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https://www.readbyqxmd.com/read/27914838/lacrimal-gland-involvement-in-blepharophimosis-ptosis-epicanthus-inversus-syndrome
#1
Ana Filipa Duarte, Patricia M S Akaishi, Greice A de Molfetta, Salomão Chodraui-Filho, Murilo Cintra, Alcina Toscano, Wilson Araujo Silva, Antonio A V Cruz
PURPOSE: To describe the involvement of the lacrimal gland (LG) in blepharophimosis-ptosis-epicanthus inversus syndrome (BPES). DESIGN: Observational, cross-sectional study. PARTICIPANTS: Twenty-one patients with BPES (10 female, 11 male) aged on average 15 years (range, 2-39 years), from 3 Brazilian medical centers and 1 Portuguese medical center. METHODS: Patients had their ocular surface evaluated with slit-lamp biomicroscopy, and tear production quantified with the Schirmer test I...
November 30, 2016: Ophthalmology
https://www.readbyqxmd.com/read/27911924/plasmodium-yoelii-nigeriensis-n67-is-a-robust-animal-model-to-study-malaria-transmission-by-south-american-anopheline-mosquitoes
#2
Alessandra S Orfano, Ana Paula M Duarte, Alvaro Molina-Cruz, Paulo F Pimenta, Carolina Barillas-Mury
Malaria is endemic in the American continent and the Amazonian rainforest is the region with the highest risk of transmission. However, the lack of suitable experimental models to infect malaria vectors from the Americas has limited the progress to understand the biology of transmission in this region. Anopheles aquasalis, a major vector in coastal areas of South America, was found to be highly refractory to infection with two strains of Plasmodium falciparum (NF54 and 7G8) and with Plasmodium berghei (mouse malaria), even when the microbiota was eliminated with antibiotics and oxidative stress was reduced with uric acid...
2016: PloS One
https://www.readbyqxmd.com/read/27910309/spatial-resolution-measurements-of-the-advanced-radiographic-capability-x-ray-imaging-system-at-energies-relevant-to-compton-radiography
#3
G N Hall, N Izumi, O L Landen, R Tommasini, J P Holder, D Hargrove, D K Bradley, A Lumbard, J G Cruz, K Piston, J J Lee, E Romano, P M Bell, A C Carpenter, N E Palmer, B Felker, V Rekow, F V Allen
Compton radiography provides a means to measure the integrity, ρR and symmetry of the DT fuel in an inertial confinement fusion implosion near peak compression. Upcoming experiments at the National Ignition Facility will use the ARC (Advanced Radiography Capability) laser to drive backlighter sources for Compton radiography experiments and will use the newly commissioned AXIS (ARC X-ray Imaging System) instrument as the detector. AXIS uses a dual-MCP (micro-channel plate) to provide gating and high DQE at the 40-200 keV x-ray range required for Compton radiography, but introduces many effects that contribute to the spatial resolution...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909741/the-role-of-epigenetic-modifiers-in-extended-cultures-of-functional-hepatocyte-like-cells-derived-from-human-neonatal-mesenchymal-stem-cells
#4
M Cipriano, J C Correia, S P Camões, N G Oliveira, P Cruz, H Cruz, M Castro, J L Ruas, J M Santos, J P Miranda
The development of predictive in vitro stem cell-derived hepatic models for toxicological drug screening is an increasingly important topic. Herein, umbilical cord tissue-derived mesenchymal stem cells (hnMSCs) underwent hepatic differentiation using an optimized three-step core protocol of 24 days that mimicked liver embryogenesis with further exposure to epigenetic markers, namely the histone deacetylase inhibitor trichostatin A (TSA), the cytidine analogue 5-azacytidine (5-AZA) and dimethyl sulfoxide (DMSO)...
December 1, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27908635/from-the-definition-to-the-social-impact-of-premature-ejaculation-expert-consensus
#5
EDITORIAL
N Cruz-Navarro, R Prieto-Castro, F Cabello-Santamaría, F García-José, A Fernández-Lozano, M Larrazábal-Murillo, A Martín-Morales, F Sánchez-Sánchez, C San Martín-Blanco, J Viladoms-Fuster
No abstract text is available yet for this article.
