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https://www.readbyqxmd.com/read/27818315/il-20-promotes-epithelial-healing-of-the-injured-mouse-cornea
#1
Wanyu Zhang, Sri Magadi, Zhijie Li, C Wayne Smith, Alan R Burns
After corneal epithelial injury, the ensuing inflammatory response is necessary for efficient wound healing. While beneficial healing effects are attributed to recruited neutrophils and platelets, dysregulated inflammation (too little or too much) is associated with impaired wound healing. The purpose of this study was to use an established C57BL/6J mouse model of corneal injury to evaluate the potential modulatory role of interleukin-20 (IL-20) on the inflammatory and healing responses to epithelial wounding...
November 3, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27815483/effect-of-autologous-serum-eye-drops-in-patients-with-sj%C3%A3-gren-syndrome-related-dry-eye-clinical-and-in-vivo-confocal-microscopy-evaluation-of-the-ocular-surface
#2
Francesco Semeraro, Eliana Forbice, Giuseppe Nascimbeni, Marco Taglietti, Vito Romano, Germano Guerra, Ciro Costagliola
AIM: To evaluate in vivo changes after therapy using autologous serum (AS) eye drops in Sjögren's syndrome (SS)-related dry eyes by confocal microscopy. PATIENTS AND METHODS: In this study, 24 patients with SS-related dry eyes [12 in AS eye drop therapy and 12 in artificial tear (AT) therapy] and 24 healthy volunteers were recruited. Ocular Surface Disease Index (OSDI), central corneal thickness, tear film, break-up time, corneal and conjunctival staining, Schirmer's test and corneal confocal microscopy were investigated...
November 2016: In Vivo
https://www.readbyqxmd.com/read/27802519/antiapoptotic-effect-of-acetylcholine-in-fas-induced-apoptosis-in-human-keratocytes
#3
Marta Sloniecka, Ludvig J Backman, Patrik Danielson
Purpose: To investigate the possible antiapoptotic effect of acetylcholine (ACh) in Fas-mediated apoptosis of primary human keratocytes in vitro, and to explore the underlying mechanism. Methods: Primary human keratocytes were isolated from healthy corneas. Fas ligand (FasL) was used to induce apoptosis in keratocytes. Cell death was assessed by ELISA. Activity of caspase-3, -7, -8, and -9 was measured with luminescent caspase activity assays. Expression of nuclear factor-κB (NF-κB) gene was assessed with RT-quantitative (q)PCR...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27792791/derivation-of-corneal-keratocyte-like-cells-from-human-induced-pluripotent-stem-cells
#4
Richard W Naylor, Charles N J McGhee, Chad A Cowan, Alan J Davidson, Teresa M Holm, Trevor Sherwin
Corneal diseases such as keratoconus represent a relatively common disorder in the human population. However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. Cell based therapies may offer an alternative approach given that the eye is amenable to such treatments and corneal diseases like keratoconus have been associated specifically with the death of corneal keratocytes. The ability to generate corneal keratocytes in vitro may enable a cell-based therapy to treat patients with keratoconus...
2016: PloS One
https://www.readbyqxmd.com/read/27783156/arginase-activity-urea-and-hydroxyproline-concentration-are-reduced-in-keratoconus-keratocytes
#5
Tanja Stachon, Krasimir Kolev, Zsuzsa Flaskó, Berthold Seitz, Achim Langenbucher, Nóra Szentmáry
PURPOSE: Keratoconus (KC) is a disease characterized by thinning and deformation of the cornea, but its etiology remains unknown. Seventy percent of the corneal stroma consists of collagen, which is composed of three intertwined polypeptide chains with glycine-hydroxyproline-proline repeats along their sequence. Arginase is a cytoplasmatic enzyme and catalyzes the conversion of arginine to urea and ornithine, which serves as a precursor for the endogenous synthesis of proline and hydroxyproline...
