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https://www.readbyqxmd.com/read/28302098/in-vivo-confocal-microscopy-of-pre-descemet-corneal-dystrophy-associated-with-x-linked-ichthyosis-a-case-report
#1
Hui Shi, Xiao-Feng Qi, Tao-Tao Liu, Qian Hao, Xiao-Hong Li, Ling-Ling Liang, Yi-Miao Wang, Zhi-Hua Cui
BACKGROUND: Pre-Descemet corneal dystrophy (PDCD) is characterized by the presence of numerous, tiny, polymorphic opacities immediately anterior to Descemet membrane, which is a rare form of corneal stromal dystrophy and hard to be diagnosed. In vivo confocal microscopy (IVCM) is a useful tool to examine the minimal lesions of the cornea at the cellular level. In this article, we report a rare case of PDCD associated with X-linked ichthyosis and evaluate IVCM findings. CASE PRESENTATION: We present a 34-year-old male Chinese patient with PDCD associated with X-linked ichthyosis...
March 16, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28301610/the-effects-of-substance-p-and-acetylcholine-on-human-tenocyte-proliferation-converge-mechanistically-via-tgf-%C3%AE-1
#2
Gloria Fong, Ludvig J Backman, Håkan Alfredson, Alex Scott, Patrik Danielson
Previous in vitro studies on human tendon cells (tenocytes) have demonstrated that the exogenous administration of substance P (SP) and acetylcholine (ACh) independently result in tenocyte proliferation, which is a prominent feature of tendinosis. Interestingly, the possible link between SP and ACh has not yet been explored in human tenocytes. Recent studies in other cell types demonstrate that both SP and ACh independently upregulate TGF-β1 expression via their respective receptors, the neurokinin 1 receptor (NK-1R) and muscarinic ACh receptors (mAChRs)...
2017: PloS One
https://www.readbyqxmd.com/read/28273315/extracellular-collagen-promotes-interleukin-1%C3%AE-induced-urokinase-type-plasminogen-activator-production-by-human-corneal-fibroblasts
#3
Koji Sugioka, Aya Kodama-Takahashi, Koji Yoshida, Keiichi Aomatsu, Kiyotaka Okada, Teruo Nishida, Yoshikazu Shimomura
Purpose: Keratocytes maintain homeostasis of the corneal stroma through synthesis, secretion, and degradation of collagen fibrils of the extracellular matrix. Given that these cells are essentially embedded in a collagen matrix, keratocyte-collagen interactions may play a key role in regulation of the expression or activation of enzymes responsible for matrix degradation including urokinase-type plasminogen activator (uPA), plasmin, and matrix metalloproteinases (MMPs). We examined the effect of extracellular collagen on the production of uPA by corneal fibroblasts (activated keratocytes) stimulated with the proinflammatory cytokine interleukin-1β (IL-1β)...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#4
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28264133/why-non-contact-tonometry-tests-cannot-evaluate-the-effects-of-corneal-collagen-cross-linking
#5
Ángel Ortillés, José F Rodríguez-Matas, Miguel Á Ariza-Gracia, Gemma Pascual, Begoña Calvo
PURPOSE: To assess the feasibility of characterizing and following up the mechanical behavior of the corneal tissue after corneal cross-linking (CXL) by using a combined mechanical (in vivo indentation and in vitro uniaxial tensile tests) and morphological (immunohisto-chemistry) experimental protocol. METHODS: CXL (3 mW/cm(2); 370 nm) for 20 minutes (total dose 3.6 J/cm(2)) was performed on 12 New Zealand rabbits. The mechanical behavior of the cornea was characterized in small and large strain regimens using an in vivo indentation test with a laboratory device and an in vitro uniaxial tensile test, respectively...
