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JOURNAL ARTICLE
Elsa D Ibáñez-Prada, Ingrid G Bustos, Enrique Gamboa-Silva, Diego F Josa, Lina Mendez, Yuli V Fuentes, Cristian C Serrano-Mayorga, Oscar Baron, Alejandra Ruiz-Cuartas, Edwin Silva, Louise M Judd, Taylor Harshegyi, Hector F Africano, Juan Urrego-Reyes, Claudia C Beltran, Sebastian Medina, Rafael Leal, Andrew J Stewardson, Kelly L Wyres, Jane Hawkey, Luis Felipe Reyes
In this study, the genetic differences and clinical impact of the carbapenemase-encoding genes among the community and healthcare-acquired infections were assessed. This retrospective, multicenter cohort study was conducted in Colombia and included patients infected with carbapenem-resistant Gram-negative rods between 2017 and 2021. Carbapenem resistance was identified by Vitek, and carbapenemase-encoding genes were identified by whole-genome sequencing (WGS) to classify the alleles and sequence types (STs)...
April 17, 2024: Microbiology Spectrum
#2
JOURNAL ARTICLE
Wei Zhang, Yufei Wang, Kaiying Wang, Jinhui Li, Jia Liu, Shulei Li, Lijie Song, Chunchen Liao, Xiaoli Yang, Peng Li, Xiong Liu
BACKGROUND: Urinary tract infection (UTI) associated with Klebsiella pneumoniae poses a serious threat for inpatients. This study aimed to describe the genomic characteristics of K. pneumoniae causing UTI in a tertiary-care hospital in Beijing, China. METHODS: A total of 20 K. pneumoniae strains collected from 2020 to 2021 were performed whole-genome sequencing. The Antibiotic susceptibility of 19 common antimicrobial agents was tested against all strains. The multi-locus sequence types (MLSTs) and serotypes were determined from the WGS data...
2024: Infection and Drug Resistance
#3
JOURNAL ARTICLE
Nanfei Wang, Xuan Zheng, Sebastian Leptihn, Yue Li, Heng Cai, Piaopiao Zhang, Wenhao Wu, Yunsong Yu, Xiaoting Hua
Research on megaplasmids that contribute to the spread of antimicrobial resistance (AMR) in Pseudomonas aeruginosa strains has grown in recent years due to the now widely used technologies allowing long-read sequencing. Here, we systematically analyzed distinct and consistent genetic characteristics of megaplasmids found in P. aeruginosa . Our data provide information on their phylogenetic distribution and hypotheses tracing the potential evolutionary paths of megaplasmids. Most of the megaplasmids we found belong to the IncP-2-type, with conserved and syntenic genetic backbones carrying modules of genes associated with chemotaxis apparatus, tellurite resistance and plasmid replication, segregation, and transmission...
December 2024: Computational and Structural Biotechnology Journal
#4
JOURNAL ARTICLE
Ali Aydin, Ali Anil Suleymanoglu, Abzal Abdramanov, Peter Paulsen, Emek Dumen
Antimicrobial resistance is one of the major public health problems worldwide. This study aimed to detect the presence of extended-spectrum β-lactamase-(ESBL-)producing Escherichia ( E. ) coli in chicken meat in Istanbul, Türkiye. Raw chicken meat samples ( n = 208) were collected from different sale points and analyzed for ESBL-producing E. coli . In total, 101 (48.5%) isolates were confirmed as E. coli by PCR, of which 80/101 (79.2%) demonstrated multiple antibiotic resistance. Resistance against amoxicillin-clavulanic acid was most frequent (87...
April 7, 2024: Foods (Basel, Switzerland)
#5
JOURNAL ARTICLE
Mark G Wise, James A Karlowsky, Naglaa Mohamed, Elizabeth D Hermsen, Shweta Kamat, Andy Townsend, Adrian Brink, Alex Soriano, David L Paterson, Luke S P Moore, Daniel F Sahm
OBJECTIVES: To report trends in carbapenem resistance and difficult-to-treat resistance (DTR) among clinical isolates of Gram-negative priority pathogens collected by the ATLAS global surveillance program from 2018 to 2022. METHODS: Reference broth microdilution testing was performed in a central laboratory for 79,214 Enterobacterales, 30,504 Pseudomonas aeruginosa, and 13,500 Acinetobacter baumannii-calcoaceticus complex isolates collected by a constant set of 157 medical centres in 49 countries in Asia Pacific (APAC), Europe (EUR), Latin America (LATAM), Middle East-Africa (MEA), and North America (NA) regions...