October 25, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27781955/mmp14-regulates-vegfr3-expression-on-corneal-epithelial-cells
#6
Robert Jin-Hong Chang, Dimitri T Azar, Kyu-Yeon Han
Vascular endothelial growth factor receptor 3 (VEGFR3) regulates the growth and differentiation of blood and lymphatic vessels. To determine whether matrix metalloproteinase 14 (MMP14) modulates VEGFR3 expression in the corneal epithelium to influence the avascularity of the cornea, VEGFR3 expression was compared between wild-type and MMP14-deficient (MMP14 Δexon4) corneal epithelial cells. Western blot analysis showed that VEGFR3 protein expression was higher on MMP14 Δexon4 corneal epithelial cells than on wild-type cells, and quantitative RT-PCR analysis showed that VEGFR3 gene expression was highly induced in MMP14 Δexon4 corneal epithelial cells but not in wild-type corneal epithelial cells or wild-type and MMP14 Δexon4 corneal keratocytes...
October 24, 2016: Protein and Peptide Letters
https://www.readbyqxmd.com/read/27770837/effect-of-riboflavin-uva-collagen-cross-linking-on-central-cornea-limbus-and-intraocular-pressure-experimental-study-in-rabbit-eyes
#7
Vassilios Kozobolis, Maria Gkika, Haris Sideroudi, Efthymia Tsaragli, Stylliani Lydataki, Irini Naoumidi, Alexandra Giatromanolaki, Dimitrios Mikropoulos, Miguel Teus, Georgios Labiris
The Purpose of present study was to investigate the effect of riboflavin/ultraviolet-A-induced collagen cross-linking (CXL) on central cornea, limbus and intraocular pressure (IOP). This was an animal experimental study. The right corneas of 10 rabbits were ultraviolet-A irradiated (3 mW/cm2 for 30 minutes) after de-epithelialization and instillation of 0.1% riboflavin / 20% Dextran drops. Left corneas served as controls. Samples were examined histologically one month postoperatively. Before and after treatment, IOP measurements were recorded bilaterally...
2016: Acta Medica (Hradec Králové)
https://www.readbyqxmd.com/read/27760648/-study-on-the-evaluation-of-pterygium-activity-with-in-vivo-confocal-microscopy
#8
Q F Liang, C Gao, H Liang, B Li, X H Du, Antoine Labbe
Objective: To observe the characteristics of pterygium with in vivo confocal microscopy (IVCM) and understand pterygium activity with the density of inflammatory cells, formation of new blood vessels, and the number of activated keratocytes within the stroma. Methods: A prospective case-controlled study. Thirty-six pterygia from 28 patients were analyzed in this study. A pterygium activity score was obtained by summing four scores of ocular discomfort, pterygium hyperemia, keratitis, and the presence of Fuchs patches...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27749452/comparative-confocal-and-histopathological-study-of-corneal-changes-in-multiple-myeloma
#9
Antonio Micali, Anna M Roszkowska, Elisa I Postorino, Laura Rania, Emanuela Aragona, Edward Wylegala, Anna Nowinska, Antonio Ieni, Sebastiano Calimeri, Antonina Pisani, Pasquale Aragona, Domenico Puzzolo
PURPOSE: Corneal opacities rarely occur in multiple myeloma (MM). Our study correlates the findings of in vivo confocal microscopy (IVCM), a useful diagnostic tool, with histopathological features of corneal opacities appearing in a patient with MM. METHODS: Case report. RESULTS: A 53-year-old man developed corneal opacities in both eyes, more pronounced in the left eye. After IVCM examination, he underwent penetrating keratoplasty in the left eye, and the button was processed for light and electron microscopy and immunohistochemistry...
January 2017: Cornea
https://www.readbyqxmd.com/read/27732879/temporal-and-spatial-analysis-of-stromal-cell-and-extracellular-matrix-patterning-following-lamellar-keratectomy
#10
Pouriska B Kivanany, Kyle C Grose, W Matthew Petroll
Extracellular matrix (ECM) supplies both physical and chemical signals to keratocytes which can impact their differentiation to fibroblasts and/or myofibroblasts. It also provides a substrate through which they migrate during wound repair. We have previously shown that following transcorneal freeze injury (FI), migrating corneal fibroblasts align parallel to the stromal lamellae during wound repopulation. In this study, we compare cell and ECM patterning both within and on top of the stroma at different time points following lamellar keratectomy (LK) in the rabbit...
