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https://www.readbyqxmd.com/read/28609472/no-evidence-for-genome-editing-in-mouse-zygotes-and-hek293t-human-cell-line-using-the-dna-guided-natronobacterium-gregoryi-argonaute-ngago
#1
Nay Chi Khin, Jenna L Lowe, Lora M Jensen, Gaetan Burgio
A recently published research article reported that the extreme halophile archaebacterium Natronobacterium gregoryi Argonaute enzyme (NgAgo) could cleave the cellular DNA under physiological temperature conditions in cell line and be implemented as an alternative to CRISPR/Cas9 genome editing technology. We assessed this claim in mouse zygotes for four loci (Sptb, Tet-1, Tet-2 and Tet-3) and in the human HEK293T cell line for the EMX1 locus. Over 100 zygotes were microinjected with nls-NgAgo-GK plasmid provided from Addgene and various concentrations of 5'-phosphorylated guide DNA (gDNA) from 2...
2017: PloS One
https://www.readbyqxmd.com/read/28494027/no-evidence-of-genome-editing-activity-from-natronobacterium-gregoryi-argonaute-ngago-in-human-cells
#2
Parisa Javidi-Parsijani, Guoguang Niu, Meghan Davis, Pin Lu, Anthony Atala, Baisong Lu
The argonaute protein from the thermophilic bacterium Thermus thermophilus shows DNA-guided DNA interfering activity at high temperatures, complicating its application in mammalian cells. A recent work reported that the argonaute protein from Natronobacterium gregoryi (NgAgo) had DNA-guided genome editing activity in mammalian cells. We compared the genome editing activities of NgAgo and Staphylococcus aureus Cas9 (SaCas9) in human HEK293T cells side by side. EGFP reporter assays and DNA sequencing consistently revealed high genome editing activity from SaCas9...
2017: PloS One
https://www.readbyqxmd.com/read/28128770/modern-genome-editing-technologies-in-huntington-s-disease-research
#3
Tuyana B Malankhanova, Anastasia A Malakhova, Sergey P Medvedev, Suren M Zakian
The development of new revolutionary technologies for directed gene editing has made it possible to thoroughly model and study NgAgo human diseases at the cellular and molecular levels. Gene editing tools like ZFN, TALEN, CRISPR-based systems, NgAgo and SGN can introduce different modifications. In gene sequences and regulate gene expression in different types of cells including induced pluripotent stem cells (iPSCs). These tools can be successfully used for Huntington's disease (HD) modeling, for example, to generate isogenic cell lines bearing different numbers of CAG repeats or to correct the mutation causing the disease...
2017: Journal of Huntington's Disease
https://www.readbyqxmd.com/read/28066798/an-ngago-tool-for-genome-editing-did-crispr-cas9-just-find-a-competitor
#4
Qiang Wei, Junyi Liao, Xinyi Yu, Eric J Wang, Claire Wang, Hue H Luu, Rex C Haydon, Michael J Lee, Tong-Chuan He
While CRISPR/Cas9-mediated genome editing technology has been experiencing a rapid transformation during the past few years, a recent report on NgAgo-mediated single-stranded DNA-guided genome editing may offer an attractive alternative for genome manipulation. While it's too early to predict whether NgAgo will be able to compete with or be superior to CRISPR/Cas9, the scientific community is anxiously waiting for further optimization and broader applications of the NgAgo genome editing technology.
September 2016: Genes & Diseases
https://www.readbyqxmd.com/read/27913985/erratum-to-questions-about-ngago
#5
Shawn Burgess, Linzhao Cheng, Feng Gu, Junjiu Huang, Zhiwei Huang, Shuo Lin, Jinsong Li, Wei Li, Wei Qin, Yujie Sun, Zhou Songyang, Wensheng Wei, Qiang Wu, Haoyi Wang, Xiaoqun Wang, Jing-Wei Xiong, Jianzhong Xi, Hui Yang, Bin Zhou, Bo Zhang
No abstract text is available yet for this article.
January 2017: Protein & Cell
https://www.readbyqxmd.com/read/27905463/updated-ngago-gene-editing-controversy-escalates-in-peer-reviewed-papers
#6
COMMENT
David Cyranoski
No abstract text is available yet for this article.
November 23, 2016: Nature
https://www.readbyqxmd.com/read/27858349/ngago-a-hope-or-a-hype
#7
EDITORIAL
Xiaoxue Zhang
No abstract text is available yet for this article.
December 2016: Protein & Cell
https://www.readbyqxmd.com/read/27834346/ngago-based-fabp11a-gene-knockdown-causes-eye-developmental-defects-in-zebrafish
#8
Jialing Qi, Zhangji Dong, Yunwei Shi, Xin Wang, Yinyin Qin, Yongming Wang, Dong Liu
No abstract text is available yet for this article.
December 2016: Cell Research
https://www.readbyqxmd.com/read/27725818/survival-and-evolution-of-crispr-cas-system-in-prokaryotes-and-its-applications
#9
Muhammad Abu Bakr Shabbir, Haihong Hao, Muhammad Zubair Shabbir, Hafiz Iftikhar Hussain, Zahid Iqbal, Saeed Ahmed, Adeel Sattar, Mujahid Iqbal, Jun Li, Zonghui Yuan
Prokaryotes have developed numerous innate immune mechanisms in order to fend off bacteriophage or plasmid attack. One of these immune systems is clustered regularly interspaced short palindromic repeats (CRISPR). CRISPR-associated proteins play a key role in survival of prokaryotes against invaders, as these systems cleave DNA of foreign genetic elements. Beyond providing immunity, these systems have significant impact in altering the bacterial physiology in term of its virulence and pathogenicity, as well as evolution...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27712579/to-edit-or-not-the-ngago-story
#10
Nathan Blow
New genome-editing approaches always receive widespread attention. But in the case of a novel Argonaute-based technique published last spring, attention has been particularly intense.
2016: BioTechniques
https://www.readbyqxmd.com/read/27510204/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies
#11
David Cyranoski
No abstract text is available yet for this article.
August 11, 2016: Nature
https://www.readbyqxmd.com/read/27230540/ssdna-and-the-argonautes-the-quest-for-the-next-golden-editor
#12
Gabriel Martínez-Gálvez, Hirotaka Ata, Jarryd M Campbell, Stephen C Ekker
Genome engineering has gone mainstream because of breakthroughs in defining and harnessing naturally occurring, customizable DNA recognition cursors (protein or RNA-guided). At present, most gene editing relies on these cursors to direct custom DNA endonucleases to a specific genomic sequence to induce a double-strand break. New tools for genome engineering are continuously being explored, and another advance in DNA targeting has recently been described. Argonaute isolated from Natronobacterium gregoryi (NgAgo) is an ssDNA-based cursor that thus far has no known limitations in sequence recognition, shows promise for high specificity, and for many applications may represent a potentially more accessible genome-editing system over prior tools as it requires only a single, 24-base, 5' phosphorylated ssDNA for DNA targeting...
June 2016: Human Gene Therapy
https://www.readbyqxmd.com/read/27136078/dna-guided-genome-editing-using-the-natronobacterium-gregoryi-argonaute
#13
Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu, Chunyu Han
The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ∼24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA...
July 2016: Nature Biotechnology
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