March 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28264131/ex-vivo-study-of-transepithelial-corneal-cross-linking
#6
Andrea Cruzat, Anita N Shukla, Samer N Arafat, Saleh Alageel, Clara Colon, James Chodosh, Joseph B Ciolino
PURPOSE: To perform in vitro assessment of different techniques of transepithelial corneal cross-linking (CXL) and to compare the results to deepithelialized CXL. METHODS: Transepithelial CXL was performed after pre-treatment with or without penetration enhancers (gum cellulose, 0.44% sodium chloride, and 0.01% benzalkonium chloride) for 15 or 60 minutes. Deepithelialized corneas underwent CXL after pretreatment with riboflavin for 15 minutes, according to the Dresden protocol...
March 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/28257425/corneal-stromal-stem-cells-reduce-corneal-scarring-by-mediating-neutrophil-infiltration-after-wounding
#7
Andrew J Hertsenberg, Golnar Shojaati, Martha L Funderburgh, Mary M Mann, Yiqin Du, James L Funderburgh
Corneal scarring limits vision for millions of individuals worldwide. Corneal transplantation (keratoplasty) is the standard of care for corneal opacity; however, it bears the risk of graft rejection and infection and is not universally available. Stem cell therapy holds promise as an alternative to keratoplasty. Stem cells from human corneal stroma (CSSC) induce regeneration of transparent corneal tissue in a mouse wound-healing model. In this study we investigated the mechanism by which CSSC prevent deposition of fibrotic tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28253401/netrin-4-mediates-corneal-hemangiogenesis-but-not-lymphangiogenesis-in-the-mouse-model-of-suture-induced-neovascularization
#8
Anna-Karina B Maier, Sabrina Klein, Norbert Kociok, Aline I Riechardt, Enken Gundlach, Nadine Reichhart, Olaf Strauß, Antonia M Joussen
Purpose: Netrin-4, a secreted protein, is found in the basement membrane of blood vessels and acts as a key regulator of angiogenesis. Here we investigated the role of Netrin-4 in the mouse-model of suture-induced corneal hem- and lymphangiogenesis. Methods: Corneal hem- and lymphangiogenesis were induced in Netrin-4-deficient (Ntn4-/-) and wild-type (WT) mice by placing three 11-0 nylon sutures intrastromally. Fourteen days after suturing, the vascularized area was analyzed via corneal flat mount immunohistochemistry...
March 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28251081/effects-of-contact-lens-wearing-on-keratoconus-a-confocal-microscopy-observation
#9
Somnath Ghosh, Haliza A Mutalib, Sharanjeet-Kaur, Rituparna Ghoshal, Shamala Retnasabapathy
AIM: To evaluate the corneal cell morphology of new keratoconus patients wearing two different types of rigid gas-permeable (RGP) contact lenses for 1y. METHODS: Thirty nine eyes of 39 new keratoconus patients were selected and randomly fitted with two types of RGP contact lenses. Group 1 had 21 eyes with regular rigid gas-permeable (RRGP) contact lens and rest 18 eyes were in group 2 with specially designed rigid gas-permeable (SRGP) contact lens. Corneal cell morphology was evaluated using a slit scanning confocal microscope at no-lens wear and after 1y of contact lens wearing...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28247507/development-of-retrocorneal-membrane-following-pig-to-monkey-penetrating-keratoplasty
#10
Whayoung Lee, Alex Mammen, Deepinder K Dhaliwal, Cassandra Long, Yuko Miyagawa, David Ayares, David K C Cooper, Hidetaka Hara
Recent reports of long-term survival after wild-type (WT) pig-to-monkey corneal xenotransplantation are encouraging. We experienced the rapid development of retrocorneal membranes, a rare complication after corneal allotransplantation (although seen in infants and young children). The original specific aim of the study was to determine the factors associated with successful (young) pig corneal transplantation in monkeys. However, when it was obvious that retrocorneal membranes rapidly developed, our aims became to determine the factors involved in its development after both WT and Genetically engineered (GE ) pig corneal xenotransplantation and to investigate the characteristics of the retrocorneal membrane...
