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OXA-48

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https://www.readbyqxmd.com/read/27902381/detection-of-escherichia-coli-st131-complex-st705-and-klebsiella-pneumoniae-st15-among-fecal-carriage-of-extended-spectrum-betalactamase-and-carbapenemase-producing-enterobacteriaceae
#1
Esther Rios, Maria Carmen López, Iciar Rodríguez-Avial, Esther Culebras, Juan Jose Picazo
The objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and carbapenemases-producing Enterobacteriaceae (CPE) in non-selected hospitalized and non-hospitalized patients from the same geographic area of Madrid. Among the 501 patients enrolled, 43 (8.6%) carried ESBL-E and 8 (1.6%) patients exhibited CPE. The main intestinal colonizer among ESBL-E was CTX-M-producing Escherichia coli isolates in both settings (community and hospital)...
November 28, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27897435/-evaluation-of-an-immunochromatographic-test-for-the-detection-of-oxa-48-carbapenemase
#2
C Mediavilla-Gradolph, R Sáinz-Rodriguez, M Valverde-Troya, I de Toro-Peinado, M P Bermudez-Ruíz, B Palop-Borrás
OBJECTIVE: Detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. OXA-48 is the most common carbapenemase in Spain and in our environment. The aim of this study is the evaluation of a new immunochromatographic test OXA-48 Card letitest (Coris, BioConcept Belgium) to detect this carbapenemase from solid media. METHODS: During the last year 151 strains of carbapenemase producing bacteria have been isolated, of which 136 were OXA-48 (126 Klebsiella pneumoniae, 1 Klebsiella oxytoca, 5 Escherichia coli, 4 Enterobacter cloacae), and 15 producing other carbapenemases ...
November 25, 2016: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/27890667/investigation-of-carbapenem-resistance-and-the-first-identification-of-klebsiella-pneumoniae-carbapenemase-kpc-enzyme-among-escherichia-coli-isolates-in-turkey-a-prospective-study
#3
Mert Ahmet Kuskucu, Asiye Karakullukcu, Mailihaba Ailiken, Barıs Otlu, Bilgul Mete, Gokhan Aygun
BACKGROUND: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. METHODS: The prospective study included a total of 4052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively...
November 23, 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27866944/occurrence-of-carbapenemase-producing-klebsiella-pneumoniae-and-escherichia-coli-in-the-european-survey-of-carbapenemase-producing-enterobacteriaceae-euscape-a-prospective-multinational-study
#4
Hajo Grundmann, Corinna Glasner, Barbara Albiger, David M Aanensen, Chris T Tomlinson, Arjana Tambić Andrasević, Rafael Cantón, Yehuda Carmeli, Alexander W Friedrich, Christian G Giske, Youri Glupczynski, Marek Gniadkowski, David M Livermore, Patrice Nordmann, Laurent Poirel, Gian M Rossolini, Harald Seifert, Alkiviadis Vatopoulos, Timothy Walsh, Neil Woodford, Dominique L Monnet
BACKGROUND: Gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in Europe make it difficult to contain carbapenemase-producing Enterobacteriaceae. We report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in European hospitals. METHODS: National expert laboratories recruited hospitals with diagnostic capacities, who collected the first ten carbapenem non-susceptible clinical isolates of K pneumoniae or E coli and ten susceptible same-species comparator isolates and pertinent patient and hospital information...
November 17, 2016: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/27866753/-molecular-epidemiology-of-carbapenemase-producing-enterobacteriaceae-infection-colonisation-in-a-hospital-in-madrid
#5
Patricia Brañas, Marta Gil, Jennifer Villa, María Ángeles Orellana, Fernando Chaves
INTRODUCTION: A description is presented on the molecular epidemiology of carbapenemase-producing enterobacteriaceae infection in a tertiary hospital. MATERIAL AND METHODS: A study was made on all the carbapenemase-producing enterobacteriaceae isolations obtained between February 2015 and March 2016 in the Hospital Universitario 12 de Octubre (Madrid). Phenotypic and molecular methods were used. RESULTS: A total of 7 bacterial species were identified, with the majority being Klebsiella pneumoniae (K...
