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https://www.readbyqxmd.com/read/28532937/collagen-crosslinking-with-conventional-and-accelerated-ultraviolet-a-irradiation-using-riboflavin-with-hydroxypropyl-methylcellulose
#1
Anne Marie Hagem, Andreas Thorsrud, Gunhild Falleth Sandvik, Marianne Råen, Liv Drolsum
PURPOSE: To evaluate corneal collagen crosslinking (CXL) with conventional and accelerated ultraviolet-A (UVA) irradiation using riboflavin with methylcellulose. SETTING: Department of Ophthalmology, Oslo University Hospital, Oslo, Norway. DESIGN: Prospective randomized case series. METHODS: Patients with keratoconus were randomized to have CXL using conventional 3 mW/cm(2) UVA irradiation for 30 minutes (CXL30) or accelerated 9 mW/cm(2) UVA irradiation for 10 minutes (CXL10)...
April 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28503733/long-term-safety-from-transmission-of-porcine-endogenous-retrovirus-after-pig-to-non-human-primate-corneal-transplantation
#2
Hyuk Jin Choi, Jiyeon Kim, Jae Young Kim, Hyun Ju Lee, Won Ryang Wee, Mee Kum Kim, Eung Soo Hwang
BACKGROUND: The risk of xenozoonosis mainly by porcine endogenous retrovirus (PERV) has been considered as one of the main hurdles in xenotransplantation and therefore should be elucidated prior to the clinical use of porcine corneal grafts. Accordingly, an investigation was performed to analyze the infectivity of PERVs from porcine keratocytes to human cells, and the long-term risk of transmission of PERVs was determined using pig-to-non-human primate (NHP) corneal transplantation models...
May 14, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28489286/sustained-activation-of-erk1-2-mapk-in-schwann-cells-causes-corneal-neurofibroma
#3
Paola Bargagna-Mohan, Akihiro Ishii, Ling Lei, Daniel Sheehy, Saagar Pandit, Grace Chan, Rashmi Bansal, Royce Mohan
Recent studies have shown that constitutive activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2) in Schwann cells (SCs) increases myelin thickness in transgenic mice. In this secondary analysis, we report that these transgenic mice develop a postnatal corneal neurofibroma with the loss of corneal transparency by age six months. We show that expansion of non-myelinating SCs, under the control of activated ERK1/2, also drive myofibroblast differentiation that derives from both SC precursors and resident corneal keratocytes...
May 10, 2017: Journal of Neuroscience Research
https://www.readbyqxmd.com/read/28486315/comparison-of-2-different-methods-of-transepithelial-corneal-collagen-cross-linking-analysis-of-corneal-histology-and-hysteresis
#4
Young Min Park, Ho Yoon Kim, Jong Soo Lee
PURPOSE: To compare the effect of 2 different methods of transepithelial corneal collagen cross-linking (CXL) on corneal histology and hysteresis. METHODS: The right eyes of New Zealand white rabbits (n = 50) were treated using one of the 2 transepithelial CXL methods. The eyes were then divided into 2 groups: group 1, transepithelial CXL using continuous ultraviolet A (UVA); group 2, accelerated transepithelial CXL using pulsed UVA. RESULTS: Both groups showed a postoperative increase in central corneal thickness...
May 8, 2017: Cornea
https://www.readbyqxmd.com/read/28485528/bioengineered-keratocyte-spheroids-fabricated-on-chitosan-coatings-enhance-tissue-repair-in-a-rabbit-corneal-stromal-defect-model
#5
Shih-Feng Chou, Chih-Hung Lee, Jui-Yang Lai
Cultivated cell spheroid transplantation is widely studied as a means of facilitating tissue regeneration. Chitosan biomaterial has been shown to promote keratocyte aggregation and multicellular spheroid formation. Herein, we provide further evidence on application of bioengineered keratocyte spheroids for corneal stromal tissue engineering. In an allogeneic rabbit model of stromal destruction caused by bacterial keratitis, the corneas were intrastromally injected with isolated keratocyte suspensions or aggregated spheroid grafts at same cell number...