January 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28215504/a-case-of-in-vivo-iontophoresis-assisted-corneal-collagen-cross-linking-for-keratoconus-an-immunohistochemical-study
#11
Mirko Manetti, Eleonora Favuzza, Eleonora Sgambati, Rita Mencucci, Mirca Marini
The standard corneal collagen cross-linking (CXL), that includes the removal of corneal epithelium to permit adequate penetration of riboflavin in the stroma, is an established procedure to halting keratoconus progression. However, as epithelial removal may cause postoperative pain and an increased risk of corneal infection, new therapeutic approaches have been proposed. Iontophoresis is a recently developed non-invasive technique which provides the use of electrical current during CXL to enhance transepithelial penetration of riboflavin into the corneal stroma...
February 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28204869/-corneal-cell-therapy-an-overview
#12
REVIEW
M Fuest, G Hin-Fai Yam, G Swee-Lim Peh, P Walter, N Plange, J S Mehta
In recent years, the cultivation and expansion of primary corneal cells has made significant progress. The transplantation of cultured limbal epithelial cells represents a successful and established treatment of the ocular surface. Cultivated corneal endothelial cells are undergoing a clinical trial in Japan. Stromal keratocytes can now be expanded in vitro. A wide range of stem cell sources is being tested in vitro and animal models for their possible application in corneal cell therapy. This article gives an overview of recent advancements and prevailing limitations for the use of different cell sources in the therapy of corneal disease...
February 15, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28165027/potential-role-of-corneal-epithelial-cell-derived-exosomes-in-corneal-wound-healing-and-neovascularization
#13
Kyu-Yeon Han, Jennifer A Tran, Jin-Hong Chang, Dimitri T Azar, James D Zieske
Specific factors from the corneal epithelium underlying the stimulation of stromal fibrosis and myofibroblast formation in corneal wound healing have not been fully elucidated. Given that exosomes are known to transfer bioactive molecules among cells and play crucial roles in wound healing, angiogenesis, and cancer, we hypothesized that corneal epithelial cell-derived exosomes may gain access to the underlying stromal fibroblasts upon disruption of the epithelial basement membrane and that they induce signaling events essential for corneal wound healing...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28157225/corneal-confocal-scanning-laser-microscopy-in-patients-with-dry-eye-disease-treated-with-topical-cyclosporine
#14
B Iaccheri, G Torroni, C Cagini, T Fiore, A Cerquaglia, M Lupidi, S Cillino, H S Dua
PurposeTo investigate the effect of cyclosporine on corneal ultrastructure and on major signs and symptoms of patients with dry eye disease.Patients and methodsIn this prospective cohort study, patients with dry eye disease were treated with a drop of cyclosporine 0.05% twice daily. Clinical evaluation was carried out at baseline and at months 1, 3, and 6. All patients completed the Ocular Surface Disease Index (OSDI) questionnaire, and tear film break-up time (BUT), fluorescein and lissamine green staining, and Schirmer test were carried out...
February 3, 2017: Eye
https://www.readbyqxmd.com/read/28150361/cornea-and-its-adaptation-to-environment-and-accommodation-function-in-veiled-chameleon-chamaeleo-calyptratus-ultrastructure-and-3d-transmission-electron-tomography
#15
Saeed Akhtar, Adnan A Khan, Hussain A Albuhayzah, Turki M Almubrad
We investigate the ultrastructural features and 3D electron tomography of chameleon (Chamaeleon calyptratus) which is a native of desert environments of Saudi Arabia. The corneas of the chameleon were fixed in 2.5% glutaraldehyde containing cuprolinic blue in sodium acetate buffer for electron microscopy and tomography, and observed under a JEOL 1400 transmission electron microscope. The thin cornea (21.92 μm) contained 28-30 collagen fibril lamellae. The middle stromal lamellae (from 13 to 19) contained keratocytes with a long cell process and filled with granular material...
