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https://www.readbyqxmd.com/read/28428541/tissue-engineered-cornea-constructed-with-compressed-collagen-and-laser-perforated-electrospun-mat
#1
Bin Kong, Wei Sun, Guoshi Chen, Song Tang, Ming Li, Zengwu Shao, Shengli Mi
While Plastic Compressed (PC) collagen technique is often used to fabricate bioengineered constructs, PC collagen gels are too weak to be sutured or conveniently handled for clinical applications. To overcome this limitation, electrospun poly (lactic-co-glycolide) (PLGA) mats, which have excellent biocompatibility and mechanical properties, were combined with PC collagen to fabricate sandwich-like hybrid constructs. By laser-perforating holes with different sizes and spacings in the electrospun mats to regulate the mechanical properties and light transmittance of the hybrid constructs, we produced hybrid constructs with properties very suitable to apply in corneal tissue engineering...
April 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28410358/reactivation-of-herpes-zoster-keratitis-with-corneal-perforation-after-zoster-vaccination
#2
Andre Jastrzebski, Seymour Brownstein, Setareh Ziai, Solin Saleh, Kay Lam, W Bruce Jackson
PURPOSE: We present a case of reactivated herpes zoster keratouveitis of 6 years duration with corneal perforation requiring penetrating keratoplasty shortly after inoculation with herpes zoster vaccine (Zostavax, Merck, Quebec, Canada). METHODS: Retrospective case report. RESULTS: A 67-year-old woman with a 5-year history of recurrent unilateral herpes zoster keratouveitis in her right eye presented with another recurrence 2 weeks after Zostavax vaccination...
April 12, 2017: Cornea
https://www.readbyqxmd.com/read/28393020/effects-of-exogenous-recombinant-human-bone-morphogenic-protein-7-on-the-corneal-epithelial-mesenchymal-transition-and-fibrosis
#3
Jin Kwon Chung, Shin Ae Park, Hee Sun Hwang, Kwang Sung Kim, Yang Je Cho, Yong Sung You, Young Sik Kim, Ju Woong Jang, Sung Jin Lee
AIM: To evaluate the effect of exogenous recombinant human bone morphogenic protein-7 (rhBMP-7) on transforming growth factor-β (TGF-β)-induced epithelial mesenchymal cell transition (EMT) and assessed its antifibrotic effect via topical application. METHODS: The cytotoxic effect of rhBMP-7 was evaluated and the EMT of human corneal epithelial cells (HECEs) was induced by TGF-β. HECEs were then cultured in the presence of rhBMP-7 and/or hyaluronic acid (HA). EMT markers, fibronectin, E-cadherin, α-smooth muscle actin (α-SMA), and matrix metaloproteinase-9 (MMP-9), were evaluated...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28384032/in-vitro-evaluation-of-the-ophthalmic-toxicity-profile-of-chlorhexidine-and-propamidine-isethionate-eye-drops
#4
Anxo Fernández-Ferreiro, María Santiago-Varela, María Gil-Martínez, Miguel González-Barcia, Andrea Luaces-Rodríguez, Victoria Díaz-Tome, María Pardo, José Blanco Méndez, Antonio Piñeiro-Ces, María Teresa Rodríguez-Ares, Maria Jesus Lamas, Francisco J Otero-Espinar
PURPOSE: Acanthamoeba keratitis causes frequent epithelial lesions that fully expose the corneal stroma. The aim of this study was to determine the toxic profile of chlorhexidine and propamidine eye drops. METHODS: We used primary human keratocytes in cell culture in combination with a novel technology that evaluates dynamic real-time cytotoxicity through impedance analysis. Additional studies such as a classic cell viability test (WST-1(®)), a bovine corneal opacity and permeability assay, and an irritation eye study (Hen's Egg Test [HET]) have been made...
