keyword
MENU ▼
Read by QxMD icon Read
search

acvs

keyword
https://www.readbyqxmd.com/read/29034838/formulation-optimization-and-evaluation-of-organogel-for-topical-delivery-of-acyclovir
#1
Smita Bonde, Chandrakant Bonde, Nisarg Mavani
BACKGROUND: The conventional acyclovir topical therapy has a low efficacy, due to the lack of penetration of a sufficient amount of drug to the target site. OBJECTIVE: The aim of this work was to formulate and optimize organogel containing acyclovir to enhance the penetration and retention time of acyclovir in the basal epidermis, site of Herpes simplex virus infections. METHODS: Microemulsion based organogel containing acyclovir was developed using the combination of surfactants, polar and nonpolar solvents...
October 13, 2017: Current Drug Delivery
https://www.readbyqxmd.com/read/28985573/antibody-based-immunotherapy-of-aciclovir-resistant-ocular-herpes-simplex-virus-infections
#2
Dirk Bauer, Jessica Keller, Mira Alt, Axel Schubert, Ulrich Wilhelm Aufderhorst, Vivien Palapys, Maren Kasper, Christiane Silke Heilingloh, Ulf Dittmer, Björn Laffer, Anna Maria Eis-Hübinger, Georges M Verjans, Arnd Heiligenhaus, Michael Roggendorf, Adalbert Krawczyk
The increasing incidence of aciclovir- (ACV) resistant strains in patients with ocular herpes simplex virus (HSV) infections is a major health problem in industrialized countries. In the present study, the humanized monoclonal antibody (mAb) hu2c targeting the HSV-1/2 glycoprotein B was examined for its efficacy towards ACV-resistant infections of the eye in the mouse model of acute retinal necrosis (ARN). BALB/c mice were infected by microinjection of an ACV-resistant clinical isolate into the anterior eye chamber to induce ARN and systemically treated with mAb hu2c at 24h prior (pre-exposure prophylaxis) or at 24, 40, and 56h after infection (post-exposure immunotherapy)...
October 3, 2017: Virology
https://www.readbyqxmd.com/read/28982161/novel-multipurpose-pod-intravaginal-ring-for-the-prevention-of-hiv-hsv-and-unintended-pregnancy-pharmacokinetic-evaluation-in-a-macaque-model
#3
James M Smith, John A Moss, Priya Srinivasan, Irina Butkyavichene, Manjula Gunawardana, Rob Fanter, Christine S Miller, Debbie Sanchez, Flora Yang, Shanon Ellis, Jining Zhang, Mark A Marzinke, Craig W Hendrix, Amita Kapoor, Marc M Baum
Globally, women bear an uneven burden for sexual HIV acquisition. Results from two clinical trials evaluating intravaginal rings (IVRs) delivering the antiretroviral agent dapivirine have shown that protection from HIV infection can be achieved with this modality, but high adherence is essential. Multipurpose prevention technologies (MPTs) can potentially increase product adherence by offering protection against multiple vaginally transmitted infections and unintended pregnancy. Here we describe a coitally independent, long-acting pod-IVR MPT that could potentially prevent HIV and HSV infection as well as unintended pregnancy...
2017: PloS One
https://www.readbyqxmd.com/read/28965164/does-the-disruption-of-horizontal-anterior-ciliary-vessels-affect-the-blood-aqueous-barrier-function
#4
Meihua Pan, Mei Yang, Renyi Xie, Zhimin Zhao, Xingxing Huang
PURPOSE: To investigate the significance of the anterior ciliary vessels (ACVs) preservation during the conventional horizontal strabismus surgery. METHODS: Patients (≥ 8 years) with horizontal strabismus were randomly allocated into group 1 (with ACV preservation) and group 2 (without ACV preservation). The surgical eyes in group 1 were further divided into group A (one rectus muscle operated) and group B (two rectus muscles operated). Similarly, eyes in group 2 were divided into group C (one rectus muscle operated) and group D (two rectus muscles operated)...
September 30, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/28939476/recurrent-herpetic-keratitis-despite-antiviral-prophylaxis-a-virological-and-pharmacological-study
#5
Antoine Rousseau, David Boutolleau, Karine Titier, Tristan Bourcier, Christophe Chiquet, Michel Weber, Joseph Colin, Julie Gueudry, Mohamed M'Garrech, Bahram Bodaghi, Sonia Burrel, Henri Agut, Claire Deback, Marc Labetoulle
Recurrent herpes simplex keratitis (HSK) is a leading infectious cause of blindness in industrialized countries. Antiviral prophylaxis (AVP) may fail to prevent recurrence of HSK due to viral resistance, inadequate dosing, or poor patient compliance. In this prospective multicenter study, we enrolled immunocompetent patients with recurrent HSK despite AVP. Ocular samples were tested by PCR for herpes simplex virus 1 (HSV-1). HSV-1 drug resistance was assessed with a genotypic assay based on UL23 and UL30 gene sequencing...
