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https://www.readbyqxmd.com/read/28101157/functional-analysis-of-keratinocyte-and-fibroblast-gene-expression-in-skin-and-keloid-scar-tissue-based-on-deviation-analysis-of-dynamic-capabilities
#1
Mingming Li, Lei Wu
The aim of the present study was to select key genes that are associated with fibroblasts and keratinocytes during keloid scar progression and development. The gene expression profile of GSE44270, which includes 32 samples, was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) in case samples compared with control samples were screened using the Limma R package followed by hierarchical clustering analysis. Protein-protein interaction (PPI) networks of the total selected DEGs were constructed using Cytoscape...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28099503/3d-functional-corneal-stromal-tissue-equivalent-based-on-corneal-stromal-stem-cells-and-multi-layered-silk-film-architecture
#2
Chiara E Ghezzi, Benedetto Marelli, Fiorenzo G Omenetto, James L Funderburgh, David L Kaplan
The worldwide need for human cornea equivalents continues to grow. Few clinical options are limited to allogenic and synthetic material replacements. We hypothesized that tissue engineered human cornea systems based on mechanically robust, patterned, porous, thin, optically clear silk protein films, in combination with human corneal stromal stem cells (hCSSCs), would generate 3D functional corneal stroma tissue equivalents, in comparison to previously developed 2D approaches. Silk film contact guidance was used to control the alignment and distribution of hCSSCs on RGD-treated single porous silk films, which were then stacked in an orthogonally, multi-layered architecture and cultured for 9 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28060071/corneal-lenticule-allotransplantation-after-femtosecond-laser-small-incision-lenticule-extraction-in-rabbits
#3
Jing Zhao, Yang Shen, Mi Tian, Ling Sun, Yu Zhao, Xiaoyu Zhang, Xingtao Zhou
PURPOSE: To investigate the feasibility of allotransplanting extracted lenticules after femtosecond laser-assisted small incision lenticule extraction (SMILE) in rabbits and the subsequent healing process. METHODS: Fourteen New Zealand white rabbits were divided evenly into 2 groups. The rabbits in group A received SMILE procedures with a -6.00 D correction. The lenticules from group A were immediately inserted into a femtosecond laser-created corneal stromal pocket in group B...
February 2017: Cornea
https://www.readbyqxmd.com/read/28026154/surface-topography-and-mechanical-strain-promote-keratocyte-phenotype-and-extracellular-matrix-formation-in-a-biomimetic-3d-corneal-model
#4
Wei Zhang, Jialin Chen, Ludvig J Backman, Adam D Malm, Patrik Danielson
The optimal functionality of the native corneal stroma is mainly dependent on the well-ordered arrangement of extracellular matrix (ECM) and the pressurized structure. In order to develop an in vitro corneal model, it is crucial to mimic the in vivo microenvironment of the cornea. In this study, the influence of surface topography and mechanical strain on keratocyte phenotype and ECM formation within a biomimetic 3D corneal model is studied. By modifying the surface topography of materials, it is found that patterned silk fibroin film with 600 grooves mm(-1) optimally supports cell alignment and ECM arrangement...
