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https://www.readbyqxmd.com/read/29352710/changes-in-hepg2-spheroid-behavior-induced-by-differences-in-the-gap-distance-between-spheroids-in-a-micropatterned-culture-system
#1
Daisuke Miyamoto, Takuya Hara, Ai Hyakutake, Kohji Nakazawa
Micropatterning is a promising technique for modulating culture environments. In this study, we investigated the effect of spheroid separation distance on their properties in a micropatterned chip of HepG2 spheroids. The basic chip design consisted of 37 collagen spots (300 μm in diameter) in a hexagonal arrangement on a glass substrate; the region without collagen-spots was modified by polyethylene glycol to create the non-adhesive surface. Three similar chips were fabricated with gap distances between collagen-spots of 500, 1000, and 1500 μm...
January 15, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29352651/catheter-injected-bone-marrow-mesenchymal-stem-cells-induce-efficacious-occlusion-of-arteriovenous-nidus-in-a-swine-model
#2
Joseph Touma, Jianping Dai, Andre Gaston, Marianne Gervais, Eric Allaire
OBJECTIVES: Arteriovenous malformations (AVMs) are complex vascular lesions. Surgical excision is the treatment of choice, but is often not achievable. Embolo-sclerotherapy alone is associated with high recurrence rates. This study tested the hypothesis that seeding hydrogel conditioned bone marrow derived mesenchymatous stem cells (BM-MSCs) in an AVM nidus model induces solid microvascular occlusion through endoluminal tissue growth. METHODS: AVMs were modelled as arteriovenous microvascular nidus, using swine rete mirabile, a plexiform intracranial structure composed of arterial microvessels that extensively anastomose...
January 15, 2018: European Journal of Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29352540/insight-in-schizophrenia-spectrum-disorders-relationship-with-behavior-mood-and-perceived-quality-of-life-underlying-causes-and-emerging-treatments
#3
Paul H Lysaker, Michelle L Pattison, Bethany L Leonhardt, Scott Phelps, Jenifer L Vohs
Poor insight in schizophrenia is prevalent across cultures and phases of illness. In this review, we examine the recent research on the relationship of insight with behavior, mood and perceived quality of life, on its complex roots, and on the effects of existing and emerging treatments. This research indicates that poor insight predicts poorer treatment adherence and therapeutic alliance, higher symptom severity and more impaired community function, while good insight predicts a higher frequency of depression and demoralization, especially when coupled with stigma and social disadvantage...
February 2018: World Psychiatry: Official Journal of the World Psychiatric Association (WPA)
https://www.readbyqxmd.com/read/29352391/functional-recovery-by-application-of-human-dedifferentiated-fat-cells-on-cerebral-infarction-mice-model
#4
Tomoki Kakudo, Naotaka Kishimoto, Tomohiro Matsuyama, Yoshihiro Momota
Elderly people whose daily activities have declined due to a cerebrovascular disorder may suffer from dysphagia and may find oral hygiene difficult. Therefore, it is important to establish an effective therapy for the underlying cerebrovascular disorder. Dedifferentiated fat cells (DFAT) were obtained from mature adipocytes isolated from human buccal adipose pads in a ceiling culture. DFAT expressed the neural markers Nestin and SOX2. Flow cytometric analysis revealed that the cells had properties similar to mesenchymal stem cells...
January 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29351879/the-importance-of-monochromatic-lights-in-the-production-of-phenolic-acids-and-flavonoids-in-shoot-cultures-of-aronia-melanocarpa-aronia-arbutifolia-and-aronia%C3%A2-%C3%A3-%C3%A2-prunifolia
#5
Agnieszka Szopa, Anna Starzec, Halina Ekiert
Shoot cultures of Aronia melanocarpa, A. arbutifolia and A. × prunifolia were maintained on Murashige and Skoog medium with 1 mg/l each of BA and NAA under monochromatic lights (far-red, red, blue lights, UV-A-irradiation), in darkness, and under white light (control). HPLC-DAD analyses of 19 phenolic acids and 11 flavonoids in methanolic extracts from the shoots revealed in all of them the presence of three depsides (chlorogenic, neochlorogenic and rosmarinic acids), protocatechuic acid, four flavonoid glycosides (cynaroside, quercitrin, hyperoside and rutoside), and additionally, in A...
