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https://www.readbyqxmd.com/read/29776931/shuffling-the-neutral-drift-of-unspecific-peroxygenase-in-saccharomyces-cerevisiae
#1
Javier Martin-Diaz, Carmen Paret, Eva García-Ruiz, Patricia Molina-Espeja, Miguel Alcalde
Unspecific peroxygenase (UPO) is a highly promiscuous biocatalyst and its selective mono(per)oxygenase activity makes it useful for many synthetic chemistry applications. Among the broad repertory of library creation methods for directed enzyme evolution, genetic drift allows neutral mutations to be accumulated gradually within a polymorphic network of variants. In this study, we conducted a campaign of genetic drift with UPO in Saccharomyces cerevisiae so that neutral mutations were simply added and recombined in vivo With low mutational loading and an activity threshold of 45% of the parent's native function, mutant libraries enriched in folded and active UPO variants were generated...
May 18, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29775805/new-subsolid-pulmonary-nodules-in-lung-cancer-screening-a-brief-report-of-the-nelson-trial
#2
Joan E Walter, Marjolein A Heuvelmans, Uraujh Yousaf-Khan, Monique D Dorrius, Erik Thunnissen, Anna Schermann, Harry J M Groen, Carlijn M van der Aalst, Kristiaan Nackaerts, Rozemarijn Vliegenthart, Harry J de Koning, Matthijs Oudkerk
INTRODUCTION: Low-dose computed tomography (LDCT) lung cancer screening is recommended in the United States. While new solid nodules after baseline screening have a high lung cancer probability at small size and require lower size cutoff values than baseline nodules, there only is limited evidence on management of new subsolid nodules. METHODS: Within the Dutch-Belgian randomized controlled LDCT lung cancer screening trial (NELSON), 7557 participants underwent baseline screening between April 2004 and December 2006...
May 15, 2018: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/29769056/report-on-an-international-workshop-on-kangaroo-mother-care-lessons-learned-and-a-vision-for-the-future
#3
Adriano Cattaneo, Adidja Amani, Nathalie Charpak, Socorro De Leon-Mendoza, Sarah Moxon, Somashekhar Nimbalkar, Giorgio Tamburlini, Julieta Villegas, Anne-Marie Bergh
BACKGROUND: Globally, complications of prematurity are the leading cause of death in children under five. Preterm infants who survive their first month of life are at greater risk for various diseases and impairments in infancy, childhood and later life, representing a heavy social and economic burden for families, communities and health and social systems. Kangaroo mother care (KMC) is recommended as a beneficial and effective intervention for improving short- and long-term preterm birth outcomes in low- and high-income settings...
May 16, 2018: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29764915/remote-physiological-monitoring-in-an-austere-environment-a-future-for-battlefield-care-provision
#4
Matthew J Smyth, J A Round, A J Mellor
Wearable technologies are making considerable advances into the mainstream as they become smaller and more user friendly. The global market for such devices is forecasted to be worth over US$5 billion in 2018, with one in six people owning a device. Many professional sporting teams use self-monitoring to assess physiological parameters and work rate on the pitch, highlighting the potential utility for military command chains. As size of device reduces and sensitivity improves, coupled with remote connectivity technology, integration into the military environment could be relatively seamless...
May 14, 2018: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/29762070/developing-a-palliative-care-competency-framework-for-health-professionals-and-volunteers-the-nova-scotian-experience
#5
Meg McCallum, Janet Carver, David Dupere, Sharon Ganong, J David Henderson, Ann McKim, Lisa McNeil-Campbell, Holly Richardson, Judy Simpson, Cheryl Tschupruk, Heather Jewers
BACKGROUND AND OBJECTIVES: In 2014, Nova Scotia released a provincial palliative care strategy and implementation working groups were established. The Capacity Building and Practice Change Working Group, comprised of health professionals, public advisors, academics, educators, and a volunteer supervisor, was asked to select palliative care education programs for health professionals and volunteers. The first step in achieving this mandate was to establish competencies for health professionals and volunteers caring for patients with life-limiting illness and their families and those specializing in palliative care...
