Yeh-Hsing Lao, Mingqiang Li, Madeleine A Gao, Dan Shao, Chun-Wei Chi, Dantong Huang, Syandan Chakraborty, Tzu-Chieh Ho, Weiqian Jiang, Hong-Xia Wang, Sihong Wang, Kam W Leong
CRISPR/Cas9 technology enables targeted gene editing; yet, the efficiency and specificity remain unsatisfactory, particularly for the nonvirally delivered, plasmid-based CRISPR/Cas9 system. To tackle this, a self-assembled micelle is developed and evaluated for human papillomavirus (HPV) E7 oncogene disruption. The optimized micelle enables effective delivery of Cas9 plasmid with a transient transgene expression profile, benefiting the specificity of Cas9 recognition. Furthermore, the feasibility of using the micelle is explored for another nucleic acid-guided nuclease system, Natronobacterium gregoryi Argonaute (NgAgo)...
July 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)