keyword
MENU ▼
Read by QxMD icon Read
search

Culture change

keyword
https://www.readbyqxmd.com/read/28938734/hyperspectral-microscopy-can-detect-metabolic-heterogeneity-within-bovine-post-compaction-embryos-incubated-under-two-oxygen-concentrations-7-versus-20
#1
Melanie L Sutton-McDowall, Martin Gosnell, Ayad G Anwer, Melissa White, Malcolm Purdey, Andrew D Abell, Ewa M Goldys, Jeremy G Thompson
STUDY QUESTION: Can we separate embryos cultured under either 7% or 20% oxygen atmospheres by measuring their metabolic heterogeneity? SUMMARY ANSWER: Metabolic heterogeneity and changes in metabolic profiles in morula exposed to two different oxygen concentrations were not detectable using traditional fluorophore and two-channel autofluorescence but were detectable using hyperspectral microscopy. WHAT IS KNOWN ALREADY: Increased genetic and morphological blastomere heterogeneity is associated with compromised developmental competence of embryos and currently forms the basis for embryo scoring within the clinic...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28938611/dickkopf-1-induces-angiogenesis-via-vegf-receptor-2-regulation-independent-of-the-wnt-signaling-pathway
#2
Sung Hoon Choi, Hyemi Kim, Hyun Gyu Lee, Beom Kyung Kim, Jun Yong Park, Do Young Kim, Sang Hoon Ahn, Kwang-Hyub Han, Seung Up Kim
Tumor angiogenesis is essential for invasive tumor growth and metastasis. Dickkopf-1 (DKK-1), an antagonist of Wnt signaling, participates in tumor development and progression. We evaluated whether DKK-1 stimulation induces angiogenesis and the endothelial-mesenchymal transition (EnMT). Human umbilical vein endothelial cells (HUVECs) were stimulated with recombinant DKK-1 (rDDK-1) or conditioned medium from a culture of DKK-1-transfected 293 cells. Following stimulation, the expression levels of angiogenesis-related factors and EnMT related markers were determined by immunoblot assays...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938596/vitamin-d3-induces-vitamin-d-receptor-and-hdac11-binding-to-relieve-the-promoter-of-the-tight-junction-proteins
#3
Feng-Hua Liu, Shan-Shan Li, Xiao-Xi Li, Shuai Wang, Mao-Gang Li, Li Guan, Tian-Gang Luan, Zhi-Gang Liu, Zhan-Ju Liu, Ping-Chang Yang
Intestinal epithelial barrier dysfunction and vitamin D (VitD)-deficiency play a critical role in a large number of diseases. The histone deacetylases (HDAC) are associated with a large number of immune diseases. This study tests a hypothesis that the interaction between VitD and HDAC is associated with the regulation of epithelial barrier functions. In this study, human intestinal epithelial cell line, T84 cells, was cultured into monolayers to be used as a model to test the epithelial barrier functions. We observed that in a VitD-deficient environment, the T84 monolayer barrier function was compromised...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938460/huwe1-regulates-the-establishment-and-maintenance-of-spermatogonia-by-suppressing-dna-damage-response
#4
Kin Lam Fok, Rohini Bose, Kai Sheng, Ching-Wen Chang, Mira Katz-Egorov, Martine Culty, Sicheng Su, Ming Yang, Ye Chun Ruan, Hsiao-Chang Chan, Antonio Iavarone, Anna Lasorella, Regina Cencic, Jerry Pelletier, Makoto Nagano, Wenming Xu, Simon S Wing
Spermatogenesis is sustained by a heterogeneous population of spermatogonia which includes the spermatogonial stem cells. However, the mechanisms underlying their establishment from gonocyte embryonic precursors and their maintenance thereafter remain largely unknown. Here, we report that inactivation of the ubiquitin ligase Huwe1 in male germ cells in mice led to the degeneration of spermatogonia in neonates and resulted in a Sertoli cell-only phenotype in the adult. Huwe1 KO gonocytes showed a decrease in mitotic re-entry which inhibited their transition to spermatogonia...
August 25, 2017: Endocrinology
https://www.readbyqxmd.com/read/28937988/differential-effects-of-rice-bran-cultivars-to-limit-salmonella-typhimurium-in-chicken-cecal-in-vitro-incubations-and-impact-on-the-cecal-microbiome-and-metabolome
#5
Peter M Rubinelli, Sun Ae Kim, Si Hong Park, Stephanie M Roto, Nora Jean Nealon, Elizabeth P Ryan, Steven C Ricke
In this study, rice brans from different cultivars (Calrose, Jasmine, and Red Wells) were assessed for their ability to inhibit Salmonella enterica serovar Typhimurium using an in vitro mixed anaerobic culture system containing cecal microbiota obtained from broilers of different ages. Salmonella Typhimurium was added to controls (feed only, cecal only, and feed + cecal material) and treatments (feed + cecal + different rice brans) and S. Typhimurium populations were enumerated at 0, 24, and 48 h. Two experimental conditions were applied 1) unadapted condition in which S...
