keyword
MENU ▼
Read by QxMD icon Read
search

OXA-48

keyword
https://www.readbyqxmd.com/read/29348071/a-focused-fragment-library-targeting-the-antibiotic-resistance-enzyme-oxacillinase-48-synthesis-structural-evaluation-and-inhibitor-design
#1
Sundus Akhter, Bjarte Aarmo Lund, Aya Ismael, Manuel Langer, Johan Isaksson, Tony Christopeit, Hanna-Kirsti S Leiros, Annette Bayer
β-Lactam antibiotics are of utmost importance when treating bacterial infections in the medical community. However, currently their utility is threatened by the emergence and spread of β-lactam resistance. The most prevalent resistance mechanism to β-lactam antibiotics is expression of β-lactamase enzymes. One way to overcome resistance caused by β-lactamases, is the development of β-lactamase inhibitors and today several β-lactamase inhibitors e.g. avibactam, are approved in the clinic. Our focus is the oxacillinase-48 (OXA-48), an enzyme reported to spread rapidly across the world and commonly identified in Escherichia coli and Klebsiella pneumoniae...
December 30, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29332105/the-emergence-of-carbapenem-resistant-klebsiella-pneumoniae-isolates-producing-oxa-48-and-ndm-in-the-southern-asir-province-saudi-arabia
#2
Ibrahim A Al-Zahrani, Bander A Alsiri
To identify the prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and the most common types of cabapenemases among CRKP in the Southern (Asir) province hospitals, Saudi Arabia. Methods: The cross-sectional study was conducted between late April and September in 2015. A total of 54 Klebsiella pneumoniae (K. pneumoniae) isolates with reduced sensitivity to carbapenems were obtained from various clinical specimens of the 2 largest hospitals in the Southern province. Minimum inhibitory concentrations (MICs) of carbapenems were confirmed using E-test...
January 2018: Saudi Medical Journal
https://www.readbyqxmd.com/read/29305547/evaluation-of-the-amplidiag-carbar-vre-kit-for-the-accurate-detection-of-carbapenemase-producing-bacteria
#3
Saoussen Oueslati, Delphine Girlich, Laurent Dortet, Thierry Naas
As carbapenemase-producing Gram-negative bacilli (CP-GNB) (Enterobacteriaceae, Pseudomonadaceae and Acinetobacter spp.) are becoming a major public health issue, there is an urgent need for accurate and fast diagnostic tests. Amplidiag® Carba-R+VRE assay is a multiplex nucleic acid-based in-vitro diagnostic test intended for the detection of CP-GNB and vancomycin resistant enterococci (VRE) from cultured colonies.We have evaluated its ability to detect carbapenemases genes in 100 well-characterized GNB, and in 200 consecutive enterobacterial isolates with reduced susceptibility to carbapenems and that were referred to the French National Reference Center for carbapenem-resistance...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29274802/susceptibility-testing-and-detection-of-%C3%AE-lactam-resistance-mechanisms-in-enterobacteriaceae-a-multicentre-national-proficiency-study
#4
María Díez-Aguilar, María-Carmen Conejo, María-Isabel Morosini, Núria Tormo Palop, Concepción Gimeno, Rafael Cantón, Álvaro Pascual
A nationwide study was developed to evaluate the ability of 60 Spanish clinical microbiology laboratories to predict the underlying β-lactam resistance mechanisms of 12 Enterobacteriaceae strains (CCS01-CCS12). Results obtained by two reference laboratories were compared with those reported by the participants that used their own routine susceptibility testing methodology. CLSI interpretive criteria were used in 53.3% of the centres, while 46.7% used EUCAST. The overall categorical agreement (CA) was 85.5%...
December 21, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29237086/enterobacteriaceae-producing-oxa-48-like-carbapenemases-in-poland-2013-january-2017
#5
R Izdebski, A Baraniak, D Zabicka, M Machulska, P Urbanowicz, J Fiett, E Literacka, K Bojarska, A Kozinska, B Zieniuk, W Hryniewicz, M Gniadkowski
Objectives: To analyse OXA-48 (OXA-48/181)-type carbapenemase-producing Enterobacteriaceae reported in Poland from 2013 until January 2017. Methods: Bacterial isolates were typed by PFGE and MLST. Genes coding for OXA-48/181 types and other β-lactamases were amplified and sequenced. Mobile elements with blaOXA-48/181-like genes were PCR mapped. blaOXA-48/181-carrying plasmids were characterized by nuclease S1-hybridization profiling, transfer assays and PCR-based replicon typing, while the chromosomal location of the genes was confirmed by the I-CeuI analysis...
