Seung-Won Choi, Kie Kumaishi, Reiko Motohashi, Harumi Enoki, Wiluk Chacuttayapong, Tadashi Takamizo, Hiroaki Saika, Masaki Endo, Tetsuya Yamada, Aya Hirose, Nobuya Koizuka, Seisuke Kimura, Yaichi Kawakatsu, Hiroyuki Koga, Emi Ito, Ken Shirasu, Yasunori Ichihashi
Agrobacterium -mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered that oxicam-type nonsteroidal anti-inflammatory drugs, including tenoxicam (TNX), increase the efficiency of Agrobacterium -mediated transient transformation. TNX treatment increased the transformation efficiency of Agrobacterium -mediated transformation of Arabidopsis thaliana mature leaves by agroinfiltration...
September 25, 2022: Plant Biotechnology (Tokyo, Japan)