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https://www.readbyqxmd.com/read/29149196/easy-xeno-free-and-feeder-free-method-for-isolating-and-growing-limbal-stromal-and-epithelial-stem-cells-of-the-human-cornea
#1
Djida Ghoubay-Benallaoua, Céline de Sousa, Raphaël Martos, Gaël Latour, Marie-Claire Schanne-Klein, Elisabeth Dupin, Vincent Borderie
Epithelial and stromal stem cells are required to maintain corneal transparency. The aim of the study was to develop a new method to isolate and grow both corneal stromal (SSC) and epithelial limbal (LSC) stem cells from small human limbal biopsies under culture conditions in accordance with safety requirements mandatory for clinical use in humans. Superficial limbal explants were retrieved from human donor corneo-scleral rims. Human limbal cells were dissociated by digestion with collagenase A, either after epithelial scraping or with no scraping...
2017: PloS One
https://www.readbyqxmd.com/read/29134127/the-characterization-of-human-oral-mucosal-fibroblasts-and-their-use-as-feeder-cells-in-cultivated-epithelial-sheets
#2
Kazunari Higa, Yoshiyuki Satake, Jun Shimazaki
Aim: To characterize human oral mucosa middle interstitial tissue fibroblasts (hOMFs) and their application in the cultivation of epithelial sheets. Methodology: hOMFs were cultured with methylcellulose to form cell clusters. hOMFs amplified in adhesive culture were analyzed by flow cytometry, and were found to differentiate into multiple cell types suitable for the cultivation of human corneal epithelial sheets. hOMFs were expanded from clusters to analyze CD56 and PDGFRα expression...
November 2017: Future Science OA
https://www.readbyqxmd.com/read/29132420/substance-p-and-patterned-silk-biomaterial-stimulate-periodontal-ligament-stem-cells-to-form-corneal-stroma-in-a-bioengineered-three-dimensional-model
#3
Jialin Chen, Wei Zhang, Peyman Kelk, Ludvig J Backman, Patrik Danielson
BACKGROUND: We aimed to generate a bioengineered multi-lamellar human corneal stroma tissue in vitro by differentiating periodontal ligament stem cells (PDLSCs) towards keratocytes on an aligned silk membrane. METHODS: Human PDLSCs were isolated and identified. The neuropeptide substance P (SP) was added in keratocyte differentiation medium (KDM) to evaluate its effect on keratocyte differentiation of PDLSCs. PDLSCs were then seeded on patterned silk membrane and cultured with KDM and SP...
November 13, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29128534/development-of-gelatin-ascorbic-acid-cryogels-for-potential-use-in-corneal-stromal-tissue-engineering
#4
Li-Jyuan Luo, Jui-Yang Lai, Shih-Feng Chou, Yi-Jen Hsueh, David Hui-Kang Ma
To offer an ideal hospitable environment for corneal keratocyte growth, the carrier materials can be functionalized with incorporation of signaling molecules to regulate cell biological events. This study reports, for the first time, the development of gelatin/ascorbic acid (AA) cryogels for keratocyte carriers in vitro and in vivo. The cryogel samples were fabricated by blending of gelatin with varying amounts of AA (0-300 mg) and carbodiimide cross-linking via cryogelation technique. Hydrophilic AA content in the carriers was found to significantly affect cross-linking degree and pore dimension of cryogels, thereby dictating their mechanical and biological stability and AA release profile...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29123364/corneal-lenticule-storage-before-reimplantation
#5
Yu-Chi Liu, Geraint P Williams, Ben L George, Yu Qiang Soh, Xin Yi Seah, Gary Swee Lim Peh, Gary Hin Fai Yam, Jodhbir S Mehta
Purpose: To explore the optimal lenticule storage conditions that maintain lenticule integrity and clarity. Methods: A total of 99 lenticules obtained from myopic patients undergoing small incision lenticule extraction (SMILE) were divided into four combinations for short-term storage conditions: PBS, Dulbecco's Modified Eagle's Medium (DMEM), Optisol GS, or anhydrous glycerol. Two thirds of the lenticules were further stored for 4 weeks under eight different conditions...
