biology research. Analytical workflows for label-free quantification of phosphorylation, however, need to overcome several hurdles for the technique to be accurate and precise...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28729406/tumor-evolution-as-a-therapeutic-target
#4
REVIEW
Nabil Amirouchene-Angelozzi, Charles Swanton, Alberto Bardelli
Recent technological advances in the field of molecular diagnostics (including blood-based tumor genotyping) allow the measurement of clonal evolution in patients with cancer, thus adding a new dimension to precision medicine: time. The translation of this new knowledge into clinical benefit implies rethinking therapeutic strategies. In essence, it means considering as a target not only individual oncogenes but also the evolving nature of human tumors. Here, we analyze the limitations of targeted therapies and propose approaches for treatment within an evolutionary framework...
July 20, 2017: Cancer Discovery
https://www.readbyqxmd.com/read/28729110/no-211-screening-and-management-of-bacterial-vaginosis-in-pregnancy
#5
Mark H Yudin, Deborah M Money
OBJECTIVE: To review the evidence and provide recommendations on screening for and management of bacterial vaginosis in pregnancy. OPTIONS: The clinical practice options considered in formulating the guideline. OUTCOMES: Outcomes evaluated include antibiotic treatment efficacy and cure rates, and the influence of the treatment of bacterial vaginosis on the rates of adverse pregnancy outcomes such as preterm labour and delivery and preterm premature rupture of membranes...
August 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28727998/enrichment-of-endogenous-fractalkine-and-anti-inflammatory-cells-via-aptamer-functionalized-hydrogels
#6
Syed Faaiz Enam, Jack R Krieger, Tarun Saxena, Brian E Watts, Claire E Olingy, Edward A Botchwey, Ravi V Bellamkonda
Early recruitment of non-classical monocytes and their macrophage derivatives is associated with augmented tissue repair and improved integration of biomaterial constructs. A promising therapeutic approach to recruit these subpopulations is by elevating local concentrations of chemoattractants such as fractalkine (FKN, CX3CL1). However, delivering recombinant or purified proteins is not ideal due to their short half-lives, suboptimal efficacy, immunogenic potential, batch variabilities, and cost. Here we report an approach to enrich endogenous FKN, obviating the need for delivery of exogenous proteins...
July 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/28727567/intuitionistic-multiplicative-group-analytic-hierarchy-process-and-its-use-in-multicriteria-group-decision-making
#7
Zhiming Zhang, Witold Pedrycz
As an extension of multiplicative preference relations (MPRs), intuitionistic MPRs (IMPRs) reflect experts' hesitant quantitative judgments. This paper presents an intuitionistic multiplicative preference information-based group analytic hierarchy process (AHP) and develops an intuitionistic multiplicative group AHP (IMGAHP), which addresses multicriteria group decision-making (MCGDM) that uses IMPRs to capture experts' preference judgments. First, we introduce a consistency index to gauge the consistency of IMPRs and describe the concept of acceptably consistent IMPRs...
July 17, 2017: IEEE Transactions on Cybernetics
https://www.readbyqxmd.com/read/28726919/exploring-the-construction-of-multicompartmental-micelles-by-halogen-bonding-of-complementary-macromolecules
#8
Alan Vanderkooy, Mark S Taylor
An approach to the construction of multicompartmental micelles, using halogen bonding between complementary macromolecules, is described. The design involves a sequential assembly protocol, in which the initial compartments are formed by interpolymer halogen bonding, followed by the collapse of a second, hydrophobic compartment upon transfer to aqueous solvent. Triblock terpolymers incorporating a halogen bond accepting segment have been synthesized. Transmission electron microscopy was used to characterize multicompartmental assemblies generated from these terpolymers in the presence of a halogen bond donor-functionalized polystyrene derivative...
July 20, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28726724/theoretical-studies-on-two-photon-fluorescent-hg-2-probes-based-on-the-coumarin-rhodamine-system
#9
Yujin Zhang, Jiancai Leng
The development of fluorescent sensors for Hg(2+) has attracted much attention due to the well-known adverse effects of mercury on biological health. In the present work, the optical properties of two newly-synthesized Hg(2+) chemosensors based on the coumarin-rhodamine system (named Pro1 and Pro2) were systematically investigated using time-dependent density functional theory. It is shown that Pro1 and Pro2 are effective ratiometric fluorescent Hg(2+) probes, which recognize Hg(2+) by Förster resonance energy transfer and through bond energy transfer mechanisms, respectively...
