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Tatiana da Silva Fonseca de Oliveira, Barbara Neves Dos Santos, Tainah Silva Galdino, Alejandro Marcel Hasslocher-Moreno, Otilio Machado Pereira Bastos, Maria Auxiliadora de Sousa
INTRODUCTION: Trypanosoma cruzi is the etiologic agent of Chagas disease in humans, mainly in Latin America. Trypanosome stocks were isolated by hemoculture from patients followed at Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ) and studied using different approaches. METHODS: For species and genotype identification, the stocks were analyzed by parasitological techniques, polymerase chain reaction assays targeted to specific DNA sequences, isoenzyme patterns, besides sequencing of a polymorphic locus of TcSC5D gene (one stock)...
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
Priscilla Almeida da Costa, Marcela Segatto, Danielle Fernandes Durso, Wagson José de Carvalho Moreira, Lucas Lodi Junqueira, Fábio Morato de Castilho, Silvio Amadeu de Andrade, Cláudio Léo Gelape, Egler Chiari, Andréa Teixeira-Carvalho, Sergio Danilo Junho Pena, Carlos Renato Machado, Gloria Regina Franco, Geraldo Brasileiro Filho, Maria da Consolação Vieira Moreira, Andréa Mara Macedo
BACKGROUND: Heart transplantation is a valuable therapeutic option for Chagas disease patients with severe cardiomyopathy. During patient follow-up, the differential diagnosis between cardiac transplant rejection and Chagas disease infection reactivation remains a challenging task, which hinders rapid implementation of the appropriate treatment. Herein we investigate whether polymerase chain reaction (PCR) strategies could facilitate early detection of Trypanosoma cruzi (T cruzi) in transplanted endomyocardial biopsies (EMBs)...
February 24, 2017: Journal of Heart and Lung Transplantation
David A Leiby, Megan L Nguyen, Melanie C Proctor, Rebecca L Townsend, Susan L Stramer
BACKGROUND: Trypanosoma cruzi is endemic to the Americas where it demonstrates multiple lineages over a vast geographic range (i.e., United States to Argentina). These lineages possess divergent geographic and biologic characteristics, including variations in disease manifestations. Herein, we report the frequency of parasitemia among seropositive US blood donors and the potential association between parasite lineage and transfusion transmission. STUDY DESIGN AND METHODS: Blood donors identified as T...
March 14, 2017: Transfusion
Lenka Micenková, Alžbeta Beňová, Lucia Frankovičová, Juraj Bosák, Martin Vrba, Alena Ševčíková, Marta Kmeťová, David Šmajs
Escherichia coli is the most common cause of bloodstream infections and community-acquired sepsis. The main aim of this study was to determine virulence characteristics of E. coli isolates from hemocultures of patients with a primary disease of urogenital tract, digestive system, a neoplastic blood disease, or other conditions. Results from a set of 314 E. coli isolates from hemocultures were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. Genetic profiling of the 314 E...
April 2017: International Journal of Medical Microbiology: IJMM
Murat Sütçü, Hacer Aktürk, Fatih Karagözlü, Ayper Somer, Nezahat Gürler, Nuran Salman
BACKGROUND: Clinical vaccine failure is the occurence of the specific vaccine-preventable disease in an appropriately and fully vaccinated person after enough time has elapsed for protection against the antigens of the vaccine to develop. Fully immunized cases with pneumoccal vaccine may sometimes develop a complicated pneumonia with empyema caused by a vaccine serotype. CASE REPORT: A 2 year-old male patient was admitted with the complaints of fever. On the basis of findings and laboratory results, the patient was diagnosed as having empyema...
January 2017: Balkan Medical Journal
Pear Pongsachareonnont, Worawalun Honglertnapakul, Tanittha Chatsuwan
BACKGROUND: Identification of bacterial pathogens in endophthalmitis is important to inform antibiotic selection and treatment decisions. Hemoculture bottles and polymerase chain reaction (PCR) analysis have been proposed to offer good detection sensitivity. This study compared the sensitivity and accuracy of a blood culture system, a PCR approach, and conventional culture methods for identification of causative bacteria in cases of acute endophthalmitis. METHODS: Twenty-nine patients with a diagnosis of presumed acute bacterial endophthalmitis who underwent vitreous specimen collection at King Chulalongkorn Memorial Hospital were enrolled in this study...
February 21, 2017: BMC Infectious Diseases
Hatice Kaya, Yüksel Turan, Sade Akbal, Keziban Tosun, Esra Aksoy, Yusuf Tunalı, Gülsün Özdemir Aydın
PURPOSE: This clinical study was planned to determine the effect of Nursing Care Protocol on the prevention of central venous catheter-related infections in neurosurgery intensive care unit. METHOD: The study was conducted between January 2012 and December 2013. The sampling consisted of 160 patients compatible with study inclusion criteria (80 protocol and 80 control patients). Routine care was applied to the control group while the protocol group received catheter care according to the protocol formed in accordance with the suggestions of the Centers for Disease Control and Prevention...
