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OXA-48

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https://www.readbyqxmd.com/read/28080212/heterogeneity-of-carbapenem-resistance-mechanisms-among-gram-negative-pathogens-in-lebanon-results-of-the-first-cross-sectional-countrywide-study
#1
Dalal Hammoudi Halat, Carole Ayoub Moubareck, Dolla Karam Sarkis
Carbapenem-resistant Gram-negative pathogens have progressively disseminated to different countries worldwide, presenting a serious public health concern. The aims of this study were to determine the prevalence of carbapenem resistance in Gram-negative bacteria in Lebanon, to elucidate molecular mechanisms, and to identify genetic relatedness of incriminated strains. Carbapenem nonsusceptible Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas were collected from 11 Lebanese hospitals in 2012. Antimicrobial susceptibility was assessed with phenotypic tests, genes encoding carbapenemases were screened via PCR-sequencing, and genetic relatedness was examined by PGFE and ERIC-PCR...
January 12, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28039279/oxa-48-producing-st372-escherichia-coli-in-a-french-dog
#2
Luana C Melo, Marine N G Boisson, Estelle Saras, Christine Médaille, Henri-Jean Boulouis, Jean-Yves Madec, Marisa Haenni
No abstract text is available yet for this article.
December 30, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28031433/comparison-of-phenotypic-tests-and-an-immunochromatographic-assay-and-development-of-a-new-algorithm-for-oxa-48-like-detection
#3
Florian Koroska, Stephan Göttig, Martin Kaase, Jörg Steinmann, Sören Gatermann, Julian Sommer, Thorsten Wille, Georg Plum, Axel Hamprecht
OXA-48 is the most prevalent carbapenemase in Enterobacteriaceae in Europe and the Middle East, but is frequently missed because many isolates display low minimal inhibitory concentrations (MIC) for carbapenems. Furthermore, in contrast to metallo-β-lactamases or Klebsiella pneumoniae carbapenemases (KPC), no specific inhibitor is available for the phenotypic detection of OXA-48. Molecular detection of blaOXA-48 is the gold standard, but is not available in many laboratories. Few phenotypic assays have been described, but have not been independently evaluated...
December 28, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28013264/comparing-the-outcomes-of-patients-with-carbapenemase-producing-and-non-carbapenemase-producing-carbapenem-resistant-enterobacteriaceae-bacteremia
#4
Pranita D Tamma, Katherine E Goodman, Anthony D Harris, Tsigereda Tekle, Ava Roberts, Abimbola Taiwo, Patricia J Simner
BACKGROUND: Carbapenem-resistant Enterobacteriaceae (CRE) are associated with considerable mortality. As mechanisms of carbapenem resistance are heterogeneous, it is unclear if mortality differs based on resistance mechanisms. We sought to determine whether CRE resistance mechanism determination is prognostically informative. METHODS: We conducted an observational study comparing 14-day mortality between patients with carbapenemase-producing (CP)-CRE compared with non-CP-CRE bacteremia...
November 9, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/27988171/evaluation-of-a-rapid-immunochromatographic-test-for-the-detection-of-oxa-48-carbapenemase
#5
Elisa Rubio, Yuliya Zboromyrska, Cristina Pitart, Irene Campo, Izaskun Alejo-Cancho, Assumpta Fasanella, Andrea Vergara, Francesc Marco, Jordi Vila
We evaluated the OXA-48K-Set, a rapid immunochromatographic test for the detection of Oxacillinase-48 (OXA-48) carbapenemases, among 37 strains expressing OXA-48 and OXA-48-like carbapenemases and 20 additional strains harboring other β-lactamases. The test showed 100% sensitivity and specificity and the results were obtained in 15minutes.