September 20, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28933042/anterior-chamber-changes-after-implantable-collamer-lens-implantation-in-high-myopia-using-pentacam-a-prospective-study
#6
Mohamed Nagy Elmohamady, Walid Abdelghaffar
INTRODUCTION: The objective of this study was to evaluate the changes of the anterior chamber (AC) of the eye after implantable Collamer lens (ICL) implantation in high myopia by using the Pentacam. METHODS: The prospective study included 34 high myopic patients (34 eyes). All patients were treated with Visian implantable Collamer lens (ICL) (Staar Surgical Co.) implantation. The Pentacam (Oculus) was used for all cases preoperatively and 1, 3 and 6 months postoperatively...
September 20, 2017: Ophthalmology and Therapy
https://www.readbyqxmd.com/read/28917799/a-reagentless-and-reusable-electrochemical-aptamer-based-sensor-for-rapid-detection-of-ampicillin-in-complex-samples
#7
Zhi-Gang Yu, Rebecca Y Lai
We report the design and fabrication of a "signal-on" electrochemical aptamer-based (E-AB) sensor for detection of ampicillin. The signaling of the sensor is based on target binding-induced changes in the conformation and flexibility of the methylene blue-modified aptamer probe. The sensor's response is fast; signal saturation can be reached in ~ 200s. Since all the sensor components are surface-immobilized, it is regenerable and can be reused for at least three times. It has demonstrated good specificity and is capable of differentiating between ampicillin and structurally similar antibiotics such as amoxicillin...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28832743/effects-of-hemodialysis-on-corneal-and-anterior-chamber-morphometry-and-intraocular-pressure-in-patients-with-end-stage-renal-disease
#8
Mehtap Caglayan, Pinar Kosekahya, Tamer Takmaz, Alpaslan Altunoglu, Berna Ayan, Cemile Ucgul Atilgan, Betul Seher Uysal
PURPOSE: To evaluate the effects of hemodialysis (HD) on corneal and anterior chamber morphometry, as well as intraocular pressure (IOP) in patients with end-stage renal disease. METHODS: Fifty right eyes were examined 30 minutes before and after HD. IOP was measured with a Goldmann applanation tonometer, and Ehlers' formula was used to calculate the corrected IOP values. The central corneal thickness (CCT), corneal volume (CV), keratometric values, anterior chamber depth (ACD), aqueous depth (AQD), anterior chamber volume (ACV), and anterior chamber angle (ACA) in the nasal and temporal quadrants were measured with a Sirius anterior segment analysis system...
June 2017: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28829913/virtual-screening-of-acyclovir-derivatives-as-potential-antiviral-agents-design-synthesis-and-biological-evaluation-of-new-acyclic-nucleoside-protides
#9
Marco Derudas, Christophe Vanpouille, Davide Carta, Sonia Zicari, Graciela Andrei, Robert Snoeck, Andrea Brancale, Leonid Margolis, Jan Balzarini, Christopher McGuigan
Following our findings on the anti-human immunodeficiency virus (HIV) activity of acyclovir (ACV) phosphate prodrugs, we herein report the ProTide approach applied to a series of acyclic nucleosides aimed at the identification of novel and selective antiviral, in particular anti-HIV agents. Acyclic nucleoside analogues used in this study were identified through a virtual screening using HIV-reverse transcriptase (RT), adenylate/guanylate kinase, and human DNA polymerase γ. A total of 39 new phosphate prodrugs were synthesized and evaluated against HIV-1 (in vitro and ex vivo human tonsillar tissue system) and human herpes viruses...
September 28, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28761568/the-effects-of-ramadan-fasting-on-anterior-segment-parameters-visual-acuity-and-intraocular-pressures-of-the-eye
#10
Ozlem Barut Selver, Melis Palamar, Kevser Gerceker, Sait Egrilmez, Ayse Yagci
OBJECTIVE: It is aimed to determine whether fasting during Ramadan has any significant effect on anterior chamber parameters, visual acuity and intraocular pressures. METHODS: 31 fasting (Group 1) and 30 non-fasting healthy volunteers (Group 2) were enrolled. All cases underwent an ophthalmological examination and anterior segment parameter evaluation (central corneal thickness (CCT), anterior chamber depth (ACD), anterior chamber volume (ACV), anterior chamber angle (ACA), pupil size) with Pentacam before and after the breaking of the Ramadan fast in Group 1, before and after dinner in Group 2...