December 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27727144/effect-of-substrate-elasticity-on-macroscopic-parameters-of-fish-keratocyte-migration
#11
Daniel Dziob, Tomasz Kołodziej, Justyna Nowak, Piotr Cyzio, Joanna Raczkowska, Jadwiga Laska, Zenon Rajfur
Cell migration is an important biological phenomenon which depends on a number of internal and external factors. One of such factors can be the mechanical properties of the environment which can have an impact on the cell's regulatory pathways through so-called mechanotransduction. Ultimately, these properties can also influence the process of cell migration. The goal of this work is to investigate how substrate stiffness (elasticity) changes basic migration parameters of migrating cells. Fish keratocytes migrating on polyacrylamide hydrogels have been used as a model of fast migrating cells...
October 11, 2016: Physical Biology
https://www.readbyqxmd.com/read/27710923/in-vitro-characterization-of-electrochemically-compacted-collagen-matrices-for-corneal-applications
#12
Vipuil Kishore, Ranjani Iyer, Athela Frandsen, Thuy-Uyen Nguyen
Loss of vision due to corneal disease is a significant problem worldwide. Transplantation of donor corneas is a viable treatment option but limitations such as short supply and immune-related complications call for alternative options for the treatment of corneal disease. A tissue engineering-based approach using a collagen scaffold is a promising alternative to develop a bioengineered cornea that mimics the functionality of native cornea. In this study, an electrochemical compaction method was employed to synthesize highly dense and transparent collagen matrices...
October 6, 2016: Biomedical Materials
https://www.readbyqxmd.com/read/27689082/efficacy-and-safety-of-lasik-combined-with-accelerated-corneal-collagen-cross-linking-for-myopia-six-month-study
#13
Ying Wu, Lei Tian, Li-Qiang Wang, Yi-Fei Huang
This was a prospective controlled clinical trial. 48 myopia patients (96 eyes) were included in this study. After LASIK, accelerated corneal collagen cross-linking (ACXL) was used for myopia treatment. During 6-month follow-up, the results of LASIK-ACXL treatment were studied and compared to the LASIK-only procedure. The results showed that no statistically significant differences in UDVA, CDVA, MRSE, K mean, pachymetry, or ECD were found between the two groups at the visit after 6 months of follow-up (all P > 0...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27687492/single-cell-analysis-reveals-igf-1-potentiation-of-inhibition-of-the-tgf-%C3%AE-smad-pathway-of-fibrosis-in-human-keratocytes-in-vitro
#14
Tomislav Sarenac, Martin Trapecar, Lidija Gradisnik, Marjan Slak Rupnik, Dusica Pahor
Corneal wound healing is often affected by TGF-β-mediated fibrosis and scar formation. Guided fibrosis with IGF-1 and antifibrotic substances might maintain corneal transparency. Primary human corneal keratocytes under serum-free conditions were used as a model of corneal stromal wounding, with markers of corneal fibrosis and opacity studied under TGF-β2 stimulation. Single-cell imaging flow cytometry was used to determine nuclearization of Smad3, and intracellular fluorescence intensity of Smad7 and the corneal crystallin aldehyde dehydrogenase 3A1...
September 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27685949/corneal-keratocyte-transition-to-mesenchymal-stem-cell-phenotype-and-reversal-using-serum-free-medium-supplemented-with-fgf-2-tgf-%C3%A3-3-and-retinoic-acid
#15
Laura E Sidney, Andrew Hopkinson
Keratocytes of the corneal limbal stroma can derive populations of mesenchymal stem cells (MSC) when expanded in vitro. However, once a corneal MSC (cMSC) phenotype is achieved, regaining the keratocyte phenotype can be challenging, and there is no standardised differentiation medium. Here, we investigated the transition of keratocytes to cMSC and compared different supplements in their ability to return cMSC to a keratocyte phenotype. Immunofluorescence and RT-qPCR demonstrated in vivo keratocyte expression of ALDH3A1, CD34 and keratocan, but not any of the typical MSC markers (CD73, CD90, CD105)...
