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https://www.readbyqxmd.com/read/29772228/3d-bioprinting-of-a-corneal-stroma-equivalent
#1
Abigail Isaacson, Stephen Swioklo, Che J Connon
Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. However, successful translation of these therapies into the clinic has not yet been accomplished. 3D bioprinting is an emerging technology that can be harnessed for the fabrication of biological tissue for clinical applications. We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure...
May 14, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29771882/-morphological-changes-of-cornea-in-children-with-hyperopia-in-the-immediate-and-remote-postoperative-period-after-laser-keratomieleusis-according-to-confocal-microscopy-data
#2
I L Kulikova, N P Pashtaev, O V Shlenskaya
Studying morphological changes of cornea in the remote postoperative period is becoming increasingly relevant with refractive surgeries being adapted for children. PURPOSE: comparative analysis of keratocyte density and hystomorphologic changes of cornea after laser in situ keratomieleusis (LASIK) and femtosecond laser-assisted LASIK (FS-LASIK) in children with hyperopia. MATERIAL AND METHODS: 109 patients aged 6-17 years in 2 groups were examined with 'Confoscan-4' confocal microscope...
2018: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/29728753/heredity-and-in-vivo-confocal-microscopy-of-punctiform-and-polychromatic-pre-descemet-dystrophy
#3
María Angélica Henríquez Recine, Kelly Sonia Marquina Lima, Elena Vallespín García, Sixto García-Miñaur, José Manuel Benitez Del Castillo, Ana Boto de Los Bueis
PURPOSE: To describe and analyze the biomicroscopic features and in vivo confocal microscopy of the crystalline form of pre-Descemet corneal dystrophy (PDCD). METHODS: We examined two non-related families using biomicroscopy, in vivo confocal microscopy, and a genetic study using a gene panel test, looking for mutations in the PIKFYVE gene. RESULTS: A slit-lamp examination of the first family revealed polychromatic crystalline punctiform opacities distributed all over the stroma in 8 of 11 family members in three generations with an autosomal dominant inheritance...
May 4, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29714695/the-role-of-dental-care-providers-in-the-management-of-patients-prescribed-bisphosphonates-brief-clinical-guidance
#4
Hassan H Abed, Elham N Al-Sahafi
Dental care providers are likely to see patients who take bisphosphonates for various medical conditions, including osteoporosis, bony metastatic tumors, multiple myeloma, breast cancer, and prostate cancer. Bisphosphonates accumulate in areas of high bone turnover, leading to suppression of bone turnover and the aging of keratocytes. These adverse effects predispose the maxillary and mandibular bone to development of medication-related osteonecrosis of the jaw (MRONJ), specifically among oncology patients treated with intravenous bisphosphonate therapy...
May 2018: General Dentistry
https://www.readbyqxmd.com/read/29703291/continuous-light-versus-pulsed-light-accelerated-corneal-crosslinking-with-ultraviolet-a-and-riboflavin
#5
Yirui Zhu, Peter S Reinach, Hanlei Zhu, Ling Li, Fan Yang, Jia Qu, Wei Chen
PURPOSE: To determine whether the pulsed-light ultraviolet-A (UVA) accelerated corneal crosslinking (CXL) procedure is more efficacious and selective than its continuous-light counterpart in rabbits. SETTING: School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China. DESIGN: Experimental study. METHODS: Fifty-four rabbits were divided into 2 groups. Group 1 had continuous-light accelerated CXL using 9 mW/cm2 UVA for 10 minutes (5...
March 2018: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29673063/uv-light-crosslinking-regresses-mature-corneal-blood-and-lymphatic-vessels-and-promotes-subsequent-high-risk-corneal-transplant-survival
#6
Yanhong Hou, Viet Nhat Hung Le, Gábor Tóth, Sebastian Siebelmann, Jens Horstmann, Tim Gabriel, Felix Bock, Claus Cursiefen
Immunological graft rejection is the main complication after corneal transplantation into pathologically prevascularized so-called high risk eyes. Aim of this study was to evaluate whether UV-light crosslinking can regress pathological corneal blood and lymphatic vessels, and thereby improve subsequent graft survival. Using the murine model of suture-induced corneal neovascularization, we found that corneal crosslinking with ultraviolet (UV) A-light and riboflavin regressed both preexisting blood and lymphatic vessels significantly via inducing apoptosis in vascular endothelial cells...