April 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28366375/measuring-the-depth-of-crosslinking-demarcation-line-in%C3%A2-vivo-comparison-of-methods-and-devices
#5
Andreas Thorsrud, Gunhild F Sandvik, Anne M Hagem, Liv Drolsum
PURPOSE: To assess the interrelationship of different methods of measuring the demarcation line depth after corneal collagen crosslinking (CXL). SETTING: University eye clinic, Oslo, Norway. DESIGN: Prospective case series. METHODS: Eyes having CXL for progressive keratoconus were evaluated 1 month after CXL by in vitro confocal microscopy (IVCM), optical coherence tomography (OCT), and Scheimpflug imaging. When applying IVCM, the depth of the CXL demarcation line was measured with 2 methods; that is, IVCM keratocyte disappearance and IVCM intensity increase...
February 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28349952/nerve-regeneration-by-human-corneal-stromal-keratocytes-and-stromal-fibroblasts
#6
Gary Hin-Fai Yam, Geraint P Williams, Melina Setiawan, Nur Zahirah Binte M Yusoff, Xiao-Wen Lee, Hla Myint Htoon, Lei Zhou, Matthias Fuest, Jodhbir S Mehta
Laser refractive surgeries reshape corneal stroma to correct refractive errors, but unavoidably affect corneal nerves. Slow nerve regeneration and atypical neurite morphology cause desensitization and neuro-epitheliopathy. Following injury, surviving corneal stromal keratocytes (CSKs) are activated to stromal fibroblasts (SFs). How these two different cell types influence nerve regeneration is elusive. Our study evaluated the neuro-regulatory effects of human SFs versus CSKs derived from the same corneal stroma using an in vitro chick dorsal root ganglion model...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28346441/altering-the-threshold-of-an-excitable-signal-transduction-network-changes-cell-migratory-modes
#7
Yuchuan Miao, Sayak Bhattacharya, Marc Edwards, Huaqing Cai, Takanari Inoue, Pablo A Iglesias, Peter N Devreotes
The diverse migratory modes displayed by different cell types are generally believed to be idiosyncratic. Here we show that the migratory behaviour of Dictyostelium was switched from amoeboid to keratocyte-like and oscillatory modes by synthetically decreasing phosphatidylinositol-4,5-bisphosphate levels or increasing Ras/Rap-related activities. The perturbations at these key nodes of an excitable signal transduction network initiated a causal chain of events: the threshold for network activation was lowered, the speed and range of propagating waves of signal transduction activity increased, actin-driven cellular protrusions expanded and, consequently, the cell migratory mode transitions ensued...
April 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28339457/targeted-aav5-smad7-gene-therapy-inhibits-corneal-scarring-in-vivo
#8
Suneel Gupta, Jason T Rodier, Ajay Sharma, Elizabeth A Giuliano, Prashant R Sinha, Nathan P Hesemann, Arkasubhra Ghosh, Rajiv R Mohan
Corneal scarring is due to aberrant activity of the transforming growth factor β (TGFβ) signaling pathway following traumatic, mechanical, infectious, or surgical injury. Altered TGFβ signaling cascade leads to downstream Smad (Suppressor of mothers against decapentaplegic) protein-mediated signaling events that regulate expression of extracellular matrix and myogenic proteins. These events lead to transdifferentiation of keratocytes into myofibroblasts through fibroblasts and often results in permanent corneal scarring...
2017: PloS One
https://www.readbyqxmd.com/read/28302098/in-vivo-confocal-microscopy-of-pre-descemet-corneal-dystrophy-associated-with-x-linked-ichthyosis-a-case-report
#9
Hui Shi, Xiao-Feng Qi, Tao-Tao Liu, Qian Hao, Xiao-Hong Li, Ling-Ling Liang, Yi-Miao Wang, Zhi-Hua Cui
BACKGROUND: Pre-Descemet corneal dystrophy (PDCD) is characterized by the presence of numerous, tiny, polymorphic opacities immediately anterior to Descemet membrane, which is a rare form of corneal stromal dystrophy and hard to be diagnosed. In vivo confocal microscopy (IVCM) is a useful tool to examine the minimal lesions of the cornea at the cellular level. In this article, we report a rare case of PDCD associated with X-linked ichthyosis and evaluate IVCM findings. CASE PRESENTATION: We present a 34-year-old male Chinese patient with PDCD associated with X-linked ichthyosis...