May 15, 2018: Journal of Palliative Medicine
https://www.readbyqxmd.com/read/29761692/visualizing-the-zero-potential-line-of-bipolar-electrodes-with-arbitrary-geometry
#6
Meng Li, Shasha Liu, Yingyan Jiang, Wei Wang
In a typical bipolar electrochemistry (BPE) configuration, voltage applied between the two driving electrodes induced a potential drop through solution filled in the microchannel, resulting in interfacial potential difference between solution and BPE varied along the BPE. In the present work, we employed a recently-developed plasmonic imaging technique to map the distribution of surface potential of bipolar electrodes with various geometries including round, triangle, hexagon, star and rhombus shapes under non-faradaic charging process, from which the line of zero potential (LZP) was visualized and determined...
May 15, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29753344/challenges-in-research-and-health-technology-assessment-of-rare-disease-technologies-report-of-the-ispor-rare-disease-special-interest-group
#7
Sandra Nestler-Parr, Daria Korchagina, Mondher Toumi, Chris L Pashos, Christopher Blanchette, Elizabeth Molsen, Thomas Morel, Steven Simoens, Zoltán Kaló, Ruediger Gatermann, William Redekop
BACKGROUND: Successful development of new treatments for rare diseases (RDs) and their sustainable patient access require overcoming a series of challenges related to research and health technology assessment (HTA). These impediments, which may be unique to RDs or also apply to common diseases but are particularly pertinent in RDs, are diverse and interrelated. OBJECTIVE: To develop for the first time a catalog of primary impediments to RD research and HTA, and to describe the cause and effect of individual challenges...
May 2018: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/29753070/iterative-algorithm-guided-design-of-massive-strain-libraries-applied-to-itaconic-acid-production-in-yeast
#8
Eric M Young, Zheng Zhao, Bianca E M Gielesen, Liang Wu, D Benjamin Gordon, Johannes A Roubos, Christopher A Voigt
Metabolic engineering requires multiple rounds of strain construction to evaluate alternative pathways and enzyme concentrations. Optimizing multigene pathways stepwise or by randomly selecting enzymes and expression levels is inefficient. Here, we apply methods from design of experiments (DOE) to guide the construction of strain libraries from which the maximum information can be extracted without sampling every possible combination. We use Saccharomyces cerevisiae as a host for a novel six-gene pathway to itaconic acid, selected by comparing alternative shunt pathways that bypass the mitochondrial TCA cycle...
May 9, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/29746849/characterisation-of-re-entrant-circuit-or-rotational-activity-in-vitro-using-the-hl1-6-myocyte-cell-line
#9
Charles Houston, Konstantinos N Tzortzis, Caroline Roney, Andrea Saglietto, David S Pitcher, Chris Cantwell, Rasheda A Chowdhury, Fu Siong Ng, Nicholas S Peters, Emmanuel Dupont
Fibrillation is the most common arrhythmia observed in clinical practice. Understanding of the mechanisms underlying its initiation and maintenance remains incomplete. Functional re-entries are potential drivers of the arrhythmia. Two main concepts are still debated, the "leading circle" and the "spiral wave or rotor" theories. The homogeneous subclone of the HL1 atrial-derived cardiomyocyte cell line, HL1-6, spontaneously exhibits re-entry on a microscopic scale due to its slow conduction velocity and the presence of triggers, making it possible to examine re-entry at the cellular level...
May 7, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/29746344/nutritional-considerations-in-pediatric-pancreatitis-a-position-paper-from-the-naspghan-pancreas-committee-espghan-pancreas-working-group
#10
Maisam Abu-El-Haija, Aliye Uc, Steven L Werlin, A Jay Freeman, Miglena Georgieva, Danijela Jojkić - Pavkov, Daina Kalnins, Brigitte Kochavi, Bart G P Koot, Stephanie Van Biervliet, Jaroslaw Walkowiak, Michael Wilschanski, Veronique D Morinville
OBJECTIVES: Wide variations exist in how physicians manage the nutritional aspects of children affected by acute pancreatitis (AP), acute recurrent pancreatitis (ARP) and chronic (CP) pancreatitis. Better consensus for optimal management is needed. METHODS: This consensus statement on nutrition in pediatric pancreatic diseases was developed through a joint ESPGHAN-NASPGHAN working group that performed an evidence-based search of the literature on nutrition in AP, ARP, and CP with a focus on pediatrics...
