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https://www.readbyqxmd.com/read/28635135/the-gene-expression-landscape-of-pine-seedling-tissues
#1
Rafael A Cañas, Zhen Li, M Belén Pascual, Vanessa Castro-Rodríguez, Concepción Ávila, Lieven Sterck, Yves Van de Peer, Francisco M Cánovas
Conifers dominate vast regions of the Northern hemisphere. They are the main source of raw materials for timber industry as well as a wide range of biomaterials. Despite their inherent difficulties as experimental models for classical plant biology research, the technological advances in genomics research are enabling fundamental studies on these plants. The use of laser capture microdissection followed by transcriptomic analysis is a powerful tool for unravelling the molecular and functional organization of conifer tissues and specialized cells...
June 21, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28633431/differential-effects-of-alcohol-on-memory-performance-in-adolescent-men-and-women-with-a-binge-drinking-history
#2
Concepción Vinader-Caerols, Andrew Talk, Adriana Montañés, Aránzazu Duque, Santiago Monleón
Aims: Binge drinking (BD) is characterized by intermittent consumption of large quantities of alcohol in short periods. This pattern of drinking is prevalent among adolescents, and has been associated with undermined learning and memory ability. This study investigates the relationships between a history of BD and the effects of acute exposure to alcohol on learning and memory performance in adolescent men and women. Methods: A high, acute dose of alcohol or control refreshment was administered to a sample of 172 adolescent undergraduate students, some of which had a history of BD and others of which had refrained from alcohol consumption...
June 17, 2017: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/28632402/3-benzyloxy-1-5-18-f-fluoropentyl-5-nitro-1h-indazole-a-pet-radiotracer-to-measure-acetylcholinesterase-in-brain
#3
Soledad Fernández, Javier Giglio, Ana Laura Reyes, Andrés Damián, Concepción Pérez, Daniel I Pérez, Mercedes González, Patricia Oliver, Ana Rey, Henry Engler, Hugo Cerecetto
AIM: Noninvasive studies of the acetylcholinesterase (AChE) level in Alzheimer's disease (AD) patients can contribute to a better understanding of the disease and its therapeutic. We propose 3-(benzyloxy)-1-(5-[(18)F]fluoropentyl)-5-nitro-1H-indazole, [(18)F]-IND1, structurally related to the AChE-inhibitor CP126,998, as a new positron emission tomography-radiotracer. EXPERIMENTAL: Radiosynthesis, with 18F, stability, lipophilicity and protein binding of [18F]-IND1 were studied...
June 20, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28632158/on-the-application-of-image-processing-methods-for-bubble-recognition-to-the-study-of-subcooled-flow-boiling-of-water-in-rectangular-channels
#4
Concepción Paz, Marcos Conde, Jacobo Porteiro, Miguel Concheiro
This work introduces the use of machine vision in the massive bubble recognition process, which supports the validation of boiling models involving bubble dynamics, as well as nucleation frequency, active site density and size of the bubbles. The two algorithms presented are meant to be run employing quite standard images of the bubbling process, recorded in general-purpose boiling facilities. The recognition routines are easily adaptable to other facilities if a minimum number of precautions are taken in the setup and in the treatment of the information...
June 20, 2017: Sensors
https://www.readbyqxmd.com/read/28628470/vascularity-of-nail-bed-by-ultrasound-to-discriminate-psoriasis-psoriatic-arthritis-and-healthy-controls
#5
Sibel Zehra Aydin, Concepción Castillo-Gallego, Zoe R Ash, Helena Marzo-Ortega, Richard Wakefield, Dennis McGonagle
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June 16, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28627565/a-stable-gold-i-enyne-species-obtained-by-alkyne-carboauration-in-a-complex-rearrangement
#6
Jéssica Cámara, M Carmen Blanco, Antonio Laguna, Panče Naumov, M Concepción Gimeno
An unprecedented tetranuclear gold derivative with unusual gold-enyne moieties is prepared by a mild and neat rearrangement of a dinuclear gold complex with a bridging bis(diphenylphosphino)alkyne and terminal alkynyl ligands. The complex originates as a consequence of an intramolecular addition of the AuC[triple bond, length as m-dash]CTol fragment to the internal diphosphine triple bond Ph2PC[triple bond, length as m-dash]CPPh2. The crystal structure of the tetranuclear complex shows a dinuclear metallacycle with a very short AuAu bond interaction and bridging phosphino-enyne ligands...
