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https://www.readbyqxmd.com/read/27890667/investigation-of-carbapenem-resistance-and-the-first-identification-of-klebsiella-pneumoniae-carbapenemase-kpc-enzyme-among-escherichia-coli-isolates-in-turkey-a-prospective-study
#1
Mert Ahmet Kuskucu, Asiye Karakullukcu, Mailihaba Ailiken, Barıs Otlu, Bilgul Mete, Gokhan Aygun
BACKGROUND: The aim of this study was to determine the presence of carbapenem resistance and carbapenemase production in Escherichia coli isolates from clinical samples in Turkey. METHODS: The prospective study included a total of 4052 Escherichia coli isolates collected from patients admitted to a hospital from March 2011 to May 2012. We used ertapenem disc for screening carbapenemase production, and the confirmation was performed by using Etest. The resistance mechanisms and genetic relatedness of the carbapenem resistant strains were investigated by using PCR (polymerase chain reaction) and pulsed-field gel electrophoresis (PFGE), respectively...
November 23, 2016: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/27872078/polymyxin-b-in-combination-with-rifampin-and-meropenem-against-polymyxin-b-resistant-kpc-producing-klebsiella-pneumoniae
#2
John Diep, David M Jacobs, Rajnikant Sharma, Jenna Covelli, Dana R Bowers, Thomas A Russo, Gauri G Rao
Safe and effective therapies are urgently needed to treat polymyxin-resistant KPC-producing K. pneumoniae and suppress the emergence of resistance. We investigated the pharmacodynamics of polymyxin B, rifampin, and meropenem alone and as polymyxin B-based double and triple combinations against KPC-producing K. pneumoniae The rate and extent of killing with polymyxin B (1-128mg/L), rifampin (2-16mg/L), and meropenem (10-120mg/L) were evaluated against polymyxin B-susceptible (PB(S)) and -resistant (PB(R)) clinical isolates using 48h static time-kills...
November 21, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27866944/occurrence-of-carbapenemase-producing-klebsiella-pneumoniae-and-escherichia-coli-in-the-european-survey-of-carbapenemase-producing-enterobacteriaceae-euscape-a-prospective-multinational-study
#3
Hajo Grundmann, Corinna Glasner, Barbara Albiger, David M Aanensen, Chris T Tomlinson, Arjana Tambić Andrasević, Rafael Cantón, Yehuda Carmeli, Alexander W Friedrich, Christian G Giske, Youri Glupczynski, Marek Gniadkowski, David M Livermore, Patrice Nordmann, Laurent Poirel, Gian M Rossolini, Harald Seifert, Alkiviadis Vatopoulos, Timothy Walsh, Neil Woodford, Dominique L Monnet
BACKGROUND: Gaps in the diagnostic capacity and heterogeneity of national surveillance and reporting standards in Europe make it difficult to contain carbapenemase-producing Enterobacteriaceae. We report the development of a consistent sampling framework and the results of the first structured survey on the occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in European hospitals. METHODS: National expert laboratories recruited hospitals with diagnostic capacities, who collected the first ten carbapenem non-susceptible clinical isolates of K pneumoniae or E coli and ten susceptible same-species comparator isolates and pertinent patient and hospital information...
November 17, 2016: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/27863949/imipenem-avibactam-a-novel-combination-for-the-rapid-detection-of-carbapenemase-activity-in-enterobacteriaceae-and-acinetobacter-baumannii-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#4
Marina Oviaño, Germán Bou
In the present study, we propose a novel matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for detecting carbapenemase-producing Enterobacteriaceae and Acinetobacter baumannii. For this, we analyzed a series of 131 isolates. Among them, a total of 115 Enterobacteriaceae: 79 of them carrying a carbapenemase enzyme (15blaKPC, 7blaNDM, 11blaIMP, 12blaVIM, and 34blaOXA-48) and 16 A. baumannii isolates: 15 of them carrying carbapenemases (10blaOXA-23, 2blaOXA-58, 2blaOXA-24, and 1blaOXA-237)...
October 18, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27846056/mother-to-child-transmission-of-kpc-carbapenemase-producing-klebsiella-pneumoniae-at-birth
#5
Paolo Bonfanti, Roberto Bellù, Luigi Principe, Ilaria Caramma, Manuela Condò, Tommaso Giani, Gian Maria Rossolini, Francesco Luzzaro
We report on a mother-to-child transmission of KPC carbapenemase-producing Klebsiella pneumoniae at birth followed by subsequent cases in the NICU. Molecular analysis of isolates showed production of KPC-3 enzyme. The only potential risk factor identified for the mother was previous activity as a caregiver. Present findings suggest consideration of pro-active surveillance in pregnant women with risk factors for colonization.