November 17, 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/27863949/imipenem-avibactam-a-novel-combination-for-the-rapid-detection-of-carbapenemase-activity-in-enterobacteriaceae-and-acinetobacter-baumannii-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#6
Marina Oviaño, Germán Bou
In the present study, we propose a novel matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for detecting carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii. For this, we analyzed a series of 131 isolates. Among them, a total of 115 Enterobacteriaceae: 79 of them carrying a carbapenemase enzyme (15blaKPC, 7blaNDM, 11blaIMP, 12blaVIM, and 34blaOXA-48) and 16 A. baumannii isolates: 15 of them carrying carbapenemases (10blaOXA-23, 2blaOXA-58, 2blaOXA-24, and 1blaOXA-237)...
October 18, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27855076/molecular-characterization-of-oxa-48-like-producing-enterobacteriaceae-in-the-czech-republic-evidence-for-horizontal-transfer-of-poxa-48-like-plasmids
#7
Anna Skálová, Kateřina Chudějová, Veronika Rotová, Matej Medvecky, Vendula Študentová, Eva Chudáčková, Pavel Lavicka, Tamara Bergerova, Vladislav Jakubu, Helena Zemlickova, Costas C Papagiannitsis, Jaroslav Hrabák
The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings, in the Czech Republic. During 2013-2015, twenty-two Klebsiella pneumoniae, three Escherichia coli and one Enterobacter cloacae producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized/infected by two or three different OXA-48-like producers. K. pneumoniae isolates were classified into nine STs, with ST101 being the predominant (n=8)...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27842873/in-vitro-activities-of-colistin-tigecycline-and-tobramycin-alone-or-in-combination-against-carbapenem-resistant-enterobacteriaceae-strains
#8
Berna Ozbek, Emel Mataracı-Kara, Sevda Er, Melda Ozdamar, Mesut Yilmaz
The aim of this study was to investigate the activities of various antibiotics, alone or in combination, against carbapenem-resistant Enterobacteriaceae (CRE). Minimum inhibitory concentrations (MICs) of meropenem, colistin, tigecycline and tobramycin were determined by microbroth dilution against 40 clinical strains. Carbapenemase-encoding genes were detected by PCR using specific primers. The in vitro synergistic activities of tigecycline, colistin and tobramycin in double antibiotic combinations were determined by the microbroth chequerboard technique, and results were interpreted using the fractional inhibitory concentration index (FICI)...
December 2015: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27802107/clonal-spread-of-colistin-resistant-klebsiella-pneumoniae-coproducing-kpc-and-vim-carbapenemases-in-neonates-at-a-tunisian-university-hospital
#9
Hajer Battikh, Chiraz Harchay, Amal Dekhili, Khaoula Khazar, Fehima Kechrid, Meriem Zribi, Afef Masmoudi, Chedlia Fendri
In this study, we have attempted to report the first clonal spread of colistin-resistant Klebsiella pneumoniae coproducing KPC and VIM carbapenemases in the neonatal unit of Rabta Teaching Hospital of Tunis (Tunisia). This retrospective study was performed from January 1, 2014 to December 31, 2014 in the Microbiology Laboratory at the Rabta University Hospital of Tunis. Twenty-one nonreplicate colistin-resistant K. pneumoniae were isolated from 19 patients hospitalized in the neonatal unit and 2 patients in the adult intensive care unit (ICU)...
November 1, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27795386/evaluation-of-the-carba-np-test-in-oregon-2013
#10
Karim E Morey, Robert Vega, P Maureen Cassidy, Genevieve L Buser, Jaipreet K Rayar, Jeffrey A Myers, Scott J Weissman, Zintars G Beldavs, Christopher D Pfeiffer
Carbapenem-resistant Enterobacteriaceae (CRE) are an urgent public health threat. We evaluated the capacity of the Carba NP test to detect carbapenemase production in 206 isolates: 143 Enterobacteriaceae identified by Oregon's CRE surveillance in 2013 and 63 known carbapenemase-positive organisms. Overall, test sensitivity and specificity were 89% (59/66; 95% CI 81-97%) and 100% (140/140; 95% CI 98-100%), respectively. All KPC, NDM-1, VIM, and IMP-producers but no (0/7) OXA-48-like strains were Carba NP-positive prior to a post-hoc protocol modification...