December 6, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27965626/high-prevalence-of-gut-microbiota-colonization-with-broad-spectrum-cephalosporin-resistant-enterobacteriaceae-in-a-tunisian-intensive-care-unit
#6
Elaa Maamar, Sana Ferjani, Ali Jendoubi, Samia Hammami, Zaineb Hamzaoui, Laure Mayonnove-Coulange, Mabrouka Saidani, Aouatef Kammoun, Amel Rehaiem, Salma Ghedira, Mohamed Houissa, Ilhem Boutiba-Ben Boubaker, Amine Slim, Veronique Dubois
Healthcare-associated infections due to cefotaxime-resistant (CTX-R) Enterobacteriaceae have become a major public health threat, especially in intensive care units (ICUs). Often acquired nosocomially, CTX-R Enterobacteriaceae can be introduced initially by patients at admission. This study aimed to determine the prevalence and genetic characteristics of CTX-R Enterobacteriaceae-intestinal carriage in ICU patients, to evaluate the rate of acquisition of these organisms during hospitalization, and to explore some of the associated risk factors for both carriage and acquisition...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27927915/comparative-evaluation-of-four-phenotypic-tests-for-the-detection-of-carbapenemase-producing-gram-negative-bacteria
#7
Audrey Noël, Te-Din Huang, Catherine Berhin, Martin Hoebeke, Warda Bouchahrouf, Sami Yunus, Pierre Bogaerts, Youri Glupczynski
Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates i.e. the Neo-Rapid CARB kit (Rosco Diagnostica A/S), the RAPIDEC CARBA NP test (BioMérieux SA), the β CARBA test (Bio-Rad Laboratories N.V) and a home-made electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates (Enterobacteriaceae [n= 198], nonfermentative Gram-negative bacilli [n = 130]) with previously characterized resistance mechanisms to carbapenems...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27916545/chromagar-msupercarba-performance-in-carbapenem-resistant-enterobacteriaceae-isolates-characterized-at-molecular-level-and-routine-surveillance-rectal-swab-specimens
#8
Sergio García-Fernández, Marta Hernández-García, Aránzazu Valverde, Patricia Ruiz-Garbajosa, María Isabel Morosini, Rafael Cantón
Performance of the CHROMagar mSuperCARBA media was assessed in both well-characterized carbapenem-resistant Enterobacteriaceae (n=52) and routine surveillance rectal swab specimens (n=211). Limit of detection ranged between 10(1) and 10(2)CFU/mL except for OXA-48 producers with low-carbapenem MICs (10(6)CFU/mL). High sensitivity (100%) and specificity (100%) were obtained with rectal swabs.
November 25, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27902381/detection-of-escherichia-coli-st131-complex-st705-and-klebsiella-pneumoniae-st15-among-fecal-carriage-of-extended-spectrum-betalactamase-and-carbapenemase-producing-enterobacteriaceae
#9
Esther Rios, Maria Carmen López, Iciar Rodríguez-Avial, Esther Culebras, Juan Jose Picazo
The objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) and carbapenemases-producing Enterobacteriaceae (CPE) in non-selected hospitalized and non-hospitalized patients from the same geographic area of Madrid. Among the 501 patients enrolled, 43 (8.6%) carried ESBL-E and 8 (1.6%) patients exhibited CPE. The main intestinal colonizer among ESBL-E was CTX-M-producing Escherichia coli isolates in both settings (community and hospital)...
November 28, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27897435/-evaluation-of-an-immunochromatographic-test-for-the-detection-of-oxa-48-carbapenemase
#10
C Mediavilla-Gradolph, R Sáinz-Rodriguez, M Valverde-Troya, I de Toro-Peinado, M P Bermudez-Ruíz, B Palop-Borrás
OBJECTIVE: Detection and differentiation of various types of carbapenemases is crucial to their control and dissemination. OXA-48 is the most common carbapenemase in Spain and in our environment. The aim of this study is the evaluation of a new immunochromatographic test OXA-48 Card letitest (Coris, BioConcept Belgium) to detect this carbapenemase from solid media. METHODS: During the last year 151 strains of carbapenemase producing bacteria have been isolated, of which 136 were OXA-48 (126 Klebsiella pneumoniae, 1 Klebsiella oxytoca, 5 Escherichia coli, 4 Enterobacter cloacae), and 15 producing other carbapenemases ...
November 25, 2016: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/27890667/investigation-of-carbapenem-resistance-and-the-first-identification-of-klebsiella-pneumoniae-carbapenemase-kpc-enzyme-among-escherichia-coli-isolates-in-turkey-a-prospective-study
#11
Mert Ahmet Kuskucu, Asiye Karakullukcu, Mailihaba Ailiken, Barıs Otlu, Bilgul Mete, Gokhan Aygun
BACKGROUND: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. METHODS: The prospective study included a total of 4.052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively...