November 28, 2016: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/27908570/sleep-related-disorders-in-latin-american-children-with-atopic-dermatitis-a-case-control-study
#6
M Urrutia-Pereira, D Solé, N A Rosario, H J C Neto, V Acosta, C F Almendarez, M M Avalos, H Badellino, F Berroa, M Álvarez-Castelló, A J Castillo, R L Castro-Almarales, M M De la Cruz, A M Cepeda, C Fernandez, M González-León, J Lozano-Saenz, C Sanchez-Silot, J C Sisul-Alvariza, M Valentin-Rostan, R O S Sarni
BACKGROUND: Atopic dermatitis (AD) has been associated with impairment of sleep. The aim of this study was to evaluate sleep disorders in AD Latin-American children (4-10 years) from nine countries, and in normal controls (C). METHODS: Parents from 454 C and 340 AD children from referral clinics answered the Children Sleep Habits Questionnaire (CSHQ), a one-week retrospective 33 questions survey under seven items (bedtime resistance, sleep duration, sleep anxiety, night awakening, parasomnias, sleep-disordered breathing and daytime sleepiness)...
November 28, 2016: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/27907913/screening-younger-individuals-in-a-primary-care-setting-flags-putative-dementia-cases-and-correlates-gastrointestinal-diseases-with-poor-cognitive-performance
#7
Ilka M Rosa, Ana Gabriela Henriques, Liliana Carvalho, Joana Oliveira, Odete A B da Cruz E Silva
BACKGROUND/AIMS: Diagnosing dementia is challenging in many primary care settings, given the limited human resources and the lack of current diagnostic tools. With this in mind, a primary care-based cohort was established in the Aveiro district of Portugal. METHODS: A total of 568 participants were evaluated using cognitive tests and APOE genotyping. RESULTS: The findings revealed a dementia prevalence of 12%. A strong correlation between increasing Clinical Dementia Rating (CDR) scores and education was clearly evident...
December 2, 2016: Dementia and Geriatric Cognitive Disorders
https://www.readbyqxmd.com/read/27907136/immune-activation-and-bacterial-translocation-a-link-between-impaired-immune-recovery-and-frequent-visceral-leishmaniasis-relapses-in-hiv-infected-patients
#8
Maria Luciana Silva-Freitas, Glaucia Fernandes Cota, Talia S Machado-de-Assis, Carmem Giacoia-Gripp, Ana Rabello, Alda M Da-Cruz, Joanna R Santos-Oliveira
The maintenance of chronic immune activation due to leishmaniasis or even due to microbial translocation is associated with immunosenescence and may contribute to frequent relapses. Our aim was to investigate whether patients with HIV-associated visceral leishmaniasis (VL/HIV) who experience a single episode of VL have different immunological behaviors in comparison to those who experience frequent relapses. VL/HIV patients were allocated to non-relapsing (NR, n = 6) and relapsing (R, n = 11) groups and were followed from the active phase of VL up to 12 months post-treatment (mpt)...
2016: PloS One
https://www.readbyqxmd.com/read/27903492/angiotensinogen-import-in-isolated-proximal-tubules-evidence-for-mitochondrial-trafficking-and-uptake
#9
Bryan A Wilson, Nildris Cruz-Diaz, Yixin Su, James C Rose, TanYa M Gwathmey, Mark C Chappell
The renal proximal tubules are a key functional component of the kidney and express the angiotensin precursor angiotensinogen; however, it is unclear the extent that tubular angiotensinogen reflects local synthesis or internalization. Therefore, the current study established the extent that angiotensinogen is internalized by proximal tubules and the intracellular distribution. Proximal tubules were isolated from the kidney cortex of male sheep by enzymatic digestion and a discontinuous Percoll gradient. Tubules were incubated with radiolabeled 125I-angiotensinogen for 2 hrs at 37°C in serum/phenol-free DMEM/F12 media...
November 30, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27899642/the-ucsc-genome-browser-database-2017-update
#10
Cath Tyner, Galt P Barber, Jonathan Casper, Hiram Clawson, Mark Diekhans, Christopher Eisenhart, Clayton M Fischer, David Gibson, Jairo Navarro Gonzalez, Luvina Guruvadoo, Maximilian Haeussler, Steve Heitner, Angie S Hinrichs, Donna Karolchik, Brian T Lee, Christopher M Lee, Parisa Nejad, Brian J Raney, Kate R Rosenbloom, Matthew L Speir, Chris Villarreal, John Vivian, Ann S Zweig, David Haussler, Robert M Kuhn, W James Kent
Since its 2001 debut, the University of California, Santa Cruz (UCSC) Genome Browser (http://genome.ucsc.edu/) team has provided continuous support to the international genomics and biomedical communities through a web-based, open source platform designed for the fast, scalable display of sequence alignments and annotations landscaped against a vast collection of quality reference genome assemblies. The browser's publicly accessible databases are the backbone of a rich, integrated bioinformatics tool suite that includes a graphical interface for data queries and downloads, alignment programs, command-line utilities and more...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27899452/structural-basis-of-the-oncogenic-interaction-of-phosphatase-prl-1-with-the-magnesium-transporter-cnnm2
#11
Paula Giménez-Mascarell, Iker Oyenarte, Serge Hardy, Tilman Breiderhoff, Marchel Stuiver, Elie Kostantin, Tammo Diercks, Angel L Pey, June Ereño-Orbea, María Luz Martinez-Chantar, Reham Khalaf-Nazzal, Félix Claverie-Martín, Dominik Müller, Michel L Tremblay, Luis Alfonso Martínez-Cruz
Phosphatases of regenerating liver (PRLs), the most oncogenic of all protein tyrosine phosphatases (PTPs), play a critical role in metastatic progression of cancers. Recent findings established a new paradigm by uncovering that their association with magnesium transporters of the Cyclin M (CNNM) family causes intracellular magnesium levels that promotes oncogenic transformation. On the other hand, it has recently been highlighted essential roles of the CNNM family in regulation of the circadian rhythm, and reproduction...