June 19, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28627202/infrapeso-materno-y-resultados-perinatales-estudio-de-cohortes-retrospectivo
#7
Ángel Vilar Sánchez, Juan Jesús Fernández Alba, María Del Carmen González Macías, María Del Carmen Paublete Herrera, Concepción Carnicer Fuentes, Florentino Carral San Laureano, Rafael Torrejón Cardoso, Luis Javier Moreno Corral
Introducción: algunos estudios han asociado el infrapeso materno con resultados perinatales adversos tales como aborto espontáneo, desprendimiento placentario, feto pequeño para edad gestacional, crecimiento intrauterino retardado y parto pretérmino.Objetivos: determinar si el infrapeso materno al inicio de la gestación influye sobre la forma de inicio y vía del parto, peso al nacer, índice de Apgar al minuto 5 y edad gestacional en el momento del parto.Métodos: estudio de cohortes retrospectivo en gestantes adscritas al Hospital Universitario de Puerto Real...
June 5, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/28619364/outcome-of-arterial-ischemic-stroke-in-children-with-heart-disease
#8
María Vázquez López, Pedro de Castro de Castro, Estibaliz Barredo Valderrama, Ma Concepción Miranda Herrero, Nuria Gil Villanueva, Andrés J Alcaraz Romero, Samuel I Pascual Pascual
Arterial ischemic strokes (AIS) are rare in childhood. Congenital and acquired heart diseases are one of the most important risk factors of AIS in children. OBJECTIVE: Study the outcome of children with heart disease that have suffered AIS and the factors that influence on prognosis. PATIENTS AND METHODS: We evaluated all children with heart disease who had suffered AIS between 2000 and 2014 in our hospital. RESULTS: Seventy-four children with heart disease suffered an arterial ischemic stroke...
June 1, 2017: European Journal of Paediatric Neurology: EJPN
https://www.readbyqxmd.com/read/28617836/molecular-diversity-and-frequency-of-the-diarrheagenic-enteric-protozoan-giardia-duodenalis-and-cryptosporidium-spp-in-a-hospital-setting-in-northern-spain
#9
José Manuel Azcona-Gutiérrez, Aida de Lucio, Marta Hernández-de-Mingo, Concepción García-García, Luis Miguel Soria-Blanco, Lucía Morales, María Aguilera, Isabel Fuentes, David Carmena
BACKGROUND: Human giardiosis and cryptosporidiosis are caused by the enteric protozoan parasites Giardia duodenalis and Cryptosporidium spp. Both pathogens are major contributors to the global burden of diarrhoeal disease, affecting primarily children and immunodebilitated individuals in resource-poor settings. Giardiosis and cryptosporidiosis also represent an important, often underestimate, public health threat in developed countries. In Spain only limited information is currently available on the epidemiology of these infections...
2017: PloS One
https://www.readbyqxmd.com/read/28613188/automated-spirometry-quality-assurance-supervised-learning-from-multiple-experts
#10
Filip Velickovski, Robert Marti, Luigi Ceccaroni, Felip Burgos, Concepcion Gistau, Xavier Alsina-Restoy, Josep Roca
Forced spirometry testing is gradually becoming available across different healthcare tiers including primary care. It has been demonstrated in earlier work that commercially available spirometers are not fully able to assure the quality of individual spirometry manoeuvres. Thus a need to expand the availability of high quality spirometry assessment beyond specialist pulmonary centres has arisen. In this work we propose a method to select and optimise a classifier using supervised learning techniques by learning from previously classified forced spirometry tests from a group of experts...
June 8, 2017: IEEE Journal of Biomedical and Health Informatics
https://www.readbyqxmd.com/read/28609331/evaluation-and-impact-of-the-advanced-pediatric-life-support-course-in-the-care-of-pediatric-emergencies-in-spain
#11
Javier Benito, Carlos Luaces-Cubells, Santiago Mintegi, Ignacio Manrique Martínez, Mercedes De la Torre Espí, Concepción Miguez Navarro, Paula Vazquez López, Carmen Campos Calleja, Francesc Ferres Serrat, María Teresa Alonso Salas, Javier González Del Rey
INTRODUCTION: The Advanced Pediatric Life Support (APLS) course was introduced in the training of professionals who care for pediatric emergencies in Spain in 2005. OBJECTIVE: To analyze the impact of the APLS course in the current clinical practice in Spanish PEDs. METHODS: The directors of APLS courses were asked about information regarding the courses given to date, especially on the results of the satisfaction survey completed by students at the end of the course...