November 2016: Applied Nursing Research: ANR
Z Canan Özdemir, Ahmet Koç, Ali Ayçiçek
Chemotherapy induced febrile neutropenia predisposes patients to life threatening infections. We aimed to determine the causative microorganisms, infection focus and antibiotic treatment success in febrile neutropenic children with leukemia. A total of 136 febrile neutropenic episodes in 48 leukemic children were reviewed retrospectively from records. Among 136 febrile neutropenic episodes, 68 (50%) episodes were microbiologically documented. Methicillin sensitive coagulase (-) Staphylococcus aureus were the most common isolates from hemoculture (20...
2016: Turkish Journal of Pediatrics
Ana Paula Margioto Teston, Ana Paula de Abreu, Camila Piva Abegg, Mônica Lúcia Gomes, Max Jean de Ornelas Toledo
A new epidemiological view of American trypanosomiasis or Chagas disease has been formulated in recent decades. Oral transmission of the etiological agent of Chagas disease, Trypanosoma cruzi, has been the most common form of transmission. The T. cruzi discrete typing units TcI and TcIV have been involved in tens outbreaks of acute cases of Chagas disease in the Brazilian Amazon region. We investigated the intensity of infection in mice that were orally inoculated (OR group) with four strains of TcIV that were isolated from two outbreaks of acute Chagas disease that was orally acquired in the state of Amazonas, Brazil...
February 2017: Acta Tropica
Maria Augusta Dario, Marina Silva Rodrigues, Juliana Helena da Silva Barros, Samanta Cristina das Chagas Xavier, Paulo Sérgio D'Andrea, André Luiz Rodrigues Roque, Ana Maria Jansen
BACKGROUND: Trypanosoma cruzi infection via oral route results in outbreaks or cases of acute Chagas disease (ACD) in different Brazilian regions and poses a novel epidemiological scenario. In the Espírito Santo state (southeastern Brazil), a fatal case of a patient with ACD led us to investigate the enzootic scenario to avoid the development of new cases. At the studied locality, Triatoma vitticeps exhibited high T. cruzi infection rates and frequently invaded residences. METHODS: Sylvatic and domestic mammals in the Rio da Prata locality, where the ACD case occurred, and in four surrounding areas (Baia Nova, Buenos Aires, Santa Rita and Todos os Santos) were examined and underwent parasitological and serological tests...
2016: Parasites & Vectors
Etsuko Takizawa, Koichi Yamada, Ken-Ichi Oinuma, Kanako Sato, Mamiko Niki, Hiroki Namikawa, Hiroki Fujimoto, Kazuhisa Asai, Yukihiro Kaneko, Hiroshi Kakeya
We herein report the first domestic case of bacteremia caused by an intrinsic strain of colistin-resistant Acinetobacter. The Acinetobacter species was detected in the hemocultures in a febrile patient. The patient was a 65-year-old-man who was admitted to our hospital for laparotomic gastrostomy. The patient's antimicrobial susceptibility patterns were atypical; they were colistin resistant but not multiple drug resistant. A sequence analysis of rpoB identified the bacterium as an Acinetobacter genomic species 13BJ/14TU, which had only been previously reported in South Korea...
2016: Internal Medicine
Frédéric Baroz, Priscile Clément, Monica Levy, Hervé Duplain
This article reports one of the rare cases of Abiotrophia defectiva endocarditis with no underlying valvular condition. A sixty-three years old man was hospitalized because of complicated respiratory sepsis with acute heart failure. Hemocultures and echocardiogram enabled the diagnosis of A. defectiva endocarditis. The clinical course was favorable under combined aminoglycoside and cephalosporin. The patient ultimately required valvular replacement. A. defectiva is a micro-organism part of the Nutritionnaly Variant Streptococci (NVS) associated with a high mortality rate and often resistant to antibiotics...
June 22, 2016: Revue Médicale Suisse
Panita Worapratya, Jutharat Joraluck, Apisit Wanjaroenchaisuk, Prasit Wuthisuthimethawee
OBJECTIVE: Determine the appropriateness of broad-spectrum antibiotics applied in severe sepsis and septic shock in an emergency room and its impact on the survival rate. MATERIAL AND METHOD: This was a prospective observational study in an emergency room setting at a tertiary care facility where early goal-directed therapy (EGDT) was applied for resuscitation of severe septic and septic shock patients. The data recorded were the initial vital signs, SAP II score, time of EGDT goals achieved (ScvO₂> 70), time of antibiotics initiated, type of antibiotics used, organisms that were identified, source of infection, initial and final diagnosis, and outcome of treatment...