November 11, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/27835839/characterisation-of-carbapenem-resistant-enterobacteriaceae-from-the-southwestern-ohio-northern-kentucky-and-southeastern-indiana-region
#6
Joel E Mortensen, Barbara DeBurger, Eleanor A Powell, Jeanna DiFranco-Fisher, Laura Koeth, Scott J Weissman
Although carbapenem-resistant Enterobacteriaceae (CRE) have been recognised worldwide, there are important gaps in regional prevalence data. This study sought to determine the occurrence and type of CRE in the Cincinnati tri-state region. Clinical microbiology laboratories in the region screened patient stool and rectal swab samples using chromogenic screening agar. Isolates of Enterobacteriaceae resistant to carbapenems were characterised using molecular methods. Area institutions screened 1939 patient samples...
December 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27834322/a-structural-epidemiological-genetic-overview-of-klebsiella-pneumoniae-carbapenemases-kpcs
#7
REVIEW
C H Swathi, Rosy Chikala, K S Ratnakar, V Sritharan
Klebsiella pneumoniae carbapenemases (KPCs) are plasmid encoded carbapenem hydrolyzing enzymes which have the potential to spread widely through gene transfer. The instability of upstream region of blaKPC accelerates emergence of different isoforms. Routine antibiotic susceptibility testing failed to detect KPC producers and some commercial kits have been launched for early identification of KPC producers. Notable among the drugs under development against KPC are mostly derivatives of polymixin; ß-lactamase inhibitor NXL104 with combination of oxyimino cephalosporin as well as with ceftazidime; a novel tricyclic carbapenem, LK-157, potentially useful against class A and class C enzymes; BLI-489-a bicyclic penem derivative; PTK-0796, a tetracycline derivative and ACHN-490...
July 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27832961/emergence-of-colistin-resistance-in-the-largest-university-hospital-complex-of-s%C3%A3-o-paulo-brazil-over-five-years
#8
Flávia Rossi, Raquel Girardello, Ana Paula Cury, Thais Sabato Romano Di Gioia, João Nóbrega de Almeida, Alberto José da Silva Duarte
Colistin resistance involving Gram-negative bacilli infections is a challenge for health institutions around of the world. Carbapenem-resistance among these isolates makes colistin the last therapeutic option for this treatment. Colistin resistance among Enterobacteriaceae, Acinetobacter spp., and Pseudomonas spp. was evaluated between 2010 and 2014 years, at Hospital das Clínicas, São Paulo, Brazil. Over five years 1346 (4.0%) colistin resistant Gram-negative bacilli were evaluated. Enterobacteriaceae was the most frequent (86...
November 7, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27825605/antimicrobial-resistance-in-enterobacteriaceae-in-brazil-focus-on-%C3%AE-lactams-and-polymyxins
#9
REVIEW
Jorge Luiz Mello Sampaio, Ana Cristina Gales
During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96...
October 25, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27821357/evaluation-of-meat-fruit-and-vegetables-from-retail-stores-in-five-united-kingdom-regions-as-sources-of-extended-spectrum-beta-lactamase-esbl-producing-and-carbapenem-resistant-escherichia-coli
#10
L P Randall, M P Lodge, N C Elviss, F L Lemma, K L Hopkins, C J Teale, N Woodford
We determined the prevalence and types of extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Escherichia coli in raw retail beef, chicken, pork, fruit and vegetables in five UK regions in 2013-14. Raw meat (n=397), and fruit and vegetable samples (n=400) were purchased from retail stores in London, East Anglia, North West England, Scotland and Wales. Samples were tested for the presence of ESBL-producing E. coli by plating enriched samples on CHROMagar CTX and CHROMagar ESBL, for AmpC-type E...