2017: Molecular Vision
https://www.readbyqxmd.com/read/29120718/wound-healing-in-rabbit-corneas-after-flapless-refractive-lenticule-extraction-with-a-345%C3%A2-nm-ultraviolet-femtosecond-laser
#6
Christian M Hammer, Corinna Petsch, Jörg Klenke, Katrin Skerl, Christian Wüllner, Christof Donitzky, Friedrich Paulsen, Michael Scholz, Theo Seiler, Friedrich E Kruse, Johannes Menzel-Severing
PURPOSE: To characterize corneal wound healing in a rabbit model after flapless refractive lenticule extraction with a 345 nm ultraviolet femtosecond laser. SETTING: Departments of Ophthalmology and Anatomy II, University of Erlangen-Nürnberg and Wavelight GmbH, Erlangen, Germany. DESIGN: Experimental study. METHODS: Flapless refractive lenticule extraction was performed in 1 eye each of 20 New Zealand white rabbits (-5...
October 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/29117318/lamin-cleavage-a-reliable-marker-for-studying-staurosporine-induced-apoptosis-in-corneal-tissue
#7
Siddharth Mahajan, Daniel Thieme, Marta Czugala, Friedrich E Kruse, Thomas A Fuchsluger
Purpose: The aims of this study were to identify a robust apoptosis marker suitable for both quantification and back-to-back analyses of programmed cell death and to define specific upstream targets for apoptosis in corneal cells. Methods: Apoptotic cleavage of initiator caspases and their downstream targets such as lamins and poly-ADP ribose polymerase was investigated in human corneal endothelial cells (HCEC-12), keratocytes (HCK), epithelial cells (HCEp), and full-thickness corneas using Western blotting and confocal microscopy following apoptosis induction with staurosporine...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29111817/structural-ultrastructural-and-morphometric-study-of-the-zebrafish-ocular-surface-a-model-for-human-corneal-diseases
#8
Domenico Puzzolo, Antonina Pisani, Consuelo Malta, Giuseppe Santoro, Alessandro Meduri, Francesco Abbate, Giuseppe Montalbano, Edward Wylegala, Rosa Alba Rana, Fabio Bucchieri, Antonio Ieni, Pasquale Aragona, Antonio Micali
PURPOSE: A morphological and morphometric study of the adult zebrafish ocular surface was performed to provide a comprehensive description of its parts and to evaluate its similarity to the human. MATERIALS AND METHODS: The eyes of adult zebrafish were processed for light, transmission and scanning electron microscopy, and for immunohistochemical stain of corneal nerves; a morphometric analysis was also performed on several morphological parameters. RESULTS: The corneal epithelium was formed by five layers of cells...
November 7, 2017: Current Eye Research
https://www.readbyqxmd.com/read/29067107/conventional-vs-pulsed-light-accelerated-corneal-collagen-cross-linking-for-the-treatment-of-progressive-keratoconus-12-month-results-from-a-prospective-study
#9
Liang-Zhu Jiang, Wei Jiang, Shi-Yan Qiu
The aim of the present study was to compare the clinical outcomes of conventional corneal collagen cross-linking (CXL) and pulsed-light accelerated CXL (pl-ACXL) in the eyes of patients with progressive keratoconus. A total of 72 eyes with progressive keratoconus in 58 patients were equally divided into the CXL and pl-ACXL treatment groups. The CXL treatment was performed using the UVX 1000 system with 0.1% riboflavin solution in 20% dextran presoak for 30 min, and 3 mW/cm(2) ultraviolet A (UVA) light for 30 min...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29024376/evaluation-of-collagen-foam-poly-l-lactic-acid-nanofiber-mesh-and-decellularized-matrices-for-corneal-regeneration
#10
Bahar Aslan, Selcan Guler, Atakan Tevlek, Halil Murat Aydin
Corneal tissue engineering efforts to obtain corneal tissue matrices through various types of materials for the replacement of damaged tissues. In this study, three different corneal constructs were prepared and evaluated in terms of morphological, optical, and biological characteristics. Type-I collagen was used to obtain collagen foam scaffolds through dehydrothermal crosslinking, while poly(l-lactic acid) (PLLA) was used to produce both random and aligned oriented electrospun corneal constructs. Bovine corneas were decellularized as third matrix...
