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https://www.readbyqxmd.com/read/28223374/enterobacter-cloacae-complex-isolates-harboring-blanmc-a-or-blaimi-type-class-a-carbapenemase-genes-on-novel-chromosomal-integrative-elements-and-plasmids
#1
David A Boyd, Laura F Mataseje, Ross Davidson, Johannes A Delport, Jeff Fuller, Linda Hoang, Brigitte Lefebvre, Paul Levett, Diane L Roscoe, Barbara M Willey, Michael R Mulvey
Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A blaNMC-A or blaIMI-type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection...
February 21, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28210248/coexistence-of-blaoxa-48-and-truncated-blandm-1-on-different-plasmids-in-a-klebsiella-pneumoniae-isolate-in-china
#2
Lianyan Xie, Yi Dou, Kaixin Zhou, Yue Chen, Lizhong Han, Xiaokui Guo, Jingyong Sun
Objectives: To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical Klebsiella pneumoniae isolate harboring both blaOXA-48 and blaNDM-1 on different plasmids from a Chinese hospital. Methods: The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using Etest and PCR. The plasmids harboring blaOXA-48 and blaNDM-1 were analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis, and hybridization with specific probes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28205502/trends-in-carbapenemase-producing-enterobacteriaceae-france-2012-to-2014
#3
Laurent Dortet, Gaëlle Cuzon, Valérie Ponties, Patrice Nordmann
In 2014, a total of 2,976 Enterobacteriaceae isolates with decreased susceptibility to carbapenems were received at the French Associated National Reference Center for Antibiotic Resistance (NRC) and were characterised for their molecular resistance mechanism to carbapenems and compared with results obtained during 2012 and 2013.The overall number of enterobacterial isolates with decreased susceptibility to carbapenems received at the NRC rapidly increased (more than twofold in two years) with a growing proportion of carbapenemase producers (23...
February 9, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28197140/molecular-characteristics-of-carbapenem-resistant-enterobacter-cloacae-in-ningxia-province-china
#4
Zhiyun Shi, Huizheng Zhao, Gang Li, Wei Jia
The emergence of carbapenem-resistant Enterobacteriaceae (CRE) has become a major public health concern worldwide and a new challenge in the treatment of infectious diseases. The molecular characteristics of Enterobacter cloacae in Ningxia China are unknown. In this study, we reported 10 carbapenem-resistant E. cloacae isolates from the General Hospital of Ningxia Medical University, the largest university hospital in Ningxia between January 2012 and December 2013. Bacteria isolates were identified by Vitek2 compact and the identity of non-duplicate E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28191865/high-carbapenem-resistance-in-clinical-gram-negative-pathogens-isolated-in-egypt
#5
Hazim O Khalifa, Ahmed M Soliman, Ashraf M Ahmed, Toshi Shimamoto, Toshinori Hara, Mitsuyasu Ikeda, Yuta Kuroo, Shizuo Kayama, Motoyuki Sugai, Tadashi Shimamoto
The emergence and spread of carbapenem-resistant gram-negative bacteria poses a serious threat to human health worldwide. Currently, little is known about the molecular mechanisms underlying carbapenem resistance and their prevalence among gram-negative bacteria in Egypt. In this study, we analyzed carbapenemase production in gram-negative bacteria isolated from hospitalized patients in Egypt in 2014. All isolates were subjected to phenotypic and genotypic susceptibility testing for carbapenem resistance. Our results indicated a high level of carbapenem-resistant gram-negative bacteria in Egypt, with 50...