2017: PloS One
https://www.readbyqxmd.com/read/28937579/pyrethroids-inhibit-k2p-channels-and-activate-sensory-neurons-basis-of-insecticide-induced-paraesthesias
#6
Aida Castellanos, Alba Andres, Laura Bernal, Gerard Callejo, Nuria Comes, Arcadi Gual, Jonathan P Giblin, Carolina Roza, Xavier Gasull
Pyrethroid insecticides are widely used for pest control, in agriculture or in human public health commonly as a topical treatment for scabies and head lice. Exposure to pyrethroids such as permethrin or tetramethrin (TM) causes sensory alterations such as transient pain, burning, stinging sensations and paraesthesias. Despite the well-known effects of pyrethroids on sodium channels, actions on other channels that control sensory neuron excitability are less studied. Given the role of two-pore domain potassium (K2P) channels in modulating sensory neuron excitability and firing, both in physiological and pathological conditions, we examined the effect of pyrethroids on K2P channels mainly expressed in sensory neurons...
September 19, 2017: Pain
https://www.readbyqxmd.com/read/28937032/development-and-standardization-of-a-new-cognitive-assessment-test-battery-for-chinese-aphasic-patients-a-preliminary-study
#7
Ji-Bao Wu, Zhi-Hong Lyu, Xiao-Jia Liu, Hai-Peng Li, Qi Wang
BACKGROUND: Nonlinguistic cognitive impairment has become an important issue for aphasic patients, but currently there are few neuropsychological cognitive assessment tests for it. To get more information on cognitive impairment of aphasic patients, this study aimed to develop a new cognitive assessment test battery for aphasic patients, the Non-language-based Cognitive Assessment (NLCA), and evaluate its utility in Chinese-speaking patients with aphasia. METHODS: The NLCA consists of five nonverbal tests, which could assess five nonlinguistic cognitive domains such as visuospatial functions, attention test, memory, reasoning, and executive functions of aphasic patients...
October 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28936961/-effects-of-small-rna-interference-targeting-mammalian-target-of-rapamycin-on-paraquat-induced-pulmonary-fibrosis-in-rats
#8
Wenbin Yang, Xiaoqing Zhao, Ran Liang, Da Chen
OBJECTIVE: To investigate the effects of small RNA interference targeting mammalian target of rapamycin (mTOR) expression on paraquat-induced pulmonary fibrosis in rats. METHODS: Human embryonic kidney cells HEK-293 were cultured in vitro. The mTOR small interfering RNA (mTOR-siRNA) expression plasmid transfection lentivirus was constructed, and non-specific sequence plasmid with no homology to mTOR gene was set as the control. Seventy-two healthy male Sprague-Dawley (SD) rats were randomly divided into normal saline (NS) control group, paraquat model group, mTOR unrelated sequence group, and mTOR-siRNA group, with 18 rats in each group...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936960/-role-of-phosphorylated-moesin-in-the-injury-of-pulmonary-microvascular-endothelial-cells-of-rats-and-its-mechanism
#9
Gang Wang, Gengyun Sun
OBJECTIVE: To investigate the role of phosphorylated Moesin (p-Moesin) in the injury of pulmonary microvascular endothelial cells (PMVECs) of rats induced by tumor necrosis factor-α (TNF-α), and to approach the impact of Rac1 signal pathway on Moesin phosphorylation. METHODS: PMVECs of rats were cultured in vitro and passed on to the third generation, and the TNF-α time-effect experiment, dose-effect experiment and Rac1 signaling pathway intervention experiment were performed respectively...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936955/-analysis-of-characteristics-of-bacteria-in-respiratory-tract-infection-in-2013-2016-in-heibei-3a-hospital-a-single-center-report-of-7-497-patients
#10
Lili Hou, Lili Liu, Ping Dang, Guannan Kang, Qinfeng Zhang, Dongling Li
OBJECTIVE: To analyze the changes and characteristics of respiratory tract bacteria in Hebei 3A Hospital, and to provide new rationale for clinical diagnosis and treatment. METHODS: A single-center retrospective analysis was conducted. 7 497 patients with respiratory tract infection admitted to Hebei Chest Hospital from January 2013 to December 2016 were enrolled. Deep sputum was collected, and the bacterial cultures and susceptibility analysis was conducted in sputum and upper respiratory secretions were collected by fiberoptic bronchoscopy...