October 12, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28973427/histopathological-and-molecular-changes-in-the-rabbit-cornea-from-arsenical-vesicant-lewisite-exposure
#11
Neera Tewari-Singh, Dinesh G Goswami, Rama Kant, David A Ammar, Dileep Kumar, Robert W Enzenauer, Robert P Casillas, Claire R Croutch, J Mark Petrash, Rajesh Agarwal
Lewisite (LEW), a potent arsenical vesicating chemical warfare agent, poses a continuous risk of accidental exposure in addition to its feared use as a terrorist weapon. Ocular tissue is exquisitely sensitive to LEW and exposure can cause devastating corneal lesions. However, detailed pathogenesis of corneal injury and related mechanisms from LEW exposure that could help identify targeted therapies are not available. Using an established consistent and efficient exposure system, we evaluated the pathophysiology of the corneal injury in New-Zealand white rabbits following LEW vapor exposure (at 0...
September 18, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28973326/generation-and-characterization-of-a-novel-mouse-line-keratocan-rtta-kerart-for-corneal-stroma-and-tendon-research
#12
Yujin Zhang, Winston W-Y Kao, Yasuhito Hayashi, Lingling Zhang, Mindy Call, Fei Dong, Yong Yuan, Jianhua Zhang, Yen-Chiao Wang, Okada Yuka, Atsushi Shiraishi, Chia-Yang Liu
Purpose: We created a novel inducible mouse line Keratocan-rtTA (KeraRT) that allows specific genetic modification in corneal keratocytes and tenocytes during development and in adults. Methods: A gene-targeting vector (Kera- IRES2-rtTA3) was constructed and inserted right after the termination codon of the mouse Kera allele via gene targeting techniques. The resulting KeraRT mouse was crossed to tet-O-Hist1H2B-EGFP (TH2B-EGFP) to obtain KeraRT/TH2B-EGFP compound transgenic mice, in which cells expressing Kera are labeled with green fluorescence protein (GFP) by doxycycline (Dox) induction...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28968292/aberrant-patterns-of-key-epithelial-basement-membrane-components-in-keratoconus
#13
Carol Ann Greene, Charisse Kuo, Trevor Sherwin
PURPOSE: In the cornea, the epithelial basement membrane (BM) plays an important role in maintaining corneal integrity and homeostasis. Aberrations in this vital structure are associated with several corneal pathologies including keratoconus. The aim of this study was to investigate the expression of key structural components of the epithelial BM in keratoconic corneas and to identify and describe any aberrant patterns. METHODS: Immunohistochemical labeling of key BM components including fibronectin, laminin, and type IV and VII collagen was performed in healthy and keratoconic corneas...
December 2017: Cornea
https://www.readbyqxmd.com/read/28966086/actin-turnover-in-lamellipodial-fragments
#14
Dikla Raz-Ben Aroush, Noa Ofer, Enas Abu-Shah, Jun Allard, Oleg Krichevsky, Alex Mogilner, Kinneret Keren
Actin turnover is the central driving force underlying lamellipodial motility. The molecular components involved are largely known, and their properties have been studied extensively in vitro. However, a comprehensive picture of actin turnover in vivo is still missing. We focus on fragments from fish epithelial keratocytes, which are essentially stand-alone motile lamellipodia. The geometric simplicity of the fragments and the absence of additional actin structures allow us to characterize the spatiotemporal lamellipodial actin organization with unprecedented detail...
October 9, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28965357/in-vivo-confocal-microscopic-investigation-of-the-cornea-after-autologous-implantation-of-lenticules-obtained-through-small-incision-lenticule-extraction-for-treatment-of-hyperopia
#15
Meiyan Li, Meng Li, Ling Sun, Tian Han, Lan Ding, Jun Xiang, Xingtao Zhou
PURPOSE: To investigate re-innervation in the implanted lenticule, as well as changes to the cornea, after correcting hyperopia with an autologous implantation of a lenticule obtained through small incision lenticule extraction (SMILE). METHODS: This study retrospectively analysed re-innervation in the implanted lenticule, as well as microscopic morphological changes in the corneal architecture of the recipient cornea in five patients (with myopia in one eye and hyperopia in the contralateral eye) who received SMILE in the myopic eye and femtosecond laser in situ keratomileusis (FS-LASIK) combined with lenticule implantation in the contralateral hyperopic eye...