December 27, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28018671/accelerated-corneal-collagen-cross-linking-using-topography-guided-uv-a-energy-emission-preliminary-clinical-and-morphological-outcomes
#5
Cosimo Mazzotta, Antonio Moramarco, Claudio Traversi, Stefano Baiocchi, Alfonso Iovieno, Luigi Fontana
Purpose. To assess the clinical and morphological outcomes of topography-guided accelerated corneal cross-linking. Design. Retrospective case series. Methods. 21 eyes of 20 patients with progressive keratoconus were enrolled. All patients underwent accelerated cross-linking using an ultraviolet-A (UVA) exposure with an energy release varying from 7.2 J/cm(2) up to 15 J/cm(2), according to the topographic corneal curvature. Uncorrected (UDVA) and corrected (CDVA) distance visual acuity, topography, in vivo confocal microscopy (IVCM), and anterior segment optic coherence tomography (AS-OCT) were evaluated preoperatively and at the 1, 3, 6, and 12 months postoperatively...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27987771/adhesion-and-metabolic-activity-of-human-corneal-cells-on-pcl-based-nanofiber-matrices
#6
Piotr Stafiej, Florian Küng, Daniel Thieme, Marta Czugala, Friedrich E Kruse, Dirk W Schubert, Thomas A Fuchsluger
In this work, polycaprolactone (PCL) was used as a basic polymer for electrospinning of random and aligned nanofiber matrices. Our aim was to develop a biocompatible substrate for ophthalmological application to improve wound closure in defects of the cornea as replacement for human amniotic membrane. We investigated whether blending the hydrophobic PCL with poly (glycerol sebacate) (PGS) or chitosan (CHI) improves the biocompatibility of the matrices for cell expansion. Human corneal epithelial cells (HCEp) and human corneal keratocytes (HCK) were used for in vitro biocompatibility studies...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27939309/adhesion-dependent-wave-generation-in-crawling-cells
#7
Erin L Barnhart, Jun Allard, Sunny S Lou, Julie A Theriot, Alex Mogilner
Dynamic actin networks are excitable. In migrating cells, feedback loops can amplify stochastic fluctuations in actin dynamics, often resulting in traveling waves of protrusion. The precise contributions of various molecular and mechanical interactions to wave generation have been difficult to disentangle, in part due to complex cellular morphodynamics. Here we used a relatively simple cell type-the fish epithelial keratocyte-to define a set of mechanochemical feedback loops underlying actin network excitability and wave generation...
January 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/27930797/unusual-severe-interface-inflammation-after-uneventful-small-incision-lenticule-extraction-smile
#8
Damien Guindolet, Abdellatif Badaoui, Maud Elluard, Sophie Stephan, Serge Doan, Isabelle Cochereau, Eric E Gabison
PURPOSE: To describe a 40-year-old woman who complained of visual loss in her left eye 3 days after an uneventful bilateral myopic refractive surgery by small incision lenticule extraction (SMILE). METHODS: Case report. RESULTS: A 4 × 5 mm anterior central stromal infiltration was observed with moderate pain associated with decreased corneal sensitivity and neither discharge nor fluorescein staining. Bacterial and fungal smears and cultures from the SMILE interface were negative and local antibiotic ineffective...
December 1, 2016: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/27924359/effects-of-4-methylumbelliferone-and-high-molecular-weight-hyaluronic-acid-on-the-inflammation-of-corneal-stromal-cells-induced-by-lps
#9
Fang Li, Peng Hao, Guangjie Liu, Weiyi Wang, Ruifang Han, Zhixin Jiang, Xuan Li
PURPOSE: To investigate the effects of hyaluronic acid (HA) on the inflammation of corneal fibroblasts induced by lipopolysaccharide (LPS). METHODS: Primary rabbit corneal keratocytes were isolated with collagenase. The keratocytes were cultured in a serum-containing medium to induce corneal fibroblasts, which represented the wound repair phenotype of corneal keratocytes. Corneal fibroblasts were treated with LPS with or without 4-methylumbelliferone (4-MU) / high molecular weight hyaluronic acid (HMWHA)...
December 6, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27818315/il-20-promotes-epithelial-healing-of-the-injured-mouse-cornea
#10
Wanyu Zhang, Sri Magadi, Zhijie Li, C Wayne Smith, Alan R Burns
After corneal epithelial injury, the ensuing inflammatory response is necessary for efficient wound healing. While beneficial healing effects are attributed to recruited neutrophils and platelets, dysregulated inflammation (too little or too much) is associated with impaired wound healing. The purpose of this study was to use an established C57BL/6J mouse model of corneal injury to evaluate the potential modulatory role of interleukin-20 (IL-20) on the inflammatory and healing responses to epithelial wounding...
