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Stromal derived factor

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https://www.readbyqxmd.com/read/28737959/influence-of-patient-related-factors-on-number-of-mesenchymal-stromal-cells-reached-after-in-vitro-culture-expansion-for-clinical-treatment
#1
Abbas Ali Qayyum, Kamal Preet Kaur, Anders Bruun Mathiasen, Mandana Haack-Sørensen, Annette Ekblond, Jens Kastrup
BACKGROUND: Number of stromal cells injected in patients with ischaemic heart disease (IHD) may be of importance for the treatment efficacy, which in turn may be influenced by various patient-related factors. In this study, we investigate whether patient-related factors influence the number of autologous stromal cells reached after in vitro culture expansion for clinical therapy. METHODS: Culture expansion data from 111 patients with IHD treated with autologous stromal cells in three clinical trials were used...
July 24, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28734802/co-transplantation-of-mesenchymal-and-neural-stem-cells-and-overexpressing-stromal-derived-factor-1-for-treating-spinal-cord-injury
#2
Andrew N Stewart, Griffin Kendziorski, Zachary M Deak, Dara J Brown, Matthew N Fini, Katherine L Copely, Julien Rossignol, Gary L Dunbar
Genetic engineering of mesenchymal stem cells (MSCs) and neuronal stem cells (NSCs) has been used to treat spinal cord injuries (SCI). As a mechanism of therapy, MSCs secrete high amounts of anti-inflammatory cytokines and trophic factors, while NSCs can differentiate into neuronal lineages and aid in tissue replacement. Additionally, the forced overexpression of secreted proteins can enhance the secretome of transplanted cells, which can increase therapeutic efficacy. This study utilized a combinational treatment consisting of MSCs, NSCs, and the forced overexpression of the chemokine stromal-derived factor-1 (SDF-1) from MSCs (SDF-1-MSCs), to treat a rat model of SCI...
July 19, 2017: Brain Research
https://www.readbyqxmd.com/read/28734730/multifaceted-c-x-c-chemokine-receptor-4-inhibition-interferes-with-anti-vascular-endothelial-growth-factor-therapy-induced-glioma-dissemination
#3
Jean-Pierre Gagner, Yasmeen Sarfraz, Valerio Ortenzi, Fawaz M Alotaibi, Luis A Chiriboga, Awab T Tayyib, Garry J Douglas, Eric Chevalier, Barbara Romagnoli, Gérald Tuffin, Michel Schmitt, Guillaume Lemercier, Klaus Dembowsky, David Zagzag
Resistance to antiangiogenic therapy glioblastoma (GBM) patients may involve hypoxia-induced expression of stromal cell-derived factor (SDF)-1α receptor C-X-C chemokine receptor 4 (CXCR4) on invading tumor, macrophage/microglial cells (MGCs), and glioma stem cells (GSCs). We determined whether antagonizing CXCR4 with peptide epitope mimetic POL5551 disrupts anti-vascular endothelial growth factor therapy-induced glioma growth and dissemination. Mice bearing orthotopic CT-2A or GL261 gliomas received POL5551 and/or anti-vascular endothelial growth factor antibody B20-4...
July 20, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28734678/improvement-of-adipose-tissue-derived-cells-by-low-energy-extracorporeal-shock-wave-therapy
#4
Eleni Priglinger, Christina M A P Schuh, Carolin Steffenhagen, Christoph Wurzer, Julia Maier, Sylvia Nuernberger, Wolfgang Holnthoner, Christiane Fuchs, Susanne Suessner, Dominik Rünzler, Heinz Redl, Susanne Wolbank
BACKGROUND: Cell-based therapies with autologous adipose tissue-derived cells have shown great potential in several clinical studies in the last decades. The majority of these studies have been using the stromal vascular fraction (SVF), a heterogeneous mixture of fibroblasts, lymphocytes, monocytes/macrophages, endothelial cells, endothelial progenitor cells, pericytes and adipose-derived stromal/stem cells (ASC) among others. Although possible clinical applications of autologous adipose tissue-derived cells are manifold, they are limited by insufficient uniformity in cell identity and regenerative potency...
July 19, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28733485/il-17-promotes-differentiation-of-splenic-lsk-lymphoid-progenitors-into-b-cells-following-plasmodium-yoelii-infection
#5
Debopam Ghosh, Susie L Brown, Jason S Stumhofer
Lineage(-)Sca-1(+)c-Kit(-) (LSK(-)) cells are a lymphoid progenitor population that expands in the spleen and preferentially differentiates into mature B cells in response to Plasmodium yoelii infection in mice. Furthermore, LSK(-) derived B cells can subsequently contribute to the ongoing immune response through the generation of parasite-specific Ab-secreting cells, as well as germinal center and memory B cells. However, the factors that promote their differentiation into B cells in the spleen postinfection are not defined...