October 29, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27818808/persistent-bacteremia-from-pseudomonas-aeruginosa-with-in-vitro-resistance-to-the-novel-antibiotics-ceftolozane-tazobactam-and-ceftazidime-avibactam
#11
Louie Mar Gangcuangco, Patricia Clark, Cynthia Stewart, Goran Miljkovic, Zane K Saul
Ceftazidime-avibactam and ceftolozane-tazobactam are new antimicrobials with activity against multidrug-resistant Pseudomonas aeruginosa. We present the first case of persistent P. aeruginosa bacteremia with in vitro resistance to these novel antimicrobials. A 68-year-old man with newly diagnosed follicular lymphoma was admitted to the medical intensive care unit for sepsis and right lower extremity cellulitis. The patient was placed empirically on vancomycin and piperacillin-tazobactam. Blood cultures from Day 1 of hospitalization grew P...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27815135/klebsiella-pneumoniae-blakpc-3-nosocomial-epidemic-bayesian-and-evolutionary-analysis
#12
Silvia Angeletti, Alessandra Lo Presti, Eleonora Cella, Marta Fogolari, Lucia De Florio, Etleva Dedej, Aletheia Blasi, Teresa Milano, Stefano Pascarella, Raffaele Antonelli Incalzi, Roberto Coppola, Giordano Dicuonzo, Massimo Ciccozzi
K. pneumoniae isolates carrying blaKPC-3 gene were collected to perform Bayesian phylogenetic and selective pressure analysis and to apply homology modeling to the KPC-3 protein. A dataset of 44 blakpc-3 gene sequences from clinical isolates of K. pneumoniae was used for Bayesian phylogenetic, selective pressure analysis and homology modeling. The mean evolutionary rate for blakpc-3 gene was 2.67×10(-3) substitution/site/year (95% HPD: 3.4×10(-4-)5.59×10(-)(3)). The root of the Bayesian tree dated back to the year 2011 (95% HPD: 2007-2012)...
November 1, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27812488/identification-of-klebsiella-pneumoniae-carbapenemase-producing-klebsiella-oxytoca-in-clinical-isolates-in-tehran-hospitals-iran-by-chromogenic-medium-and-molecular-methods
#13
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani
OBJECTIVES: Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as Klebsiella oxytoca. This study aimed to investigate and identify KPC-producing K. oxytoca isolates using molecular and phenotypic methods. METHODS: A total of 75 isolates of K. oxytoca were isolated from various clinical samples, and were verified as K. oxytoca after performing standard microbiological tests and using a polymerase chain reaction (PCR) method...
October 2016: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/27812159/enterobacteriaceae-isolated-from-the-river-danube-antibiotic-resistances-with-a-focus-on-the-presence-of-esbl-and-carbapenemases
#14
Clemens Kittinger, Michaela Lipp, Bettina Folli, Alexander Kirschner, Rita Baumert, Herbert Galler, Andrea J Grisold, Josefa Luxner, Melanie Weissenbacher, Andreas H Farnleitner, Gernot Zarfel
In a clinical setting it seems to be normal these days that a relevant proportion or even the majority of different bacterial species has already one or more acquired antibiotic resistances. Unfortunately, the overuse of antibiotics for livestock breeding and medicine has also altered the wild-type resistance profiles of many bacterial species in different environmental settings. As a matter of fact, getting in contact with resistant bacteria is no longer restricted to hospitals. Beside food and food production, the aquatic environment might also play an important role as reservoir and carrier...
2016: PloS One
https://www.readbyqxmd.com/read/27811089/draft-genome-sequence-of-a-hospital-associated-clone-of-klebsiella-pneumoniae-st340-cc258-coproducing-rmtg-and-kpc-2-isolated-from-a-pediatric-patient
#15
Louise Cerdeira, Miriam R Fernandes, Gabriela R Francisco, Maria Fernanda C Bueno, Susan Ienne, Tiago A Souza, Doroti de Oliveira Garcia, Nilton Lincopan
We report here the draft genome sequence of a Klebsiella pneumoniae strain 1194/11, belonging to the hospital-associated sequence type 340 (ST340; clonal complex CC258), isolated from a catheter tip culture from a pediatric patient. The multidrug-resistant strain coproduced the 16S rRNA methyltransferase rRNA RmtG and β-lactamases KPC-2 and CTX-M-15.
November 3, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27809803/a-prospective-study-of-treatment-of-carbapenem-resistant-enterobacteriaceae-infections-and-risk-factors-associated-with-outcome
#16
Claudia M D de Maio Carrilho, Larissa Marques de Oliveira, Juliana Gaudereto, Jamile S Perozin, Mariana Ragassi Urbano, Carlos H Camargo, Cintia M C Grion, Anna Sara S Levin, Silvia F Costa
BACKGROUND: To describe the clinical and microbiological data of carbapenem-resistant Enterobacteriaceae (CRE) infections, the treatment used, hospital- and infection-related mortality, and risk factors for death. METHODS: A prospective cohort conducted from March 2011 to December 2012. Clinical, demographic, and microbiological data such as in vitro sensitivity, clonality, carbapenemase gene mortality related to infection, and overall mortality were evaluated. Data were analyzed using Epi Info version 7...