November 3, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27815483/effect-of-autologous-serum-eye-drops-in-patients-with-sj%C3%A3-gren-syndrome-related-dry-eye-clinical-and-in-vivo-confocal-microscopy-evaluation-of-the-ocular-surface
#11
Francesco Semeraro, Eliana Forbice, Giuseppe Nascimbeni, Marco Taglietti, Vito Romano, Germano Guerra, Ciro Costagliola
AIM: To evaluate in vivo changes after therapy using autologous serum (AS) eye drops in Sjögren's syndrome (SS)-related dry eyes by confocal microscopy. PATIENTS AND METHODS: In this study, 24 patients with SS-related dry eyes [12 in AS eye drop therapy and 12 in artificial tear (AT) therapy] and 24 healthy volunteers were recruited. Ocular Surface Disease Index (OSDI), central corneal thickness, tear film, break-up time, corneal and conjunctival staining, Schirmer's test and corneal confocal microscopy were investigated...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27802519/antiapoptotic-effect-of-acetylcholine-in-fas-induced-apoptosis-in-human-keratocytes
#12
Marta Sloniecka, Ludvig J Backman, Patrik Danielson
Purpose: To investigate the possible antiapoptotic effect of acetylcholine (ACh) in Fas-mediated apoptosis of primary human keratocytes in vitro, and to explore the underlying mechanism. Methods: Primary human keratocytes were isolated from healthy corneas. Fas ligand (FasL) was used to induce apoptosis in keratocytes. Cell death was assessed by ELISA. Activity of caspase-3, -7, -8, and -9 was measured with luminescent caspase activity assays. Expression of nuclear factor-κB (NF-κB) gene was assessed with RT-quantitative (q)PCR...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27792791/derivation-of-corneal-keratocyte-like-cells-from-human-induced-pluripotent-stem-cells
#13
Richard W Naylor, Charles N J McGhee, Chad A Cowan, Alan J Davidson, Teresa M Holm, Trevor Sherwin
Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based therapies may offer an alternative approach given that the eye is amenable to such treatments and corneal diseases like keratoconus have been associated specifically with the death of corneal keratocytes. The ability to generate corneal keratocytes in vitro may enable a cell-based therapy to treat patients with keratoconus...
2016: PloS One
https://www.readbyqxmd.com/read/27783156/arginase-activity-urea-and-hydroxyproline-concentration-are-reduced-in-keratoconus-keratocytes
#14
Tanja Stachon, Krasimir Kolev, Zsuzsa Flaskó, Berthold Seitz, Achim Langenbucher, Nóra Szentmáry
PURPOSE: Keratoconus (KC) is a disease characterized by thinning and deformation of the cornea, but its etiology remains unknown. Seventy percent of the corneal stroma consists of collagen, which is composed of three intertwined polypeptide chains with glycine-hydroxyproline-proline repeats along their sequence. Arginase is a cytoplasmatic enzyme and catalyzes the conversion of arginine to urea and ornithine, which serves as a precursor for the endogenous synthesis of proline and hydroxyproline...
January 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27781955/mmp14-regulates-vegfr3-expression-on-corneal-epithelial-cells
#15
Kyu-Yeon Han, Jin-Hong Chang, Dimitri T Azar
Vascular endothelial growth factor receptor 3 (VEGFR3) regulates the growth and differentiation of blood and lymphatic vessels. To determine whether matrix metalloproteinase 14 (MMP14) modulates VEGFR3 expression in the corneal epithelium to influence the avascularity of the cornea, VEGFR3 expression was compared between wild-type and MMP14-deficient (MMP14 Δexon4) corneal epithelial cells. Western blot analysis showed that VEGFR3 protein expression was higher on MMP14 Δexon4 corneal epithelial cells than on wild-type cells, and quantitative RT-PCR analysis showed that VEGFR3 gene expression was highly induced in MMP14 Δexon4 corneal epithelial cells but not in wild-type corneal epithelial cells or wild-type and MMP14 Δexon4 corneal keratocytes...
