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https://www.readbyqxmd.com/read/28429335/imipenem-resistance-in-serratia-marcescens-is-mediated-by-plasmid-expression-of-kpc-2
#1
W-Q Su, Y-Q Zhu, N-M Deng, L Li
OBJECTIVE: Imipenem is a broad-spectrum carbapenem antibiotic with applications against severe bacterial infections. Here, we describe the identification of imipenem-resistant Serratia marcescens in our hospital and the role of plasmid-mediated KPC-2 expression in imipenem resistance. MATERIALS AND METHODS: We used the modified Hodge test to detect carbapenemase produced in imipenem-resistant strains. RESULTS: His resistance can be transferred to E...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28425875/occurrence-of-qnrb1-and-qnrb12-genes-mutation-in-gyra-and-ramr-and-expression-of-efflux-pumps-in-isolates-of-klebsiella-pneumoniae-carriers-of-blakpc-2
#2
Alexsandra Maria Lima Scavuzzi, Maria Amélia Vieira Maciel, Heloísa Ramos Lacerda de Melo, Luiz Carlos Alves, Fábio André Brayner, Ana Catarina Souza Lopes
PURPOSE: The occurrence of quinolone-resistance genes (qnrA, qnrB and qnrS), the presence of mutations in gyrA, gyrB and parC, as well as the expression of efflux pumps (acrB and acrF) and mutations in the gene ramR. METHODOLOGY: Were investigated in 30 blaKPC-2-positive isolates of Klebsiella pneumoniae taken from infection and colonization in hospital patients from Recife-PE, Brazil. The detection of the qnr, acrB and acrF genes and analysis of the mutations in ramR and the quinolone-resistance-determining regions of gyrA, gyrB and parC were performed by PCR followed by DNA sequencing...
April 21, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28414315/the-emt-activator-zeb1-is-a-key-factor-for-cell-plasticity-and-promotes-metastasis-in-pancreatic-cancer
#3
Angela M Krebs, Julia Mitschke, María Lasierra Losada, Otto Schmalhofer, Melanie Boerries, Hauke Busch, Martin Boettcher, Dimitrios Mougiakakos, Wilfried Reichardt, Peter Bronsert, Valerie G Brunton, Christian Pilarsky, Thomas H Winkler, Simone Brabletz, Marc P Stemmler, Thomas Brabletz
Metastasis is the major cause of cancer-associated death. Partial activation of the epithelial-to-mesenchymal transition program (partial EMT) was considered a major driver of tumour progression from initiation to metastasis. However, the role of EMT in promoting metastasis has recently been challenged, in particular concerning effects of the Snail and Twist EMT transcription factors (EMT-TFs) in pancreatic cancer. In contrast, we show here that in the same pancreatic cancer model, driven by Pdx1-cre-mediated activation of mutant Kras and p53 (KPC model), the EMT-TF Zeb1 is a key factor for the formation of precursor lesions, invasion and notably metastasis...
April 17, 2017: Nature Cell Biology
https://www.readbyqxmd.com/read/28411078/performance-of-the-xpert-%C3%A2-carba-r-v2-in-the-daily-workflow-of-a-hygiene-unit-in-a-country-with-a-low-prevalence-of-carbapenemase-producing-enterobacteriaceae
#4
Yannick Hoyos, Souad Ouzani, Laurent Dortet, Nicolas Fortineau, Thierry Naas
Early detection of patients colonised with carbapenemase-producing Enterobacteriaceae (CPE) is crucial for implementing proper infection control measures. Here we evaluated the biological performance of the Xpert(®) Carba-R v2 (Cepheid) in the daily workflow of a hygiene unit in a country with a low CPE prevalence. Patients repatriated from countries known for high CPE prevalence or contact patients of a known CPE carrier were targeted as being 'high-risk patients' for CPE carriage. Between September 2015 and March 2016, 241 'high-risk patients' for CPE carriage were screened using the Xpert(®) Carba-R v2 and by plating on chromID(®) CARBA Smart medium (bioMérieux) with and without ertapenem-containing enrichment culture for 24 h...