May 9, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28458451/characteristics-of-corneal-phospholipidosis-induced-by-topical-ocular-application-of-chloroquine-and-amiodarone-in-rabbits
#6
Yoshinori Yamagiwa, Yu Haranosono, Shingo Nemoto, Ikuyo Atsumi, Masaaki Kurata, Gakushi Kito, Hirofumi Hatakeyama, Haruko Koizumi, Hideyuki Sakaki
Several cationic-amphiphilic drugs such as chloroquine and amiodarone are known to induce phospholipidosis in the cornea by systemic administration. However, the characteristics of ophthalmological and pathological changes when phospholipidosis-inducing drugs are topically applied have not been well studied. This study was conducted to investigate the characteristics of corneal changes caused by topical application of chloroquine and amiodarone to Japanese white rabbits. The changes were evaluated by ophthalmological, histopathological, and ultrastructural examinations...
April 2017: Journal of Toxicologic Pathology
https://www.readbyqxmd.com/read/28448563/corticosteroids-effects-on-lps-induced-rat-inflammatory-keratocyte-cell-model
#7
Huize Yan, Yingwei Wang, Shuhao Shen, Zheng Wu, Pengxia Wan
PURPOSE: Corticosteroids are efficient anti-inflammation treatments. However, there are still arguments on whether it should be used in keratitis. This study was to observe the effect of corticosteroids on keratocytes both in normal condition and inflammation status in vitro. METHODS: Rat keratocytes were cultured and used for examination. 10 μg/ml lipopolysaccharide (LPS) was used to establish the inflammatory keratocyte cell model, and prednisolone acetate (PA), dexamethasone (Dex) and fluorometholone (Flu) were used as corticosteroids treatments...
2017: PloS One
https://www.readbyqxmd.com/read/28442371/dynamites-a-dynamic-cell-culture-platform-for-in-vitro-drug-testing-part-1-engineering-of-microfluidic-system-and-technical-simulations
#8
Kai Mattern, Nicole Beißner, Stephan Reichl, Andreas Dietzel
Conventional safety and efficacy test models, such as animal experiments or static in vitro cell culture models, can often not reliably predict the most promising drug candidates. Therefore, a novel microfluidic cell culture platform, called Dynamic Micro Tissue Engineering System (DynaMiTES), was designed to allow online analysis of drugs permeating through barrier forming tissues under dynamic conditions combined with monitoring of the transepithelial electrical resistance (TEER) by electrodes optimized for homogeneous current distribution...
April 22, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28428541/tissue-engineered-cornea-constructed-with-compressed-collagen-and-laser-perforated-electrospun-mat
#9
Bin Kong, Wei Sun, Guoshi Chen, Song Tang, Ming Li, Zengwu Shao, Shengli Mi
While Plastic Compressed (PC) collagen technique is often used to fabricate bioengineered constructs, PC collagen gels are too weak to be sutured or conveniently handled for clinical applications. To overcome this limitation, electrospun poly (lactic-co-glycolide) (PLGA) mats, which have excellent biocompatibility and mechanical properties, were combined with PC collagen to fabricate sandwich-like hybrid constructs. By laser-perforating holes with different sizes and spacings in the electrospun mats to regulate the mechanical properties and light transmittance of the hybrid constructs, we produced hybrid constructs with properties very suitable to apply in corneal tissue engineering...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28410358/reactivation-of-herpes-zoster-keratitis-with-corneal-perforation-after-zoster-vaccination
#10
Andre Jastrzebski, Seymour Brownstein, Setareh Ziai, Solin Saleh, Kay Lam, W Bruce Jackson
PURPOSE: We present a case of reactivated herpes zoster keratouveitis of 6 years duration with corneal perforation requiring penetrating keratoplasty shortly after inoculation with herpes zoster vaccine (Zostavax, Merck, Quebec, Canada). METHODS: Retrospective case report. RESULTS: A 67-year-old woman with a 5-year history of recurrent unilateral herpes zoster keratouveitis in her right eye presented with another recurrence 2 weeks after Zostavax vaccination...