April 19, 2018: American Journal of Transplantation
https://www.readbyqxmd.com/read/29653143/phenotypic-characterization-of-the-sirc-statens-seruminstitut-rabbit-cornea-cell-line-reveals-a-mixed-epithelial-and-fibroblastic-nature
#7
Melania Olivieri, Martina Cristaldi, Salvatore Pezzino, Dario Rusciano, Barbara Tomasello, Carmelina Daniela Anfuso, Gabriella Lupo
The aim of the present study was to investigate, in the Statens Seruminstitut Rabbit Cornea (SIRC) cell line, the presence of epithelial and fibroblastic markers, comparing their levels with those of the human Retinal Pigmented Epithelial (ARPE-19) cell line, and the Human Keratocyte (HK) cell line, respectively. SIRC cells, often described as of epithelial origin, are used as a corneal epithelial barrier model to study the permeability of ophthalmic drugs. However, they show a morphology that is more consistent with a fibroblastic cell phenotype, similar to corneal keratocytes...
April 10, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29596850/posterior-stromal-cell-apoptosis-triggered-by-mechanical-endothelial-injury-and-basement-membrane-component-nidogen-1-production-in-the-cornea
#8
Carla S Medeiros, Luciana Lassance, Paramananda Saikia, Marcony Santhiago, Steven E Wilson
This study was performed to determine whether cells in the posterior stroma undergo apoptosis in response to endothelial cell injury and to determine whether basement membrane component nidogen-1 was present in the cornea. New Zealand White rabbits had an olive tip cannula inserted into the anterior chamber to mechanically injure corneal endothelial cells over an 8 mm diameter area of central cornea with minimal injury to Descemet's membrane. At 1 h (6 rabbits) and 4 h (6 rabbits) after injury, three corneas at each time point were cryopreserved in OCT for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunohistochemistry (IHC) for vimentin and nidogen-1, and three corneas at each time point were fixed for transmission electron microscopy (TEM)...
March 26, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29575612/assessment-of-the-metabolism-and-morphology-of-the-porcine-cornea-lens-and-retina-by-two-photon-imaging
#9
Ana Batista, Hans Georg Breunig, Aisada König, António Miguel Morgado, Karsten König
Two-photon imaging is a non-invasive imaging technique with increasing importance in the biological and medical fields since it allows intratissue cell imaging with high resolution. We demonstrate the feasibility of using a single two-photon instrument to evaluate the cornea, the crystalline lens, and the retina based on their autofluorescence. Image acquisition was performed using a custom-built two-photon microscope for 5D microscopy with a near infrared broadband sub-15 femtosecond laser centered at 800 nm...
March 25, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29563392/morphometric-analysis-of-cornea-in-the-slc39a13-zip13-knockout-mice
#10
Takuya Hirose, Ippei Suzuki, Naoki Takahashi, Toshiyuki Fukada, Prasarn Tangkawattana, Kazushige Takehana
Ehlers-Danlos syndrome (EDS) is a group of hereditary diseases caused by mutation of extracellular matrix-related genes. Recently, spondylodysplastic EDS-Zip13 (spEDS-Zip13: OMIM 612350) was recognized as a new EDS type. This current study could reveal various morphometric differences of collagenous population in the proper substance of cornea between the wild type and spEDS-Zip13-knockout (Zip13-KO) mice. Blockade of Smad-signaling pathway might initiate these alterations. Predilected dissimilarity in level of transcriptional activity probably dictated morphology of keratocyte and shape and electron density of its nucleus...