October 17, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27794265/molecular-epidemiology-of-environmental-and-clinical-carbapenemase-producing-gram-negative-bacilli-from-hospitals-in-guelma-algeria-multiple-genetic-lineages-and-first-report-of-oxa-48-in-enterobacter-cloacae
#11
Widad Bouguenoun, Sofiane Bakour, Ahmed Aimen Bentorki, Charbel Al Bayssari, Tarek Merad, Jean-Marc Rolain
This study was designed to investigate environmental colonisation in Algerian hospitals by carbapenem-resistant Gram-negative bacilli (GNB), including molecular characterisation of their resistance, and to perform a comparative molecular analysis between clinical and environmental strains. GNB isolated from hospitalised patients and the hospital environment were identified using microbiological methods and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was performed by disk diffusion and Etest methods...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27793962/incfiik-plasmid-harbouring-an-amplification-of-16s-rrna-methyltransferase-encoding-gene-rmth-associated-with-mobile-element-iscr2
#12
Racha Beyrouthy, Frederic Robin, Monzer Hamze, Richard Bonnet
OBJECTIVES: To investigate the resistance mechanisms and genetic support underlying the high resistance level of the Klebsiella pneumoniae strain CMUL78 to aminoglycoside and β-lactam antibiotics. METHODS: Antibiotic susceptibility was assessed by the disc diffusion method and MICs were determined by the microdilution method. Antibiotic resistance genes and their genetic environment were characterized by PCR and Sanger sequencing. Plasmid contents were analysed in the clinical strain and transconjugants obtained by mating-out assays...
October 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27760718/laboratory-detection-of-intestinal-carriage-of-carbapenemase-producing-enterobacteriaceae-a-comparison-of-algorithms-using-the-carba-np-test
#13
James Knox, Claire Gregory, Louise Prendergast, Chandrika Perera, Jennifer Robson, Lynette Waring
Stool specimens spiked with a panel of 46 carbapenemase-producing Enterobacteriaceae (CPE) and 59 non-carbapenemase producers were used to compare the diagnostic accuracy of 4 testing algorithms for the detection of intestinal carriage of CPE: (1) culture on Brilliance ESBL agar followed by the Carba NP test; (2) Brilliance ESBL followed by the Carba NP test, plus chromID OXA-48 agar with no Carba NP test; (3) chromID CARBA agar followed by the Carba NP test; (4) chromID CARBA followed by the Carba NP test, plus chromID OXA-48 with no Carba NP test...
September 30, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27754776/first-description-of-kpc-2-producing-escherichia-coli-and-st15-oxa-48-positive-klebsiella-pneumoniae-in-tunisia
#14
Farah Ben Tanfous, Carla Andrea Alonso, Wafa Achour, Laura Ruiz-Ripa, Carmen Torres, Assia Ben Hassen
The aim of this study was to investigate the molecular features among Klebsiella pneumoniae and Escherichia coli strains showing a resistant/intermediate-resistant phenotype to ertapenem (R/IR-ERT), implicated in colonization/infection in patients of the Hematology and Graft Units of the National Bone Marrow Transplant Center of Tunisia (3-year period, 2011-2014). The major carbapenemase, extended-spectrum beta-lactamase, and plasmidic AmpC beta-lactamase genes were analyzed and characterized by PCR and sequencing...
October 18, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27747206/current-state-of-resistance-to-antibiotics-of-last-resort-in-south-africa-a-review-from-a-public-health-perspective
#15
John Osei Sekyere
A review of the literature was undertaken to delineate the current level and mechanisms of resistance to carbapenems, colistin, and tigecycline in South Africa. Thirty-two English publications and 32 National Institute of Communicable Diseases communiqués identified between early January 2000 and 20 May, 2016 showed substantial reports of NDM (n = 860), OXA-48 (n = 584), VIM (n = 131), and IMP (n = 45) carbapenemases within this period, mainly in Klebsiella pneumoniae (n = 1138), Acinetobacter baumannii (n = 332), Enterobacter cloacae (n = 201), and Serratia marcescens (n = 108)...