May 8, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29745031/trypanosoma-cruzi-biochemical-changes-and-cell-death-induced-by-an-organometallic-platinum-based-compound
#11
M Florencia Mosquillo, Lucía Bilbao, Fabricio Hernández, Florencia Tissot, Dinorah Gambino, Beatriz Garat, Leticia Pérez-Díaz
Chagas disease is an endemic illness in Latin America caused by the parasite Trypanosoma cruzi. Current chemotherapies are old and inadequate, and the emergence of drug resistant strains underscores the need of new drugs. Platinum-based complexes have been shown to be a promising approach against parasitic diseases. In this work, the effect of 1,1'-bis(diphenylphosphino)ferrocene pyridine-2-thiolate-1-oxide Pt(II) hexafluorophosphate, Pt-dppf-mpo, was studied on T. cruzi. A promising anti-trypanosomal activity was determined for the CL Brener strain with a low cytotoxicity determined using in vitro cultured mammal cells...
May 9, 2018: Chemical Biology & Drug Design
https://www.readbyqxmd.com/read/29743783/dental-technicians-pneumoconiosis-illness-behind-a-healthy-smile-case-series-of-a-reference-center-in-turkey
#12
Nur Şafak Alici, Ayşe Coşkun Beyan, Yücel Demıral, Arif Çimrin
Background: Dental laboratories include many hazards and risks. Dental technicians working in an unfavorable work environment in Turkey and other parts of the world may develop pneumoconiosis as a result of exposure to dust, depending on exposure time. In this study, we aimed to investigate the clinical and laboratory findings of dental technicians. Materials and Methods: The study consists of a case series. Between 2013 and 2016, a total of 70 who were working as a dental technician and referred to our clinic with suspicion of occupational disease were evaluated...
January 2018: Indian Journal of Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/29742756/applying-user-centered-design-methods-to-the-development-of-an-mhealth-application-for-use-in-the-hospital-setting-by-patients-and-care-partners
#13
Brittany Couture, Elizabeth Lilley, Frank Chang, Ann DeBord Smith, Jessica Cleveland, Awatef Ergai, Zachary Katsulis, James Benneyan, Esteban Gershanik, David W Bates, Sarah A Collins
INTRODUCTION:  Developing an optimized and user-friendly mHealth application for patients and family members in the hospital environment presents unique challenges given the diverse patient population and patients' various states of well-being. OBJECTIVE:  This article describes user-centered design methods and results for developing the patient and family facing user interface and functionality of MySafeCare, a safety reporting tool for hospitalized patients and their family members...
April 2018: Applied Clinical Informatics
https://www.readbyqxmd.com/read/29735903/pharmaceutical-machine-learning-virtual-high-throughput-screens-identifying-promising-and-economical-small-molecule-inhibitors-of-complement-factor-c1s
#14
Jonathan J Chen, Lyndsey N Schmucker, Donald P Visco
When excessively activated, C1 is insufficiently regulated, which results in tissue damage. Such tissue damage causes the complement system to become further activated to remove the resulting tissue damage, and a vicious cycle of activation/tissue damage occurs. Current Food and Drug Administration approved treatments include supplemental recombinant C1 inhibitor, but these are extremely costly and a more economical solution is desired. In our work, we have utilized an existing data set of 136 compounds that have been previously tested for activity against C1...
May 7, 2018: Biomolecules
https://www.readbyqxmd.com/read/29732736/australian-recommendations-for-the-integration-of-emergency-care-for-older-people-consensus-statement
#15
Judy A Lowthian, Glenn Arendts, Edward Strivens
OBJECTIVES: Management of older patients during acute illness or injury does not occur in isolation in emergency departments. We aimed to develop a collaborative Consensus Statement to enunciate principles of integrated emergency care. METHODS: Briefing notes, informed by research and evidence reviews, were developed and evaluated by a Consensus Working Party comprising cross-specialty representation from clinical experts, service providers, consumers and policymakers...