December 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29233256/analysis-of-oxa-204-carbapenemase-producing-enterobacteriaceae-reveals-possible-endoscopy-associated-transmission-france-2012-to-2014
#6
Anaïs Potron, Sandrine Bernabeu, Gaëlle Cuzon, Valérie Pontiès, Hervé Blanchard, Elise Seringe, Thierry Naas, Patrice Nordmann, Laurent Dortet
OXA-48-like beta-lactamase producing bacteria are now endemic in several European and Mediterranean countries. Among this carbapenemase family, the OXA-48 and OXA-181 variants predominate, whereas other variants such as OXA-204 are rarely reported. Here, we report the molecular epidemiology of a collection of OXA-204-positive enterobacterial isolates (n = 29) recovered in France between October 2012 and May 2014. This study describes the first outbreak of OXA-204-producing Enterobacteriaceae in Europe, involving 12 isolates of an ST90 Escherichia coli clone and nine isolates of an ST147 Klebsiella pneumoniae clone...
December 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29228202/activity-of-ceftazidime-avibactam-against-problem-enterobacteriaceae-and-pseudomonas-aeruginosa-in-the-uk-2015-16
#7
David M Livermore, Danièle Meunier, Katie L Hopkins, Michel Doumith, Robert Hill, Rachel Pike, Peter Staves, Neil Woodford
Background: Ceftazidime/avibactam combines an established oxyimino-cephalosporin with the first diazabicyclooctane β-lactamase inhibitor to enter clinical use. We reviewed its activity against Gram-negative isolates, predominantly from the UK, referred for resistance investigation in the first year of routine testing, beginning in July 2015. Methods: Isolates were as received from referring laboratories; there is a bias to submit those with suspected carbapenem resistance...
December 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29205138/virulence-genes-in-isolates-of-klebsiella-pneumoniae-from-the-uk-during-2016-including-among-carbapenemase-gene-positive-hypervirulent-k1-st23-and-non-hypervirulent-types-st147-st15-and-st383
#8
Jane F Turton, Zoë Payne, Amy Coward, Katie L Hopkins, Jack A Turton, Michel Doumith, Neil Woodford
PURPOSE: Klebsiella pneumoniae is a concern because of its multidrug resistance and the ability of hypervirulent types, especially capsular type K1-clonal complex 23 (K1-CC23), to cause community-acquired, life-threatening infections. Hypervirulent types carry an array of virulence genes including rmpA/rmpA2, coding for capsule up-regulation. We sought to identify isolates carrying these elements among submissions to the UK national reference laboratory during 2016. METHODOLOGY: Virulence elements and carbapenemase genes were sought by PCR or from whole genome sequences...
December 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29186953/direct-detection-of-oxa-48-carbapenemase-gene-in-lysate-samples-through-changes-in-mechanical-properties-of-dna-monolayers-upon-hybridization
#9
Carmen M Domínguez, Daniel Ramos, Jesús Mingorance, Jose Luis Garcia Fierro, Javier Tamayo, Montserrat Calleja
Carbapenem-resistant Enterobacteriaceae have become recently an important cause of morbidity and mortality due to healthcare-associated infections. Most commonly used diagnostic methods present time-to-result incompatible with fast and accurate directed therapy. We report here the direct identification of the blaOXA48 gene, which codes for the car-bapenemase OXA-48, in lysate samples from Klebsiella pneumoniae. The method is PCR-free and label-free. It is based on the measurement of changes in the stiffness of DNA self-assembled monolayers anchored to microcantilevers that occur as a consequence of the hybridization...