April 11, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28404680/development-and-validation-of-a-lateral-flow-immunoassay-for-the-rapid-detection-of-ndm-producing-enterobacteriaceae
#5
Hervé Boutal, Thierry Naas, Karine Devilliers, Saoussen Oueslati, Laurent Dortet, Sandrine Bernabeu, Stéphanie Simon, Hervé Volland
The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flow immunoassay (LFIA, called NDM LFIA) for rapid and reliable detection of NDM-like carbapenemase-producing Enterobacteriaceae from culture colonies.We evaluated the NDM LFIA using 175 reference enterobacterial isolates with characterized ß-lactamase gene content and 74 non-duplicate consecutive carbapenem-resistant clinical isolates referred for expertise to the French National Reference Center (NRC) for antibiotic resistance during a one-week period (in June 2016)...
April 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28402500/evaluation-of-the-aid-carbapenemase-line-probe-assay-for-rapid-detection-and-identification-of-carbapenemase-genes-in-gram-negative-bacilli
#6
Guido V Bloemberg, Andrea Braun-Kiewnick, Jan Tedrup, Carla Meijerink, Elena Durer, Claudia Ritter, Peter M Keller, Michael Hombach
Objectives: This study evaluated the AID carbapenemase line probe assay (LPA) for the detection and identification of carbapenem resistance genes in Enterobacteriaceae and other Gram-negative bacilli (GNB) using bacterial cultures and DNA extracts directly from patient urine samples. Methods: The AID carbapenemase LPA detects 13 different carbapenemase genes. Test probe accuracy was verified for using clinical Enterobacteriaceae isolates harbouring bla KPC , bla VIM , bla NDM , bla GIM , bla AIM , bla SPM , bla IMP and bla OXA-48 and a well-characterized set of Escherichia coli DH5α strains transformed with the vector plasmid pUC57- kan harbouring bla BIC , bla SIM , bla DIM , bla IMI-3 , bla IMI-1 and bla NMC-A ...
April 11, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28397615/modifiable-risk-factors-for-the-spread-of-klebsiella-pneumoniae-carbapenemase-producing-enterobacteriaceae-among-long-term-acute-care-hospital-patients
#7
Koh Okamoto, Michael Y Lin, Manon Haverkate, Karen Lolans, Nicholas M Moore, Shayna Weiner, Rosie D Lyles, Donald Blom, Yoona Rhee, Sarah Kemble, Louis Fogg, David W Hines, Robert A Weinstein, Mary K Hayden
OBJECTIVE To identify modifiable risk factors for acquisition of Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae (KPC) colonization among long-term acute-care hospital (LTACH) patients. DESIGN Multicenter, matched case-control study. SETTING Four LTACHs in Chicago, Illinois. PARTICIPANTS Each case patient included in this study had a KPC-negative rectal surveillance culture on admission followed by a KPC-positive surveillance culture later in the hospital stay. Each matched control patient had a KPC-negative rectal surveillance culture on admission and no KPC isolated during the hospital stay...
April 11, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28397613/know-thy-self-know-thy-enemy-a-current-survey-and-a-forecast-for-kpc-producing-klebsiella-pneumoniae-resistance-among-inpatients-in-southern-brazil
#8
Leandro Reus Rodrigues Perez
No abstract text is available yet for this article.
April 11, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28396547/resistance-to-ceftazidime-avibactam-in-klebsiella-pneumoniae-due-to-porin-mutations-and-the-increased-expression-of-kpc-3
#9
Romney M Humphries, Peera Hemarajata
We reported the first clinical case of a ceftazidime-avibactam resistant KPC-3-producing Klebsiella pneumoniae (1), from a patient with no prior history of ceftazidime-avibactam therapy.….
April 10, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28389354/treating-complicated-carbapenem-resistant-enterobacteriaceae-infections-with-ceftazidime-avibactam-a-retrospective-study-with-molecular-strain-characterisation
#10
Fiorella Krapp, Jennifer L Grant, Sarah H Sutton, Egon A Ozer, Viktorija O Barr
Ceftazidime/avibactam (CAZ/AVI) is the first antimicrobial agent with activity against carbapenem-resistant Enterobacteriaceae (CRE) approved by the US Food and Drug Administration (FDA). Notably, human clinical outcome data for this indication are limited. Therefore, a retrospective study was performed to evaluate the clinical outcomes and bacterial genomic characteristics of patients hospitalised at a tertiary medical centre with CRE infections treated for the first time with CAZ/AVI. From a total of 44 patients with CRE infections, 6 patients were treated with CAZ/AVI...