February 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28191309/co-existence-of-blaoxa-23-and-blandm-1-genes-of-acinetobacter-baumannii-isolated-from-nepal-antimicrobial-resistance-and-clinical-significance
#6
Prabhu Raj Joshi, Mahesh Acharya, Trishna Kakshapati, Udomluk Leungtongkam, Rapee Thummeepak, Sutthirat Sitthisak
BACKGROUND: Molecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. METHODS: A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and blaOXA-51 genes...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28167564/multiclonal-expansion-of-klebsiella-pneumoniae-isolates-producing-ndm-1-in-rio-de-janeiro-brazil
#7
Caio Augusto Martins Aires, Polyana Silva Pereira, Carlos Felipe Machado de Araujo, Thiago Pavoni Gomes Chagas, Jane Cleide Oliveira, Sibelle Nogueira Buonora, Rodolpho Mattos Albano, Ana Paula D'Alincourt Carvalho-Assef, Marise Dutra Asensi
We characterized NDM-1-producing Klebsiella spp. isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of blaNDM was determined by S1 PFGE and hybridization. The genotyping were performed by PFGE and MLST. Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin/trometamol, polymyxin B and tigecycline. The spread of NDM-1-producing K. pneumoniae was not associated with a clonal expansion and appears to be associated with Tn3000...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28167547/bacteremia-due-to-carbapenem-resistant-enterobacteriaceae-cre-a-multicenter-clinical-and-molecular-epidemiologic-analysis-in-the-nation-s-epicenter-for-cre
#8
Michael J Satlin, Liang Chen, Gopi Patel, Angela Gomez-Simmonds, Gregory Weston, Angela C Kim, Susan K Seo, Marnie E Rosenthal, Steven J Sperber, Stephen G Jenkins, Camille L Hamula, Anne-Catrin Uhlemann, Michael H Levi, Bettina C Fries, Yi-Wei Tang, Stefan Juretschko, Albert D Rojtman, Tao Hong, Barun Mathema, Michael R Jacobs, Thomas J Walsh, Robert A Bonomo, Barry N Kreiswirth
Although the New York/New Jersey (NY/NJ) area is an epicenter for carbapenem-resistant Enterobacteriaceae (CRE), there are few multicenter studies of CRE from this region. We characterized patients with CRE bacteremia in 2013 at eight NY/NJ medical centers and determined the prevalence of carbapenem resistance among Enterobacteriaceae bloodstream isolates and CRE resistance mechanisms, genetic backgrounds, capsular types (cps), and antimicrobial susceptibilities. Of 121 patients with CRE bacteremia, 50% had cancer or transplantation...
February 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28165472/comprehensive-resistome-analysis-reveals-the-prevalence-of-ndm-and-mcr-1-in-chinese-poultry-production
#9
Yang Wang, Rongmin Zhang, Jiyun Li, Zuowei Wu, Wenjuan Yin, Stefan Schwarz, Jonathan M Tyrrell, Yongjun Zheng, Shaolin Wang, Zhangqi Shen, Zhihai Liu, Jianye Liu, Lei Lei, Mei Li, Qidi Zhang, Congming Wu, Qijing Zhang, Yongning Wu, Timothy R Walsh, Jianzhong Shen
By 2030, the global population will be 8.5 billion, placing pressure on international poultry production, of which China is a key producer(1). From April 2017, China will implement the withdrawal of colistin as a growth promoter, removing over 8,000 tonnes per year from the Chinese farming sector(2). To understand the impact of banning colistin and the epidemiology of multi-drug-resistant (MDR) Escherichia coli (using blaNDM and mcr-1 as marker genes), we sampled poultry, dogs, sewage, wild birds and flies...
February 6, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28156193/characterization-of-ndm-7-carbapenemase-producing-escherichia-coli-isolates-in-the-arabian-peninsula
#10
Tibor Pál, Akela Ghazawi, Dania Darwish, Laura Villa, Alessandra Carattoli, Rayhan Hashmey, Zulfa Aldeesi, Wafaa Jamal, Vincent Rotimi, Amina Al-Jardani, Seif S Al-Abri, Ágnes Sonnevend
AIM: The purpose of this study was to characterize the New Delhi metallo-beta lactamase (NDM)-7-producing Enterobacteriaceae isolated in the Arabian Peninsula. METHODS: Enterobacteriaceae identified to carry blaNDM-7 in a collection of 157 NDM-producing isolates from Kuwait, Oman, Saudi Arabia, and the United Arab Emirates (UAE) were investigated for their antibiotic and disinfectant susceptibility, and resistance gene content. The virulence profile, phylogenetic and sequence types of the isolates were also determined...