2016: Protein and Peptide Letters
https://www.readbyqxmd.com/read/27770837/effect-of-riboflavin-uva-collagen-cross-linking-on-central-cornea-limbus-and-intraocular-pressure-experimental-study-in-rabbit-eyes
#16
Vassilios Kozobolis, Maria Gkika, Haris Sideroudi, Efthymia Tsaragli, Stylliani Lydataki, Irini Naoumidi, Alexandra Giatromanolaki, Dimitrios Mikropoulos, Miguel Teus, Georgios Labiris
The Purpose of present study was to investigate the effect of riboflavin/ultraviolet-A-induced collagen cross-linking (CXL) on central cornea, limbus and intraocular pressure (IOP). This was an animal experimental study. The right corneas of 10 rabbits were ultraviolet-A irradiated (3 mW/cm2 for 30 minutes) after de-epithelialization and instillation of 0.1% riboflavin / 20% Dextran drops. Left corneas served as controls. Samples were examined histologically one month postoperatively. Before and after treatment, IOP measurements were recorded bilaterally...
2016: Acta Medica (Hradec Králové)
https://www.readbyqxmd.com/read/27760648/-study-on-the-evaluation-of-pterygium-activity-with-in-vivo-confocal-microscopy
#17
Q F Liang, C Gao, H Liang, B Li, X H Du, Antoine Labbe
Objective: To observe the characteristics of pterygium with in vivo confocal microscopy (IVCM) and understand pterygium activity with the density of inflammatory cells, formation of new blood vessels, and the number of activated keratocytes within the stroma. Methods: A prospective case-controlled study. Thirty-six pterygia from 28 patients were analyzed in this study. A pterygium activity score was obtained by summing four scores of ocular discomfort, pterygium hyperemia, keratitis, and the presence of Fuchs patches...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27749452/comparative-confocal-and-histopathological-study-of-corneal-changes-in-multiple-myeloma
#18
Antonio Micali, Anna M Roszkowska, Elisa I Postorino, Laura Rania, Emanuela Aragona, Edward Wylegala, Anna Nowinska, Antonio Ieni, Sebastiano Calimeri, Antonina Pisani, Pasquale Aragona, Domenico Puzzolo
PURPOSE: Corneal opacities rarely occur in multiple myeloma (MM). Our study correlates the findings of in vivo confocal microscopy (IVCM), a useful diagnostic tool, with histopathological features of corneal opacities appearing in a patient with MM. METHODS: Case report. RESULTS: A 53-year-old man developed corneal opacities in both eyes, more pronounced in the left eye. After IVCM examination, he underwent penetrating keratoplasty in the left eye, and the button was processed for light and electron microscopy and immunohistochemistry...
January 2017: Cornea
https://www.readbyqxmd.com/read/27732879/temporal-and-spatial-analysis-of-stromal-cell-and-extracellular-matrix-patterning-following-lamellar-keratectomy
#19
Pouriska B Kivanany, Kyle C Grose, W Matthew Petroll
Extracellular matrix (ECM) supplies both physical and chemical signals to keratocytes which can impact their differentiation to fibroblasts and/or myofibroblasts. It also provides a substrate through which they migrate during wound repair. We have previously shown that following transcorneal freeze injury (FI), migrating corneal fibroblasts align parallel to the stromal lamellae during wound repopulation. In this study, we compare cell and ECM patterning both within and on top of the stroma at different time points following lamellar keratectomy (LK) in the rabbit...
December 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27727144/effect-of-substrate-elasticity-on-macroscopic-parameters-of-fish-keratocyte-migration
#20
Daniel Dziob, Tomasz Kołodziej, Justyna Nowak, Piotr Cyzio, Joanna Raczkowska, Jadwiga Laska, Zenon Rajfur
Cell migration is an important biological phenomenon which depends on a number of internal and external factors. One of such factors can be the mechanical properties of the environment which can have an impact on the cell's regulatory pathways through so-called mechanotransduction. Ultimately, these properties can also influence the process of cell migration. The goal of this work is to investigate how substrate stiffness (elasticity) changes basic migration parameters of migrating cells. Fish keratocytes migrating on polyacrylamide hydrogels have been used as a model of fast migrating cells...
October 11, 2016: Physical Biology
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