July 21, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28724746/therapeutic-targeting-tumor-progression-locus-2-tpl2-activating-transcription-factor-4-atf4-chemokine-stromal-cell-derived-factor-%C3%AE-sdf1%C3%AE-axis-suppresses-diabetic-retinopathy
#6
De-Wei Lai, Keng-Hung Lin, Wayne Huey-Herng Sheu, Maw-Rong Lee, Chung-Yu Chen, Wen-Jane Lee, Yi-Wen Hung, Chin-Chang Shen, Tsung-Ju Chung, Shing-Hwa Liu, Meei-Ling Sheu
Rationale: Diabetic retinopathy is characterized by vasopermeability, vascular leakage, inflammation, blood-retinal barrier breakdown, capillary degeneration and neovascularization. However, the mechanisms underlying the association between diabetes and progression retinopathy remain unclear. Objective: Tumor progression locus 2 (TPL2), a serine-threonine protein kinase, exerts a pathological effect on vascular angiogenesis. This study investigated the role of N(ε)-(carboxymethyl)lysine (CML), a major advanced glycation end products, and the involved TPL2-related molecular signals in diabetic retinopathy using models of in vitro and in vivo and human samples...
July 19, 2017: Circulation Research
https://www.readbyqxmd.com/read/28721072/cxcl12-suppresses-cisplatin-induced-apoptosis-through-activation-of-jak2-stat3-signaling-in-human-non-small-cell-lung-cancer-cells
#7
Meng Wang, Tie Lin, Yicun Wang, Song Gao, Zhaoyang Yang, Xuan Hong, Gongyan Chen
AIMS: Poor efficacy of chemotherapy drugs in non-small-cell lung cancer (NSCLC) is the key reason for the failure of treatment, but the mechanism of this remains largely unknown. Stromal cell-derived factor 1-alpha (SDF-1α/CXCL12) is a small chemotactic cytokine protein that plays an important role in tumor progression. In this study, we investigated the anti-apoptotic mechanism of the CXCL12/CXCR4 axis in response to cisplatin, a commonly used chemotherapeutic drug, in human lung adenocarcinoma A549 cells...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28720058/long-form-of-thymic-stromal-lymphopoietin-of-keratinocytes-is-induced-by-protein-allergens
#8
Yasutaka Kuroda, Takuo Yuki, Yutaka Takahashi, Hitoshi Sakaguchi, Kayoko Matsunaga, Hiroshi Itagaki
A growing body of evidence suggests that epicutaneous sensitization of protein allergens induces immediate-type hypersensitivity (IHS) following induction of Type 2 immune responses in animals and humans. Thymic stromal lymphopoietin (TSLP) derived from keratinocytes is a cytokine that can activate dendritic cells and has been implicated in development of inflammatory Type 2 helper T-cells. However, there is no direct evidence that allergens directly regulate TSLP expression in keratinocytes. This study aimed to evaluate the response of TSLP to protein allergens in cultured human keratinocytes and to identify appropriate endpoints for IHS...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28717423/contribution-of-neuroblastoma-derived-exosomes-to-the-production-of-pro-tumorigenic-signals-by-bone-marrow-mesenchymal-stromal-cells
#9
Rie Nakata, Hiroyuki Shimada, G Esteban Fernandez, Rob Fanter, Muller Fabbri, Jemily Malvar, Pascale Zimmermann, Yves A DeClerck
The bone marrow (BM) niche is a microenvironment promoting survival, dormancy and therapeutic resistance in tumor cells. Central to this function are mesenchymal stromal cells (MSCs). Here, using neuroblastoma (NB) as a model, we demonstrate that NB cells release an extracellular vesicle (EVs) whose protein cargo is enriched in exosomal proteins but lacks cytokines and chemokines. Using three different purification methods, we then demonstrate that NB-derived exosomes were captured by MSCs and induced the production of pro-tumorigenic cytokines and chemokines, including interleukin-6 (IL-6), IL-8/CXCL8, vascular endothelial cell growth factor and monocyte-chemotactic protein-1, with exosomes prepared by size exclusion chromatography having the highest activity...