2016: Frontiers in Public Health
https://www.readbyqxmd.com/read/27737785/detection-of-carbapenemase-activity-in-enterobacteriaceae-using-lc-ms-ms-in-comparison-with-the-neo-rapid-carb-kit-using-direct-visual-assessment-and-colorimetry
#16
Charlotte A Huber, Hanna E Sidjabat, Hosam M Zowawi, David Kvaskoff, Sarah Reed, John F McNamara, Kate L McCarthy, Patrick Harris, Benjamin Toh, Alexander M Wailan, David L Paterson
It has been described that the sensitivity of the Carba NP test may be low in the case of OXA-48-like carbapenamases and mass spectrometry based methods as well as a colorimetry based method have been described as alternatives. We evaluated 84 Enterobacteriaceae isolates including 31 OXA-48-like producing isolates and 13 isolates that produced either an imipenemase (IMP; n=8), New Delhi metallo-β-lactamase (NDM; n=3), or Klebsiella pneumoniae carbapenemase (KPC; n=2), as well as 40 carbapenemase negative Enterobacteriaceae isolates...
October 11, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27716102/antimicrobial-susceptibility-testing-in-predicting-the-presence-of-carbapenemase-genes-in-enterobacteriaceae-in-south-africa
#17
Ashika Singh-Moodley, Olga Perovic
BACKGROUND: Carbapenem-resistant Enterobacteriaceae (CRE) is a concern in South Africa and worldwide. It is therefore important that these organisms be accurately identified for infection prevention control purposes. METHOD: In this study 1193 suspected CREs from 46 laboratories from seven provinces in South Africa were assessed to confirm the prevalence of carbapenemase genes from our referral diagnostic isolates for the period 2012 to 2015. We compared the antimicrobial susceptibility testing method used in the reference laboratory to the polymerase chain reaction (PCR) which is used as the gold standard...
October 4, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27685838/national-survey-of-colistin-resistance-among-carbapenemase-producing-enterobacteriaceae-and-outbreak-caused-by-colistin-resistant-oxa-48-producing-klebsiella-pneumoniae-france-2014
#18
Aurélie Jayol, Laurent Poirel, Laurent Dortet, Patrice Nordmann
From January 2014 to December 2014, 972 consecutive non-replicate carbapenemase-producing Enterobacteriaceae isolates from colonised or infected patients were collected at the Associated French National Reference Centre as part of the French national survey on antimicrobial resistance. It included 577 Klebsiella spp. (59%), 236 Escherichia coli (24%), 108 Enterobacter spp. (11%), 50 Citrobacter spp. (5%), and a single Salmonella spp. isolate (0.1%). Of 561 K. pneumoniae isolates, 35 were found to be resistant to colistin (6...
September 15, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/27645609/development-and-evaluation-of-a-multiplex-real-time-pcr-for-the-detection-of-imp-vim-and-oxa-23-carbapenemase-gene-families-on-the-bd-max-open-system
#19
Jeanette W P Teo, My-Van La, Raymond T P Lin
A multiplex real-time polymerase chain reaction (PCR) assay was developed for the detection of clinically prevalent IMP, VIM, and OXA-23 gene families. The assay was designed to work on the BD MAX open platform which is a fully automated system for all PCR processes including sample extraction to PCR resulting. A total of 107 well-characterized carbapenem resistant Enterobacteriaceae were evaluated and the results were 100% concordant with the reference test isolates. This assay will serve to complement PCR screens that detect the major carbapenemase families of NDM, KPC, and OXA-48-like...
August 26, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27645236/outbreak-of-oxa-48-producing-klebsiella-pneumoniae-involving-an-st-101-clone-in-batna-university-hospital-algeria
#20
Lotfi Loucif, Ahmed Kassah Laouar, Mahdia Saidi, Amina Messala, Widad Chelaghma, Jean-Marc Rolain
Seven non-redundant ertapenem-resistant Klebsiella pneumoniae isolates were collected from May 2014 to January 19, 2015 in the nephrology and hematology units of Batna University Hospital, Algeria. All strains coproduced the blaOXA-48, blaCTX-M-15, blaSHV-1 and blaTEM-1D genes. Six of these isolates belonged to the pandemic clone ST101. The blaOXA-48 gene was located on a conjugative IncL/M-type plasmid. This is the first outbreak of OXA-48-producing K. pneumoniae isolates involving an ST101 clone in Batna University Hospital, Algeria...
September 19, 2016: Antimicrobial Agents and Chemotherapy
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