November 29, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27898875/relationships-of-feedlot-performance-feeding-behavior-rumen-morphometrics-and-carcass-characteristics-of-nellore-cattle-differing-in-phenotypic-residual-feed-intake
#12
M C S Pereira, G D Cruz, M D B Arrigoni, A L N Rigueiro, J Silva, T V B Carrara, P C S Santos, L L Cursino, D D Millen
The objective of this study was to examine the relationship of DMI fluctuation, feedlot performance, feeding behavior, rumen morphometrics, and carcass characteristics in Nellore cattle classified by residual feed intake (RFI). One experiment was conducted in 2 consecutive years using individual pens (1.0 × 7.0 m) at the São Paulo State University feedlot, Dracena campus, Brazil. The experiment in year 1 started in June of 2012 with forty-eight 20-mo-old Nellore yearling bulls with an initial BW of 358.2 ± 19...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27896777/-1-h-nmr-metabolomic-profiling-of-human-and-animal-blood-serum-samples
#13
João G M Pontes, Antonio J M Brasil, Guilherme C F Cruz, Rafael N de Souza, Ljubica Tasic
Nuclear magnetic resonance (NMR) spectroscopy techniques allow the acquisition of a large amount of data and when combined with multivariate statistical analysis, it is possible to process and interpret the obtained NMR data in accordance with the biological problem being investigated. In this chapter, the search for biologically relevant biomarkers is addressed using NMR spectroscopy-based metabolomics, due to their clinical relevance for either diagnosis or monitoring of diseases and disorders.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27896641/seom-clinical-guideline-of-fertility-preservation-and-reproduction-in-cancer-patients-2016
#14
M Muñoz, A Santaballa, M A Seguí, C Beato, S de la Cruz, J Espinosa, P J Fonseca, J Perez, T Quintanar, A Blasco
Chemotherapy and radiotherapy often result in reduced fertility in cancer patients. With increasing survival rates, fertility is an important quality-of-life concern for many young cancer patients. Around 70-75% of young cancer survivors are interested in parenthood but the numbers of patients who access fertility preservation techniques prior to treatment are significantly lower. Moreover, despite existing guidelines, healthcare professionals do not address fertility preservation issues adequately. There is a critical need for improvements in clinical care to ensure patients are well informed about infertility risks and fertility preservation options and to support them in their reproductive decision-making prior to cancer treatment...
November 28, 2016: Clinical & Translational Oncology
https://www.readbyqxmd.com/read/27893750/molecular-epidemiology-of-agents-of-human-chromoblastomycosis-in-brazil-with-the-description-of-two-novel-species
#15
Renata R Gomes, Vania A Vicente, Conceição M P S de Azevedo, Claudio G Salgado, Moises B da Silva, Flávio Queiroz-Telles, Sirlei G Marques, Daniel W C L Santos, Tania S de Andrade, Elizabeth H Takagi, Katia S Cruz, Gheniffer Fornari, Rosane C Hahn, Maria L Scroferneker, Rachel B Caligine, Mauricio Ramirez-Castrillon, Daniella P de Araújo, Daiane Heidrich, Arnaldo L Colombo, G S de Hoog
The human mutilating disease chromoblastomycosis is caused by melanized members of the order Chaetothyriales. To assess population diversity among 123 clinical strains of agents of the disease in Brazil we applied sequencing of the rDNA internal transcribed spacer region, and partial cell division cycle and β-tubulin genes. Strains studied were limited to three clusters divided over the single family Herpotrichiellaceae known to comprise agents of the disease. A Fonsecaea cluster contained the most important agents, among which F...