November 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27866944/occurrence-of-carbapenemase-producing-klebsiella-pneumoniae-and-escherichia-coli-in-the-european-survey-of-carbapenemase-producing-enterobacteriaceae-euscape-a-prospective-multinational-study
#12
Hajo Grundmann, Corinna Glasner, Barbara Albiger, David M Aanensen, Chris T Tomlinson, Arjana Tambić Andrasević, Rafael Cantón, Yehuda Carmeli, Alexander W Friedrich, Christian G Giske, Youri Glupczynski, Marek Gniadkowski, David M Livermore, Patrice Nordmann, Laurent Poirel, Gian M Rossolini, Harald Seifert, Alkiviadis Vatopoulos, Timothy Walsh, Neil Woodford, Dominique L Monnet
BACKGROUND: Gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in Europe make it difficult to contain carbapenemase-producing Enterobacteriaceae. We report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in European hospitals. METHODS: National expert laboratories recruited hospitals with diagnostic capacities, who collected the first ten carbapenem non-susceptible clinical isolates of K pneumoniae or E coli and ten susceptible same-species comparator isolates and pertinent patient and hospital information...
November 17, 2016: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/27866753/-molecular-epidemiology-of-carbapenemase-producing-enterobacteriaceae-infection-colonisation-in-a-hospital-in-madrid
#13
Patricia Brañas, Marta Gil, Jennifer Villa, María Ángeles Orellana, Fernando Chaves
INTRODUCTION: A description is presented on the molecular epidemiology of carbapenemase-producing enterobacteriaceae infection in a tertiary hospital. MATERIAL AND METHODS: A study was made on all the carbapenemase-producing enterobacteriaceae isolations obtained between February 2015 and March 2016 in the Hospital Universitario 12 de Octubre (Madrid). Phenotypic and molecular methods were used. RESULTS: A total of 7 bacterial species were identified, with the majority being Klebsiella pneumoniae (K...
November 17, 2016: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/27863949/imipenem-avibactam-a-novel-combination-for-the-rapid-detection-of-carbapenemase-activity-in-enterobacteriaceae-and-acinetobacter-baumannii-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#14
Marina Oviaño, Germán Bou
In the present study, we propose a novel matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for detecting carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii. For this, we analyzed a series of 131 isolates. Among them, a total of 115 Enterobacteriaceae: 79 of them carrying a carbapenemase enzyme (15blaKPC, 7blaNDM, 11blaIMP, 12blaVIM, and 34blaOXA-48) and 16 A. baumannii isolates: 15 of them carrying carbapenemases (10blaOXA-23, 2blaOXA-58, 2blaOXA-24, and 1blaOXA-237)...
February 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27855076/molecular-characterization-of-oxa-48-like-producing-enterobacteriaceae-in-the-czech-republic-evidence-for-horizontal-transfer-of-poxa-48-like-plasmids
#15
Anna Skálová, Kateřina Chudějová, Veronika Rotová, Matej Medvecky, Vendula Študentová, Eva Chudáčková, Pavel Lavicka, Tamara Bergerova, Vladislav Jakubu, Helena Zemlickova, Costas C Papagiannitsis, Jaroslav Hrabák
The aim of this study was to characterize the first cases and outbreaks of OXA-48-like-producing Enterobacteriaceae recovered from hospital settings, in the Czech Republic. During 2013-2015, twenty-two Klebsiella pneumoniae, three Escherichia coli and one Enterobacter cloacae producing OXA-48-like carbapenemases were isolated from 20 patients. Four of the patients were colonized/infected by two or three different OXA-48-like producers. K. pneumoniae isolates were classified into nine STs, with ST101 being the predominant (n=8)...