June 2017: Cornea
https://www.readbyqxmd.com/read/28393020/effects-of-exogenous-recombinant-human-bone-morphogenic-protein-7-on-the-corneal-epithelial-mesenchymal-transition-and-fibrosis
#11
Jin Kwon Chung, Shin Ae Park, Hee Sun Hwang, Kwang Sung Kim, Yang Je Cho, Yong Sung You, Young Sik Kim, Ju Woong Jang, Sung Jin Lee
AIM: To evaluate the effect of exogenous recombinant human bone morphogenic protein-7 (rhBMP-7) on transforming growth factor-β (TGF-β)-induced epithelial mesenchymal cell transition (EMT) and assessed its antifibrotic effect via topical application. METHODS: The cytotoxic effect of rhBMP-7 was evaluated and the EMT of human corneal epithelial cells (HECEs) was induced by TGF-β. HECEs were then cultured in the presence of rhBMP-7 and/or hyaluronic acid (HA). EMT markers, fibronectin, E-cadherin, α-smooth muscle actin (α-SMA), and matrix metaloproteinase-9 (MMP-9), were evaluated...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28384032/in-vitro-evaluation-of-the-ophthalmic-toxicity-profile-of-chlorhexidine-and-propamidine-isethionate-eye-drops
#12
Anxo Fernández-Ferreiro, María Santiago-Varela, María Gil-Martínez, Miguel González-Barcia, Andrea Luaces-Rodríguez, Victoria Díaz-Tome, María Pardo, José Blanco Méndez, Antonio Piñeiro-Ces, María Teresa Rodríguez-Ares, Maria Jesus Lamas, Francisco J Otero-Espinar
PURPOSE: Acanthamoeba keratitis causes frequent epithelial lesions that fully expose the corneal stroma. The aim of this study was to determine the toxic profile of chlorhexidine and propamidine eye drops. METHODS: We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. Additional studies such as a classic cell viability test (WST-1(®)), a bovine corneal opacity and permeability assay, and an irritation eye study (Hen's Egg Test [HET]) have been made...
April 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28366375/measuring-the-depth-of-crosslinking-demarcation-line-in%C3%A2-vivo-comparison-of-methods-and-devices
#13
Andreas Thorsrud, Gunhild F Sandvik, Anne M Hagem, Liv Drolsum
PURPOSE: To assess the interrelationship of different methods of measuring the demarcation line depth after corneal collagen crosslinking (CXL). SETTING: University eye clinic, Oslo, Norway. DESIGN: Prospective case series. METHODS: Eyes having CXL for progressive keratoconus were evaluated 1 month after CXL by in vitro confocal microscopy (IVCM), optical coherence tomography (OCT), and Scheimpflug imaging. When applying IVCM, the depth of the CXL demarcation line was measured with 2 methods; that is, IVCM keratocyte disappearance and IVCM intensity increase...
February 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28349952/nerve-regeneration-by-human-corneal-stromal-keratocytes-and-stromal-fibroblasts
#14
Gary Hin-Fai Yam, Geraint P Williams, Melina Setiawan, Nur Zahirah Binte M Yusoff, Xiao-Wen Lee, Hla Myint Htoon, Lei Zhou, Matthias Fuest, Jodhbir S Mehta
Laser refractive surgeries reshape corneal stroma to correct refractive errors, but unavoidably affect corneal nerves. Slow nerve regeneration and atypical neurite morphology cause desensitization and neuro-epitheliopathy. Following injury, surviving corneal stromal keratocytes (CSKs) are activated to stromal fibroblasts (SFs). How these two different cell types influence nerve regeneration is elusive. Our study evaluated the neuro-regulatory effects of human SFs versus CSKs derived from the same corneal stroma using an in vitro chick dorsal root ganglion model...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28346441/altering-the-threshold-of-an-excitable-signal-transduction-network-changes-cell-migratory-modes
#15
Yuchuan Miao, Sayak Bhattacharya, Marc Edwards, Huaqing Cai, Takanari Inoue, Pablo A Iglesias, Peter N Devreotes
The diverse migratory modes displayed by different cell types are generally believed to be idiosyncratic. Here we show that the migratory behaviour of Dictyostelium was switched from amoeboid to keratocyte-like and oscillatory modes by synthetically decreasing phosphatidylinositol-4,5-bisphosphate levels or increasing Ras/Rap-related activities. The perturbations at these key nodes of an excitable signal transduction network initiated a causal chain of events: the threshold for network activation was lowered, the speed and range of propagating waves of signal transduction activity increased, actin-driven cellular protrusions expanded and, consequently, the cell migratory mode transitions ensued...
