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https://www.readbyqxmd.com/read/19747855/detection-of-nanobacteria-like-material-from-calcified-cardiac-valves-with-rheumatic-heart-disease
#1
Ye-Rong Hu, Yuan Zhao, Yong-Wei Sun, Wei-Dong Lü, Zhe-Liang Liu, Jian-Ming Li, Zhong-Shi Wu, Hao Tang, Feng Gao, Xin-Min Zhou
BACKGROUND: Nanobacterium contributes to pathological calcification in human renal stones and psammoma bodies in ovarian cancer. Pathological calcification is also present in cardiac valves with rheumatic heart disease. The aim of this study was to detect, isolate, culture, and characterize nanobacteria-like material from human calcified cardiac valves with rheumatic heart disease. METHODS: Normal and calcified cardiac valve groups, as well as positive (nanobacteria strain Se90) and negative (serum radiated with 30 kGy of γ-ray) control groups, were included in this study...
September 2010: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/17587506/is-there-any-relation-of-nanobacteria-with-periodontal-diseases
#2
Turgut Demir
Periodontal diseases, including gingivitis and periodontitis, have been described as inflammation of the supporting tissues of the teeth. The main cause of periodontal disease is dental plaque. If dental plaque is not eliminated of dental surface, mineralized dental plaque (calculus) occur. The mineralization process of calculus is similar to that of other ectopic calcifications, such as kidney stones and gallstones. The presence of a certain type of microorganism discovered during the last decade in various pathogenic calcification such as renal stones, atherosclerotic plaques...
2008: Medical Hypotheses
https://www.readbyqxmd.com/read/16122881/-nanobacterium-sanguineum-is-it-a-new-life-form-in-search-of-human-ailment-or-commensal-overview-of-its-transmissibility-and-chemical-means-of-intervention
#3
Bhupat D Rawal, Anne-Marié Pretorius
Morphological, cultural, and immuno-histochemical characteristics of "Nanobacterium sanguineum" (NB) described in the literature are reviewed. NB is reported to be a motile, Gram negative organism that divides by binary fission within a calcium-coated slimy shell; this yeast-like shell replicates by budding. It measures between 20 and 200 nm with a unique structure containing 16S ribosomal RNA. NB has been observed by electron microscopy in coronary artery plaques (CAD) and in kidney stones (KS) found in renal diseases...
2005: Medical Hypotheses
https://www.readbyqxmd.com/read/14982274/nanobacteria-caused-mitral-valve-calciphylaxis-in-a-man-with-diabetic-renal-failure
#4
Tomislav M Jelic, Amer M Malas, Samuel S Groves, Bo Jin, Paul F Mellen, Garry Osborne, Rod Roque, James G Rosencrance, Ho-Huang Chang
We have found that nanobacteria, recently discovered Gram-negative atypical bacteria, can cause local calciphylaxis on the mitral valve in a setting of high-calcium X phosphorous product in the blood. We present the case of a 33-year-old man with diabetic renal failure on continuous ambulatory peritoneal dialysis who died as a result of multiple brain infarcts due to embolizations from mitral valve vegetations. Systemic calciphylaxis was not present. Spectrometric analysis of the mitral valve vegetations showed that they were composed of calcium phosphate, carbonate apatite form, and fibrin...
February 2004: Southern Medical Journal
https://www.readbyqxmd.com/read/12517874/attempted-isolation-of-nanobacterium-sp-microorganisms-from-upper-urinary-tract-stones
#5
Michel Drancourt, Véronique Jacomo, Hubert Lépidi, Eric Lechevallier, Vincent Grisoni, Christian Coulange, Edith Ragni, Claude Alasia, Bertrand Dussol, Yvon Berland, Didier Raoult
A single team has reported isolation of nanobacteria in human and bovine blood products, as well as, more recently, kidney stones. This has raised controversy. To confirm the data, we searched for nanobacteria from 10 aseptically removed upper urinary tract (UUT) stones. We used scanning electronic microscopy (SEM) with four stones and culture of stones on either 3T6 fibroblast monolayers or liquid RPMI medium. Detection of nanobacteria was made with a commercially available monoclonal antibody, 16S ribosomal DNA amplification with specific primers, and transmission electronic microscopy (TEM) of inoculated cells...
January 2003: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/11230398/infection-with-bartonella-weissii-and-detection-of-nanobacterium-antigens-in-a-north-carolina-beef-herd
#6
E B Breitschwerdt, S Sontakke, A Cannedy, S I Hancock, J M Bradley
Very recently, Bartonella organisms have been isolated from large ruminants (deer, elk, and dairy and beef cattle) located in the United States and in France. In this study, we report the serologic, microbiologic, and molecular findings related to the isolation of a Bartonella species in North Carolina beef cattle and the detection of nanobacterial antigen using a commercially available enzyme-linked immunosorbent assay. Between August 1998 and September 1999, blood was collected from 38 cattle ranging in age from 1 month to 6...
March 2001: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/11027350/an-alternative-interpretation-of-nanobacteria-induced-biomineralization
#7
J O Cisar, D Q Xu, J Thompson, W Swaim, L Hu, D J Kopecko
The reported isolation of nanobacteria from human kidney stones raises the intriguing possibility that these microorganisms are etiological agents of pathological extraskeletal calcification [Kajander, E. O. & Ciftçioglu, N. (1998) Proc. Natl. Acad. Sci. USA 95, 8274-8279]. Nanobacteria were previously isolated from FBS after prolonged incubation in DMEM. These bacteria initiated biomineralization of the culture medium and were identified in calcified particles and biofilms by nucleic acid stains, 16S rDNA sequencing, electron microscopy, and the demonstration of a transferable biomineralization activity...
October 10, 2000: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/10844606/endotoxin-and-nanobacteria-in-polycystic-kidney-disease
#8
J T Hjelle, M A Miller-Hjelle, I R Poxton, E O Kajander, N Ciftcioglu, M L Jones, R C Caughey, R Brown, P D Millikin, F S Darras
BACKGROUND: Microbes have been suspected as provocateurs of polycystic kidney disease (PKD), but attempts to isolate viable organisms have failed. Bacterial endotoxin is the most often reported microbial product found in PKD fluids. We assessed potential microbial origins of endotoxin in cyst fluids from 13 PKD patients and urines of PKD and control individuals. METHODS: Fluids were probed for endotoxin and nanobacteria, a new bacterium, by the differential Limulus Amebocyte Lysate assay (dLAL), genus-specific antilipopolysaccharide (LPS) antibodies, monoclonal antibodies to nanobacteria, and hyperimmune serum to Bartonella henselae (HS-Bh)...
June 2000: Kidney International
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