September 29, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27678055/persistence-of-fan-shaped-keratocytes-is-a-matrix-rigidity-dependent-mechanism-that-requires-%C3%AE-5%C3%AE-1-integrin-engagement
#16
Maryam Riaz, Marie Versaevel, Danahe Mohammed, Karine Glinel, Sylvain Gabriele
Despite the importance of matrix rigidity on cell functions, many aspects of the mechanosensing process in highly migratory cells remain elusive. Here, we studied the migration of highly motile keratocytes on culture substrates with similar biochemical properties and rigidities spanning the range between soft tissues (~kPa) and stiff culture substrates (~GPa). We show that morphology, polarization and persistence of motile keratocytes are regulated by the matrix stiffness over seven orders of magnitude, without changing the cell spreading area...
September 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27661072/regulatory-mechanism-of-collagen-degradation-by-keratocytes-and-corneal-inflammation-the-role-of-urokinase-type-plasminogen-activator
#17
Koji Sugioka, Hiroshi Mishima, Aya Kodama, Motoki Itahashi, Masahiko Fukuda, Yoshikazu Shimomura
Keratocytes, corneal resident cells in the corneal stroma, exist between collagen lamellae and maintain the corneal stromal structure. When the corneal stroma is damaged, keratocytes are transformed to myofibroblasts to aid corneal wound healing by phagocytizing debris. Keratocytes and extracellular collagen influence each other because keratocytes cultured in a 3D collagen gel undergo morphological changes and keratocytes produce metalloproteases that degrade extracellular collagen. IL-1 and plasminogen are critical mediators for collagen degradation...
November 2016: Cornea
https://www.readbyqxmd.com/read/27590038/new-histopathologic-and-ultrastructural-findings-in-reis-b%C3%A3-cklers-corneal-dystrophy-caused-by-the-arg124leu-mutation-of-tgfbi-gene
#18
Wen-Ya Qiu, Li-Bin Zheng, Fei Pan, Bei-Bei Wang, Yu-Feng Yao
BACKGROUND: Reis-Bücklers corneal dystrophy (RBCD) was consistently reported as a corneal dystrophy only affected Bowman's layer and superficial corneal stroma, and superficial keratectomy was a recommendation surgery for treatment in literatures. The study reported new histopathological and ultrastructural findings in RBCD caused by the Arg124Leu mutation of transforming growth factor induced (TGFBI) gene in a four-generation Chinese pedigree. METHODS: Subjects including eight patients and seven unaffected family members received slit-lamp biomicroscopy and photography...
2016: BMC Ophthalmology
https://www.readbyqxmd.com/read/27570987/evaluation-of-the-in-vitro-ocular-toxicity-of-the-fortified-antibiotic-eye-drops-prepared-at-the-hospital-pharmacy-departments
#19
Anxo Fernández-Ferreiro, Miguel González-Barcia, María Gil-Martínez, María Santiago Varela, María Pardo, José Blanco-Méndez, Antonio Piñeiro-Ces, María Jesús Lamas Díaz, Francisco J Otero-Espinar
The use of parenteral antibiotic eye drop formulations with non-marketed compositions or concentrations, commonly called fortified antibiotic eye drops, is a common practice in Ophthalmology in the hospital setting. The aim of this study was to evaluate the in vitro ocular toxicity of the main fortified antibiotic eye drops prepared in the Hospital Pharmacy Departments. We have conducted an in vitro experimental study in order to test the toxicity of gentamicin, amikacin, cefazolin, ceftazidime, vancomycin, colistimethate sodium and imipenem-cilastatin eye drops; their cytotoxicity and acute tissue irritation have been evaluated...
September 2016: Farmacia Hospitalaria
https://www.readbyqxmd.com/read/27567559/prgf-exerts-more-potent-proliferative-and-anti-inflammatory-effects-than-autologous-serum-on-a-cell-culture-inflammatory-model
#20
E Anitua, F Muruzabal, M de la Fuente, A Riestra, J Merayo-Lloves, G Orive
Ocular graft versus host disease (oGVHD) is part of a systemic inflammatory disease that usually affects ocular surface tissues manifesting as a dry eye syndrome. Current treatments provide unsatisfactory results. Blood-derived products, like plasma rich in growth factors (PRGF) emerge as a potential therapy for this disease. The purpose of this study was to evaluate the tissue regeneration and anti-inflammatory capability of PRGF, an autologous platelet enriched plasma eye-drop, compared to autologous serum (AS) obtained from oGVHD patients on ocular surface cells cultured in a pro-inflammatory environment...
October 2016: Experimental Eye Research
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