November 30, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29177610/findings-from-an-outbreak-of-carbapenem-resistant-klebsiella-pneumoniae-emphasize-the-role-of-antibiotic-treatment-for-cross-transmission
#10
Stefan Borgmann, Yvonne Pfeifer, Laura Becker, Beate Rieß, Rabea Siegmund, Ulrich Sagel
PURPOSE: In January 2015, we noticed by rectal swab analyses that seven of 23 patients at an early rehabilitation ward had been colonized with carbapenem-resistant Klebsiella pneumoniae (CKP). Here, we describe risk factors for CKP acquisition. METHODS: In the present study, the outbreak is described and risk factors for CKP acquisition are examined, e.g., antibiotic treatment. Microbiological analyses including corresponding results were examined to study when colonization with CKP occurred and whether patients had suffered from diarrhea...
November 24, 2017: Infection
https://www.readbyqxmd.com/read/29150373/chromagar-msupercarba-and-rapidec%C3%A2-carba-np-test-for-detection-of-carbapenemase-producing-enterobacteriaceae
#11
Meritxell Garcia-Quintanilla, Laurent Poirel, Patrice Nordmann
A novel chromogenic medium CHROMagar mSuperCARBA was evaluated to detect carbapenem-resistant Gram-negatives. This medium is as sensitive and as specific as the SUPERCARBA medium for detecting KPC, MBL and OXA-48-type producers (100% and 100%, respectively) and is compatible with subsequent testing of carbapenemase activity using the RAPIDEC(®) CARBA NP.
October 19, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29146039/clinical-features-and-outcomes-of-bacteraemia-due-to-oxa-48-like-carbapenemase-producing-klebsiella-pneumoniae-in-a-tertiary-hospital
#12
Ana Madueño, Jonathan González-García, María Del Mar Alonso Socas, María Antonia Miguel Gómez, María Lecuona
INTRODUCTION: Limited therapeutic options and high mortality make the management of OXA-48-like carbapenemase-producing Klebsiella pneumoniae (KPOXA-48) bacteraemia complicated. The aim of the study was to describe the clinical characteristics of KPOXA-48 bacteraemia between October 2013 and December 2016. MATERIAL AND METHODS: The variables to analyse were retrospectively collected from medical records. Carbapenemase production was confirmed by phenotypic and molecular methods...
November 13, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29141402/-epidemiology-of-the-carbapenemase-producing-enterobacteriaceae-spread-in-a-community-acute-hospital-and-a-non-acute-rehabilitation-hospital-in-madrid
#13
M López-Dosil, C Bischofberge, D Sáez, C García-Picazo
OBJECTIVE: In Spain, the overall prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is increasing. We describe the epidemiological, clinical and microbiological characteristics features of patients with colonization or infection due to CRE in two hospitals in the north-west of Madrid during two years. One hospital was a community acute hospital and the second one was a non-acute rehabilitation hospital. METHODS: A total of 197 CPE isolates were detected during 2013-2014...
November 14, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29141051/molecular-and-epidemiological-characterization-of-carbapenemase-producing-enterobacteriaceae-in-norway-2007-to-2014
#14
Ørjan Samuelsen, Søren Overballe-Petersen, Jørgen Vildershøj Bjørnholt, Sylvain Brisse, Michel Doumith, Neil Woodford, Katie L Hopkins, Bettina Aasnæs, Bjørg Haldorsen, Arnfinn Sundsfjord
The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological and phenotypic characteristics of all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection of Antimicrobial Resistance. All confirmed CPE isolates were characterized pheno- and genotypically, including by whole genome sequencing (WGS). Patient data were reviewed retrospectively. In total 59 CPE isolates were identified from 53 patients...
2017: PloS One
https://www.readbyqxmd.com/read/29133556/interspecies-transmission-of-the-blaoxa-48-gene-from-klebsiella-pneumoniae-high-risk-clone-st147-to-different-escherichia-coli-clones-in-the-gut-microbiota
#15
Carmen Potel, Adriana Ortega, Lucía Martínez-Lamas, Verónica Bautista, Benito Regueiro, Jesús Oteo
OXA-48-producing K. pneumoniae (OXA-Kpn) have quickly spread in Spain and other European countries (1).….