April 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28339457/targeted-aav5-smad7-gene-therapy-inhibits-corneal-scarring-in-vivo
#16
Suneel Gupta, Jason T Rodier, Ajay Sharma, Elizabeth A Giuliano, Prashant R Sinha, Nathan P Hesemann, Arkasubhra Ghosh, Rajiv R Mohan
Corneal scarring is due to aberrant activity of the transforming growth factor β (TGFβ) signaling pathway following traumatic, mechanical, infectious, or surgical injury. Altered TGFβ signaling cascade leads to downstream Smad (Suppressor of mothers against decapentaplegic) protein-mediated signaling events that regulate expression of extracellular matrix and myogenic proteins. These events lead to transdifferentiation of keratocytes into myofibroblasts through fibroblasts and often results in permanent corneal scarring...
2017: PloS One
https://www.readbyqxmd.com/read/28302098/in-vivo-confocal-microscopy-of-pre-descemet-corneal-dystrophy-associated-with-x-linked-ichthyosis-a-case-report
#17
Hui Shi, Xiao-Feng Qi, Tao-Tao Liu, Qian Hao, Xiao-Hong Li, Ling-Ling Liang, Yi-Miao Wang, Zhi-Hua Cui
BACKGROUND: Pre-Descemet corneal dystrophy (PDCD) is characterized by the presence of numerous, tiny, polymorphic opacities immediately anterior to Descemet membrane, which is a rare form of corneal stromal dystrophy and hard to be diagnosed. In vivo confocal microscopy (IVCM) is a useful tool to examine the minimal lesions of the cornea at the cellular level. In this article, we report a rare case of PDCD associated with X-linked ichthyosis and evaluate IVCM findings. CASE PRESENTATION: We present a 34-year-old male Chinese patient with PDCD associated with X-linked ichthyosis...
March 16, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28301610/the-effects-of-substance-p-and-acetylcholine-on-human-tenocyte-proliferation-converge-mechanistically-via-tgf-%C3%AE-1
#18
Gloria Fong, Ludvig J Backman, Håkan Alfredson, Alex Scott, Patrik Danielson
Previous in vitro studies on human tendon cells (tenocytes) have demonstrated that the exogenous administration of substance P (SP) and acetylcholine (ACh) independently result in tenocyte proliferation, which is a prominent feature of tendinosis. Interestingly, the possible link between SP and ACh has not yet been explored in human tenocytes. Recent studies in other cell types demonstrate that both SP and ACh independently upregulate TGF-β1 expression via their respective receptors, the neurokinin 1 receptor (NK-1R) and muscarinic ACh receptors (mAChRs)...
2017: PloS One
https://www.readbyqxmd.com/read/28273315/extracellular-collagen-promotes-interleukin-1%C3%AE-induced-urokinase-type-plasminogen-activator-production-by-human-corneal-fibroblasts
#19
Koji Sugioka, Aya Kodama-Takahashi, Koji Yoshida, Keiichi Aomatsu, Kiyotaka Okada, Teruo Nishida, Yoshikazu Shimomura
Purpose: Keratocytes maintain homeostasis of the corneal stroma through synthesis, secretion, and degradation of collagen fibrils of the extracellular matrix. Given that these cells are essentially embedded in a collagen matrix, keratocyte-collagen interactions may play a key role in regulation of the expression or activation of enzymes responsible for matrix degradation including urokinase-type plasminogen activator (uPA), plasmin, and matrix metalloproteinases (MMPs). We examined the effect of extracellular collagen on the production of uPA by corneal fibroblasts (activated keratocytes) stimulated with the proinflammatory cytokine interleukin-1β (IL-1β)...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#20
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
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