November 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27893347/asymptomatic-infections-in-blood-donors-harbouring-plasmodium-an-invisible-risk-detected-by-molecular-and-serological-tools
#16
Giselle F M C Lima, Maria C Arroyo Sanchez, José E Levi, Mahyumi Fujimori, Luiza Da Cruz Caramelo, Arianni Rondelli Sanchez, Eduardo M Ramos-Sanchez, Juliana Inoue, Maria De Jesus Costa-Nascimento, Alfredo Mendrone Junior, Silvia M Di Santi
BACKGROUND: Transfusion-transmitted malaria due to asymptomatic Plasmodium infections is a challenge for blood banks. There is a lack of data on the prevalence of asymptomatic infected blood donors and the incidence of transfusion-transmitted malaria in low endemicity areas worldwide. We estimated the frequency of blood donors harbouring Plasmodium in an area in which asymptomatic infections have been reported. MATERIAL AND METHODS: To estimate the frequency of blood donors harbouring Plasmodium we used microscopy and molecular tools...
November 7, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27893193/the-oil-contaminated-soil-diazotroph-azoarcus-olearius-dqs-4-t-is-genetically-and-phenotypically-similar-to-the-model-grass-endophyte-azoarcus-sp-bh72
#17
Helisson Faoro, Rodrigo Rene Menegazzo, Federico Battistoni, Prasad Gyaneshwar, Fernanda P do Amaral, Cecilia Taulé, Sydnee Rausch, Patricia Gonçalves Galvão, Cecilia de Los Santos, Shubhajit Mitra, Gabriela Heijo, Shih-Yi Sheu, Wen-Ming Chen, Cintia Mareque, Michelle Zibetti Tadra-Sfeir, J Ivo Baldani, Marta Maluk, Ana Paula Guimarães, Gary Stacey, Emanuel M de Souza, Fabio O Pedrosa, Leonardo Magalhães Cruz, Euan K James
The genome of Azoarcus olearius DQS-4(T) , a N2 -fixing Betaproteobacterium isolated from oil-contaminated soil in Taiwan, was sequenced and compared with other Azoarcus strains. The genome sequence showed high synteny with Azoarcus sp. BH72, a model endophytic diazotroph, but low synteny with five non-plant-associated strains (Azoarcus CIB, Azoarcus EBN1, Azoarcus KH32C, A. toluclasticus MF63(T) and Azoarcus PA01). Average Nucleotide Identity (ANI) revealed that DQS-4(T) shares 98.98% identity with Azoarcus BH72, which should now be included in the species A...
November 28, 2016: Environmental Microbiology Reports
https://www.readbyqxmd.com/read/27893135/screening-for-colorectal-cancer-and-evolving-issues-for-physicians-and-patients-a-review
#18
REVIEW
David Lieberman, Uri Ladabaum, Marcia Cruz-Correa, Carla Ginsburg, John M Inadomi, Lawrence S Kim, Francis M Giardiello, Richard C Wender
Importance: Colorectal cancer (CRC) is the second-leading cause of cancer death in the United States. Screening can reduce CRC mortality and incidence, and numerous screening options, although available, complicate informed decision making. This review provides evidence-based tools for primary care physicians to identify patients with higher-than-average-risk and engage patients in informed decision making about CRC screening options. Observations: Recently, the US Preventive Services Task Force recommended any of 8 CRC screening approaches for average-risk individuals, beginning at age 50 years...
November 22, 2016: JAMA: the Journal of the American Medical Association
https://www.readbyqxmd.com/read/27891505/comparative-genomics-of-the-conjugation-region-of-f-like-plasmids-five-shades-of-f
#19
Raul Fernandez-Lopez, Maria de Toro, Gabriel Moncalian, M Pilar Garcillan-Barcia, Fernando de la Cruz
The F plasmid is the foremost representative of a large group of conjugative plasmids, prevalent in Escherichia coli, and widely distributed among the Enterobacteriaceae. These plasmids are of clinical relevance, given their frequent association with virulence determinants, colicins, and antibiotic resistance genes. Originally defined by their sensitivity to certain male-specific phages, IncF plasmids share a conserved conjugative system and regulatory circuits. In order to determine whether the genetic architecture and regulation circuits are preserved among these plasmids, we analyzed the natural diversity of F-like plasmids...
2016: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/27886330/genetic-gains-from-selection-for-fiber-traits-in-gossypium-hirsutum-l
#20
G M P de Faria, C F B Sanchez, L P de Carvalho, M da Silva Oliveira, C D Cruz
Brazil is among the five largest producers of cotton in the world, cultivating the species Gossypium hirsutum L. r. latifolium Hutch. The cultivars should have good fiber quality as well as yield. Genetic improvement of fiber traits requires the study of the genetic structure of the populations under improvement, leading to the identification of promising parent plants. To this end, it is important to acquire some information, such as estimates of genetic variance components and heritability coefficients, which will support the appropriate choice of the breeding strategy to be employed as well as enable the estimation of gains from selection...
November 21, 2016: Genetics and Molecular Research: GMR
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