May 7, 2018: Australasian Journal on Ageing
https://www.readbyqxmd.com/read/29729969/the-effect-of-the-orientation-and-the-height-of-periodic-sub-micrometric-texturing-on-dropwise-condensation
#16
Nicolas Pionnier, Julie Vera, Elise Contraires, Stéphane Benayoun, Rémi Berger, Stéphane Valette
Controlling condensation conditions by surface topography is of prime interest. The aim of this work is to investigate the behavior of water droplets condensing on oriented sub-micrometric structures representing ripples with wavelengths around 800 nm. Droplet behavior was studied on different ripples heights and on untextured surfaces. It was specifically looked at how the presence of ripples creates geometrical confinement, and how that influences the deformation and the orientation of single droplets. Results show that the condensed droplets follow the orientation of textured features, especially with high structures height (150 nm)...
April 30, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29728777/colorimetric-and-fluorometric-determination-of-uric-acid-based-on-the-use-of-nitrogen-doped-carbon-quantum-dots-and-silver-triangular-nanoprisms
#17
Yanying Wang, Yan Yang, Wei Liu, Fang Ding, Qingbiao Zhao, Ping Zou, Xianxiang Wang, Hanbing Rao
A dual-read detection system is described for non-enzymatic and non-aggregation based analysis of uric acid (UA). Silver triangular nanoprisms (AgTNPs) were used as colorimetric probes, while the reduction in the fluorescence of nitrogen-doped carbon quantum dots (N-CQDs) served as the fluorometric readout. The absorption band of the AgTNPs overlaps the emission band of N-CQDs (with a peak at 440 nm). Therefore, fluorescence is reduced owing to an inner filter effect. The AgTNPs are etched if exposed to H2 O2 , and round nanodiscs are formed...
May 4, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29726415/going-mobile-an-empirical-model-for-explaining-successful-information-logistics-in-ward-rounds
#18
Moritz Esdar, Jan-David Liebe, Birgit Babitsch, Ursula Hübner
BACKGROUND: Medical ward rounds are critical focal points of inpatient care that call for uniquely flexible solutions to provide clinical information at the bedside. While this fact is undoubted, adoption rates of mobile IT solutions remain rather low. OBJECTIVES: Our goal was to investigate if and how mobile IT solutions influence successful information provision at the bedside, i.e. clinical information logistics, as well as to shed light at socio-organizational factors that facilitate adoption rates from a user-centered perspective...
2018: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/29721776/formation-of-lamellar-bodies-in-rat-liver-mitochondria-in-hyperthyroidism
#19
Natalya I Venediktova, Lubov L Pavlik, Natalia V Belosludtseva, Natalya V Khmil, Svetlana V Murzaeva, Galina D Mironova
In the present work, ultrastructural changes of rat liver mitochondria in hyperthyroidism were studied. Hyperthyroidism was induced in male Wistar rats by daily administration of 100 μg thyroxin per 100 g body weight for 5 days. The level of triiodothyronine and thyroxine increased 3- and 4-fold, respectively, in comparison with the same parameters in the control group, indicating the development of hyperthyroidism in experimental animals. It was found that under this experimental pathology 58% of the mitochondria are swollen, with their matrix enlightened, as compared to the control...
May 3, 2018: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/29718792/a-younger-man-with-localized-prostate-cancer-asks-which-type-of-radiation-is-right-for-me
#20
Justin E Bekelman
The Oncology Grand Rounds series is designed to place original reports published in the Journal into clinical context. A case presentation is followed by a description of diagnostic and management challenges, a review of the relevant literature, and a summary of the authors' suggested management approaches. The goal of this series is to help readers better understand how to apply the results of key studies, including those published in Journal of Clinical Oncology, to patients seen in their own clinical practice...
May 2, 2018: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
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