July 20, 2017: Sensors
https://www.readbyqxmd.com/read/28726180/epr-characterization-of-dinitrosyl-iron-complexes-with-thiol-containing-ligands-as-an-approach-to-their-identification-in-biological-objects-an-overview
#10
REVIEW
Anatoly F Vanin
The overview demonstrates how the use of only one physico-chemical approach, viz., the electron paramagnetic resonance method, allowed detection and identification of dinitrosyl iron complexes with thiol-containing ligands in various animal and bacterial cells. These complexes are formed in biological objects in the paramagnetic (electron paramagnetic resonance-active) mononuclear and diamagnetic (electron paramagnetic resonance-silent) binuclear forms and control the activity of nitrogen monoxide, one of the most universal regulators of metabolic processes in the organism...
July 19, 2017: Cell Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28724728/global-decline-of-bumblebees-is-phylogenetically-structured-and-inversely-related-to-species-range-size-and-pathogen-incidence
#11
Marina P Arbetman, Gabriela Gleiser, Carolina L Morales, Paul Williams, Marcelo A Aizen
Conservation biology can profit greatly from incorporating a phylogenetic perspective into analyses of patterns and drivers of species extinction risk. We applied such an approach to analyse patterns of bumblebee (Bombus) decline. We assembled a database representing approximately 43% of the circa 260 globally known species, which included species extinction risk assessments following the International Union fo Conservation of Nature Red List categories and criteria, and information on species traits presumably associated with bumblebee decline...
July 26, 2017: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/28720872/associating-transcription-factors-and-conserved-rna-structures-with-gene-regulation-in-the-human-brain
#12
Nikolai Hecker, Stefan E Seemann, Asli Silahtaroglu, Walter L Ruzzo, Jan Gorodkin
Anatomical subdivisions of the human brain can be associated with different neuronal functions. This functional diversification is reflected by differences in gene expression. By analyzing post-mortem gene expression data from the Allen Brain Atlas, we investigated the impact of transcription factors (TF) and RNA secondary structures on the regulation of gene expression in the human brain. First, we modeled the expression of a gene as a linear combination of the expression of TFs. We devised an approach to select robust TF-gene interactions and to determine localized contributions to gene expression of TFs...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28720856/optimization-of-microfluidic-biosensor-efficiency-by-means-of-fluid-flow-engineering
#13
Marwa Selmi, Mohamed Hichem Gazzah, Hafedh Belmabrouk
Binding reaction kinetics of analyte-ligand at the level of a sensitive membrane into a microchannel of a biosensor has been limited by the formation of the boundary diffusion layer. Therefore, the response time increases and affects the overall performance of a biosensor. In the present work, we develop an approach to engineer fluid streams into a complex configuration in order to improve the binding efficiency. We investigate numerically the flow deformations around a parallelepiped with square cross-section inside the microfluidic channel and exploit these deformations to simulate the analyte transport to the sensitive membrane and enhance both association and dissociation processes...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28719201/silver-catalyzed-cascade-1-6-addition-cyclization-of-para-quinone-methides-with-propargyl-malonates-an-approach-to-spiro-4-5-deca-6-9-dien-8-ones
#14
Zhenbo Yuan, Lina Liu, Rui Pan, Hequan Yao, Aijun Lin
An unprecedented silver-catalyzed cascade 1,6-addition/5-exo-dig cyclization reaction between para-quinone methides and propargyl malonates under mild reaction conditions has been described. This reaction provides an efficient method to construct versatile spiro[4.5]cyclohexadienones in moderate to excellent yields with high atom economy and scalability.
July 18, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28716787/can-nurse-teachers-manage-student-incivility-by-guided-democracy-a-grounded-theory-study
#15
Mostafa Rad, Hossein Karimi Moonaghi, Eshagh Ildarabadi
INTRODUCTION: Managing incivility in academic settings is among the basic concerns and challenges of most educational systems, including nursing education. Incivility management cannot be considered devoid of disruptive behaviors. However, incivility management is a complexphenomenon upon which few studies are conducted. OBJECTIVES: The present study aims at discovering teachers and students' experiences regarding incivility and developing an approach to manage nursing students' incivility...