January 10, 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29351865/interventions-to-support-effective-communication-between-maternity-care-staff-and-women-in-labour-a-mixed-methods-systematic-review
#6
Yan-Shing Chang, Kirstie Coxon, Anayda Gerarda Portela, Marie Furuta, Debra Bick
OBJECTIVES: the objectives of this review were (1) to assess whether interventions to support effective communication between maternity care staff and healthy women in labour with a term pregnancy could improve birth outcomes and experiences of care; and (2) to synthesize information related to the feasibility of implementation and resources required. DESIGN: a mixed-methods systematic review. SETTING AND PARTICIPANTS: studies which reported on interventions aimed at improving communication between maternity care staff and healthy women during normal labour and birth, with no apparent medical or obstetric complications, and their family members were included...
December 27, 2017: Midwifery
https://www.readbyqxmd.com/read/29351847/phosphorylation-of-ezh2-by-ampk-suppresses-prc2-methyltransferase-activity-and-oncogenic-function
#7
Lixin Wan, Kexin Xu, Yongkun Wei, Jinfang Zhang, Tao Han, Christopher Fry, Zhao Zhang, Yao Vickie Wang, Liyu Huang, Min Yuan, Weiya Xia, Wei-Chao Chang, Wen-Chien Huang, Chien-Liang Liu, Yuan-Ching Chang, Jinsong Liu, Yun Wu, Victor X Jin, Xiangpeng Dai, Jianfeng Guo, Jia Liu, Shulong Jiang, Jin Li, John M Asara, Myles Brown, Mien-Chie Hung, Wenyi Wei
Sustained energy starvation leads to activation of AMP-activated protein kinase (AMPK), which coordinates energy status with numerous cellular processes including metabolism, protein synthesis, and autophagy. Here, we report that AMPK phosphorylates the histone methyltransferase EZH2 at T311 to disrupt the interaction between EZH2 and SUZ12, another core component of the polycomb repressive complex 2 (PRC2), leading to attenuated PRC2-dependent methylation of histone H3 at Lys27. As such, PRC2 target genes, many of which are known tumor suppressors, were upregulated upon T311-EZH2 phosphorylation, which suppressed tumor cell growth both in cell culture and mouse xenografts...
January 18, 2018: Molecular Cell
https://www.readbyqxmd.com/read/29351778/a-qualitative-analysis-of-men-s-involvement-in-maternal-and-child-health-as-a-policy-intervention-in-rural-central-malawi
#8
Elizabeth Mkandawire, Sheryl L Hendriks
BACKGROUND: Men's involvement in maternal and child health presents an opportunity for the advancement of maternal and child nutrition as men often play a key role in decision-making particularly regarding women's reproductive health. While most research on men's involvement in maternal and child health has focused on men's participation in antenatal care, this study focuses specifically on men's involvement in maternal and child nutrition. The purpose of the study is to explore how men's involvement is conceptualised in rural Central Malawi, highlighting the key factors influencing men's involvement in maternal and child health...
January 19, 2018: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29351650/are-evidence-based-community-engaged-energy-balance-interventions-enough-for-extremely-vulnerable-populations
#9
Nancy E Schoenberg, Yelena N Tarasenko, Claire Snell-Rood
Well-documented associations between lifestyle behaviors and disease outcomes necessitate evidence-based health promotion interventions. To enhance potential efficacy and effectiveness, interventionists increasingly respond to community priorities, employ comprehensive theoretical frameworks, invest heavily to ensure cultural fit, implement evidence-based programming, and deploy research gold standards. We describe a project that followed all of these recommended strategies, but did not achieve desired outcomes...
January 17, 2018: Translational Behavioral Medicine
https://www.readbyqxmd.com/read/29351460/macrophage-dependent-impairment-of-%C3%AE-2-adrenergic-autoreceptor-function-in-sympathetic-neurons-from-doca-salt-but-not-high-fat-diet-induced-hypertensive-rats
#10
Ryan Kwun-Yee Mui, Roxanne Fernandes, Hannah Garver, Nico van Rooijen, James J Galligan
DOCA-salt and obesity-related hypertension are associated with inflammation and sympathetic nervous system hyperactivity. Prejunctional α2-adrenergic receptors (α2ARs) provide negative feedback to norepinephrine release from sympathetic nerves through inhibition of N-type Ca2+ channels. Increased neuronal norepinephrine release in DOCA-salt and obesity-related hypertension occurs through impaired α2AR signaling, however, the mechanisms involved are unclear. Mesenteric arteries are resistance arteries that receive sympathetic innervation from the superior mesenteric and celiac ganglia (SMCG)...