March 16, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28301610/the-effects-of-substance-p-and-acetylcholine-on-human-tenocyte-proliferation-converge-mechanistically-via-tgf-%C3%AE-1
#10
Gloria Fong, Ludvig J Backman, Håkan Alfredson, Alex Scott, Patrik Danielson
Previous in vitro studies on human tendon cells (tenocytes) have demonstrated that the exogenous administration of substance P (SP) and acetylcholine (ACh) independently result in tenocyte proliferation, which is a prominent feature of tendinosis. Interestingly, the possible link between SP and ACh has not yet been explored in human tenocytes. Recent studies in other cell types demonstrate that both SP and ACh independently upregulate TGF-β1 expression via their respective receptors, the neurokinin 1 receptor (NK-1R) and muscarinic ACh receptors (mAChRs)...
2017: PloS One
https://www.readbyqxmd.com/read/28273315/extracellular-collagen-promotes-interleukin-1%C3%AE-induced-urokinase-type-plasminogen-activator-production-by-human-corneal-fibroblasts
#11
Koji Sugioka, Aya Kodama-Takahashi, Koji Yoshida, Keiichi Aomatsu, Kiyotaka Okada, Teruo Nishida, Yoshikazu Shimomura
Purpose: Keratocytes maintain homeostasis of the corneal stroma through synthesis, secretion, and degradation of collagen fibrils of the extracellular matrix. Given that these cells are essentially embedded in a collagen matrix, keratocyte-collagen interactions may play a key role in regulation of the expression or activation of enzymes responsible for matrix degradation including urokinase-type plasminogen activator (uPA), plasmin, and matrix metalloproteinases (MMPs). We examined the effect of extracellular collagen on the production of uPA by corneal fibroblasts (activated keratocytes) stimulated with the proinflammatory cytokine interleukin-1β (IL-1β)...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#12
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28264133/why-non-contact-tonometry-tests-cannot-evaluate-the-effects-of-corneal-collagen-cross-linking
#13
Ángel Ortillés, José F Rodríguez-Matas, Miguel Á Ariza-Gracia, Gemma Pascual, Begoña Calvo
PURPOSE: To assess the feasibility of characterizing and following up the mechanical behavior of the corneal tissue after corneal cross-linking (CXL) by using a combined mechanical (in vivo indentation and in vitro uniaxial tensile tests) and morphological (immunohisto-chemistry) experimental protocol. METHODS: CXL (3 mW/cm(2); 370 nm) for 20 minutes (total dose 3.6 J/cm(2)) was performed on 12 New Zealand rabbits. The mechanical behavior of the cornea was characterized in small and large strain regimens using an in vivo indentation test with a laboratory device and an in vitro uniaxial tensile test, respectively...
March 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28264131/ex-vivo-study-of-transepithelial-corneal-cross-linking
#14
Andrea Cruzat, Anita N Shukla, Samer N Arafat, Saleh Alageel, Clara Colon, James Chodosh, Joseph B Ciolino
PURPOSE: To perform in vitro assessment of different techniques of transepithelial corneal cross-linking (CXL) and to compare the results to deepithelialized CXL. METHODS: Transepithelial CXL was performed after pre-treatment with or without penetration enhancers (gum cellulose, 0.44% sodium chloride, and 0.01% benzalkonium chloride) for 15 or 60 minutes. Deepithelialized corneas underwent CXL after pretreatment with riboflavin for 15 minutes, according to the Dresden protocol...