October 1, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/28963702/in-vivo-confocal-laser-microscopy-of-morphologic-changes-after-small-incision-lenticule-extraction-with-accelerated-cross-linking-smile-xtra-in-patients-with-thin-corneas-and-high-myopia
#16
Yugui Zhou, Manli Liu, Ting Zhang, Hua Zheng, Yuan Sun, Xiaonan Yang, Shengbei Weng, Haiqin Lin, Quan Liu
PURPOSE: To evaluate the microstructural modifications and safety of small incision lenticule extraction combined with accelerated cross-linking (SMILE Xtra) in high myopia and thin corneas by means of in vivo confocal microscopy (IVCM) and 3D-OCT after a 6-month follow-up. METHODS: Forty-three eyes with high myopia and thin corneas were enrolled. All eyes underwent SMILE procedure. After the lenticule was extracted, 0.25% riboflavin was injected into the interface and allowed to diffuse for 60 s...
September 29, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28936615/procollagen-c-proteinase-enhancer-1-pcpe-1-functions-as-an-anti-angiogenic-factor-and-enhances-epithelial-recovery-in-injured-cornea
#17
Dawiyat Massoudi, Colin J Germer, Jeffrey M Glisch, Daniel S Greenspan
Procollagen C-proteinase enhancer 1 (PCPE-1) has been characterized as a protein capable of enhancing the activity of bone morphogenetic protein 1/tolloid-like proteinases in the biosynthetic processing of C-propeptides from procollagens I-III. This processing step is thought necessary to the formation of collagen I-III monomers capable of forming fibrils. Thus, PCPE-1 is predicted to play an important role in scarring, as scar tissue is predominantly composed of fibrillar collagen. Corneal scarring is of great clinical importance, as it leads to loss of visual acuity and, in severe cases, blindness...
September 21, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28936480/the-role-of-corneal-stroma-a-potential-nutritional-source-for-the-cornea
#18
Lingling Zhang, Matthew C Anderson, Chia-Yang Liu
Corneal stroma plays a pivotal role in normal visual function. Anatomically, it is located between the outer epithelium and the inner endothelium and is the thickest layer of the cornea. Keratocytes in the stroma produce a variety of cellular products, including growth factors/cytokines, extracellular matrix (ECM) components, and kinases. These products support normal corneal development and homeostasis.
August 2017: Journal of Nature and Science
https://www.readbyqxmd.com/read/28932340/contact-lenses-for-keratoconus-current-practice
#19
Marilita M Moschos, Eirini Nitoda, Panagiotis Georgoudis, Miltos Balidis, Eleftherios Karageorgiadis, Nikos Kozeis
BACKGROUND: Keratoconus is a chronic, bilateral, usuallly asymmetrical, non-inflammatory, ectatic disorder, being characterized by progressive steepening, thinning and apical scarring of the cornea. Initially, the patient is asymptomatic, but the visual acuity gradually decreases, resulting in significant vision loss due to the development of irregular astigmatism, myopia, corneal thinning and scarring. The classic treatment of visual rehabilitation in keratoconus is based on spectacles and contact lenses (CLs)...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28913508/confocal-biomicroscopic-changes-of-the-corneal-layers-following-femtosecond-laser-assisted-myoring-implantation-in-keratoconus
#20
Heidar Siatiri, Mahmoud Jabbarvand, Mehrdad Mohammadpour, Amirhossein Mollazadeh, Nasim Siatiri, Arash Mirmohammadsadeghi
PURPOSE: To evaluate the effect of the femtosecond laser-assisted MyoRing implantation on the confocal biomicroscopic findings in different corneal layers of the patients with keratoconus. METHODS: Twelve eyes of 12 patients with mild to moderate keratoconus (keratometry between 48 and 52 diopters) and intolerance to hard contact lens entered the study. All the included patients underwent femtosecond laser-assisted MyoRing (Dioptex GmBH, Linz, Austria) implantation...
September 2017: Journal of Current Ophthalmology
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