November 14, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27842873/in-vitro-activities-of-colistin-tigecycline-and-tobramycin-alone-or-in-combination-against-carbapenem-resistant-enterobacteriaceae-strains
#16
Berna Ozbek, Emel Mataracı-Kara, Sevda Er, Melda Ozdamar, Mesut Yilmaz
The aim of this study was to investigate the activities of various antibiotics, alone or in combination, against carbapenem-resistant Enterobacteriaceae (CRE). Minimum inhibitory concentrations (MICs) of meropenem, colistin, tigecycline and tobramycin were determined by microbroth dilution against 40 clinical strains. Carbapenemase-encoding genes were detected by PCR using specific primers. The in vitro synergistic activities of tigecycline, colistin and tobramycin in double antibiotic combinations were determined by the microbroth chequerboard technique, and results were interpreted using the fractional inhibitory concentration index (FICI)...
December 2015: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27802107/clonal-spread-of-colistin-resistant-klebsiella-pneumoniae-coproducing-kpc-and-vim-carbapenemases-in-neonates-at-a-tunisian-university-hospital
#17
Hajer Battikh, Chiraz Harchay, Amal Dekhili, Khaoula Khazar, Fehima Kechrid, Meriem Zribi, Afef Masmoudi, Chedlia Fendri
In this study, we have attempted to report the first clonal spread of colistin-resistant Klebsiella pneumoniae coproducing KPC and VIM carbapenemases in the neonatal unit of Rabta Teaching Hospital of Tunis (Tunisia). This retrospective study was performed from January 1, 2014 to December 31, 2014 in the Microbiology Laboratory at the Rabta University Hospital of Tunis. Twenty-one nonreplicate colistin-resistant K. pneumoniae were isolated from 19 patients hospitalized in the neonatal unit and 2 patients in the adult intensive care unit (ICU)...
November 1, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27795386/evaluation-of-the-carba-np-test-in-oregon-2013
#18
Karim E Morey, Robert Vega, P Maureen Cassidy, Genevieve L Buser, Jaipreet K Rayar, Jeffrey A Myers, Scott J Weissman, Zintars G Beldavs, Christopher D Pfeiffer
Carbapenem-resistant Enterobacteriaceae (CRE) are an urgent public health threat. We evaluated the capacity of the Carba NP test to detect carbapenemase production in 206 isolates: 143 Enterobacteriaceae identified by Oregon's CRE surveillance program in 2013 and 63 known carbapenemase-positive organisms. Overall, test sensitivity and specificity were 89% (59/66 isolates; 95% confidence interval [CI], 81 to 97%) and 100% (140/140 isolates; 95% CI, 98 to 100%), respectively. All KPC, NDM-1, VIM, and IMP producers but no (0/7 isolates) OXA-48-like strains were Carba NP positive prior to a post hoc protocol modification...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27794265/molecular-epidemiology-of-environmental-and-clinical-carbapenemase-producing-gram-negative-bacilli-from-hospitals-in-guelma-algeria-multiple-genetic-lineages-and-first-report-of-oxa-48-in-enterobacter-cloacae
#19
Widad Bouguenoun, Sofiane Bakour, Ahmed Aimen Bentorki, Charbel Al Bayssari, Tarek Merad, Jean-Marc Rolain
This study was designed to investigate environmental colonisation in Algerian hospitals by carbapenem-resistant Gram-negative bacilli (GNB), including molecular characterisation of their resistance, and to perform a comparative molecular analysis between clinical and environmental strains. GNB isolated from hospitalised patients and the hospital environment were identified using microbiological methods and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). Antibiotic susceptibility testing was performed by disk diffusion and Etest methods...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27793962/incfiik-plasmid-harbouring-an-amplification-of-16s-rrna-methyltransferase-encoding-gene-rmth-associated-with-mobile-element-iscr2
#20
Racha Beyrouthy, Frederic Robin, Monzer Hamze, Richard Bonnet
OBJECTIVES: To investigate the resistance mechanisms and genetic support underlying the high resistance level of the Klebsiella pneumoniae strain CMUL78 to aminoglycoside and β-lactam antibiotics. METHODS: Antibiotic susceptibility was assessed by the disc diffusion method and MICs were determined by the microdilution method. Antibiotic resistance genes and their genetic environment were characterized by PCR and Sanger sequencing. Plasmid contents were analysed in the clinical strain and transconjugants obtained by mating-out assays...
October 28, 2016: Journal of Antimicrobial Chemotherapy
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