February 1, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/28138416/retinoic-acid-enhances-the-differentiation-of-adipose-derived-stem-cells-to-keratocytes-in-vitro
#16
Amy P Lynch, Mark Ahearne
PURPOSE: All-trans retinoic acid (RA) supplementation was investigated as a method of enhancing the differentiation of human adipose-derived stem cells (ASCs) to corneal keratocytes in vitro, in combination with a chemically defined serum-free medium. METHODS: Adipose-derived stem cells were cultured in monolayer and supplemented with 0.1, 1, or 10 μM RA for 14 days. The effects of RA on cell proliferation, migration, and extracellular matrix (ECM) accumulation were evaluated...
January 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28133954/new-parameters-in-assessment-of-human-donor-corneal-stroma
#17
Marie Borderie, Kate Grieve, Kristina Irsch, Djida Ghoubay, Cristina Georgeon, Celine De Sousa, Laurent Laroche, Vincent M Borderie
PURPOSE: To provide quantitative parameters for assessment of human donor corneal stroma by imaging stromal features of diseased and normal human corneas with full-field optical coherence microscopy (FFOCM), using confocal microscopy (CM) and histology as reference techniques. METHODS: Bowman's layer (BL) thickness and keratocyte density were assessed ex vivo in 23 human donor corneas and 27 human pathological corneas (keratoconus and other corneal disorders) with FFOCM, CM and histology...
January 30, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28118663/the-effects-of-nonporous-silica-nanoparticles-on-cultured-human-keratocytes
#18
Bora Yim, Joo-Hee Park, Hyejoong Jeong, Jinkee Hong, Young-Joo Shin, Roy S Chuck, Choul Yong Park
Purpose: Silica nanoparticles (SiNPs) are promising carriers for ophthalmic drug delivery. In this study, we investigated the effect of various sizes of nonporous SiNPs on cultured human keratocytes. Methods: Three different sizes of SiNPs (50, 100, and 150 nm) were manufactured. Primarily cultured human keratocytes were exposed to different concentrations (0, 25, 50, and 100 μg/mL) of three sizes of SiNPs for up to 72 hours. Intracellular reactive oxygen species (ROS) generation, cellular viability, lactate dehydrogenase (LDH) assay, autophagy, vimentin expression, and mammalian target of rapamycin (mTOR) pathway activation were evaluated...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28101157/functional-analysis-of-keratinocyte-and-fibroblast-gene-expression-in-skin-and-keloid-scar-tissue-based-on-deviation-analysis-of-dynamic-capabilities
#19
Mingming Li, Lei Wu
The aim of the present study was to select key genes that are associated with fibroblasts and keratinocytes during keloid scar progression and development. The gene expression profile of GSE44270, which includes 32 samples, was downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) in case samples compared with control samples were screened using the Limma R package followed by hierarchical clustering analysis. Protein-protein interaction (PPI) networks of the total selected DEGs were constructed using Cytoscape...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28099503/3d-functional-corneal-stromal-tissue-equivalent-based-on-corneal-stromal-stem-cells-and-multi-layered-silk-film-architecture
#20
Chiara E Ghezzi, Benedetto Marelli, Fiorenzo G Omenetto, James L Funderburgh, David L Kaplan
The worldwide need for human cornea equivalents continues to grow. Few clinical options are limited to allogenic and synthetic material replacements. We hypothesized that tissue engineered human cornea systems based on mechanically robust, patterned, porous, thin, optically clear silk protein films, in combination with human corneal stromal stem cells (hCSSCs), would generate 3D functional corneal stroma tissue equivalents, in comparison to previously developed 2D approaches. Silk film contact guidance was used to control the alignment and distribution of hCSSCs on RGD-treated single porous silk films, which were then stacked in an orthogonally, multi-layered architecture and cultured for 9 weeks...
2017: PloS One
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