April 10, 2024: Journal of Global Antimicrobial Resistance
#6
Yingying Shen, Junmin Cao, Tonglin Hu, Xiawan Yang, Yuechao Zhao, Yiping Shen, Baodong Ye, Yunsong Yu, Dijiong Wu
BACKGROUND: Carbapenem-resistant P. aeruginosa (CRPA) is a common hospital-acquired bacterium. It exhibits high resistance to many antibiotics, including ceftazidime/avibactam and cefteolozane/tazobactam . The presence of carbapenem-resistant genes and co-existence Klebsiella pneumoniae carbapenemase (KPC) and metallo-β-lactamases (MBLs) further inactivated all β-lactams. Understanding the resistance genes of CRPA can help in uncovering the resistance mechanism and guiding anti-infective treatment...
2024: Infection and Drug Resistance
#7
JOURNAL ARTICLE
Yu-Shan Huang, Jia-Ling Yang, Jann-Tay Wang, Wang-Huei Sheng, Chia-Jui Yang, Yu-Chung Chuang, Shan-Chwen Chang
BACKGROUND: Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a substantial healthcare challenge. This study assessed the in vitro efficacy of selected antibiotic combinations against CRKP infections. METHODS: Our research involved the evaluation of 40 clinical isolates of CRKP, with half expressing Klebsiella pneumoniae carbapenemase (KPC) and half producing Metallo-β-lactamase (MBL), two key enzymes contributing to carbapenem resistance. We determined the minimum inhibitory concentrations (MICs) of four antibiotics: eravacycline, tigecycline, polymyxin-B, and ceftazidime/avibactam...
March 27, 2024: Journal of Infection and Public Health
#8
JOURNAL ARTICLE
Ge Li, Chengyu Liao, Jiangzhi Chen, Zuwei Wang, Shuncang Zhu, Jianlin Lai, Qiaowei Li, Yinhao Chen, Dihan Wu, Jianbo Li, Yi Huang, Yifeng Tian, Yanling Chen, Shi Chen
Induction of ferroptosis can inhibit cancer cells in vitro, however, the role of ferroptosis in treatment in vivo is controversial. The immunosuppressive cells activated by the ferroptotic tumor cells can promote the growth of residual tumor cells, hindering the application of ferroptosis stimulation in tumor treatment. In this study, a new strategy is aimed to be identified for effectively triggering immunogenic ferroptosis in pancreatic ductal adenocarcinoma (PDAC) and simultaneously stimulating antitumor immune responses...
April 9, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
#9
JOURNAL ARTICLE
Shao-Wei Hua, Jie Wang, Zi-Jin Zhao, Feng-Yu Tian, Meng Zhao, Yu-Xin Wang, Rui-Qing Zhang, Zhi-Qiang Han, Shi-Jue Gao, Xiao-Na Lv, Hong-Yi Li, Xin-Xin Shen, Xue-Jun Ma, Zhi-Shan Feng
OBJECTIVE: This study aimed to establish a highly sensitive and rapid single-tube, two-stage, multiplex recombinase-aided qPCR (mRAP) assay to specifically detect the khe, blaKPC-2 , and blaNDM-1 genes in Klebsiella pneumoniae. METHODS: mRAP was carried out in a qPCR instrument within 1 h. The analytical sensitivities of mRAP for khe, blaKPC-2 , and blaNDM-1 genes were tested using recombinant plasmids and dilutions of reference strains. A total of 137 clinical isolates and 86 sputum samples were used to validate the clinical performance of mRAP...
April 8, 2024: Journal of Clinical Laboratory Analysis
#10
JOURNAL ARTICLE
Urszula N Wasko, Jingjing Jiang, Tanner C Dalton, Alvaro Curiel-Garcia, A Cole Edwards, Yingyun Wang, Bianca Lee, Margo Orlen, Sha Tian, Clint A Stalnecker, Kristina Drizyte-Miller, Marie Menard, Julien Dilly, Stephen A Sastra, Carmine F Palermo, Marie C Hasselluhn, Amanda R Decker-Farrell, Stephanie Chang, Lingyan Jiang, Xing Wei, Yu C Yang, Ciara Helland, Haley Courtney, Yevgeniy Gindin, Karl Muonio, Ruiping Zhao, Samantha B Kemp, Cynthia Clendenin, Rina Sor, William P Vostrejs, Priya S Hibshman, Amber M Amparo, Connor Hennessey, Matthew G Rees, Melissa M Ronan, Jennifer A Roth, Jens Brodbeck, Lorenzo Tomassoni, Basil Bakir, Nicholas D Socci, Laura E Herring, Natalie K Barker, Junning Wang, James M Cleary, Brian M Wolpin, John A Chabot, Michael D Kluger, Gulam A Manji, Kenneth Y Tsai, Miroslav Sekulic, Stephen M Lagana, Andrea Califano, Elsa Quintana, Zhengping Wang, Jacqueline A M Smith, Matthew Holderfield, David Wildes, Scott W Lowe, Michael A Badgley, Andrew J Aguirre, Robert H Vonderheide, Ben Z Stanger, Timour Baslan, Channing J Der, Mallika Singh, Kenneth P Olive
Broad-spectrum RAS inhibition holds the potential to benefit roughly a quarter of human cancer patients whose tumors are driven by RAS mutations1,2 . RMC-7977 is a highly selective inhibitor of the active GTP-bound forms of KRAS, HRAS, and NRAS, with affinity for both mutant and wild type (WT) variants (RAS(ON) multi-selective)3 . As >90% of human pancreatic ductal adenocarcinoma (PDAC) cases are driven by activating mutations in KRAS4 , we assessed the therapeutic potential of the RAS(ON) multi-selective inhibitor RMC-7977 in a comprehensive range of PDAC models...