2017: Journal of Extracellular Vesicles
https://www.readbyqxmd.com/read/28715799/resident-arterial-cells-and-circulating-bone-marrow-derived-cells-both-contribute-to-intimal-hyperplasia-in-a-rat-allograft-carotid-transplantation-model
#10
Yi He, Xin Xu, Ting Zhu, Min Tang, Ju Mei, Yi Si
BACKGROUND/AIMS: Neointimal formation following vascular injury remains a major mechanism of restenosis, whereas the precise sources of neointimal cells are still uncertain. We tested the hypothesis that both injured arterial cells and non-arterial cells contribute to intimal hyperplasia. METHODS: Following allograft transplantation of the balloon-injured carotid common artery (n = 3-6), the cellular composition of the transplant grafts and the origins of neointimal cells were measured by immunohistochemistry and immunofluorescence staining...
July 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28715613/isolation-and-characterization-of-canine-placenta-derived-mesenchymal-stromal-cells-for-the-treatment-of-neurological-disorders-in-dogs
#11
Connor Long, Lee Lankford, Priyadarsini Kumar, Robert Grahn, Dori L Borjesson, Diana Farmer, Aijun Wang
Spinal cord injury (SCI) is a devastating disorder that affects humans and dogs. The prognosis of SCI depends on the severity of the injury and can include varying levels of motor and sensory deficits including devastating paraplegia and quadriplegia. Placental mesenchymal stromal cells (PMSCs) have been shown to improve wound healing and possess neuroprotective and immunomodulatory capabilities, but have not yet been clinically tested for the treatment of SCI. This study established a protocol to isolate fetal PMSCs from canine placentas and characterized their paracrine secretion profile and ability to stimulate neurons in vitro to assess their potential as a treatment option for neurological disorders in dogs...
July 17, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28714595/telocytes-in-normal-and-keratoconic-human-cornea-an-immunohistochemical-and-transmission-electron-microscopy-study
#12
Mirca Marini, Rita Mencucci, Irene Rosa, Eleonora Favuzza, Daniele Guasti, Lidia Ibba-Manneschi, Mirko Manetti
Telocytes (TC) are typically defined as cells with telopodes by their ultrastructural features. Their presence was reported in the interstitium of various organs in vertebrates, including humans. However, no study has yet described the presence of TC in the human eye and in particular, within the stromal compartment of the cornea. To address this issue, samples of normal and pathologic (keratoconic) human corneas were tested by immunohistochemistry for CD34, platelet-derived growth factor receptor α (PDGFRα) and c-kit/CD117 or examined by transmission electron microscopy...
July 17, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28713911/glucagon-like-peptide-1-analogue-liraglutide-ameliorates-atherogenesis-via-inhibiting-advanced-glycation-end-product-induced-receptor-for-advanced-glycosylation-end-product-expression-in-apolipoprotein-e-deficient-mice
#13
Peicheng Li, Zhaosheng Tang, Lin Wang, Bo Feng
Glucagon-like peptide-1 (GLP-1) can protect arteriosclerotic lesions in apolipoprotein-E deficient (ApoE-/-) mice. Advanced glycation end products (AGEs)/receptor for advanced glycation end products (RAGE) interaction serves a key role in the development of diabetic vascular complications. The present study examined whether the GLP-1 analogue liraglutide can ameliorate atherogenesis via inhibiting AGEs-induced RAGE expression. Male ApoE-/- mice (age, 10 weeks) were divided into control, GLP-1, AGEs and AGEs+GLP-1 group...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713638/neurovascular-cell-sheet-transplantation-in-a-canine-model-of-intracranial-hemorrhage
#14
Woo-Jin Lee, Jong Young Lee, Keun-Hwa Jung, Soon-Tae Lee, Hyo Yeol Kim, Dong-Kyu Park, Jung-Suk Yu, So-Yun Kim, Daejong Jeon, Manho Kim, Sang Kun Lee, Jae-Kyu Roh, Kon Chu
Cell-based therapy for intracerebral hemorrhage (ICH) has a great therapeutic potential. However, methods to effectively induce direct regeneration of the damaged neural tissue after cell transplantation have not been established, which, if done, would improve the efficacy of cell-based therapy. In this study, we aimed to develop a cell sheet with neurovasculogenic potential and evaluate its usefulness in a canine ICH model. We designed a composite cell sheet made of neural progenitors derived from human olfactory neuroepithelium and vascular progenitors from human adipose tissue-derived stromal cells...