2017: Open Ophthalmology Journal
https://www.readbyqxmd.com/read/28717975/development-of-a-novel-polymeric-nanocomposite-complex-for-drugs-with-low-bioavailability
#11
Mduduzi N Sithole, Yahya E Choonara, Lisa C du Toit, Pradeep Kumar, Thashree Marimuthu, Pierre P D Kondiah, Viness Pillay
Semi-synthetic biopolymer complex (SSBC) nanoparticles were investigated as a potential oral drug delivery system to enhance the bioavailability of a poorly water-soluble model drug acyclovir (ACV). The SSBCs were prepared from cross-linking of hydroxyl groups on hyaluronic acid (HA) with poly(acrylic acid) (PAA) resulting in ether linkages. Thereafter, conjugation of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) onto HA-PAA was accomplished using a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)-promoted coupling reaction...
July 17, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28711432/inactivated-orf-virus-shows-disease-modifying-antiviral-activity-in-a-guinea-pig-model-of-genital-herpesvirus-infection
#12
Astrid Friebe, Angela Siegling, Olaf Weber
BACKGROUND: Inactivated Orf virus (iORFV) has been used as a preventative as well as a therapeutic immunomodulator in veterinary medicine in different species. iORFV elicits strong effects on cytokine secretion in mice and human immune cells leading to an auto-regulated loop of initial up-regulation of inflammatory and Th1-related cytokines followed by Th2-related cytokines that attenuate immunopathology. The therapeutic potential of iORFV has been recognized in several models for difficult-to-treat disease areas such as chronic viral diseases, liver fibrosis or various forms of cancer...
June 27, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28703303/terminally-truncated-isopenicillin-n-synthase-generates-a-dithioester-product-evidence-for-a-thioaldehyde-intermediate-during-catalysis-and-a-new-mode-of-reaction-for-non-heme-iron-oxidases
#13
Luke A McNeill, Toby J N Brown, Malkit Sami, Ian J Clifton, Nicolai I Burzlaff, Timothy D W Claridge, Robert M Adlington, Jack E Baldwin, Peter J Rutledge, Christopher J Schofield
Isopenicillin N synthase (IPNS) catalyses the four-electron oxidation of a tripeptide, l-δ-(α-aminoadipoyl)-l-cysteinyl-d-valine (ACV), to give isopenicillin N (IPN), the first-formed β-lactam in penicillin and cephalosporin biosynthesis. IPNS catalysis is dependent upon an iron(II) cofactor and oxygen as a co-substrate. In the absence of substrate, the carbonyl oxygen of the side-chain amide of the penultimate residue, Gln330, co-ordinates to the active-site metal iron. Substrate binding ablates the interaction between Gln330 and the metal, triggering rearrangement of seven C-terminal residues, which move to take up a conformation that extends the final α-helix and encloses ACV in the active site...
July 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28659899/a-novel-method-to-titrate-herpes-simplex-virus-1-hsv-1-using-laser-based-scanning-of-near-infrared-fluorophores-conjugated-antibodies
#14
Marco Fabiani, Dolores Limongi, Anna Teresa Palamara, Giovanna De Chiara, Maria Elena Marcocci
Among several strategies used for Herpes simplex virus (HSV) detection in biological specimens, standard plaque assay (SPA) remains the most reliable method to evaluate virus infectivity and quantify viral replication. However, it is a manual procedure, thereby affected by operator subjectivity, and it may be particularly laborious for multiple sample analysis. Here we describe an innovative method to perform the titration of HSV type 1 (HSV-1) in different samples, using the "In-Cell Western(TM)" Assay (ICW) from LI-COR, a quantitative immunofluorescence assay that exploits laser-based scanning of near infrared (NIR)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28655605/gadolinium-enhanced-7-0%C3%A2-t-magnetic-resonance-imaging-assessment-of-the-aqueous-inflow-in-rat-eyes-in%C3%A2-vivo
#15
Lu Li, Yuxiang Yuan, Liwen Chen, Mu Li, Pingting Ji, Jieling Gong, Yin Zhao, Hong Zhang
The goal of this study was to calculate the anterior chamber volume and assess aqueous inflow in rat eyes in vivo, under anesthetic condition. Gadolinium-contrast agent (Gd-DTPA, 234.5 mg/ml) was administered to Sprague-Dawley rat eyes via anterior chamber injection or instillation of 234.5 or 117.25 mg/ml Gd-DTPA in 0.2% azone as eye drops, and changes of Gd signal visualized by 7.0 T magnetic resonance imaging (MRI). The safety of local application of Gd-DTPA and azone were performed after MRI scanning...