May 2016: Journal of the Medical Association of Thailand, Chotmaihet Thangphaet
Sabine Dittrich, Elizabeth Card, Weerawat Phuklia, Williams E Rudgard, Joy Silousok, Phonelavanh Phoumin, Latsaniphone Bouthasavong, Sarah Azarian, Viengmon Davong, David A B Dance, Manivanh Vongsouvath, Rattanaphone Phetsouvanh, Paul N Newton
Orientia tsutsugamushi, which requires specialized facilities for culture, is a substantial cause of disease in Asia. We demonstrate that O. tsutsugamushi numbers increased for up to 5 days in conventional hemocultures. Performing such a culture step before molecular testing could increase the sensitivity of O. tsutsugamushi molecular diagnosis.
August 2016: Emerging Infectious Diseases
Fernanda Moreira Alves, Juliane Saab de Lima, Fabiana Lopes Rocha, Heitor Miraglia Herrera, Guilherme de Miranda Mourão, Ana Maria Jansen
BACKGROUND: Trypanosoma cruzi is dispersed in nature through many transmission mechanisms among a high diversity of vectors and mammalian species, representing particular behaviors and habitats. Thus, each locality has a unique set of conditions underlying the maintenance of this parasite in the wild. The aim of the present study was to evaluate the life-cycle of T. cruzi in free-ranging coatis from the central region of the Brazilian Pantanal using a multi-factorial approach. METHODS: Three methodological blocks were used in the present study: (i) We evaluated T...
July 1, 2016: Parasites & Vectors
Carlos Geraldo Campos de Mello, Renata Tupinambá Branquinho, Maykon Tavares Oliveira, Matheus Marques Milagre, Dênia Antunes Saúde-Guimarães, Vanessa Carla Furtado Mosqueira, Marta de Lana
The etiological treatment of Chagas disease remains neglected. The compounds available show several limitations, mainly during the chronic phase. Lychnopholide encapsulated in polymeric nanocapsules (LYC-NC) was efficacious in mice infected with Trypanosoma cruzi and treated by intravenous administration during the acute phase (AP). As the oral route is preferred for treatment of chronic infections, such as Chagas disease, this study evaluated the use of oral LYC-NC in the AP and also compared it with LYC-NC administered to mice by the oral and intravenous routes during the chronic phase (CP)...
September 2016: Antimicrobial Agents and Chemotherapy
B Vincenzi, I Fioroni, F Pantano, S Angeletti, G Dicuonzo, A Zoccoli, D Santini, G Tonini
In oncologic patients fever is a non-specific clinical marker of different clinical settings. Procalcitonin (PCT) seems to be the most promising infection marker. We aimed to define the potential role of PCT as an earlier diagnostic marker in patients with fever and solid tumor. This retrospective study enrolled 431 patients. All of them performed hemoculture (HE) and basal PCT assessment (reference laboratory cut-off: ≤0.5 or >0.5 ng/dL) before starting antibiotic therapy. Gram positive (G+), negative (G-) or Fungi infection were detected...
2016: Scientific Reports
Miroslav Kojić, Darko Nozić, Olivera Tarabar, Nenad Perisić
INTRODUCTION: Extraintestinal manifestations of nontyphoidal salmonellosis are usually seen in patients with cellular immunodeficiency. Pleural empyema caused by nontyphoidal Salmonella is very rare clinical presentation of salmonellosis and there are just a few cases described in a literature. We presented a very rare case of pleural empyema caused by Salmonella enteritidis in a patient with non-Hodgkin limphoma. CASE REPORT: A 60-year-old male with low grade B-cell lymphoma, mucosa associated lymphoid tissue (MALT) type in IV clinical degree, manifested with infiltration of stomach, bronchus, pleura and peritoneum was admitted to the hospital...
March 2016: Vojnosanitetski Pregled. Military-medical and Pharmaceutical Review
F Crokaert, M Blogie, T Prigoginc, E Yourassowsky
114 strains isolated by blood cultures and transtracheal aspirations were typed: serotypes 3 and 4 were largely predominant and a 14-valent vaccine (1, 2, 3, 4, 6, 8, 9, 12, 14, 19, 23, 25, 51, 56) should cover 67 % of these strains and protect 53 % of our patients because the efficacy of the vaccine is evaluated at 80 %. A controlled clinical trial seems to be necessary. We did not isolate any penicillin-resistant strain but 14 % of our strains were resistant to tetracycline.
January 1980: Acta Clinica Belgica
E S Lisitsina, T V Chernenkaya, E N Ilyina, I V Lazareva, V A Ageevets, S V Sidorenko
Fifteen specimens of the hemoculture and 89 specimens of the broncho-alveolar lavage were used in the study. Monocultures of gramnegative bacteria resistant to cefotaxime, cefepime, imipenem and meropenem were isolated from the specimens. The PCR method with detection of the results in the real time regimen (PCR test-system Litekh) provided detection of the beta-lactamase genes: bla(CTX-M-like) (72/104, 69.2%), bla(NDM) (6/104, 5.8%), bla(VIM) (49/104, 47.1%) and bla(OXA48-like) (59/104, 56.7%). There was identified correlation between the phenotype of resistance of Acinetobacter spp...
2015: Antibiotiki i Khimioterapii︠a︡, Antibiotics and Chemoterapy [sic]
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