January 5, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29351145/recent-evidence-on-early-mobilization-in-critical-ill-patients
#11
Kristina Fuest, Stefan J Schaller
PURPOSE OF REVIEW: To examine the benefits of early mobilization and summarize the results of most recent clinical studies examining early mobilization in critically ill patients followed by a presentation of recent developments in the field. RECENT FINDINGS: Early mobilization of ICU patients, defined as mobilization within 72 h of ICU admission, is still uncommon. In medical and surgical critically ill patients, mobilization is well tolerated even in intubated patients...
January 17, 2018: Current Opinion in Anaesthesiology
https://www.readbyqxmd.com/read/29350999/sex-differences-in-abdominal-aortic-aneurysms
#12
Austin Boese, Lin Chang, Ke-Jie Yin, Y Eugene Chen, Jean-Pyo Lee, Milton H Hamblin
Abdominal aortic aneurysm (AAA) is a vascular disorder with a high case fatality rate in the instance of rupture. AAA is a multifactorial disease and the etiology is still not fully understood. AAA is more likely to occur in men, but women have a greater risk of rupture and worse prognosis. Women are reportedly protected against AAA possibly by premenopausal levels of estrogen and are, on average, diagnosed at older ages than men. Here, we review the current body of research on AAA pathophysiology in humans, animal models, and cultured cells, with an emphasis on sex differences and sex steroid hormone signaling...
January 19, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29350875/highly-dynamic-wintering-strategies-in-migratory-geese-coping-with-environmental-change
#13
Kevin Kuhlmann Clausen, Jesper Madsen, Fred Cottaar, Eckhart Kuijken, Christine Verscheure
When and where to move is a fundamental decision to migratory birds, and the fitness-related costs and benefits of migratory choices make them subject to strong selective forces. Site use and migration routes are outcomes of opportunities in the surrounding landscape, and the optimal migration strategy may be conservative or explorative depending on the variability of the environment occupied by the species. This study applies 25 years of resighting data to examine development in winter migration strategy of pink-footed geese divided among Denmark, the Netherlands and Belgium, and analyse potential drivers of strategy change as well as individuals' likelihood to break with migratory tradition...
January 19, 2018: Global Change Biology
https://www.readbyqxmd.com/read/29350833/platelets-kill-bacteria-by-bridging-innate-and-adaptive-immunity-via-pf4-and-fc%C3%AE-riia
#14
R Palankar, T P Kohler, K Krauel, J Wesche, S Hammerschmidt, A Greinacher
BACKGROUND: Activated platelets release the chemokine platelet factor 4 (PF4) stored in their granules. PF4 binds to polyanions (P) on bacteria, undergoes a conformational change and exposes neoepitopes. These neoepitopes induce production of anti-PF4/P antibodies. As PF4 binds to a variety of bacteria, anti-PF4/P IgG can bind and opsonize several bacterial species. OBJECTIVE: Here we investigated whether platelets are able to kill bacteria directly after recognizing anti-PF4/P IgG opsonized bacteria in the presence of PF4 via their FcγRIIA...
January 19, 2018: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29350422/potency-of-polycyclic-aromatic-hydrocarbons-pahs-in-chicken-and-japanese-quail-embryos
#15
Cynthia D Franci, Angelika Aleksieva, Emily Boulanger, Jonas Brandenburg, Theresa Johnston, Asya Malinova, Jessica A Head
Birds are receptors of concern for polycyclic aromatic hydrocarbons (PAHs), and yet limited data describing the relative potency of PAH congeners are available for avian species. Here, we determine embryonic LD50 values for 5 PAH congeners in chicken (Gallus gallus) and one PAH congener in Japanese quail (Coturnix japonica). Graded concentrations of each test compound were injected into the air cell of chicken or quail eggs before incubation. Embryos were monitored through development (quail) or hatching (chicken)...