September 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28647416/related-factors-of-asymmetrical-vein-sign-in-acute-middle-cerebral-artery-stroke-and-correlation-with-clinical-outcome
#16
Jinchao Yu, Liming Wang, Zhenzhi Li, Shanshan Wang, Guangbin Wang
OBJECTIVE: The aim of our study was to analyze the related factors of asymmetrical cortical vein sign (ACVS) and asymmetrical medullary vein sign (AMVS) on susceptibility-weighted imaging (SWI) in patients with acute middle cerebral artery (MCA) stroke and whether their presence can be used as an independent predictor for clinical outcome. METHODS: According to the presence of ACVS and AMVS on SWI, 124 patients with acute MCA stroke within 3 days were divided into several different groups...
October 2017: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
https://www.readbyqxmd.com/read/28629987/anti-herpes-simplex-virus-type-1-activity-of-houttuynoid-a-%C3%A2-a%C3%A2-flavonoid-from-houttuynia-cordata-thunb
#17
Ting Li, Libao Liu, Hongling Wu, Shaodan Chen, Qinchang Zhu, Hao Gao, Xiongtao Yu, Yi Wang, Wenhan Su, Xinsheng Yao, Tao Peng
Early events in herpes simplex virus type 1 (HSV-1) infection reactivate latent human immunodeficiency virus, Epstein-Barr virus, and human papillomavirus in the presence of acyclovir (ACV). The common use of nucleoside analog medications, such as ACV and pencyclovir, has resulted in the emergence of drug-resistant HSV-1 strains in clinical therapy. Therefore, new antiherpetics that can inhibit early events in HSV-1 infection should be developed. An example of this treatment is Houttuynia cordata Thunb. water extract, which can inhibit HSV-1 infection through multiple mechanisms...
June 17, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28602743/apple-cider-vinegar-boosted-immunomodulatory-and-health-promoting-effects-of-lactobacillus-casei-in-common-carp-cyprinus-carpio
#18
Roghieh Safari, Seyed Hossein Hoseinifar, Shabnam Nejadmoghadam, Mohsen Khalili
The present study was performed to investigate the immunomodulatory and health promoting effects of combined or singular administration of apple cider vinegar (ACV) and Lactobacillus casei in common carp (Cyprinus carpio) diet. An 8-week feeding trial was designed with following treatments: Control (basal diet), Pro (contains 10(7) CFU g(-1)L. casei), LACV (contains 1% ACV), HACV (contains 2% ACV), Pro + LACV (contains 10(7) CFU g(-1)L. casei plus 1% ACV) and Pro + HACV (contains 10(7) CFU g(-1)L...
August 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28600708/effect-of-refractive-status-on-valsalva-induced-anterior-segment-changes
#19
Necip Kara, Serhat Kenan
PURPOSE: To evaluate the effect of the Valsalva maneuver (VM) on anterior segment parameters and its association with refractive status. METHOD: In this prospective study, 120 eyes of 120 subjects were evaluated. Subjects were divided into three groups: myopic (n = 40 patients), hyperopic (n = 40 patients), and emmetropic (control group, n = 40 subjects). The anterior segment parameters, including keratometric, pachymetric, and anterior chamber parameters, were measured using the Sirius System (Costruzione Strumenti Oftalmici, Florence, Italy) before and during VM...
June 9, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28576448/stepwise-characterization-of-non-synonymous-mutations-in-the-hsv-1-thymidine-kinase-gene-by-different-functional-assays
#20
Marisa Kaspar, Kathrin Bohn-Wippert, Peter Bellstedt, Sabine Häfner, Matthias Görlach, Andreas Sauerbrei
Twenty amino acid substitutions in the thymidine kinase (TK) of clinical herpes simplex virus type 1 strains were assessed for conferring acyclovir (ACV) resistance. Site-directed mutagenesis, cell-free protein synthesis and protein expression in Escherichia coli were performed to obtain recombinant TK proteins, which were authenticated by Western blotting. A modified enzyme-linked immunosorbent assay (ELISA) was carried out to determine the phosphorylation activity of the mutants towards 5-bromo-2'-deoxyuridine (BrdU)...
May 30, 2017: Journal of Virological Methods
keyword
keyword
104344
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"