February 3, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28151407/rapid-detection-of-carbapenemases-in-enterobacteriaceae-evaluation-of-the-resist-3-o-k-n-oxa-48-kpc-ndm-multiplexed-lateral-flow-assay
#11
David W Wareham, Muhd Haziq F Abdul Momin
The identification, treatment and control of carbapenem resistant Enterobacteriaceae (CRE) infections are a major challenge for healthcare institutions and diagnostic laboratories worldwide.….
February 1, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28150578/resistotyping-phenotyping-and-genotyping-of-new-delhi-metallo-%C3%AE-lactamase-ndm-among-gram-negative-bacilli-from-iranian-patients
#12
Dariush Shokri, Mohammad Rabbani Khorasgani, Seyed Masih Fatemi, Abbas Soleimani-Delfan
PURPOSE: The purpose of this study was investigation of New Delhi metallo-β-lactamase (NDM) production among gram negative bacilli. METHODOLOGY: Antibiogram-Resistotyping and detection of NDM in clinical isolates of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa and comparative evaluation of the diagnostic performance of three phenotypic methods for NDM detection considering PCR as gold standard were performed. Minimum Inhibitory Concentration (MIC) of antibiotics against NDM positive strains by E-test and clonal relationship analysis using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) in these strains were determined...
February 1, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28139430/prevalence-of-mcr-1-in-escherichia-coli-and-klebsiella-pneumoniae-recovered-from-bloodstream-infections-in-china-a-multicentre-longitudinal-study
#13
Jingjing Quan, Xi Li, Yan Chen, Yan Jiang, Zhihui Zhou, Huichuan Zhang, Lu Sun, Zhi Ruan, Ye Feng, Murat Akova, Yunsong Yu
BACKGROUND: Polymyxin antibiotics are used as last-resort therapies to treat infections caused by multidrug-resistant Gram-negative bacteria. The plasmid-mediated colistin resistance determinant MCR-1 has been identified in Enterobacteriaceae in China. We did this study to investigate the prevalence of the mcr-1 gene in clinical isolates from patients with bloodstream infections in China. METHODS: Clinical isolates of Escherichia coli and Klebsiella pneumoniae were collected from patients with bloodstream infections at 28 hospitals in China, then screened for colistin resistance by broth microdilution and for the presence of the mcr-1 gene by PCR amplification...
January 27, 2017: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/28131738/occurrence-of-blandm-7-within-incx3-type-plasmid-of-escherichia-coli-from-india
#14
Deepjyoti Paul, Amitabha Bhattacharjee, Birson Ingti, Nargis Alom Choudhury, Anand Prakash Maurya, Debadatta Dhar, Atanu Chakravarty
BACKGROUND: New-Delhi metallo-β-lactamase-7 with higher hydrolytic activity than its ancestor NDM-1 is emerging across the globe including India. In this study, we have investigated the genetic context of blaNDM-7 and alteration in plasmid copy number under concentration gradient carbapenem stress. MATERIALS AND METHODS: Six blaNDM-7 producing Escherichia coli isolates were obtained from Silchar Medical College and Hospital and the co-existence of other β-lactamases and transferability of this resistant determinant was determined by transformation and conjugation assay followed by typing of the plasmid by PBRT method...
January 25, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28111849/sulfonylurea-challenge-test-in-subjects-diagnosed-with-type-1-diabetes-mellitus
#15
Maria S Remedi, Mareen Thomas, Colin G Nichols, Bess A Marshall
BACKGROUND: Patients with early onset diabetes because of defects in glucose-stimulated insulin secretion (GSIS) may respond better to sulfonylureas than insulin treatment. Such patients include those with monogenic disorders, who can be differentiated from autoimmune type 1 diabetes mellitus (T1DM) by genetic testing. Genetic testing is expensive and unknown defects in GSIS would not be diagnosed. AIMS: We propose a sulfonylurea challenge test to identify patients who have been clinically diagnosed with T1DM, but those who maintain a preferentially sulfonylurea-responsive insulin secretion...