January 19, 2018: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/29350417/hepatitis-c-virus-core-protein-induces-dysfunction-of-liver-sinusoidal-endothelial-cell-by-down-regulation-of-silent-information-regulator-1
#16
Li-Jie Sun, Jian-Wu Yu, Yu-Guang Shi, Xiao-Yu Zhang, Meng-Ni Shu, Mo-Yang Chen
Objective Hepatic fibrosis is a frequent feature of chronic hepatitis C virus (HCV) infection. Some evidence has suggested the potential role of silent information regulator 1 (SIRT1) in organ fibrosis. The aim of this study was to investigate the effect of HCV core protein on expression of SIRT1 of liver sinusoidal endothelial cell (LSEC) and function of LSEC. Methods LSECs were co-cultured with HepG2 cells or HepG2 cells expressing HCV core protein and LSECs cultured alone were used as controls. After co-culture, the activity and expression levels of mRNA and protein of SIRT1 in LSEC were detected by a SIRT1 fluorometric assay kit, real time-PCR (RT-PCR), Western blot, respectively...
January 19, 2018: Journal of Medical Virology
https://www.readbyqxmd.com/read/29350308/the-ppar%C3%AE-agonist-efatutazone-delays-invasive-progression-and-induces-differentiation-of-ductal-carcinoma-in-situ
#17
Virginie Ory, William B Kietzman, Jacob Boeckelman, Bhaskar V Kallakury, Anton Wellstein, Priscilla A Furth, Anna T Riegel
PURPOSE: Ductal carcinoma in situ (DCIS) is a pre-invasive lesion of the breast considered a precursor of invasive ductal carcinoma. This study aimed to determine whether activated PPARγ acts as a tumor suppressor in human DCIS progression. METHODS: We utilized the high-affinity PPARγ agonist, efatutazone, to activate endogenous PPARγ in a well-defined model for the progression of basal (triple negative) DCIS, MCFDCIS cells, cultured under 2D and 3D conditions...
January 19, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29349856/self-responsibility-rationing-and-treatment-decision-making-managing-moral-narratives-alongside-fiscal-reality-in-the-obesity-surgery-clinic
#18
Amanda Owen-Smith, Joanna Coast, Jenny L Donovan
BACKGROUND: Addressing the prevalence of severe obesity and its concomitant morbidities is widely acknowledged as one of the most pressing global health priorities. Nevertheless, a paucity of effective interventions and universal pressure on health-care budgets means that access to obesity treatments is often limited. Although health-care rationing can be conceived as a socially constructed process, little is known about how decisions emerge within the context of face-to-face doctor-patient interactions...
January 19, 2018: Health Expectations: An International Journal of Public Participation in Health Care and Health Policy
https://www.readbyqxmd.com/read/29349854/implementing-health-and-social-care-integration-in-scotland-renegotiating-new-partnerships-in-changing-cultures-of-care
#19
Charlotte Pearson, Nick Watson
Health and social care integration has been a long-term goal for successive governments in Scotland, culminating in the implementation of the recent Public Bodies (Joint Working) Scotland Act 2014. This laid down the foundations for the delegation of health and social care functions and resources to newly formed Integrated Joint Boards. It put in place demands for new ways of working and partnership planning. In this article, we explore the early implementation of this Act and how health and social care professionals and the third sector have begun to renegotiate their roles...
January 18, 2018: Health & Social Care in the Community
https://www.readbyqxmd.com/read/29349478/affinity-to-host-population-stimulates-physical-growth-in-adult-offspring-of-turkish-migrants-in-germany
#20
Aydan Özer, Christiane Scheffler
Because of political conflicts and climate change, migration will be increased worldwide and integration in host societies is a challenge also for migrants. We hypothesize that migrants, who take up the challenge in a new social environment are taller than migrants who do not pose this challenge. We analyze by a questionnaire possible social, nutritional and ethnic influencing factors to body height (BH) of adult offspring of Turkish migrants (n = 82, 39 males) aged from 18 to 34 years (mean age 24.6 years)...
January 18, 2018: Anthropologischer Anzeiger; Bericht über die Biologisch-anthropologische Literatur
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