April 8, 2024: Nature
#11
JOURNAL ARTICLE
Aurora Piazza, Vittoria Mattioni Marchetti, Alessandra Bielli, Gherard Batisti Biffignandi, Francesca Piscopiello, Riccardo Giudici, Livia Tartaglione, Marco Merli, Chiara Vismara, Roberta Migliavacca
INTRODUCTION: Aim of the study was the molecular characterization of 21 ceftazidime/avibactam resistant (CZA-R) Klebsiella pneumoniae strains, collected in the period October 2021-March 2022 from an Intensive Care COVID Unit in a Northern Italian Hospital. METHODS: After growth on selective/chromogenic culture media and susceptibility tests assessment, resistance genes content was ascertained for all the isolates by the HybriSpot 12 multiplexing, PCR and Whole-Genome Sequencing (WGS)...
March 29, 2024: Journal of Microbiology Immunology and Infection
#12
JOURNAL ARTICLE
Francisco González-Espinosa, Vincenzo Di Pilato, Francisco Magariños, Jose Di Conza, Gian Maria Rossolini, Gabriel Gutkind, Marcela Radice, Daniela Cejas
OBJECTIVE: to describe at genomic level nine carbapenemase-producing Klebsiella pneumoniae ST307 (Kp-ST307) clinical isolates recovered in Buenos Aires during 2017-2021, investigating their resistome, virulome and phylogeny. METHODS: Antimicrobial susceptibility was determined according to CLSI. Genomic DNA was sequenced by Illumina MiSeq and analyzed using SPAdes, PROKKA and Kleborate. Phylogeny of 355 randomly selected Kp-ST307 genomes and those from nine local isolates was inferred by a maximum-likelihood approach...
April 5, 2024: Journal of Global Antimicrobial Resistance
#13
JOURNAL ARTICLE
Xingwei Li, Haiping Zhong, Shujing Zheng, Jingqing Mu, Na Yu, Shutao Guo
Efficient intratumoral penetration is essential for nanomedicine to eradicate pancreatic tumors. Although nanomedicine can enter the perivascular space of pancreatic tumors, their access to distal tumor cells, aloof from the vessels, remains a formidable challenge. Here, we synthesized an acid-activatable macromolecular prodrug of floxuridine (FUDR)-poly(FUDR-ketal), engineered a micellar nanomedicine of FUDR, and intravenously co-administered the nanomedicine with the tumor-penetrating peptide iRGD for enhanced treatment of pancreatic tumor...
April 2, 2024: Journal of Controlled Release
#14
JOURNAL ARTICLE
Jinlan Zhou, Gangfeng Yan, Chengkang Tang, Jing Liu, Pan Fu, Li Ding, Weiwei Yang, Yan Guo, Chuanqing Wang, Guoping Lu, Fupin Hu
Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses immense threats to the health of infected patients worldwide, especially in children. This study reports the infection caused by CRKP in a pediatric intensive care unit (PICU) child and its drug-resistant mutation during the treatment. Twelve Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae strains were isolated from the child. Broth microdilution method, plasmid transformation assay, and whole genome sequencing (WGS) were performed to investigate the antimicrobial susceptibility, resistance mechanisms, and genetic structural features of CRKPs...
April 1, 2024: International Journal of Antimicrobial Agents
#15
JOURNAL ARTICLE
Yasmine H Tartor, Ahmed M Ammar, Adel Abdelkhalek, Khlood A Hassan, Asmaa Shaker, Shimaa S Elnahriry, Omid Nekouei, Ibrahim Elsohaby
One of the most important emerging health problems is the increasing role of animals in the rapid global rise in resistance to last-resort antibiotics, such as carbapenems. However, there is limited information on the role of pet animals in harboring and spreading pandrug-resistant (PDR) carbapenemase-producing Enterobacterales (CPE), especially in Egypt. This cross-sectional study was conducted to screen for CPE in healthy and diseased pets using phenotypic and molecular methods and the NG-Test CARBA 5 immunochromatographic assay...