January 23, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28109845/outbreak-of-nosocomial-ndm-1-producing-klebsiella-pneumoniae-st1419-in-a-neonatal-unit
#16
Jing Yu, Yue Wang, Zhongju Chen, Xuhui Zhu, Lei Tian, Li Li, Ziyong Sun
OBJECTIVES: The aim of this study was to characterise carbapenem-resistant Klebsiella pneumoniae isolates recovered from neonatal clinical specimens over a 4-month period. METHODS: Seven carbapenem-resistant K. pneumoniae isolates were analysed. Antibiotic susceptibilities of the isolates were determined by the agar dilution method, and the drug resistance genes were evaluated by PCR. Clonal relatedness of the isolates was assessed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST)...
January 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28109787/synthesis-and-in-vitro-activity-of-dicationic-indolyl-diphenyl-ethers-as-novel-potent-antibiotic-agents-against-drug-resistant-bacteria
#17
Xiaofang Chen, Xinxin Hu, Yanbin Wu, Yonghua Liu, Cong Bian, Tongying Nie, Xuefu You, Laixing Hu
A series of 4,4'-bis-[2-(6-N-substituted-amidino)indolyl] diphenyl ether have been synthesized and tested for their in vitro antibacterial activity including a range of Gram-positive and Gram-negative pathogens and cytotoxicity. Most of these compounds have mainly shown anti-Gram positive bacteria activities especially against drug resistant bacterial strains MRSA, MRSE and VRE. The anti-MRSA and anti-MRSE activities of compound 7a and 7j were more potent than that of the lead compound 2, levofloxacin and vancomycin...
January 10, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28104655/first-complete-providencia-rettgeri-genome-sequence-the-ndm-1-producing-clinical-strain-rb151
#18
R Alejandro Marquez-Ortiz, Leanne Haggerty, Eby M Sim, Carolina Duarte, Betsy E Castro-Cardozo, Mauricio Beltran, Sandra Saavedra, Natasha Vanegas, Javier Escobar-Perez, Nicola K Petty
Providencia rettgeri is an opportunistic bacterial pathogen of clinical significance due to its association with urinary tract infections and multidrug resistance. Here, we report the first complete genome sequence of P. rettgeri The genome of strain RB151 consists of a 4.8-Mbp chromosome and a 108-kbp blaNDM-1-positive plasmid.
January 19, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28104504/diversity-of-new-delhi-metallo-beta-lactamase-producing-bacteria-in-china
#19
Xiaofeng Hu, Xuebing Xu, Xu Wang, Wencheng Xue, Haijian Zhou, Ling Zhang, Qiuxia Ma, Rongtao Zhao, Guozheng Li, Peng Li, Chuanfu Zhang, Yun Shi, Jian Wang, Leili Jia, Rongzhang Hao, Ligui Wang, Dayang Zou, Xuelin Liu, Shaofu Qiu, Hongbin Song, Yansong Sun
OBJECTIVES: The prevalence and dissemination of diverse NDM-producing bacteria in China was investigated. METHODS: We collected 1,162 isolates from 8 cities during December 2013∼May 2015 in China. The NDM-positive strains as well as the NDM genotypes in these sample were detected via Vitek 2 compact system (bioMérieux, France), 16S rRNA gene sequencing, PCR and an S1- pulsed-field gel electrophoresis assay and Southern blot hybridization. The horizontal-transfer capability of the blaNDM gene was assessed by filter mating by using a standard E...
January 16, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28102514/carbapenemase-producing-klebsiella-pneumoniae-bloodstream-infection-in-critically-ill-patients-risk-factors-and-predictors-of-mortality
#20
M Papadimitriou-Olivgeris, F Fligou, C Bartzavali, A Zotou, A Spyropoulou, K Koutsileou, S Vamvakopoulou, N Sioulas, V Karamouzos, E D Anastassiou, I Spiliopoulou, M Christofidou, M Marangos
A significant increase in carbapenemase-producing Klebsiella pneumoniae (CP-Kp) bacteraemias has been observed worldwide. The objective of the present work was to study the risk factors and predictors of mortality of CP-Kp bacteraemias among critically ill patients. During a 4-year period (2012-3015), a matched 1:2 case-control study was conducted. Klebsiella pneumoniae was identified by Vitek 2 technology. Antibiotic susceptibility was performed by the agar disc diffusion method and Etest. The presence of the bla KPC, bla VIM and bla NDM genes was confirmed by polymerase chain reaction (PCR)...
January 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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