June 12, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28607419/two-step-atp-driven-opening-of-cohesin-head
#12
Íñigo Marcos-Alcalde, Jesús I Mendieta-Moreno, Beatriz Puisac, María Concepción Gil-Rodríguez, María Hernández-Marcos, Diego Soler-Polo, Feliciano J Ramos, José Ortega, Juan Pié, Jesús Mendieta, Paulino Gómez-Puertas
The cohesin ring is a protein complex composed of four core subunits: Smc1A, Smc3, Rad21 and Stag1/2. It is involved in chromosome segregation, DNA repair, chromatin organization and transcription regulation. Opening of the ring occurs at the "head" structure, formed of the ATPase domains of Smc1A and Smc3 and Rad21. We investigate the mechanisms of the cohesin ring opening using techniques of free molecular dynamics (MD), steered MD and quantum mechanics/molecular mechanics MD (QM/MM MD). The study allows the thorough analysis of the opening events at the atomic scale: i) ATP hydrolysis at the Smc1A site, evaluating the role of the carboxy-terminal domain of Rad21 in the process; ii) the activation of the Smc3 site potentially mediated by the movement of specific amino acids; and iii) opening of the head domains after the two ATP hydrolysis events...
June 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28606934/cell-lineage-of-timed-cohorts-of-tbx6-expressing-cells-in-wild-type-and-tbx6-mutant-embryos
#13
Daniel Concepcion, Andrew J Washkowitz, Akiko DeSantis, Phillip Ogea, Jason I Yang, Nataki C Douglas, Virginia E Papaioannou
Tbx6 is a T-box transcription factor with multiple roles in embryonic development as evidenced by dramatic effects on mesoderm cell fate determination, left/right axis determination, and somite segmentation in mutant mice. The expression of Tbx6 is restricted to the primitive streak and presomitic mesoderm, but some of the phenotypic features of mutants are not easily explained by this expression pattern. We have used genetically-inducible fate mapping to trace the fate of Tbx6-expressing cells in wild type and mutant embryos to explain some of the puzzling features of the mutant phenotype...
June 12, 2017: Biology Open
https://www.readbyqxmd.com/read/28606092/hyperlipidemic-microenvironment-conditionates-damage-mechanisms-in-human-chondrocytes-by-oxidative-stress
#14
Daniel Medina-Luna, Mónica Guadalupe Santamaría-Olmedo, Yessica Zamudio-Cuevas, Karina Martínez-Flores, Javier Fernández-Torres, Gabriela Angélica Martínez-Nava, Denise Clavijo-Cornejo, Cristina Hernández-Díaz, Anell Olivos-Meza, Luis Enrique Gomez-Quiroz, María Concepción Gutiérrez-Ruiz, Carlos Pineda, Francisco Blanco, Anthony M Reginato, Alberto López-Reyes
BACKGROUND: Currently, two pathogenic pathways describe the role of obesity in osteoarthritis (OA); one through biomechanical stress, and the other by the contribution of systemic inflammation. The aim of this study was to evaluate the effect of free fatty acids (FFA) in human chondrocytes (HC) expression of proinflammatory factors and reactive oxygen species (ROS). METHODS: HC were exposed to two different concentrations of FFA in order to evaluate the secretion of adipokines through cytokines immunoassays panel, quantify the protein secretion of FFA-treated chondrocytes, and fluorescent cytometry assays were performed to evaluate the reactive oxygen species (ROS) production...
June 12, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28604361/synthetic-scaffolds-with-full-pore-interconnectivity-for-bone-regeneration-prepared-by-supercritical-foaming-using-advanced-biofunctional-plasticizers
#15
Aurelio Salerno, Sara Diéguez, Luis Diaz-Gomez, Jose Luis Gomez-Amoza, Beatriz Magariños, Angel Concheiro, Concepcion Domingo, Carmen Alvarez-Lorenzo, Carlos A García-González
Supercritical foaming allows for the solvent-free processing of synthetic scaffolds for bone regeneration. However, the control on the pore interconnectivity and throat pore size with this technique still needs to be improved. The use of plasticizers may help to overcome these limitations. Eugenol, a GRAS natural compound extracted from plants, is proposed in this work as an advanced plasticizer with bioactive properties. Eugenol-containing poly(ɛ-caprolactone) (PCL) scaffolds were obtained by supercritical foaming (20...