November 13, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29120560/klebsiella-pneumoniae-oxa-48-in-a-urology-patient-case-report
#16
Ines Jajić, Ana Benčić, Marko Siroglavić, Gernot Zarfel, Boris Ružić, Ivan Pezelj, Branka Bedenić
We present an isolate of Klebsiella pneumoniae OXA-48 isolated in a 68-year-old man who underwent radical prostatectomy due to prostate cancer. The antibiotic susceptibility testing to a wide range of antibiotics was performed by disk diffusion method and determination of minimal inhibitory concentrations. The isolate was classified as multidrug-resistant. It showed intermediate susceptibility to imipenem and meropenem, resistance to ertapenem, and sensitivity only to colistin, amikacin, and trimethoprim-sulfamethoxazole...
March 2017: Acta Clinica Croatica
https://www.readbyqxmd.com/read/29115680/mastdiscs-combi-carba-plus-a-simple-discrimination-method-for-carbapenemase-producing-enterobacteriaceae-including-oxa-48-type-producers
#17
Yusuke Ohsaki, Ryoichi Kubo, Jonathan Hobson, Mya Davies, Shunsuke Osaka, Wataru Hayashi, Yui Taniguchi, Shota Koide, Yukiko Nagano, Noriyuki Nagano
Not only detection of carbapenemases but also identification of their types accurately and rapidly in Enterobacteriaceae is still a major challenge for clinical laboratories in order to prevent the intrusion and the transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MASTDISCS® combi Carba plus disc system in the identification of different carbapenemase types, including OXA-48-type carbapenemase for which no specific enzyme inhibitors have so far been available...
November 8, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/29115369/useful-independent-factors-for-distinguish-infection-and-colonization-in-patients-with-urinary-carbapenemase-producing-enterobacteriaceae-isolation
#18
L López-González, F J Candel, J M Viñuela-Prieto, J González-Del Castillo, A B García, I Pena, I Rodríguez-Avial
OBJECTIVE: The aim of this study is to know epidemiologic and clinical differences among those patients colonized or infected by carbapenemase-producing Enterobacteriaceae (CPE) and develop a predictive model to facilitate the clinical approach concerning to start antimicrobial therapy. METHODS: Observational retrospective cohort study was performed involving all patients with Urine carbapenemase-producing Enterobacteriaceae isolation (UCPEI) between November 2013 and July 2015...
November 7, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29107415/enhanced-detection-of-carbapenemase-producing-enterobacteriaceae-by-an-optimized-phenol-red-assay
#19
Jeremy Surre, Isabelle Canard, Pierrot Bourne-Branchu, Estelle Courbiere, Christine Franceschi, Sonia Chatellier, Alex van Belkum, Mahendrasingh Ramjeet
Screening for the detection of carbapenemase-producing bacteria still encounters issues related to workflow, limit of detection, or qualitative interpretation. We developed a spectrophotometry-based version of the Carba NP phenol red assay (Nordmann et al., 2012) in a microtiter plate format, compatible with low bacterial cell counts. We were able to detect highly active carbapenemases such as KPC and IMP in 30min. A wider range of carbapenemases including OXA-48 were detected using higher inocula, still being competitive compared with currently available phenol red assays...
September 18, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29091199/monitoring-microevolution-of-oxa-48-producing-klebsiella-pneumoniae-st147-in-a-hospital-setting-by-smrt-sequencing
#20
Andreas E Zautner, Boyke Bunk, Yvonne Pfeifer, Cathrin Spröer, Utz Reichard, Helmut Eiffert, Simone Scheithauer, Uwe Groß, Jörg Overmann, Wolfgang Bohne
Objectives: Carbapenemase-producing Klebsiella pneumoniae pose an increasing risk for healthcare facilities worldwide. A continuous monitoring of ST distribution and its association with resistance and virulence genes is required for early detection of successful K. pneumoniae lineages. In this study, we used WGS to characterize MDR blaOXA-48-positive K. pneumoniae isolated from inpatients at the University Medical Center Göttingen, Germany, between March 2013 and August 2014. Methods: Closed genomes for 16 isolates of carbapenemase-producing K...
October 1, 2017: Journal of Antimicrobial Chemotherapy
keyword
keyword
10429
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"