2024: Frontiers in Cellular and Infection Microbiology
#16
JOURNAL ARTICLE
Nuno Alexandre Faria, Tiago Touret, Alexandra S Simões, Carlos Palos, Soraia Bispo, José Melo Cristino, Mário Ramirez, João Carriço, Margarida Pinto, Cristina Toscano, Elsa Gonçalves, Maria Luísa Gonçalves, Augusto Costa, Mariana Araújo, Aida Duarte, Hermínia de Lencastre, Mónica Serrano, Raquel Sá-Leão, Maria Miragaia
Carbapenem-resistant Klebsiella pneumoniae complex (CR-KP) are a public health concern, causing infections with a high mortality rate, limited therapeutic options and challenging infection control strategies. In Portugal CR-KP rate has increased steeply, but the factors associated to this expansion are poorly explored. To address this question we compared, by phylogenetic and resistome analysis, the draft genomes of 200 CR-KP isolates collected in 2017-2019 from five hospitals in the Lisbon region, Portugal...
March 28, 2024: Journal of Hospital Infection
#17
JOURNAL ARTICLE
Qian Wang, Yue Liu, Ran Chen, Meng Zhang, Zaifeng Si, Yueling Wang, Yan Jin, Yuanyuan Bai, Zhen Song, Xinglun Lu, Mingju Hao, Yingying Hao
BACKGROUND: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) co-producing blaKPC and blaNDM poses a serious threat to public health. This study aimed to investigate the mechanisms underlying the resistance and virulence of CR-hvKP isolates collected from a Chinese hospital, with a focus on blaKPC and blaNDM dual-positive hvKP strains. METHODS: Five CR-hvKP strains were isolated from a teaching hospital in China. Antimicrobial susceptibility and plasmid stability testing, plasmid conjugation, pulsed-field gel electrophoresis, and whole-genome sequencing (WGS) were performed to examine the mechanisms of resistance and virulence...
March 29, 2024: Annals of Clinical Microbiology and Antimicrobials
#18
JOURNAL ARTICLE
Virender Kumar, Bharti Sethi, Dalton W Staller, Prakash Shrestha, Ram I Mahato
Approximately 90% of pancreatic cancer (PC) contain KRAS mutations. Mutated KRAS activates the downstream oncogenic PI3K/AKT and MEK signaling pathways and induces drug resistance. However, targeting both pathways with different drugs can also lead to excessive toxicity. ONC201 is a dual PI3K/AKT and MEK pathway inhibitor with an excellent safety profile that targets death receptor 5 (DR5) to induce apoptosis. Gemcitabine (GEM) is a first-line chemotherapy in PC, but it is metabolically unstable and can be stabilized by a prodrug approach...
March 29, 2024: Cell Death Discovery
#19
JOURNAL ARTICLE
Ying Jing, Sufei Yu, Zhaolun Li, Jie Ma, Luwei Wang, Lianhua Yu, Zhiwei Song, Huimin Chen, Zhenghai Wu, Xinhua Luo
OBJECTIVES: Herein, we detected one multidrug-resistant Aeromonas hydrophila strain K522 co-carrying two blaKPC genes together with a novel chromosomal integrative and mobilizable element (IME) Tn7548 from China. To reveal the genetic characteristics of the novel reservoir of blaKPC-2 and IME in Aeromonas, a detailed genomic characterization of K522 was performed, and a phylogenetic analysis of Tn7412-related IMEs was carried out. METHODS: Carbapenemases were detected by using the immunocolloidal gold technique and antimicrobial susceptibility was tested by using VITEK 2...
March 27, 2024: Journal of Global Antimicrobial Resistance
#20
JOURNAL ARTICLE
Fushan Zhang, Leyuan Li, Yuxin Zhao, Huiyue Dong, Buhui Zhao, Xiaoyu Zhao, Ziwei Xia, Leizi Chi, Yan Wang, Ruichao Li, Shangshang Qin, Xiangjing Fu
INTRODUCTION: Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) strains combining virulence and multidrug resistance (MDR) features pose a great public health concern. The aim of this study is to explore the evolutionary characteristics of virulence in CR-HvKP by investigating the genetic features of resistance and virulence hybrid plasmids. METHODS: The resistance and virulence phenotypes were determined by using antimicrobial susceptibility testing and the mouse bacteremia infection model, respectively...
2024: Frontiers in Microbiology
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