March 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28257425/corneal-stromal-stem-cells-reduce-corneal-scarring-by-mediating-neutrophil-infiltration-after-wounding
#15
Andrew J Hertsenberg, Golnar Shojaati, Martha L Funderburgh, Mary M Mann, Yiqin Du, James L Funderburgh
Corneal scarring limits vision for millions of individuals worldwide. Corneal transplantation (keratoplasty) is the standard of care for corneal opacity; however, it bears the risk of graft rejection and infection and is not universally available. Stem cell therapy holds promise as an alternative to keratoplasty. Stem cells from human corneal stroma (CSSC) induce regeneration of transparent corneal tissue in a mouse wound-healing model. In this study we investigated the mechanism by which CSSC prevent deposition of fibrotic tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28253401/netrin-4-mediates-corneal-hemangiogenesis-but-not-lymphangiogenesis-in-the-mouse-model-of-suture-induced-neovascularization
#16
Anna-Karina B Maier, Sabrina Klein, Norbert Kociok, Aline I Riechardt, Enken Gundlach, Nadine Reichhart, Olaf Strauß, Antonia M Joussen
Purpose: Netrin-4, a secreted protein, is found in the basement membrane of blood vessels and acts as a key regulator of angiogenesis. Here we investigated the role of Netrin-4 in the mouse-model of suture-induced corneal hem- and lymphangiogenesis. Methods: Corneal hem- and lymphangiogenesis were induced in Netrin-4-deficient (Ntn4-/-) and wild-type (WT) mice by placing three 11-0 nylon sutures intrastromally. Fourteen days after suturing, the vascularized area was analyzed via corneal flat mount immunohistochemistry...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28251081/effects-of-contact-lens-wearing-on-keratoconus-a-confocal-microscopy-observation
#17
Somnath Ghosh, Haliza A Mutalib, Sharanjeet-Kaur, Rituparna Ghoshal, Shamala Retnasabapathy
AIM: To evaluate the corneal cell morphology of new keratoconus patients wearing two different types of rigid gas-permeable (RGP) contact lenses for 1y. METHODS: Thirty nine eyes of 39 new keratoconus patients were selected and randomly fitted with two types of RGP contact lenses. Group 1 had 21 eyes with regular rigid gas-permeable (RRGP) contact lens and rest 18 eyes were in group 2 with specially designed rigid gas-permeable (SRGP) contact lens. Corneal cell morphology was evaluated using a slit scanning confocal microscope at no-lens wear and after 1y of contact lens wearing...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28247507/development-of-retrocorneal-membrane-following-pig-to-monkey-penetrating-keratoplasty
#18
Whayoung Lee, Alex Mammen, Deepinder K Dhaliwal, Cassandra Long, Yuko Miyagawa, David Ayares, David K C Cooper, Hidetaka Hara
Recent reports of long-term survival after wild-type (WT) pig-to-monkey corneal xenotransplantation are encouraging. We experienced the rapid development of retrocorneal membranes, a rare complication after corneal allotransplantation (although seen in infants and young children). The original specific aim of the study was to determine the factors associated with successful (young) pig corneal transplantation in monkeys. However, when it was obvious that retrocorneal membranes rapidly developed, our aims became to determine the factors involved in its development after both WT and Genetically engineered (GE ) pig corneal xenotransplantation and to investigate the characteristics of the retrocorneal membrane...
January 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28215504/a-case-of-in-vivo-iontophoresis-assisted-corneal-collagen-cross-linking-for-keratoconus-an-immunohistochemical-study
#19
Mirko Manetti, Eleonora Favuzza, Eleonora Sgambati, Rita Mencucci, Mirca Marini
The standard corneal collagen cross-linking (CXL), that includes the removal of corneal epithelium to permit adequate penetration of riboflavin in the stroma, is an established procedure to halting keratoconus progression. However, as epithelial removal may cause postoperative pain and an increased risk of corneal infection, new therapeutic approaches have been proposed. Iontophoresis is a recently developed non-invasive technique which provides the use of electrical current during CXL to enhance transepithelial penetration of riboflavin into the corneal stroma...
February 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28204869/-corneal-cell-therapy-an-overview
#20
REVIEW
M Fuest, G Hin-Fai Yam, G Swee-Lim Peh, P Walter, N Plange, J S Mehta
In recent years, the cultivation and expansion of primary corneal cells has made significant progress. The transplantation of cultured limbal epithelial cells represents a successful and established treatment of the ocular surface. Cultivated corneal endothelial cells are undergoing a clinical trial in Japan. Stromal keratocytes can now be expanded in vitro. A wide range of stem cell sources is being tested in vitro and animal models for their possible application in corneal cell therapy. This article gives an overview of recent advancements and prevailing limitations for the use of different cell sources in the therapy of corneal disease...
February 15, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
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