April 4, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28389093/risk-factors-for-kpc-producing-enterobacteriaceae-acquisition-and-infection-in-a-healthcare-setting-with-possible-local-transmission-a-case-control-study
#11
K M Cronin, Y S Poy Lorenzo, M E Olenski, A E Bloch, K Visvanathan, M J Waters, K L Buising
BACKGROUND: Reports of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kp) in Australia were previously uncommon, with cases imported sporadically by travellers from higher prevalence countries. AIM: The study institution reported the first outbreak of KPC-Kp in Australia. The aim of this study was to identify risk factors for KPC-Kp colonization and infection using a matched case-control study. METHODS: The study included all hospitalized patients with KPC-Kp colonization or infection from January 2012 to September 2015...
February 16, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28388065/molecular-basis-of-substrate-recognition-and-product-release-by-the-klebsiella-pneumoniae-carbapenemase-kpc-2
#12
Orville Antonio Pemberton, Xiujun Zhang, Yu Chen
Carbapenem-resistant Enterobacteriaceae (CRE) are resistant to most β-lactam antibiotics due to production of the KPC-2 class A β-lactamase. Here we present the first product complex crystal structures of KPC-2 with β-lactam antibiotics, containing hydrolyzed cefotaxime and faropenem. They provide experimental insights into substrate recognition by KPC-2 and its unique cephalosporinase/carbapenemase activity. These structures also represent the first product complexes for a wild type serine β-lactamase, elucidating the product release mechanism of these enzymes in general...
April 7, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28384185/evaluation-of-a-modified-meropenem-hydrolysis-assay-on-a-large-cohort-of-kpc-and-vim-carbapenemase-producing-enterobacteriaceae
#13
Adriana Calderaro, Mirko Buttrini, Maddalena Piergianni, Sara Montecchini, Monica Martinelli, Silvia Covan, Giovanna Piccolo, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Carbapenem-resistant Enterobacteriaceae (CRE) have spread globally and represent a serious and growing threat to public health. The introduction of rapid and sensitive methods for the detection of carbapenemase-producing bacteria is of increasing importance. The carbapenemase production can be detected using non-molecular methods (such as the modified Hodge test, the synergy test, the Carba NP test and the antibiotic hydrolysis assays) and DNA-based methods. In this study, we propose a modified version of a previously described meropenem hydrolysis assay (MHA) by MALDI-TOF MS for the phenotypic detection in 2h of carbapenemase-producing Enterobacteriaceae...
2017: PloS One
https://www.readbyqxmd.com/read/28380016/carbapenemase-producing-enterobacteriaceae-recovered-from-a-spanish-river-ecosystem
#14
Núria Piedra-Carrasco, Anna Fàbrega, William Calero-Cáceres, Thais Cornejo-Sánchez, Maryury Brown-Jaque, Alba Mir-Cros, Maite Muniesa, Juan José González-López
The increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. The dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. The objective of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) collected from a river ecosystem in the Barcelona metropolitan area (Spain). Identification of β-lactamases and other resistance determinants was determined as was the antimicrobial susceptibility profile...
2017: PloS One
https://www.readbyqxmd.com/read/28378066/genetic-background-of-%C3%AE-lactamases-in-enterobacteriaceae-isolates-from-environmental-samples
#15
Daniele V de Oliveira, Luciana S Nunes, Afonso Luís Barth, Sueli T Van Der Sand
The prevalence of β-lactamase-producing Enterobacteriaceae has increased worldwide. Although antibiotic-resistant bacteria are usually associated with hospitals, there are a growing number of reports of resistant bacteria in other environments. Concern about resistant microorganisms outside the hospital setting highlights the need to investigate mechanisms of antibiotic resistance in isolates collected from the environment. The present study evaluated the resistance mechanism to β-lactam antibiotics in 40 isolates from hospital sewage and surface water from the Dilúvio Stream, Porto Alegre City, Southern Brazil...