September 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28936789/toxic-synergism-between-quinolinic-acid-and-glutaric-acid-in-neuronal-cells-is-mediated-by-oxidative-stress-insights-to-a-new-toxic-model
#11
Paula Pierozan, Ana Laura Colín-González, Helena Biasibetti, Janaina Camacho da Silva, Angela Wyse, Moacir Wajner, Abel Santamaria
It has been shown that synergistic toxic effects of quinolinic acid (QUIN) and glutaric acid (GA), both in isolated nerve endings and in vivo conditions, suggest the contribution of these metabolites to neurodegeneration. However, this synergism still requires a detailed characterization of the mechanisms involved in cell damage during its occurrence. In this study, the effects of subtoxic concentrations of QUIN and/or GA were tested in neuronal cultures, co-cultures (neuronal cells + astrocytes), and mixed cultures (neuronal cells + astrocytes + microglia) from rat cortex and striatum...
September 21, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28936777/phytosphingosine-enhances-moisture-level-in-human-skin-barrier-through-stimulation-of-the-filaggrin-biosynthesis-and-degradation-leading-to-nmf-formation
#12
Hyun Kyung Choi, Young Hoon Cho, Eun Ok Lee, Jin Wook Kim, Chang Seo Park
Phytosphingosine (PHS) is a sphingoid that is a key component of phytoceramides NP, AP and EOP. PHS has been known to have anti-inflammation and antimicrobial activities and to stimulate epidermal differentiation. In addition, it is reported that PHS treatment notably increased phytoceramide content in keratinocytes. In this study, we tried to investigate whether PHS has any effect on the maturation of corneocytes such as formation of cornified envelope and natural moisturizing factor (NMF) that is also an essential event during the formation of skin barrier, stratum corneum...
September 21, 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28936234/the-effects-of-co2-and-h2-on-co-metabolism-by-pure-and-mixed-microbial-cultures
#13
Sofia Esquivel-Elizondo, Anca G Delgado, Bruce E Rittmann, Rosa Krajmalnik-Brown
BACKGROUND: Syngas fermentation, the bioconversion of CO, CO2, and H2 to biofuels and chemicals, has undergone considerable optimization for industrial applications. Even more, full-scale plants for ethanol production from syngas fermentation by pure cultures are being built worldwide. The composition of syngas depends on the feedstock gasified and the gasification conditions. However, it remains unclear how different syngas mixtures affect the metabolism of carboxidotrophs, including the ethanol/acetate ratios...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28936174/the-effect-of-velvet-antler-proteins-on-cardiac-microvascular-endothelial-cells-challenged-with-ischemia-hypoxia
#14
Xiang Xiao, Shuqiang Xu, Lin Li, Min Mao, Jinping Wang, Yanjun Li, Ziwei Wang, Fei Ye, Li Huang
Velvet antler (VA) is a precious traditional Chinese medicine that is capable of repeated regeneration. Based on the Chinese medicine theory of coordination the heart and kidneys, VA has been employed to treat heart diseases, including ischemic heart disease, heart failure, and arrhythmia. We examined the effects of VA proteins on primary cardiac microvascular endothelial cells (CMECs) that were subjected to ischemia-hypoxia (IH) to investigate their effects on and mechanism of action in the treatment of ischemic heart disease...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28936133/migrants-decision-process-shaping-work-destination-choice-the-case-of-long-term-care-work-in-the-united-kingdom-and-norway
#15
Karen Christensen, Shereen Hussein, Mohamed Ismail
Escalating demands for formal long-term care (LTC) result in the reliance on migrant workers in many developed countries. Within Europe, this is currently framed by progressive European immigration policies favouring inter-European mobility. Using the UK and Norway as case studies, this article has two main aims: (1) to document changes in the contribution of European Union (EU) migrants to the LTC sectors in Western Europe, and (2) to gain further understanding of migrants' decision-processes relating to destination and work choices...