March 22, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29552393/-in-vivo-high-resolution-human-corneal-imaging-using-full-field-optical-coherence-tomography
#11
Viacheslav Mazlin, Peng Xiao, Eugénie Dalimier, Kate Grieve, Kristina Irsch, José-Alain Sahel, Mathias Fink, A Claude Boccara
We present the first full-field optical coherence tomography (FFOCT) device capable of in vivo imaging of the human cornea. We obtained images of the epithelial structures, Bowman's layer, sub-basal nerve plexus (SNP), anterior and posterior stromal keratocytes, stromal nerves, Descemet's membrane and endothelial cells with visible nuclei. Images were acquired with a high lateral resolution of 1.7 µm and relatively large field-of-view of 1.26 mm x 1.26 mm - a combination, which, to the best of our knowledge, has not been possible with other in vivo human eye imaging methods...
February 1, 2018: Biomedical Optics Express
https://www.readbyqxmd.com/read/29550185/in-vivo-confocal-microscopy-cellular-features-of-host-and-organism-in-bacterial-fungal-and-acanthamoeba-keratitis
#12
Jaya D Chidambaram, Namperumalsamy V Prajna, Srikanthi Palepu, Shruti Lanjewar, Manisha Shah, Shanmugam Elakkiya, David Macleod, Prajna Lalitha, Matthew J Burton
PURPOSE: To determine cellular features of fungal (FK), Acanthamoeba (AK) and bacterial keratitis (BK) using HRT3 in vivo confocal microscopy (IVCM). DESIGN: Prospective observational cross-sectional study. METHODS: Eligible participants were adults with microbiologically-positive FK, AK or BK, of size ≥3mm attending Aravind Eye Hospital from Feb 2012 to Feb 2013. EXCLUSION CRITERIA: descemetocele, perforation. At presentation, IVCM imaging was performed, then corneal scrapes obtained for culture/light microscopy...
March 14, 2018: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29536619/postnatal-periodontal-ligament-as-a-novel-adult-stem-cell-source-for-regenerative-corneal-cell-therapy
#13
Gary Hin-Fai Yam, Ericia Pei-Wen Teo, Melina Setiawan, Matthew J Lovatt, Nur Zahirah Binte M Yusoff, Matthias Fuest, Bee-Tin Goh, Jodhbir S Mehta
Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest...
March 13, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29530529/lamellipodial-wrinkles-in-fish-keratocytes-as-markers-of-imperfect-coordination-between-extension-and-retraction-during-cell-migration
#14
Miguel Arocena, Jorge M Pérez Zerpa, Andrés Di Paolo, Pedro A Aguilera, José Sotelo-Silveira
Cell migration involves the precise coordination between extension at the front of the cell and retraction at the rear. This coordination is particularly evident in fast moving cells such as fish keratocytes, where it leads to highly stable gliding motion, propelled at the front by broad, 0.1-0-2 μm thick lamellipodia. Transient uncoupling between extension and retraction can occur if the rear is temporarily stuck, which might eventually lead to cell shape instabilities. We have frequently observed in fish keratocytes the presence of lamellipodial radial wrinkles, detected by confocal, scanning electron and side-view microscopy as folds in the lamellipodium up to 2 μm in height...
April 6, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29528760/the-effect-of-antiamoebic-agents-on-viability-proliferation-and-migration-of-human-epithelial-cells-keratocytes-and-endothelial-cells-in-vitro
#15
Lei Shi, Tanja Stachon, Berthold Seitz, Stefan Wagenpfeil, Achim Langenbucher, Nóra Szentmáry
PURPOSE: To analyze the effect of diamidines (hexamidine-diisethionat (HD), propamidin-isethionate (PD), dibromopropamidine-diisethionat (DD)), and biguanides (polyhexamethylen biguanid (PHMB), chlorhexidine (CH)) on human corneal epithelial cell, keratocyte and endothelial cell viability, proliferation, and migration, in vitro. METHODS: For epithelial and endothelial cells a human cell line and for keratocytes primary cultures were used (n = 6 each). We used 3...