2017: Cell Medicine
https://www.readbyqxmd.com/read/28711757/cxcr4-and-cxcr7-play-distinct-roles-in-cardiac-lineage-specification-and-pharmacologic-%C3%AE-adrenergic-response
#15
Delaine K Ceholski, Irene C Turnbull, Venu Pothula, Laura Lecce, Andrew A Jarrah, Changwon Kho, Ahyoung Lee, Lahouaria Hadri, Kevin D Costa, Roger J Hajjar, Sima T Tarzami
CXCR4 and CXCR7 are prominent G protein-coupled receptors (GPCRs) for chemokine stromal cell-derived factor-1 (SDF-1/CXCL12). This study demonstrates that CXCR4 and CXCR7 induce differential effects during cardiac lineage differentiation and β-adrenergic response in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Using lentiviral vectors to ablate CXCR4 and/or CXCR7 expression, hiPSC-CMs were tested for phenotypic and functional properties due to gene knockdown. Gene expression and flow cytometry confirmed the pluripotent and cardiomyocyte phenotype of undifferentiated and differentiated hiPSCs, respectively...
July 8, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28710661/the-promise-of-stromal-cell-derived-factor-1-in-novel-heart-disease-treatments
#16
EDITORIAL
Friedrich C Luft
No abstract text is available yet for this article.
August 2017: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/28710566/remarkable-effects-of-imatinib-in-a-family-with-young-onset-gastrointestinal-stromal-tumors-and-cutaneous-hyperpigmentation-associated-with-a-germline-kit-trp557arg-mutation-case-report-and-literature-overview
#17
S Farag, L E van der Kolk, H H van Boven, A C J van Akkooi, G L Beets, J W Wilmink, N Steeghs
Gastrointestinal stromal tumors (GISTs) occur mostly sporadically. GISTs associated with a familial syndrome are very rare and are mostly wild type for KIT and platelet-derived growth factor alpha (PDGFRA). To date 35 kindreds and 8 individuals have been described with GISTs associated with germline KIT mutations. This is the third family described with a germline p.Trp557Arg mutation in exon 11 of the KIT gene. The effect of imatinib in patients harboring a germline KIT mutation has been rarely described. Moreover, in some studies imatinib treatment was withheld considering the lack of evidence for efficacy of this treatment in GIST patients harboring a germline KIT mutation...
July 14, 2017: Familial Cancer
https://www.readbyqxmd.com/read/28710530/proteomic-characterisation-reveals-active-wnt-signalling-by-human-multipotent-stromal-cells-as-a-key-regulator-of-beta-cell-survival-and-proliferation
#18
Miljan Kuljanin, Gillian I Bell, Stephen E Sherman, Gilles A Lajoie, David A Hess
AIMS/HYPOTHESIS: Novel strategies to stimulate the expansion of beta cell mass in situ are warranted for diabetes therapy. The aim of this study was to elucidate the secretome of human bone marrow (BM)-derived multipotent stromal cells (MSCs) with documented islet regenerative paracrine function. We hypothesised that regenerative MSCs will secrete a unique combination of protein factors that augment islet regeneration. METHODS: Human BM-derived MSCs were examined for glucose-lowering capacity after transplantation into streptozotocin-treated NOD/severe combined immunodeficiency (SCID) mice and segregated into samples with regenerative (MSC(R)) vs nonregenerative (MSC(NR)) capacity...
July 14, 2017: Diabetologia
https://www.readbyqxmd.com/read/28708926/comparison-of-two-porcine-collagen-membranes-combined-with-rhbmp-2-and-rhbmp-9-on-osteoblast-behavior-in-vitro
#19
Masako Fujioka-Kobayashi, Benoit Schaler, Yoshinori Shirakata, Toshiaki Nakamura, Kazuyuki Noguchi, Yufeng Zhang, Richard J Miron
PURPOSE: To investigate the bone-inducing properties of two types of collagen membranes in combination with recombinant human bone morphogenetic protein (rhBMP)-2 and rhBMP-9 on osteoblast behavior. MATERIALS AND METHODS: Porcine pericardium collagen membranes (PPCM) and porcine dermis-derived collagen membranes (PDCM) were coated with either rhBMP-2 or rhBMP-9. The adsorption and release abilities were first investigated via enzyme-linked immunosorbent assay up to 10 days...
July 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/28706339/expansion-of-bone-marrow-derived-human-mesenchymal-stem-stromal-cells-hmscs-using-a-two-phase-liquid-liquid-system
#20
Mariana P Hanga, Halina Murasiewicz, Andrzej W Pacek, Alvin W Nienow, Karen Coopman, Christopher J Hewitt
BACKGROUND: Human mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub-cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long-term cell damage...
July 2017: Journal of Chemical Technology and Biotechnology
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