July 17, 2017: BMJ Open
https://www.readbyqxmd.com/read/28715105/a-simulation-based-approach-to-measuring-team-situational-awareness-in-emergency-medicine-a-multicenter-observational-study
#16
Elizabeth D Rosenman, Aurora J Dixon, Jessica M Webb, Sarah Brolliar, Simon J Golden, Kerin A Jones, Sachita Shah, James A Grand, Steve W J Kozlowski, Georgia T Chao, Rosemarie Fernandez
OBJECTIVES: Team situational awareness (TSA) is critical for effective teamwork and supports dynamic decision-making in unpredictable, time-pressured situations. Simulation provides a platform for developing and assessing TSA; but these efforts are limited by suboptimal measurement approaches. The objective of this study was to develop and evaluate a novel approach to TSA measurement in interprofessional emergency medicine teams. METHODS: We performed a multicenter, prospective, simulation-based observational study to evaluate an approach to TSA measurement...
July 17, 2017: Academic Emergency Medicine: Official Journal of the Society for Academic Emergency Medicine
https://www.readbyqxmd.com/read/28714990/an-approach-to-suppress-the-evolution-of-resistance-in-braf-v600e-mutant-cancer
#17
Yaohua Xue, Luciano Martelotto, Timour Baslan, Alberto Vides, Martha Solomon, Trang Thi Mai, Neelam Chaudhary, Greg J Riely, Bob T Li, Kerry Scott, Fabiola Cechhi, Ulrika Stierner, Kalyani Chadalavada, Elisa de Stanchina, Sarit Schwartz, Todd Hembrough, Gouri Nanjangud, Michael F Berger, Jonas Nilsson, Scott W Lowe, Jorge S Reis-Filho, Neal Rosen, Piro Lito
The principles that govern the evolution of tumors exposed to targeted therapy are poorly understood. Here we modeled the selection and propagation of an amplification in the BRAF oncogene (BRAF(amp)) in patient-derived tumor xenografts (PDXs) that were treated with a direct inhibitor of the kinase ERK, either alone or in combination with other ERK signaling inhibitors. Single-cell sequencing and multiplex fluorescence in situ hybridization analyses mapped the emergence of extra-chromosomal amplification in parallel evolutionary trajectories that arose in the same tumor shortly after treatment...
July 17, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28710404/assuring-quality-in-microplastic-monitoring-about-the-value-of-clean-air-devices-as-essentials-for-verified-data
#18
Charlotte Wesch, Anna Maria Elert, Manuel Wörner, Ulrike Braun, Roland Klein, Martin Paulus
Avoiding aerial microfibre contamination of environmental samples is essential for reliable analyses when it comes to the detection of ubiquitous microplastics. Almost all laboratories have contamination problems which are largely unavoidable without investments in clean-air devices. Therefore, our study supplies an approach to assess background microfibre contamination of samples in the laboratory under particle-free air conditions. We tested aerial contamination of samples indoor, in a mobile laboratory, within a laboratory fume hood and on a clean bench with particles filtration during the examining process of a fish...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28709313/emergence-of-robustness-in-networks-of-networks
#19
Kevin Roth, Flaviano Morone, Byungjoon Min, Hernán A Makse
A model of interdependent networks of networks (NONs) was introduced recently [Proc. Natl. Acad. Sci. (USA) 114, 3849 (2017)PNASA60027-842410.1073/pnas.1620808114] in the context of brain activation to identify the neural collective influencers in the brain NON. Here we investigate the emergence of robustness in such a model, and we develop an approach to derive an exact expression for the random percolation transition in Erdös-Rényi NONs of this kind. Analytical calculations are in agreement with numerical simulations, and highlight the robustness of the NON against random node failures, which thus presents a new robust universality class of NONs...
June 2017: Physical Review. E
https://www.readbyqxmd.com/read/28708857/comparative-transcriptome-analysis-of-flower-heterosis-in-two-soybean-f1-hybrids-by-rna-seq
#20
Chunbao Zhang, Chunjing Lin, Fuyou Fu, Xiaofang Zhong, Bao Peng, Hao Yan, Jingyong Zhang, Weilong Zhang, Pengnian Wang, Xiaoyang Ding, Wei Zhang, Limei Zhao
Heterosis has been widely exploited as an approach to enhance crop traits during breeding. However, its underlying molecular genetic mechanisms remain unclear. Recent advances in RNA sequencing technology (RNA-seq) have provided an opportunity to conduct transcriptome profiling for heterosis studies. We used RNA-seq to analyze the flower transcriptomes of two F1 hybrid soybeans (HYBSOY-1 and HYBSOY-5) and their parents. More than 385 million high-quality reads were generated and aligned against the soybean reference genome...
2017: PloS One
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