June 12, 2017: Biofabrication
https://www.readbyqxmd.com/read/28598971/expression-of-inhibitory-receptors-and-polyfunctional-responses-of-t-cells-are-linked-to-the-risk-of-congenital-transmission-of-t-cruzi
#16
Adriana Egui, Paola Lasso, María Carmen Thomas, Bartolomé Carrilero, John Mario González, Adriana Cuéllar, Manuel Segovia, Concepción Judith Puerta, Manuel Carlos López
Congenital T. cruzi infections involve multiple factors in which complex interactions between the parasite and the immune system of pregnant women play important roles. In this study, we used an experimental murine model of chronic infection with T. cruzi to evaluate the changes in the expression of inhibitory receptors and the polyfunctionality of T cells during gestation and their association with congenital transmission rate of T. cruzi infection. The results showed that pregnant naïve mice had a higher percentage of CD4+ and CD8+ T cells that expressed inhibitory receptors than cells from non-pregnant naïve mice...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28591312/identification-of-a-type-i-nitroreductase-gene-in-non-virulent-trypanosoma-rangeli
#17
Marjorie Montenegro, Claudia Cuervo, Constanza Cardenas, Silvia Duarte, Jenny R Díaz, M Carmen Thomas, Manuel C Lopez, Concepcion J Puerta
Trypanosomatid type I nitroreductases (NTRs), i.e., mitochondrial enzymes that metabolise nitroaromatic pro-drugs, are essential for parasite growth, infection, and survival. Here, a type I NTR of non-virulent protozoan Trypanosoma rangeli is described and compared to those of other trypanosomatids. The NTR gene was isolated from KP1(+) and KP1(-) strains, and its corresponding transcript and 5' untranslated region (5'UTR) were determined. Bioinformatics analyses and nitro-drug activation assays were also performed...
July 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591297/organization-of-professional-practices-against-intrafamily-violence-against-children-and-adolescents-in-the-institutional-context
#18
Gabriele Schek, Mara Regina Santos da Silva, Carl Lacharité, Maria Emília Nunes Bueno
Objective: to analyze based on the practitioners' discourse, the way they organize their practices confronting situations of intra-family violence against children and adolescents. Method: qualitative research carried out with 15 professionals who work in social and health services located in the southernmost of Brazil. Data were collected through semi-structured interviews, performed at the participants' workplace. We used a theoretical matrix to analyze the data, based on Institutional Ethnography and the technique of discursive textual analysis...
June 5, 2017: Revista Latino-americana de Enfermagem
https://www.readbyqxmd.com/read/28590517/flower-specialization-the-occluded-corolla-of-snapdragons-antirrhinum-exhibits-two-pollinator-niches-of-large-long-tongued-bees
#19
Pablo Vargas, Isabel Liberal, Concepción Ornosa, Jose María Gómez
• Flower specialization of angiosperms includes the occluded corollas of snapdragons (Antirrhinum and some relatives), which have been postulated to be one of the most efficient structures to physical limit access to pollinators. The Iberian Peninsula harbours the highest number of species (18 Iberian of the 20 species of Antirrhinum) that potentially share similar pollinator fauna. • Crossing experiments with 18 Iberian species from this study and literature revealed a general pattern of self-incompatibility-failure in this SI system has been also observed in a few plants-, which indicates the need of pollinator agents in Antirrhinum pollination...
June 7, 2017: Plant Biology
https://www.readbyqxmd.com/read/28589124/a-multidisciplinary-phenotyping-and-genotyping-analysis-of-a-mapping-population-enables-quality-to-be-combined-with-yield-in-rice
#20
Mariafe Calingacion, Roland Mumm, Kevin Tan, Lenie Quiatchon-Baeza, Jeanaflor C T Concepcion, Jos A Hageman, Sangeeta Prakash, Melissa Fitzgerald, Robert D Hall
In this study a mapping population (F8) of ca 200 progeny from a cross between the commercial rice varieties Apo and IR64 has been both genotyped and phenotyped. A genotyping-by-sequencing approach was first used to identify 2,681 polymorphic SNP markers which gave dense coverage of the genome with a good distribution across all 12 chromosomes. The coefficient of parentage was also low, at 0.13, confirming that the parents are genetically distant from each other. The progeny, together with both parents, were grown under irrigated and water restricted conditions in a randomised block design...
2017: Frontiers in Molecular Biosciences
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