April 4, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28373185/enhanced-klebsiella-pneumoniae-carbapenemase-kpc-expression-from-a-novel-tn4401-deletion
#16
Anita Cheruvanky, Nicole Stoesser, Anna E Sheppard, Derrick W Crook, Paul S Hoffman, Erin Weddle, Joanne Carroll, Costi D Sifri, Weidong Chai, Katie Barry, Girija Ramakrishnan, Amy J Mathers
The Klebsiella pneumoniae carbapenemase gene (blaKPC) is typically located within the mobile transposon Tn4401 Enhanced KPC expression has been associated with deletions in the putative promoter region upstream of blaKPC Illumina sequences from blaKPC-positive clinical isolates from a single institution were mapped to a Tn4401b reference sequence, which carries no deletions. The novel isoform Tn4401h (188bp deletion [lsqb]between istB and blaKPC[rsqb]) was present in 14% (39/281) clinical isolates. MICs for Escherichia coli strains containing plasmids with Tn4401a and Tn4401h were more resistant to meropenem (≥16, ≥16), ertapenem (≥8, 4) and cefepime (≥64, 4) than E...
April 3, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28369469/prospective-evaluation-of-the-okn-k-set-assay-a-new-multiplex-immunochromatographic-test-for-the-rapid-detection-of-oxa-48-like-kpc-and-ndm-carbapenemases
#17
Youri Glupczynski, Agnès Jousset, Stéphanie Evrard, Rémy A Bonnin, Te-Din Huang, Laurent Dortet, Pierre Bogaerts, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests capable of identifying carbapenemase producers. Here, we assessed the performance of a new multiplex lateral flow assay (OKN K -SeT) for the rapid detection of OXA-48-like, KPC and NDM carbapenemase-producing Enterobacteriaceae from culture colonies. Methods: Two hundred collection isolates with characterized β-lactamase content and 183 non-duplicate consecutive isolates referred to two National Reference Centres over a 2 month period in 2016 were used to evaluate the OKN K -SeT assay...
March 22, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28349292/in-vitro-and-in-vivo-comparison-of-gemcitabine-and-the-gemcitabine-analog-1-2-deoxy-2-fluoroarabinofuranosyl-cytosine-fac-in-human-orthotopic-and-genetically-modified-mouse-pancreatic-cancer-models
#18
James Russell, Nagavarakishore Pillarsetty, Robin M Kramer, Paul B Romesser, Pooja Desai, Adriana Haimovitz-Friedman, Maeve A Lowery, John L Humm
PURPOSE: Although gemcitabine is a mainstay of pancreatic cancer therapy, it is only moderately effective, and it would be desirable to measure drug uptake in patients. 1-(2'-deoxy-2'-fluoroarabinofuranosyl) cytosine (FAC), is an analog of gemcitabine, and when labeled with F-18, it may be a potential surrogate PET tracer for the drug. PROCEDURES: [(18)F]FAC was synthesized to a radiochemical purity of >96 %. The human tumor lines AsPC1, BxPC3, Capan-1, Panc1, and MiaPaca2 were grown orthotopically in nude mice...
March 27, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28340557/development-of-selective-medium-for-imp-type-carbapenemase-producing-enterobacteriaceae-in-stool-specimens
#19
Norihisa Yamamoto, Ryuji Kawahara, Yukihiro Akeda, Rathina Kumar Shanmugakani, Hisao Yoshida, Hideharu Hagiya, Naohiro Hara, Isao Nishi, Satomi Yukawa, Rumiko Asada, Yumi Sasaki, Kazuhiro Maeda, Noriko Sakamoto, Shigeyuki Hamada, Kazunori Tomono
BACKGROUND: Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. METHODS: Fifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO4) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO4)...
March 24, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28333363/evaluation-of-the-%C3%AE-carba%C3%A2-test-a-colorimetric-test-for-the-rapid-detection-of-carbapenemase-activity-in-gram-negative-bacilli
#20
Sandrine Bernabeu, Laurent Dortet, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated a novel colorimetric test (the β-CARBA™ test; Bio-Rad) to detect carbapenemase-producing Gram-negative bacilli from cultured colonies. Methods: The performance of the β-CARBA™ test was compared with that of the Carba NP test (or the CarbAcineto NP test) and RAPIDEC ® CARBA NP (bioMérieux) using a collection of 290 isolates with characterized β-lactamase content...
March 10, 2017: Journal of Antimicrobial Chemotherapy
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