June 2018: Current Eye Research
https://www.readbyqxmd.com/read/29521690/confocal-microscopy-observation-of-cornea-verticillata-after-vandetanib-therapy-for-medullary-thyroid-carcinoma
#16
Pedro Arriola-Villalobos, Blanca Benito-Pascual, David Díaz-Valle, José M Benítez-Del-Castillo
PURPOSE: To report 2 cases of cornea verticillata (CV) after vandetanib treatment for medullary thyroid carcinoma (MTC). METHODS: In this retrospective interventional, case-report study, 2 patients who under vandetanib treatment for MTC were referred to our ophthalmology department because of vision complaints. Both subjects underwent a complete ophthalmologic examination, including confocal microscopy (CM) using the Heidelberg Retina Tomograph and Rostock Cornea Module...
June 2018: Cornea
https://www.readbyqxmd.com/read/29495264/engineering-of-corneal-tissue-through-an-aligned-pva-collagen-composite-nanofibrous-electrospun-scaffold
#17
Zhengjie Wu, Bin Kong, Rui Liu, Wei Sun, Shengli Mi
Corneal diseases are the main reason of vision loss globally. Constructing a corneal equivalent which has a similar strength and transparency with the native cornea, seems to be a feasible way to solve the shortage of donated cornea. Electrospun collagen scaffolds are often fabricated and used as a tissue-engineered cornea, but the main drawback of poor mechanical properties make it unable to meet the requirement for surgery suture, which limits its clinical applications to a large extent. Aligned polyvinyl acetate (PVA)/collagen (PVA-COL) scaffolds were electrospun by mixing collagen and PVA to reinforce the mechanical strength of the collagen electrospun scaffold...
February 24, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29439091/evaluation-of-galectin-1-and-galectin-3-as-prospective-biomarkers-in-keratoconus
#18
Frans Eberth Costa Andrade, Joyce Luciana Covre, Lisandra Ramos, Rossen Mihaylov Hazarbassanov, Myrna Serapião Dos Santos, Mauro Campos, José Álvaro Pereira Gomes, Cristiane Damas Gil
AIMS: To evaluate the expression of β-galactoside-binding proteins galectin (Gal)-1 and Gal-3 in patients with keratoconus (KC) and postcorneal collagen cross-linking (CXL) treatment in vitro. METHODS: Tear fluid, cornea samples and conjunctival impression cytology specimens from control and KC patients were used to evaluate Gal-1 and Gal-3 expressions. Primary keratocytes were isolated by collagenase digestion from surgically removed corneas of five normal or KC human corneal buttons and cultured in Dulbecco's modified eagle medium/Ham's F12 medium supplemented with 2% fetal bovine serum...
May 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29434243/isolation-and-expansion-of-multipotent-progenitors-from-human-trabecular-meshwork
#19
Yuan Zhang, Subo Cai, Scheffer C G Tseng, Ying-Ting Zhu
To expand multi-potent progenitors from human trabecular meshwork (TM), we have created a new optimized method on two-dimensional (2D) followed by three-dimensional (3D) Matrigel in modified embryonic stem cell medium supplemented with 5% fetal bovine serum (MESCM + 5% FBS). The expanded TM cells were small cuboidal cells expressing TM markers such as AQP1, MGP, CHI3L1, and AnkG, embryonic stem cell (ESC) markers such as Oct4, Sox2, Nanog, and ABCG2, and neural crest (NC) markers such as p75NTR, FOXD3, Sox9, Sox10, and MSX1...
February 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29421383/latrunculin-b-and-substratum-stiffness-regulate-corneal-fibroblast-to-myofibroblast-transformation
#20
Sara M Thomasy, Vijay Krishna Raghunathan, Hidetaka Miyagi, Alexander T Evashenk, Jasmyne C Sermeno, Geneva K Tripp, Joshua T Morgan, Christopher J Murphy
The transformation of keratocytes and fibroblasts to myofibroblasts is important to corneal wound healing as well as formation of stromal haze. The purpose of this study was to determine the effect of latrunculin B, an actin cytoskeleton disruptor in conjunction with a fundamental biophysical cue, substrate stiffness, on myofibroblast transformation in vitro and in vivo. Rabbit corneal fibroblasts were cultured on substrates of differing compliance (1.5, 22, and 71 kPa) and tissue culture plastic (TCP; > 1 GPa) in media containing 0 or 10 ng/ml TGFβ1 for 72 h...
May 2018: Experimental Eye Research
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