September 2017: European Journal of Ageing
https://www.readbyqxmd.com/read/28935946/population-resequencing-of-european-mitochondrial-genomes-highlights-sex-bias-in-bronze-age-demographic-expansions
#16
Chiara Batini, Pille Hallast, Åshild J Vågene, Daniel Zadik, Heidi A Eriksen, Horolma Pamjav, Antti Sajantila, Jon H Wetton, Mark A Jobling
Interpretations of genetic data concerning the prehistory of Europe have long been a subject of great debate, but increasing amounts of ancient and modern DNA data are now providing new and more informative evidence. Y-chromosome resequencing studies in Europe have highlighted the prevalence of recent expansions of male lineages, and focused interest on the Bronze Age as a period of cultural and demographic change. These findings contrast with phylogeographic studies based on mitochondrial DNA (mtDNA), which have been interpreted as supporting expansions from glacial refugia...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935906/resilience-of-the-larval-slipper-limpet-crepidula-onyx-to-direct-and-indirect-diet-effects-of-ocean-acidification
#17
Elizaldy A Maboloc, Kit Yu Karen Chan
Ocean acidification (OA) is known to directly impact larval physiology and development of many marine organisms. OA also affects the nutritional quality and palatability of algae, which are principal food sources for many types of planktonic larvae. This potential indirect effect of OA via trophic interactions, however, has not been fully explored. In this study, veligers of Crepidula onyx were exposed to different pH levels representing the ambient (as control) and low pH values (pH 7.7 and pH 7.3) for 14 days, and were fed with Isochrysis galbana cultured at these three respective pHs...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935865/melanoblast-development-coincides-with-the-late-emerging-cells-from-the-dorsal-neural-tube-in-turtle-trachemys-scripta
#18
Ritva Rice, Judith Cebra-Thomas, Maarja Haugas, Juha Partanen, David P C Rice, Scott F Gilbert
Ectothermal reptiles have internal pigmentation, which is not seen in endothermal birds and mammals. Here we show that the development of the dorsal neural tube-derived melanoblasts in turtle Trachemys scripta is regulated by similar mechanisms as in other amniotes, but significantly later in development, during the second phase of turtle trunk neural crest emigration. The development of melanoblasts coincided with a morphological change in the dorsal neural tube between stages mature G15 and G16. The melanoblasts delaminated and gathered in the carapacial staging area above the neural tube at G16, and differentiated into pigment-forming melanocytes during in vitro culture...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28935818/increased-mir-124-3p-in-microglial-exosomes-following-traumatic-brain-injury-inhibits-neuronal-inflammation-and-contributes-to-neurite-outgrowth-via-their-transfer-into-neurons
#19
Shan Huang, Xintong Ge, Jinwen Yu, Zhaoli Han, Zhenyu Yin, Ying Li, Fanglian Chen, Haichen Wang, Jianning Zhang, Ping Lei
Neuronal inflammation is the characteristic pathologic change of acute neurologic impairment and chronic traumatic encephalopathy after traumatic brain injury (TBI). Inhibiting the excessive inflammatory response is essential for improving the neurologic outcome. To clarify the regulatory mechanism of microglial exosomes on neuronal inflammation in TBI, we focused on studying the impact of microglial exosomal miRNAs on injured neurons in this research. We used a repetitive (r)TBI mouse model and harvested the injured brain extracts from the acute to the chronic phase of TBI to treat cultured BV2 microglia in vitro The microglial exosomes were collected for miRNA microarray analysis, which showed that the expression level of miR-124-3p increased most apparently in the miRNAs...
September 21, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28935667/upregulation-of-herv-k-is-linked-to-immunity-and-inflammation-in-pulmonary-arterial-hypertension
#20
Toshie Saito, Kazuya Miyagawa, Shih-Yu Chen, Rasa Tamosiuniene, Lingli Wang, Orr Sharp, Erik Samayoa, Daisuke Harada, Jan-Renier A J Moonen, Aiqin Cao, Pin-I Chen, Jan K Hennigs, Mingxia Gu, Caiyun G Li, Ryan D Leib, Dan Li, Christopher M Adams, Patricia A Del Rosario, Matthew A Bill, Francois Haddad, Jose G Montoya, William Robinson, Wendy J Fantl, Garry P Nolan, Roham T Zamanian, Mark R Nicolls, Charles Y Chiu, Maria E Ariza, Marlene Rabinovitch
Background -Immune dysregulation has been linked to occlusive vascular remodeling in pulmonary arterial hypertension (PAH) that is hereditary, idiopathic or associated with other conditions. Circulating autoantibodies, lung perivascular lymphoid tissue and elevated cytokines have been related to PAH pathogenesis but without clear understanding of how these abnormalities are initiated, perpetuated and connected in the progression of disease. We therefore set out to identify specific target antigens in PAH lung immune complexes as a starting point toward resolving these issues to better inform future application of immunomodulatory therapies...
September 21, 2017: Circulation
keyword
keyword
104277
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"