November 3, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27802107/clonal-spread-of-colistin-resistant-klebsiella-pneumoniae-coproducing-kpc-and-vim-carbapenemases-in-neonates-at-a-tunisian-university-hospital
#17
Hajer Battikh, Chiraz Harchay, Amal Dekhili, Khaoula Khazar, Fehima Kechrid, Meriem Zribi, Afef Masmoudi, Chedlia Fendri
In this study, we have attempted to report the first clonal spread of colistin-resistant Klebsiella pneumoniae coproducing KPC and VIM carbapenemases in the neonatal unit of Rabta Teaching Hospital of Tunis (Tunisia). This retrospective study was performed from January 1, 2014 to December 31, 2014 in the Microbiology Laboratory at the Rabta University Hospital of Tunis. Twenty-one nonreplicate colistin-resistant K. pneumoniae were isolated from 19 patients hospitalized in the neonatal unit and 2 patients in the adult intensive care unit (ICU)...
November 1, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27800140/detection-of-kpc-carbapenemase-in-pseudomonas-aeruginosa-isolated-from-clinical-samples-using-modified-hodge-test-and-boronic-acid-phenotypic-methods-and-their-comparison-with-the-polymerase-chain-reaction
#18
Saeed Falahat, Mana Shojapour, Abdorrahim Sadeghi
BACKGROUND: Bacterial resistance to antibiotics has become a major source of concern for public health. Pseudomonas aeruginosa strains are important opportunistic pathogens. These bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics. OBJECTIVES: The present study was performed to evaluate the frequency of KPC in P. aeruginosa isolated from clinical samples of educational hospitals of Arak University of Medical Sciences, using the mentioned phenotypic and genotypic methods...
September 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27798215/phenotypic-detection-of-the-cfia-metallo-%C3%AE-lactamase-in-bacteroides-fragilis-with-the-meropenem-edta-double-ended-etest-and-the-rosco-kpc-mbl-confirm-kit
#19
Simon A Schwensen, Ziyap Acar, Thomas V Sydenham, Åsa C Johansson, Ulrik S Justesen
OBJECTIVES: To investigate the performance of the meropenem and imipenem double-ended Etest ± EDTA and the tablet-based (meropenem and meropenem + dipicolinic acid) KPC/MBL Confirm Kit to detect cfiA metallo-β-lactamase (MBL) in Bacteroides fragilis. METHODS: Well-characterized B. fragilis isolates, most from previously published studies, harbouring the cfiA gene and covering a wide range of meropenem MICs were included (n = 21). RESULTS: The imipenem double-ended Etest showed an indeterminate result in 95% of the included isolates with the cfiA gene (20 of 21), whereas the meropenem double-ended Etest gave an MIC ratio ≥8 (positive test) with all the isolates...
October 20, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27797936/il-6-and-pd-l1-antibody-blockade-combination-therapy-reduces-tumour-progression-in-murine-models-of-pancreatic-cancer
#20
Thomas A Mace, Reena Shakya, Jason R Pitarresi, Benjamin Swanson, Christopher W McQuinn, Shannon Loftus, Emily Nordquist, Zobeida Cruz-Monserrate, Lianbo Yu, Gregory Young, Xiaoling Zhong, Teresa A Zimmers, Michael C Ostrowski, Thomas Ludwig, Mark Bloomston, Tanios Bekaii-Saab, Gregory B Lesinski
OBJECTIVE: Limited efficacy of immune checkpoint inhibitors in pancreatic ductal adenocarcinoma (PDAC) has prompted investigation into combination therapy. We hypothesised that interleukin 6 (IL-6) blockade would modulate immunological features of PDAC and enhance the efficacy of anti-programmed death-1-ligand 1 (PD-L1) checkpoint inhibitor therapy. DESIGN: Transcription profiles and IL-6 secretion from primary patient-derived pancreatic stellate cells (PSCs) were analyzed via Nanostring and immunohistochemistry, respectively...
October 21, 2016: Gut
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